(A) Transmission electron microscopy images and (B) histogram and normal distribution from these images for (a) ZnOSM20(−), (b) ZnOSM20(+), (c) ZnOAE100(−),
The authors demonstrate a high performance electrically controlled birefringence (ECB) mode with solution-derived La{sub 2}O{sub 3} films at various molar concentrations. Uniform and homogeneous liquid crystal (LC) alignment was spontaneously achieved on the La{sub 2}O{sub 3} films for lanthanum concentrations at ratios greater than and equal to 0.2. A preferred orientation of LC molecules appeared along the filling direction, and the LC alignment was maintained via van der Waals force by nanocrystals of the La{sub 2}O{sub 3} films. The LC alignment mechanism was confirmed by x-ray photoelectron spectroscopy and high-resolution transmission electron microscopy analysis. Superior electro-optical characteristics of the ECB cells constructed with solution-derived La{sub 2}O{sub 3} films were observed, which suggests that the proposed solution-derived La{sub 2}O{sub 3} films have strong potential for use in the production of advanced LC displays. ...
An in situ transmission electron microscopy (TEM) analysis of a solid electrolyte, Cu-GeS, during resistance switching is reported. Real-time observations of the filament formation and disappearance process were performed in the TEM instrument and the conductive-filament-formation model was confirmed experimentally. Narrow conductive filaments were formed corresponding to resistance switching from high- to low-resistance states. When the resistance changed to high- resistance state, the filament disappeared. It was also confirmed by use of selected area diffractometry and energy-dispersive x-ray spectroscopy that the conductive filament was made of nanocrystals composed mainly of Cu ...
Solid-state nucleation of Si nanocrystals in a SiO{sub 2} bilayered matrix was observed at temperatures as low as 450 °C. This was achieved by aberration corrected high-resolution transmission electron microscopy (HRTEM) with real-time in-situ heating up to 600 °C. This technique is a valuable characterization tool especially with the recent interest in Si nanostructures for light emitting devices, non-volatile memories, and third-generation photovoltaics which all typically require a heating step in their fabrication. The control of size, shape, and distribution of the Si nanocrystals are critical for these applications. This experimental study involves in-situ observation of the nucleation of Si nanocrystals in a SiO{sub 2} bilayered matrix fabricated through radio frequency co-sputtering. The results show that the shapes of Si nanocrystals in amorphous SiO{sub 2} bilayered matrices are irregular and not spherical, in contrast to many claims in the literature. Furthermore, the Si ...
Luminescence-tunable multicolored LaF3:xCe3+,xGd3+,yEu3+ (x = 5; y = 1, 5, 10, and 15 mol%) nanoparticles have been synthesized via a low cost polyol method. Powder X-ray diffraction and high-resolution transmission electron microscopy studies confirm the hexagonal phase of the LaF3:xCe3+,xGd3+,yEu3+ nanopho
Single crystal strontium titanate (SrTiO3) is used in the microelectronics industry as insulating layers in dynamic random access memories, ferroelectric thin film structures, and high-Tc superconductor devices. Strontium titanate and other titanate ceramics have also been proposed for immobilization of nuclear waste. In many of these applications, knowledge of dynamic recovery and nanostructure evolution is critical. The current work studied the response of ion-beam induced amorphous layers in SrTiO3 to electron beam (e-beam) irradiation. Amorphous surface layers were formed on single crystals of SrTiO3 by 1.0 MeV irradiation with gold ions at 400 K. The microstructural features, electron diffraction patterns, high-resolution transmission electron microscopy (TEM) images and recrystallization rates of the samples were determined at 300, 353 and 393 K using TEM. Following exposure of the amorphous layers to TEM e-beams, the amorphous thickness decreases with exposure time, indicating in-situ ...
