An attempt has been made to develop the genetics of Micrococcus radiodurans by analysing transformation and conjugation processes. The nature of the transformation process in M. radiodurans was studied by investigating the effect of various factors on the efficiency of transformations. M. radiodurans can be transformed by exogenous DNA at a frequency of 10 without special treatment. The presence of glucose or amino acids during growth or transformation had no significant effect on the frequency. Treatment of recipient cells with calcium chloride at the optimum concentration of 30 mM increased the frequency of transformation up to 10,000-fold for some markers, e.g. rifampicin resistance. Magnesium and strontium ions could not replace calcium while sine ions completely inhibited transformation. The efficiency of transformation was also affected by the pH, pH 7.0 being the most effective with CaCl-treated cells. The minimum time required for phenotypic expression varied wiith different markers, ...
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article{7fb96577-46ba-4de6-8791-54c72ea71182, abstract = {Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results.}, ...
I know its growth temperature is around 26 degrees celsius but it is a really resistant bacteria. I was wondering if anyone knew if it could survive in higher (30-40 degrease) or lower (0-5) temperatures ...
Micrococcus bacterium. Coloured transmission electron micrograph (TEM) of a section through a Micrococcus sp. bacterium. The bacteriums cell wall is yellow and the cell contents are blue- green. This Gram-positive aerobic bacterium is found in raw milk and on mammalian skin. It is not generally pathogenic in humans. It is capable of dividing in more than one plane, and often forms clusters of bacteria. Magnification: x73,000 at 6x7cm size. Magnification: x250,000 at 7.5x9.5 size. - Stock Image B220/1119
TY - JOUR. T1 - Micrococci and peptidoglycan activate TLR2 →Myd88 →IRAK→TRAF→NIK→IKK→NF-κB signal transduction pathway that induces transcription of interleukin-8. AU - Wang, Q.. AU - Dziarski, R.. AU - Kirschning, C. J.. AU - Muzio, M.. AU - Gupta, D.. PY - 2001/4/5. Y1 - 2001/4/5. N2 - This study was done to elucidate the signal transduction pathway of interleukin-8 (IL-8) induction by gram-positive bacteria. Bacteria (micrococci) and peptidoglycan (PGN) induced transcription of IL-8 in HEK293 cells expressing Toll-like receptor 2 (TLR2) and CD14 but not in those expressing TLR1 or TLR4. A mutation within the NF-κB site in the IL-8 promoter abrogated transcriptional induction of IL-8 by the two stimulants. Dominant negative myeloid differentiation protein (MyD88), IL-1 receptor-associated kinase (IRAK), NFκB-inducing kinase (NIK), and IκB kinase (IKK) mutant forms completely inhibited micrococcus- and PGN-induced activation of NF-κB and expression of the gene for IL-8. ...
A preparation of ATPase from the membranes of Micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. They did not correspond to isoenzymes because one band could be converted into the other by exposure to a mildly alkaline pH value. The conversion was paralleled by changes in molecular weight, circular dichroism and catalytic properties. Denaturation by pH at 25 degrees C was followed by means of circular dichroism, ultracentrifugation and polyacrylamide gel electrophoresis. A large conformational transition took place in the acid range with midpoints at about pH = 3.6 (I = 10(-4) M), 4.3 (I = 0.03 M) and 5.3 (I = 0.1 M). The transition was irreversible. Strong aggregation of the protein occurred in this range of pH. The final product was largely random coil, but even at pH 1.5 dissociation into individual subunits was not complete. However, partial dissociation took place at pH 5 (I = 0.028 M). At this pH value the enzyme
M. P. Basilyev. Hydrobionts Soluble Collagen. The stages of production of a biotechnological preparation "Micobaс"for soil purification from PAH are presented. The microorganisms effective РАН degrading are searched and isolated from samples of the soil polluted with benz(а)pyrene and benzofluoranthene. Their transformation ability in relation to phenanthrene and benz(а)pyrene is investigated in laboratory and nature. The allocated cultures are identified by a method 16S r RNA as Micrococcus sp. and Bacillus sphaericus. The biopreparation has passed testing on toxicity and pathogenicity. Key words: bacillus, poliaromatic hydrocarbon, bioremediation, bacteriummicrococcus. M. P. Vasilyev. Hydrobionts Soluble Collagen. Comparative estimation of hydrobiont and animal collagens ismade. Basing on publications data the features of fish treatment products collagen, as applied to its soluble preparation educing possible processes, are defined. Fish initial raw materials such as scales, fins studies ...
Scanning Electron Micrograph (SEM) the Gram-positive bacteria species Micrococcus agilis. Micrococcus occurs in a wide range of environments, including water, dust, and soil. Magnification unknown. - Stock Image C028/2210
Agrococcus terreus sp. nov. and Micrococcus terreus sp. nov., isolated from forest soil.: Two bacterial strains, DNG5T and V3M1T, isolated from forest soil of t
Other names: ATCC 29093, CCM 2140, CCUG 33028, CIP 81.71, D. nishinomiyaensis, DSM 20448, Dermacoccus nishinomiyaensis, Dermacoccus nishinomiyensis, Dermatococcus nishinomiyaensis, Dermatococcus nishinomiyensis, IEGM 393, IFO 15356, JCM 11613, LMG 14222, Micrococcus nishinomiyaensis, Micrococcus nishinomyaensis, NBRC 15356, NCTC 11039 ...
