Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments. Two immiscible phases used for the droplet generation are referred to as the continuous phase (medium in which droplets are generated) and dispersed phase (the droplet phase). The size of the generated droplets is mainly controlled by the flow rates of the continuous phase and dispersed phase, interfacial tension between two phases and the geometry used for the droplet generation. Generally, three types of microfluidic geometries are utilized for the droplet generation: (i) T-Junction, (ii) Flow Focusing, and (iii) Co-Flowing. The benefits of microfluidics can be ...
Miniaturization has been a driving force in many areas of science and technology, most notably in the electronics industry. Droplet-based microfluidics, a methode to produce emulsion drops in a controlled way and at high-throughputs, enables the miniaturization and automation of biological and chemical experiments. Each emulsion drop is used as a closed reaction vessel, enabling the performance of thousands of experiments per second, at throughputs traditional technologies cannot meet. Droplet-based microfluidics is a driving technology in the advances of genomics, proteomics, single-cell analysis, high-throughput screening, and diagnostics. Crucial for using emulsion drops as reaction vessels is that they do not break and that they are tight, not allowing material to be exchanged between drops. To prevent emulsion drops from coalescing, they can be stabilized with surfactants. They adsorb at the liquid-liquid interface, lower the interfacial tension, and add steric stability. For most drop-based
Microfluidics based Lab-on-a-chip technology exhibits an unprecedented perspective in studying microbiology and biochemistry. With microfluidic technology, researchers can manipulate and probe individual cells, and can precisely control their microenvironments. Thus microfluidics enables quantitative measurements with high biological/chemical selectivity and sensitivity, as well as high temporal and spatial resolution. This IDI proposal envisages Western becoming a national leader in multidisciplinary research and education in bionanotechnology and related subjects. As a part of the Westerns overall efforts to accomplish this, the research team plans to apply microfluidic technology to answer essential microbiology questions.. The combination of microfluidics and microbiology also opens a brand new field for education, specifically, bionanotechnology. In the aspect of education, the research team aims:. ...
Lab-on-chips (Microfluidics) market research report covering industry trends, market share, market growth analysis and projection by MIcroMarketMonitor.com. Lab-on-chips (Microfluidics) market report includes,|Key question answered| What are market estimates and forecasts; which of Lab-on-chips (Microfluidics) markets are doing well and which are not? and |Audience for this report| Lab-on-chips (Microfluidics) companies.
View more ,Bioaffinity mass spectrometry screening is a novel approach using non-denaturing electrospray ionization (ESI) mass spectrometry (MS) in identifying drug leads. This screening technique can detect and preserve noncovalent protein-active drug ligand complexes under different physiological conditions. Although there are many successful screening campaigns employing this technique, the big challenge of the screening is the reduction of sample volume needed. We demonstrate in this paper that analysis of samples can be performed using droplet-based microfluidics. Droplets of samples to be screened are formed and delivered directly into the electrospray emitter of a Fourier Transform mass spectrometer. The results show that a MS instrument with a conventional ESI source can clearly detect the samples and distinguish it with the separating oil phase. The proposed technique opens the possibility of bioaffinity mass spectrometry screening of small samples with a simple microfluidic device ...
Identification of rare cells or molecules from a mixture population is important in biology such as identification of rare cancer cells or nucleic acid in early stage cancer diagnosis. Recent advances in droplet-based microfluidics and hydrogel barcoded microsphere to capture all the mRNA molecules in each cell in a single step enables scientists to identify cells based on their whole transcriptome information. However, due to the large number of sequencing reads required to cover the whole transcriptome, this limits the number of cells processed in one sequencing run. We address this problem by using a stepwise approach by first encapsulating single cell and lysis buffer together in a water-in-oil picoliter droplet, then amplifying only the target DNA/RNA molecule of interest in each droplet, pico-inject hydrogel barcoded microsphere into each droplet to tag the amplicons prior to next generation sequencing. We demonstrated the use of this technology by applying it to study how single tumor ...
Adam R. Abate and David A. Weitz Syringe-vacuum microfluidics: A portable technique to create monodisperse emulsions Entry by [[Fei Pu]], AP 225, Fall 2012 Keywords: [[microfluidics]], [[emulsions]], [[monodisperse]] ==Summary== Monodisperse drop formation is the central operation in droplet-based microfluidics but can be quite challenging due to the need for precise, steady pumping of reagents; forming monodisperse drops with controlled properties is thus a stringent demonstration of the effectiveness of a control system.A simple method for creating monodisperse emulsions with microfluidic devices is presented. Unlike conventional approaches that require bulky pumps, control computers,and expertise with device physics to operate devices, this method requires only the microfluidic device and a hand-operated syringe. The fluids needed for the emulsion are loaded into the device inlets, while the syringe is used to create a vacuum at the device outlet; this sucks the fluids through the ...
This high-quality international symposium will bring together leading experts in all fields of droplet-based microfluidics from all around the world. We fully believe that all the presentations in this symposium will stimulate the exchange of ideas and experiences amongst all the participants. There will be four sequential sessions during the course of the two days with plenty of opportunities for discussion and interaction. The topics of the whole symposium include: ...
TY - GEN. T1 - Holographic control of droplet microfluidics. AU - Cordero, Maria-Luisa. AU - Burnham, Daniel R.. AU - Baroud, Charles N.. AU - McGloin, David. PY - 2008. Y1 - 2008. N2 - Droplet microfluidics is an emerging area in miniaturisation of chemical and biological assays, or lab-on-a-chip devices. Normally consisting of droplets flowing in rigid microfluidic channels they offer many advantages over conventional microfluidic design but lack any form of active control over the droplets. We present work, using holographic beam shaping, that allows the real time reconfigurability of microfluidic channels allowing us to redirect, slow, stop, and merge droplets with diameters of approximately 200 microns. A single beam is be sufficient to perform simple tasks on the droplets but by using holographic beam shaping we can produce multiple foci or continuous patterns of light that enable a far more versatile tool.. AB - Droplet microfluidics is an emerging area in miniaturisation of chemical ...
