Nine additional BXD recombinant inbred (RI) strains have been developed from the F2 cross of C57BL/6J and DBA/2J mouse strains. A tenth line stopped breeding in the F12 generation. F20 generation breeding pairs from the nine surviving strains and an F12 pair from the extinct line were genotyped at 319 genetic markers (primarily microsatellites) spanning most of the genome. Where typing data were lacking, the established set of 26 BXD strains also were genotyped at these same loci. The availability of these additional nine strains enhances the value of the BXD RI set for analysis of complex phenotypic traits. The proportion of loci still segregating at the F20 generation was found to closely approximate expectation, suggesting that selection favoring the retention of heterozygosity is not a strong factor. However, the number of crossovers between adjacent markers was frequently less than predicted from consensus map distances. A significant deficiency of recombinants was observed on Chrs 3,
Johansson AC, Sundler M, Kjellen P, Johannesson M, Cook A, Lindqvist AK, et al. Genetic control of collagen-induced arthritis in a cross with NOD and C57BL/10 mice is dependent on gene regions encoding complement factor 5 and FcγRIIb and is not associated with loci controlling diabetes. Eur J Immunol 2001; 31: 1847-56 ...
chr12:113416669-113417101, - strand. Annotation of mouse strain DBA/2J genome assembly provided by GENCODE consortium. Distributed via Ensembl Release 92. Gene type: gene segment; Gene Name: Ighv1-67 ...
chr6:134677344-134692389, - strand. Annotation of mouse strain DBA/2J genome assembly provided by GENCODE consortium. Distributed via Ensembl Release 92. Gene type: protein coding gene; Gene Name: Erp27 ...
Good point from Anonymous - the DBA have had a handful of in-house design teams as members for a number of years - but these are being fazed out.. The news announced at the AGM is that it is the whole organisations which are joining the DBA. Rather than in-house design teams, it is the design buying and design management functions that are leading the relationship. We are forging relationships were they matter most to both our existing agency membership and on the new client side.. And as for the tough trading conditions, yes they are tough, but we were also pleased to report at the AGM that membership (not including the new client side businesses) is at the highest level that it has ever been - and double what it was 10 years ago.. It is good to see that more and more design agencies are benefiting from belonging to their trade association - which many see as more important in these tough times.. ...
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The Rho family GTPase Rac1 regulates cytoskeletal rearrangements crucial for the recruitment, extravasation and activation of leukocytes at sites of inflammation. Rac1 signaling also promotes the activation and survival of lymphocytes and osteoclasts. Therefore, we assessed the ability of a cell-permeable Rac1 carboxy-terminal inhibitory peptide to modulate disease in mice with collagen-induced arthritis (CIA). CIA was induced in DBA/1 mice, and in either early or chronic disease, mice were treated three times per week by intraperitoneal injection with control peptide or Rac1 inhibitory peptide. Effects on disease progression were assessed by measurement of paw swelling. Inflammation and joint destruction were examined by histology and radiology. Serum levels of anti-collagen type II antibodies were measured by enzyme-linked immunosorbent assay. T-cell phenotypes and activation were assessed by fluorescence-activated cell sorting analysis. Results were analyzed using Mann-Whitney U and unpaired Student
The use of regulatory or immature dendritic cells (DCs) as tools for modulating experimental rheumatoid arthritis is very recent. TNF-stimulated DCs have been shown to restore tolerance in experimental autoimmune encephalomyelitis and collagen-induced arthritis (CIA).. Objective: We investigated the capacity of short-term lipopolysaccharide (LPS)-stimulated DCs pulsed with type-II collagen (CII) to induce tolerance against established CIA.. Methods: Bone marrow-derived DCs were generated in the presence of GM-CSF. After CIA induction, mice were injected at day 35 with a single dose of 4- or 24-hour LPS-stimulated DCs that had been loaded with CII (CII/DCs, 4hLPS/CII/DCs or 24hLPS/CII/DCs). Arthritis progression was monitored by clinical and histologic evaluations.. Results Flow cytometry of 4hLPS/CII/DCs showed intermediate CD40 and CD86 expression, lower than that of 24hLPS/CII/DCs (fully mature) and higher than that of CII/DCs (immature). A functional assay showed that 4hLPS/CII/DCs display ...