Micrococcus luteus is a bacterium that belongs to the family Micrococcaceae. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal flora of the mammalian skin. The bacterium also colonizes the human mouth, mucosae, oropharynx and upper respiratory tract. It was discovered by Sir Alexander Fleming before he discovered Penicillin in 1928.. M. luteus is considered a contaminant in sick patients and is resistant by slowing of major metabolic processes and induction of unique genes.. This artwork was commissioned in 2017 for BathASU.. ...
The culturability of several actinobacteria is controlled by resuscitation-promoting factors (Rpfs). These are proteins containing a c. 70-residue domain that adopts a lysozyme-like fold. The invariant catalytic glutamate residue found in lysozyme and various bacterial lytic transglycosylases is also conserved in the Rpf proteins. Rpf from Micrococcus luteus, the founder member of this protein family, is indeed a muralytic enzyme, as revealed by its activity in zymograms containing M. luteus cell walls and its ability to (i) cause lysis of Escherichia coli when expressed and secreted into the periplasm; (ii) release fluorescent material from fluorescamine-labelled cell walls of M. luteus; and (iii) hydrolyse the artificial lysozyme substrate, 4-methylumbelliferyl-β-d-N,N,N-triacetylchitotrioside. Rpf activity was reduced but not completely abolished when the invariant glutamate residue was altered. Moreover, none of the other acidic residues in the Rpf domain was absolutely required for ...
Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, Coccus, saprotrophic bacterium that belongs to the family Micrococcaceae.
Dormancy among nonsporulating actinobacteria is now a widely accepted phenomenon. In Micrococcus luteus, the resuscitation of dormant cells is caused by a small secreted protein (resuscitation-promoting factor, or Rpf) that is found in "spent culture medium." Rpf is encoded by a single essential gene in M. luteus. Homologs of Rpf are widespread among the high G + C Gram-positive bacteria, including mycobacteria and streptomycetes, and most organisms make several functionally redundant proteins. M. luteus Rpf comprises a lysozyme-like domain that is necessary and sufficient for activity connected through a short linker region to a LysM motif, which is present in a number of cell-wall-associated enzymes. Muralytic activity is responsible for resuscitation. In this report, we characterized a number of environmental isolates of M. luteus, including several recovered from amber. There was substantial variation in the predicted rpf gene product. While the lysozyme-like and LysM domains showed little ...
Micrococcus luteus ATCC ® 10240™ Designation: 130.21 TypeStrain=False Application: Assay of bacitracin Quality control strain Food testing Pharmaceutical and Personal Care
Discovered in 1956 by A. W. Anderson when trying to completely sterilize food through radiation, Deinococcus radiodurans (ATCC number 13939) is one of the extremophilic bacterium of the world. Though it was first called Micrococcus radiodurans, the genus Micrococcus did not quite match all of the characteristics that this microbe exhibited, therefore it was placed in its own genus Deinococcus which most closely matches the genus Thermus. The Greek phrase deino kokkus means "terrible berry" while the Latin words radius and durare means "radiation surviving" producing the name Deinococcus radiodurans.. A. W. Anderson and his coworkers were working on a way to sterilize food by using high doses of gamma radiation. They exposed a tin can of meat to a dose of radiation that was thought to be strong enough to kill all known forms of life. However, the meat still spoiled. Puzzled that anything could survive such a dose, they investigated only to find that the microbe D. radiodurans was the cause. This ...
Definition of Family micrococcaceae with photos and pictures, translations, sample usage, and additional links for more information.
The isolation and identification of food pathogens from freshly milled palm oil as well as the effect of steam sterilization on some quality parameters of palm oil was evaluated. Microbial isolations and quality parameters were carried out at day 0, day 14 and day 28. Biochemical parameters such as Peroxide value, Anisidine value, Free fatty acid, Deterioration of bleachibility index (DOBI) and Carotene value was analyzed in the same samples using the digital Palmoiltester. The most frequently isolated bacteria from the unsterilized samples were Pseudomonas aerugenosa, Bacillus subtilis, Enterococcus aerogenes, Staphylococcus saprophyticus and Micrococcus varians while the most frequently isolated fungal species were Aspergillus niger aggregate (IMI number 503810), Cochliobolus sp. (anamorphic state: Curvularia) (IMI number 503811), Penicillium citrinum (IMI number 503812) and a yeast, Meyerozyma guilliermondii (IMI number 503813). The fungal count in the
1]Zhang, L., Sun, D., Bo, Y. (2011). Physiological and biochemical characteristics and desilication ability of Bacillus mucilaginosus screened from Poyang Lake area of Jiandxi province. Advanced Materials Research. 308-310(1):1604-1608. Retrieved from: https://doi.org/10.4028/www.scientific.net/AMR.308-310.1604 [2]Yang, X., Li, Y., Lu, A., Wang, H., Zhu, Y., Ding, H., Wang, X. (2016). Effect of Bacillus mucilaginosus D4B1 on the structure and soil-conservation-related properties of montmorillonite. Applied Clay Science. 119(1):141-145. Retrieved from: https://doi.org/10.1016/j.clay.2015.08.033. [3]Lian, B., Chen, Y., Zhao, J., Teng, HH., Zhu, L., Yuan, S. (2008). Microbial flocculation by Bacillus mucilaginosus: Applications and mechanisms. Bioresource Technology. 99(11):4825-31. Retrieved from: https://www.ncbi.nlm.nih.gov/pubmed/17967531. [4]Friedman, B.A. and Dugan, P.R. (1968). Identification of Zooglea species and the Relationship to Zoogleal Matrix and Floc Formation. Journal of ...
