Background and aims: Probe-based confocal laser endomicroscopy (pCLE) is used to differentiate between neoplastic and non-neoplastic colorectal polyps during colonoscopy. We aimed to assess the accuracy of two endoscopists starting to use real-time pCLE for differentiation of colorectal polyps and to determine the negative predictive value (NPV) for neoplasia in ... read more polyps ≤ 5 mm. Methods: Patients undergoing colonoscopy in a tertiary hospital were included in this prospective trial. After a training session, two colonoscopists assessed 50 polyps between August 2012 and April 2014. They sequentially used narrow-band imaging (NBI) and real-time pCLE to differentiate non-adenomatous, adenomatous, and carcinomatous polyps during colonoscopy. Histologic diagnosis by a gastrointestinal pathologist was the gold standard. Results were compared to post-hoc pCLE by a panel of gastroenterologists and pathologists. Results: The accuracy of real-time pCLE was 76 %, compared to 73 % for NBI, and ...
TY - JOUR. T1 - Probe-based confocal laser endomicroscopy (pCLE) images of submucosal growth of a duodenal mucous neck cell adenoma. AU - Tahara, Tomomitsu. AU - Horiguchi, Noriyuki. AU - Nagasaka, Mitsuo. AU - Nakagawa, Yoshihito. AU - Tsukamoto, Tetsuya. AU - Shibata, Tomoyuki. AU - Ohmiya, Naoki. PY - 2016/1/1. Y1 - 2016/1/1. UR - http://www.scopus.com/inward/record.url?scp=84955452020&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84955452020&partnerID=8YFLogxK. U2 - 10.1055/s-0041-111031. DO - 10.1055/s-0041-111031. M3 - Comment/debate. C2 - 26800196. AN - SCOPUS:84955452020. VL - 48. SP - E19-E21. JO - Endoscopy. JF - Endoscopy. SN - 0013-726X. ER - ...
TY - JOUR. T1 - Pre- and post-training session evaluation for interobserver agreement and diagnostic accuracy of probe-based confocal laser endomicroscopy for biliary strictures. AU - Talreja, Jayant P.. AU - Turner, Brian G.. AU - Gress, Frank G.. AU - Ho, Sammy. AU - Sarkaria, Savreet. AU - Paddu, Naveen. AU - Natov, Nikola. AU - Bharmal, Sheila. AU - Gaidhane, Monica. AU - Sethi, Amrita. AU - Kahaleh, Michel. PY - 2014/7. Y1 - 2014/7. N2 - Background and Aim Current diagnostic modalities for indeterminate biliary strictures offer low accuracy. Probe-based confocal laser endomicroscopy (pCLE) permits microscopic assessment of mucosal structures by obtaining real-time high-resolution images of the mucosal layers of the gastrointestinal tract. Previously, an interobserver study demonstrated poor to fair agreement even among experienced confocal endomicroscopy operators. Our objective was to assess interobserver agreement and diagnostic accuracy upon completion of a pCLE training session. Methods ...
New approaches to the chemotherapy of glioblastoma [Elektronische Ressource] : investigations on doxorubicin nanoparticles, inhibition of PDGF receptors and kinesin Eg5, with emphasis on confocal laser-scanning microscopy / vorgelegt von Dietmar Gross : New Approaches to the Chemotherapy of Glioblastoma: investigations on doxorubicin nanoparticles, inhibition of PDGF receptors and kinesin Eg5, with emphasis on confocal laser-scanning microscopy Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) der Naturwissenschaftlichen Fakultät IV - Chemie und Pharmazie - der Universität Regensburg
A Wavelet-Based Approach to 3-D Confocal Microscopy Image Reconstruction , A Wavelet-Based Approach to 3-D Confocal Microscopy Image Reconstruction , کتابخانه دیجیتال دانشگاه آزاد اسلامی خوراسگان
INTRODUCTION: Endoscopic evaluation with high-definition white light endoscopy and random 4-quadrant biopsy (Seattle Protocol) is the current standard of care for the detection of Barretts esophagus (BE). Recently, enhanced imaging technologies have become available to provide real-time diagnosis of intestinal metaplasia (IM) and dysplasia, reducing the need for tissue biopsy. Probe-based confocal laser endomicroscopy (pCLE) provides dynamic microscopic mucosal views, rapidly capturing digital images that become optical biopsies. This study examined the role of pCLE in BE screening and surveillance as compared to the Seattle Protocol.. METHODS: Patients undergoing BE screening or surveillance endoscopy were enrolled at eight US centers. Optical biopsy using pCLE was interpreted in real time. Endoscopists performing pCLE were new users with a median experience of 8.5 months and no formal training in surgical pathology. Seattle Protocol biopsies were then taken. Recorded pCLE images were reviewed ...
TY - JOUR. T1 - First experiences using reflectance confocal microscopy on equivocal skin lesions in Queensland. AU - Curchin, Claudia E S. AU - Wurm, Elisabeth M T. AU - Lambie, Duncan L J. AU - Longo, Caterina. AU - Pellacani, Giovanni. AU - Soyer, H. Peter. PY - 2011/5. Y1 - 2011/5. N2 - Background/Objectives: Reflectance confocal microscopy (RCM) is a non-invasive method of imaging human skin in vivo. The purpose of this study was to observe the experience of using RCM on equivocal skin lesions in a tertiary clinical setting in Queensland. Methods: Fifty equivocal lesions on 42 patients were imaged using a reflectance confocal microscope immediately prior to being excised. The images were then analysed blind to the histopathological diagnosis. The experience and problems encountered when using RCM on skin lesions for the first time was also observed. Results: On RCM analysis 12/13 melanomas (92.3% sensitivity, 75% specificity), 19/22 benign naevi (86% sensitivity, 95% specificity), 6/9 basal ...
Representative confocal microscopy images of cells exposed to green fluorescent nano-particles (40 nm in diameter). (A-C) Dural epithelial cells. (D-F) Dura
A prospective randomized study of 26 patients treated with flex in one eye, and smile in the other at the Department of Ophthalmology, Aarhus University Hospital. Preoperative spherical equivalent refraction averaged -7.50 D (range -6.00 to -9.75 D) in both groups. All patients had stable myopia and no other ocular diseases. A VisuMax® femtosecond laser (Carl Zeiss Meditec, Jena, Germany) was used. Lenticule diameters were the same in both eyes and ranged from 6.00 to 6.50 mm. Flap thickness was 110 to 120 ṁm, and flap/cap diameter ranged from 7.3 to 8.0 mm. Images of the subbasal corneal nerves were acquired by continuous through-focusing of the cornea using in vivo confocal laser-scanning microscopy (Heidelberg Engineering, Heidelberg, Germany). The detachable objective, the so called Rostock cornea module (RCM) , was used to optimize image acquisition. The NeuronJ Java program (http://www.imagescience.org/meijering/software/neuronj) was used to trace and quantify subbasal nerves. Mean ...