The DNA binding activity of AP-1 and NF-kappaB are markedly increased in both CIA and RA. In CIA, activation of AP-1 and NF-kappaB precede both clinical arthritis and metalloproteinase gene expression. NF-kappaB expression correlated better than AP-1 with metalloproteinase expression.
Methods Arthritis was induced in male C57BL/6 wild-type (WT), LXRα−/−, LXRβ−/− and LXRα/β double KO mice by injection with type II collagen and treated with 30 mg/kg of the LXR agonist GW3965 or vehicle control. The mice were monitored for articular inflammation and cartilage degradation by scoring for clinical signs of arthritis and by histological examination of the joints.. ...
(a)-(c) Typical histological appearance of CIA mouse paws: (a) severe inflammatory cellular infiltration-synovitis and pannus formation; (b) bone and cartil
... - DBA Risk Management Ltd is a specialist firm of consultant engineers providing services in the area of Environmental, Health and Safety, and Risk Management services. DBA engineers have been engaged on many projects to ident... edie.net
Review Graduate Program details of DBA - Accounting Online from Capella University. |p||!--StartFragment--|Capella Education Company was named one of Forbes magazine’s “100 Most Trustworthy Companies in America” for 2014 (Forbes, 2014).|/p| |p|Develop the advanced skills and...
There is considerable evidence implicating tumor necrosis factor alpha (TNF-alpha) in the pathogenesis of rheumatoid arthritis. This evidence is based not only on the universal presence of TNF-alpha in arthritic joints accompanied by the upregulation of TNF-alpha receptors but also on the effects of neutralizing TNF-alpha in joint cell cultures. Thus, neutralization of TNF-alpha in vitro results in inhibition of the production of interleukin 1, which like TNF-alpha, is believed to contribute to joint inflammation and erosion. To determine the validity of this concept in vivo, the effect of administering TNF-neutralizing antibodies to mice with collagen-induced arthritis has been studied. This disease model was chosen because of its many immunological and pathological similarities to human rheumatoid arthritis. TN3-19.12, a hamster IgG1 monoclonal antibody to murine TNF-alpha/beta, was injected i.p. into mice either before the onset of arthritis or after the establishment of clinical disease. Anti-TNF
Abstract: To evaluate the effect of methotrexate on collagen-induced arthritis, micro-computed tomography (micro-CT) and histopathological analyses were used in male Wistar rats. Rats were divided randomly into three groups. Group 1 was treated with 0.9% saline, and groups 2 and 3 were boosted with type Ⅱ collagen. From day 21 to 42, groups 1 and 2 were orally treated with 0.9% saline and group 3 was orally treated with 1.5 mg/kg methotrexate. All rats were sacrificed at day 42 after the first collagen treatment. Micro-CT analyses showed bony parameters, such as bone volume and trabecular number, were decreased in group 2 compared to group 1, and these parameters were recovered in group 3. Histopathological examination and pathological parameter scoring showed that the knee joints of rats in group 2 had severe joint destruction, showing cartilage and bone erosion, enlarged cavities with inflammatory cell infiltration and activation of synovial fibroblasts. By contrast, these changes were ...
Mutational activation of the K-ras oncogene often occurs in human and mouse lung adenocarcinomas. Since K-ras p21 functions in trans-membrane signaling, we have investigated whether the amount of this protein in lung cell membranes is a variable that could influence lung tumorigenesis, either due to genetic differences or in response to tumor promoters. The six mouse strains assessed showed little difference in the total lung K-ras p21 after immunoprecipitation and immunoblotting. However, amount of ras p21 in the membrane fraction showed significant differences, with C57BL/6 and BALB/c having 3-5-fold more than NIH Swiss, AKR and DBA mice. Interestingly, a congenic AKR strain having the Ahr(b-1) Ah receptor allele from C57BL/6 mice (designated AKR.B6Ah) had high lung membrane K-ras p21 similar to that of C57BL/6. To test for possible changes related to lung tumor promotion, mice were treated with a promotional dose of TCDD (5 nmol/kg). After 48 h C57BL/6 lungs showed an increase in p21 in both ...