Fuel cells, secondary batteries and capacitors are among many promising energy storage devices. In particular, supercapacitors have attracted much attention because of their long life cycle and high power density. Graphene/nickel ferrite(G-NF) based supercapacitors were successfully fabricated through a one-step facile solvothermal route. Effects of synthesis conditions i.e. solvothermal time and temperature, on the powder particle characteristics were evaluated using x-ray photoelectron spectroscopy (XPS), powder x-ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM). Fast Fourier transformation (FFT) patterns were also recorded on the HRTEM microscope to determine the lattice and crystallinity of the nanocomposites. Structural and chemical studies proved that increasing the solvothermal duration and temperature leads to improved crystallinity of NiFe2O4phase as well as higher degree of reduction of graphene oxide to graphene. The electrochemical measurements showed that
In this work, a new nanocomposite in which polyaniline (PANI) is encapsulated in ordered mesoporous carbon (CMK-3 type) has been synthesized. The aniline monomer was introduced into hosts from the vapor phase to avoid the formation of PANI layers on the outer surface. The structure and morphology of the nanocomposite were characterized by X-ray diffraction (XRD), N2 adsorption=desorption, FT-IR and Raman spectra, high-resolution transmission electron microscopy (HRTEM), and thermogravimetric analysis (TGA). The results show that PANI is almost formed in the channels of CMK-3 and the nanocomposite retains the ordered mesostructure. Encapsulated PANI exhibits better thermal stability than pure PANI because of confinement effect in the channel system. The conductivity of PANI=CMK-3 nanocomposite containing 8.4 wt% PANI is 0.78 S=cm at room temperature ...
Abstract The study of properties of low-dimensional materials is currently one domain of aberration-corrected high-resolution transmission electron microscopy at lower accelerating voltages of 80keV. The susceptibility to radiation damage both challenges the interpretation of the structure at the atomic level and allows at the same time the study of the dynamics of transformations. We report atom-by-atom on the direct formation of structures such as fullerenes, nanoribbons, nanotubes and 2D-layers, which are stimulated by the electron beam and/ or by heat. Moreover, under the influence of the e-beam during its imaging process, the creation and motion of point defects, dislocations, grain boundaries and phase transitions can be observed and allow an unprecedented view into their atomic nature and properties, if combined by atomistic simulations. Imaging the pristine structure of beam-sensitive materials, however, still remains a challenging task. We show that fullerenes inside carbon nanotubes at 20kV
Barium ferrate is the chemical compound of formula BaFeO4. This is a rare compound containing iron in the +6 oxidation state. The ferrate (VI) ion has two unpaired electrons, making it paramagnetic. It is isostructural with BaSO4, and contains the tetrahedral [FeO4]2− anion. The ferrate(VI) anion is paramagnetic due to its two unpaired electrons and it has a tetrahedral molecular geometry. X-ray diffraction has been used to determine the orthorhombic unit cell structure (lattice vectors a ≠ b ≠ c, interaxial angles α=β=γ=90°) of nanocrystalline BaFeO4. It has a pnma space group (point group: D2h) with lattice parameters a=0.8880 nm, b=0.5512 nm and c=0.7214 nm. The accuracy of the X-Ray diffraction data has been verified by the lattice fringe intervals from High-Resolution Transmission Electron Microscopy (HRTEM) and cell parameters calculated from Selected Area Diffraction (SAED). Infra-red peaks of barium ferrate are observed at 870, 812, 780 cm−1. BaFeO4 has a magnetic moment of ...
Scanning force microscopy (AFM) and high-resolution transmission electron microscopy (HRTEM) have been used to investigate the complex topography evolution of Si surfaces during low-energy ion beam erosion. Depending on ion-beam parameters, a variety of different topographies can develop on the surface. At oblique ion-incidence angles, nanodots are formed for ion energies >=300 eV upon sample rotation. Properly chosen parameters of the broad-beam ion source result in dots possessing a very high degree of lateral ordering with a mean dot size λ∼30 nm. Both, degree of ordering and size homogeneity of these nanostructures increases with erosion time leading to the most ordered self-organized patterns on Si surfaces reported thus far ...
A series of semi-crystalline, wide band gap (WBG) photovoltaic polymers were synthesized with varying number and topology of fluorine substituents. To decrease intramolecular charge transfer and to modulate the resulting band gap of D-A type copolymers, electron-releasing alkoxy substituents were attached to electron-deficient benzothiadiazole (A) and electron-withdrawing fluorine atoms (0-4F) were substituted onto a 1,4-bis(thiophen-2-yl)benzene unit (D). Intra- and/or interchain noncovalent Coulombic interactions were also incorporated into the polymer backbone to promote planarity and crystalline intermolecular packing. The resulting optical band gap and the valence level were tuned to 1.93-2.15 eV and −5.37 to −5.67 eV, respectively, and strong interchain organization was observed by differential scanning calorimetry, high-resolution transmission electron microscopy and grazing incidence X-ray scattering measurements. The number of fluorine atoms and their position significantly ...