The pUL97 protein kinase encoded by human cytomegalovirus is a multifunctional determinant of the efficiency of viral replication and phosphorylates viral as well as cellular substrate proteins. Here, we report that pUL97 is expressed in two isoforms with molecular masses of approximately 90 and 100 kDa. ORF UL97 comprises an unusual coding strategy in that five in-frame ATG start codons are contained within the N-terminal 157 aa. Site-directed mutagenesis, transient expression of point and deletion mutants and proteomic analyses accumulated evidence that the formation of the large and small isoforms result from alternative initiation of translation, with the start points being at amino acids 1 and 74, respectively. In vitro kinase assays demonstrated that catalytic activity, in terms of autophosphorylation and histone substrate phosphorylation, was indistinguishable for the two isoforms. An analysis of the intracellular distribution of pUL97 by confocal laser-scanning microscopy demonstrated that both
Glomeruli are anatomical and possibly functional modules in the vertebrate olfactory bulb. We investigated the spatial arrangement of glomeruli in the olfactory bulbs of adult zebrafish (Brachydanio rerio). A solution of the lipophilic tracer Dil was injected into the nasal cavities. Axons of sensory neurons projecting from the olfactory epithelium into the bulb were traced anterogradely, thus labeling the whole population of glomeruli. The glomerular distribution was analyzed in detail by confocal laser-scanning microscopy. We find that a typical olfactory bulb contains a small number of about 80 glomeruli that have a stereotyped configuration in all animals investigated. All glomeruli exhibit bilateral symmetry. Twenty-two single glomeruli could be identified from animal to animal by their characteristic position and morphology. The remaining glomeruli either are embedded in glomerular plexus and therefore cannot be delineated reliably, or belong to a densely clustered subpopulation of on ...
Cell culture and toxicity studies. Human embryonic kidney cells (HEK293) were cultivated in DMEM supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2. Primary neuron cultures were prepared from hippocampi or cortex of C57BL/6 mice and cultured in neurobasal medium supplemented with B-27, N-2, and L-glutamine. All reagents were supplied by Life Technologies (Thermo Fisher Scientific). Neurons were cultivated at different densities depending on which culture plate was used (30,000/well for 96-well plates, 75,000/well for 24-well plates, and 900,000/well for 6-well plates). For confocal laser-scanning microscopy, neurons and HEK293 cells were cultivated on poly-L-lysine-coated and collagenized coverslips, respectively. Unless otherwise stated, all neuronal studies were conducted after 9 to 10 days in vitro (DIV).. Toxicity studies using the MTT assay were conducted in 96-well plates for 12 hours in the presence of 200 μM of each investigated metabolite, unless otherwise stated. For ...
Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.
The enamel defects (EDs) may present with a variety of clinical manifestations with increasing severity from the sole appearance of pale discoloration to remarkable structural alterations. EDs are responsible for higher caries receptivity. In vivo reflectance confocal microscopy (RCM) allows to image in vivo at microscopic resolution of the dental surface, thus avoiding the tooth extraction and the sample preparation because of its ability to optically scan living tissues along their depth. Aim of this study is the in vivo assessment at microscopic resolution of dental surfaces affected by EDs without resorting to invasive methods such as teeth extractions, to define histological findings occurring in chromatic and/or structural EDs. For the purpose, 15 children, referring at the Dental Clinic of the Second University of Naples, affected by several degrees of EDs, were enrolled and underwent in vivo RCM imaging to microscopically define the ED confocal features using a commercially available ...
Buchner AM, Gomez V, Heckman MG, Shahid MW, Achem S, Gill KR, Jamil LH, Kahaleh M, Lo SK, Picco M, Riegert-Johnson D, Raimondo M, Sciemeca D, Wolfsen H, Woodward T, Wallace MB. The learning curve of in vivo probe-based confocal laser endomicroscopy for prediction of colorectal neoplasia. Gastrointest Endosc. 2011 Mar; 73(3):556-60 ...
Plants are valuable systems for analyzing the acentriolar mitotic spindle. We have developed methods for imaging the mitotic spindle in living tobacco (Nicotiana tabacum) suspension culture cells expressing GFP-?-tubulin. The methods allow the spindle to be observed in living cells at high spatial and temporal resolution and rely on water immersion objectives, spinning disk optics, and high-sensitivity cameras. Here, we describe these methods and provide step-by-step protocols for certain key steps. We also describe a method for application and removal of inhibitors ...
Breast cancer is the most prevalent and deadly cancer among women worldwide. The current standard for breast lesion diagnosis is histologic assessment with hematoxylin and eosin (H&E) staining. Histology has high diagnostic accuracy, but requires extensive time and resources to perform. The objective of this work was to improve diagnosis of early breast cancers by developing approaches to rapidly image and characterize neoplastic tissue and the tumor microenvironment in high resolution optical images. Confocal fluorescence microscopy can image optical sections of tissue without the need for extensive tissue processing. Three studies were performed to evaluate if confocal microscopy images contain sufficient information to identify neoplasia in breast tissue. In a 31 patient study, five pathologists identified neoplasia with high accuracy in confocal and histologic images. In another study, an expert pathologist estimated tumor cellularity in core biopsies with moderate agreement between confocal ...
The use of blochemical fractionation, immunofluorescence laser-scanning confocal microscopy, and immunoelectron microscopy with mouse anti-human bcl-2 monoclonal antibody to analyze the subcellular localization of the bcl-2 gene product revealed the protein prominently in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial membranes. Electron microscopy at high magnification more precisely localized bcl-2 to the nuclear outer membrane as confirmed by the biochemical fractionation, as well as to mitochondrial outer and, to a lesser degree, inner membrane. This multisite membrane distribution of bcl-2 suggests an important role for this protein in several different membrane compartments.. ...
When T cells, B cells, and natural killer (NK) cells of the immune system interact with target cells, signaling molecules are accumulated in the plasma membrane at structures known as the immunological synapse. Evidence is accumulating that proteins, as well as signals, are transferred between the interacting cells at such contacts. NK cells receive inhibitory signals from cells that express self major histocompatibility complex (MHC) molecules on their surface. Earlier evidence had shown that NK cells can actually acquire MHC class I proteins during interactions with target cells. Now Vanherberghen et al. show that the exchange goes both ways and that NK receptors are transferred to cells that express MHC class I ligands. The authors monitored transfer of biotinylated killer Ig-like receptor (KIR) KIR2DL1 by immunoblotting or green fluorescent protein-tagged receptor by fluorescence-activated cell sorting or laser-scanning confocal microscopy and observed transfer of KIRs. The NK cell receptor ...
Areas of active investigation include: use of laser-scanning confocal microscopy to measure "calcium sparks", which are brief localized increases in fluorescence from a Ca-indicator such as fluo-3 that are thought to be reflective of the transient opening of one or a few RyRs (=ryanodine receptors), the Ca release channels of the sarcoplasmic reticulum (SR); the possibility that the mechanism of activation of RyRs involves both voltage-gating and Ca-gating; the nature of the mechanism whereby SR Ca release is inactivated by a rise in myoplasmic free [Ca]; the possibility that either activation or inactivation of SR Ca release may vary with the RyR isoform composition (RyR1, RyR3, etc.); estimation of local Ca movements within the sarcomere by means of computer modeling, including estimation of the kinetics of binding of Ca to the intracellular Ca buffers troponin, parvalbumin, ATP, and the SR Ca pump ...