Electrophoresis.. 12.1 Introduction.. 12.2 Experimental Section.. 12.3 Results and Discussion.. 12.4 Applications.. 12.5 Conclusions.. 13 Chemical Separations in 3D Microfluidics.. 13.1 Introduction.. 13.2 Fabrication.. 13.3 Results and Discussion on 3D Valves.. 13.4 Microfluidic Three-Dimensional Separation Columns.. 13.5 Results on Liquid Chromatography.. 13.6 Conclusions.. 14 Enabling Fundamental Research in Proteomics.. 14.1 Introduction.. 14.2 Membrane Protein Extraction.. 14.3 Conclusion.. PART IV BIOMEDICAL APPLICATIONS OF MICROFLUIDICS.. 15 Microengineering Neural Development.. 15.1 Introduction.. 15.2 Microengineering Guidance of Axons to their Targets.. 15.3 Synaptogenesis on a Microfluidic Chip.. 15.4 Conclusions.. 16 Applications of Centrifugal Microfluidics in Biology.. 16.1 Introduction.. 16.2 Why Use Centrifugal Force for Fluid Manipulation?. 16.3 How Centrifugal Microfluidic Platforms Work.. 16.4 CD Applications.. 16.5 Conclusions.. 17 Microfluidic Techniques for Point-of-Care In ...
TY - JOUR. T1 - Merging microfluidics and sonochemistry: towards greener and more efficient micro-sono-reactors. AU - Fernandez Rivas, David. AU - Cintas, P.. AU - Gardeniers, Johannes G.E.. PY - 2012. Y1 - 2012. N2 - Microfluidics enable the manipulation of chemical reactions using very small amounts of fluid, in channels with dimensions of tens to hundreds of micrometers; so-called microstructured devices, from which the iconic image of chips emerges. The immediate attraction of microfluidics lies in its greenness: use of small quantities of reagents and solvents, and hence less waste, a precise control of reaction conditions, integration of functionality for process intensification, safer and often faster protocols, reliable scale-up, and possibility of performing multiphase reactions. Among the limitations found in microfluidics the facile formation of precipitating products should be highlighted, and in this context, the search for efficient mass and energy transfers is a must. Such ...
An integrated circuit device includes a plurality of dynamic array sections, each of which includes three or more linear conductive segments formed within its gate electrode level in a parallel manner to extend lengthwise in a first direction. An adjoining pair of dynamic array sections are positioned to have co-located portions of outer peripheral boundary segments extending in the first direction. At least one of the linear conductive segments within the gate electrode level of a given dynamic array section is a non-gate linear conductive segment that does not form a gate electrode of a transistor. The non-gate linear conductive segment of either of the adjoining pair of dynamic array sections spans the co-located portion of outer peripheral boundary segment toward the other of the adjoining pair of dynamic array sections, and is contained within gate electrode level manufacturing assurance halo portions of the adjoining pair of dynamic array
Microfluidics is a field in which very small volumes of fluids are manipulated with microfabricated structures. At this very small scale, liquids exhibit a low Reynolds number, which is a parameter that describes whether the liquid moves in ordered sheets (as is the case in microfluidics) or if the flow is more chaotic. Because the fluid flow profiles in microfluidic devices can be precisely controlled, they can be engineered to carry out chemical reactions and mixing/separation of particles. Applications are manifold; some include assay development, cell sorting, in vitro modeling, and drug screening.. We are currently developing microfluidics to purify and analyze biomarkers for the early detection of pancreatic cancer. Because pancreatic cancer often does not present with symptoms until it is too advanced to treat, it is crucial to diagnose early. Unfortunately the current method of diagnosing pancreatic cancer is not trivial: an invasive biopsy of the tissue has to be examined by a ...
Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction ...
Last week some of us had the pleasure of visiting Boston for the 2012 Workshop on Capillary-based Microfluidics for Bioanalysis, hosted by Dr. Cathie Klapperichs group at Boston University. As those of you who were there or watched the live stream [1] know, there was an incredible line-up of some of the best ideas that paper microfluidics has to offer. This community is using these paper technologies to address a huge range of problems, from identification of counterfeit drugs [2] to inexpensive solar power [3]. Inexpensive and easy-to-use devices are improving our ability to detect antibiotic resistance [4] and monitor water quality [5], while on-paper electrochemical detection [6] and nucleic acid amplification [7] increase the sensitivity achievable in these paperfluidic devices. We heard about the challenges and successes involved when testing new devices in the field [8] and got to experience do-it-yourself fabrication of little devices [9] and tests for TB drug adherence [10].. I didnt ...
Microfluidics is the science and technology that deals with the flow of liquids from microliters (mL) to picoliters (pL) inside a micrometer sized channels1. The ability of microfluidics to miniaturize and mimic various laboratory procedures with limited space, great efficiency and low sample volumes these systems find use in various applications involving continuous flow microfluidics, droplet-based microfluidics, DNA chips (microarrays), molecular biology, etc.. A microfluidic chip is a set of microchannels molded into materials such as glass, silicon or polymers like polydimethyl siloxane (PDMS) 1.. Microfluidic platforms allow physicians to analyze a single drop of blood or urine sample for various markers of diseases without the need of sending comparatively large volumes of these samples to external laboratories for analysis2.. To determine a disease state, microfluidic sensors require specific disease-detecting biomolecules to be inserted into the platform of the system. These ...
With the increasing use of microfluidics, there is a need for a rather general experimental approach in order to monitor and characterize transport effects. Indeed, micro-fabrication methods have allowed the inclusion of numerous new structures and devices within microfluidics channels, and such alterations in flow patterns should impact solute transport characteristics. In the present contribution, Raman microscopy is combined with band-target entropy minimization analysis (BTEM) in order to rapidly assess and map concentration profiles in various regions of a microfluidics device. Two isotopomers, CHCl,sub,3,/sub, and CDCl,sub,3,/sub,, are contacted under laminar conditions. Special consideration is given to the point of contact between the two liquids, transport in straight sections, transport in curved sections, and wall effects. Break-through curves confirmed that stagnation of fluid at the wall is not occurring, despite substantial wall roughness. Since the methods used in the present ...