The crystallographic and electronic structures of (010) APB of L12 Co3Al0.75TM0.25 are studied by high-resolution transmission electron microscopy and first-principles calculations. Effects of solute atoms (TM = Cr, Hf, Mo, Ni, Re, Ru, Ta, Ti, W and Y) on the formation energy, lattice parameters/distortion, magnetism, and bonding strength of the (010) APB in Co3Al0.75TM0.25 are obtained from first-principles calculations. Comparing to the equilibrium volume of Co3Al, it is found that the volume change of the Co3Al0.75TM0.25 with and without the presence of APB increases linearly with the volume of the corresponding FCC elements, indicating the contribution of the solute atoms on lattice distortion of bulk and (010) APB. Particularly, the strong dependence of the APB energy on the composition is comprehensively discussed together with the available experimental and theoretical data in the literature. The negative (010) APB energy indicates that the formation of (010) APB could stabilize the ...
阿南 義弘 , 浅香 透 , ZHIGADLO N.D. , 室町 英治 , 木本 浩司 , 松井 良夫 電子顕微鏡 35, 328, 2000-05-01 参考文献2件 ...
A novel image processing method based on mathematical morphology is applied in order to characterize the nanostructure of carbide-derived carbons (CDCs) observed using high resolution transmission electron microscopy (HRTEM). The analysis provides information about the shape and disordered arrangement of the defective polyaromatic units forming the CDC nanostructure. Individual fringes, basic structural units, and continuous domains are analysed. Hierarchical polycarbosilane-based CDCs obtained at different pyrolysis/chlorination temperatures are investigated. The information collected is interpreted with respect to the different synthesis conditions. This analysis is supported by Raman spectroscopy measurements and porosity evaluation with nitrogen (-196 degrees C) and carbon dioxide (0 degrees C) physisorption. The CDCs show only minor differences in the carbon nanostructures. The HRTEM image analysis is sensitive enough to illuminate the slight variations. An increase in carbon ordering at higher
We report the nanostructure study results, mainly based on plan-view transmission electron microscopy (TEM) on the coalescence process during the overgrowth by metalorganic chemical vapor deposition of GaN nanocolumns grown by molecular beam epitaxy. In cross-section scanning electron microscopy images, one can observe a two-stage coalescence overgrowth process. First, a group of nearby nanocolumns is merged into a thicker column. One of the possible merging processes is the growth of a bridging domain between two columns for their connection. The thicker columns are then developed into horn-shaped structures for the second-stage coalescence. Because different columns may have different crystal orientations, stacking faults can be formed for implementing the coalescence between two domains. Such stacking faults around the boundaries of merged domains represent one of the major kinds of defect after the threading dislocation density is reduced based on the nanocolumn growth technique.. ©2013 ...
Infrared absorption spectra of polyhedral and platelet oxygen precipitates in silicon are analyzed using a modified Day-Thorpe approach [J. Phys.: Condens. Matter 11, 2551 (1999)]. The aspect ratio of the precipitates is determined by transmission electron microscopy analysis. The reduced spectral function and the stoichiometry of the precipitate are extracted from the absorption spectra and the amount of precipitated interstitial oxygen. The experimental absorption spectra can be divided in a set with a Frohlich frequency of around 1100 cm(-1) and in a set with a Frohlich frequency between 1110 and 1120 cm(-1). It is shown that the shift in the Frohlich frequency is not due to a differing stoichiometry, but to the detailed structure of the reduced spectral function. Inverse modeling of the spectra suggests that the oxide precipitates consist of substoichiometric SiOgamma with gamma=1.17+/-0.14. (C) 2002 American Institute of Physics. ...
David Nam, Judith Mantell, David Bull, Paul Verkade, Alin Achim. The following work presents a graphical user interface (GUI), for automatic segmentation of granule cores and membranes, in transmission electron microscopy images of beta cells. The system is freely available for academic research. Two test images are also included. The highlights of our approach are:. ...
