1G1Y: Studies on the hydrolyzing mechanism for cyclodextrins of Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structure of the mutant E354A complexed with beta-cyclodextrin, and kinetic analyses on cyclodextrins.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
A taxonomic study was carried out to clarify the taxonomy of representatives of a group of marine actinomycetes previously designated MAR 1 and considered to belong to the family Micromonosporaceae. The organisms had phenotypic properties consistent with their assignment to this taxon. The strains formed a distinct taxon in the 16S rRNA Micromonosporaceae gene tree and shared a range of phenotypic properties that distinguished them from members of all of the genera with validly published names classified in this family. The name proposed for this novel taxon is Salinispora gen. nov. The genus contains two species recognized using a range of genotypic and phenotypic criteria, including comparative 16S-23S rRNA gene spacer region and DNA-DNA relatedness data. The names proposed for these taxa are Salinispora arenicola sp. nov., the type species, and Salinispora tropica sp. nov.; the type strains of these novel species have been deposited in service culture collections as strain CNH-643T (=ATCC BAA-917T
Modules of approx. 120 residues. Granular starch-binding function has been demonstrated in the case of Thermoactinomyces vulgaris R-47 α-amylase 1 (TVAI ...
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the ...
Chemistry, Biochemistry and Genetics of Antibiotics Biosynthesis in Actinomycetes Microorganisms produce a large variety of biologically active substances representing a vast diversity of fascinating molecular architecture not available in any other systems. Our research centers on the chemistry, biochemistry and genetics of the biosynthesis of these secondary metabolites. Our method blends organic chemistry, biochemistry, and molecular biology. Our objectives are to understand the secondary metabolism by asking the following questions: which reactions are available in nature, what are the enzymatic mechanisms of these reactions, how they are linked to produce complex structures, and what are the regulatory mechanisms of these pathways. Our ultimate goal is to manipulate natures biosynthetic machinery for the discovery of new drugs and the improvement of the existing ones. Currently we are studying two types of molecules: polyketides such as thermorubin produced by Thermoactinomyces vulgaris ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinocatenispora thailandica.
Smulevitch, S.V.; Osterman, A.L.; Galperina, O.V.; Matz, M.V.; Zagnitko, O.P.; Kadyrov, R.M.; Tsaplina, I.A.; Grishin, N.V.; Chestukhina, G.G.; Stepanov, V.M. (1991). Molecular cloning and primary structure of Thermoactinomyces vulgaris carboxypeptidase T: a metalloenzyme endowed with dual substrate specificity. FEBS Lett. 291: 75-78. doi:10.1016/0014-5793(91)81107-j. PMID 1936254 ...
Similarly to their terrestrial relatives, marine microbes are a rich source of bioactive metabolites (antibiotics, antitumor drugs) and enzymes with different applications. For instance, cultivation of a marine actinomycete known as Salinispora tropica yielded a number of novel metabolites, not found before. One of these compounds, salinosporamide A, has antitumor properties and is currently being tested in humans for the treatment of cancer. Sequencing the genome of Salinispora tropica unveiled a number of genes coding for the synthesis of 17 potential metabolites; most of these compounds had not been detected in previous culturing of the microbe. Then, the researchers used the genetic information to guide a new chemical analysis of Salinispora cultures. The analysis uncovered an additional, novel compound (salinilactam), which had a structure corresponding to that deduced from the DNA sequence ...
van Tilbeurgh, H., J. Jenkins, M. Chiadmi, J. Janin, S. J. Wodak, N. T. Mrabet, and A. M. Lambeir, Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis., Biochemistry, vol. 31, issue 24, pp. 5467-71, 1992 Jun 23. ...
van Tilbeurgh, H., J. Jenkins, M. Chiadmi, J. Janin, S. J. Wodak, N. T. Mrabet, and A. M. Lambeir, Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis., Biochemistry, vol. 31, issue 24, pp. 5467-71, 1992 Jun 23. ...
Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX complex in yeast), processing of ends, and recruitment of the DNA ligase IV (LIG4)-XRCC4 complex, which brings about ligation. A recent study shows that bacteria accomplish NHEJ using just two proteins (Ku and DNA ligase), whereas eukaryotes require many factors. NHEJ repairs DSBs at all stages of the cell cycle, bringing about the ligation of two DNA DSBs without the need for sequence homology, and so is error-prone ...
