Blood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16 to 25 h to 5-6 h, transforming patient care. To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared . A total of 2196 antimicrobial susceptibility test results (AST) were performed: 1863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and
article{5cf566a8-21ef-4c37-927c-b4548bf4fe7f, abstract = {Objectives: The aim of this study was to establish wild-type MIC distributions of first-line drugs for Mycobacterium tuberculosis, as well as to explore the usefulness of such distributions when setting clinical breakpoints. Methods: We determined the MICs of rifampicin, isonlazid and ethambutol for M. tuberculosis using a Middlebrook 7H10 dilution method for 90 consecutive clinical isolates, 8 resistant strains and 16 isolates from the WHO proficiency test panel. M. tuberculosis H37Rv was used for quality control and susceptibility results using 7H10 were compared with the results obtained with BACTEC460. Results: The agreement with BACTEC460 was very high for isonlazid (99.1%) and rifampicin (99.1%) but lower for ethambutol (94.7%). Intra- and inter-assay variation was below one MIC dilution. The MIC distributions for isoniazid and rifampicin provided a clear separation between susceptible and resistant strains. Regarding ethambutol, ...
Both the CLSI agar dilution method and Trek Sensititre broth microdilution panel for Streptococcus pneumoniae antimicrobial susceptibility testing were evaluated against the reference CLSI broth microdilution method using the most recently published CLSI breakpoints. While agar dilution was not an optimal method, the commercial panel appeared to be an acceptable method, with minor errors encountered for ceftriaxone, penicillin, and meropenem. ...
BioAssay record AID 447039 submitted by ChEMBL: Antibacterial activity against Escherichia coli ATCC 11230 after 24 hrs by agar disk diffusion assay.
The Clinical Laboratory Standards Institute lowered the fluoroquinolone minimum inhibitory concentration (MIC) susceptibility breakpoints for Enterobacteriaceae and glucose non-fermenting Gram-negative bacilli in January 2019. This retrospective cohort study describes the impact of this reappraisal on ciprofloxacin susceptibility overall and in patients with risk factors for antimicrobial resistance. Gram-negative bloodstream isolates collected from hospitalized adults at Prisma Health-Midlands hospitals in South Carolina, USA, from January 2010 to December 2014 were included. Matched pairs mean difference (MD) with 95% confidence intervals (CI) were calculated to examine the change in ciprofloxacin susceptibility after MIC breakpoint reappraisal. Susceptibility of Enterobacteriaceae to ciprofloxacin declined by 5.2% (95% CI: −6.6, −3.8; p < 0.001) after reappraisal. The largest impact was demonstrated among Pseudomonas aeruginosa bloodstream isolates (MD −7.8, 95% CI: −14.6,
BioAssay record AID 572901 submitted by ChEMBL: Antibacterial activity against blaVIM-2-positive Pseudomonas aeruginosa by CLSI broth microdilution method.
TY - JOUR. T1 - Evaluation of the e test system versus a microtitre broth method for antifungal susceptibility testing of yeasts against fluconazole and itraconazole. AU - Colombo, Arnaldo L.. AU - Barchiesi, Francesco. AU - Mcgough, Deanna A.. AU - Fothergill, Annette W.. AU - Rinaldi, Michael G.. PY - 1995/7/1. Y1 - 1995/7/1. N2 - The E test strip (AB Biodisk, Solna, Sweden) as an antimicrobial susceptibility testing method is a new and promising tool with broad application in microbiology. This method is less labour intensive than broth dilution methods and may be useful for testing individual clinical isolates.In contrast to several publications comparing E test antibacterial strips with NCCLS reference methods, there is littleinformation about the performance of E test antifungal strips. This study compared fluconazole and itraconazole MICs obtained by E test with a microbroth dilution method performed to NCCLS guidelines.Fluconazole and itraconazole E test results exhibited good ...
The Australian Group on Antimicrobial Resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2013 survey focussed for the first time on blood stream infections. Four thousand nine hundred and fifty-eight Enterobacteriaceae species were tested using commercial automated methods (Vitek® 2, BioMérieux; Phoenix™, BD). The results were analysed using Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints (January 2014). Of the key resistances, non-susceptibility to the third-generation cephalosporin, ceftriaxone, was found in 7.5%/7.5% (CLSI/EUCAST criteria respectively) of Escherichia coli; 6.3%/6.3% of Klebsiella pneumoniae, and 7.4%/7.4% of K. oxytoca. Non-susceptibility rates to ciprofloxacin were 10.3%/11.3% for E. coli, 4.6%/7.5% for K. pneumoniae, 0.6%/0.6% for K. oxytoca, and 3.6%/6.1% in Enterobacter cloacae. ...
Mueller and Hinton developed Mueller Hinton Agar in 1941 to be a protein free medium for isolating pathogenic strains of Neisseria . (3) It was found that Mueller Hinton Agar was useful in identifying sulfonimide-resistant and responsive strains of gonococci. (3) Additionally, in recent times this media has been used in standardized antimicrobial disk susceptibility testing, as described by Bauer, Kirby, et al. (1) Barry and Fay investigated the effects of altering the depth of plated Mueller Hinton Agar on disk diffusion testing, and determined a standardized depth of approximately four millimeters to be sufficient. (2) In 1970 Dewees, et al., studied the effect of storage on Mueller Hinton Agar plates used for antimicrobial disk diffusion zone sizes. Their findings indicated commercially manufactured Mueller Hinton Agar plates were suitable for use in routine susceptibility testing. (6) Mueller Hinton Agar with Lysed Horse Blood is a modification to the traditional Mueller Hinton Agar ...
Here, we present a protocol for determination of dalbavancin susceptibility of clinically relevant Gram-positive bacteria using a broth ...
Screening and enumeration of antimicrobial resistant Escherichia coli directly from samples is needed to identify emerging resistant clones and obtain quantitative data for risk assessment. Aim of this study was to evaluate the performance of 3M™ Petrifilm™ Select E. coli Count Plate (SEC plate) supplemented with antimicrobials to discriminate antimicrobial-resistant and non-resistant E. coli. A range of E. coli isolates were tested by agar dilution method comparing the Minimal Inhibitory Concentration (MIC) for eight antimicrobials obtained by Mueller-Hinton II agar, MacConkey agar and SEC plates. Kappa statistics was used to assess the levels of agreement when classifying strains as resistant, intermediate or susceptible. SEC plate showed that 74% of all strains agreed within ± 1 log2 dilution when comparing MICs with Mueller-Hinton II media. High agreement levels were found for gentamicin, ampicillin, chloramphenicol and cefotaxime, resulting in a kappa value of 0.9 and 100% agreement within ±
TY - JOUR. T1 - Effects of systemic administration of sitafloxacin on subgingival microflora and antimicrobial susceptibility profile in acute periodontal lesions. AU - Tomita, Sachiyo. AU - Kasai, Shunsuke. AU - Ihara, Yuichiro. AU - Imamura, Kentaro. AU - Kita, Daichi. AU - Ota, Koki. AU - Kinumatsu, Takashi. AU - Nakagawa, Taneaki. AU - Saito, Atsushi. PY - 2014/6. Y1 - 2014/6. N2 - The aim of this study was to assess the effect(s) of systemic administration of sitafloxacin on subgingival microbial profiles of acute periodontal lesions. Antimicrobial susceptibility of clinical isolates was also investigated. Patients with acute phases of chronic periodontitis were subjected to clinical examination and microbiological assessment of their subgingival plaque samples by culture technique. Sitafloxacin was then administered (100mg/day for 5 days) systemically. The clinical and microbiological examinations were repeated 6-8 days after administration. Susceptibilities of clinical isolates to various ...