This thesis is focused on two intermetallic systems Bi1-xSbx and CaAl2-xZnx. Bi and Sb transform into a peculiar incommensurate composite structure under pressure and our intention was to examine the structure and how alloying influences this incommensurate structure. Our investigation was obscured by the occurrence of a phase separation, which accompanied the transition A7 → Bi III. Most remarkable is the finding that structural parameters of phases with the Bi III composite structure were observed to be almost unaffected by pressure and composition effects.. Secondly, phase and structural stability relations within the AB2 pseudo-binary system CaAl2-xZnx was studied. In CaAl2-xZnx, the C36-type Laves phase was observed for the first time, but not the C14-type, and a VEC induced structural transition C15-type → C36-type takes place with increasing x. The exchange of Al by Zn decreases the size of the B-type atom network surrounding Ca and at concentrations x , 0.95, the Laves phase ...
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Lung Ultrastructure. Effect of H2S on mitochondrial ultrastructure in lung cells, as determined by transmission electron microscopy analysis, in LPS-Induced ALI
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Mitochondrial morphology is definitely remodeled by fusion and fission in cells dynamically, and dysregulation of the procedure is implicated in tumorigenesis closely. success. Furthermore, treatment with mitochondrial department inhibitor-1 considerably suppressed tumor development within an in vivo xenograft nude mice model. Our results demonstrate that increased mitochondrial fission plays a critical role in regulation of HCC cell survival, which provides a strong evidence for Rabbit Polyclonal to Cytochrome P450 51A1. this process as drug target in HCC treatment. = 0.024, 0.017 and 0.007, respectively, Fig.?1E to G). Figure 1. Mitochondrial dynamics in HCC tissues and their effects on prognosis of HCC patients. (A) Representative transmission electron microscopy images of mitochondrial network in paired tissues from HCC patients (n=15). Asterisks, AZD1480 arrows and triangles indicate … Mitochondrial fission promotes the mitochondrial function and survival of HCC cells both in vitro and in ...
We have published another manuscript, Robust Segmentation based Tracking using an Adaptive Wrapper for Inducing Priors. This manuscript describes the work on adaptive tracing and proposes an algorithm that adapts a generic tracing algorithm to an application of interest. In our specific case, it is boundaries of cells in high frequency space in transmission electron microscopy images. But the approach in this paper is applicable to biological, medical, remote sensing and surveillance data as well utilizing priors specific to the application. The co-authors on the paper are: Vignesh Jagadeesh, James Anderson, myself, Robert E. Marc, Steven K Fisher and B.S Manjunath.. ...
High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section through a Chinese hamster ovary cell at metaphase showing a chr...
Available in TEM mode for approved users. SEM/STEM mode is "in progress".. Transmission Electron Microscope (60-100KeV) with 2Mpix side-mounted AMT digital camera. STEM / SEM capability.. Fees: Internal $28/hr, $67/assisted. External $41/hr, $88/hr assisted. ...
Latest report titled Global Transmission Electron Microscope Market 2015-2019 - Market Research & Analysis is now available for $2500 at ResearchMoz
(a) Electron backscatter diffraction (EBSD) phase image showing the lamella microstructure of layered austenite grains embedded in tempered martensite matrix. (b) The dislocation structures in martensite as enlarged in transmission electron microscopy (TEM) image. (c) TEM image showing the elongation of dislocation cell structure after the 8% tensile strain. (d) TEM image confirming the transformation of metastable austenite to martensite after 16% tensile strain.
a TEM image of CD. b HRTEM of CD. c IFFT image of CD. d TEM image of PNC4.0. e HRTEM of PNC4.0. f IFFT of PNC4.0. g Size distribution of CDs ...
We have developed a self-contained liquid cell, which allows imaging through liquids using a transmission electron microscope. Dynamic...
Ferro-anthophyllite. Comments: TEM image showing a small percentage of Pnma ferroanthophyllite of similar composition is present as thin slabs intergrown parallel to (100) of grunerite and as individual -size crystals ...
First-rate scientific equipment, facilities, and other resources support biology teaching and research at Gettysburg, enabling students and faculty to study and practice biology using contemporary tools of biology. The Biology Department has excellent imaging resources, including both a scanning electron microscope (SEM) and a transmission electron microscope (TEM), the latter of which is seldom found at colleges of Gettysburgs size. The greater resolution of the TEM allows studies of cellular sections and of smaller entities such as viruses.. Within and near the Biology Departments home in the McCreary Hall/Science Center complex youll find the following:. ...