Turbulence is commonly viewed as a type of macroflow, where the Reynolds number (Re) has to be sufficiently high. In microfluidics, when Re is below or on the order of 1 and fast mixing is required, so far only chaotic flow has been reported to enhance mixing based on previous publications since turbulence is believed not to be possible to generate in such a low Re microflow. There is even a lack of velocimeter that can measure turbulence in microchannels. In this work, we report a direct observation of the existence of turbulence in microfluidics with Re on the order of 1 in a pressure driven flow under electrokinetic forcing using a novel velocimeter having ultrahigh spatiotemporal resolution. The work could provide a new method to control flow and transport phenomena in lab-on-a-chip and a new perspective on turbulence.
9781596931343: Pressure-Driven Microfluidics, Vaclav Tesar - Microfluidics in an emerging technological area involving the handling of small fluid flows in channels and devices so small that a whole system, capable of performing complex tasks, can be held
Microfluidics, which is classified as either active or passive, is capable of separating cells of interest from a complex and heterogeneous sample. Active methods utilise external fields such as electric, magnetic, acoustic, and optical to drive cells for separation, while passive methods utilise channel structures, intrinsic hydrodynamic forces, and steric hindrances to manipulate cells. However, when processing complex biological samples such as whole blood with rare cells, separation with a single module microfluidic device is difficult. Hybrid microfluidics is an emerging technique, which utilises active and passive methods whilst fulfilling higher requirements for stable performance, versatility, and convenience, including (i) the ability to process multi-target cells, (ii) enhanced ability for multiplexed separation, (iii) higher sensitivity, and (iv) tunability for a wider operational range. This review introduces the fundamental physics and typical formats for subclasses of hybrid microfluidic
Advances in Microfluidics and Nanofluidics is an international and interdisciplinary conference with special focus on research activities in the Pacific Rim. It will be held at the beautiful campus of HKUST (http://www.ust.hk/eng/index.htm) by the bay. The last decade has seen exponential growths in microfluidic and nanofluidic research in Asia, driven by robust funding with expectation that it will spur a large Asian biotechnology industry. The intent of this conference is to bring researchers of different disciplines and nationalities together, which is necessary for the Asian community to advance to the next level. It is also an opportunity to expose Asian research achievements to leaders in the field and for Asian students to interact with them. Biomicrofluidics (http://bmf.aip.org), an American Institute of Physics journal, will be the affiliated journal to fascilitate the missions of this conference, and subsequent follow-up conferences.. The invited speakers are leading microfluidics and ...
Microfluidics technology has contributed to powerful tools that have helped advance many areas of biology. The applications of microfluidic systems in chemistry and biochemistry have increased in number over the years with the development of new components and processes for the injection, mixing, pumping, and storing of fluids in the microchannels.
Generally, glass-based microfluidics with the chemical inertness and thermal stability have widely used, while plastics including PDMS have showed their convenience in fabricating microfluidic devices. Therefore, one can simply anticipate that it must be desirable to combine the reliable properties of the glass material and the economic processibility of the plastics. In this point of view, it is an interesting fact that most preceramic polymers convert into the unique inorganic and organic hybrid phase. Moreover, the polymers have been fabricated using near-net shape processing techniques. In this report we have described the fabrication and microfluidic application of preceramic polymer-derived transparent, solvent resistant, thermally stable and bio-compatible microchannels and substrates. The simple micromolding technique was used to fabricate channels by thermal and photo crosslinking of a commercially available poly(vinyl silazane) or hydridopolycarbosilane. Both thermal and photo cured polymer
Coalescence of two kinds of pre-processed droplets is necessary to perform chemical and biological assays in droplet-based microfluidics. However, a robust technique to accomplish this does not exist. Here we present a microfluidic device to synchronize the reinjection of two different kinds of droplets and
The project would include but not be limited to applications of single molecule spectroscopy to understanding of protein folding reactions. Candidates should have a recent Ph.D. in engineering, chemistry, physics, materials science or a related field. The work will involve design, fabrication & characterization of the microfluidics devices and their applications. The candidate would have access to research facilities at both at Center for Biophotonics S&T at UC Davis and at Lawrence Livermore National Laboratory. Opportunities also exist for close collaboration with Single Molecule Biophysics Group at UCLA http://smb.chem.ucla.edu/ and with the laboratory of Prof. L. Lapidus at Michigan State (http://www.pa.msu.edu/~lapidus/introduction.htm). Experience in as many of the following disciplines is not necessary but it would be a plus: computational fluid dynamics, micro- and nano-fabrication, microfluidics, fluorescence microscopy. The ideal candidate would be a person with experience in one of ...
The integration of membranes in microfluidics has attracted significant interest over the last two decades to offer precise mass transport for filtration, extraction, and gas-liquid exchange.1,2 One of the most important attributes to the mass transport of membranes for selective subjects is semi-permeability. The transport is based on a difference in chemical potential between the two sides of a semi-permeable membrane that allows selective subjects to diffuse through.3 Since the membrane porosity governs the transport, it is highly desired that the porosity can be actively tuned and customized to enhance the implementation of integrated membranes. Many approaches have been employed to integrate membranes into microfluidics, including direct incorporation of commercially available membranes, membrane preparation as part of the chip fabrication process, in situ preparation of membranes, and the direct use of the membrane properties of a bulk chip material.3 Among them, the in situ biofabrication ...
