Many neurotransmitters, hormones and sensory stimuli elicit their cellular responses through the targeted activation of receptors coupled to Gq family heterotrimeric G proteins. Nevertheless, we still understand little about the consequences of loss of this signaling activity on brain function. We therefore examined the effects of genetic inactivation of Gnaq on responsiveness in a battery of behavioral tests in order to assess the contribution of Gaq signaling capacity in the brain circuits mediating expression of affective behaviors (anxiety and behavioral despair), spatial working memory and locomotor output (coordination, strength, spontaneous activity and drug-induced responses). First, we replicated and extended findings showing clear motor deficits in Gaq knockout mice as assessed on an accelerating rotarod and the inverted screen test. We then assessed the contribution of the basal ganglia motor loops to these impairments, using open field testing and analysis of drug-induced locomotor
15-Lipoxygenase-2 (ALOX15B), an oxidoreductase in the metabolism of arachidonic acid, is a functional tumor suppressor whose expression is reduced in a variety of human cancers. To determine the roles of ALOX15B in carcinogenesis, we generated transgenic mice with ALOX8, the murine homolog of ALOX15B, knocked out. ALOX8 expression at mRNA level was abolished in homozygous knockout mice and reduced to half of wild type in heterozygous knockout mice. Its observed that homozygous female ALOX8 knockout mice are infertile but male ALOX8 knockout mice are fertile. Increased incidence of tumor as uni or multimass was found in the lung, prostate and in the mesentery region of homozygous and heterozygous ALOX8 knockout mice, when compared with little mate wild type mice. Histological evaluations showed an increase in secondary tumors and inflammation in different tissues like lung and large intestine in mice with ALOX8 knocked out. The transgenic mice could be a good model to study the role of ...
Our results show an important role for the extracellular matrix molecule TN-C in the regulation of neural precursor cell proliferation and migration, as revealed by reduced proliferation of SVZ cells and OP cells in transgenic mice that lack TN-C (Saga et al., 1992). These mice have previously been reported as normal (Saga et al., 1992). Although subsequent studies have revealed changes in behaviour (Fukamauchi et al., 1996; Kiernan et al., 1999) and neurotransmitter levels (Fukamauchi et al., 1996) in the CNS, as well as abnormalities of neuromuscular junction architecture (Cifuentes-Diaz et al., 1998) and repair in the kidney (Nakao et al., 1998), no developmental abnormalities have been described previously. Our results are therefore important in that they demonstrate for the first time a significant role for TN-C in the basic processes of cell growth control during normal development.. The reduction in cell proliferation in the TN-C-deficient animals was revealed by BrdU studies in vivo, ...
There is growing evidence that neuropeptide Y acting through Y1 and Y2 receptors has a prominent role in modulating anxiety- and depression-like behavior in rodents. However, a role of other Y receptors like that of Y4 receptors in this process is poorly understood. We now investigated male Y2, Y4 single and Y2/Y4 double knockout mice in behavioral paradigms for changes in motor activity, anxiety and depression-like behavior. Y4 and Y2 knockout mice revealed an anxiolytic phenotype in the light/dark test, marble-burying test and motor-activity independent in stress-induced hyperthermia, and reduced depression-like behavior in the forced swim and tail suspension tests. In Y2/Y4 double knockout mice, the response in the light/dark test and in the forced swim test was further enhanced compared to Y4 and Y2 knockout mice, respectively. Motor activity was increased in Y2, Y4 and Y2/Y4 knockout mice under changing and stressful conditions, but not altered in a familiar environment. High levels of Y4 ...
Activation of neuropeptide S (NPS) signaling has been found to produce arousal, wakefulness, anxiolytic-like behaviors, and enhanced memory formation. In order to further study physiological functions of the NPS system, we generated NPS precursor knockout mice by homologous recombination in embryonic stem cells. NPS-/- mice were viable, fertile, and anatomically normal, when compared to their wild-type and heterozygous littermates. The total number of NPS neurons-although no longer synthesizing the peptide - was not affected by the knockout, as analyzed in NPS-/- /NPSEGFP double transgenic mice. Analysis of behavioral phenotypes revealed significant deficits in exploratory activity in NPS-/- mice. NPS precursor knockout mice displayed attenuated arousal in the hole board test, visible as reduced total nose pokes and number of holes inspected, that was not confounded by increased repetitive or stereotypic behavior. Importantly, long-term memory was significantly impaired in NPS-/- mice in the ...
We have previously reported the TCR inhibitory molecule CD5 impairs reactivity of tumor-specific PBL-derived T-cell clones against the cognate target and controls their susceptibility to activation-induced cell death (AICD) triggered by tumor cells. In this report, we compared the antitumor T-cell response developed against the B16F10 melanoma engrafted in CD5-deficient and wild-type (wt) C57BL/6 mice. Our results indicate that CD5 knock out mice elicit a delayed tumor growth as compared to wt mice, which is associated with tumor infiltration by more activated tumor-reactive T lymphocytes. Our data also indicate that tumor suppression in CD5-deficient mice is transient and that tumor flare up correlates with increase in AICD of tumor-infiltrating CD8+ T cells. Our data suggest that tumor T lymphocyte infiltration occurs at early stages of cancer development and that tumor-mediated AICD is most likely involved in the induction of T-cell tolerance to malignant cells. ...
M1 Muscarinic Receptor Knockout: support involvement in cognitive processes.. The five Muscarinic Acetylcholine (ACh) receptors are G-protein coupled receptors (M1R-M5R). M1R, M3R and M5R selectively couple to Gq/G11; M2R and M4R selectively couple to Gi/Go. M1R knockout mice are viable and fertile, and have no major morphological abnormalities.. M1 muscarinic receptors are located in higher brain regions of the central nervous system that are involved in cognitive processes. Studies in M1R knockout mice show that M1 receptors may be involved in cortical memory functions that require interactions between the cerebral cortex and hippocampus. Supporting a role for M1 receptor activation in cognition, muscarinic agonist-induced activation of the MAPK pathway, which plays an important role in synaptic plasticity and many cognitive functions, is virtually abolished in primary cortical cultures or CA1 hippocampal pyramidal neurons in M1R knockout mice. ...
