View Stock Photo of Smallpox Virus Single Virion As Seen By Negative Stain Electron Microscopy. Find premium, high-resolution photos at Getty Images.
We have developed techniques for studying patch-clamped membranes inside glass pipettes using high voltage electron microscopy (HVEM). To preserve the patch structure with the least possible distortion, we rapidly froze and freeze dried the pipette tip. The pipette is transparent for more than 50 microns from the tip. HVEM images of patches confirm light microscopy observations that the patch is not a bare bilayer, but a membrane-covered bleb of cytoplasm that may include organelles and cytoskeleton. The membrane that spans the pipette is commonly tens of micrometers from the tip of the pipette and occasionally as far as 100 microns. The structure of patches taken from a single cell type is variable but there are consistent differences between patches made from different cell types. With suction applied to the pipette before seal formation, we have seen in the light microscope vesicles swept from the plasmalemma up the pipette. These vesicles are visible in electron micrographs, particularly ...
1963 Conference on Electron Microscopy, Gliwice, chairman: S. Gorczyca, in Polish, 10 papers (unpublished) 1971 II Polish Conference on Electron
Electron microscopic localization of acetylcholinesterase in the superior cervical ganglion of the rat. In: Kolligátum. pp. 274-285. (1967 ...
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TY - JOUR. T1 - A structural study of tin-antimony oxide catalysts by high-resolution electron microscopy. AU - Berry, Frank J.. AU - Smith, David J.. PY - 1984/7. Y1 - 1984/7. N2 - Tin-antimony oxide catalysts prepared by the calcination of precipitates have been investigated by high-resolution electron microscopy. The exposure of the catalysts prepared at low temperatures to gaseous atmospheres containing hydrocabon and oxygen results in a segregation of antimony from the poorly crystalline rutile-type solids and the development of an amorphous material. The catalysts containing low concentrations of antimony are also partially converted to a non-rutile-type crystalline phase. Prolonged calcination in air of the used catalysts at high temperatures leads to the attainment of bulk equilibrium and the formation of solid solutions of antimony in tin(IV) oxide. Treatment of the equilibrated crystalline catalysts prepared at high temperatures in the hydrocarbon and oxygen gas stream gives rise to ...
A method for forming a zoned distribution of particulate material within a fibrous web includes a conveying step for providing a gas entrained supply of the particulate material and a segregating step centrifugally directing at least a portion of the particulate material into an accumulation region. A transferring step selectively directs particulate material from the accumulation region into a delivery gas stream to provide an intermittent flow volume of a selected quantity of particulate material from the accumulation region through a delivery conduit and into a web forming chamber. A fiberizing step provides a flow of a selected fibrous material into the web forming chamber, and a directing step controls the intermittent flow of particulate material from the delivery conduit into the forming chamber. A foraminous forming layer is disposed within the forming chamber for receiving the fibrous material and the particulate material to produce a fibrous web which includes zoned regions having selected,
Mitosis in yeast Saccharomyces cerevisiae was investigated in thick (0-25-I mum) serial sections with a high voltage electron microscope and in preparations of spheroplasts spread on a water surface. Spindle microtubules originate from a plaque-like structure called the spindle pole bosis the SPB duplicates and a set of long and short microtubules develops on each SPB. The spindle arises as the SPBs separate on the nuclear membrane adense and are not individually visible. Genetic studies, however, have indicated that there are 17 linkage groups. The number of microtubules was determined in diploid and haploid spindles on serial stereo micrographs. In diploid mitosis about 40 microtubules issue from a SPB. Most are non-continuous and often they are visibly associated with a chromatin fibre. The spindle in haploid cells is similar except that the number of microtubules is about half that in diploid cells and the SPB is smaller. The pole-to-pole microtubules vary in number from spindle to spindle, ...
Biology is a challenging and complicated mess. Understanding this challenging complexity is the realm of the biological sciences: Trying to make sense of the massive, messy data in terms of discovering patterns and revealing its underlying general rules. Among the most powerful mathematical tools for organizing and helping to structure complex, heterogeneous and noisy data are the tools provided by multivariate statistical analysis (MSA) approaches. These eigenvector/eigenvalue data-compression approaches were first introduced to electron microscopy (EM) in 1980 to help sort out different views of macromolecules in a micrograph. After 35 years of continuous use and developments, new MSA applications are still being proposed regularly. The speed of computing has increased dramatically in the decades since their first use in electron microscopy. However, we have also seen a possibly even more rapid increase in the size and complexity of the EM data sets to be studied. MSA computations had thus become a
A hemagglutination-inhibitory mucoprotein from human urine has been studied with the electron microscope. It consists of filaments, with diameters of 40 to , 240 A, composed of smaller fibrils. In the two-dimensional projection of the electron micrographs, the single fibrils often show a zig-zag course with a periodicity of 100 to 140 A; the single branch of a zig-zag measures about 60 A in length and either 20 or 40 A in width. Still thinner fibrillar elements are observable with diameters of 10 A or less. In three-dimensional aspect, the zig-zag structure might be a helix. The fibril-bundle (or filament) reveals a complicated configuration. Heat treatment at 70°C shows some indication of denaturation (e.g. filaments are shorter), whereas at 80°C almost complete degradation of the protein into individual zig-zag elements or smaller pieces is attained. The interaction between influenza virus particles and inhibitory mucoprotein consists of the attachment of a fiber molecule to the virus ...
Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for...
The fusion of lysosomes to phagosomes was observed under high voltage electron microscopy, in 4μm thick rat retinal sections with the aid of acid phosphatase cytochemistry. The study of thick sections facilitates the observation of the moment of fusion in stereo view from two tilted pictures. From this study, the contents of the lysosome pored into the phagosome through the orifice, shortly after the collision of the two organelles. The hydrolytic enzymes such as acid phosphatase spread in a sheet under the limiting membrane of the phagosome to finally form a balloon of the reaction product. In some case the ballooning appeared to be doubled. The outer skin of the reaction product may be the result of a wrapping mechanism of phagolysosomes.. ...