Biofilm production and antibiotic susceptibility profile of Escherichia coli isolates from HIV and AIDS patients in the Limpopo Province
A four-center study in which a total of 1,082 recent clinical isolates of members of the family Enterobacteriaceae and Pseudomonas aeruginosa were examined versus 11 antimicrobial agents with the bioMérieux Vitek susceptibility test system (Hazelwood, Mo.) and the GNS-F6 card was conducted. In addition, a challenge set consisting of the same 200 organisms was examined in each of the four participating laboratories. Results obtained with the Vitek system were compared to MICs determined by a standardized broth microdilution method. For purposes of comparison, susceptibility categories (susceptible, intermediate, or resistant) were assigned on the basis of the results of both methods. The result of the broth microdilution test was considered definitive. The total category error rate with the Vitek system and the recent clinical isolates (11,902 organism-antimicrobial comparisons) was 4.5%, i.e., 1.7% very major errors, 0.9% major errors, and 1.9% minor errors. The total category error rate calculated
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The Kirb-Bauer Disk Diffusion Technique was performed on 2 different Mueller-Hinton Agar plates. One plate had Escherichia Coli spread on it and the other plate has Staphylococcus Epidermidis spread on it. Both plates had.
TY - JOUR. T1 - Evaluation of usefulness and PK-PD analysis of meropenem in children with various infections. AU - Sato, Yoshitake. AU - Sandoh, Mitsuru. AU - Hanaki, Hideaki. AU - Suzuki, Yumiko. AU - Yoshida, Mikinobu. AU - Kizu, Junko. PY - 2012/5. Y1 - 2012/5. N2 - We conducted a study of meropenem(MEPM) in a total of 29 children with various infections (moderate to severe pneumonia in 25, upper respiratory infection in 3, and urinary tract infection in 1) to demonstrate the efficacy and safety and assess the relationship between the time above the minimal inhibitory concentration (MIC) (T , MIC), calculated based on the pharmacokinetic simulation analysis using blood concentration data from subjects, and the clinical response. In accordance with the package insert in Japan, MEPM was administered at a dose of 20 mg/kg three times daily, the highest dose in the usual dosage range. Each dose was administered by infusion over 30 minutes. The minimum inhibitory concentration (MIC) values of MEPM ...
Summary. This Health Advisory describes the identification of emerging Shigella strains with elevated minimum inhibitory concentration values for ciprofloxacin and outlines new recommendations for clinical diagnosis, management, and reporting, as well as new recommendations for laboratories and public health officials. Current interpretive criteria provided by the Clinical and Laboratory Standards Institute (CLSI) categorize these strains as susceptible to ciprofloxacin, which is a fluoroquinolone antibiotic and a key agent in the management of Shigella infections.. However, recent data from the Centers for Disease Control and Prevention (CDC) and state and local public health partners show that these strains often have a quinolone resistance gene that may lead to clinically significant reduced susceptibility to fluoroquinolone antibiotics. Clinicians treating patients with multidrug-resistant shigellosis for whom antibiotic treatment is indicated should avoid prescribing fluoroquinolones if the ...
Video articles in JoVE about microbiology include Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology, The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections, Aseptic Laboratory Techniques: Plating Methods, Whole Genome Sequencing of Candida Glabrata for Detection of Markers of Antifungal Drug Resistance, Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction, Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS, Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP), A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii, A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of
In the last 10 to 20 years, we have witnessed a dramatic increase in the proportion of bacterial pathogens resistant to multiple antimicrobial agents. Indeed, the driving force behind the increasing rates of resistance is ultimately the abuse and misuse of antimicrobial agents, whether inadequately administered to patients and livestock or unintentionally released into the environment. This issue is very important regarding the resistance towards quinolones, carbapenems, and third-generation cephalosporins. The latter relates to the increased prevalence of extended-spectrum β-lactamases among Enterobacterales. Surveillance studies of antimicrobial resistance and antibiotic consumption have drawn attention to this phenomenon and should be used to drive political campaigns to contain resistance [18, 19]. Ceftolozane/tazobactam (C/T) has been approved few years ago and represents a therapy option in particular infections associated with Gram-negative bacteria, including ESBL-producing isolates. ...
PURPOSE: Extended spectrum β-lactamase (ESBL) and AmpC β-lactamase are important mechanisms of betalactam resistance among Enterobacteriaceae . The ESBL confirmation test described by Clinical Laboratory Standards Institute (CLSI) is in routine use. This method fails to detect ESBL in the presence of AmpC. Therefore, we compared two different ESBL detection methods against the CLSI confirmatory test. MATERIALS AND METHODS: A total 200 consecutive clinical isolates of Enterobacteriaceae from various clinical samples were tested for ESBL production using (i) CLSI described phenotypic confirmatory test (PCT), (ii) boronic acid disk potentiation test and (iii) cefepime-CA disk potentiation method ...
and : Susceptibility Testing of 120 Isolates to Six Antimicrobial Agents Using Disk Diffusion (EUCAST), Etest, and Broth Microdilution Techniques
This project builds on an initial pump-priming project funded by EPSRC BristolBridge and subsequent funding (via the EBI, the Longitude Prize and many others) to develop a rapid antimicrobial susceptibility test to inform antibiotic prescribing. This new work will scope the parameters for the constraints for end-users, particularly GPs and other community health workers working in primary care and urgent care. A Knowledge Mobilisation approach will be taken to co-produce a stakeholder informed plan to outline the challenges of designing, prototyping and economically evaluating a novel SCFI antimicrobial resistance device to help combat AMR in community healthcare settings. The projects industry partner is Vitamica Ltd - a University of Bristol spin out company formed after the development of the SCFI technology. ...
Clinical and Laboratory Standards Institute (2008) Performance standards for antimicrobial disk and dilution susceptibility test for bacteria isolated from animals; approved standard, 3rd edn. In CLSI Document M31-A3. ed. Watts, J.L., Shryock, T.R., Apley, M., Bade, D.J., Brown, S.D., Gray, J.T., Heine, H., Hunter, R.P. et al. , pp. 32-35. Wayne, PA: Clinical and Laboratory Standards Institute ...
The aim of this study was to isolate and characterize psychrotrophic bacteria resistant to antibiotic and metals from cold samples of refrigerated spoiled food and ice. Two isolates named H and F were successfully isolated from samples incubated at 10°C and 4°C, respectively. Both isolates were able to grow at 4°C, 10°C, 20°C and 30°C and exhibited multidrug resistance to antibiotics (chloramphenicol, ampicillin, tetracycline and kanamycin). Bacteria H and F were then further tested for their resistance to metal ions such as Cd2+, Zn2+, Cr 6+ and As 3+ at concentrations ranging from 0-10 mM. The results of the test revealed that the minimum inhibitory concentration (MIC) for isolate H was determined at 2 mM for all the metal ions. By comparison, isolate F was highly resistant to Cr6+, Cd2+ and Zn2+ with MIC of 10 mM for these cations, while MIC for As3+ was 4 mM. The results of comparing 16S rRNA gens demonstrated that isolate F has 98% identity match with Bacillus sp. This strain could be ...