TY - JOUR. T1 - Phospholipase β4-knockout mouse exhibits retinal phenotype. AU - Jiang, Huiping. AU - Lyubarsky, A.. AU - Vardi, N.. AU - Pugh Jr, Edward N. AU - Chen, J.. AU - Xu, J.. AU - Simon, M. I.. AU - Wu, Dianqing. PY - 1996/2/15. Y1 - 1996/2/15. N2 - Purpose: Determine if PLC-β4 has a retinal function by making/assessing a mouse knockout. Rationale: PLC-β4 is one of the four PLC-β isoforms that have been cloned and can be activated by the Gα subunits of G-proteins of the Gq class, but not by the Gβγ subunits. PLC-β4 shares a closer homology to the NorpA protein (which mediates phototransduction in Drosopnila) than to the isoforms PLC-β1-β3. Previous immunohistochemical studies have shown that PLC-β4 is expressed in cone photoreceptors, and in bipolar and ganglion cells1. Method: A mouse line was generated in which the PLC-β4 genes are disrupted. Retinal rod function was assessed with single-flash a- and b-wave electroretinography. Anatomical analysis of rod density, rod ...
Its not every day that you get something useful at no charge. But if youre a researcher studying genes that produce secreted and transmembrane proteins, today is your day. A full 472 knockout mouse lines - all extensively characterized (phenotyped) - are now publicly available from the National Institutes of Healths Mutant Mouse Regional Resource Center (MMRRC) at the University of California, Davis. Distribution of the lines is supported by the National Center for Research Resources (NCRR) and the NIH-funded Knockout Mouse Project (KOMP) repository, operated by UC Davis and the Childrens Hospital Oakland Research Institute in Oakland, Calif. Knockout mouse lines have served as valuable models to study a range of human conditions, from obesity and heart disease to diabetes and substance abuse. In knockout laboratory mice, researchers have inactivated, or "knocked out", a gene with an artificial piece of DNA. This helps scientists infer what a gene normally does by understanding what goes ...
Author: Ebinger, M. et al.; Genre: Journal Article; Published in Print: 2005-09; Title: Is testosterone a substrate of P-glycoprotein in abcb1ab knock out mice?
TY - JOUR. T1 - Mamu-A*01/Kb transgenic and MHC Class I knockout mice as a tool for HIV vaccine development. AU - Li, Jinliang. AU - Srivastava, Tumul. AU - Rawal, Ravindra. AU - Manuel, Edwin. AU - Isbell, Donna. AU - Tsark, Walter. AU - La Rosa, Corinna. AU - Wang, Zhongde. AU - Li, Zhongqi. AU - Barry, Peter A. AU - Hagen, Katharine D.. AU - Longmate, Jeffrey. AU - Diamond, Don J.. PY - 2009/4/25. Y1 - 2009/4/25. N2 - We have developed a murine model expressing the rhesus macaque (RM) Mamu-A*01 MHC allele to characterize immune responses and vaccines based on antigens of importance to human disease processes. Towards that goal, transgenic (Tg) mice expressing chimeric RM (α1 and α2 Mamu-A*01 domains) and murine (α3, transmembrane, and cytoplasmic H-2Kb domains) MHC Class I molecules were derived by transgenesis of the H-2KbDb double MHC Class I knockout strain. After immunization of Mamu-A*01/Kb Tg mice with rVV-SIVGag-Pol, the mice generated CD8+ T-cell IFN-γ responses to several known ...
Nmu (Neuromedin U) and Nmus (Neuromedin S) gene double knock-out mice. Nmu KO: Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms KO: Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nms gene knockout mice (RBRC04550 ...
In humans, there are over 400 genetic syndromes that include a hearing loss component, but most of the genes underlying hearing loss syndromes are currently unknown. The knockout mice tested so far in this study represented only about 15 percent of mouse genes, so the researchers estimate that if the entire genome is searched there will be at least 450 genes required for hearing function.. Professor Steve Brown, senior author on the paper and director of British Medical Research Councils Harwell laboratory, said: "Importantly, the large number of hearing loss genes identified in this study demonstrates that there are many more genes involved in deafness in mouse and human genomes than we had previously realised.. "Our findings identify 52 genes that have previously not been recognised as being critical for hearing. These increase our knowledge of the many genes and molecular mechanisms required for hearing, and also provide a shortlist of new genes to investigate to discover the genetic basis ...
If one parent is a wild-type mouse and the other is a homozygous knockout mouse, their offspring will be heterozygous at the knockout gene. The mouse will likely produce the protein from the wild-type copy of the gene, but depending on how the gene is regulated it is likely that expression of the protein will be below wild-type levels ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Approach and Results-We bred the macrophage-specific L13a knockout mice L13a Flox+/+ Cre+/+ onto apolipoprotein E-deficient background and generated the experimental double knockout mice L13a Flox+/+ Cre+/+ apolipoprotein E deficient (apoE−/−). L13a Flox+/+ Cre−/− mice on apolipoprotein E-deficient background were used as controls. Control and knockout mice were subjected to high-fat diet for 10 weeks. Evaluation of aortic sinus sections and entire aorta by en face showed significantly higher atherosclerosis in the knockout mice. Severity of atherosclerosis in knockout mice was accompanied by thinning of the smooth muscle cell layer in the media, larger macrophage area in the intimal plaque region and higher plasma levels of inflammatory cytokines. In addition, macrophages isolated from knockout mice had higher polyribosomal abundance of several target mRNAs, thus showing defect in translation control.. ...
Recent Publications. Hill S, Deepa SS, Sataranatarajan K, Premkumar P, Pulliam D, Liu Y, Soto VY, Fischer KE, Van Remmen H. Sco2 deficient mice develop increased adiposity and insulin resistance. Mol Cell Endocrinol. 2017 Nov 5;455:103-14. PMCID:PMC5592144. Sakellariou G, McDonagh B, Porter H, Giakoumaki I, Earl K, Nye G, Vasilaki A, Brooks S, Richardson A, Van Remmen H, McArdle A, Jackson MJ. Comparison of whole body SOD1 knockout with muscle specific SOD1 knockout mice reveals a role for nerve redox signaling in regulation of degenerative pathways in skeletal muscle. Antioxid Redox Signal. 2017 Oct 25. PMID: 29065712. Zhang N, Valentine JM, Zhou Y, Li ME, Zhang Y, Bhattacharya A, Walsh ME, Fischer KE, Austad SN, Osmulski P, Gaczynska M, Shoelson SE, Van Remmen H, Chen HI, Chen Y, Liang H, Musi N. Sustained NFκB inhibition improves insulin sensitivity but is detrimental to muscle health. Aging Cell. 2017 Aug;16(4):847-58. PMCID:PMC5506420. Selected Publications. Masser DR, Clark NW, Van Remmen ...