The Microscopy Core Facility, on the ground floor of McLeans Mailman Research Center, houses some of the most sophisticated magnifying instruments available. Their capabilities astound: the transmission electron microscope (TEM), for example, can magnify a spot on a rodent brain slice up to 300,000 times with impeccable resolution, revealing even the infinitesimal junctions-the synapses-that electrical impulses cross in traveling from one neuron to another.
Yüksek Çözünürlüklü Dairesel Aydınlık Alan ve Karanlık Alan Görüntüleme Tekniklerinin ve Atom Kolonları Ölçeğinde Kimyasal Analiz Metotlarının Titanat Esaslı Seramiklere Uygulaması: Soğuk Gerilim Koparımlı Elektron Tabancaya Sahip Cs-hataları Düzeltilmiş Taramalı ve Geçirgenli Elektron Mikroskobunun (STEM) Avantajları. Application of High Resolution Annular Dark and Bright Field Imaging and Atomic Column Resolved Chemical Analysis on Titanate Based Ceramics: The Advantage of a Cs-corrected Scanning Transmission Electron Microscope (STEM) with a Cold-FEG. , Co-director: Gülgün, Mehmet Ali , Starting Date: December 2012, Duration:5 month(s ...
White-colored materials synthesized by a thermal annealing of milled GaN powders at 930 °C in a nitrogen atmosphere were identified to be monoclinic β-Ga[sub 2]O[sub 3] nanowires by x-ray diffraction and scanning electron microscopy. High-resolution transmission electron microscopy revealed that these nanowires are single nanocrystals, and energy dispersive x-ray indicated that these nanomaterials are free of any metals. In addition, bundles of these crystalline nanowires in the rectangular-pole shape are a few centimeters in length.© 2002 American Institute of Physics ...
In this work, Ag-doping TiO2 nanotubes were prepared and employed as the photocatalyst for the degradation of toluene. The TiO2 nanotube powder was produced by the rapid-breakdown potentiostatic anodization of Ti foil in chloride-containing electrolytes, and then doped with Ag through an incipient wetness impregnation method. The samples were characterized by scanning electron microscope, high-resolution transmission electron microscopy, X-ray diffraction, surface photovoltage measurements, X-ray photoelectron spectroscopy and N2 adsorption. The nanotubular TiO2 photocatalysts showed an outer diameter of approximately 40nm, fine mesoporous structure and high specific surface area. The photocatalytic activity of Ag-doping TiO2 nanotube powder was evaluated through photooxidation of gaseous toluene. The results indicated that the degradation efficiency of toluene could get 98% after 4h reaction using the Ag-doping TiO2 nanotubes as the photocatalyst under UV light illumination, which was higher ...
TY - JOUR. T1 - Synergistic helium and deuterium blistering in tungsten-tantalum composites. AU - Dias, M.. AU - Mateus, A. R.. AU - Catarino, N.. AU - Franco, N.. AU - Nunes, D.. AU - Correia, J. B.. AU - Carvalho, P. A.. AU - Hanada, K.. AU - Sârbu, C.. AU - Alves, E.. N1 - sem pdf conforme despacho. Portuguese Science and Technology Foundation (FCT) - PTDC/CTM/100163/2008 ;PEST-OE/CTM-UI0084/2011 ; PEST-C/CTM/LA0025/2013-14 ; FCT Grant - SFRH/BPD/68663/2010.. PY - 2013. Y1 - 2013. N2 - Tungsten-tantalum composites with 10 and 20 at.% Ta were prepared by ball milling W powder with Ta fibers and by consolidating the milled materials with spark plasma sintering. The composites were implanted at room temperature with He+ (30 keV with a fluence 5 × 1021at/m2) and/or D+ (15 keV with a fluence 5 × 1021 at/m 2) ion beams. The materials were studied by scanning and high-resolution transmission electron microscopy, both coupled with energy dispersive X-ray spectroscopy, and by X-ray diffraction, ...