Hydrocarbon utilizing bacteria were isolated from diesel impacted sites at the University of Port Harcourt using the vapour phase transfer method. The isolates were identified as Bacillus, Pseudomonas, Acinetobacter, Serratia, and Micrococcus species. Examination of cultures supplemented with glucose revealed that the total viable counts ranged from 1.7 x 104 to 5.9 x 105 cfu/ml. For cultures supplemented with kerosene the total viable counts ranged from 1.18 x 104 to 3.1 x 105 cfu/ml. Bacillus spp. and Micrococcus spp. gave the highest and lowest counts respectively in both media types within 96 h. The other isolates had counts in between these ranges in the respective supplemented media. The growth of the isolates in mineral salts solution supplemented with glucose or kerosene resulted in turbidity of the broth as compared to clear media solutions in controls. Higher turbidity was recorded in media supplemented with glucose than kerosene, and this was reflected in the total viable counts ...
Using the selective fractionation technique, a group of researchers at Brazils National Synchrotron Radiation Laboratory (LNLS) in Campinas were able to select silver nanoparticles of different sizes obtained from the same batch chemical reaction. They then evaluated the effect of these nanoparticles on different bacterial strains: Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis and Micrococcus lysodeikticus. They found that smaller nanoparticles inhibited the growth of these bacteria more effectively than larger ones. The results of this work, "Size-selective silver nanoparticles: future of biomedical devices with enhanced bactericidal properties," are featured on the cover of issue No. 33 of the Journal of Materials Chemistry.. Several scientific challenges must still be solved before silver nanoparticles can be converted into drugs. "One of these challenges is in their toxicity, since it is necessary to ascertain how these nanoparticles interact with living cells," explains ...
Micrococcus yunnanensis ATCC ® 7468™ Designation: TypeStrain=False Application: Assay of bacitracin Assay of restriction endonuclease Mlu I Food testing
Bacillus Mucilaginosus is also called silicate bacterium, whose important characteristic can break down the silicate and aluminum silicate and the potassium, silica in the minerals such as feldspar, mica and also can break down the phosphorous in apatite.. Bacillus mucilaginosus has the function of dissolving phosphorus, releasing potassium and silicon, fixing nitrogen. At the same time, it can also secrete natural plant hormones and a lot of enzymes, which can increase crop resistance to some diseases.. At present, this kind of bacteria is the best strain as biological fertilizer to promote the conversion of invalid phosphorus and potassium in soil and increase crop yields. ...
토양으로 부터 분리, 동정된 Micrococcus sp. No-32는 pH 10.3에서 잘 증식하고 ethylene을 생성하는 균주이다. 이 균주로 부터 ethylene 생산성을 높이기 위해 intact cell에 의한 발효조건을 조사하였다. 회분발효시 최적 ethylene 생성온도는 30℃, 초기 pH는 10.2, 초기 접종농도는 0.4 mg of DCW/ml of culture였다. 각종 아미노산, 유기산, 당류의 다양한 기질전환효과를 검토한 결과 대체로 아미노산과 유기산의 효과가 높았다. 특히 methionine에서 효과가 있었으며 적정농도는 균의 증식과 ethylene생성에도 좋은 100 mM로 결정하였다. 빛과 산소에 의한 영향을 살펴보면 빛과 산소가 있을 때가 없을시보다 약 2배에 가까운 ethylene을 생성하였고, 진탕배양이 정치배양보다 5배 높은 ethylene 생성량을 나타냈다. 반연속식 발효시 배양액의 50, 40, 30, 20%를 새 배지로 치환해 주었는데 치환율이 낮을 ...
Gram-positive sphere. Found in milk and milk products. Incubate at 30°C. In nutrient broth. NOTE: Bacterial and fungal cultures will be sold and shipped to institutions only. …
Hello,Did somebody use EnzChek Lysozyme Assay Kit(invitrogen,molecular probes)? I meet some difficulty in determining lysozyme activity using the EnzChek Lysozyme Assay Kit(invitrogen,molecular probes). the fluorescence of samples is very low and out of standard curve. I dont know why. principles of kit are as follows ...
In this study, we examined the bacterial communities on the upper arms of 8 volunteers before and after inoculation with H. ducreyi. The microbiomes were similar to those of reported forearm microbiomes (1, 2, 5). At preinfection, we found the skin of the deltoid region to be largely dominated by Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Similarly, a recent study on two human volunteers who were asked not to bathe 3 days prior to sampling identified these 4 phyla and Cyanobacteria on the upper arm (38). In contrast, the same study found low levels of Propionibacterium and Staphylococcus on the upper arm, whereas these genera comprised a large proportion of our volunteers microbiomes. Both studies identified moderate levels of Corynebacterium on the upper arm, and we found moderate levels of Streptococcus, Paracoccus, and Micrococcus species. We did not ask our participants to refrain from bathing prior to infection; perhaps hygiene and person-to-person variations ...
Recent work by Dimtroglou et al (2007) demonstrated that Bio-Mos significantly reduced the bacterial load in the gut of both rainbow trout and sea bream by reducing the total aerobically cultivated bacteria. In the case of sea bream, the percentage contribution of the various aerobically cultivated bacterial families to the total count was not significantly altered between the Bio-Mos fed fish and the controls. However, in the case of rainbow trout, the Bio-Mos fed fish had reduced numbers of Micrococcus spp., Staphylococcus spp., Aeromonas/ Vibrio spp and other unidentified Gram + bacteria. Increases were also seen in Acinetobacteria spp., Pseudomonads spp. and Enterobacteria spp ...