The ability to genetically modify mice is a powerful tool used in basic and applied research with many applications for the study of gene function and human disease. A current world-wide initiative is generating a knockout mouse strain for every protein coding gene using embryonic stem (ES) cells. Mouse strains and ES cells from these initiatives are made available to the worldwide research community via public repositories.. Once phenotyped, mouse models provide invaluable insights into human gene function with wide-ranging clinical implications, including better understanding of diseases and discovering gene targets for therapeutic agents.. The Embryonic Stem (ES) cell to Mouse (ES2M) service is a core APN facility which provides ready access to the global initiative to discover functional insight for every gene by generating and systematically phenotyping 20,000+ knockout mouse strains. The ES2M service enables Australian researchers to choose the genetically modified ES cell line(s) or mice ...
Sakellariou, GK,McDonagh, B,Porter, H,Giakoumaki, II,Earl, KE,Nye, GA,Vasilaki, A,Brooks, SV,Richardson, A,Van Remmen, H,McArdle, A,Jackson, MJ (2018) Comparison of Whole Body SOD1 Knockout with Muscle-Specific SOD1 Knockout Mice Reveals a Role for Nerve Redox Signaling in Regulation of Degenerative Pathways in Skeletal Muscle. Antioxidants & Redox Signaling, 28 :275-295 [DOI] [Details] ...
PTK787 2HCl and -7 (Lakhani et al. 2006 offers contributed to your knowledge of the physiological tasks of the caspases significantly. Oddly enough C57BL/6 mice deficient for both caspase-3 and -7 perish shortly after delivery while mice missing only caspase-3 or -7 have a normal life span and display a limited apoptotic phenotype in this genetic background (Lakhani et al. 2006 Leonard et al. 2002 This points to the functional redundancy between caspase-3 and -7 during embryogenesis. However several observations suggest that this overlap is not complete and that caspase-3 and -7 also fulfill non-redundant roles in apoptosis. For instance eye lenses of caspase-7 knockout mice are grossly normal whereas those of caspase-3 deficient mice display marked cataracts at the anterior lens pole (Zandy et al. 2005 Further support for this notion stems from biochemical studies demonstrating that caspase-3 and -7 exhibit differential activities toward multiple protein substrates with caspase-7 being more ...
J. K. White, Gerdin, A. - K. , Karp, N. A. , Ryder, E. , Buljan, M. , Bussell, J. N. , Salisbury, J. , Clare, S. , Ingham, N. J. , Podrini, C. , Houghton, R. , Estabel, J. , Bottomley, J. R. , Melvin, D. G. , Sunter, D. , Adams, N. C. , Tannahill, D. , Logan, D. W. , Macarthur, D. G. , Flint, J. , Mahajan, V. B. , Tsang, S. H. , Smyth, I. , Watt, F. M. , Skarnes, W. C. , Dougan, G. , Adams, D. J. , Ramirez-Solis, R. , Bradley, A. , and Steel, K. P. , "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.", Cell, vol. 154, no. 2, pp. 452-64, 2013. ...
In previous studies using a Cdc42GAP knockout, Cdc42 gain-of-activity mouse model, we have shown that constitutively increased Cdc42-GTP species can lead to defective hematopoietic stem/progenitor survival, adhesion, and actin cytoskeleton that may contribute to an engraftment defect in hematopoietic stem/progenitors.20 Cdc42GAP−/− mice also displayed mild anemia and deficiencies in erythroid progenitors. While this animal model provides valuable information for a possible involvement of Cdc42 activity in hematopoiesis, there are inherent weaknesses of the gain-of-activity approach that complicate interpretation of the data as they relate to the physiologic role of Cdc42. For example, this animal model cannot reveal the requirement of Cdc42 in hematopoiesis nor rebut a valid criticism that Cdc42GAP knockout may also bring about Cdc42-independent effects.. To understand the physiological role of Cdc42, we have recently generated a Cdc42-conditional knockout mouse model that uses the loxP/Cre ...
Caspr3-Deficient Mice Exhibit Low Motor Learning during the Early Phase of the Accelerated Rotarod Task. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Estrogen Receptor-α Knock-Out Mouse Model is an eagle-i resource of type Mus musculus at Charles Drew University of Medicine and Science.
We offer a unique portfolio of transporter humanized and knockout mouse models which can be used for different in vivo applications.
Hus1 inactivation results in abnormal accumulation of γH2AX on autosomes, an extended sex body domain, inclusion of autosomes within the sex body, and X-autoso
The goal of this RFA is to produce the largest number of null mutants possible for the dollars available. NIH recognizes that the dollar figure given in the RFA will demand applicants to propose very aggressive programs that minimize cost while still providing a quality product. We aim to maximize the use of NIH dollars and intend to fund a balanced program that may include a set of applications performing different types of knockout mutations in order to cover as much of the genome as possible with the dollars available. Applicants should propose the best approach they can for accomplishing the goals of maximizing the number of null mutations, cost reduction and quality. The actual balance of the program and costs per knockout will be determined as a result of peer review, Council discussion and staff negotiation with the applicants before funding ...
FUNCTION: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015 ...
Albinism is the best-known of a group of rare genetic disorders that can affect both eyes and skin. Some genes have been identified that are linked to these conditions, but many remain mysterious. Now a team led by UC Davis researchers has identified dozens of these genetic mutations in a screen of gene-targeted "knockout" mice. The authors hope the work, published Aug. 1 in Scientific Reports, will be a resource for clinicians specializing in genetic disorders.. "This mouse data may be of interest to clinicians, especially for patients with no known genetic cause for their condition," said Ala Moshiri, associate professor of ophthalmology in the UC Davis School of Medicine and corresponding author on the paper.. Skin, eyes and nerve tissue are linked because they all develop from the same early embryonic tissue. Another group of rare eye and skin disorders distinct from albinism, called phakomatoses, are also caused by genetic alterations inherited from parents or that occur by accident early ...
Genetic exploration of novel behavioral phenotypes in interleukin-7 and interleukin-18 receptor knockout mice. by Amy F. Eisener-Dorman full download exe or rar online without authorization for free.
Fast delivery of LRRC8B knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. Includes matched wildtype control.
Fast delivery of DNMT3B knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. Includes matched wildtype control.
Safe Knockout to generate both conventional and tissue-specific Knockout mice from a single project, and substantially save time and cost.
Accelerate your research with custom-engineered knockout mouse models. Design your own conditional and constitutive knockout mice or use existing strains.