Purpose: The Antibiotic Resistance Monitoring in Ocular micRoorganisms (ARMOR) study was initiated in 2009 to survey resistance levels among ocular pathogens on a nationwide scale. Here we report the complete study results for 2013 compared to preliminary 2014 data.. Methods: Isolates of Streptococcus pneumoniae, Staphylococcus aureus, coagulase-negative staphylococci (CoNS), Pseudomonas aeruginosa, and Haemophilus influenzae were subjected to antibiotic susceptibility testing. In 2013, 543 isolates were collected from 22 sites; 140 isolates have been collected from 7 sites to date in 2014. Minimum inhibitory concentrations were determined by broth microdilution for up to 16 representative antibiotics per CLSI methods. Systemic breakpoints (where available) were used to categorize isolates as susceptible or non-susceptible (intermediate and resistant).. Results: With the exception of a fluoroquinolone-resistant isolate and an azithromycin-resistant isolate observed in 2014, all H. influenzae ...
Read the latest articles on clinical and laboratory standards from the Clinical & Laboratory Standards Institute (CLSI). Find great content from CLSI.
Read the latest articles on clinical and laboratory standards from the Clinical & Laboratory Standards Institute (CLSI). Find great content from CLSI.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Next Generation PHENOTYPIC SUSCEPTIBILITY TESTING Assays. ​FASTinov is a R&D intensive Startup with a patented disruptive technology to perform fast and reliable antimicrobial susceptibility tests in acute care settings. Context : The global emergence and spread of antimicrobial resistance is a major medical and economic problem . When an infection is diagnosed, the physician is required to start an empiric larg e spectrum therapy, since current comprehensive and proven susceptibility profile methods require, at least, 48h. FASTinov approach : In order to support clinical decisions with a fast turnaround time, FASTinov offers a unique time-saving and comprehensive solution starting directly from positive blood cultures. FASTinov innovative approach allows for the determination of the susceptibility phenotype in 2 hours, compared with +48 hours needed for current standard methods. Disruptive Technology : FAST patented methodology (Flow Cytometry Antimicrobial Susceptibility Test) fills a gap ...
Florin Anghelina, Ovidiu Zlatian, Mircea Sorin Ciolofan, Daniela Calina, Anca Oana Docea, Razvan Hainarosie, Octavian Dragos Palade, Liliana Anghelina, Cristian Dragos Stefanescu, Laura Mazilu, Andra Iulia Suceveanu, Catalina Pietrosanu, Andrei Osman - Bacteriological Profiles and Antibiotic Susceptibility Patterns in Complicated Bacterial Infections of the Ears, Nose and Throat
Antibiotic resistance is the ability of bacteria to survive exposure to antibiotics that were designed to kill them or inhibit their growth This experiment aimed to answer the question if chronic low-grade usage of amoxicillin, ciprofloxacin, or trimethoprim promoted antibiotic-resistant Escherichia coli over time. This in vitro study tested if the concentration of antibiotics and the type of antibiotic affected the minimum inhibitory concentration (MIC) required for each antibiotic to inhibit bacterial growth. To find these measurements a MIC assay was used, and the bacterial concentrations in each well were recorded and compared. The expectation of this study was that as the bacteria were treated with high concentration antibiotics for a longer amount of time, a lower bacterial growth would be observed. A 2-Factor ANOVA reported that in a full factorial model, there was not a significant interaction between antibiotic exposure and type of bacterial concentration (F= 1.597, df=2,12, p= 0.2428), ...
The Antimicrobial Index is comprised of literature-based minimum inhibitory concentration values collected from hundreds of scientific papers as well as other relevant antimicrobial potency data.
Challenging bacteria in spatial gradients of antibiotics leads to large increases in resistance through sequential adaptive steps across competing lineages (Fig. 1 and movie S1). We first set up the MEGA-plate with symmetric four-step gradients of trimethoprim (TMP) or ciprofloxacin (CPR) proceeding inward with order-of-magnitude increases in concentration per step [Fig. 1A; TMP: 0, 3, 30, 300, and 3000 × wild-type minimum inhibitory concentration (MIC); CPR: 0, 20, 200, 2000, and 20,000 × MIC] and inoculated the drug-free regions with Escherichia coli. Bacteria swim and spread until they reach a concentration in which they can no longer grow (TMP, Fig. 1C and movies S1 and S2; CPR, movie S3). As resistant mutants arise in the population, their descendants migrate into the next step of drug concentration and fan out (Fig. 1C, 88 hours). Adjacent mutant lineages exclude each other and compete for limited space, resulting in some lineages entirely blocking off growth of others (Fig. 1C). When ...
Innovative Grade US Origin Horse Whole Blood Lysed from Innovative Research was used in the following study: Mathematical modeling of the inoculum effect: six applicable models and the MIC advancement point concept Jessica R. Salas, Majid Jaberi-Douraki, Xuesong Wen, Victoriya V. Volkova FEMS Microbiology Letters 21 January 2020 …Antimicrobial treatment regimens against bacterial pathogens are designed using the drugs minimum inhibitory concentration (MIC) measured at the bacterial density of 5.7 log10(colonyforming units (CFU)/mL) in vitro. However, MIC changes with pathogen density, which varies among infectious diseases and during treatment. Incorporating this into treatment design requires realistic mathematical models of.... ...
Scattergram #2 -- Correlation of TMP/SMX MIC and zone diameters for S. pneumoniae. Horizontal and vertical lines represent MIC and zone diameter breakpoints. In this case, the isolates form a continuum, with the breakpoint for resistant and susceptible not being obvious. In this case one can not predict how the isolates which fall into the intermediate zone will behave in vivo. The breakpoints are set to maximize the predictive value of the test while minimizing errors (ie. it is preferable to call an isolate intermediate, than to incorrectly call it sensitive or resistant ...
Antimicrobial resistance (AMR) is one of the major threats to human and animal health worldwide, yet few high-throughput tools exist to analyse and predict the resistance of a bacterial isolate from sequencing data. Here we present a new tool, ARIBA, that identifies AMR-associated genes and single nucleotide polymorphisms directly from short reads, and generates detailed and customizable output. The accuracy and advantages of ARIBA over other tools are demonstrated on three datasets from Gram-positive and Gram-negative bacteria, with ARIBA outperforming existing methods.
Ashraf Mohamadkhani, Sima Besharat, Golnosh Gol-Jah Rad, Akbar Pour Dadash Asiabar, Gholamreza Roshandel, Akram Pourshams, Hossein Poustchi ...
The Antibiotics susceptibility plugin in BIONUMERICS automatically converts inhibition zone diameters and MIC values into SIR categories for antibiotic resistance typing.
Appropriate medium(a blood agar plate)and incubated overnight at 35C Inoculate a tube that contains 2 ml of saline or Mueller-hinton broth With five or more colonies from the agar plate and adjust turbidity to Match a McFarland standard(approximately 10 CFU/ml) Transfer 0.1 ml of the turbid broth into 10 ml of Mueller-Hinton Broth,inoculate in shaking water bath or equivalent at 35C until Turbid(5 to 6 hours for rapid growth).The relevant control organism Should be inoculated into 3 ml of broth and incubated without shaking Until turbid.This represents a midexponential phase of growth. Prepare twofold serial dilutions of the antibiotic in 2 ml of Mueller-Hinton broth.Use acid washed borosilicated glass tubes. Standardise the inoculum of the patients organism and the ...