Young, M, Artsatbanov, V, Beller, HR, Chandra, G, Chater, KF, Dover, LG, Goh, E-B, Kahan, T, Kaprelyants, AS, Kyrpides, N, Lapidus, A, Lowry, SR, Lykidis, A, Mahillon, J, Markowitz, V, Mavromatis, K, Mukamolova, GV, Oren, A, Rokem, JS, Smith, MCM, Young, DI & Greenblatt, CL 2010, Genome sequence of the Fleming strain of Micrococcus luteus, a simple free-living actinobacterium Journal of Bacteriology, vol 192, no. 3, pp. 841-860. DOI: 10.1128/JB.01254-09 ...
Here you can find the definitions list for the word Gaffkya anaerobia. Also you can find some other opposite words using the online search on our website.
Principal Investigator:EZURA Yoshio, Project Period (FY):1992 - 1994, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:Fisheries chemistry
Restasis - If the conditions do not favor this encircling of the kidney by the omentum and form i tion of adhesions, then the animal promptly succumbs to fatal discovery of a special micrococcus in the blood in cases of scurvy, which reproduces the principal manifestations (hemorrhages) of the disease in animals.
extracellular space, lysozyme activity, cell wall macromolecule catabolic process, defense response to Gram-negative bacterium, defense response to Gram-positive bacterium
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Micrococcus luteus produced 29 {mu}M riboflavin during growth on 6.5 mM pyridine but not during growth on other substrates. On the basic of the results of radiolabelling studies, riboflavin was not directly synthesized from pyridine. Pyridine may interfere with riboflavin biosynthesis or elicit a general stress response in M. luteus. The optimum concentration of pyridine for both growth of the organism and pyridine degradation was 13 mM. Above 25 mM, pyridine temporarily inhibited growth, pyridine degradation, oxygen uptake, and pigment production. ...
Historically, the genera Staphylococcus and Micrococcus were placed together with the genera Stomatococcusand Planococcus in the family Micrococcaceae containing grampositive, catalase-positive cocci. An unrelated species of gram-positive cocci exhibiting positive catalase reaction and occurring in human specimens is Alloiococcus otitis, the only species of this genus, which is a member of the Carnobacteriaceae family belonging to the order Lactobacillales. In this chapter, the term
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
TY - JOUR. T1 - Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects. AU - Uddin, Khan. AU - Orelma, Hannes. AU - Mohammadi, Pezhman. AU - Borghei, Maryam. AU - Laine, Janne. AU - Linder, Markus. AU - Rojas, Orlando. PY - 2017/7/1. Y1 - 2017/7/1. N2 - Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a ...
Smelly feet can not only be an embarrassment but can seriously damage the self-esteem. There are many people, especially guys, who have to deal with this problem every day, and also are the victims of numerous medicines that didnt help. To help them I wrote this article hopping to at least make them understand what is causing this problem and how to prevent it. So here it is.. Smelly feet, also known as "bromhidrosis", are the result of the interaction between perspiration and the bacteria that lurk in your shoes and socks. The foot and hands contain the most sweat glands than any other part of the body (about 3000 glands per square inch). Inside the shoe, the temperature reaches sometimes 102 F. So this temperature combined with the moisture generated by the sweat glands constitute the perfect environment and a fertile breeding ground for the bacteria responsible for your smelly feet.. The bacteria that are often found in foot odour are usually corynebacterium and Micrococcus species. They ...
The micrococcal flora of the skin of 382 children and 378 adults have been investigated using a scheme derived from the work of Baird-Parker (1963). The predominant groups are Staphylococcus type 2 and Micrococcus type 2. Some differences in the carriage at different sites have been found: in general the nose, axillae, groin, and toe webs have a similar flora but this differs a little from other, glabrous skin sites.. ...
A total of 50 samples were examined, which consisted of 25 coins and currency notes each. Among the currency screened, 10 coins and 15 notes showed growth of single bacterial species, 11 coins and 9 notes showed the growth of two types of bacterial species each, and three coins and one note had grown three different organisms. The common fungal isolate from two of the coins was Aspergillus niger (n = 2). The bacterial isolates include Staphylococcus aureus (n = 11), Klebsiella spp. (n = 6), Escherichia coli (n = 01), Staphylococcus epidermidis (n = 3), Citrobacter spp. (n = 7), Pseudomonas spp. (n = 4), Acinetobacter spp. (n = 4), Enterobacter agglomerans (n = 2), Micrococcus spp. (n = 1), α - haemolytic Streptococcus spp. (n = 1) and Bacillus spp. (n = 35).. We have observed that 96% of the coins and 100% of the currency notes were found to be contaminated with different bacterial species, of which S. aureus, Klebsiella spp. and E. coli can be considered as pathogens, as they have been ...
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The cellular mechanisms of the force-frequency relations are then discussed. Bcl-2 expression prevents both apoptosis and the induction of these kinases. Purification and characterization of an endonuclease from Micrococcus luteus that acts on depurinated and carcinogen-modified DNA. However, a few months viagra without prescription after initial presentation, he developed signs and symptoms suggestive of hydrocephalus. In contrast, Tax protein targets a central region of IKK-gamma, which consists of amino acids 201-250.. The most important one is the myelin growth which can be observed when some poorly water soluble surfactants such as phosphatidylcholine (PC), Aerosol-OT (AOT), etc. These trends have been partially attributed to improvement in therapeutic strategies. The assessment of serum cardiac troponin I concentrations in dogs with a range of nonprimary cardiac illnesses has revealed that cardiac myocyte damage is commonplace in many generic cialis tadalafil canine diseases. Recreational ...