Stable luminescent silver clusters in nanosized EMT zeolite suspension were prepared and directly observed with high-resolution transmission electron microscopy (HRTEM). The luminescence of the Ag clusters remains stable in time due to their stabilization within the sodalite cages (0.7 nm) of the EMT zeolite nanocrystals. In addition to the experimental results, the first principle Density Functional Theory (DFT) computations showed that hydrated neutral clusters up to octamer (Ag8) with a diameter of 0.47 nm were stabilized in the sodalite cages of the EMT zeolite, trough binding of silver atom(s) to the zeolite oxygen(s). The silver clusters exhibit molecular-like emission properties (lem = 395 nm and t1/2 ≤ 1 ns) that are in a good agreement with the HRTEM and DFT results. The stabilization of charge silver species in the form of weakly interacting dimer or trimer was observed too, which was based on the microsecond lifetime of the emission band measured at 545 nm. The high stability combined with
In this work, the desorption of furfural, which is a competitive intermediate during the production of biofuel and valuable aromatic compounds, was studied using pure alumina, as well as alumina impregnated with iron and platinum oxides both individually and in combination, using thermogravimetric analysis (TGA). The bimetallic sample exhibited the lowest desorption percentage for furfural. High-resolution transmission electron microscopy (HRTEM) imaging revealed the intimate connection between the iron and platinum oxide species on the alumina support. The mechanism of furfural desorption from the Pt-Fe/Al2O3 0.5%-0.5% sample was determined using physisorbed furfural instead of chemisorbed furfural; this mechanism involved the oxidation of the C=O group on furfural by the catalyst. The oxide nanoparticles on γ-Al2O3 support helped to stabilize the furfural molecule on the surface.
Chlamydia trachomatis serovars D-K are the leading cause of bacterially-acquired sexually transmitted infections in the United States. As an obligate intracellular pathogen, C. trachomatis infects columnar epithelial cells of the genital mucosae and can cause deleterious sequelae such as pelvic inflammatory disease, infertility, and ectopic pregnancy. Several chlamydial antigens reach the host cell cytosol prior to the natural release of chlamydiae at the end of the developmental cycle. While some of these extra-inclusion antigens traffic to the host cell surface, others remain intracellular where they are proposed to influence vital host cell functions and antigen trafficking and presentation. The research herein examines the escape and trafficking of the immunodominant chlamydial antigens MOMP, LPS, and cHsp60 within C. trachomatis serovar E-infected polarized human endometrial epithelial cells. Studies using high-resolution transmission electron microscopy (TEM) and immuno-TEM report the novel escape
Invited Talks 29. Nanosessions 69. Nanosession: 2D electron systems - Atomic configurations 71. Nanosession: 2D electron systems - Correlation effects and transport 81. Nanosession: 2D electron systems - Electronic structure and field effects 89. Nanosession: Calorics 99. Nanosession: Topological effects 109. Nanosession: Mott insulators and transitions 115. Nanosession: Advanced spectroscopy and scattering 123. Nanosession: High-resolution transmission electron microscopy 133. Nanosession: New technologies for scanning probes 143. Nanosession: Phase change materials 155. Nanosession: Phase change memories 163. Nanosession: Scanning probe microscopy on oxides 177. Nanosession: Logic devices and circuit design 185. Nanosession: Neuromorphic concepts 197. Nanosession: Electrochemical metallization memories 207. Nanosession: Valence Change Memories - redox mechanism and modelling 219. Nanosession: Valence Change Memories - a look inside 233. Nanosession: Variants of resistive switching ...
Observations on the interaction of anthracycline antibiotics with the plasma membrane, performed by morphological, ultrastructural, microanalytical and spectroscopic methods, are reported and discussed in this review. Scanning and transmission electron microscopy analysis indicates that doxorubicin (DOX) induces dose-dependent modifications of the cell morphology and membrane ultrastructure of human erythrocytes. The formation of intramembrane particle-free domains on both the fracture faces of the plasma membrane suggests that the DOX molecules become incorporated within the lipid bilayer. Electron energy-loss spectroscopy measurements reveal a reduction in the P/C ratio in treated membranes, probably due to a phospholipid dilution following the incorporation of DOX molecules. The radiowave dielectric spectroscopy indicates modifications induced by DOX in the passive electrical properties of the membrane. In particular, the decrease in membrane conductivity suggests that the interaction of ...