MyD88-dependent signaling in myo-/fibroblasts (MFs) is involved in epithelial barrier restoration and tolerance. However, the role of MyD88-dependent signaling by MFs in the regulation of inflammatory responses by macrophages in the colonic mucosa is poorly understood. Because colonic MFs respond to MyD88 activation with production of molecules involved in the regulation of macrophages (PGE2, PD-L1, etc.), we hypothesize that MF mediated MyD88 signaling regulates inflammatory responses from macrophages in the colon. Tamoxifen inducible Col1α2Cre Myd88 floxed mice (fibroblast and myofibroblast-specific MyD88 deletion) and α-SMACre MyD88 floxed mice (myo-fibroblast and smooth muscle cell-specific MyD88 deletion) on the same genetic background (C57BL/6) were used in this study. We observed that deletion of MyD88 within MFs resulted in the inflammatory changes and infiltration of lymphocytes within colonic mucosa and moderately aggravated DSS induced acute colitis. Activation of the lymphocyte ...
What is the advantage of knockout over gene therapy in mice model in order to st - posted in General Lab Techniques: Hi, Am going through the literature of TFPI-2 protein, where some people have studied its role in apoE knockout mice by replacing TFPI-2 gene by gene therapy..thereby upregulating its expression, and later also downregulating it via respective vector constructs. Of approximately, 10 years of study of this protein no one introduced any knockout mice...
PANcreatic-DERived Factor (PANDER), or FAM3B, is a 235-amino acid protein strongly expressed within and secreted from the endocrine pancreas. Research surrounding PANDER has revealed a large role for the protein in maintaining glucose homeostasis, as evidenced by several Ad-PANDER overexpressing murine models, our labs pancreas-specific PANDER transgenic overexpressor, and most recently our mixed genetic C57/129J PANDER knockout (PANKO) mouse. However, PANDERs overall role in glycemic regulation and glucose homeostasis has yet to be studied in a purebred C57BL/6J PANDER knockout model. Here we present the first phenotypic characterization of our global PANDER knockout mouse on a C57BL/6J background (PANKO-C57) where we examined metabolics through glucose/insulin tolerance testing, fasting glycemia, and body weights, the concentrations of hormonal analytes along with lipids and corticosterones, and full elucidation of hepatic insulin signaling through the insulin signaling cascade. Overall, the PANKO
Knockout mouse: Knockout mouse, genetically engineered laboratory mouse (Mus musculus) in which a specific gene has been inactivated, or
Animals. Construction of K19-C2mE transgenic mice was described previously ( 3). Mice homozygous null for TNF-α (Tnf), interleukin-1 (IL-1) receptor α chain (Il1r1), and Rag2 were purchased from Jackson Laboratory, Bar Harbor, ME (Tnf and Il1r1) and from Taconic, Germantown, NY (Rag2). The K19-C2mE mice were crossed with respective knockout mouse strains to generate Tnf (−/−) K19-C2mE, Il1r1 (−/−) K19-C2mE, and Rag2 (−/−) K19-C2mE mice. Littermate simple K19-C2mE transgenic mice were used as controls. Compound mutants and control mice were examined histologically at 20 weeks of age (n = 10 for each genotype). For treatment with meloxicam (Daiichi Pharmaceutical, Tokyo, Japan), K19-C2mE mice were dosed with 10 mg/kg/d of the compound by oral administration for 3 weeks in mice that were 78 to 80 weeks of age. All animal experiments were carried out according to the protocol approved by the Ethical Committee at Kyoto University.. Histopathology and immunohistochemistry. Tissues were ...
Ready-to-use Knockout mouse models for target discovery and confirmation, in vivo compound specificity, MOA and clinical studies.
Publication: McCarthy DM, Gioioso V, Zhang X, Sharma N, Bhide PG (2012b) Neurogenesis and neuronal migration in the forebrain of the TorsinA knockout mouse embryo. Developmental neuroscience 34:366-378.. Additional Information. ...
The data presented here indicate that inhibition of β- or γ-secretase in neurons can compromise cell viability. In three different neuronal phenotypes, the pharmacological knock-down of amyloidogenic secretase activity resulted in cell death.. Is the toxicity of these compounds attributable to inhibition of Aβ production? There are several lines of evidence that suggest this to be the case. First, these compounds have been shown to produce a profound inhibition of Aβ production at the concentrations used in this study. Greater than 99% inhibition of de novo Aβ production would be expected at γ-IV concentrations used here (Beher et al., 2001), and our own immunocytochemical data would appear to confirm this. Second, the production of Aβ has been prevented in two ways via the pharmacological inhibition of two distinct and separate enzymes. Importantly, all compounds used were structurally different, suggesting that toxicity was not the nonspecific result of a particular chemical moiety. ...
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Transcriptome data from the gene knockout experiment in mouse is widely used to investigate functions of genes and relationship to phenotypes. When a gene is knocked out, it is important to identify which genes are affected by the knockout gene. Existing methods, including differentially expressed gene (DEG) methods, can be used for the analysis. However, existing methods require cutoff values to select candidate genes, which can produce either too many false positives or false negatives. This hurdle can be addressed either by improving the accuracy of gene selection or by providing a method to rank candidate genes effectively, or both. Prioritization of candidate genes should consider the goals or context of the knockout experiment. As of now, there are no tools designed for both selecting and prioritizing genes from the mouse knockout data. Hence, the necessity of a new tool arises. In this study, we present CLIP-GENE, a web service that selects gene markers by utilizing differentially expressed genes
Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
Faced with continuing constraints, companies need more flexible options to find capacity in a dynamic transportation environment.
Faced with continuing constraints, companies need more flexible options to find capacity in a dynamic transportation environment.
Attached ako sa bagay-bagay. Nawala yung eraser ko dati. Faber-Castell pa naman yun. ilang araw akong balisa, hindi ko matanggap na nawala na sya, iniwan na ako! Mas malupit dati, noong Grade 3 ako. Naiwan ko yung pencil case ko na bagong bili. yung maraming pindutan, pindutan para lumabas yung sharpener, eraser at kung ano-ano pa. usong-uso noon yun, kaya naman tuwang-tuwa ako ng binili ako ng nanay ko. Dumayo kami ng Math contest, tapos yun nga, naiwan ko yung pencil case, naalala ko na lang, nasa byahe na kami pauwi.. tapos nung naalala ko, bigla na lang ako napaiyak, hindi pala iyak, napahagulgol ako sa lungkot. para tumugil ako sa paghagulgol, ibibili na lang daw ako ulit.. eh sa hirap ng buhay, hindi na ako nabili ulet. Ganun ako ka-senti ...