Beller, Harry R., Ee-Been Goh, and Jay D. Keasling. "Genes Involved in Long-Chain Alkene Biosynthesis in Micrococcus luteus ." Applied and Environmental Microbiology 76.4 (2010): 1212-1223. Web. 28 Jan. 2020. ...
Thanks to Scepheo for helping out with the console sorted list. Updated to: page 85 Total number of requested games: 1385 Platform sort: [spoiler=Arcade] Dungeons and Dragons: Shadow over Mystara Dungeons and Dragons: Tower of Doom Castlevania: The Arcade Teenage Mutant Ninja Turtles The Simpsons Three Wonders [Midnight Wanderers: Quest for the Chariot] X-Men [/spoiler][spoiler=Atari 2600] Adventure Dark Chambers H.E.R.O. Haunted House Jungle Hunt Montezumas Revenge Pitfall! Raiders of the Lost Ark River Raid Secret Quest Shuttle Orbiter Solaris Space Shuttle Superman Xenophobe [/spoiler][spoiler=Apple II] Karateka Mabels Mansion [/spoiler][spoiler=Amiga] Alien Breed 3D Alien Breed 3D II Genetic Species Giana Sisters Lionheart New Zeeland Story Rodland [2-player co-op] [/spoiler][spoiler=C64] Chips Challenge Great Giana Sisters The Neverending Story [/spoiler][spoiler=3DO] Captain Quazar D Road Rash Space Ace [/spoiler][spoiler=TG16] Bloody Wolf Dragon Saber JJ & Jeff Neutopia ...
4) If you receive an advance copy of the novel in some official capacity (e.g. reviewer, translator, etc.), the posting of MAJOR spoilers (major character deaths, major secret plot points, etc.) from that source prior to the release date is forbidden. Small spoilers and tantalizing details will be allowed (e.g. SOS, give us a spoiler PLEASE! What, like the fact that the Imperial Remnant has been hiding an SSD this whole time?), but the staff will be using their judgment to try and preserve as much of the surprise as possible. If you receive your copy via purchasing from a legitimate source (e.g. Amazon, your local bookstore, Half.com, etc.) in prior to the release date, you are free to post spoilers, provided they follow the other rules listed in these guidelines ...
According to new research, odor-causing bacteria called micrococci grow much stronger in polyester t-shirts rather than cotton, making them smellier.
Be blushingly beautiful with the Spoiler Blush Stick! Its sebum-absorbing properties leave a fresh and powdery finish! Available in 3 shades on BeautyMNL!
Warning: Spoilers ahead! The Walking Dead will return on October 14...but it doesnt look like one character will be fighting for survival for long. A series regular has been confirmed to fall victim to the zombies.
Ok, folks! Since some people have sprinted to the end in less than ten hours after it came out, this is the Unascended Spoiler/Discussion thread! What did you think? What did you like/dislike? What are you wondering about? What are you speculating about? What did you think of the backstory? Of the…
The revelations from the Alpha of Legion continue in this third installment of the Spoilers series. There have been multiple new builds in the Legion Al...
WWE taped November 15ths WWE SmackDown episode from the Phones 4u Arena in Manchester, England. Here are full WWE SmackDown spoilers.
ONESI: Upon the Fallen Update 1: Sunrise HE 3196 - HE 3500 Maps: [spoiler] Cities: [spoiler] Climates: [spoiler] [/spoiler] Of all the regions of...
WARNING! THERE MAY BE SPOILERS IN THIS THREAD SO KEEP CLEAR UNTIL YOUVE FINISHED THE GAME. I thought that since people have already gotten hold of the full game, wed kick off our reviews thread. This thread is for your reviews, not any websites or magazines (we have a thread for that here in General Discussion). Give us your personal reviews of your experience of BioShock, and a score if you want. Please be sensible and treat the thread well, keep to posting coherent and collected
A reasonably common usage of spoiler tags is to make image-heavy posts/threads more accessible. However in the current XF implementation, images within...
The Spoiler Title trope as used in popular culture. Work and episode titles that give away what happens in the episode. This trope only applies if the title …
WILL MOST DEFINITELY CONTAIN SPOILERS. This thread is to, of course, discuss the One Piece manga. If you have anything to say about the manga series, please discuss it here. If whatever you want to say is in the Japanese manga only, and the scene or character has not appeared in America yet, please use spoiler tags. Yeah. Speaking of the Japanese One Piece, I just found out some information about Chapter 426, the one with the color spread. Even though this is in spoilers, I think youre
WILL MOST DEFINITELY CONTAIN SPOILERS. This thread is to, of course, discuss the One Piece manga. If you have anything to say about the manga series, please discuss it here. If whatever you want to say is in the Japanese manga only, and the scene or character has not appeared in America yet, please use spoiler tags. Yeah. Speaking of the Japanese One Piece, I just found out some information about Chapter 426, the one with the color spread. Even though this is in spoilers, I think youre
The dude playing Aquaman looked familiar, but I cant place him? Anybody know who this guy is? As Triplet pointed out to me earlier, he looks like a...
Considering Charizard was the first thing taken down in the last battle against Brandon (I believe it was a Dusclops) it probably wouldnt have helped much...
I thought jump start a thread to discuss the next episode: Hidden. Im just Spoiling(bad pun) to talk a little about it. For starters, it seems...