Plant parasites secrete proteins known as effectors into host tissues to manipulate host cell structures and functions. One of the major goals in effector biology is to determine the host cell compartments and the protein complexes in which effectors accumulate. Here, we describe a five-step pipeline that we routinely use in our lab to achieve this goal, which consists of (1) Golden Gate assembly of pathogen effector-green fluorescent protein (GFP) fusions into binary vectors, (2) Agrobacterium-mediated heterologous protein expression in Nicotiana benthamiana leaf cells, (3) laser-scanning confocal microscopy assay, (4) anti-GFP coimmunoprecipitation-liquid chromatography-tandem mass spectrometry (coIP/MS) assay, and (5) anti-GFP western blotting ...
By enhancing the signal of genetically encoded markers expressed in defined circuit elements and quickly mapping them in large volumes of brain tissue, it enables sparse reconstructions of genetically defined circuit motifs. These motifs can be further used as road maps for targeted imaging of tissue subsets at high resolution, thus restricting imaging and segmentation time by enabling directed unsupervised reconstructions. As a proof of principle, this technique has been used to automatically reconstruct and visualize interneurons of different mouse retinas. This approach is not restricted to the retina and can be used to track long-range projections anywhere in the brain, e.g., from the retina to visual recipient layers located several millimeters away. Further, the same approach for unsupervised image processing can be applied to a variant of spectral confocal reflectance microscopy, facilitating the long range tracing of myelinated axons, as well as the automatic assessment of myelin ...
A variety of microscopic techniques have been utilized to study cyanobacterial associations with plant roots, but confocal laser scanning microscopy (CLSM) is the least used due to the unavailability of a suitable fluorescent dye. Commonly used lectins have problems with their binding ability with root cells and their visualization under CLSM. DTAF (5-(4,6-dichlorotriazinyl) aminofluorescein) is a fluorescent dye that has been widely used for staining various biological samples for fluorescent microscopy. It reacts with polysaccharides and peptides at ordinary conditions. The possible application and efficiency of DTAF for CLSM studies were examined in various aspects of cyanobacterial-plant interactions. Seedlings of Pisum sativum, Vigna rediata and Triticum aestivum were co-cultivated and stained with DTAF as a fluorochrome. Extracellular and intracellular interactions of cyanobacteria and the plant root surface were observed by CLSM. Results were compared with staining by other commonly used ...
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For these reasons, in vivo observation of the skin in its physiology and responses to stimuli has always been somewhat of a challenge. What Corcuff, P et al experimented with was a method of observing the skin of the forearm before sun exposure and for a month afterward using confocal microscopy techniques. Observations were made in regards to melanasome (melanin-containing organelle) caps within basal keratinocytes (skin cells in the deep epidermis). The use of in vivo confocal microscopy also "affords new insight to the role of melanin and its gradual migration after sun exposure" In vivo confocal microscopy involved reflected singals being used to create an image. Contrast was improved due to absorption and scattering (minimal loss of photons). Another feature that was regarded was realtime imaging to prevent blurring from motion caused by blood pulses and involuntary movement. ...
Multi-dimensional fluorescence imaging of live animal models demands strong optical sectioning, high spatial resolution, fast image acquisition, and minimal photobleaching. While conventional laser scanning microscopes are capable of deep penetration and sub-cellular resolution, they are generally too slow and causing excessive photobleaching for volumetric or time-lapse imaging. We demonstrate the performance of an augmented line-scan focal modulation microscope (aLSFMM), a high-speed imaging platform that affords above video-rate imaging speed by the use of line scanning. Exceptional background rejection is accomplished by combining a confocal slit with focal modulation. The image quality is further improved by merging the information from simultaneously acquired focal modulation and confocal images. Such a hybrid imaging scheme makes it possible to use very low power excitation light in high-speed imaging, and therefore leads to reduced photobleaching that is desirable for three-dimensional ...
Intra-cellular and inter-cellular protein translocation can be observed by microscopic imaging of tissue sections prepared immunohistochemically. A manual densitometric analysis is time-consuming, subjective and error-prone. An automated quantification is faster, more reproducible, and should yield results comparable to manual evaluation. The automated method presented here was developed on rat liver tissue sections to study the translocation of bile salt transport proteins in hepatocytes. For validation, the cholestatic liver state was compared to the normal biological state. An automated quantification method was developed to analyze the translocation of membrane proteins and evaluated in comparison to an established manual method. Firstly, regions of interest (membrane fragments) are identified in confocal microscopy images. Further, densitometric intensity profiles are extracted orthogonally to membrane fragments, following the direction from the plasma membrane to cytoplasm. Finally, several
Synthesis of a 1:1 mixture of lactose/mannose-QDs 20 b and lactose/maltotriose-QDs 21 b and confocal microscopy images after 2 h incubation with AS cells: A)
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function. synapse was substantially ...
Opterra II swept field scanning confocal microscope Brochure 2.8 MB Opterra II is the latest advancement in high-speed fluorescence microscopy designed specifically for live-cell microscopy. It uniquely combines the resolution of traditional confocal systems with the speed typically associated with wide-field imaging. ...
The Opterra II swept-field confocal microscope utilizes proprietary one-dimensional pinhole array technology to combine the resolution of traditional confocal systems with the speed typically associated with wide-field imaging. With its short acquisition times and cell-protecting minimization of photobleaching and phototoxicity, Opterra II is ideal for advanced live-cell imaging. ...
Line-scanning, with pupil engineering and the use of linear array detectors, may enable simple, small, and low-cost confocal microscopes for clinical imaging of human epithelial tissues. However, a fundamental understanding of line-scanning performance within the highly scattering and aberrating conditions of human tissue is necessary, to translate from benchtop instrumentation to clinical implementation. The results of a preliminary investigation for reflectance imaging in skin are reported.. © 2009 Optical Society of America. Full Article , PDF Article ...
HCS systems are basically fully automated microscopes: an automated stage moves the multi-well plate from position to position (according to a previously defined protocol) and at each position the same imaging procedure is performed. Dichroic mirrors and filters are automatically switched, the sample is illuminated at one or more excitation wavelengths and the images are recorded with a CCD camera. Optionally also brightfield images are recorded. A combination of a laser-based autofocus system and a software-based autofocus procedure ensure that the sample is always in focus during imaging. HCS systems are available in widefield configuration or as confocal setup as for certain experiments optical sections or 3D imaging is required. The confocal HCS systems usually employ a spinning disk technique to ensure fast image acquisition. For fluorescence excitation the systems are equipped either with Mercury or Xenon light sources or with lasers, which are frequently used in confocal HCS systems. When ...