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The SMURF2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The LGALS3 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Background ELF2 (E74-like aspect 2) also known as NERF (brand-new (E-twenty-six) family members of transcription elements, characterised by the existence of an evolutionarily conserved 85 amino acidity (aa) DNA-binding domains, utilises a range of elements to govern focus on specificity. redundant guests might occur in sites throughout the genome [7]. Simple distinctions in sites, tissue-specific reflection of elements and their co-factors, and differential signalling replies might all lead to their distinctive features, but makes determining accurate goals both challenging and complicated [8, 9]. Particular protein are known to KRN 633 play essential tasks in haemopoietic advancement via transcriptional legislation. Knockout mouse versions possess helped unravel the practical importance of protein in haemopoiesis. Reduction of PU.1 (SPI1) has a profound impact on haemopoietic development by affecting myeloid and B cell development [10, 11]. Additional gene knockout mouse versions with problems ...
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Limited Competition: Knockout Mouse Production and Phenotyping Project (UM1) RFA-RM-15-017. Roadmap
Background ELF2 (E74-like aspect 2) also known as NERF (brand-new (E-twenty-six) family members of transcription elements, characterised by the existence of an evolutionarily conserved 85 amino acidity (aa) DNA-binding domains, utilises a range of elements to govern focus on specificity. redundant guests might occur in sites throughout the genome [7]. Simple distinctions in sites, tissue-specific reflection of elements and their co-factors, and differential signalling replies might all lead to their distinctive features, but makes determining accurate goals both challenging and complicated [8, 9]. Particular protein are known to KRN 633 play essential tasks in haemopoietic advancement via transcriptional legislation. Knockout mouse versions possess helped unravel the practical importance of protein in haemopoiesis. Reduction of PU.1 (SPI1) has a profound impact on haemopoietic development by affecting myeloid and B cell development [10, 11]. Additional gene knockout mouse versions with problems ...
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These |i| Xrcc5|/i| knock-out mice exhibit growth deficiency including decreased size of spleen, lymph nodes and thymus with curtailed T cell and B cell development.
J:60742 Rosenfeld ME, Prichard L, Shiojiri N, Fausto N, Prevention of hepatic apoptosis and embryonic lethality in RelA/TNFR-1 double knockout mice. Am J Pathol. 2000 Mar;156(3):997-1007 ...
Cell Biologics provides custom services to our customers upon request. On request, we will isolate cells of any description from specific murine organs or tissues designated by our customers. For example, we are well versed in isolating endothelial cells from wild-type and knock-out murine strains from any desired tissue. Customer may ship to Cell Biologics either the mouse or the already excised murine tissue, and we will isolate the desired cell type and return a vial to Customer containing the prepared cells ready to use. If you have any special needs in isolation of endothelial cells, macrophages, bone marrow derived cells or other cell types from wild type or knockout or other genetically modified mice, please contact us for this special service. Let us know what you need and we will accommodate you. For details regarding these special services contact us at [email protected] or submit Custom Inquiry Form on our website http://www.cellbiologics.com. We will contact you as soon as ...
Cell Biologics provides custom services to our customers upon request. On request, we will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated by our customers. For example, we are well versed in isolating endothelial cells and epithelial cells from animals (wild-type and knock-out murine strains, rat strains) from any desired tissue. Customer may ship to Cell Biologics either the already excised tissues or live mice or rats, and we will isolate the desired cell type and return a vial to Customer containing the prepared cells ready to use. If you have any special needs in isolation of endothelial cells, epithelial cells, macrophages, bone marrow derived cells or other cell types from animal or human tissues, please contact us for this special service. Let us know what you need and we will accommodate you. ...
Hmm, vidím že birdzáci sú tu nejakí bars výberaví.. (?) (hehe) ...ja mám vysokú školu a ako bolo by to krásne a pekné mať plat ako chcela väčšina 1200€ v hrubom, ale kto mi tu v tejto oblasti takú dá, keď by som musel robiť vrcholovú pozíciu a som študent bez praxe, čo ma aj štve, ale tak aj vizitka školy je to.. :-/ ...darmo, robil som zatiaľ v robote, kde to bolo v hrubom za 750€ a bol som rad, že som rád a každý vysokoškolák by sa na takú robotu najradšej vykašľal.. (sorry ...
We offer a unique portfolio of transporter humanized and knockout mouse models which can be used for different in vivo applications.
Based on automated MP annotations supported by experiments on knockout mouse models. Click on icons to go to all Slc5a5 data for that phenotype. ...
Based on automated MP annotations supported by experiments on knockout mouse models. Click on icons to go to all Dnase1 data for that phenotype. ...
In a world characterized by rapid change and increasing levels of complexity and uncertainty, asset management companies see new threats of disruption on the horizon.
Trouvez tous les livres de Giamila Fantuzzi (Editor) - Cytokine Knockouts. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9781617374159
Trenorol je trenbolon podobný vzorec steroid, ktorý pomáha človeku zvýšiť na budovanie svalov bez vzniklo akékoľvek riziko pre zdravie. Výrobok by mal byť sprevádzaný niekoľkými cvičenia a iných fyzických cvičení pre lepšie výsledky, ktoré sa majú uskutočniť počas krátkej doby. Pri používaní tohto výrobku, tam je takmer sto percent zárukou bezpečnosti používania a účinnosti. Je vyrábaný, vlastnil a distribuovaný pomocou Spojených štátov amerických založená spoločnosť známu ako bláznivý Hrčka, ktorý vyniká vo výrobe športových a fitnes doplnkov, ktoré sú lákavé a zdravé na použitie.. ...
ahoj kočky, v stredu idem na kyret. Doktorka my to aja vysvetlila aj všetko, len som bola v takom stave, že som ju aninevnímala, poradte mi, čo si mám vziať so sebou, ako dlho to bude trvať a na čo si mám dať pozor? za všetky odp. dakujem
In Sidering Knockout, you goal is to knock all your opponents out cold and get the championship belt! Try to use combinations and dodge the opponents punches. A good tip would be to find the best key combination and use it all the time. Try not to get hit by spamming punches. Note: we are not responsible for any damage you may do to your keyboard! Show them whos boss in the ring. ...
Important facts & side effect information that you must know. See what the science says in our Instant Knockout Review. Read now.
In truth, Tottenham never really looked like taking all three points and this defeat means they face a battle to reach the knockout stages -with their next home game against PAOK Salonika on 30 November likely to prove decisive. ...
We take a look back at 2017 to determine the best of the best in MMA. What was the top fight? Who had the top knockout? Who performed the best submission?
