TY - JOUR. T1 - Enhancement of methanol production from synthetic gas mixture by Methylosinus sporium through covalent immobilization. AU - Patel, Sanjay K.S.. AU - Selvaraj, Chandrabose. AU - Mardina, Primata. AU - Jeong, Jae Hoon. AU - Kalia, Vipin C.. AU - Kang, Yun Chan. AU - Lee, Jung Kul. PY - 2016/6/1. Y1 - 2016/6/1. N2 - Both methane (CH4) and carbon dioxide (CO2) are major greenhouse gases (GHGs); hence, effective processes are required for their conversion into useful products. CH4 is used by a few groups of methanotrophs to produce methanol. However, to achieve economical and sustainable CH4 reduction strategies, additional strains are needed that can exploit natural CH4 feed stocks. In this study, we evaluated methanol production by Methylosinus sporium from CH4 and synthetic gas. The optimum pH, temperature, incubation period, substrate, reaction volume to headspace ratio, and phosphate buffer concentration were determined to be 6.8, 30 °C, 24 h, 50% CH4, 1:5, and 100 mM (with 20 ...

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Kok FN, Arica MY, Halicigil C, Alaeddinoglu G, Hasirci V Carboxymethylcellulose microspheres cross-linked via aluminum ions were used as a support material for immobilization of Methylosinus isolated from soil contaminated with aldicarb. The degradation capacity of immobilized bacteria, different parameters such as substrate concentration (50-800 ppm), flow rate (10-60 ml h−1), and continuous contact with reaction medium (flow rate, 20 ml h−1 and concentration, 100 ppm) that affect aldicarb degradation were investigated in a packed-bed reactor. Increases in the flow rate decreased the conversion of aldicarb into its metabolites. On the other hand, increasing the substrate concentration up to 400 ppm led to an increase in the amount of aldicarb converted (max 16%). Beyond this, the proportion of aldicarb that converted was decreased, reaching approximately 7% at 800 ppm. The apparent kinetic parameters, K′m and Vmax, were determined to be as 310.11 ppm and 2.29 × 10−2 ppm s−1, ...

Fusion porin protein of 1115 aas with two domains that are homologous to members of family 1.B.4 at the N- and C-termini of this protein and a central domain homologous to members of family 1.B.13 proteins. These fusion proteins are found in several closely related species of α-proteobacteria such as those in the genuses, Methylosinus and Methylocystis (BL Reddy and MH Saier, unpublished observations). ...

Plasmid pMo130-TelR from Dr. Kim Lee Chuas lab contains the insert tellurite-resistance cassette and is published in BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158. This plasmid is available through Addgene.

The PMO/ Project Support role is to support the project delivery teams. This will involve the coordination of key projects activities and provision of support in the Programme Management Office, Portfolio and Project...

BJPs attack on Congress got a boost after a note dated December 6, 2004 from the PMO showed the office being involved in appointment of Suresh Kalmadi as chief of CWG OC.

Carbon isotopic analysis of methane has become a popular technique in the exploration for oil and gas because it can be used to differentiate between thermogenic and microbial gas and can sometimes be used for gas-source rock correlations. Methane-oxidizing bacteria, however, can significantly change the carbon isotopic composition of methane; the origin of gas that has been partially oxidized by these bacteria could therefore be misinterpreted. We cultured methane-oxidizing bacteria at two different temperatures and monitored the carbon and hydrogen isotopic compositions of the residual methane. The residual methane was enriched in both 13C and D. For both isotopic species, the enrichment at equivalent levels of conversion was greater at 26??C than at 11.5??C. The change in ??D relative to the change in ??13C was independent of temperature within the range studied. One culture exhibited a change in the fractionation pattern for carbon (but not for hydrogen) midway through the...

Methanotrophs (sometimes called methanophiles) are prokaryotes that metabolize methane as their only source of carbon and energy. They can grow aerobically or anaerobically and require single-carbon compounds to survive. These Methanotrophs are currently being modified to absorb methane from the atmosphere, because methane released into the atmosphere contributes to greenhouse gasses, which heat up the earth and cause global warming. Under aerobic conditions, they combine oxygen and methane to form formaldehyde, which is then incorporated into organic compounds via the serine pathway or the ribulose monophosphate (RuMP) pathway. Type I methanotrophs are part of the Gammaproteobacteria and they use the RuMP pathway to assimilate carbon. Type II methanotrophs are part of the Alphaproteobacteria and utilize the Serine pathway of carbon assimilation. They also characteristically have a system of internal membranes within which methane oxidation occurs. Methanotrophs occur mostly in soils, and are ...

Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...

Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...

Organizations: Microbial Ecology Variables: flow_cytometric_cell_counts potential_methane_oxidation_rates Systems: freshwater lake Keywords: Lake pmoA gene Filter Results ...

|span||em||b|Career ladder associated to the role: level will be commensurate with the knowledge, skills and abilities of the successful...

18 Photos Of Cute Babies Tasting Lemons For The Very First Time. Their Reactions Are Hilarious! - viralscape-babies-tasting-lemon- ...