Introduction: The Vbeta12-transgenic mouse was previously generated to investigate the role of antigen-specific T cells in collagen-induced arthritis (CIA), an animal model for rheumatoid arthritis. This mouse expresses a transgenic collagen type II (CII)-specific T-cell receptor (TCR) beta-chain and consequently displays an increased immunity to CII and increased susceptibility to CIA. However, while the transgenic Vbeta12 chain recombines with endogenous alpha-chains, the frequency and distribution of CII-specific T cells in the Vbeta12-transgenic mouse has not been determined. The aim of the present report was to establish a system enabling identification of CII-specific T cells in the Vbeta12-transgenic mouse in order to determine to what extent the transgenic expression of the CII-specific beta-chain would skew the response towards the immunodominant galactosylated T-cell epitope and to use this system to monitor these cells throughout development of CIA. Methods: We have generated and ...
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
TY - JOUR. T1 - Early pro-inflammatory cytokine elevations in the DBA/2J mouse model of glaucoma. AU - Wilson, Gina N.. AU - Inman, Denise M.. AU - Denger-Crish, Christine M.. AU - Smith, Matthew A.. AU - Crish, Samuel D.. PY - 2015/9/17. Y1 - 2015/9/17. N2 - Background: Neuroinflammation-astrogliosis, microglial activation, and changes in cytokine signaling-is a prominent feature of neurodegenerative disorders. Glaucoma is a group of chronic neurodegenerative conditions that make up the leading cause of irreversible blindness worldwide. Neuroinflammation has been postulated to play a significant role in the pathogenesis and progression of glaucomatous neurodegeneration. Though much is known regarding inflammation in the eye in glaucoma, little is known about cytokine activity outside of the retina where pathologies develop early. Methods: We traced the primary visual projection from the eye to the superior colliculus (SC) in DBA/2J and DBA/2J.Gpnmb + (control) mice using the anterograde tracer ...
Decreased production of anti-CII antibodies in mice treated with anti-IL-23p19.At day 35, at the end of the experiment as shown in Figure 2A, serum was collecte
Y-320 is an orally active immunomodulator, and inhibits IL-17 production by CD4 T cells stimulated with IL-15 with IC50 of 20 to 60 nM. Y-320 also inhibits the production of IFN-γ and TNF-α by mouse CD4 T cells stimulated with IL-15, CXCL12, and anti-CD3 mAb. Y-320 (0.3-3 mg/kg p.o.) ameliorates collagen-induced arthritis (CIA) in Mice with a reduction of IL-17 mRNA in arthritic joints, and also shows therapeutic effects on CIA in cynomolgus monkeys. Moreover, Y-320 shows a synergistic effect in combination with anti-TNF-α mAb on chronic-progressing CIA in mice. "Selleck Chemicals Y-320, IL-17 Inhibitor". 26 Sep 2014. Ushio H, et al. (2013). "A new phenylpyrazoleanilide, y-320, inhibits interleukin 17 production and ameliorates collagen-induced arthritis in mice and cynomolgus monkeys". Pharmaceuticals (Basel). 7 (1): 1-17. doi:10.3390/ph7010001. PMC 3915191 . PMID 24366113 ...
Therapeutic Treatment of Arthritic Mice with 15-Deoxy Δ12,14-Prostaglandin J2 15d-PGJ2 Ameliorates Disease through the Suppression of Th17 Cells and the Induction of CD4 CD25−FOXP3 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
please send me resumes at [email protected] Microsoft SQL Server DBA Length: 3 months Start: ASAP Interview :phone The Technology Operations DBA Specialist is responsible for implementing timely, complex database technology solutions to multiple BPO customers during all phases of the capability release design, build, test and run of software. The Technology Operations DBA Specialist maintains the integrity of the test and production environments and ensures that those changes are managed through the lifecycle. The Technology Operations DBA Specialist executes database technology requests and serves as the ongoing escalation point for requests as necessary. The Technology Operations DBA Specialist works with Technology Operations Analysts and Specialists to resolve problems. Key Responsibilities: -Responsible for database administration, including table maintenance, backup and restore, database design and data integrity. -Implement standards and processes that facilitate stability of ...
As many of you know, the Daniella Maria Arturi Foundation (DMAF) has worked with the United States Department of Health and Human Services Centers for Disease Control and Prevention (CDC) for many years on efforts to improve DBA outreach, education and surveillance initiatives. Over the course of this effort CDC together with nurses and doctors from DBA resource centers and DBA patient groups have produced a series of helpful DBA fact sheets that are helping increase awareness of DBA and the steps to take to improve the quality of life for people living with DBA.. DMAF is excited to announce that CDC has continued to expand the resources available to the DBA community by developing a Spanish version of the DBA website, available at by clicking here!. We are very proud of the work everyone in the DBA community has done over the years to make materials available for families dealing with this rare condition. The DBA Care notebook and the accompanying factsheets are a welcomed source of knowledge, ...