Time-lapse images of particulate matter (PM) deposition on diesel particulate filters (DPFs) at the PM-particle scale were obtained via field-emission scanning electron microscopy (FE-SEM). This particle scale time-series visualization showed the detailed processes of PM accumulation inside the DPF. First, PM introduced into a micro-pore of the DPF wall was deposited onto the surface of SiC grains composing the DPF, where it formed dendritic structures. The dendrite structures were locally grown at the contracted flow area between the SiC grains by accumulation of PM, ultimately constructing a bridge and closing the porous channel. To investigate the dominant parameters governing bridge formation, the filtration efficiency by Brownian diffusion and by interception obtained using theoretical filtration efficiency analysis of a spherical collector model were compared with the visualization results. The initial deposition of PM on the SiC grains showed good agreement with theoretical observations, ...
Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.
A light-and electron-microscopic study of pig hepatocytes from late prenatal to early neonatal animals shows changes which reflect an increasing rate of synthetic activity. The granular endoplasmic reticulum (ER) in the prenatal pig hepatocyte is situated along the periphery of the cytoplasm and in the region immediately surrounding the nucleus. Mitochondria are most abundant in the area adjacent to the nucleus, while the Golgi complex is generally located in the region of the bile canaliculus. The remaining portion of the hepatocyte is occupied with glycogen. A few hours after birth the hepatocyte increases about twofold in size with the nucleus shifting from a peripheral to a more centrally located position. The glycogen decreases quickly coincident with a rapid increase in the amount of granular ER and the dispersion of the mitochondria throughout the cell. The Golgi complex becomes distended and numerous vesicles appear in its immediate vicinity containing a moderately dense material. ...
Hi, I am Stefan Fischer. I am biologist at the University of Tübingen, working in the department for Evolutionary Biology of Invertebrates (Institute of Evolution and Ecology). My methodological focus is set on electron microscopy. Furthermore I do focus on 3D techniques, be it on basis of semi-thin sections (LM), Ultra-thin sections (ssTEM) and also MicroCT data. For that reason I developed a strong interest in open source software packages and image analysis in order to automate (or semi-automate) some of the tedious parts of my workflows.. ...
Conventional electron microscopy offers a substantial resolution advantage over light microscopy, but requires difficult and often destructive preparation techniques. Recent advances in electron microscopy allow for imaging of hydrated samples, retaining the resolution advantage while removing the difficulty in preparation. Two new techniques, environmental scanning electron microscopy and wet electron microscopy offer this advantage, allowing for new possibilities in biological imaging.
The fine structure of the His bundle is described on the basis of its light and electron microscopic appearance. Electron microscopy was performed on one human and two canine hearts, and light microscopy on over 400 human and 60 canine hearts. The His bundle was identified by its light microscopic appearance. There were no significant differences in the fine structure of human and canine His bundles. In both, the principal cell was a typical Purkinje cell containing few myofibrils and a large perinuclear clear zone; these cells are shorter and broader than working myocardial cells, and their intercellular junctions (which are obliquely rather than transversely oriented) contain a high proportion of nexus formations. Both the human and canine His bundles are partitioned by fine collagen septa, which are longitudinally oriented with comparatively few crossover connections. The general organization of the His bundle is thus into multiple strands of Purkinje cells, and these strands are largely ...
With the use of the method described in the preceding paper (to be referred to subsequently as I) for constructing the displacement fields, the electron microscope image contrast of small dislocation loops and of stacking-fault tetrahedra has been computed from numerical solutions of the Howie-Whelan (1961) equations. The computer-simulated images, displayed in the form of half-tone pictures, have been used to identify the nature and geometry of such defects in ion-irradiated foils. A systematic study of the contrast of small Frank loops in Cu+ ion irradiated copper under a wide variety of diffraction conditions is reported. In particular the variations of the contrast of loops edge-on and inclined to the electron beam with the operating Bragg reflexion, the thickness and inclination of the foil, depth of the defect in the foil and deviation from the Bragg-reflecting condition have been studied. Methods of obtaining useful information, such as the diameters of the loops, are suggested. The ...
A new type of Sr-containing sialon polytypoid phase with the structural formula SrSi10-xAl18+xN32-xOx (x approximate to l) has been found in the Sr-Si-Al-O-N system. The phase was characterised by X-ray powder diffraction (XRPD), and its structure was investigated by electron diffraction (ED) and high resolution electron microscopy (HREM). It is considerably disordered, but the average structure has a rhombohedral unit cell with a=5.335(5)approximate to root 3.a(AIN) and c= 79.1(1)Angstrom approximate to 30.c(AIN). The Sr atoms ave located in layers M-Sr-M, M=(Si/Al), at the origin of the unit cell with 12 X= (O,N) atoms around it, at distances of similar to 3 Angstrom, forming a cubo-octahedron. The X atoms that form a hexagon around the Sr atom in the ab plane are corner shared by M = (Si/Al) tetrahedra with opposite polarity in adjacent layers in which 2/3 of the tetrahedra are occupied. The M-Sr-M layers alternate with normally eight-layer-thick AIN type blocks, although the thickness of ...
SMITH, DJ and FREEMAN, LA and MCMAHON, RA and AHMED, H and PITT, MG and PETERS, TB (1984) CHARACTERIZATION OF SI-IMPLANTED AND ELECTRON-BEAM-ANNEALED SILICON-ON-SAPPHIRE USING HIGH-RESOLUTION ELECTRON-MICROSCOPY. J APPL PHYS, 56. pp. 2207-2212. ISSN 0021-8979. Full text not available from this repository ...