Rating: 4.8/5. Many top tutors available in McFarland, WI and surrounding suburbs for award-winning private in-home or online tutoring.
Fully integrated within BD Phoenix panels, the new BD Phoenix CPO detect test enables laboratories to offer a more comprehensive antimicrobial susceptibility test profile while improving laboratory process efficiency.
HayrehSS,PeвerJ,ZimmermanMBMorphologicchangesinchronichigh-pressureexperi- mental glaucoma in rhesus monkeys. Prostate Cancer Trialistsв Collaborative Group. Fig. 4 In Vitro Antimicrobial Susceptibility Testing Therapy keflx active pertussis keflex sibo prophylaxis of close contacts is considered to minimize transmission (6).
The first of the studies is RAPIDS-GN (ClinicalTrials.gov # NCT03218397), a randomized controlled trial conducted at Mayo Clinic and UCLA. RAPIDS-GN focused on Gram-negative bacteremia and randomized patients to be tested by the BC kit or legacy methods. The study met the primary endpoint of therapy optimization, reporting time to first Gram-negative antibiotic change a full 24 hours sooner than legacy methods (17.4 hours vs. 42.1 hours, p,0.01) and time to any antibiotic change 6 hours sooner (8.6 hours vs. 14.9 hours, p=0.02). ...
Stella-Jones, Inc., wholly-owned subsidiary McFarland Cascade Holdings, Inc., has signed a non-binding letter of intent to acquire the shares of Lufkin Creosoting Co., Inc.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Our shop retails Kirby`s Dream Land Terrarium Collection Kirby`s Adventure (Set of 8) (Anime Toy) Kirby`s Dream Land Re-Ment 203973 Anime Goods on the Web.
Development of a superior cleaning cloth with constant and continuous antibacterial effect, ensuring that no bacteria is left in the cloth and making bacterial re-growth impossible, without need for special and time-consuming hygiene procedures. This will be accomplished by a unique technology with antibacterial agent integrated into the microfiber of the cloth. The advances in this technology will allow significant reduction of cross-contamination and bacterial regrowth. Infectious diseases arising in the home setting are a significant concern. ...
Staphylococcus aureus (S. aureus) is a major pathogen associated with serious community and hospital-acquired infections. Methicillin resistant S. aureus (MRSA) is now endemic in India. Methicillin-resistant Staphylococcus aureus (MRSA) has recently emerged as a major nosocomial pathogen worldwide with a significant morbidity and mortality. Early detection of emerging trends in antimicrobial resistance may facilitate implementation of effective control measures. The present study thus attempts to characterize the MRSA isolates and explore the antibiotic susceptibility pattern of MRSA isolated from wound samples at a tertiary care hospital, Central India. The present study was conducted to characterize 120 MRSA isolates, isolated from wound samples and their antibiotic susceptibility patterns. MRSA was identified by oxacillin disc diffusion test, cefoxitin disc diffusion test and resistance to oxacillin by the Minimum inhibitory concentration (MIC) method. Study also detected inducible clindamycin
Effects of various antimicrobial agents on multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells
Abstract:. To compare conventional phenotypic methods for the detection of methicillin in Staphylococci aureus in routine laboratory practice with reference to an established molecular method. This study was conducted on a selection of 30 isolates of methicillin resistant Staphylococci aureus (MRSA) from clinical specimens. The Kirby- Bauer disc diffusion tests and oxacillin screen ager method were performed on all isolates using the presence of penicillin binding protein (PBP2a) as the reference standard. A commercial latex agglutination test (Oxoid, UK) was assessed for the detection of penicillin binding protein 2a (PBP2a), the Mec A gene product. Twenty of 30 isolates were positive to PBP2a and concomitant manifest resistance to (oxacillin) was confirmed using Kirby-Bauer diffusion test. All the thirty isolates were resistant using disk diffusion method. The specificity and sensitivity of this method, in comparison with PBP2a was 100% in our examined strains, whereas the specificity and ...
Acinetobacter baumannii is an important opportunistic pathogen, responsible for approximately 10% of all gram-negative nosocomial infection. The aim of this study was to determine aminoglycoside and quinolone resistance genes and their antimicrobial susceptibility profile in the clinically A. baumannii. In this cross-sectional study, a total of 100 nonduplicative A. baumannii isolates were collected from different clinical samples. Antimicrobial susceptibility test was performed by disk diffusion method. QnrA, anrB, qnrS, aac(3)-IIa, and aac(6′)-Ib genes were identified using PCR method. The results of antibiotic susceptibility test showed that polymyxin B was the most effective antimicrobial against A. baumannii. 97%, 95% and 82% of isolates were resistant to cefepime, ceftriaxone, and amikacin, respectively. The molecular distribution of aac(3)-IIa, aac(6′)-Ib, and qnrA genes were 45%, 50%, and 50% of isolates, respectively. However, qnrB and qnrS genes could not be detected in any strain. ...
Define antibiotic sensitivity test. antibiotic sensitivity test synonyms, antibiotic sensitivity test pronunciation, antibiotic sensitivity test translation, English dictionary definition of antibiotic sensitivity test. n. A substance, such as penicillin or erythromycin, produced by or derived from certain microorganisms, including fungi and bacteria, that can destroy or...
TY - JOUR. T1 - Invasive Candida guilliermondii infection. T2 - In vitro susceptibility studies and molecular analysis. AU - Vazquez, J. A.. AU - Lundstrom, T.. AU - Dembry, L.. AU - Chandrasekar, P.. AU - Boikov, D.. AU - Perri, M. B.. AU - Zervos, M. J.. PY - 1995/12/1. Y1 - 1995/12/1. N2 - Candida guilliermondii is rarely isolated from humans. We describe a case of disseminated C. guilliermondii with associated purulent pericarditis, despite high-dose amphotericin B (AmB), in a 19-year-old female with aplastic anemia who underwent BMT. In vitro susceptibility studies of the 13 clinical isolates, two control strains and one environmental isolate revealed a minimum inhibitory concentration (MIC) range of (0.19-1.56 μg/ml) for AmB and (1.25-10 μg/ml) for fluconazole. Pulsed-field gradient gel electrophoresis was performed to evaluate possible similarities between strains. This case is significant for several reasons, the high degree and prolonged duration of fungemia despite high-dose AmB and ...
The continuous spread of penicillin-resistant pneumococci represents a permanent threat in the treatment of pneumococcal infections, especially when strains show additional resistance to quinolones. The main objective of this study was to determine a treatment modality impeding the emergence of quinolone resistance. Exposure of a penicillin-resistant pneumococcus to increasing concentrations of trovafloxacin or ciprofloxacin selected for mutants resistant to these drugs. In the presence of sub-inhibitory concentrations of vancomycin, development of trovafloxacin-resistance and high-level ciprofloxacin-resistance were prevented. Considering the risk of quinolone-resistance in pneumococci, the observation might be of clinical importance.