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Aprepitant EP Impurity C ; Aprepitant para-Biphenyl Impurity ; 4-Defluoro-4-(p-fluorophenyl)aprepitant ; 5-[[(2R,3S)-2-[(1R)-1-[3,5-bis(Trifluoromethyl)phenyl]ethoxy]-3-(4′-fluoro biphenyl-4-yl) morpholin-4-yl]methyl]-1,2-dihydro-3H-1,2,4-triazol-3-one and ...
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TY - JOUR. T1 - A new antimicrobial antibiotic from actinoplanes capillaceus sp. K95-5561(T). AU - Fukami, A.. AU - Nakamura, Tomonori. AU - Kawaguchi, K.. AU - Rho, M. C.. AU - Matsumoto, A.. AU - Takahashi, Y.. AU - Shiomi, K.. AU - Hayashi, M.. AU - Komiyama, K.. AU - Omura, S.. PY - 2000. Y1 - 2000. UR - http://www.scopus.com/inward/record.url?scp=0033766911&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0033766911&partnerID=8YFLogxK. M3 - Article. VL - 53. SP - 1212. EP - 1214. JO - Journal of Antibiotics. JF - Journal of Antibiotics. SN - 0021-8820. IS - 10. ER - ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinoplanes deccanensis.
Quetiapine EP Impurity C ;. 2-(2-(4-(Dibenzo[b,f][1,4]thiazepin-11-yl)piperazin-1-yl)ethoxy)ethyl 2-(4-(dibenzo[b,f][1,4]thiazepin-11-yl)piperazin-1-yl)acetate ;. CAS # 1798840-31-9 ;. C40H42N6O3S2 ;. MW: 718.93. ...
Accepted name: thermitase. Reaction: Hydrolysis of proteins, including collagen. Other names: thermophilic Streptomyces serine proteinase; Thermoactinomyces vulgaris serine proteinase. Comments: A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2] Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 69772-87-8. References 1. Mizusawa, K. and Yoshida, F. Thermophilic Streptomyces alkaline proteinase. J. Biol. Chem. 247 (1972) 6978-6984. [PMID: 4711613]. 2. Borgia, P. and Campbell, L. Properties of two homologous alkaline proteases from Streptomyces ...
Bacterial genome sequences consistently contain many more biosynthetic gene clusters encoding specialized metabolites than predicted by the compounds discovered from the respective strains. One hypothesis invoked to explain the cryptic nature of these gene clusters is that standard laboratory conditions do not provide the environmental cues needed to trigger gene expression. A potential source of such cues is other members of the bacterial community, which are logical targets for competitive interactions. In this study, we examined the effects of such interactions on specialized metabolism in the marine actinomycete Salinispora tropica. The results show that antibiotic activities and the concentration of some small molecules increase in the presence of co-occurring bacterial strains relative to monocultures. Some increases in antibiotic activity could be linked to nutrient depletion by the competitor as opposed to the production of a chemical cue. Other increases were correlated with the production of
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Gregory L. Tilford writes that the species within the Solidago genus may be used more or less interchangeably. He writes the greens can be eaten a cooked potherb, with variable palatibility, and the flowers make a nice sweetened tea. Dried leaves and flowers may be used as a styptic agent, and an infusion to reduce mucus production in the bronchi during a cold or flu. The tea is diuretic and regarded by him as a kidney tonic.[4] ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
WBGene00001198 not CELE_ in pgid 348 WBGene00001198 not CELE_ in pgid 350 WBGene00001198 not CELE_ in pgid 351 WBGene00001198 not CELE_ in pgid 352 WBGene00002126 not CELE_ in pgid 1424 WBGene00009821 not CELE_ in pgid 1520 WBGene00012263 not CELE_ in pgid 2914 WBGene00043408 not CELE_ in pgid 2914 WBGene00009175 not CELE_ in pgid 2914 WBGene00016878 not CELE_ in pgid 2914 WBGene00020512 not CELE_ in pgid 2914 WBGene00019252 not CELE_ in pgid 2914 WBGene00015732 not CELE_ in pgid 3033 WBGene00003454 not CELE_ in pgid 3330 WBGene00003440 not CELE_ in pgid 3330 WBGene00003441 not CELE_ in pgid 3330 WBGene00003427 not CELE_ in pgid 3330 WBGene00003461 not CELE_ in pgid 3330 WBGene00003459 not CELE_ in pgid 3330 WBGene00003428 not CELE_ in pgid 3330 WBGene00003447 not CELE_ in pgid 3330 WBGene00003455 not CELE_ in pgid 3330 WBGene00003453 not CELE_ in pgid 3330 WBGene00003436 not CELE_ in pgid 3330 WBGene00003439 not CELE_ in pgid 3330 WBGene00023572 not CELE_ in pgid 4733 WBGene00023572 not CELE_ ...