RICHMOND, Va., June 7 /PRNewswire-USNewswire/ -- National Environmental Testing Lab Now IDs Carbon Black with Electron-Microscopy.
Learn how your ultrastructural investigations can benefit from modern electron microscope techniques. Correlative microscopy provides you deeper insights about cell structure by combination of light and scanning electron microscopy methods.
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics & structural biology at present.Frank, Joachim is the author of Three-Dimensional Electron Microscopy Of Macromolecular Assemblies Visualization Of Biological Molecules In Their Native State, published 2006 under ISBN 9780195182187 and ISBN 0195182189. [read more] ...
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
With the resolution becoming sufficient to reveal individual atoms, HREM is now entering the stage where it can compete with X-ray methods to quantitatively determine atomic structures of materials without much prior knowledge, but with the advantage of being applicable to aperiodic objects such as crystal defects. In our view the future electron microscope will be characterised by a large versatility in experimental settings under computer control such as the illumination conditions (TEM-STEM), CBED, detecting conditions (diffraction, image, ptychography) and many other tunable parameters such as focus (g), voltage, spherical aberration (C-s), beam tilt, etc. Since modem detectors can detect single electrons, also the counting statistics is known. The only limiting factor in the experiment will be the total number of electrons that interact with the object during the experiment due to the limitations in the exposure time or in the object damage. However, instrumental potentialities will never ...
Transmission electron micrographs showing the tracheal epithelium in Ig-deficient mice. The tracheal epithelium in SAL/SAL-treated animals (a) harbored only
A system for forming particulate material in a bulk form comprises a compression tower having a top and sides depending therefrom. A compression chamber having side walls and an open bottom is reciprocatively mounted to said tower for deposit of loose particulate material therein. The system further includes a first conveyor assembly displaced from said tower. In its extended position, the chamber contacts the first conveyor assembly and the bottom of the chamber is closed thereby. A vacuum blower draws loose particulate material into the chamber through an inlet and compresses it against the first conveyor assembly. A ram assembly within the chamber further compresses the loose material into a bulk form atop the conveyor belt. When the chamber and ram assembly are returned to their retracted positions, the material bulk is transferred downstream by the first conveyor assembly to a space between vertically spaced second and third conveyor assemblies.
Transmission electron micrograph of the cells of A. aeolicus.(A) Image of the whole cell and its flagellum. (B) and (C) Partial enlargements of the flagellated
Choosing a suitable particulate material for the material systems of the present invention involves various qualitative evaluations, which may easily be accomplished through routine experimentation by those of ordinary skill in the art. First, a small mound of particulate material is formed, a small depression is formed in the mound, and a small amount of fluid is placed in the depression. Visual observations are made regarding, among other things, the rate at which the fluid diffuses into the particulate material, the viscosity of the particulate material introduction of the fluid, and whether a membrane is formed around the fluid. Next, line testing is performed by filling a syringe filled with fluid and strafing the mounds of particulate material. After a period of about 24 hours, the mounds of particulate material are examined. Those in which pebbles of particulate material have formed are most suitable, as it means that the particulate material and fluid react more quickly than the fluid ...
Natural chalcopyrite (CuFeS2) specimens from Golden, New Mexico and Transvaal, South Africa were examined by transmission electron microscopy. The defect structure was composed of dislocations,...
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art.
A recording element is disclosed which is capable of providing high-quality, high information-density recording by thermal deformation. The element comprises a support having thereon a layer of amorphous material. The amorphous material is capable of being thermally deformed to form depressions surrounded by sharply defined ridges when impinged upon by a high energy-density recording beam. The amorphous material comprises either a binder and a dye or a certain solvent-coatable dye, Iosol Red. The amorphous material must have an absorption factor, as herein defined, greater than 20. The depressions which are formed in the described material are such that they are readable by a reading beam which is not absorbed by the material.
... (EMDB) is a public repository for electron microscopy density maps of macromolecular complexes and subcellular structures. It covers a variety of techniques, including single-particle analysis, electron tomography, and electron (2D) crystallography. The EMDB was founded at EBI in 2002, under the leadership of Kim Henrick. Since 2007 it has been operated jointly by the PDBe, and the Research Collaboratory for Structural Bioinformatics (RCSB PDB) as a part of EMDataBank which is funded by a joint NIH grant to PDBe, the RCSB and the National Center for Macromolecular Imaging (NCMI). ...
The electron microscope is relatively larger and uses a vacuum and electrons to produce a high quality image, by sending electrons through the tiny gaps. (An anode is there to speed it up). This gives an advantage to the electron microscope, a better resolution. As a result if any person wants to see something such as the mitochondrion, the Golgi Body or even a lysosome they are able to. It magnifies up to 200,000 times, which is a huge amount!. There are two types of electron microscope, the Scanning Electron Microscope [SEM] and the Transmission Electron Microscope [TEM]. Scanning Electron Microscope shows the external and whole image of a specimen such as a dust mite, or a nit. The Transmission Electron Microscope is literally the opposite. It gives the image from the interior. These are very exciting options as they give an overview of the entire specimen in question and we are able to study them in great detail and depth.. ...
Light and Electron Microscopic Evidence for a Direct Retinal Projection to the Pulvinar in the Asiatic Chipmunk,,I,Tamias sibricus,/I,. (1986 ...
Transmission electron microscopy was used to determine the structure of molecular films of self-assembled monolayers of pentathiophene derivatives supported on various electron transparent substrates. Despite the extreme beam sensitivity of the monolayers, structural crystallographic maps were obtained that revealed the nanoscale structure of the film. The image resolution is determined by the minimum beam diameter that the radiation hardness of the monolayer can support, which in our case is about 90 nm for a beam current of 5 × 106 e-/s. Electron diffraction patterns were collected while scanning a parallel electron beam over the film. These maps contain uncompromised information of the size, symmetry and orientation of the unit cell, orientation and structure of the domains, degree of crystallinity, and their variation on the micrometer scale, which are crucial to understand the electrical transport properties of the organic films. This information allowed us to track small changes in the ...