Studying microfluidics is simple with this software. Model single- and two-phase creeping flow, electrophoretic, electrokinetic, electroosmotic behavior…
Studying microfluidics is simple with this software. Model single- and two-phase creeping flow, electrophoretic, electrokinetic, electroosmotic behavior…
Health, ... New Academic Research Collaboration (ARC) program makes cutting-edg...Newton MA (PRWEB) May 17 2010 -- Microfluidics (OTCBB: MFLU) the ex...,Microfluidics,Promotes,Scientific,University,Research,Using,Nanotechnology,with,Innovation,Incentives,,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Bacterial chemotaxis, a remarkable behavioral trait which allows bacteria to sense and respond to chemical gradients in the environment, has implications in a broad range of fields including but not limited to disease pathogenesis, in-situ bioremediation and marine biogeochemistry. And therefore, studying bacterial chemotaxis is of significant importance to scientists and engineers alike. Microfluidics has revolutionized the way we study the motile behavior of cells by enabling observations at high spatial and temporal resolution in carefully controlled microenvironments. This thesis aims to explore the potential of microfluidic technology in studying bacterial behavior by investigating different aspects of bacterial chemotaxis on a microfluidic platform. We quantified population-scale transport parameters of bacteria using videomicroscopy and cell tracking in controlled chemoattractant gradients. Previously, transport parameters have been derived theoretically from single-cell swimming behavior ...
Dolomite, a world leader in microfluidic applications, is pleased to announce the launch of its Membrane Chip Interface, a novel device designed specifically for in-line liquid-liquid separation, liquid-liquid contacting, dead-end and cross-flow filtration, and electrochemistry applications.
RPA and microfluidics: A team at the University of Southampton has developed a digital microfluidic chip device that exploits RPA and on-chip fluorescence to quantitatively detect antimicrobial resistance genes in DNA extracted from patient samples.
TY - THES. T1 - Apoptosis chip for drug screening. AU - Wolbers, F.. N1 - The research described in this thesis was supported by Medisch Spectrum Twente, Hospital Group, Enschede, The Netherlands. PY - 2007/6/8. Y1 - 2007/6/8. N2 - This thesis describes the process of the development towards a microfluidic platform to perform apoptosis studies on chip in real-time at a single-cell level. The requirements for such cell-based assays are discussed as well as the advantages of using microfluidics for cellular analysis. Our main interest is focused on breast cancer cells. Ultimately, this microfluidic chip has to function as a new analytical device for the clinic to provide patients with the best individual therapy.. AB - This thesis describes the process of the development towards a microfluidic platform to perform apoptosis studies on chip in real-time at a single-cell level. The requirements for such cell-based assays are discussed as well as the advantages of using microfluidics for cellular ...
Microfluidics is still a fast growing field and an interesting market, which increasingly demands sophisticated equipment and specific engineering solutions. Aside from the widely discussed chip technology, the external equipment and machinery to operate such a microfluidic chip system comes into focus. A number of companies offer solutions to pursue the various requests made by the microfluidic community. Commercially available systems for pumping fluids are versatile but also highly expensive. Here, we present a fully-programmable pressure source, which is low-cost and can be utilized for pressure-controlled fluid driving, destructive bonding tests, and other pressure-relevant experiments. We evaluated our setup and compared the performance to a commercially available system. Furthermore, we demonstrated the use of the system in the field of droplet microfluidics as a possible application. Our development aims to lower the entrance threshold for microfluidic technology and make it more accessible to a
COC & COP microfluidic cartridge. At microLIQUID we have 10 years of experience working side by side with our customers, manufacturing microfluidic products based on hard polymers such as COC (cyclic olephine copolymer) and COP (cyclic olephine polymer). Our state-of-the-art micro-manufacturing processes for these types of microfluidic cartridges are unique in the industry.. These medical-grade plastics are perfect for microfluidics, as they are outstanding performers in different life science applications: molecular diagnostics, immunoassays, NGS, cell culture and cell diagnostics. These highly biocompatible copolymers have exceptional optical properties, including high clarity and high light transmissivity, in addition to very low moisture absorption, very low permeability to water vapor, and good chemical resistance.. We apply different high-skill engineering processes to our in-house manufacture of COC and COP microfluidic devices, undertaking the complete workflow from initial concept to ...
Technology advances have driven a genomics revolution with sweeping impact on our understanding of life processes. Nevertheless, the arguably more important proteomics revolution remains unrealized. Proteins are complex; meaning that multiple physicochemical properties must be assayed. Consequently, proteomic studies are resource intensive and data limited. To drive a bold transformation of biomedicine, engineering innovation in proteomics instrumentation is needed. While microfluidic technology has advanced separations science, progress lags in the multi-stage separations that are a hallmark of proteomics. This talk will summarize new microengineering design strategies for critical multi-stage protein assays. Specifically, I will introduce our tunable photopatterned materials for switchable function, microfluidic architectures for seamless integration of discrete stages, and multiplexed readouts for quantitation. In a translational example, I will detail assay and design advances from our ...
MreB, an actin-like cytoskeleton in E. coli encoded by MreB gene, is essential for the maintenance of bacterial cell shape. Our project is aimed at the cell-free synthesis of MreB, and the actin homolog MreB was further characterized through both microfluidics technology and nanobiotechnology. As in vivo, MreB is rigidly linked to the cell wall. Here, MreB was cell-free expressed in vitro. Protein localization on the inner oil film could be observed in the W/O/W microdroplets produced with microfluidic technologies, which mimics the cell membrane to provide a non-polar environment. Furthermore, CNTs (carbon nanotubes) were involved as scaffolds for the auxiliary expression of actin homolog MreB. The combination of MreB with CNTs and protein aggregation were observed though the AFM (atomic force microscope) in vitro. Our research cell-free synthesizes the actin-like cytoskeleton filaments, and provides a sound microenvironment for protein synthesis, which helps to recognize, explore and create ...
Single-cell transcriptomic profiling is rapidly advancing in throughput while its cost declines thanks to innovative single cell manipulation technologies, clever barcoding, and next generation sequencing. Microfluidics recently expanded throughput by enabling isolation, lysis, and RNA capture in continuous trains of aqueous nanoliter droplets, making it possible to profile ,10,000 single cells in one experiment. Unfortunately, a focus on fresh cells left behind the vast collection of potentially informative frozen tissue samples. Nuclei from archived samples are teeming with RNA transcripts that have been successfully sequenced with low throughput techniques. Now, Habib and colleagues leverage the scalability of droplet-based microfluidics to perform high-throughput single-nucleus RNA-Seq on frozen archived mouse and human samples in a method they call DroNc-Seq.. They began by optimizing microfluidic channel dimensions to maximize the number of transcripts detected and minimize the occurrence ...