WBGene00001198 not CELE_ in pgid 348 WBGene00001198 not CELE_ in pgid 350 WBGene00001198 not CELE_ in pgid 351 WBGene00001198 not CELE_ in pgid 352 WBGene00002126 not CELE_ in pgid 1424 WBGene00009821 not CELE_ in pgid 1520 WBGene00012263 not CELE_ in pgid 2914 WBGene00043408 not CELE_ in pgid 2914 WBGene00009175 not CELE_ in pgid 2914 WBGene00016878 not CELE_ in pgid 2914 WBGene00020512 not CELE_ in pgid 2914 WBGene00019252 not CELE_ in pgid 2914 WBGene00015732 not CELE_ in pgid 3033 WBGene00003454 not CELE_ in pgid 3330 WBGene00003440 not CELE_ in pgid 3330 WBGene00003441 not CELE_ in pgid 3330 WBGene00003427 not CELE_ in pgid 3330 WBGene00003461 not CELE_ in pgid 3330 WBGene00003459 not CELE_ in pgid 3330 WBGene00003428 not CELE_ in pgid 3330 WBGene00003447 not CELE_ in pgid 3330 WBGene00003455 not CELE_ in pgid 3330 WBGene00003453 not CELE_ in pgid 3330 WBGene00003436 not CELE_ in pgid 3330 WBGene00003439 not CELE_ in pgid 3330 WBGene00023572 not CELE_ in pgid 4733 WBGene00023572 not CELE_ ...
Dupa ce a suferit o tragedie in familie , Mack Phillips cade intr-o depresie adanca care-l face sa se indoiasca de cele mai fundamentale convingeri ale sale . El se confrunta cu o criza in credinta si primeste o scrisoare misterioasa care-l invita intr-o baraca pustie in mijlocul salbaticiei ...
Two thousand and four is the date Ive made my plans and its great some new gear to buy to test and to try the rest is all up to fate. POG It is difficult to say what is impossible, for the dream of yesterday is the hope of today and the reality of tomorrow. Robert H. Goddard * From the AT-L , Need help? http://www.backcountry.net/faq.html ...
Florile mele- infloresc si reinfloresc . Altele pier.. Cele care rezista , daruiesc cu generozitate din cele primite in dar - parfum si bucuria culorilor.. #gallery-1 { margin: auto; } #gallery-1 .gallery-item { float: left; margin-top: 10px; text-align: center; width: 33%; } #gallery-1 img { border: 2px solid #cfcfcf; } #gallery-1 .gallery-caption { margin-left: 0; } /* see gallery_shortcode() in wp-includes/media.php */ ...
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Date privind reconcilierea calculelor trimestriale a produsului intern brut cu cele anuale; Ajustarea sezoniera a produsului intern brut, principalelor activitati economice ...
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Looking for online definition of farmer's lung in the Medical Dictionary? farmer's lung explanation free. What is farmer's lung? Meaning of farmer's lung medical term. What does farmer's lung mean?
In the wake of bortezomib and its analogs, a second generation of drugs targeting the proteasome is emerging. These drugs include salinosporamide A (NPI-0052) [27, 28] (Figure 2b), a secondary metabolite derived from a novel obligate marine actinomycete (Salinispora tropicana), and its analogs [29]. NPI-0052 is a highly potent and selective 20S proteasome inhibitor with irreversible activity against all three catalytic sites. In preclinical studies, NPI-0052 has been shown to outperform bortezomib, as it was active against myeloma cells resistant to bortezomib, steroids and thalidomide, and was less toxic to normal cells. Studies using human multiple myeloma xenografts showed that NPI-0052 is well tolerated, prolongs survival and reduces tumor recurrence, thus supporting its use to treat multiple myeloma [30]. A phase I trial was recently initiated in the US, in patients with solid tumors and lymphomas. Open analogs of salinosporamide A, such as 2-pyrrolidone derivatives from microorganisms ...