Virtual Nanoscopy: The Ridiculously Zoomable Cell... Color us impressed! A team of researchers... ...Our approach employs standard transmission electron microscopy , rapid automated data collection, and s... ...It greatly facilitates correlative light- electron microscopy studies to relate structure and functio... ...ues stitched together... http://www.neatorama.com/2012/08/08/Virtual-Nanoscopy-The-Ridiculously-Zoomable-Cell/ ...
Gadisseux, JF. ; Evrard, Philippe. Glial-neuronal Relationship in the Developing Central Nervous-system - a Histochemical Electron Microscope Study of Radial Glial-cell Particulate Glycogen in Normal and Reeler Mice and the Human-fetus. In: Developmental Neuroscience, Vol. 7, no. 1, p. 12-32 (1985 ...
Male chick embryos of a sex-linked cross were injected with 0.1 mg of estradiol benzoate on the fourth day of incubation. Their left gonads, together with ovaries from uninjected control females, were dissected at various intervals after hatching and processed for electron microscopic study. At the time of hatching no differences were found between the intersexual gonads of treated chicks and control ovaries with the exception of the presence, in the former, of great number of germ cells in interphase. Cortical degeneration in intersexual gonads began on the third day and was almost completed by the tenth. During this period germ cells underwent cytolysis while accompanying pre-follicular cells showed cytological characteristics which were undistinguishable from those found in control ovaries. This fact tends to suggest that the primary incompetency responsible for cortical degeneration lies in the germ cells and not in the pre-follicular cells.
As part of a project sponsored by The Ministry of Education, Culture, Sports, Science and Technology, Osaka Universitys Research Center for Ultra-High Voltage Electron Microscopy will hold a workshop -- Electronic Microscope School #2. This workshop is intended for in-house and outside researchers and for students in the second-term of doctoral programs that require microstructural analysis making use of an electronic microscope in the fields of life sciences and/or materials and device engineering.. First Day ...
Mutations in Trk-fused gene (TFG) have been linked to numerous forms of neurodegenerative disease. Using a combination of single particle electron microscopy approaches, small angle x-ray scattering, x-ray crystallography, and human stem cell models, we have determined the direct impacts of several of these mutations. In particular, mutations within the TFG amino-terminus alter the conformation of TFG ring complexes, which play an important role in the kinetics of secretory protein export from the endoplasmic reticulum (ER). Consistent with this idea, we find that cells harboring TFG mutations exhibit constitutively elevated levels of ER stress. Moreover, we find that neurons expressing these mutants fail to display normal axon bundling, suggesting that a deficit in protein export from the ER plays an important role during neurite outgrowth. ...
A multifunctional particulate material, fluid, or composition includes a predetermined amount of core particles with a plurality of coatings. The core particles have an average particle size of about 1 nm to 500 ~m. The particulate material, fluid, or composition is capable of exhibiting one or more properties, such as magnetic, thermal, optical, electrical, biological, chemical, lubrication, and rheological.
Our histological and ultrastructural study on the occurrence of cardiomyocytes in PVs of humans as well as of mice and rats showed major differences in cardiomyocyte distribution and localization. These data might be of considerable relevance in terms of understanding the development of AF in humans as well as on the choice of animal models of AF as discussed below.. In our study on mice and rats we found cardiomyocytes forming part of PV walls in all lungs under investigation. In mice, their occurrence and distribution was not related to a specific strain, being similar both in C57BL/6 mice and in BALBc-mice. Cardiomyocytes were found in vessels with diameters varying from 70 - 250µm. However, their occurrence had a random character, not being present in every vessel of the same size. At the hilus the presence of cardiomyocytes was a constant feature, whereas in vessels less than 70µm no cardiomyocytes were found. These data were in accordance with the lacZ expression in transgenic ...
Definition: applying immunohistochemistry or immunocytochemistry method to localize antigens or proteins at sub-cellular level using an electron microscope.. General Methods & Techniques. Antigen Retrieval Methods. Multiple Labeling Methods. Antigen-Antibody Specific Applications. Tissue-Cell Specific Applications. Virological Applications. Tumor, Disease & Diagnostic Applications. TEM Immunogold Labeling Protocol - Pre-embedding Method (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. TEM Immunogold Labeling Protocol - Post-embedding Method-1 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. TEM Immunogold Labeling Protocol - Post-embedding Method-2 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. ...
Dr. Cardona will present and discuss the complete neural circuits of a glomerularly organized olfactory system (the antennal lobe) and a parallel-fiber system for learning and memory (the mushroom bodies) as reconstructed from serial section electron microscopy in the larval Drosophila ...
View details for this PhD Studentship: Understanding Battery Chemistry with In-situ Electron Microscopy job vacancy at University of Oxford in...
The presence of a free surface between two fluids of different properties is a source of (geometric) nonlinearity due to the role of interface curvature on the normal stress balance when surface tension is present. Thus, even for linear bulk equations (Stokes flow), a rich solution structure can be found. One difficulty in solving such problems is that the free surface and the fluid domain(s) can change dramatically with time, or under variation of parameters. We have developed numerical methods to solve such problems using an ALE-based finite-element method. The deforming fluid domain is treated as a pseudo-elastic solid for small deformations, but can also be completely remeshed to handle extreme changes in geometry. Techniques for the continuation of solution branches in the presence of remeshing will be described and used to demonstrate the existence of new solutions for the canonical problems of viscous fluid flow on the outside or inside of rotating cylinders, and to quantify the accuracy ...