Book Description Amazon Link , CRC Press Link Author: Liang-Yin Chu, Wei Wang ISBN: 978-3-527-34182-5 336 pages June 2017 Summary An increasing number of technologies are [more...] ...
Browse Full Report @ https://www.reportsandmarkets.com/reports/1208363-global-microfluidics-technologies-market-growth-trends-forecasts-2017-2022 The Globa
Dr. Holger Becker is co-founder and CSO of microfluidic ChipShop GmbH. He obtained physics degrees from the University of Western Australia/Perth and the University of Heidelberg. He started to work on miniaturized systems for chemical analysis during his PhD-thesis at Heidelberg university, where he obtained his PhD in 1995. Between 1995 and 1997 he was a Research Associate at Imperial College with Prof. Andreas Manz. In 1998 he joined Jenoptik Mikrotechnik GmbH. Since then, he founded and led several companies in the field of microsystem technologies in medicine and the life sciences. He lead the Industry Group of the German Physical Society between 2004 and 2009 and is Conference Chair for the SPIE Microfluidics, BioMEMS and Medical Microsystems conference as well as acting as regular reviewer of project proposals on national and EU level and for several journals devoted to microsystem technologies. ...
Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. Microfluidic droplets, which can be generated, manipulated and interrogated at very high throughput, are isolated reaction vessels at the single cell scale. Compartmentalization maintains the genotype-phenotype link, making droplet microfluidics suitable for screening of extracellular traits such as secreted products and for screening of microcolonies originating from single cells.. In Paper I, we investigated the impact of droplet microfluidic incubation formats on cell culture conditions and found that syringe and semi open incubation resulted in different metabolic profiles. Controlling culture conditions is key to cell factory screening, as product formation is influenced by the state of the ...
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The behavior of fluids on the microscale may differ from macrofluids in factors such as surface tension, energy dissipation, and fluidic resistance at the beginning to dominate the system. Microfluidics studies these behavioral changes and how they can be avoided or exploited for new uses.. On a small scale (diameters between 100 nanometers and several hundred micrometers, approximately) some properties of special interest appear. The Reynolds number, which characterizes the presence of turbulent flow, is extremely low, so laminar flow will be maintained. In this way, joining two fluids could not easily mix through turbulence, so the diffusion must be done by itself in the two fluids to be mixed.. ...
MicruX is focused on the design, development and manufacture of novel miniaturized analytical systems based on Lab-on-a-Chip, microfluidics and electrochemistry.
TY - JOUR. T1 - Multiplex analysis of enzyme kinetics and inhibition by droplet microfluidics using picoinjectors. AU - Sjostrom, Staffan L.. AU - Jönsson, Håkan. AU - Svahn, Helene Andersson. PY - 2013. Y1 - 2013. N2 - Enzyme kinetics and inhibition is important for a wide range of disciplines including pharmacology, medicine and industrial bioprocess technology. We present a novel microdroplet-based device for extensive characterization of the reaction kinetics of enzyme substrate inhibitor systems in a single experiment utilizing an integrated droplet picoinjector for bioanalysis. This device enables the scanning of multiple fluorescently-barcoded inhibitor concentrations and substrate conditions in a single, highly time-resolved experiment yielding the Michaelis constant (Km), the turnover number (kcat) and the enzyme inhibitor dissociation constants (ki, ki′). Using this device we determine Km and kcat for β-galactosidase and the fluorogenic substrate Resorufin β-d-galactopyranoside ...
MIKROFLUIDIK UND NANOFLUIDIK; MIKROPARTIKEL + NANOPARTIKEL + MIKROSPHÄREN + NANOSPHÄREN (NANOTECHNOLOGIE); SELBSTORGANISATION (NANOSTRUKTURIERTE MATERIALIEN); MICROFLUIDICS AND NANOFLUIDICS; MICROPARTICLES + MICROSPHERES + NANOPARTICLES + NANOSPHERES (NANOTECHNOLOGY); SELF-ORGANIZATION (NANOSTRUCTURED MATERIALS ...
NEWTON, MA , Posted on January 10th, 2008. Utilizing Microfluidics fixed geometry diamond interaction chamber technology and ceramic plunger, the M-110P easily enables the production of nano-suspensions and nano-emulsions, as well as liposomal encapsulation and cell disruption with the fewest number of passes. Additionally, the M-110P allows flow rates between 110-155 ml/minute depending on process pressure, which is a higher flow rate than any comparable, competitive product. This flow rate enables the efficient processing of pharmaceutical formulations, fine chemicals, and biological material ranging from simple oil-in-water emulsions to highly immiscible solids-in-liquid suspensions. All results from the M-110P machine are guaranteed to be scaleable to pilot and/or production volumes.. ####. About Microfluidics ...
Laboratory of microfluidic technologies for biomedicine: MicroRNA, miRNA, organ-on-chip, microfluidics, toxin, viscumin, ricin, laminin, placenta, intestine, gut, liver, brain, blood-brain barrier, cancer, tumor
University of Tennessee at Knoxville Doctoral student, Peter Golden Shankles, presents his dissertation on Interfacing to Biological Systems Using Microfluidics, discussing the popular new field of microfluidics and the 3D printed tools that are propelling it forward-for this project and numerous others recently too. 3D printing allows for much greater self-sustainability in the lab as researchers can create tools for experiments and chemical reactions on their own, but attention must also be paid to how such technologies affect chemical transformations and influence biological systems.. As microfluidics are used more frequently with cell-free protein synthesis systems (CFPS), researchers usually set up tubes to experiment with reactions. As the author points out, this is usually easy, but other studies have shown better success with engineered reaction hardware. In this study, the researchers aim to begin using microfluidics as well as nanoscale membranes to lessen distances in diffusion ...