The particulate methane monooxygenase gene clusters, pmoCAB, from two representative type II methanotrophs of the alpha-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp, strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp, strain M, Immediately upstream of the putative start site, consensus sequences for sigma(70) promoters were identified, suggesting that these pmo genes are recognized by sigma(70) and negatively regulated under low-copper conditions. The pmo genes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia coil host. Methanotrophs contain two virtually identical copies of pmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB ...

TY - JOUR. T1 - Different Abilities of Eight Mixed Cultures of Methane-oxidizing Bacteria to Degrade TCE. AU - Broholm, Kim. AU - Christensen, Thomas Højlund. AU - Jensen, Bjørn K.. PY - 1993. Y1 - 1993. N2 - The ability of eight mixed cultures of methane-oxidizing bacteria to degrade trichloroethylene (TCE) was examined in laboratory batch experiments. This is one of the first reported works studying TCE degradation by mixed cultures of methane-oxidizing bacteria at 10°C, a common temperature for soils and groundwaters. Only three of the eight mixed cultures were able to degrade TCE, or to degrade TCE fast enough to result in a significant removal of TCE within the experimental time, when the cultures used methane as growth substrate. The same three mixed cultures were able to degrade TCE when they oxidized methanol, but only for a limited time period of about 5 days. Several explanations for the discontinued degradation of TCE are given. An experiment carried out to re-activate the ...

PMO News: Latest and Breaking News on PMO. Explore PMO profile at Times of India for photos, videos and latest news of PMO. Also find news, photos and videos on PM,

In 2004, 7-Eleven Inc. created a project management office (PMO), which continues today and with remarkable success. The PMO was initiated in the IT area of 7-Eleven, because more than 50 percent of the IT projects were failing to meet the schedule or budget objectives. Using and evangelizing mission statements, PMO goals, a PMO tag line, PMO objectives, best practices, and metrics were integral contributors to the success of the PMO. The PMOs establishment and associated key leaders were all about value creation--business-driven processes. The PMO was not about textbook answers, although in-depth research and reading were done. Goals included scalability, service excellence, process expertise; PMO creators became change agents and business innovators. In addition to the tactical aspects of building a successful PMO, the organization had to create a strategic culture that supported the PMO and its stakeholders. The success of the IT PMO was so remarkable that 7-Eleven Inc. decided to roll out the

Ttec is seeking an experienced IT PMO Project Manager to join our Global IT PMO team. Our global IT PMO Organization supports the Global IT Team, in providing services to the entire enterprise that encompasses over 40,000 users across our businesses aligned in four pillars - Customer Care, Technology Services, Strategy Services, & Growth Services.. What youll be doing :. The Position of IT - PMO Project Manager is a project role within the IT PMO Delivery Team. This position is critical to the success of the IT PMO Delivery Team, and has a solid career path into senior project leadership roles within the IT PMO.. This position manages the initiation, planning, execution (including managing and controlling), and closing of small to large projects within the IT PMO Managed Project Portfolio, following the defined IT PMO Project Delivery Lifecycle.. Key Role Responsibilities will include :. The following duties and responsibilities generally reflect the expectations of this position but are not ...

Methane (CH4) flux to the atmosphere is mitigated via microbial CH4 oxidation in sediments and water. As arctic temperaturesincrease, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is importantto predicting future CH4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), andpyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C,and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH4 oxidation activitywas measured in microcosm incubations containing sediments at all temperatures, with the highest CH4 oxidation potential of37.5 mol g1 day1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and ofthe 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13C-labeled DNA obtained bySIP demonstrated ...

Methanotrophic bacteria utilize methane as their sole source of carbon and energy. The first step in their metabolic pathway is the oxidation of methane to meth...

Variables: pressure fluorescence salinity electric_conductivity concentration Systems: lake Keywords: pmoA_RNA 16SrRNA Methanotroph pmoA_DNA Filter Results ...

All but the smallest IT organizations need a portfolio management office (PMO). IT will always have more work in its list than it can work on, and, given the complex operating environment of most.... ...

Arctic lakes are a significant source of the greenhouse gas methane (CH4), but the role that methane oxidizing bacteria (methanotrophs) play in limiting the overall CH4 flux is poorly understood. Here, we used stable isotope probing (SIP) techniques to identify the metabolically active aerobic methanotrophs in upper sediments (0-1 cm) from an arctic lake in northern Alaska sampled during ice-free summer conditions. The highest CH4 oxidation potential was observed in the upper sediment (0-1 cm depth) with 1.59 μmol g wet weight-1 day-1 compared with the deeper sediment samples (1-3 cm, 3-5 cm and 5-10 cm), which exhibited CH4 oxidation potentials below 0.4 μmol g wet weight-1 day-1. Both type I and type II methanotrophs were directly detected in the upper sediment total communities using targeted primer sets based on 16S rRNA genes. Sequencing of 16S rRNA genes and functional genes (pmoA and mxaF) in the 13C-DNA from the upper...