The Monash Centre for Electron Microscopy (MCEM) is a central Monash University Technology Research Platform. It provides a world-class capability in electron microscopy to enable researchers to solve major scientific challenges. MCEM conducts innovative research in electron microscopy, and provides advanced instrumentation, expertise and training to researchers from across the university, government and industry sectors. A brochure provides a small snapshot of just some of the resulting research impact. MCEM together with the Monash Ramaciotti Centre for Cryo-Electron Microscopy form the Victorian node of Microscopy Australia (formerly AMMRF). For further information about MCEM please download the MCEM User Manual.. ...
An interesting discovery by researchers with be published in Neuroscience regarding the hair cells of the inner ear. Using a high voltage electron microscope, the researchers determined that the rootlets of the hair cells continue through the cell to the striated organelle which is believed to be responsible for the cells stability. With the striated organelle connecting the rootlets to the cell membrane, this offers the opportunity of feedback from the cell to the very detectors that detect motion. One of the researchers states the following: "this suggests a new way to envision how hair cells work. Just as the brain adjusts the sensitivity of retinal cells in the eye to light, it may also modulate the sensitivity of hair cells in the inner ear to sound and head position". Feedback from the brain could be what changes the tension on the rootlets of the hair cells and their sensitivity to stimuli ...
The effect of grain crushing during one dimensional compression and triaxial compression tests is investigated in a double-porosity granular material using the Discrete Element Method (DEM). Experimental tests results from the literature on a porous crushable soil (pumice sand) were simulated in 3-D using a large number of particles and without the use of agglomerates. The numerical simulations show the importance of incorporating internal porosity as a material characteristic. The variation of internal porosity with particle dimensions is of fundamental importance while describing the mechanical behavior of the material. The contact model parameters were calibrated using low confinement triaxial compression experimental tests while the particle failure criteria was calibrated using high pressure one dimensional compression tests. Particle strength size effect was assumed equal to that observed in other porous geomaterials and ultimately derived from the variation of internal porosity. The good ...
Monash Centre for Electron Microscopy (MCEM) Information The Monash Centre for Electron Microscopy (MCEM) is a central university Research Platform that: conducts research in electron microscopy, and provides advanced instrumentation, expertise and teaching in electron microscopy. The MCEM suite of advanced instrumentation can determine the composition, structure and bonding of materials down to the atomic scale.…
TEMPOS is a tripod, with two pieces of next-generation equipment devoted to excellence in TEM (CHROMATEM and NANOMAX), and a third pole to back up the development of other local nanoscience activities (NANOTEM). NANOMAX and CHROMATEM will push forward the limits in the study of individual nano-objects and nanomaterials, from their growth to the most local measurement of their physical properties, with innovative instrumentation. NANOTEM, in addition to providing support to the whole nanoscience community on the Campus will permit the necessary preliminary studies required by the other two microscopes. The objects of study of the TEMPOS project are innovative materials, nanostructures and architectures, including spintronics and oxitronics devices, metamaterials, semiconducting nanowires and quantum emitters, carbon nanotubes, functional oxides.. ...
TEMPOS is a tripod, with two pieces of next-generation equipment devoted to excellence in TEM (CHROMATEM and NANOMAX), and a third pole to back up the development of other local nanoscience activities (NANOTEM). NANOMAX and CHROMATEM will push forward the limits in the study of individual nano-objects and nanomaterials, from their growth to the most local measurement of their physical properties, with innovative instrumentation. NANOTEM, in addition to providing support to the whole nanoscience community on the Campus will permit the necessary preliminary studies required by the other two microscopes. The objects of study of the TEMPOS project are innovative materials, nanostructures and architectures, including spintronics and oxitronics devices, metamaterials, semiconducting nanowires and quantum emitters, carbon nanotubes, functional oxides.. ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
The Electron Microscopy Unit provides service and in-depth practical training in sample preparation and electron microscopy at beginning and advanced levels. Our facilities include basic instrumentation as well as cutting-edge technologies for Biological as well as the Materials Sciences applications. The staff members help scientists from different fields to carry out their own research using the units equipment, as well as providing microscopy services ...
Depolarization of neurons in three week-old rat hippocampal cultures promotes a rapid increase in the density of surface NMDA receptors (NR), accompanied by transient formation of nonsynaptic NMDA receptor clusters, or NR islands. Islands exhibit cytoplasmic dense material resembling that at postsynaptic densities (PSD), and contain typical PSD components including MAGUKS, GKAP, Shank, Homer and CaMKII detected by pre-embedding immunogold electron microscopy. In contrast to mature PSDs, islands contain more NMDA than AMPA receptors, and more SAP102 than PSD95, features shared with nascent PSDs in developing synapses. Islands do not appear to be exocytosed or endocytosed directly as preformed packages because neurons lacked intracellular vacuoles containing island-like structures. Islands form and disassemble upon depolarization of neurons on a time scale of 2-3 min, perhaps representing an initial stage in synaptogenesis.. Significance Statement: Islands of extrasynaptic NMDA receptors populate ...
The present studies showed POD presence in the salivary glands and consequently in the gelling saliva (the stylet sheath). In contrast, POD lack was found for salivary secretions diffusing from salivary sheat material. Moreover, no activity of POD was visible/occurred in the watery saliva. These results are pro et contra the POD findings for the salivary secretions of aphids, published up till now [1,8,11,13,14,15,16,17,29,30], and seem to be substantial evidence to distinguish real function of aphid POD which remains debatable and even controversial still. In r sum on aphid saliva Miles [15] asked Why aphids secrete their own POD and apparently increase the formation of toxic/or deterrent compounds, in particular quinines that may be/are known to be toxic to insects due to their ability to copolymerize with proteins. According to the obtained results the POD action/activity of the aphid salivary secretions is closed within the stylet sheath. This location may limite effectively the ...