Background: Urinary tract infection (UTI) is one of the most common infections among pregnant women and if untreated could lead to serious complications.. Objective: This work was carried out to determine the prevalence rate of Enterobacteriaceae uropathogens and their antibiotics susceptibility profile among pregnant women attending two ante-natal clinics in Ilorin, Nigeria.. Materials and Methods: A total of 111 pregnant women between the ages of 18-50 attending ante-natal clinic at University of Ilorin Teaching Hospital and Civil Service Hospital, Ilorin participated in the study. Mid-stream urine samples were collected and cultured on Cysteine-Lactose-Electrolyte Deficient agar. Presence of significant bacteriuria (, 105 cfu/mL) was determined using the plate count method. Antibiotic susceptibility testing was done using Kirby-Bauer disk diffusion technique.. Results: Of the 111 urine samples collected, 27.9% of them were found positive on culture. Among the isolated organisms, Klebsiella ...
This study centred on the assessment of Suyas ready-to - eat (RTE) bacteriology and antibiotic susceptibility profile. Samples were bought randomly from manufacturing sources and vendors, and a number of putative bacterial pathogens were isolated according to traditional microbiological protocols. Roasted and sold Suya aerobic plate counts (APCs) ranged from 2.4×104-1.39×105 CFUg-1 while total coliform counts (TCCs) ranged from 1.5×104-6.2×104 CFU / g. Ten (10) bacterial species, including six (6) Gram-positive and four (4) Gram-negative bacteria, were described in total. Staphylococcus aureus (20%), Escherichia coli and Micrococcus luteus (16%), respectively, were the most commonly isolated bacteria. Results of the antibiotic susceptibility test showed that Ciprofloxacin and Levofloxacin had the highest inhibition zones ranging from 21-41 mm (among others) for Gram positive and negative bacteria with high sensitivity and broad spectrum levels. Action. Action In comparison, Klebsiella ...
TY - JOUR. T1 - In vitro activity of gemifloxacin against recent clinical isolates of bacteria in Korea.. AU - Yong, Dong Eun. AU - Cheong, Hee Jin. AU - Kim, Yang Soo. AU - Park, Yeon Joon. AU - Kim, Woo Joo. AU - Woo, Jun Hee. AU - Lee, Kyung Won. AU - Kang, Moon Won. AU - Choo, Youn Sung. PY - 2002/12. Y1 - 2002/12. N2 - Gemifloxacin is an enhanced-affinity fluoroquinolone with broad-spectrum antibacterial activity. In Korea, resistant bacteria are relatively more prevalent than in other industrialized countries. In this study, we studied the in vitro activities of gemifloxacin, gatifloxacin, moxifloxacin, levofloxacin, ciprofloxacin, and other commonly used antimicrobial agents against 1,689 bacterial strains isolated at four Korean university hospitals during 1999-2000. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method of National Committee for Clinical Laboratory Standards. Gemifloxacin had the lowest MICs for the respiratory pathogens: 90% of ...
GISP is a CDC-supported sentinel surveillance system that has monitored N. gonorrhoeae antimicrobial susceptibilities since 1986, and is the only source in the United States of national and regional N. gonorrhoeae antimicrobial susceptibility data. During September-December 2011, CDC and five external GISP principal investigators, each with N. gonorrhoeae-specific expertise in surveillance, antimicrobial resistance, treatment, and antimicrobial susceptibility testing, reviewed antimicrobial susceptibility trends in GISP through August 2011 to determine whether to update CDCs current recommendations (2) for treatment of uncomplicated gonorrhea. Each month, the first 25 gonococcal urethral isolates collected from men attending participating STD clinics (approximately 6,000 isolates each year) were submitted for antimicrobial susceptibility testing. The minimum inhibitory concentration (MIC), the lowest antimicrobial concentration that inhibits visible bacterial growth in the laboratory, is used ...
Abstract:. Introduction: Streptococcus pneumoniae (S.pneumoniae) is an important cause of morbidity and mortality in people of extreme age. Drug resistant pneumococci are emerging and causing treatment failure. In the present study we evaluated Polymerase Chain Reaction (PCR) for rapid detection of S. pneumoniae directly from the clinical samples and antimicrobial susceptibilities of the culture isolates Material & methods: A total of 90 clinical samples from sterile sites were cultured. Direct detection of S.pneumoniae from the samples was done using PCR. The isolates were tested for antimicrobial susceptibility by disc diffusion method and Minimum Inhibitory Concentration (MIC) of penicillin was determined by microbroth dilution method. Results: Out of 90 samples 34 were positive for S. pneumoniae by PCR and 23 samples showed growth in culture. Of these 23 isolates, disc diffusion method showed that 61%, 0%, 13%, 35%, 83%, 4.3%, 0% of the isolates were resistant to penicillin, erythromycin, ...
This research work investigated the influence of biofield treatment on Enterobacter cloacae (ATCC 13047) against antimicrobial susceptibility. Two sets of ATCC samples were taken in this experiment and denoted as A and B. ATCC A sample was revived and divided into two parts Gr. I (control) and Gr. II (revived); likewise, ATCC B was labeled as Gr. III (lyophilized). Group II and III were given with biofield treatment. The control and treatment groups of E. cloacae cells were tested with respect to antimicrobial susceptibility, biochemical reactions pattern and biotype number. The result showed significant decrease in the minimum inhibitory concentration (MIC) value of aztreonam and ceftazidime (≤ 8 μg/mL), as compared to control group (≥ 16 μg/mL). It was observed that 9% reaction was altered in the treated groups with respect to control out of the 33 biochemical reactions. Moreover, biotype number of this organism was substantially changed in group II (7731 7376) and group III (7710 3176) ...
Here, we used semi-quantitative radial diffusion assay and broth microdilution assay to evaluate susceptibility of a number of multi-drug resistant (MDR) clinical isolates to the MRJP1-contaning honey glycoproteins. The MDR bacterial strains comprised three methicillin-resistant Staphylococcus aureus (MRSA), four Pseudomonas aeruginosa, two Klebsiella pneumoniae, two vancomycin-resistant Enterococci (VRE), and five ESBL identified as one Proteus mirabilis, three E. coli, and one E. coli NDM. Their resistance to different classes of antibiotics was confirmed using automated system Vitek 2. MDR isolates differed in their susceptibility to glps with MIC90 values ranging from 4.8 μg/ml against B. subtilis to 14.4 μg/ml against ESBL K. pneumoniae, Klebsiella spp. ESBL and E. coli and up to 33 μg/ml against highly resistant strains of P. aeruginosa ...
Recent years have seen a dramatic rise in the incidence and severity of cases of human salmonellosis, in addition, multidrug resistant strains have arisen. the objective of this study as to characterize the antimicrobial susceptibility profile of Salmonella spp. clinical isolates collected from Latin American medical centers as part of the SENTRY Antimicrobial Surveillance System. A total of 144 bloodstream Salmonella spp. isolates were collected between 1997 and 2000. the susceptibility to diverse antimicrobial agents was tested by broth microdilution techniques according to the NCCLS recommendations. the Salmonella spp. strains were more frequently collected from adult patients (67.0%; 21-60 years) and isolated from Chile (28.5%) , Brazil (25.0%) , Mexico , Colombia ( 11,8%). Ampicillin (MIC50, 1 mug/ml) showed good in vitro activity (92.4% susceptibility). Meropenem (MIC50. 0.06 mug/ml) and gatifloxacin (MIC50, 0.03 mug/ml) were the most Potent compounds against the Salmonella spp. isolates ( ...