...  ELISA gives a fast, and reliable method to detect Bacitracin in tissue from Randox Food Diagnostics 
Originally Posted by girishv Hi, can you please post a side profile of your car. Would like to see how the spoiler looks from the side. girish These are what I have. I dont have exact side profile
Post Edited by Administrator to reflect Major Spoilers Alert What did everyone think about Mementos ending? Was Leonard really revenge-crazy and...
Citation: Wei Pridgeon, Y., Klesius, P.H. 2014. Chicken-type lysozyme in channel catfish: Expression analysis, lysozyme activity and efficacy as immunostimulant against Aeromonas hydrophila infection. Proceedings of Aquaculture America 2014. p. 416. Interpretive Summary: Technical Abstract: To understand whether chicken-type lysozyme (Lys-c) in channel catfish was induced by infection of Aeromonas hydrophila, the transcriptional levels of Lys-c in skin, gut, liver, spleen, posterior kidney, and blood cells in healthy channel catfish was compared to that in channel catfish infected with A. hydrophila by bath immersion. Quantitative PCR revealed that the transcription levels of Lys-c in infected catfish were significantly (P , 0.05) induced in all five tissues tested as well as in blood cells. Recombinant CC-Lys-c produced in Escherichia coli expression system (R-CC-Lys-c) exhibited significant (P , 0.05) lytic activity to Gram-positive Micrococcus lysodeikticus and Gram-negative A. hydrophila. ...
The soil sample was collected from Agriculture University Gwalior, Madhya Pradesh, India which is having a history of repeated pesticide application. Bacterium capable of degrading Malathion and Dichlorvos were isolated and identify as Staphylococcus sp. Micrococcus sp. Entrobacte sp. Bordetella sp. Pseudomonas sp. and Klebsella sp. The growth of all six pesticide degrading isolates was assessed in Mineral Salt Medium (MSM) canting 100 mg/lit of each pesticide. The maximum growth rate by the isolates Pseudomonas sp. AUG12 were 1.564 and 1.435 for Malathion and Dichlorvos respectively after 140 h Plate assay revealed that Pseudomonas sp. AUG12 could grow with high concentration of Malathion (1900 mg/lit) and Dichlorvos (1500 mg/lit). The total protein concentration was higher in the supernatant of Pseudomonas sp. AUG12 extracellular fraction which is 97 µg/mL for Malathion and 99 µg/mL for Dichlorvos. The beast Pseudomonas sp. AUG12 was therefore used in further experiment. The maximum growth ret of
Armand-Lefevre L, Ruimy R, Andremont A. 2005. Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs. Emerg Infect Dis 11:711-714.. Aubry-Damon H, Grenet K, Sall-Ndiaye P, Che D, Cordeiro E, Bougnoux ME, et al. 2004. Antimicrobial resistance in commensal flora of pig farmers. Emerg Infect Dis 10:873-879.. Becker K, von Eiff C. 2011. Staphylococcus, Micrococcus, and other catalase-positive cocci. In: Manual of Clinical Microbiology (Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW, eds). 10th ed. Washington, DC:ASM Press, 308-330.. Bisdorff B, Scholhölter JL, Claußen K, Pulz M, Nowak D, Radon K. 2012. MRSA-ST398 in livestock farmers and neighbouring residents in a rural area in Germany. Epidemiol Infect 140:1800-1808; doi: 10.1017/S0950268811002378.. Burkholder J, Libra B, Weyer P, Heathcote S, Kolpin D, Thorne PS, et al. 2007. Impacts of waste from concentrated animal feeding operations on water quality. Environ Health ...
Putrescine oxidase from Rhodococcus erythropolis (PuO) is a flavin-containing amine oxidase from the monoamine oxidase family that performs oxidative deamination of aliphatic diamines. In this study we report pre-steady-state kinetic analyses of the enzyme with the use of single- and double-mixing stopped-flow spectroscopy and putrescine as a substrate. During the fast and irreversible reductive half-reaction no radical intermediates were observed, suggesting a direct hydride transfer from the substrate to the FAD. The rate constant of flavin reoxidation depends on the ligand binding; when the imine product was bound to the enzyme the rate constant was higher than with free enzyme species. Similar results were obtained with product-mimicking ligands and this indicates that a ternary complex is formed during catalysis. The obtained kinetic data were used together with steady-state rate equations derived for ping-pong, ordered sequential and bifurcated mechanisms to explore which mechanism is operative.
Study of the Micrococcaceae and Staphylococcaceae throughout the Manufacture of Dry-Cured Lacón (a Spanish Traditional Meat Product) Made without or with Additives
Sushi and sashimi are traditional Japanese food, mostly consisting of raw seafood alone or in combination with rice. Eating sushi and sashimi has become popular in many countries even outside Japan. This food is not free from health risks such as ingestion of pathogenic bacteria or parasite. The aim of this study was to investigate on hygienic-sanitary quality of sushi and sashimi sold in the cities of Messina and Catania, Southern Italy. Fifty samples (38 sushi and 12 sashimi) were analysed to determinate the aerobic mesophilic bacteria (AMB), psycrophilic bacteria (PB), Enterobacteriaceae, specific spoilage organisms (SSOs), Pseudomonas spp., coagulase-positive staphylococci, micrococci, Vibrio spp., Bacillus cereus, Salmonella spp. and Listeria monocytogenes. In sushi, AMB ranged from 5.00 to 8.18 log CFU/g, PB from 4.70 to 7.13 log CFU/g, Enterobacteriaceae from 1.41 to 6.67 log CFU/g, while SSOs and Pseudomonas spp. from 3.49 to 7.72 and from 3.36 to 8.00 log CFU/g, respectively. Micrococci ...