In contrast, proteoglycan aggregates in cartilage, seen on electron microscopy as granulelike structures, were clearly smaller in the mutant mice than in the
We seek an individual who has demonstrated excellence in one (preferably both) of the following areas in materials science: (1) Functional ceramic/electronic materials including synthesis, device fabrication and materials physics of these systems (2) Advanced structural characterization techniques with emphasis on electron microscopy related techniques. Preference will be given to the overall originality and promise of the candidates work rather than to the sub-area of research. A successful candidate will hold a Ph.D. in a closely related discipline with some post-PhD research and teaching experience ...
Introduction to microscopy techniques and their use in biology. Fluorescence microscopy, dyes, their characterization and use. Histochemical, immunohistochemical and immunofluorescence. Microscopic methods for detection of cell organelles ( nucleus, nucleolus, endoplasmic reticulum, Golgi apparatus, mitochondria, cytoskeleton, lysosomes, vacuoles and other membrane structures). Microscopic detection cell function and apoptosis (programmed cell death). Detection ions, reactive oxygen and nitrogen. Nanoparticles and their use in microscopic techniques. ...
Early buyers will receive 10% customization on reports.. Sample preparation is one of the most important operations when it comes to analysis of a sample. This procedure consumes around 60% of the total workload of labs. There are various steps involved in sample preparation, and following these steps is mandatory as the sample preparation process might become complicated if not executed properly with utmost caution. Sample preparation market has highlighted greater adoption of sample preparation instruments that not only simplify the analytical tasks, but also save considerable amount of time. The major applications of electron microscopy & sample preparation are in the life sciences and material sciences domains. Increasing life science applications of electron microscope and R&D expenditure across varied regions around the world have resulted in the overall growth of the electron microscopy and sample preparation market.. Another major factor driving the global electron microscopy & sample ...
The results of this study illustrate the remarkable regenerative capacity of the skeletal muscle of young mice to ischemic injury. The muscles most affected by FAL were the soleus and gastrocnemius of the lower leg. There was also evidence of ischemic injury in the thigh, but to a lesser extent. During the first 3 days following surgery, there was microscopic evidence of muscle damage and a pronounced loss of function. By day 7, the histological evidence showed a substantial resolution of the injury, and contractile force had been essentially normalized. By 28 days the muscles microscopic appearance was largely restored to baseline. Consistent with these findings is the fact that of the 1,289 known transcripts we identified as differentially expressed between ligated and sham surgery controls, none were induced or repressed by the day 28 time point.. Because the gene expression results are too extensive to be discussed in a single paper, we present selective results from the data set that we ...
Weve written a great deal about ways of making so-called "bio-fuels," those ethanol, methanol and even diesel substitutes that avoid the high toxicity and environmental harm of fossil fuels. Often though, these substitutes require the diversion of foodstocks or the use of exotic catalysts and high energy inputs to trigger the appropriate mechanisms. Scientists as Stanford University may have found a way to use copper, though, to make ethanol without corn or other plants. Theyve "created a copper-based catalyst that produces large quantities of ethanol from carbon monoxide gas at room temperature." Matthew W. Kanan, Assistant Professor at Stanford, has been working toward this kind of biofuel production for many years. His University profile contains the following: "The ability to … ...
2002 - Czech Je krátce popsán nízkonapěťový elektronový mikroskop LV EM 5 vyráběný firmou Delong Instruments a jsou prezentovány první zkušenosti s přípravou biologických preparátů pro pozorování v tomto mikroskopu. Je to unikátní přístroj, který je schopen v lékařském a biologickém výzkumu nahradit vysokonapěťový TEM. The low voltage electron microscope LV EM 5 manufactured by Delong Instruments is described and methods of preparation of biological specimens for the observation in this microscope are briefly evaluated. LV EM 5 is a unique apparatus that could replace in medical and biological research laboratories high volatage transmission electron microscopes. Keywords: specimen preparation; low voltage; transmission electron microscopy; elektronový mikroskop; elektrické napětí malé Available at various institutes of the ASCR První zkušenosti s nízkonapěťovým elektronovým mikroskopem ...
2002 - Czech Je krátce popsán nízkonapěťový elektronový mikroskop LV EM 5 vyráběný firmou Delong Instruments a jsou prezentovány první zkušenosti s přípravou biologických preparátů pro pozorování v tomto mikroskopu. Je to unikátní přístroj, který je schopen v lékařském a biologickém výzkumu nahradit vysokonapěťový TEM. The low voltage electron microscope LV EM 5 manufactured by Delong Instruments is described and methods of preparation of biological specimens for the observation in this microscope are briefly evaluated. LV EM 5 is a unique apparatus that could replace in medical and biological research laboratories high volatage transmission electron microscopes. Keywords: specimen preparation; low voltage; transmission electron microscopy; elektronový mikroskop; elektrické napětí malé Available at various institutes of the ASCR První zkušenosti s nízkonapěťovým elektronovým mikroskopem ...
FEI Company, a leading provider of three-dimensional (3D) molecular, cellular and atomic-scale imaging systems, today announced the new Falcon™ Direct Electron Detector for its Titan™ and Tecnai™ transmission electron microscopes (TEMs). The Falcon is based upon direct electron detection that enables the acquisition of low-noise images of delicate biological samples and other beam-sensitive materials that require low electron dose interactions to prevent radiation damage of the material.
Abstract: Three-Dimensional Electron Microscopy (3D EM) has emerged as a powerful technique for structural characterization. Near-atomic resolution structures of macromolecular assemblies imaged as "single particles" (i.e. in the absence of a crystalline array) are now being obtained. Arguably one of 3D EMs most exciting prospects is its potential ability to visualize, at high resolution and under physiological conditions, the conformational flexibility central to the function of many macromolecules. I will discuss the technique and some of the challenges we face to make this prospect a reality and will illustrate this using examples from our work on the ATP-dependent chromatin remodeling complex RSC ...