TY - JOUR. T1 - Antimicrobial susceptibility profiles of Gram-negative bacilli isolated from patients with hepatobiliary infections in Taiwan. T2 - Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2006-2010. AU - Toh, Han Siong. AU - Chuang, Yin Ching. AU - Huang, Chi Chang. AU - Lee, Yu Lin. AU - Liu, Yuag Meng. AU - Ho, Cheng Mao. AU - Lu, Po Liang. AU - Liu, Chun Eng. AU - Chen, Yen Hsu. AU - Wang, Jen Hsien. AU - Ko, Wen Chien. AU - Yu, Kwok Woon. AU - Liu, Yung Ching. AU - Chen, Yao Shen. AU - Tang, Hung Jen. AU - Hsueh, Po Ren. PY - 2012/6. Y1 - 2012/6. N2 - We investigated the trends in antimicrobial resistance among species of Gram-negative bacilli isolated from patients with hepatobiliary tract infections in Taiwan during the period 2006-2010 as part of the Study for Monitoring Antimicrobial Resistance Trends (SMART). During the study period, 1032 isolates of Gram-negative bacilli that had been collected from patients with hepatobiliary infections were ...
The in vitro activity of tigecycline (former GAR-936), a new semisynthetic tetracycline, was evaluated in comparison with tetracycline and other antimicrobial agents. MATERIAL AND METHODS: A total of 1,326 contemporary clinical isolates collected from the Latin American region were collected in 2000-2002 period and tested with microdilution broth according to the CLSI guidelines. The bacterial pathogens evaluated included Staphylococcus aureus (505), Streptococcus pneumoniae (269), coagulase-negative staphylococci (CoNS; 227), Haemophilus influenzae (129), Enterococcus spp. (80), Moraxella catarrhalis (54), beta-haemolytic streptococci (28), viridans group streptococci (26), and Neisseria meningitidis (8) RESULTS:Tigecycline demonstrated excellent activity against all Gram-positive cocci, with 90% of penicillin-resistant S. pneumoniae strains being inhibited at 0.12 µg/mL, while the same isolates had an MIC90 of , 16 µg/mL for tetracycline. All Enterococcus spp. were inhibited at 0.25 µg/mL ...
Background & Objectives: Due to increase in  bacterial drug resistance, discovering new antibacterial compounds is really important. The objective of this study is to evaluate the phenol compounds effect on antibacterial activity of herbal extracts of Fasa-Fars province in vitro. Materials & Methods: The antibacterial activity of 26 plants was ...
Pyogenic infections are one of the most common clinical complications following surgery and trauma. Prompt antimicrobial therapy is needed to reduce the morbidity of these infections. Rationale use of antibiotics is the need of the hour to curb the rising antimicrobial resistance. A retrospective study was undertaken to identify the microbial profile with their antibiogram among the pus culture isolates in a teaching tertiary care hospital from January 2017 to June 2017.504 pus culture samples processed at the microbiology lab were analyzed. The isolates from positive pus culture were identified by standard protocols and antimicrobial susceptibility patterns were identified by CLSI guidelines. Positive pus cultures were obtained in 59.92% (302/504). Gram positive bacteria accounted for 31.9 % (97), Gram negative bacteria 67.12% (204) and Candida spp 0.99% (3) with predominance of S. aureus 26.32% (80) followed by E. coli 13.82% (42) and K. pneumoniae 13.49% (41). 17.5% of S. aureus was MRSA and 100%
Basically, antibiotic compounds play an important role of effector molecules in their natural environments, then their mechanism of action could be seen as using their specific targets as signal relays. For example, tetracyclines binding to the ribosome cause a breakdown in translation that ultimately lead to cell death at high concentrations; but at sub-inhibitory concentrations, the binding to the ribosomes causes a temporary stall in translation that leads to an mRNA build up thereby stabilizing mRNA transcript concentrations in target cells. (Fajardo et al., 2008). Therefore, the action of antibiotic is actually an interesting way of using ribosomes, DNA, RNA, carbohydrates (peptidoglycan) as signal effector receptors and transducers .If antibiotic compounds play the role of effector molecules in their natural environments, then their mechanism of action could be seen as using their specific targets as signal relays. An example, is when tetracyclines binding to the ribosome cause a breakdown ...
Target modification and reduced drug accumulation are the main resistance mechanisms against fluoroquinolone antibiotics in Pseudomonas aeruginosa. We performed a genotypic characterization of three major Mex multidrug efflux pumps (MexAB-OprM, MexXY-OprM and MexCD-OprJ) in ciprofloxacin resistant clinical isolates of P. aeru-ginosa, collected from Tehran, Iran this was followed by sequencing and ...
Abstract : Study background: Staphylococci are widespread in nature, mainly found on the skin and mucous membranes. Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of biofield treatment on antimicrobial susceptibility pattern and biochemical characteristics of S. aureus (ATCC 25923). Methods: S. aureus cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 25923) number and stored according to the recommended storage protocols until needed for experiments. Revived and lyophilized state of ATCC strains of S. aureus were selected for the study. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. aureus were subjected to Mr. Trivedis biofield treatment. Revived treated cells were assessed on day 5 and day 10 while lyophilized treated cells on day 10 only. After biofield treatment both treated cells ...
Abstract : Study background: Staphylococci are widespread in nature, mainly found on the skin and mucous membranes. Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of biofield treatment on antimicrobial susceptibility pattern and biochemical characteristics of S. aureus (ATCC 25923). Methods: S. aureus cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 25923) number and stored according to the recommended storage protocols until needed for experiments. Revived and lyophilized state of ATCC strains of S. aureus were selected for the study. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. aureus were subjected to Mr. Trivedis biofield treatment. Revived treated cells were assessed on day 5 and day 10 while lyophilized treated cells on day 10 only. After biofield treatment both treated cells ...
In addition, data collected in the participating Brazilian centers were analyzed separately from the data of the entire Latin American region (including Brazil). Minimum antimicrobial concentrations that inhibits 50% and 90% of the organism collection (MIC50 and MIC90, respectively) were presented only for the data of the entire region. Isolates collected from bloodstream infections and from lower respiratory tract infections (mainly pneumonia) in hospitalized patients were also analyzed separately (Tables 3, 5, 7 and 9). Two groups of pathogens, streptococci and extended-spectrum (ESBL)-producing strains, were analyzed separately due to their importance in community-acquired and nosocomial-acquired infections, respectively. Table 10 presents the antimicrobial susceptibility of streptococcal species, while Table 11 presents the antimicrobial susceptibility of ESBL-producing E. coli and Klebsiella pneumoniae. Discussion. We made a comprehensive analysis of the Latin American SENTRY Program data ...