bacteria resuscitation-promoting factor: 16-kDa protein isolated from Micrococcus luteus and Mycobacterium tuberculosis; GenBank Z96935 (M. luteus)
Nitrogen isotopic hetero- and homogeneity of D-{\alpha}-alanine and L-{\alpha}-alanine were investigated in microbial processes in the domain Bacteria and in chemical processes in symmetric organic synthesis. D-alanine is an enantiomer that is physiologically essential for microbial growth and metabolic maintenance. The nitrogen isotopic difference {\Delta}15ND-L (defined as {\delta}15ND-Ala - {\delta}15NL-Ala) in peptidoglycan amino acids in bacteria such as the representative gram-positive phyla Firmicutes and Actinobacteria (Enterococcus faecalis, Staphylococcus aureus, Staphylococcus staphylolyticus, Lactobacillus acidophilus, Bacillus subtilis, Micrococcus luteus, and Streptomyces sp.) tended to be 15N-depleted in D-alanine ({\Delta}15ND-L , -2.0 permil). These results suggest that the composition of isotopically heterogeneous components in these bacteria is primarily controlled by enzymatic pathways prior to formation of the bacterial cell wall. In contrast, the {\Delta}15ND-L of racemic ...
The researchers watched to see how the herbivorous insect, the cabbage semilooper Trichoplusia ni (Lepidoptera), detected and responded to a diet laced with nonpathogenic, non-infectious bacteria. In most studies to date, lab reared insects have been injected with bacterial strains, whereas in nature the insects main exposure would be from eating plants.. The larvae were reared on diets with or without an added helping of Escherichia coli and Micrococcus luteus bacteria. In the bacteria-fed larvae, general antibacterial activity was enhanced, although the activity of one key enzyme related to immune response - phenoloxidase - was inhibited. Among the eight proteins highly expressed in the hemolymph of the bacteria-fed larvae were the immune-response-related proteins arylphorin, apolipophorin III and gloverin. Significantly, the pupation time and pupal mass of bacteria-fed larvae was negatively affected by their unhealthy diet.. ...
The Toll pathway regulates innate immunity in insects and vertebrates. The Drosophila Toll receptor is activated by a processed form of a ligand, Spätzle. Spätzle-processing enzyme (SPE) is the only enzyme identified to date that functions in converting Spätzle to an active form during the immune response. In the present study, Toll activation induced by immune challenge was almost suppressed in spätzle mutant larvae and adults, whereas it was present in SPE mutant larvae challenged with Micrococcus luteus and adults challenged with Bacillus subtilis. Our data suggest that an unidentified protease besides SPE processes Spätzle under conditions of microbial challenge ...
As this chapter is about the resuscitation of uncultured or nonculturable microbes, and such microbes that are successfully resuscitated must by definition have been dormant or latent, it concentrates on the issue, particularly with reference to the actinobacteria that are the source of most of the bioactive secondary metabolites of industrial or applied interest. It shows the loss of culturability of Micrococcus luteus as a decrease in the plate count at more or less constant total count. It also shows what at first sight appears to be the resuscitation of most of these previously unculturable cells (which could not form a colony in a plate count assay) as an increase in culturable (plate) count. For different cultures, the presence of appropriate concentrations of supernatant increased the culturable count of starved cells in an most-probable-number (MPN) assay by 3 to 5 orders of magnitude: the supernatant contained a resuscitation-promoting factor (Rpf) produced by culturable cells. Pheromones are
The broodstock were injected with the vaccine at a dose of 108 CFU per fish at second gonadal maturity until spawning. Challenge tests were carried out on the offspring at 5, 10, 15 and 20 days after hatching using five different strains, both individually and in combination. The researchers, who were mainly based at Nanjing Agricultural University, observed immunological parameters in broodstock, eggs and larvae and relative per cent survival (RPS) of larvae after being challenged with pathogenic S. agalactiae. The results showed that the leukocytes, phagocytic activity, respiratory burst, lysozyme activity and antibody levels of vaccinated broodstock were at a higher level compared with unvaccinated broodstock. The high level of the lysozyme activity, antibody levels and recombination activating gene 1 (RAG1) were also observed in eggs and larvae from vaccinated broodstock. Larvae produced from vaccinated broodstock when challenged with pathogenic S. agalactiaehad RPS values over 50 percent ...
Research is intended to assess the ecological factor(s) of greatest importance in determining the population size of an aphid (Aphis varians) feeding on fireweed (Epilobium angustifolium). We manipulated the host plant (by shading, watering, and fertilizing), the size of fireweed patches, the density of a leaf-feeding beetle (Altica tombacina) which also utilizes fireweed, and the presence of predators of the aphid (primarily ladybird beetles and syrphid flies) by means of cages.. View the full abstract (39A). ...
The present study was carried out, using standard techniques, to identify and count the bacteria associated with hand washing and drying, using the same naturalistic conditions in toilets as before. Average bacterial counts were again reduced by using towels (by about 38%), the most significant decrease (58%) being in bacteria grown on nutrient agar after using paper towels. Hot air dryers again produced significant increases in all bacteria (by more than 260%). Types typical of skin (including staphylococci and micrococci) increased by an average of 169%. Skin and gut bacterial counts rose by 438%, the largest increases being in enterobacteria and coliforms, including Escherichia coli (395%). Some types and strains of the bacteria found can be pathogenic. The increases in gut bacteria are indicative of faecal contamination of the hands ...