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Collagen is the most abundant protein in the body, but you lose it as you age. Supplementing with bovine collagen can help, along with benefit your health.
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Report says interrogations were more brutal than CIA had said and it misled Congress and White House about its activities; agency disputes reports conclusions
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The purpose of this study was to investigate the lesions of a mouse collagen antibody-induced arthritis (CAIA) model using fluorescence bioimaging and micro-computed tomography (micro-CT) and to compare it with histopathological examination. Twelve mice were randomly divided into three groups: group 1 (G1) as control, group 2 (G2) as fluorescence probe control and group 3 (G3) as collagen antibody-induced arthritis. The mice of G3 intravenously received anti-type II collagen 5-clone antibody cocktail (2 mg/mouse) on day 0 and intraperitoneally received lipopolysaccharide (|TEX|$50{\mu}g/mouse$|/TEX|) on day 3. On the while, the mice of G1 and G2 received 0.9% saline in equal volumes at equivalent times. Fluorescence bioimaging and micro-CT analysis were carried out to assess arthritis. Treatment with the collagen antibody cocktail increased the paw thickness of mice compared to those in both the control and probe-treated groups. Fluorescence bioimaging using a near infrared imaging agent showed high
TY - JOUR. T1 - Anti-inflammatory effects of licorice and roasted licorice extracts on TPA-induced acute inflammation and collagen-induced arthritis in mice. AU - Chung, Won Yoon. AU - Kim, Ki Rim. AU - Jeong, Chan Kwon. AU - Park, Kwang Kyun. AU - Choi, Jong Hoon. AU - Park, Jung Han Yoon. AU - Lim, Soon Sung. PY - 2010/5/6. Y1 - 2010/5/6. N2 - The anti-inflammatory activity of licorice (LE) and roated licorice (rLE) extracts determined in the murine phorbol ester-induced acute inflammation model and collagen-induced arthritis (CIA) model of human rheumatoid arthritis. rLE possessed greater activity than LE in inhibiting phorbol ester-induced ear edema. Oral administration of LE or rLE reduced clinical arthritis score, paw swelling, and histopathological changes in a murine CIA. LE and rLE decreased the levels of proinflammatory cytokines in serum and matrix metalloproteinase-3 expression in the joints. Cell proliferation and cytokine secretion in response to type II collagen or ...
In collagen-induced arthritis (CIA) of mouse, self-reactive T cells recognize a peptide antigen from type II collagen (CII). CII is of particular interest, as an autoimmune response to this protein leads to CIA in mice, rats, and primates. Activation of T cells is believed to be an important pathogenic factor in autoimmune disease. So, T cells have become a focal point of study for the development of novel therapeutic approaches to the treatment of autoimmune disease. In this study, we evaluated the efficacy and mechanism of recombinant MHC II molecules in regulation of the antigen-specific T cell clones by using mouse I-Aq, combined with an auto-antigen peptide from type II collagen (CII260-274) in CIA model. It was found that recombinant I-Aq/CII260-274 molecules not only activate CII-specific T cell clone but also inhibit the same clone in vitro according to the condition of stimulation. Furthermore, development of CIA in mice was successfully prevented by the in vivo injection of a ...
CD4 T cells play an essential role in lupus pathogenesis by providing help to autoreactive B cells that, in turn, produce the pathogenic autoantibodies mediating tissue injury (29, 47). This study advances our understanding of the critical role of CD4 T cells by demonstrating that pre-existing differences in the inherent strength of CD4 T cell help for B cells can shape the severity of lupus expression. Using MHC-identical but background gene-disparate strains, we observed that, following an experimentally induced loss of tolerance, CD4 T cells from H-2d mice on the C57 black background induced transient autoantibodies without major lupus renal disease, whereas equivalent numbers of CD4 T cells from H-2d DBA mice induced prolonged increases in autoantibody and severe lupus nephritis. These striking differences in the CD4 lupus-inducing potential were associated with two contrasting patterns of in vivo CD4 T cell activation. Naive B10.D2 CD4 T cells induced a strong Th1/CMI response that was ...