This thesis describes the design of enzymatic systems forthe modification of cellulose fiber surfaces. A single-enzymeapproach employed a conjugate between theNeocallimastix patriciarumCel6A cellulose-bindingmodule and lipase B fromCandida antarctica(CBM-CALB) to enhance theaccessibility of the enzyme to the target hydroxyl groups. Thecharacteristics of the N.patriciarumfamily 1 CBM along with other wild type andmutated CBMs were investigated. The presence of one tryptophanamong the three aromatic residues on the binding face of afamily 1 CBM enhanced its binding to cellulose while a secondtryptophan did not lead to any further improvement.Transmission electron microscopy analysis indicated thatvarious family 1 and 3 CBMs bound to the exposed hydrophobicsurfaces of cellulose crystals.. Proteolytically stable expression of CBM-CALB in Pichiapastoris was achieved in fed-batch bioreactor cultivationsusing two strategies. Firstly, the linker joining the fusionpartners was engineered to enhance the ...
TY - JOUR. T1 - Direct Comparison of Standard Transmission Electron Microscopy and Cryogenic-TEM in Imaging Nanocrystals Inside Liposomes. AU - Li, Tang. AU - Nowell, Cameron J.. AU - Cipolla, David. AU - Rades, Thomas. AU - Boyd, Ben J.. PY - 2019/4/1. Y1 - 2019/4/1. N2 - The use of electron microscopy techniques in the understanding of shape and size of nanoparticles are commonly applied to drug nanotechnology, but the type of microscopy and suitability for the particles of interest can have a significant impact on the result. The size and shape of the nanoparticles are crucial in clinical applications; however, direct comparison of the results from standard transmission electron microscopy (TEM) and cryo-TEM have rarely been reported. As a useful case for comparison, liposomal drug nanocrystals are studied here. In this study, the effect of thawing temperature on the size and shape of the ciprofloxacin nanocrystals was determined. A quantitative standard TEM assay was developed to allow for ...
Other animals such as birds and insects achieve ultrablack coloration by embedding melanin in a matrix of keratin or chitin that helps the pigment trap and absorb light. But transmission electron microscopy images of fish skin reveal only densely packed layers of melanosomes-cellular structures packed full of melanin, suggesting that the pigment alone is responsible for the effect. The fish use melanosomes of "just the right size and shape so that any light they dont absorb immediately bounces sideways and gets absorbed by its neighbor," Osborn says.. ...
We present a novel approach to measuring distances between objects in images, suitable for information-rich object representations which simultaneously capture several properties in each image pixel. Multiple spatial fuzzy sets on the image domain, unified in a vector-valued fuzzy set, are used to model such representations. Distance between such sets is based on a novel point-to-set distance suitable for vector-valued fuzzy representations. The proposed set distance may be applied in, e.g., template matching and object classification, with an advantage that a number of object features are simultaneously considered. The distance measure is of linear time complexity w.r.t. the number of pixels in the image. We evaluate the performance of the proposed measure in template matching in presence of noise, as well as in object detection and classification in low resolution Transmission Electron Microscopy images.. ...
The self-assembly and antimicrobial activity of two novel arginine-capped bola-amphiphile peptides, namely RA6R and RA9R (R, arginine; A, alanine) are investigated. RA6R does not self-assemble in water due to its high solubility, but RA9R self-assembles above a critical aggregation concentration into ordered nanofibers due to the high hydrophobicity of the A9block. The structure of the RA9R nanofibers is studied by cryogenic transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS). Circular dichroism spectroscopy shows that both RA6R and RA9R adopt coil conformations in water at low concentration, but only RA9R adopts a β-sheet conformation at high concentration. SAXS and differential scanning calorimetry are used to study RA6R and RA9R interactions with a mixed lipid membrane that models a bacterial cell wall, consisting of multilamellar 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol/1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine vesicles. Cytotoxicity studies show ...
Progress in Powder Metallurgy: In-Situ TEM Observation on Phase Formation of TiO|sub|2|/sub| Nanoparticle Synthesized by Flame Method
Learn how your ultrastructural investigations can benefit from modern electron microscope techniques. Correlative microscopy provides you deeper insights about cell structure by combination of light and scanning electron microscopy methods.