Thanks to the Japanese scientist and co-founder of the Ajinomoto food corporation, that yummy umami flavor has been packaged into its essence, monosodium glutamate (MSG). I bought a bottle today: it looks similar to a salt shaker and costs about $5. Fairly tasteless on its own, shake it on food and you will sense a rainbow of flavor. Your tongue literally goes crazy when it feels the glutamate. As MSG crystals come in contact with water, they decompose into a glutamic acid whose taste intensity is inversely related to the acidity of the rest of the food.. Originally manufactured by fermenting wheat protein, these days food scientists rely on Micrococcus glutamicus, a bacterium that cranks out glutamic acid by the bucketful, awaiting the simple separation and purification process that turns it into MSG.. Im going to start using it on everything.. ...
streptolydigin: antibiotic isolated from culture filtrates of Streptomyces lydicus; active against gram-postive bacteria except micrococci; minor descriptor (75-87); on line & INDEX MEDICUS search ANTIBIOTICS (75-87)
The two weeks from February 12 through February 23 reveal a Port Charles disaster and one mans determination to unlock the mystery of his past. General Hospital spoilers reveal that the next ...
EDIT: Cassandra Project V1 is now available. For full story and links go to the Cassandra News page here: http://www.deusex-machina.com/cassandra-project/comms.asp Feel free to give feedback in this thread, but preferably no spoilers. Ill create a Spoilers thread for more free discussion. Like I imagined all good developers should, Im now playing Spandeau Ballets ridiculous eighties Pop hit Gold at maximum volume. Download. Hopefully youll enjoy it.
Well, Steven Moffat and the crew have provided us with an ever so convenient and frustrating mid-series break. So its that time again: Doctor Who discussion thread time! Spoilers through episode seven of series six, A Good Man Goes to War. So, which was the best episode? I dont think The Curse of the Black…
Futurama: The Late Philip J. Fry Goof (SPOILERS, big uns) - Page 7 at the largest Futurama forum PEEL. Includes Futurama episode reviews, polls, chat and more.
bleh.. that clip doesnt give anything away!!.. we all know that there is going to be a porche scene whats everyone freaking out about?? lol...! unless ur one of the MOVIE only fans... well.. u just plain shouldnt be on this site hahaha theres spoilers everywhere!!! and also... it doesnt matter if u see something on this site that reveals a scene... it usually either doesnt make the final cut and also the scene is shot plently of times and its not the same everytime so the one u see here wonte be the final. ReplyDelete ...
girl meets texas spoilers instagram icon meanings [IMG] Look here the profiles of girls and choose your girlfriend |||||Register and chat with...
The Bold and the Beautiful (B&B) spoilers reveal that the feud between Wyatt (Darin Brooks) and Bill (Don Diamont) is heating up. Now that Bill knows about
Re: Star Trek: Destiny: Gods of Night - SPOILER Thread The end doesnt justify the means. Ree should have suggested that diagnostic thing and the...
M333 Proton Exora Top Fiber Spoiler With LED,Price: RM140.00,End time 8/9/2022 12:00 AM MYT,Category: Body Kits / Exterior Accessories / Automotive
Welcome to the Malazan Re-read of the Fallen! Every post will start off with a summary of events, followed by reaction and commentary by your hosts Bill and Amanda (with Amanda, new to the series, going first), and finally comments from Tor.com readers. In this article, well cover Chapter Thirteen of House of Chains by Steven Erikson (HoC).. A fair warning before we get started: Well be discussing both novel and whole-series themes, narrative arcs that run across the entire series, and foreshadowing. Note: The summary of events will be free of major spoilers and were going to try keeping the reader comments the same. A spoiler thread has been set up for outright Malazan spoiler discussion.. CHAPTER THIRTEEN. SCENE 1. Leomans 2000-strong force is ready to leave. Dom watches as Leoman speaks to Shaik, thinking hed be just as happy if Leoman didnt return and gloating over Shaiks clear ignorance of his plans that were "settling into place to achieve her own demise." Shaik reminds Leoman he ...
Weekend chart from Box Office Mojo Tomatometer from Rotten Tomatoes How was your weekend, ONTD? spoiler code: |lj-spoiler text=spoilers!|type spoiler here|/lj-spoiler|
Pertemuan 16 Pendugaan Parameter. Matakuliah : I0134 - Metoda Statistika Tahun : 2005 Versi : Revisi. Learning Outcomes. Pada akhir pertemuan ini, diharapkan mahasiswa akan mampu : Mahasiswa dapat menghitung penduga selang dari rataan, proporsi dan varians. Outline Materi. Slideshow...
Yahoo Movies exclusive first look at two Lego playsets based on Marves upcoming Thor: Ragnarok movie - including a character we didnt know was coming
I am kind of happy that spoiler[Suzuka is back], and I think that I actually rather her as a potential partner than him with Natsume. I kind of got some enjoyment that they were both able to spoiler[embarrass the other with darling talk], it seemed more two way street than the usual one way thing. It looks like Harutora failed as his explanation made her feel stupid for feeling sorry, and probably jealous too. The scene up till the was cute ...
According to the official players guide, its merely Try the Battle Subway 100 times or more. It doesnt say anything about winning 100 times in a row...
Four episodes into Season 1, watching via On Demand. Interesting and intriguing story about a socially awkward computer hacker living on the margins of...
(Quote) Or Krypton was never destroyed so she never left. Or it was destroyed and she died there. Or she died on the journey to Earth.
(Quote) Or Krypton was never destroyed so she never left. Or it was destroyed and she died there. Or she died on the journey to Earth.