Microfluidic devices have a wide variety of biological applications. My Ph.D. dissertation focuses on three major projects. A) culturing a non-adherent immortal cell line within a microfluidic device under static and dynamic media flow conditions; B) designing and fabricating novel microfluidic devices for electrokinetic injecting analytes from a hydrodynamic fluid; and C) using this novel injection method to lyse single non-adherent cells by applying a high electric field across the cell at a microfluidic channel intersection. There are several potential advantages to the use of microfluidic devices for the analysis of single cells: First, cells can be handled with care and precision while being transported in the microfluidic channels. Second, cell culturing, handling, and analysis can be integrated together in a single, compact microfluidic device. Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. In this ...
The use of microfluidic systems for screening of aptamers and their biomedical applications are reviewed in this paper. Aptamers with different nucleic acid sequences have been extensively studied and the results demonstrated a strong binding affinity to target molecules such that they can be used as promising candidate biomarkers for diagnosis and therapeutics. Recently, the aptamer screening protocol has been conducted with microfluidic-based devices. Furthermore, aptamer affinity screening by a microfluidic-based method has demonstrated remarkable advantages over competing traditional methods. In this paper, we first reviewed microfluidic systems which demonstrated efficient and rapid screening of a specific aptamer. Then, the clinical applications of screened aptamers, also performed by microfluidic systems, are further reviewed. These automated microfluidic systems can provide advantages over their conventional counterparts including more compactness, faster analysis, less sample/reagent
Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of
misc{8894052, abstract = {Microfluidic applications nowadays have become of great interest due to their broad compatibility especially in biological applications, and one of them being droplet-based cell encapsulation. Cell encapsulation in droplets is carried out by discretising an aqueous phase (i.e. cell suspension) and including them into a continuous oil phase. This methodology is a potential gateway to high throughput droplet-based cell fusion (e.g. for the production of hybridomas). The challenge here is to achieve a high efficiency of correctly paired cells in a droplet to overcome the random fusion pairing during bulk cell fusion. As such, droplet microfluidics can be used to co-encapsulate a single cell A and a single cell B cells in one droplet or encapsulate cells separately and merge droplets with desired cell number and type subsequently with other droplet manipulations. In this study, separate encapsulation studies of human B lymphocytes and mouse embryonic stem cells were ...
The Microfluidics Lab aims to design and fabricate microfluidic devices for clinical applications and to study important cellular issues. With much progress in microfluidics in the last decade, microfluidics has become an enabling technology for miniaturization, or lab-on-a-chip platform. In our Lab, cell-based studies are the focus of research.. The Labs cell culture room (CCR) cultures about 8 cell lines, supplying fresh cells for experiments. The Labs cleanroom provides essential facilities for fabricate of microdevices. Additional microfabrication needs are supported by various centers on campus.. The most important asset of the Lab is people. Current, 2 doctoral students working closely with 4 master students in a team-work atmosphere to mutually support each others research needs. Collaboration with surgeons and doctors in the National Taiwan University Hospital as well as other faculty members complements our engineering know-how to enable important research in the cellular ...
Optical coherence tomography (OCT) angiography (OCTA) has been actively studied as a noninvasive imaging technology to generate retinal blood vessel network maps for the diagnoses of retinal diseases. Given that the uses of OCT and OCTA have increased in the field of ophthalmology, it is necessary to develop retinal phantoms for clinical OCT for product development, performance evaluation, calibration, certification, medical device licensing, and production processes. We developed a retinal layer-mimicking phantom with microfluidic channels based on microfluidic fabrication technology using polydimethylsiloxane (PDMS) and titanium dioxide (TiO2) powder. We implemented superficial and deep retinal vessels using microfluidic channels. In addition, multilayered thin films were synthesized with multiple spin-coating processes that comprised layers that corresponded to the retinal layers, including the ganglion cell layer (GCL), inner plexiform layer (IPL), and inner nuclear layer (INL). The phantom ...
The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. A general introduction of applications of optical tweezers and microfluidics is given in chapter 1. Theoretical concepts related to optical trapping and microfluidics are reviewed in chapter 2, followed by a detailed description of the instrumentation in chapter 3. In chapter 4, ligand-receptor interactions are studied under physiological conditions: whole cells or native vesicles carrying in their membrane the protein of interest are immobilized first in the laser trap inside a microfluidic channel, then the reaction is initiated by changing the solution in the region around the trap. In chapter 5 and chapter 6 respectively, surface-modified polystyrene beads are used to study ligand-receptor interactions and DNA hybridization. The examples of ...
Electrophoretic separation in nanofluidic channels exhibits significant differences with microfluidics. We discuss a theoretical / experimental collaboration investigating particle separation by electropohoresis in nanochannels. Recent experimental results in the laboratory of our collaborator Dr. Pennathur (UCSB, Dept. ME) indicate that increased fidelity can be achieved in separating particles by size and charge when using channels with cross sections of nanometer dimensions (100nm x 1000nm), as opposed to larger microfluidic channels. For short double-strands of DNA (10 - 100 base pairs) it is found that separation in microfluidic channels produces electropherograms with only one lumped peak. However, for nanofluidic channels several clearly distinct peaks are observed. Given the small dimensions of the nanofluidic channel, it is expected that new effects which were relatively weak in microfluidic channels play an important role. Identifying how these underlying mechanisms augment electrophoretic
The purpose of this thesis is to study the crystallization in a microfluidic device of an active pharmaceutical ingredient which is (2S)-2-[(4R)-2-oxo- 4-propylpyrrolidin-1-yl] butanamide, with product name Brivaracetam. This molecule is manufactured by the Belgian pharmaceutical industry UCB, and a better understanding of its crystallization in the microscopic scale, and especially its polymorphism, would lead to new possibilities in order to develop a future industrial continuous crystallizer based in the microfluidic technology. For this purpose, several experiments have been run, both in the macroscopic scale and using the microfluidics technology. The solubility curve for the system was determined, and also a cluster formation was analysed using volumes around 10ml of solution. Several crystallizations were done also with solutions of this volume to first understand the polymorphism that the solute presents. For the micro-scale crystallizations, the microfluidic device used was a system ...