Sastojci: 200g pecene soje, 130ml vode + jos ako je potrebno, 1/4 kasicice soli (samo ako koristite neslanu soju), 2 kasike kokosovog ulja ili ulje od uljane repice ili maslinovog ulja, 1 kasika agava sirupa. Priprema: 1. U ciniju staviti soju, preliti vodom i ostaviti da nabubri. 2. U blender staviti soju (i vodu ako je…
Ywhae knockout mouse is useful for pathophysiological analyses of neuropsychiatric disorders caused by defects during neurodevelopment ...
Obnoviteľné zdroje energie (OZE) sú také zdroje, ktoré sa prirodzene obnovujú v priebehu ich využívania. Ide o energetické toky, ktoré sa prirodzene vyskytujú v blízkosti zemského povrchu, zásoby, ktoré sa obnovujú aspoň tak rýchlo, ako sú spotrebuvávané. V ich čerpaní možno hypoteticky pokračovať ďalšie miliardy rokov - v podstate kým bude svietiť Slnko. Konkrétne ide o slnečné žiarenie a z toho odvodenú veternú energiu a vodnú energiu, ďalej o energiu prílivu, geotermálnu energiu, biomasu atď. Technológie obnoviteľných zdrojov energie slnečnú energiu, a veternú energiu. V roku 2006, približne 18 % celkovej svetovej spotreby energie pochádzalo z obnoviteľných zdrojov energie; 13 % z tradičnej biomasy spôsobmi akým je spaľovanie dreva. Vodná energia bola ďalším najväčším obnoviteľným zdrojom, poskytujúca 3 %, nasledovaná horúcou vodou na vykurovanie ktorá prispela 1,3 %. Moderné technológie, ako geotermálna, veterná, ...
Plody su velmi vyzivne a bohate na vitamin C (je dolezitym antioxidantom, ktory chrani telesne tkaniva a ostatne vitaminy. Je potrebny pre regulaciu normalneho telesneho rastu, hojenie ran a reakcii na stres a stimuluje tvorbu bielych krviniek). Obsah vitaminu C je nizsi ako v guave, ale vyssi ako v citrusoch a jablkach. Plody su dalej bohate na ...
Občianske združenie IPASK (Iniciatíva pôrodných asistentiek Slovenska) je dobrovoľnou spoločenskou mimovládnou organizáciou, ktorá vznikla z potreby zviditeľniť a posilniť profesijný status, uplatnenie kompetencií a všetkých rolí pôrodnej asistentky (PA) v praxi ako zdravotníckej profesionálky, ako rovnocenného poskytovateľa zdravotnej starostlivosti s autonómnym a regulovaným povolaním v súlade s právnymi normami SR, direktívami EÚ, odporúčaniami Svetovej zdravotníckej organizácie (WHO) a ostatných medzinárodných zdravotníckych inštitúcií (FIGO, ICM, EMA…)
OriGene offers the most comprehensive source for human proteins and lysates, including purified proteins and corresponding MS standard, over expression lysates, cancer cell lysates and knockout cell lysates.
Narodne novine d.d se obvezuju poštivati anonimnost i privatnost korisnika ovih internetskih stranica. O posjetiteljima se neće prikupljati nikakvi osobni podaci osim u slučajevima ako ih posjetitelj dobrovoljno dostavi Narodnim novinama d.d. U slučajevima kad je poznat indentitet posjetitelja/pošiljatelja, njegovi će se podaci koristiti samo u svrhu zbog koje ih je pošiljatelj poslao. Narodne novine d.d. takve podatke mogu koristiti i za što bolji uvid i razumijevanja pojedinačnih potreba i zahtjeva korisnika kao i razvijanja mogućnosti što kvalitetnijega pružanja svojih usluga korisnicima. Narodne novine d.d. se obvezuju da navedene podatke neće učiniti dostupnim bilo kojoj trećoj osobi odnosno strani bez izričitoga pristanka korisnika ...
Ako už názov napovedá, značka Royal Canin sľubuje vášmu psíkovi a mačičke kráľovské pochutnávanie. U nás kompletná ponuka skladom.
Approach and Results-SR-BI/LDLR double knockout and control LDLR knockout mice were fed atherogenic diets containing different amounts of fat, cholesterol, and sodium cholate. Double knockout mice fed atherogenic diets high in cholesterol exhibited significantly reduced survival compared with LDLR knockout mice fed the same diets. In addition to increased diet-accelerated aortic sinus atherosclerosis, we observed significant diet-induced CA atherosclerosis in double knockout mice and diet-dependent accumulation of platelets in CA atherosclerotic plaques. This was accompanied by substantial myocardial fibrosis in double knockout mice fed high cholesterol diets. Atherogenic diet fed double knockout mice also exhibited higher circulating cytokine levels, monocytosis with increased proportions of Ly6Chi and Ly6Cint monocytes, and higher adhesion molecule expression in CA endothelial cells compared with control LDLR knockout mice.. ...
Background and aims: Perilipin1 (PLIN1), a lipid droplet-associated protein, plays an important role in the regulation of lipolysis and lipid storage in adipocytes. PLIN1 has recently been reported to be expressed in macrophages within atheroma plaques, suggesting PLIN1 may play a role in the accumulation of lipids at the arterial wall and in the development of atherosclerosis. To clarify the role of PLIN1 in the pathophysiology of atherosclerosis, we assessed the progression of atherosclerosis in PLIN1 transgenic mice (Plin1Tg). Methods: Plin1Tg were crossed with apolipoprotein E knockout mice (ApoeKO). C57BL/6J mice, ApoeKO and Plin1Tg/ApoeKO received a normal chow diet for 20 weeks. Body weight, gonadal fat mass and plasma lipid concentrations were measured. Aortas were collected for quantification of atheroma lesions and histological analysis by Oil Red O staining. Results: Body weight, gonadal adipose mass and plasma triglyceride concentrations were not significantly different among the ...
Mice with homologous disruption of the interferon gamma (IFN-gamma) gene on the C57BL/6 background were infected with Leishmania major and the immune response assessed. In contrast to wild-type or heterozygous knockout mice, deficient animals were unable to restrict growth of the parasite and suffered lethal infection over 6-8 wk. Although wild-type and heterozygous littermates developed CD4+ cells that contained transcripts for IFN-gamma and lymphotoxin, typical of T helper type 1 (Th1) cells, the knockout mice developed CD4+ cells that contained transcripts for interleukin 4 (IL-4), IL-5, and IL-13, typical of Th2 cells. ELISPOT assays confirmed the reciprocal patterns of IFN-gamma or IL-4 production by T cells in similar frequencies in the respective groups of mice, and antibody analysis confirmed the presence of Th2-mediated isotype switching in the knockout mice. These data suggest that CD4+ T cells that normally respond to antigens by differentiation to Th1 cells default to the Th2 pathway ...