In the MBS program, Barthel, who didnt take many molecular courses as an undergraduate, enrolled in a variety of molecular and cell biology, spectroscopy, cell physiology, and other courses.. The incredible access to University faculty and resources helped him get a paper published with Karen Mesce and several others about a new confocal microscopy technique used to image Golgi-stained neurons.. "It seems like everyone you work with around here is a genius. My knowledge base now has far surpassed what it was.". He also found a full-time job on campus at the Imaging Center, where he provided training, as well as imaged specimens such as intact and optically clear mouse brains in order to study viral induced genetic modification. (Imaging Center program director, Mark Sanders, was also a co-author of the paper and instrumental in Barthels success there.). "They have a lot of impressive high-end research equipment that they teach people how to use, in addition to providing sample preparation, ...
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Using the 3D spinning disk confocal microscope at CBST, we are able to observe this process in much greater detail (more than 10 times the useful information in terms of spatial and temporal resolution), and reconstruct video images to focus on the structure and behavior of the virological synapse, which forms at the interface of cells in contact. By uncovering the different modes of Gag movements in the donor cell, we may be able to identify new targets or strategies for antiviral therapy.. ...
MCF10A ductal carcinoma in situ cells are shown invading collectively through a fibrillar collagen gel and stained for F-actin (white) and DAPI (blue) and imaged using a spinning disk confocal microscope. Image © 2017 Jaquemet et al ...
D4832 - 16 Standard Test Method for Preparation and Testing of Controlled Low Strength Material (CLSM) Test Cylinders , backfill, CLSM, compressive strength, construction control, mix design, quality control, soil stabilization,,
Confocal microscopy is an optical imaging technique that scans a focused point of light around a sample to reconstruct an image in a point-by-point manner. A pinhole is used in the detection path to reject out-of-focus light and increase resolution and contrast compared to wide field illumination. The technique is particularly useful for three-dimensional imaging.. ...
The IXplore Spin microscope system utilizes an advanced spinning disk unit to provide a large field of view, fast 3D confocal imaging, and prolonged cell viability in time-lapse experiments.
Use laser scanning confocal microscopy to analyze only a slice of a sample, even a thick one. Thanks to this tutorial be able to make a comparison between confocal and widefield microscopy.
Whether you use spinning disk, SSD, NVMe, or a combination of each, the Cisco UCS S-Series has you covered to scale to Petabytes in just minutes.
By combing light sheet illumination with confocal microscopy, researchers were able to capture high resolution images of slices of whole mouse brains. The slices were reconstucted into a a macroscopic view of the whole organ with a resolutin of a few microns ...
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Materials Chemistry. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Materials Chemistry, Structural Chemistry. strukturkemi. ...
Endoscopy is a minimally invasive procedure used to screen patients and to investigate a wide variety of suspected health issues. It often involves
1. LeicaSP5/STED This microscope is an inverted fully motorized confocal microscope from Leica (SP5) combined with a super resolution module (Super Re...
Quantitative colocalization analysis is a tool to objectively estimate the degree of overlap (colocalization) of fluorescently labelled antigens in biological microscopy. It allows the drawing of unbiased conclusions about their interaction and function.
Magnified intelligence chromoendoscopy (FICE) plus probe-based confocal laser endomicroscopy (pCLE) for Gastric Intestinal Metaplasia (GIM) diagnosis: a feasibility trial. Research Question:. Is confocal endomicroscope feasible to diagnose gastric intestinal metaplasia?. Objective:. To evaluate the feasibility of confocal endomicroscope in diagnose gastric intestinal metaplasia.. Hypothesis:. Confocal endomicreosocpe can provide the accurate diagnosis of gastric intestinal metaplasia.. Research design:. Diagnostic study. Sample size:. The investigators follow the population in recent study from Imraporn et al.: Validity of magnify NBI for gastric intestinal metaplasia targeted biopsy (N= 50). Data analysis:. Confocal Barretts esophagus classification was used to evaluate agreement of confocal endomicroscopic finding in gastric intestinal metaplasia. The accuracy of new criteria for GIM by confocal endomicroscope was evaluated in relation to pathological report, a gold standard for diagnosis, ...
This study aims to investigate the roles of the Notch-Hes1 pathway in the advanced glycation end product (AGE)-mediated differentiation of neural stem cells (NSCs). We prepared pLentiLox3.7 lentiviral vectors that express short hairpin RNA (shRNA) against Notch1 and transfected it into NSCs. Cell differentiation was analyzed under confocal laser-scanning microscopy. The percentage of neurons and astrocytes was quantified by normalizing the total number of TUJ1+ (Neuron-specific class III β-tubulin) and GFAP+ (Glial fibrillary acidic protein) cells to the total number of Hoechst 33342-labeled cell nuclei. The protein and gene expression of Notch-Hes1 pathway components was examined via western blot analysis and real-time PCR. After 1 week of incubation, we found that AGE-bovine serum albumin (BSA) (400 μg/mL) induced the astrocytic differentiation of cultured neurospheres and inhibited neuronal formation. The expression of Notch-Hes1 pathway components was upregulated in the cells in the AGE-BSA
Clinical applications of corneal confocal microscopy Mitra Tavakoli1, Parwez Hossain2, Rayaz A Malik11Division of Cardiovascular Medicine, University of Manchester and Manchester Royal Infirmary, Manchester, UK; 2University of Southampton, Southampton Eye Unit, Southampton General Hospital, Southampton, UKAbstract: Corneal confocal microscopy is a novel clinical technique for the study of corneal cellular structure. It provides images which are comparable to in-vitro histochemical techniques delineating corneal epithelium, Bowman’s layer, stroma, Descemet’s membrane and the corneal endothelium. Because, corneal confocal microscopy is a non invasive technique for in vivo imaging of the living cornea it has huge clinical potential to investigate numerous corneal diseases. Thus far it has been used in the detection and management of pathologic and infectious conditions, corneal dystrophies and ecstasies, monitoring contact lens induced corneal changes and for pre and post surgical evaluation
Confocal laser endomicroscopy (CLE) is a new diagnostic technique that allows microscopic examination of the digestive mucosa during ongoing endoscopy. Different types of tissue and diseases can be diagnosed immediately, and analysis of the in vivo microarchitecture is helpful to better target standard biopsies and reduce the number of biopsies required. CLE necessitates an intravenous injection of a fluorescent marker, e.g. fluorescein, to obtain optical biopsies with a high level of magnification (up to 1000 fold). To date, more than 1000 endomicroscopy procedures have been performed in the world and different publications have shown the safety, feasibility and excellent diagnostic yield of CLE. No complication related to IV injection of fluorescein has been reported. However, all these data come from a very limited number of expert centres and need to be confirmed and validated at the multicenter level. The aims of this multicenter trial are: 1) to standardize CLE in all centres equipped in ...