Chris Guerin, VIB Microscopy Core, Flanders Institute for Biotechnology, Ghent, describes how the Microscopy facility supports the diverse work of this large institute. Hear how electron microscopy systems from Zeiss have enabled scientists to easily correlate 3D and electron microscopy techniques to study cells, tissues and small organisms in high resolution. Learn how this quantitative method of analysis enables entire molecular pathways to be traced.
Intimacy counselling. Find out more about our free, confidential counselling with medical specialists trained in intimacy, body image, sexual confidence and relationships. Available to all those facing cancer and their partners, including members of the LGBTQI community. ...
How do eukaryotic cells morph into and maintain a myriad of different cell shapes? In many cases, crossbridged bundles of polarized actin filaments provide the cytoskeletal scaffolding for this. We are interested in discovering the critical components required for filament bundling and the assembly principles cells use to generate shape. We use Drosophila as a model system because we can employ molecular and genetic tools to modify cytoskeleton assembly and confocal and electron microscopic techniques to evaluate the cell biological consequences in real time or at high resolution. In order to permit detection of cell shape changes with high sensitivity, we study cells that have an extreme shape - primarily the bristle cells that cover the fly. These are single cells that grow to elongate a beautiful curved extension that is 400 µm in length. We are interested in two specific questions. How are extremely long actin bundles constructed and what role does filament dynamics play in this process. ...
Materials Testing and Inspection: Scanning Electron Microscope analysis Cross Section Analysis of PCBs Charpy Impact Test Computed Radiography Hardness Testing Maintenance and Materials Testing Material Fatigue Testing Tensile Strength Test
Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain […]. The post Three-Dimensional Scanning Electron Microscopy for Biology appeared first on Bitesize Bio.. ...
High temperature materials are divided into two main categories-semi-crystalline and amorphous-based on their difference in molecular structure.. Amorphous high temperature resins have a randomly ordered molecular structure which does not have a sharp melt point; instead amorphous materials soften gradually as the temperature rises.. These materials change viscosity when heated, but seldom are as easy flowing as semi-crystalline materials. They are isotropic in flow, shrinking uniformly in the direction of flow and transverse to flow. As a result, amorphous materials typically exhibit lower mold shrinkage and less tendency to warp than the semi-crystalline materials.. Amorphous resins lose their strength quickly above their glass transition temperature (Tg).. RTP Company modifies the following amorphous thermoplastic resins to meet the particular demands of your high temperature application. ...
... Prof. Dr. HUDA AL_KHATEEB 2011-2012 Lec.1 The Circulatory System Aim :study the histology of the circulatory system Objectives: 1. Study the layers and the sub-layers of the heart 2. Study the impulse conducting system structure and ultra-structure 3. Study the types of blood vessels 4. Study the classification of the arteries 5. Study the sorting of veins 6. Study the light and electron microscopical features of the capillaries 7. Classification of capillaries according to their ultra structures 8. Throw light on the lymphatic vessel 4 The circulatory system is categorized as: 1. blood vascular system: Consists of: 1. Heart 2. Vessels: A. arteries B. capillaries C. veins 2. Lymph vascular system: Consists of: 1. lymph vessels 2. organs Circulatory system can be divided into: 1. Macro-vasculature (includes vessels with more than 0.1mm in diameter). These vessels are seen grossly. 2. Micro-vasculature (includes arterioles, capillaries and postcapillary venules). These vessels are seen ...
Biocenter Oulu Electron Microscopy Core Facility provides services and training in various electron microscopy techniques for the analysis of biological specimens.
Transmission electron microscope image of a thin section cut through the pancreas (mammalian). This image shows a capillary within the pancreatic tissue (acinar cells in this image). Note the abundance of rough endoplasmic reticulum in the acinar cells. There is a red blood cell within the capillary. The capillary lining consists of long, thin endothelial cells, connected by tight junctions. The image shows fenestration of these endothelial cells. The image also shows synaptic vesicles in the neuron (nerve cell) next to the capillary. JEOL ...
The materials research laboratories within the EMC will be home to the brand new multimillion-pound Advanced Imaging of Materials (AIM) facility. Equipment includes a suite of high resolution scanning electron microscopes, a focussed ion beam SEM, a correlated optical microscopy suite, TEM and X-Ray imaging suite.. A scanning electron microscope (SEM) produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals (both electrons and photons) which contain information about the samples surface topography and composition.. The high resolution SEMs in the facility will allow observation of fine detail and can image and analyse materials at the nanometer scale.. The focussed Ion beam scanning electron microscope (FIBSEM) takes this one step further by allowing these features to be viewed in three dimensions at the same high resolution. This is achieved by simultaneously imaging and removing material with a ...
Welcome to the pages of the BioEM Lab of the University of Basel. The BioEM Lab offers electron microscopy (EM) structural investigations in the life sciences field to its customers. For tasks with a materials sciences focus, we recommend the Nano Imaging Lab instead, which is an electron microscopy center embedded in the Swiss Nanosciences Institute.. The BioEM Lab offers various life sciences high-resolution electron microscopy imaging methods, including cellular EM such as TEM imaging or volume reconstructions of tissue or tissue sections, or molecular EM such as high-resolution cryo-EM 3D reconstructions of biological macromolecules. Details about the methods offered can be found below.. ...
Transmission electron microscope image of a thin section cut through an area of mammalian pancreatic tissue. Image is a pancreatic acinar cell, showing nuclear pores and rough endoplasmic reticulum. JEOL 100CX ...
Seminar - Atomic Resolution Transmission Electron Microscopy experiments on single radiation sensitive nanoparticles November 16th 2017
Advances in scanning electron microscopy are taking this technology to the next level, driving research and opening new and exciting fields of application.