A rapid and synchronized alternative to the CIA and K/BxN models that reduces variability, eliminates the need for expensive colonies and conserves test compound, controls and vivarium space.
Rhomboid family member 2 gene (Rhbdf2) is an inactive homologue lacking essential catalytic residues of rhomboid intramembrane serine proteases. The protein is necessary for maturation of tumor necrosis factor-alpha (TNF-α) converting enzyme, which is the molecule responsible for the release of TNF-α. In this study, Rhbdf2 knockout (KO) mice were produced by CRISPR/CAS9. To see the effects of the failure of TNF-α release induced by Rhbdf2 gene KO, collagen-induced arthritis (CIA), which is the representative TNF-α related disease, was induced in the Rhbdf2 mutant mouse using chicken collagen type II. The severity of the CIA was measured by traditional clinical scores and histopathological analysis of hind limb joints. A rota-rod test and grip strength test were employed to evaluate the severity of CIA based on losses of physical functions. The results indicated that Rhbdf2 mutant mice showed clear alleviation of the clinical severity of CIA as demonstrated by the significantly lower severity ...
IL-7 stimulated IL-7R+ mature B cells act proinflammatory (increased clinical score, increased anticollagen type II antibodies) after cell transfer in CIA. The sympathetic neurotransmitter norepinephrine abrogates this effect. Expression of IL-7Rα is increased when B cells are activated (anti-CD40 or lipopolysaccharide) in vitro and stimulating the IL-7R induces intracellular accumulation of pSTAT5. α- And β-adrenergic agonists show no influence on expression levels of IL-7R on activated B cells; however, intracellular IL-7R downstream signalling is abrogated via the β2-adreonceptor (β2AR) agonist terbutaline. IL-7R and β2AR are also expressed on B cells in synovial tissue from RA and OA patients ...
For these and other job titles, Dr. Seaborne says that that DBA can enhance or extend a career - even among those who already hold a masters of business administration (MBA).. "The DBA gives executives the opportunity to brush up on what they learned while taking their MBA courses 15 or 20 years ago. It also gives professionals the opportunity to take the next step in their career.". The right DBA program will prepare you to investigate and understand what current literature says about a topic, as well as help you understand how to apply current research-based theories to solve an actual organizational problem. In addition to improving upon research skills, the DBA can also help you refine your writing and presentation skills, making you a more clear and concise communicator.. In addition to career enhancement opportunities, the DBA can open you up to career or industry change as well.. "The difficulty or ease of transition really depends on which industry youre switching to," explains Dr. ...
Bovine collagen is a naturally occurring protein that is found in the skin, bones, and muscles of cows. This collagen is so similar to human collagen that many people in
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But quite often, for the actual, Real Application Monitoring we will also implement our own home-grown or OSS solution next to the GridControl. You generally need a tool where you can add/tweak your own monitoring, and that you can rely on to do (or build) the stuff that the commecial tools dont do, or dont want to do. And despite what the book, the courses, and the management say, any DBA or sysadmin worth his salt will DIY in this area. I am also still guilty of jotting up my own unix and sqlplus scripts in various situations. They will do Exactly what I need when I need it. Some of my unix/linux scripts have dates in them going back to 1994 (sad eh ...
But quite often, for the actual, Real Application Monitoring we will also implement our own home-grown or OSS solution next to the GridControl. You generally need a tool where you can add/tweak your own monitoring, and that you can rely on to do (or build) the stuff that the commecial tools dont do, or dont want to do. And despite what the book, the courses, and the management say, any DBA or sysadmin worth his salt will DIY in this area. I am also still guilty of jotting up my own unix and sqlplus scripts in various situations. They will do Exactly what I need when I need it. Some of my unix/linux scripts have dates in them going back to 1994 (sad eh ...
SICKENING details have emerged of the torture program set up by the CIA under former US president George W. Bush and the brutality goes beyond what anyone expected.
Kuznetsov, Gleb; Goodman, Daniel B.; Filsinger, Gabriel T.; Landon, Matthieu; Rohland, Nadin; Aach, John; Lajoie, Marc J. et al. (Genome Biology, 2017) Link to Published Version ...
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Kremowy żel do mycia ciała marki Sylveco delikatnie myje i pielęgnuje, wspomaga regenerację skóry oraz chroni przed wysuszeniem. Jest hypoalergiczny.