A microfluidic platform for the rapid determination of distribution coefficients by gravity assisted droplet-based liquid-liquid extraction, C. E. Poulsen, R. C. R. Wootton, A. Wolff, A. J. deMello & K. S. Elvira, Analytical Chemistry, 2015, 87, 6265. The aim of this research was to use microfluidic technologies to provide an original improvement to a method that has not been updated in decades, namely the determination of distribution coefficients. The platform provides clear advantages to the traditional shake-flask method (it is 48 times faster, uses 99% less reagents, has very low user sensitivity and higher reproducibility), whilst being as simple as possible: the platform makes use of picolitre-sized droplets to take advantage of the large surface-area-to-volume ratios, but uses a separation chamber that falls in the milli-fluidic regime to enable the use of gravity for phase separation. To allow this platform to be used in commercial settings, it was fabricated using cyclic olefin ...
The widespread dissemination of CTX-M extended spectrum β-lactamases among Escherichia coli bacteria, both in nosocomial and community environments, is a challenge for diagnostic bacteriology laboratories. We describe a rapid and sensitive detection system for analysis of DNA containing the blaCTX-M …
A variety of pulmonary diseases such as COPD, asthma, ARDS are profoundly associated with the surfactant dysfunction that leads to liquid plug formation across the airway lumen [19]. Several animal model studies have shown during such lung disorders severe tissue-level damage to the distal lung airways due to repeated closure and reopening process [4]. To mimic exactly the in vivo conditions, Huh et al. [20] developed a compartmentalized microfluidic airway models and demonstrated that the reopening of occluded microfluidic airway causes severe injury of pulmonary epithelial cells [20]. In the lung airways, rupturing of the liquid plugs leads to abnormal breath sounds known as crackles. To simulate this scenario, a three-dimensional (3D) microfluidic device was developed to detect acoustically the crackling sound and it was demonstrated that there is a higher risk of cell injury when liquid plugs become very thin. They demonstrated cellular level of lung injury under flow condition using this ...
TY - JOUR. T1 - A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay. AU - Huang, Cheng Yeh. AU - Tsai, Po Yen. AU - Lee, I. Chin. AU - Hsu, Hsin-Yun. AU - Huang, Hong Yuan. AU - Fan, Shih Kang. AU - Yao, Da Jeng. AU - Liu, Cheng Hsien. AU - Hsu, Wen-Syang. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Here, we describe a technique to manipulate a low number of beads to achieve high washing efficiency with zero bead loss in the washing process of a digital microfluidic (DMF) immunoassay. Previously, two magnetic bead extraction methods were reported in the DMF platform: (1) single-side electrowetting method and (2) double-side electrowetting method. The first approach could provide high washing efficiency, but it required a large number of beads. The second approach could reduce the required number of beads, but it was inefficient where multiple washes were required. More importantly, bead loss during the washing process was unavoidable in both methods. ...
The market study on Global Microfluidic Devices Market 2017 Research Report studies current as well as future aspects of the Microfluidic Devices Market primarily based upon factors on which the companies compete in the market, key trends and segmentation analysis. This report covers each side of the worldwide market, ranging from the fundamental market info and advancing more to varied important criteria, based on that, the Microfluidic Devices market is segmented. Microfluidic Devices industry research report analyzes, tracks, and presents the global market size of the major players in every region around the world. Furthermore, the report provides data of the leading market players in the Microfluidic Devices market.. This report studies Microfluidic Devices in Global market, especially in North America, China, Europe, Southeast Asia, Japan and India, with production, revenue, consumption, import and export in these regions, from 2012 to 2017, and forecast to 2022.. Request for FREE Sample ...
By taking advantage of the natural movement of liquid through paper, researchers at Harvards Whitesides Research Group may have found a way to make microfluidics technology much cheaper. The result could be disposable diagnostic tests simple and abundant enough for use in the developing world.. The field of microfluidics deals with the precise manipulation of tiny quantities of liquid. One of its most promising applications is the so-called lab-on-a-chip, which can work with much smaller fluid samples than larger devices require, potentially allowing for more portable diagnostic tools. But existing microfluidic chips are generally made from comparatively expensive materials like silicon, glass, or plastic and have tiny pumps and valves that can be difficult to manufacture.. Now, Harvards George Whitesides and his team have built a microfluidic device on a square of paper the size of a pinky fingernail. Its the first example Ive heard of paper microfluidics, says Albert Folch, a bioengineer ...
Microfluidic devices are analogous to circuit boards, and they can be programmed to perform all kinds of laboratory tasks on a small scale. They have the potential to perform all kinds of medical tests involving body fluids in a short time and using very small samples.. While circuit boards pass electricity, which can be abstracted and quantified as bits, microfluidic devices tend to work with liquids that can mix with one another and contaminate each other. For microfluidic devices to approach the logic abilities of circuit boards, the fluids within have to somehow be perfectly separated from each other until the time that theyre expected to mix. Conventional microfluidic gates and valves arent adequate in this context, so researchers at Duke University have now developed a way to keep individual droplets from touching each other while moving them around using sound waves inside a microfluidic device.. Scaling up this approach could lead to programmable and rewritable microfluidics that can ...
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
Health, ...ANN ARBOR Mich. Cancer cells are on the move in the bloodstream in t...In a study of 51 patients researchers used a state-of-the art microfl...The findings published in Gastroenterology suggest that circu... While there is much work that still needs to be done there is great ...,Microfluidic,technology,reveals,potential,biomarker,for,early,pancreatic,cancer,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Engineering cellular microenvironments that more accurately reflect the in vivo situation is now recognized as being crucial for the improvement of the in vitro viability and in vivo-like function of cells or tissues. Microfluidic technologies have been increasingly applied since the late 1990s for this purpose, with a growing number of examples of perfused cell and tissue cultures in microfluidic chambers and channels. The well-defined solution flows provided by microfluidics mean enhanced cell growth and function through improved nutrient delivery and waste removal. Additional benefits include the implementation of well-defined temporal and spatial (bio)chemical gradients, and mechanical signals that cells experience in their natural environment. Because the ability to culture cells and tissue under such controlled conditions leads to cellular function that is distinctly more organ-like, the microfluidic systems used are now referred to as organs-on-a-chip or microphysiological systems. ...