Influenza virus: Negative-stained transmission electron micrograph (TEM) depicting the ultrastructural details of a number of influenza virus particles, or "virions".Courtesy pingnews.comEvery time flu season comes around there seems to be concern whether the current flu shot vaccine will be able to stave off the viral attack. Thats because viruses and bacteria have a habit of mutating, which subsequently makes a particular vaccine against them ineffective. So each year the medical profession comes up with a vaccine they think will be an effective counterattack against the invading virus. Our own natural antibodies do something similar.. "Weve known for a long time that our antibody-forming system adapts itself to every microbe we encounter," said Gerald Weissmann, M.D. Hes Editor-in-Chief of the FASEB Journal where a new study on the subject appears. "What we didnt understand fully is exactly how this happens," he added.. When viruses or other microbes attack us, our bodys defense system ...
Electron microscopy (EM) is a highly specialized service offered by only a few laboratories in the US, including the MSU VDL. It allows the pathologist to examine the ultrastructure of cells and pathogens at magnifications up to 450,000 fold or more.. EM is commonly used to identify pathogens (especially novel ones) such as viruses, bacteria, fungi, parasites, and microsporidia. EM also helps to identify and characterize neoplastic cells and define cellular degenerative processes (particularly neurologic and renal diseases). For instance, many glomerulopathies cannot be identified without the benefit of EM. Samples can be submitted formalin fixed; however, we prefer to work with glutaraldehyde if especially high-quality images are needed. Small samples (,2-3 mm in diameter) are ideal, but larger samples (up to 3 cm) may be submitted and they will be trimmed in our laboratory. We can also work with previously paraffin-embedded tissue at an additional cost; however, diagnostic success may be ...
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Principles, operation, and techniques of transmission electron microscopy; acquiring and interpreting imaging, diffraction, and spectroscopy information; and hands-on experience with a transmission electron microscope.. ...
A new method for detecting abnormalities in unborn children is providing physicians with more information to analyze the results than conventional, microscopic testing, according to two George Washington University researchers.
Jointly founded by Forschungszentrum Jülich and RWTH Aachen University, ER-C operates high-performance electron microscopes permitting unique insights into the world of atoms. The major issues tackled by ER-C include the further methodological development of high-resolution electron microscopic and spectroscopic methods and their application, currently predominantly for materials research and nanotechnology. In the near future, the life sciences will be added as the first new field of application. As a national user facility for the scientific community, we enable researchers from science and industry to use our instruments and methods, supported by our experts.. ...
As a forum for professional feedback, submissions of letters are open to all. You do not need to be a subscriber. To avoid redundancy, we urge you to read other peoples letters before submitting your own. Name, current appointment, place of work, and email address are required to send a letter, and will be published with your review. We also require that you declare any competing financial interests. Unprofessional submissions will not be considered or responded to.. ...
Development of new procedures for understanding material properties on the atomic level with a special focus on ultrahigh resolution electron microscopy techniques. In the near future, the life sciences will be added as the first new field of application. Furthermore, we enable researchers from science and industry to use our instruments and methods, supported by our experts, and aim to establish a national user facility for the scientific community.. ...
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We review the use of restoration methods that recover the complex specimen exit wave from a suitably conditioned data set of high resolution transmission electron microscope images. Various levels of...
Drifting in and out of consciousness in that dark room seemed far superior to being up and about, feeling the pain that seemed to emanate from my chest and flow throughout my body. It was as if my blood had been replaced by some impossibly dense material that could barely flow, making every step I took an effort. If I didnt lie on that floor and feel my body supported all the way from that flat part of my skull to the small of my back, to the backs of my ankles, it felt like I might fall through to the center of the earth, through the dirt, rock, and then to the molten core where I would be incinerated. I wondered if that might be better than living like this. ...
It has been found that the enzymatic digestion products of proteins cause a rapid proliferation of blood monocytes in vitro. Digestion mixtures having anywhere from 12 to 68 per cent of their nitrogen in the amino form possess this property.. For continued proliferation, heparin plasma or serum must be repeatedly supplied with the digests. Without them, multiplication continues for only a few days, the coagulum in which the cells are embedded liquefies around the central fragment, and the cells disintegrate.. The enzymatic digests exert a marked effect on the morphological appearance of the cells and finally cause them to agglutinate. The extent of this effect is determined by the degree of hydrolysis of the digest, its concentration, and the length of time the cells are cultivated in it. The morphological appearance of the cells is also somewhat influenced by the nature of the plasma or serum used with the digest.. Digests having very little free amino nitrogen produce short, round, granular ...
Granular materials are large amorphous aggregates of discrete, individually solid particles. Despite seemingly simple ingredients, such aggregates exhibit a wide range of complex behaviours that defy categorization as ordinary solids or liquids. This includes non-Newtonian flow behaviour and collective jamming transitions. One of the key issues has long been how to link particle-level properties in a predictive manner to the behaviour of the aggregate as a whole. However, for actually designing a granular material, an inverse problem needs to be solved: for a given desired overall response, the task becomes finding the appropriate particle-level properties. This master class discusses new approaches to tackle the inverse problem by bringing concepts from artificial evolution to materials design. These results have general applicability and open up wide-ranging opportunities for materials optimization and discovery.
DD15.02 - High Resolution Scanning Transmission Electron Microscopy Shows MnMn/O Termination at the Co2MnSi/MgO Interface in a Magnetic Tunnel Junction ...
Abstract Transmission electron microscopy (TEM) is a unique tool to visualize polymer morphology: an intimate organization of the matter, encompassing crystalline features in semicrystalline po ...
Scanning Electron Microscopy (SEM) Service SEM and EDS Scanning Electron Microscopy (SEM) is an important tool for materials and failure analysis...
Barry J.C. and Anstis G.R. (1994) High resolution transmission electron microscopy. Metals forum, 18 1-2: 31-50. doi:10.1016/j.micromeso.2005.03.006 ...