Hyaluronate and type III procollagen peptide concentrations in bronchoalveolar lavage fluid as markers of disease activity in farmers lung. Bjermer, Leif; Engstrom-Laurent, Anna; Lundgren, Rune; Rosenhall, Leif; Hallgren, Roger // British Medical Journal (Clinical Research Edition);10/3/1987, Vol. 295 Issue 6602, p803 Investigates the hyaluronate and type III procollagen peptide concentrations in bronchoalveolar lavage fluid as markers of disease activity of farmers lung. Prominence of impaired diffusion capacity; Decline of lavage fluid concentrations; Increase in risk of fibrotic lung disease. ...
Farmer s lung often presents clinically as recurring acute episodes several hours after exposure to moldy hay. During these episodes the blood neutrophil count increases. Because activated neutrophils release toxic oxygen metabolites and proteinases, we hypothesized that the pulmonary reaction in farmers lung may be induced by the secretory...
Chembest Research Laboratories Limited is a contract research company focused on the discovery of new moleculars.Chembest provides a wide range of high quality research chemicals and biochemicals including novel life-science reagents, reference compounds and Natural compounds for laboratory and scientific use,with on-time delivery at competitive pricing. Besides, We offer synthesis services of organic compounds for the pharmaceutical, drug discovery and biopharma industries and supplies chemicals including regular reagents, fine chemicals, and pharmaceutical intermediates and so on. The volume of our products ranges from milligrams to kilograms scale.
Background In the literature on family caregiving, care receiving and caregiving are treated as distinct constructs generally, suggesting that informal care and support flow inside a unidirectional manner from caregiver to care recipient. recipients as caregivers. As caregiving technology enters a new era of difficulty and maturity, there is a need for conceptual and methodological methods that acknowledge, account for, and support the complex, web-like nature of family caregiving configurations. Study that contributes to, and is educated by, a broader understanding of the reality of family caregiving will yield findings that carry greater medical relevance than has been possible previously. as those who harbor a particular disease appealing, subsequently labeling the comparative who accompanies these to, or exists within, the study placing as the for their relative focus on caregiver inattention and outcomes to care and attention recipient considerations. Similarly, caregivers will be the ...
Farmers lung, also known as extrinsic allergic alveolitis or hypersensitivity pneumonitis, is an allergic inflammatory reaction induced by inhalation of Aspergillus spores, often in exposure to mold and hay. It usually occurs after inhalation during an overwhelming exposure to spores. A multitude of other antigenic stimuli can induce a similar syndrome.
A novel filamentous actinobacterial organism, designated strain MG-37T, was isolated from a Norwegian fjord sediment and examined using a polyphasic taxonomic approach. The organism was determined to...
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ErbB1 HER1 EGFR ErbB2 HER2 Neu ErbB3 HER3 ErbB4 HER4 I II III IV I II III IV I II III IV I II III IV Extracellular Cytoplasmic Transmembrane Kinase Domain N C N N C C C-terminal tail Zahnow, C.A., Expert Rev Mol Med The EGFR family of Receptor Tyrosine Kinases { EGFRHer2/neuHer3Her4 Kinase Domain Inactive
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Costurile Arbitrajului comparativ cu cele din instanța de drept comun - între mit și realitate Author: Alina Cobuz Băgnaru, Partener Fondator, Cobuz şi Asociaţii S-a subliniat în numeroase rânduri că unul din avantajele arbitrajului este și costul scăzut al acestei proceduri, în comparație cu costurile din instanța de drept comun. Această afirmație trebuie analizată…
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YKK is hiking our trail this year, shes leaving real soon from here. To my new friend: no cheese will I send, if you keep Pantene away from your gear! POG It is difficult to say what is impossible, for the dream of yesterday is the hope of today and the reality of tomorrow. Robert H. Goddard * From the AT-L , Need help? http://www.backcountry.net/faq.html ...
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