Microlytic was founded in 2006 with the intention to use microfluidic technology to help solve the major problems in structural biology. Microlytic sought to develop and produce microfluidic chips that offered a high probability of crystallizing target proteins with a set-up that is simple and easy to use.. Microlytic developed microfluidic chips to let users grow crystals large enough to be used immediately for X-ray diffraction, plus give users direct access to the crystals themselves. These goals were realized with the development of the Crystal Former - the first microfluidic platform to allow users to go from crystallization screen to structure using a single device.. The advantages of the Crystal Former are ...
In order to overcome these challenges, we aim to develop a novel breast cancer model that incorporates the relevant properties of the three-dimensional microenvironment. For this purpose, we use microfluidic technology, which enables us to manipulate and control fluids at the small scale. Cell encapsulation is used to first generate soft, cell-containing beads that mimic the basement membrane. These beads are then embedded in a more fibrous matrix that mimics the stromal ECM, completing the tissue model.
Video articles in JoVE about microfabrication include Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies, Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation, Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening, Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps, Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks, A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)-Cell Interaction and the Resultant Bioeffects at the Single-cell Level, Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles, Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light, A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological
Microfluidic technology may offer faster and more controlled ways to produce radiotracers for medical imaging, including studies into Parkinsons disease, thanks to a first of its kind approach in the Southern Hemisphere ...
If you wanted to know if your child had a fever or be certain that the roast in the oven was thoroughly cooked, you would, of course, use a thermometer that you trusted to give accurate readings at any temperature within its range. However, it isnt that simple for researchers who need to measure temperatures in microfluidic systemstiny, channel-lined devices used in medical diagnostics, DNA forensics and lab-on-a-chip chemical analyzersas their current thermometer can only be precisely calibrated for one reference temperature. Now, researchers at the National Institute of Standards and Technology (NIST) have proposed a mathematical solution that enables researchers to calibrate the thermometer for microfluidic systems so that all temperatures are covered.. Reactions taking place in microfluidic systems often require heating, meaning that users must accurately monitor temperature changes in fluid volumes ranging from a few microliters (a droplet approximately 1 millimeter in diameter) ...
The 18-well titer plate works with the spacing of a 96-well microtiter plate namely 9 mm and is available in different materials and in transparent and colored versions It can be used with our adapter frame in microtiter-plate format that is made as a special adapter for microfluidic chips in
Further details of the study can be found in the paper titled 3D-printed bioreactors for DNA amplification: application to companion diagnostics. It is co-authored by A.K. Pantazis, G. Papadakis, K. Parasyris, A. Stavrinidis, and E. Gizeli.. The DNA amplification process comes under microfluidics, a field of study that 3D printing has benefited greatly in recent years (and vice versa). Earlier in 2020, a team of researchers from UC Davis published a paper detailing the development of a new droplet-based 3D printing method using microfluidics. The technique allows the user to manipulate the extruded ink composition and properties in real time, enabling the fabrication of diverse structures. The team expects the technology to excel in soft robotics and tissue engineering.. Elsewhere, in Europe, researchers produced and published a review on 3D printing microfluidic applications. In the review the scientists state that the technology will allow for the creation of a new generation of increasingly ...
Microfluidics is the study of precise control and manipulation of fluids that are geometrically constrained to a small, normally sub millimetre, range. It has application in various fields like engineering, physics, chemistry, biochemistry, nanotechnology and biotechnology, from real applications to the plan of systems in which little volumes of fluids are used to attain multiplexing, automation etc. It has appeared in the beginning of the 1980s and is used in the expansion of inkjet print heads, DNA chips, lab-on-a-chip technology, micro-propulsion and micro-thermal technologies.. ...
Microfluidic Environments and Cell BehaviorThe use of microfluidics for the study of basic biology is still in its infancy with the focus to date on the use of microfluidics for acute analysis. Another possible use of microfluidics and more broadly micro systems, is for the longer term growth, study and even production of living systems. In this presentation, I will describe work towards this end involving the development of a fabrication platform that makes possible integrated organic and biomimetic microsystems. Further, preliminary evidence suggesting that these systems can, in some cases, provide a more nature (or in vivo-like) micro environment for living systems will be described. The effect of microenvironments on the behavior of a variety of living systems (insect cells, yeast, mammalian embryos, human embryonic stem cells and mammary epithelial cells) will be summarized.. April 1, ...
Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine.
Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine.
Project Description: Grade IV astrocytomas known as glioblastoma multiforme (GBM) are the most aggressive primary brain tumors, with a median survival rate of 15 months after diagnosis. Current methodologies for diagnosis include time-consuming histopathological reviews of biopsied brain tumor tissue to determine malignancy, which can delay early diagnosis and treatment. We propose to use microfluidics in combination with labeled magnetic beads to separate invasive glioma cells from other healthy neural tissue within a few hours. A strategy that combines rapid cell sorting with subsequent cell culture of a pure population of the patients glioma cells could not only speed up time to diagnosis, but also enhance personalized treatment. We hypothesize that incubating magnetic beads labeled with anti-epithelial cell adhesion molecule (epCAM) antibody will allow for the selective separation of glioma cells from other neural tissue cell types from co-culture, as judged by recent evidence that glioma ...
Lin BC. 1996-2012:From Capillary Electrophoresis to Intelligent Control Digital Microfluidics[C]. 见:12th Asia-Pacific International Symposium on Capillary Electrophoresis and Microscale Separation and Analysis. 新加坡. 2012-12-16 ...
Gurinder has a PhD in Chemical Engineering from Loughborugh University. She has a background in Microbiology which she combined with encapsulation techniques to bring the two disciplines together. She has been a post-doc prior to joining Blacktrace during which she worked with phages to combat farm to fork food safety. She has previously been a customer of Dolomite which is the reason why she joined the team March 2019. At Dolomite, she works as a material scientist carrying out application work, performing customer feasibility studies and supporting the sales and marketing team. She is currently involved in a project addressing high-throughput liposome production.. ...