Random biopsies with targeted biopsies of any suspicious lesions is a reasonable alternative if chromoendoscopy is not available or if the yield of chromoendoscopy is reduced by significant underlying inflammation, pseudopolyposis, or poor preparation (ASGE, 2015). While there is emerging evidence that chromoendoscopy may yield higher polyp detection rates, at this time it is not known if the additional polyps detected are clinically significant and whether a higher detection rate results in a meaningful clinical outcome benefit. Confocal Laser Endomicroscopy Confocal laser endomicroscopy (also known as confocal fluorescent endomicroscopy) is an endoscopic technique that makes it possible to carry out microscopic examination of a smaller, focused spot of the mucosal layer during endoscopic procedures. Rasmussen and colleagues (2015) conducted a systematic review of the current indications and perspectives of confocal laser endomicroscopy (CLE) for IBD. Only studies reporting original clinical ...
This well established symposium will cover various aspects of modern Raman microscopy and detail the advantages of confocal Raman imaging and its applications. Scientific talks from distinguished speakers in academia and industry, the contributed talk and poster sessions, and the instrument demonstration will give the participants a deeper understanding of confocal Raman imaging.
Scanning confocal laser microscopy (SCLM) was used to visualize fully hydrated microbial biofilms. The improved rejection of out-of-focus haze and the increased resolution of SCLM made it preferable to conventional phase microscopy for the analysis of living biofilms. The extent of image improvement was dependent on the characteristics of individual biofilms and was most apparent when films were dispersed in three dimensions, when they were thick, and when they contained a high number of cells. SCLM optical sections were amenable to quantitative computer-enhanced microscopy analyses, with minimal interference originating from overlying or underlying cell material. By using SCLM in conjunction with viable negative fluorescence staining techniques, horizontal (xy) and sagittal (xz) sections of intact biofilms of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Vibrio parahaemolyticus were obtained. These optical sections were then analyzed by image-processing techniques to assess the ...
TY - JOUR. T1 - Adaptations of diaphragm neuromuscular junction following inactivity. AU - Prakash, Y.s.. AU - Zhan, W. Z.. AU - Miyata, H.. AU - Sieck, Gary C. PY - 1995. Y1 - 1995. N2 - We hypothesized that differences exist in the morphological adaptations of neuromuscular junctions (NMJs) on different fiber types in response to prolonged inactivation. Two weeks of inactivity of both phrenic motoneurons and diaphragm muscle was induced by spinal cord hemitransection at C2 (spinal isolation; SI). A three-color fluorescent immunocytochemical technique, combined with laser-scanning confocal microscopy, was used to create two- (2D) and three-dimensional (3D) images of NMJs and obtain morphological information concerning: (1) innervating axons and presynaptic nerve terminals; (2) motor endplates (postsynaptic apparatus consisting of acetylcholine receptors), and (3) myosin heavy chain (MHC) phenotype of muscle fibers. In both sham controls (CTL) and SI animals, planar (2D) and surface (3D) areas ...
With the use of HRTII-RCM, we have obtained in vivo images of the central cornea in eyes affected by TSPK. Although dendritic cells were observed in the unaffected healthy corneas of the two patients with unilateral TSPK, these cells were greatly increased in number and formed aggregates in both the basal cell layer of the corneal epithelium and in Bowmans layer of the affected eyes. The dendritic cells in Bowmans layer appeared associated with the nerve fibers that form the nerve plexus of the corneal epithelium. Medical intervention reduced the numbers of these dendritic cells to normal values. With use of HRTII-RCM, we were able to observe abnormalities of cell shape and cell density in the eyes affected by TSPK. In the affected eyes, accumulation of dendritic cells was apparent in the focal lesions. Tandem scanning confocal microscopy of eyes affected by TSPK previously revealed multiple highly reflective filamentous structures in Bowmans layer of the corneal epithelium, but these ...
White identified the first gene with a demonstrated role in determining synaptic specificity. He studied the role of cell-cell interaction in determining the lineage pattern, stimulating a wide field of research. In more recent work, John and his co-workers partially confirmed his earlier model of cytokinesis; they discovered genes controlling cytokinesis and found features previously thought specific to plant cell division. Recognising the potentialities of laser-scanning confocal microscopy, John built a prototype microscope: with William Bradshaw Amos he developed this into a commercially produced instrument now widely used.[5] ...
Background and study aims: Confocal laser endomicroscopy (CLE) allows mucosal barrier defects along the intestinal epithelium to be visualized in vivo during endoscopy. Training in CLE interpretation can be achieved didactically or through self-directed learning. This study aimed to compare the effectiveness of expert-led didactic with self-directed audiovisual teaching for training inexperienced analysts on how to recognize mucosal barrier defects on endoscope-based CLE (eCLE). Materials and methods: This randomized controlled study involved trainee analysts who were taught how to recognize mucosal barrier defects on eCLE either didactically or through an audiovisual clip ...
Four types of confocal microscopes are commercially available: Confocal laser scanning microscopes use multiple mirrors (typically 2 or 3 scanning linearly along the x and the y axis) to scan the laser across the sample and "descan" the image across a fixed pinhole and detector.. Spinning-disk (Nipkow disk) confocal microscopes use a series of moving pinholes on a disc to scan spots of light. Since a series of pinholes scans an area in parallel each pinhole is allowed to hover over a specific area for a longer amount of time thereby reducing the excitation energy needed to illuminate a sample when compared to laser scanning microscopes. Decreased excitation energy reduces photo-toxicity and photo-bleaching of a sample often making it the preferred system for imaging live cells or organisms.. Microlens enhanced or dual spinning disk confocal microscopes work under the same principles as spinning-disk confocal microscopes except a second spinning disk containing micro-lenses is placed before the ...
The Advanced Light Microscopy Facility (ALMF) is available to all workgroups of the Institute of Biology of the Humboldt-University Berlin. Providing sufficient free capacity ALFM also provides access to its equipment for other groups of the Humboldt-University Berlin and for collaborating users not associated with the Humboldt-University of Berlin. We offer various methods of light microscopy, for instance epifluorescence microscopy, confocal laser scanning microscopy (CLSM) and spinning disk confocal laser microscopy (SDM). The instruments are equipped for the following advanced techniques: ...
Intravital microscopy of leukocyte-endothelial dynamics using the Heidelberg confocal laser microscope in scleritis and allergic conjunctivitis Journal Articles Refereed ...
Confocal Raman microscopy is a high-resolution imaging technique that is widely used for the characterization of materials and specimens in terms of thei...
Gastroenterology Research and Practice is a peer-reviewed, Open Access journal that provides a forum for researchers and clinicians working in the areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis, and therapy of gastrointestinal diseases.
Green fluorescent protein (GFP) is a molecular marker used in synthetic biology to report gene expression and successful transformation, and in fluorescence confocal microscopy to label or characterise proteins and pathways. Equipment used to measure its expression is usually present in most laboratories, making it an accessible marker to use. Fluorescein, a bright green fluorophore, and its derivatives for example Fluorescein Isothiocyanate (FITC), have high absorptivity, excellent fluorescence and good water solubility, characteristics making them the most common fluorescent reagents for biological research (ThermoFisher Scientific). Together, these markers can be used as powerful tools for synthetic biology measurement and analysis, especially when used in technical standards. Without such standards, it can be difficult to obtain reliable and repeatable results, as cells are inherently complex biological systems that give differing results depending on the methods and machinery used. Absolute ...