Pseudomonas aeruginosa is a major cause of nosocomial infections with high mortality rates. The organism is highly resistant to most classes of drugs used and can develop resistance during treatment. One of the resistance mechanisms of P. aeruginosais is Metallo-β-Lactamase (MBL) production. MBL producing P. aeruginosa is a major health concern given its resistance to almost all available drugs. The prevalence of this resistant strain is unknown since there is no standardized method for testing MBL production. This was a laboratory based cross-sectional prospective study that was carried out from September 2015 to March 2016 at Kenyatta National Hospital. Ninety-nine isolates of P. aeruginosa were collected during the period and tested for antimicrobial susceptibility and isolates found to be resistant to imipenem tested for MBL production. The results indicated high resistance of P. aeruginosa to commonly used drugs. Of the isolates tested 69.7% were resistant to piperacillin, 63.6% were resistant to
The purpose of this study was to evaluate the antibiotic susceptibility profile of Pseudomonas aeruginosa isolated from different animal species with septic ocular surface disease. Sixteen strains of P. aeruginosa were isolated from different species of animals (dog, cat, horse, penguin and brown bear) with ocular surface diseases such as conjunctivitis, keratocojnuctivits sicca and ulcerative keratitis. These isolates were tested against 11 different antimicrobials agents using the Kirby-Bauer disk-diffusion method. Minimum inhibitory concentrations (MICs) were determined using E-tests for two antibiotics (tobramycin and ciprofloxacin) commonly used in veterinary ophthalmology practice. Imipenem was the most effective antibiotic, with 100% of the strains being susceptible, followed by amikacin (87.5%), gentamicin, norfloxacin, gatifloxacin and polymyxin (both with 81.5%of susceptibility). MIC90 of ciprofloxacin was 2 µg/ml and the values found ranged from 0.094 µg/ml to 32 µg/ml. For tobramycin,
Objective To study antimicrobial resistance of Brucella melitenesis and instruct rational use of antimicrobial agents for clinic.Methods Bacteria were cultured and identified by Bact/Alert 3D and VITEK Ⅱ automicrobic system.Drug susceptibility was detected by E-test method.Results Four strains Brucella melitenesis were isolated from blood of 4 patients with fever and MIC of gentamicin,ampicillin and ciprofloxacin was(0.5)~(0.75 mg/L),(1.5)~(2.0 mg/L) and (4.0)~(8.0 mg/L) respectively.MIC of ceftazidime,amikacin and doxycycline were all above(8.0 mg/L).Conclusion The preferred drug for treating brucellosis is gentamicin or plus ampicillin or ciprofloxacin,or choosing antimicrobial agents according to antimicrobial susceptibility test results.
Active efflux of antibiotics preventing their accumulation to toxic intracellular concentrations contributes to clinically relevant multidrug resistance. Inhibition of active efflux potentiates antibiotic activity, indicating that efflux inhibitors could be used in combination with antibiotics to reverse drug resistance. Expression of ramA by Salmonella enterica serovar Typhimurium increases in response to efflux inhibition, irrespective of the mode of inhibition. We hypothesized that measuring ramA promoter activity could act as a reporter of efflux inhibition. A rapid, inexpensive, and high-throughput green fluorescent protein (GFP) screen to identify efflux inhibitors was developed, validated, and implemented. Two chemical compound libraries were screened for compounds that increased GFP production. Fifty of the compounds in the 1,200-compound Prestwick chemical library were identified as potential efflux inhibitors, including the previously characterized efflux inhibitors mefloquine and ...
Pharmacodynamic and mutant prevention properties of the fluoroquinolone pradofloxacin (PRA) were measured against a set of 17 Escherichia coli strains carrying no, one or two known mutations conferring reduced fluoroquinolone susceptibility. The strains included susceptible wild-types, isogenic constructed mutants, isogenic selected mutants and clinical isolates. The effectiveness of PRA was determined with regard to preventing the selection of resistant mutants, using static and changing concentrations of drug. Ciprofloxacin was used as a reference drug. Minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of PRA for the susceptible wild-type strains were in the range 0.012-0.016 mg/L and 0.2-0.3 mg/L, respectively, giving a mean +/- standard deviation mutant prevention index (MPI=MPC/MIC) of 17.7 +/- 1.1. The mean MPI PRA of the 14 mutant strains was 19.2 +/- 12, and the mean MPI across all 17 strains was 18.9 +/- 10.8. In an in vitro kinetic model in which PRA ...
Against streptococci other than pneumococci, the intrinsic potency of GAR-936, based on a comparison of MIC50s, was equivalent or slightly superior to that of minocycline. Median (modal) MICs for GAR-936 and minocycline were 0.06 (0.06) and 0.12 (0.12) μg/ml, respectively. However, all streptococcal isolates, including strains resistant to doxycycline or minocycline, were inhibited by GAR-936 at ≤0.25 μg/ml.. For pneumococci, the results shown in Table 1 were obtained by agar dilution. The activity of GAR-936 against 60 strains ofStreptococcus pneumoniae, half of which were not susceptible to penicillin, was also evaluated by microdilution. The MIC50 and MIC90 were 0.03 and 0.06 μg/ml. These were within 1 dilution of agar dilution results. Individually, microdilution results were 2 dilutions lower than the agar dilution results for 11 strains, 1 dilution lower for 27, equivalent for 13, and 1 dilution greater for 4. For S. pneumoniae ATCC 49619, the broth microdilution result was 1 dilution ...
New macrolide expands treatment options for bovine respiratory disease (BRD) in cattle.. The new antimicrobial tildipirosin is now available on veterinary microbroth dilution susceptibility plates, enabling veterinary microbiology laboratories to perform antimicrobial susceptibility testing on organisms that cause bovine respiratory disease (BRD) in beef and non-lactating dairy cattle.. The Thermo Scientific Sensititre System, recognized for including one of the largest and most up-to-date selections of antimicrobials for susceptibility testing, with more than 40 veterinary-specific antimicrobials, is now the first to offer tildipirosin on microbroth dilution susceptibility plates. Tildipirosin is available globally on Sensititre veterinary custom plates, and it will be featured on additional standard plates in the future. A variety of other species-specific, standard diagnostic formats are also available for applications related to avian and poultry, bovine and porcine, companion animals, ...
In vitro antimicrobial activities of ethanol and aqueous “Antibact”, herbal products consisting of a combination of the leaves and branches of four different plants were evaluated against twenty one pathogenic bacteria. Saponins, reducing sugars, phenolics, polyuronides, and triterpenes were the major phyto-constituents of both the aqueous and ethanol “Antibact”. The LD50 analysis revealed the products were safe (LD50>5000 mg/kg bodyweight) for in vivo use. All the isolates (100%) were resistant to at least five of the 12 antibiotics used in the study. In total, the aqueous “Antibact” inhibited the growth of 5 out of the 21 (23.81%) microbes used with an average zone of inhibition of 9.73 ± 0.35 mm. Thirteen (61.90%) out of the 21 microbes used were susceptible to the ethanol “Antibact”, registering an average inhibition zone of 10.80 ± 0.18 mm. In the case of the minimum inhibitory concentration (MIC), the aqueous “Antibact&rdquo
Clinical antimicrobial susceptibility breakpoints are used to predict the clinical outcome of antimicrobial treatment. In contrast, microbiologic breakpoints are used to identify isolates that may be categorized as susceptible when applying clinical breakpoints but harbor resistance mechanisms that result in their reduced susceptibility to the agent being tested. Currently, the National Committee for Clinical Laboratory Standards (NCCLS) guidelines utilize clinical breakpoints to characterize the activity of the fluoroquinolones against Streptococcus pneumoniae. To determine whether levofloxacin breakpoints can identify isolates that harbor recognized resistance mechanisms, we examined 115 S. pneumoniae isolates with a levofloxacin MIC of >2 mg/mL for first-step parC mutations. A total of 48 (59%) of 82 isolates with a levofloxacin MIC of 2 mg/mL, a level considered susceptible by NCCLS criteria, had a first-step mutation in parC. Whether surveillance programs that use levofloxacin data can ...