TY - JOUR. T1 - Structural and functional abnormalities in the spleen of an mFtz-F1 gene-disrupted mouse. AU - Morohashi, Ken ichirou. AU - Tsuboi-Asai, Hisae. AU - Matsushita, Sumie. AU - Suda, Masahiro. AU - Nakashima, Manabu. AU - Sasano, Hironobu. AU - Hataba, Yoshiaki. AU - Li, Chun Li. AU - Fukata, Junichi. AU - Irie, Junji. AU - Watanabe, Takeshi. AU - Nagura, Hiroshi. AU - Li, En. PY - 1999/3/1. Y1 - 1999/3/1. N2 - The spleen has two main functions. The first is to provide a proper microenvironment to lymphoid and myeloid cells, whereas the second involves clearance of abnormal erythrocytes. Ad4BP/SF-1, a product of the mammalian FTZ-F1 gene (mFTZ-F1), was originally identified as a steroidogenic, tissue- specific transcription factor. Immunohistochemical examination of the mammalian spleens confirmed the expression of Ad4BP/SF-1 in endothelial cells of the splenic venous sinuses and pulp vein. In mFtz-F1 gene-disrupted (KO) mice, several structural abnormalities were detected in the ...
TY - JOUR. T1 - b1-Adrenoceptors compensate for b3-adrenoceptors in ileum from b3-adrenoceptor knock-out mice. AU - Hutchinson, Dana Sabine. AU - Evans, Bronwyn A. AU - Summers, Roger J. PY - 2001. Y1 - 2001. M3 - Article. VL - 132. SP - 433. EP - 442. JO - British Journal of Pharmacology. JF - British Journal of Pharmacology. SN - 1476-5381. IS - 2. ER - ...
Aim: To determine whether high-mobility group box 1 (HMGB1) and Toll like receptor 4 (TLR4) drive the inflammatory cascade that promotes intimal hyperplasia (IH) following acute vascular injury. Methods and Results: Carotid artery wire injury in C57BL/6 mice induced a significant increase in intima to media (I/M) ratio at four weeks. Global deletion of HMGB1 using an inducible knockout mouse strain caused prevention of IH. IH was decreased by over 50% in WT mice treated with HMGB1 neutralizing antibody. Of knockout mouse strains deficient in putative receptors for HMGB1, TLR4-/- mice showed the greatest inhibition of IH. Both anti-HMGB1 treated mice and TLR4-/- mice exhibited a marked decrease in monocytic recruitment. Mice with selective depletion of TLR4 from macrophage exhibited a similar level of inhibition of IH to that seen in the global TLR4-/-. In vitro, dithiol HMGB1 dose-dependently promoted SMC migration and MCP-1/CCR2 expression, which was abolished by TLR4 inhibitory peptide. ...
Is it possible to get the striatum astrocyte-specific Cre recombinase-mediated knockout of XX gene knockout mice? Any one understand how to make the knockout locate in striatum or any other good ideas to knockout one gene in one special cell from one..
TY - JOUR. T1 - PKCδ Knockout Mice Are Protected from Dextromethorphan-Induced Serotonergic Behaviors in Mice. T2 - Involvements of Downregulation of 5-HT1A Receptor and Upregulation of Nrf2-Dependent GSH Synthesis. AU - Tran, Hai Quyen. AU - Lee, Youngho. AU - Shin, Eun Joo. AU - Jang, Choon Gon. AU - Jeong, Ji Hoon. AU - Mouri, Akihiro. AU - Saito, Kuniaki. AU - Nabeshima, Toshitaka. AU - Kim, Hyoung Chun. PY - 2018/10/1. Y1 - 2018/10/1. N2 - We investigated whether a specific serotonin (5-HT) receptor-mediated mechanism was involved in dextromethorphan (DM)-induced serotonergic behaviors. We firstly observed that the activation of 5-HT1A receptor, but not 5-HT2A receptor, contributed to DM-induced serotonergic behaviors in mice. We aimed to determine whether the upregulation of 5-HT1A receptor induced by DM facilitates the specific induction of certain PKC isoform, because previous reports suggested that 5-HT1A receptor activates protein kinase C (PKC). A high dose of DM (80 mg/kg, i.p.) ...
Previous studies in male AT2R knockout mice suggest that this receptor contributes to BP control.6 Compared with their wild-type littermates, male AT2R knockout mice exhibit slightly higher systolic BP and induce a more rapid and pronounced increase in BP in secondary models of hypertension, such as volume-expansion-induced hypertension produced by deoxycorticosterone acetate-salt. Controversy exists, however, over the role of the AT2R in modulating the effectiveness of AT1R blockade at lowering BP in male rat models of renin-dependent (eg, 2-kidney, 1-clip) and -independent (eg, the spontaneously hypertensive rat) hypertension, because Ang II hypersensitivity in the absence of the AT2R may simply reflect AT1R upregulation. It is indeed a pity that female AT2R knockouts were not investigated in these studies, because one might predict that they would exhibit an even greater difference in BP and a greater degree of Ang II hypersensitivity compared with their wild-type littermates than the males, ...
Authors: Melissa M Thomas, David C Wang, Donna M DSouza, Matthew P Krause, Andrew S Layne, David S Criswell, Hayley M ONeill, Michael K Connor, Judy E Anderson, Bruce E Kemp, Gregory R Steinberg, Thomas J Hawke
The use of animal models led to great progresses in understanding the role of the endosomal adaptor Lamtor2 in endosomal/lysosomal trafficking. In a first approach LAMTOR2 knockout mice were generated, but severe defects during embryogenesis resulted in no viable offspring (Teis et al., 2006). During this time, 4 patients, all siblings, suffering from a primary immunodeficiency syndrome, due to a hypomorph LAMTOR2 allele, were identified and demanded for further investigations on the function of LAMTOR2 for the immune system (Bohn et al., 2007). Therefore, we have established conditional knockout mouse models to investigate the role of LAMTOR2 for the immune system. A special interest was put on antigen presenting cells including macrophages and dendritic cells (DCs). The correct uptake and processing of pathogens and antigen presentation in the context of the immune response is strictly regulated by the endosomal/lysosomal system. Using a mouse model where Lamtor2 was specifically depleted in ...