Damage to peripheral nerves caused during a surgical intervention often results in function loss. Fluorescence imaging has the potential to improve intraoperative identification and preservation of these structures. However, only very few nerve targeting agents are available. This study describes the in vivo nerve staining capabilities of locally administered fluorescent lectin-analogues. To this end WGA, PNA, PHA-L and LEL were functionalized with Cy5 (λex max 640 nm; λem max 680 nm). Transfer of these imaging agents along the sciatic nerve was evaluated in Thy1-YFP mice (n = 12) after intramuscular injection. Migration from the injection site was assessed in vivo using a laboratory fluorescence scanner and ex vivo via fluorescence confocal microscopy. All four lectins showed retrograde movement and staining of the epineurium with a signal-to-muscle ratio of around two. On average, the longest transfer distance was obtained with WGA-Cy5 (0.95 cm). Since WGA also gave minimal uptake in the lymphatic
AIM: Endothelial glycocalyx (eGLX), the inner lining of glomerular capillaries, regulates glomerular albumin permselectivity and is damaged in diabetes. Recently, it has been shown that the eGLX can be modified and repaired by different substances, in particular angiopoietinl (Angl) and hydrocortisone. We hypothesise that direct restoration of eGLX in the glomerulus will improve glomerular permeability in diabetes. To be able to test this hypothesis Angl and hydrocortisone were tested in vitro, then the best candidate was chosen for further ex vivo studies. To be able to test this compound ex vivo I also designed, characterised and validated a new albumin permeability assay based on quantitative fluorescence confocal microscopy. RESULTS: Angl was found to induce the most significant eGLX changes in vitro. Angl increased the expression of surface glycosaminoglycans (GAG) and significantly increased their release into the media of human conditionally immortalised glomerular endothelial cells ...
The Human Protein Atlas portal is an open-access database supplying protein and RNA expression data for postnatal human tissues and cell lines.. The protein expression data includes millions of high-resolution images showing the spatial distribution of proteins in 46 different postnatal normal human tissues, 20 different cancerous tissues, and 47 different human cell lines. For a large fraction of proteins, a results of a protein array assay and an immunofluorescence-based confocal microscopy image are also provided. In LifeMap database, only data from normal tissues are presented. Additionally, only genes with moderate to strong staining intensity and high confidence level are shown (described as supportive/high reliability; see website for further information about the calculation methods).. This experiment card only contains the protein expression data. See RNA expression data in Large Scale Dataset: Human Protein Atlas - RNA Sequencing Data.. ...
These are overlaid confocal microscopy images of E-cadherin, Actin and DAPI staining in fibronectin-depleted MCF-10A-Ca1h (Ca1h) breast cancer cells. In the article, beginning on page 1579, Shinde and colleagues demonstrate that wild type Ca1h cells are not metastatic, express high amounts of intracellular fibronectin, and display a very mesenchymal phenotype. In contrast, when fibronectin is depleted from the Ca1h cells they undergo a mesenchymal-epithelial transition (as shown) characterized by a return of junctional E-cadherin expression and an enhanced ability to form pulmonary tumors following tail vein injection. As opposed to the tumor promoting role of fibronectin in the extracellular matrix, this study suggests that when epithelial-derived carcinoma cells undergo epithelial-mesenchymal transition, constitutive expression of fibronectin stabilizes their mesenchymal phenotype and inhibits the cell autonomous ability of those cells to complete the final steps of the metastatic process. ...
Expression and localization of proteins in a large variety of normal human tissues, cancer cells and cell lines with the aid of immunohistochemistry (IHC) images and immunofluorescence (IF) confocal microscopy images.. ...
Characterisation of cornerstone adhesions. a Spinning disk images of hPSC plated on vitronectin (VTN) and stained for F-actin, paxillin, DAPI, and the pluripotency marker Nanog. Scale bar 10 μm. b Structured illumination microscopy images of hPSC plated on VTN and stained for filamentous actin (F-actin) and paxillin. Scale bar 10 μm. The white square highlights a region of interest (ROI), which is magnified. c-f Live-cell imaging of endogenously tagged paxillin in hPSC. Images were acquired every minute using a spinning disk microscope. Colonies were recorded for at least 105 min (n = 16 independent colonies from three biologically independent experiments). A representative video is available as supplementary information (Supplementary Movie 1). c A representative image is displayed (ROI highlighted by white square and magnified; size, 30 µm) in addition to a mask image highlighting paxillin-positive adhesions (colony edge FA, green; colony centre FA, magenta) and merged images depicting ...
a) Illustration of the orientation and the dimension of the x-z optical section transverse to the capillary in the mesentery. The mesentery is arranged horizontally on the surface of the coverslip that forms the base of the observation stage. The optical section is composed of multiple line scans of the laser along the x axis, collected as the objective is lowered along the z axis. (b) When the fluorescein-dyed α-lactalbumin is perfused into the vessel lumen, the dye will travel across the vessel wall and accumulate in the tissue space surrounding the vessel. (c) An x-z confocal image after 13s perfusion of FITC-α-lactalbumin. It shows that the fluorescence dye fills the microvessel lumen and spreads in the surrounding tissue [schematic in (b)]. ...
Glutamate induces de novo growth of functional spines in developing cortex Nature 474, 7349 (2011). doi:10.1038/nature09986 Authors: Hyung-Bae Kwon & Bernardo L. Sabatini. Mature cortical pyramidal neurons receive excitatory inputs onto small protrusions emanating from their dendrites called spines. Spines undergo activity-dependent remodelling, stabilization and pruning during development, and similar structural changes can be triggered by learning and changes in sensory experiences. However, the biochemical triggers and mechanisms of de novo spine formation in the developing brain and the functional significance of new spines to neuronal connectivity are largely unknown. Here we develop an approach to induce and monitor de novo spine formation in real time using combined two-photon laser-scanning microscopy and two-photon laser uncaging of glutamate. Our data demonstrate that, in mouse cortical layer 2/3 pyramidal neurons, glutamate is sufficient to trigger de novo spine growth from the ...
Hi all,I have a modified RO (reverse osmosis) membrane and I want to check biofilm growth on the membrane surface. For this purpose, Im using two bacterial strains (E.coli and Pseudomonas aeruginosa) in my system. I want to see and analyze (using confocal microscope) which strain is causing more biofilm on the membrane surface when grown in mixed culture. But, since Im not microbiologist I dont really know which stains I have to use. - Do I have to stain each of the bacteria before mixing and growing them in the system (e.g. red for E.coli and green for P.aeruginosa)? - Are the stains (e.g. SYTO 59) permanent stains? Thanks a lot for your help! Ruth ...