TY - JOUR. T1 - Yeast-like filamentous fungi. T2 - Molecular identification and in vitro susceptibility study. AU - Esposto, Maria Carmela. AU - Prigitano, Anna. AU - Lo Cascio, Giuliana. AU - Ossi, Cristina. AU - Grancini, Anna. AU - Cavanna, Caterina. AU - Lallitto, Fabiola. AU - Tejada, Milvana. AU - Bandettini, Roberto. AU - Mularoni, Alessandra. AU - Tortorano, Anna Maria. PY - 2019/10/1. Y1 - 2019/10/1. N2 - Yeast-like filamentous fungi, collected in Italy from 1985 to 2018, were submitted to molecular identification and antifungal susceptibility testings. Clinical isolates were identified as Magnusiomyces capitatus (28), M. clavatus (18), and Geotrichum candidum (2). M. clavatus was prevalent among blood isolates (18/24), M. capitatus among isolates from other biological materials. The intrinsic echinocandin resistance was confirmed. Both species had low minimum inhibitory concentrations (MICs) of itraconazole, posaconazole, and voriconazole, while M. clavatus had lower MIC of flucytosine ...
Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans.. A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.. ...
ABSTRACT Ceftaroline (CPT) is a broad-spectrum agent with potent activity against methicillin-resistant Staphylococcus aureus (MRSA). The sequence type 5 (ST5) Chilean-Cordobés clone, associated with CPT nonsusceptibility, is dominant in Chile, a region with high rates of MRSA infections. Here, we assessed the in vitro activity of CPT against a collection of MRSA isolates collected between 1999 and 2018 from nine hospitals (n 320) and community settings (n 41) in Santiago, Chile, and evaluated performance across testing methodologies. We found that our hospitalassociated isolates exhibited higher CPT MIC distributions (MIC50 and MIC90 of 2 mg/liter) than the community isolates (MIC50 and MIC90 of 0.5 mg/liter), a finding that was consistent across time and independent of the culture source. High proportions (64%) of isolates were CPT nonsusceptible despite the absence of CPT use in Chile. Across methodologies, the Etest underestimated the MIC relative to the gold standard broth microdilution ...
Recommended for antimicrobial susceptibility testing of common, rapidly growing bacteria. Buy low price Mueller Hinton Agar Plate in Malakpet, Hyderabad. See My Price. Mueller Hinton Agar with Blood, 15x100mm plate, order by the package of 10, by Hardy Diagnostics (Mueller Hinton Agar with 5% Sheep Blood) For disk diffusion sensitivity testing of Streptococcus spp. Plate Size. Ambient shipping conditions. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. Mueller Hinton Medium Culture Plates Agar HARDY DIAGNOSTICS. ... Mueller Hinton II Agar Plates, Pkg of 20, 150 mm #M0026: Download SDS: SDS Request. 1 Product Result , Match Criteria: Product Name, Description 97580 ; for microbiology, NutriSelect ™ Plus; Millipore pricing. Mueller Hinton Agar Plate offered by Labline Trading Company is available with multiple payment options and easy delivery. Micro BCA Protein Assay Kit, 100-mm Mueller-Hinton agar plates, and Mueller-Hinton liquid broth were ...
Surface plasmon biosensing technology for antimicrobial susceptibility test - References | InTechOpen, Published on: 2011-07-19. Authors: How Foo Chen, Chi-Hung Lin, Chun-Yao Su, et
Antimicrobial resistance is one of today’s major public health challenges. Infections caused by multidrug-resistant bacteria have been responsible for an increasing number of deaths in recent decades. These resistant bacteria are also a concern in the food chain, as bacteria can resist common biocides used in the food industry and reach consumers. As a consequence, the search for alternatives to common antimicrobials by the scientific community has intensified. Substances obtained from nature have shown great potential as new sources of antimicrobial activity. The aim of this study was to evaluate the antimicrobial activity of five bee venoms, also called apitoxins, against two common foodborne pathogens. A total of 50 strains of the Gram-negative pathogen Salmonella enterica and 8 strains of the Gram-positive pathogen Listeria monocytogenes were tested. The results show that the minimum inhibitory concentration (MIC) values were highly influenced by the bacterial genus. The MIC values ranged from
Extended Spectrum Beta-Lactamases (ESBLs) encoding genes (TEM, SHV and OXA) were amplified from multidrug resistance E. coli. The multidrug resistance E. coli isolates from different clinical sources were documented to be plasmid encoded and resistance against β-lactam and cephalosporin. Conventional laboratory analysis showed that seventy percent (70%) of the selected multidrug resistant clinical isolates were ESBLs positive, showing a ≥5 mm increase in zone diameter for either antibiotics compared to its zone when tested alone. The antibiotic susceptibility result showed that 100% of the isolates were resistant to amoxicillin-clavulanic acid, amoxicillin, cefuroxime and ampicillin-sulbactam while 90% of the isolates were resistant to ceftazidine and tetracycline, 80% to ofloxacin, 70% to ceftriazon, nalidixic acid, cefalexin, 60% to ciprofloxacin, 50% to nitrofurantoin, 40% to chloramphenicol and 20% to gentamicine. The multiplex PCR with primers TEM (931bp), SHV (868), OXA-2 (478), aac(3)-IIa (900
Purpose. Rhodomyrtustomentosa is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of R. tomentosa leaf extract against Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia. Methodology. The anti-streptococcal activity of R. tomentosa was determined using broth microdilution assays. Results. The extract demonstrated strong antibacterial activity against the fish pathogens, with minimum inhibitory concentrations (MICs) ranging from 7.8‒62.5 µg ml−1. It was found to possess a dose-dependent bacteriostatic effect on this organism. Scanning electron microscopy revealed irregular and long chains of swollen cells, as well as corkscrew shapes andincomplete separation of cell division of S. agalactiae cells following the treatment at sub-MIC. Moreover, S. agalactiae cells pre-treated with the extract became more
LOWMAN, Warren et al. Comparative MIC evaluation of a generic ceftriaxone by broth microdilution on clinically relevant isolates from an academic hospital complex in South Africa. SAMJ, S. Afr. med. j. [online]. 2012, vol.102, n.2, pp.102-103. ISSN 2078-5135.. We evaluated the in vitro microbiological efficacy of a generic ceftriaxone product against several clinically significant organisms collected from sterile sites. The minimum inhibitory concentration (MIC) of each was determined simultaneously with the reference and the generic ceftriaxone product. Comparative analysis of MICs between the two products for each isolate was performed using both categorical (interpretive) agreement and essential (actual MIC value) agreement. A total of 260 isolates were tested. Overall, there was categorical agreement of 98.9% and essential agreement of 95.8%. The categorical agreement for all isolates (96.7 - 100%) accorded with international standards, as no very major errors were seen and the major error ...
Understanding local susceptibility patterns is important when selecting antimicrobials for initial empirical antibiotic-therapy of bloodstream infections. Because the determination of susceptibility is dependent on the breakpoints used, the aim of the study was to compare the antimicrobial susceptibility results to different classes of antibiotics of 512 strains of Enterobacteriaceae (200 ESβL positive) isolated from bloodstream using CLSI 2013 and current EUCAST 2013 guidelines to evaluate the impact of break-point discrepancies. The results of the study showed that statistically significant discrepancies (p ≤ 0.001) were found for amoxicillin/clavulanic acid, piperacillin alone or with tazobac-tam, imipenem, meropenem, cefepime (only ESβL negative isolates), amikacin and gentamicin using current CLSI or EUCAST interpretive criteria. Further harmonization of CLSI and EUCAST breakpoints is warranted. This study could give useful information to physicians for managing bloodstream