Experimental and clinical studies suggest that the vascular endothelium may play an important role in modulating the progression of ventricular and vascular remodeling in heart failure (HF). Impaired endothelium-dependent relaxation caused by decreased endothelial NO has been demonstrated in hypertension and HF in humans and in experimental animals.1,2⇓ NO can be produced by essentially all cell types in the heart and is known to have profound effects on cardiac function. It is synthesized from l-arginine by the catalytic reaction of 3 different isoforms of NO synthase (NOS): neuronal or type 1 NOS (nNOS), inducible or type 2 NOS (iNOS), and endothelial or type 3 NOS (eNOS). nNOS and eNOS are constitutively expressed and Ca2+-dependent enzymes, whereas iNOS is essentially expressed in macrophages and leukocytes in response to appropriate stimuli. All 3 NOS isoforms are expressed in the heart.3 NO is a potent endogenous vasodilator that is responsible for the maintenance of basal vascular tone ...
Animals. The contractile performance was studied in five young (9-14 wk of age) male TR-α1-deficient mice and five wild-type control animals of the same age and weight (28-35 g). The force-frequency relationship (see below) was studied also in muscles from four female TR-α1-deficient mice and four wild-type control mice of the same age and weight. The TR-α1-deficient mice represent a cross between the SV-129/OLa and BALB/c (30). Contractile studies were performed on four male TR-β-deficient (12) and four control mice of the same age and weight as above. This group of mice has a mixed 129/Sv and C57Bl/6J genetic background, and was generated from TR-β+/ − heterozygote backcrosses. The wild-type mice were obtained from crosses of heterozygote TR-α1- or TR-β-deficient mice. The two homozygote wild-type strains were bred in parallel with the respective knockout strains. Thus the knockout strains have the same genetic background as their respective knockout strains: 129/Ola and BALB/c for ...
The purpose of this study was to investigate the hypothesis that cardiomyocyte-specific loss of the electrogenic NBCe1 Na+-HCO3- cotransporter is cardioprotective during in vivo ischemia-reperfusion (IR) injury. An NBCe1 (Slc4a4 gene) cardiac conditional knockout mouse (KO) model was prepared by gene targeting. Cardiovascular performance of wild-type (WT) and cardiac-specific NBCe1 KO mice was analyzed by intraventricular pressure measurements, and changes in cardiac gene expression were determined by RNA Seq analysis. Response to in vivo I/R injury was analyzed after 30 minutes occlusion of the left anterior descending artery followed by 3 hours of reperfusion. Loss of NBCe1 in cardiac myocytes did not impair cardiac contractility or relaxation and caused only limited changes in gene expression patterns, such as those for electrical excitability. However, following ischemia and reperfusion, KO heart sections exhibited significantly fewer apoptotic nuclei than WT sections. These studies indicate that
This work establishes an impact of cavin‐1 on pressure regulation in the pulmonary circulation. Right ventricular systolic pressure was robustly increased compared to WT littermate controls and accompanied by an increased right ventricular mass and remodeling of airway‐associated blood vessels. Because PAH is a progressive disease and we used younger animals, one may predict a more full‐blown PAH phenotype with aging. Since expression of caveolin‐1 was partly maintained, cavin‐1‐knockout mice may constitute a more adequate model of heritable PAH due to mutations in caveolin‐1 than do caveolin‐1‐knockout mice. This is because the disease‐causing mutations cause a partial reduction of caveolin‐1 (Austin et al. 2012), contrasting with the situation in caveolin‐1‐knockout mice where the protein is completely lacking.. In agreement with our current findings, thicker alveolar septa, hypercellularity, and elevated pulmonary pressure have been reported in ...
The WT Group provides clients with a single-source engineering solution to help maintain the integrity of all projects from start to finish. With nearly 50 years of experience, WT Groups highly skilled engineering, design and consulting teams ensure consistency, clarity, and accuracy in the most cost and time-efficient manner ...
Several protein-tyrosine phosphatases (PTPs) have been proposed to act as negative regulators of insulin signaling. Recent studies have shown increased insulin sensitivity and resistance to obesity in PTP1B knockout mice, thus pointing to this enzyme as a potential drug target in diabetes. Structure-based design, guided by PTP mutants and x-ray protein crystallography, was used to optimize a relatively weak, nonphosphorus, nonpeptide general PTP inhibitor (2-(oxalyl-amino)-benzoic acid) into a highly selective PTP1B inhibitor. This was achieved by addressing residue 48 as a selectivity determining residue. By introducing a basic nitrogen in the core structure of the inhibitor, a salt bridge was formed to Asp-48 in PTP1B. In contrast, the basic nitrogen causes repulsion in other PTPs containing an asparagine in the equivalent position resulting in a remarkable selectivity for PTP1B. Importantly, this was accomplished while retaining the molecular weight of the inhibitor below 300 g/mol. ...
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We study the molecular alterations of human rhabdomyosarcoma and other musculo-skeletal sarcomas, to dissect the mechanisms leading to unrestricted cell growth and to the inhibition of terminal differentiation. We have also developed a transgenic/knockout mouse that spontaneously develops pelvic rhabdomyosrcomas, allowing the study of early events in the natural history of ...
Transcription regulator protein BACH2 is a protein that in humans is encoded by the BACH2 gene. It contains a BTB/POZ domain at its N-terminus which forms a disulphide-linked dimer and a bZip_Maf domain at the C-terminus. Model organisms have been used in the study of BACH2 function. A conditional knockout mouse line called Bach2tm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens performed: - In-depth immunological phenotyping - in-depth bone and cartilage phenotyping GRCh38: Ensembl release 89: ENSG00000112182 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000040270 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Sasaki S, Ito E, Toki T, Maekawa T, Kanezaki R, Umenai T, Muto A, Nagai H, Kinoshita T, Yamamoto M, Inazawa J, Taketo MM, Nakahata T, Igarashi K, Yokoyama M (Aug 2000). "Cloning and expression of human B ...
I am seeking to hire an individual to conduct activities for a lab that is creating animal models of learning and memory -- generating knock-out constructs, as well as, utilizing molecular biology skills to perform Cloning, Plasmid and Phage Preparation, PCR, making cDNA Libraries, Library Screening, Southern and Northern Blot Analyses, Western Blotting. Good communication and organizational skills are a plus. A likely candidate would possess a B.S., M.S. or MD degree. I represent a leading bio-tech company with research facilities in New York and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. If you know anyone that might be interested please forward this to them or contact: Stan Saxton Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 E-Mail: sis at candseek.com ...