Cell Culture, Drug Treatment, and Cytotoxicity Assay. Human hepatoma HepG2 cells were purchased from American Type Culture Collection (Manassas, VA) and cultured according to a method described previously (Chen et al., 2000). Ketamine was dissolved in phosphate-buffered saline (0.14 M NaCl, 2.6 mM KCl, 8 mM Na2HPO4, and 1.5 mM KH2PO4). Concentrations of ketamine (≤100 μM), which correspond to clinical plasma concentrations (Domino et al., 1982; Grant et al., 1983), were chosen as the treated dosages in this study. To avoid drug interaction, when HepG2 cells were exposed to ketamine, the culture medium did not contain serum and antibiotics. Levels of γ-glutamyltranspeptidase and lactate dehydrogenase in the culture medium were measured to evaluate the cytotoxicity of ketamine to HepG2 cells as described previously (Wu et al., 2007).. Confocal Microscopic Analyses of F-Actin and Microtubular Cytoskeletons. The F-actin and microtubular cytoskeletons in HepG2 cells were visualized as described ...
Many new drugs are being developed that may slow PD progression. Currently, the main way to measure whether these drugs are working is to examine individuals at regular intervals but this examination varies greatly from one day to the next, such that it takes hundreds of participants over several years to find out if a new drug works. We hope to use a simple eye test to measure whether these drugs can slow nerve damage associated with PD. By using a more reliable and accurate measure, we hope to make an earlier diagnosis possible and to improve clinical trials to speed up the approval of new drugs.. Next Steps for Development: ...
A Quick Overview of Cancer Protocols and Upcoming Changes in Tumor Staging" by Julie Ann Walby, M.D.. "MUSE and Confocal Laser Endomicroscopy" by Luis Brandi, M.D.. ...
Precision Raman Equipment for Confocal Raman Spectroscopy. Find out about our Raman Spectroscopy Equipment, including Confocal Raman Microscope.
분광기는 기초과학인 물리학과 화학에서는 기본장비로 활용되지만 응용분야인 바이오,나노,환경,재료 등 시험평가 부분에서는 Raman(라만),Photo Luminescence(광발광),Fluorescence(형광발광)시스템으로 핵심적인 역할을 한다. 이에 따라 동우옵트론은 마이크로 라만(Micro Raman) 공촛점 라만(Confocal Raman) PL/Raman mapping 시스템을 개발,이를 국내외에 공급함으로써 여러분야 발전에 이바지 하고 있다.. ...
Raman spectroscopy has long been applied in geoscience, for example for the identification and characterization of minerals, or in the observation of mineral phase transitions in high and ultra-high pressure/temperature experiments. In most cases because measurements have been carried out in a micro-Raman set up very little detail on the spatial distribution and association components or mineral phases, or chemical variation could be observed. In this application note from WITec, by means of Con
The |i|Journal of Biomedical Optics|/i| (JBO) publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research.
The Zeiss LSM 710 Confocal Laser Scanning Microscope. Laboratories for Molecular Medicine, Room 111. The Zeiss LSM 710 confocal laser scanning microscope is designed for imaging fluorescence, either in fixed or live samples. A pinhole removes the out-of-focus light and allows investigators to acquire thin optical sections at various focal planes. Stacks of images can be acquired for 3D visualization.. The confocal module is mounted on a Zeiss Axio Observer Z1 inverted microscope with high quality objectives (listed below). A heated stage is available for imaging live samples at 37°C. The microscope has multiple lasers for excitation at 405, 458, 488, 514, 561, 594, and 633 nm, and is equipped with a 34-channel QUASAR detector, which can be set to collect emitted light of any wavelength within the visible spectrum. The QUASAR detector is capable of acquiring spectral information and its flexibility allows imaging of a broad range of fluorescent indicators, including the Alexa-Fluors, ...
Ex-Demonstration Microscope for Sale, Reduced Price. The Nikon Eclipse Ci-E is a laboratory grade microscope equipped with LED lighting, ceramic surface stage and polarisation attachments. An ergonomic tube capable of camera integration is provided. The motorised nosepiece and condenser make this microscope ideal for use by RSI sufferers or those working with constant and numerous magnification change. The Ci-E also features Auto Light Intensity Reproduction where the user defined light intensity for each objective is automatically memorised and replicated when the objective is used again. Four High end Plan Fluor (Semi-Apochromat) objectives 4x/10x/40x/100x oil complete the set. Additional objectives may be added and Epi-fluorescence may be attached. Read more online or contact us today.. The Ci-E will be on display on our booth at Pathology Update, at the Melbourne Convention centre 27 February - 1 March 2015. ...
The present study uses confocal laser scanning fluorescence microscopy to assess the penetration of protein hydrolyzates into hair. While higher amounts of protein were found in the cuticle, still significant quantities were observed in the cortical parts of hair, and this penetration was enhanced by longer incubation times.
OXFORD, UK -- Engineers at the University of Oxford have discovered a way to get up to 50 times the light efficiency from confocal microscope systems
Confocal microscopy with optical sectioning has revolutionized biological studies by providing sharper images than conventional optical microscopy. Here, we introduce a fluorescence imaging method with enhanced resolution and imaging contrast, which can be implemented using a commercial confocal microscope setup. This approach, called the reversibly switchable photo-imprint microscopy (rsPIM), is based on the switching dynamics of reversibly switchable fluorophores. When the fluorophores are switched from the bright (ON) state to the dark (OFF) state, their switching rate carries the information about the local excitation light intensity. In rsPIM, a polynomial function is used to fit the fluorescence signal decay during the transition. The extracted high-order coefficient highlights the signal contribution from the center of the excitation volume, and thus sharpens the resolution in all dimensions. In particular, out-of-focus signals are greatly blocked for large targets, and thus the image ...
or outsourced shared service solutions to increase the currently very low utilisation levels of PCs and servers and Growing volumes of data mean that decisions or lack of decisions about storage have considerable energy implications more rigorous information on life cycle management is needed to eliminate storage of data that are no longer required or to increase the proportion that utilises low energy storage rather than always on spinning disks There is enormous scope to create much more sustainable data centres through purchasing servers with lower power requirements increasing utilisation rates through consolidation and virtualisation and by changing physical aspects such as layouts cooling and power supply so that their energy overhead above that used by servers is only 20 30 compared to the current 40 100 In the medium term greater use of renewable or low carbon energy supply is feasible This could be facilitated by a move to shared service data centres if these enable more optimal choices ...
, treated with OSI-027 or rapamycin, and examined by confocal microscopy for autophagosome formation. As indicated in supplemental Figure 3A-B, OSI-027 and rapamycin increased autophagy to the same extent. In further experiments, cells were treated with OSI-027 in the absence and presence of 3-methyladenine or chloroquine, 2 reported autophagy inhibitors.37 As indicated in supplemental Figure 3C,
Researchers with Carnegie Mellons Molecular Biosensor and Imaging Center (MBIC), the Pittsburgh Supercomputing Center (PSC) and the University of Pittsburghs Center for Biological Imaging (CBI) will help to usher in an era of open data research in neuroscience by building a confocal fluorescence microscopy data repository. The data archive will give researchers easy, searchable access to petabytes of existing data.
For live cell and tissue imaging, sensitive high resolution and spectral imaging of wide range of fluorophores in fixed and living specimens.
GlobalSpec offers a variety of Microscopy for engineers and through SpecSearch the Microscopy can be searched for the exact specifications needed.
Brain, Cells, Microscopy, Confocal Microscopy, Glycoprotein, Human, Immunoblotting, Lead, Mutation, Association, Axons, Brains, Capillaries, Catalytic Subunits, Co-immunoprecipitation, Disease, Family, Fibroblast, Flow Cytometry, Gamma-secretase
Core B of the PPG provides important support for all projects in standardized cell staining methods for flow cytometry and confocal microscopy, coordinates in v...
Background Reflectance confocal microscopy (RCM) images skin at cellular resolution. and has shown utility for the diagnosis of nonmelanoma skin cancer in vivo.. Topical application of aluminium chloride (AlCl3) enhances contrast in RCM. images by brightening nuclei.. Objectives To investigate feasibility of RCM imaging of shave biopsy wounds using. AlCl3 as a contrast agent.. Methods AlCl3 staining was optimized, in terms of concentration vs. immersion. time, on excised tissue ex vivo. RCM imaging protocol was tested in patients. undergoing shave biopsies. The RCM images were retrospectively analysed and. compared with the corresponding histopathology.. Results For 35% AlCl3, routinely used for haemostasis in clinic, minimum immersion. time was determined to be 1 min. We identified three consistent patterns of. margins on RCM mosaic images by varying depth: epidermal margins, peripheral. dermal margins, and deep dermal margins. Tumour islands of basal cell. carcinoma were identified at ...
Background Reflectance confocal microscopy (RCM) images skin at cellular resolution. and has shown utility for the diagnosis of nonmelanoma skin cancer in vivo.. Topical application of aluminium chloride (AlCl3) enhances contrast in RCM. images by brightening nuclei.. Objectives To investigate feasibility of RCM imaging of shave biopsy wounds using. AlCl3 as a contrast agent.. Methods AlCl3 staining was optimized, in terms of concentration vs. immersion. time, on excised tissue ex vivo. RCM imaging protocol was tested in patients. undergoing shave biopsies. The RCM images were retrospectively analysed and. compared with the corresponding histopathology.. Results For 35% AlCl3, routinely used for haemostasis in clinic, minimum immersion. time was determined to be 1 min. We identified three consistent patterns of. margins on RCM mosaic images by varying depth: epidermal margins, peripheral. dermal margins, and deep dermal margins. Tumour islands of basal cell. carcinoma were identified at ...
Time-Lapse Reflection Imaging of Matrix Assembly - Collagen, type I, or pepsin-solubilized intestinal submucosal, PSIS, was polymerized in 8-well chambered coverglass (Lab-Tek, Nalge Nunc Int.). The unstained 3-dimensional matrices were viewed with an inverted confocal laser-scanning microscope (BioRad MRC1024) using a 60X, oil immersion lens. Laser light of 488 nm illuminated the hydrated samples and the reflected light was detected with a photomultiplier tube using a blue reflection filter. Samples were maintained at 30-37°C on a heated stage. Time-lapse images of fibrillogenesis were recorded at 10-15 second intervals. For standardizing the scanning depth in the matrix, the Z position was kept in the range of 20 to 50 µm from the coverglass.. All material (text, images and movies) copyright Purdue University. No material may be reproduced in any form without the express permission of Purdue University. ...
Abstract: : Purpose: To correlate ex vivo confocal microscopy to histology and ultrastructure in human corneas after LASIK surgery. Methods: Postmortem corneas (n=6) from 4 patients with a prior history of LASIK surgery (19-30 months postop) were received from U.S. eye banks. The corneas which had been stored in Optisol GS for 3-5 days, were mounted in a chamber and the endothelium perfused with BSS Plus (37o C, 15 mm Hg) for 1 hour. Confocal microscopy with the Nidek Confoscan 3 was performed in the center of each cornea. The corneas were then processed for toluidine blue stained light microscopy and transmission electron microscopy. Normal Optisol GS stored (34 hrs to 5 days) corneas were examined in a similar manner as controls. Results: Confocal microscopy was found to produce optical sections of 30 µm in thickness which were able to clearly identify the LASIK flap interface region in all corneas. Flap thicknesses measured by confocal microscopy were found to correlate closely to the flap ...
Purpose: : We recently developed methods to enable corneal epithelial penetration by bacteria to be visualized and quantified in eyeballs of mice without tissue fixation or labeling. Here, the methods were used to determine the role of the type III secretion (T3S) system for P. aeruginosa. Methods: : Two different methods were used to enable corneal epithelial susceptibility to P. aeruginosa penetration using freshly excised eyeballs from mice; 1) After rinsing, corneas were blotted with a Kimwipe™, and 100 mM EGTA for 1 h at 37°C; or 2) MyD88 knockout mouse eyes were used. Corneas were inoculated with GFP-labeled 200 µl ~109CFU/mL P. aeruginosa strain PAO1 or an isogenic T3S (ΔexsA) mutant for 6 h, 37°C. After rinsing, corneas were imaged using 2-photon microscopy (Mai Tai laser 720 nm, NADPH autofluorescence), confocal reflection microscopy (HeNe laser 633 nm) and confocal GFP labeling (Argon Laser 488nm). Epithelial thickness and depth of bacterial penetration were compared. Efficiency ...
Laser-scanning in vivo confocal microscopy (IVCM) of the cornea is becoming an increasingly popular tool to examine the living human cornea with cellular-level detail in both healthy and pathologic states. Here, we describe the use of the IVCM technique to examine the processes of tissue healing and regeneration in the living human eye after biomaterial implantation. The regenerative response can be assessed by performing longitudinal IVCM imaging of a laboratory-made, cell-free biomaterial, after direct implantation into a pathologic eye as a primary alternative to human donor tissue transplantation.. ...
Many international researchers joined the 11th Confocal Raman Imaging Symposium from September 29160to October1601, 2014 in Ulm, Germany. The well-established conference is a popular event at which the Raman community can present and discuss its latest scientific results. Talks from various fields of application and over 20 poster presentations...
Yazawa H., Yu Z.-X., Takeda K., Le Y., Gong W., Ferrans V.J., Oppenheim J.J., Li C.C.H., Wang J.M.. The 42 amino acid form of beta amyloid (Abeta42) plays a pivotal role in neurotoxicity and the activation of mononuclear phagocytes in Alzheimers disease (AD). Our recent study revealed that FPRL1, a G-protein-coupled receptor, mediates the chemotactic and activating effect of Abeta42 on mononuclear phagocytes (monocytes and microglia), suggesting that FPRL1 may be involved in the proinflammatory responses in AD. We investigated the role of FPRL1 in cellular uptake and the subsequent fibrillar formation of Abeta42 by using fluorescence confocal microscopy. We found that upon incubation with macrophages or HEK293 cells genetically engineered to express FPRL1, Abeta42 associated with FPRL1 and the Abeta42/FPRL1 complexes were rapidly internalized into the cytoplasmic compartment. The maximal internalization of Abeta42/FPRL1 complexes occurred by 30 min after incubation. Removal of free Abeta42 from ...