Lake sediments constitute an important compartment in the carbon cycle of lakes, by burying carbon over geological timescales and by production and emission of greenhouse gases. The degradation of organic carbon (OC) in lake sediments is linked to both temperature and oxygen (O-2), but the interactive nature of this regulation has not been studied in lake sediments in a quantitative way. We present the first systematic investigation of the effects of temperature on the apparent respiratory quotient (RQ, i.e., the molar ratio between carbon dioxide (CO2) production and O-2 consumption) in two contrasting lake sediments. Laboratory incubations of sediment cores of a humic lake and an eutrophic lake across a 1-21 degrees C temperature gradient over 157days revealed that both CO2 production and O-2 consumption were positively, exponentially, and similarly dependent on temperature. The apparent RQ differed significantly between the lake sediments (0.630.26 and 0.990.28 in the humic and the eutrophic ...

Methanotrophs are organisms that require methane as a source of carbon and energy for their metabolism. They are gram-negative bacteria that are capable in utilizing methane as a carbon energy source and able to grow both aerobically or anaerobically which only need single-carbon compound to live on. These prokaryotes occur mostly in soils, rice paddles, mud, landfills, and other places where methane is available. They can grow aerobically or anaerobically. In aerobic conditions, they use oxygen and methane to produce formaldehyde. They then incorporate it into organic compounds through ribulose monophosphate pathway or by serine pathway. An example of methanotroph is a gammaproteobacterium that utilize RuMP pathway in assimilating carbon. Such bacteria are referred to as type I methanotrophs. Type II methanotrophs are those from alphaproteobacteria that utilize the serine pathway of carbon assimilation. They have a system of internal membranes where methane oxidation occurs.1 Methanotrophs are ...

Kumke, T. , Hultzsch, N. , Schoonderwaldt, A. and Kienel, U. (2002): Spatial variability of lake sediment compositions - a case study from Lake Lama, Central Siberia , Terra Nostra ...

Bruckmeier, B.F.; Jüttner, I.; Schramm, K.W.; Winkler, R.; Steinberg, C.E.; Kettrup, A., 1997: PCBs and PCDD/Fs in lake sediments of Grosser Arbersee, Bavarian Forest, South Germany

Maputo: the company MOZAL from the Australian based BHPBilliton international group - one of the worlds largest producer of aluminum and one of the most important companies for the sustained development of the Mozambican economy - has established what is currently a 14 years long partnership with PMO

Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20% of greenhouse gas emissions. Methane is routinely flared in many industrial processes without harnessing any of its energy. The environmental impact and wasted energy potential make it highly desirable to find an economically feasible process to use this methane.One possibility is to convert methane into liquid fuels for transportation and energy generation. Current technologies to convert methane gas to liquid fuels (GTL) are complex, and the facilities are only economical at huge scales. Methane gas is very difficult to transport and store, so GTL plants must be located at the source of the methane, typically at large petroleum fields or refineries.Biological conversion of methane to liquid fuels is an attractive alternative to traditional GTL processes, as microbial oxidation of methane can produce liquid fuels (e.g. methanol) at ambient temperatures and ...

D-arabino-3-Hexulose 6-phosphate was prepared by condensation of formaldehyde with ribulose 5-phosphate in the presence of 3-hexulose phosphate synthase from methane-grown Methylococcus capsulatus. The 3-hexulose phosphate was unstable in solutions of pH greater than 3, giving a mixture of products in which, after dephosphorylation, allulose and fructose were detected. A complete conversion of d-ribulose 5-phosphate and formaldehyde into d-fructose 6-phosphate was demonstrated in the presence of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase (prepared from methane-grown M. capsulatus). d-Allulose 6-phosphate was prepared from d-allose by way of d-allose 6-phosphate. No evidence was found for its metabolism by extracts of M. capsulatus, thus eliminating it as an intermediate in the carbon assimilation process of this organism. A survey was made of the enzymes involved in the regeneration of pentose phosphate during C1 assimilation via a modified pentose phosphate cycle. On the basis ...

Fisher, O. S.; Sendzik, M. R.; Ross, M. O.; Lawton, T. J.; Hoffman, B. M.; Rosenzweig, A. C. PCuAC domains from methane-oxidizing bacteria use a histidine brace to bind copper. J. Biol. Chem. 2019, 294, 16351.. Kenney, G. E.; Dassama, L. M. K.; Manesis, A. C.; Ross, M. O.; Chen, S.; Hoffman, B. M.; Rosenzweig, A. C. MbnH is a diheme MauG-like protein associated with microbial copper homeostasis. J. Biol. Chem. 2019, 294, 16151. Ro, S. Y.; Schachner, L. F.; Koo, C. W.; Purohit, R.; Remis, J. P.; Kenney, G. E.; Liauw, B. W.; Thomas, P. M.; Patrie, S. M.; Kelleher, N. L.; Rosenzweig, A. C. Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase. Nat. Commun. 2019, 10, 2675.. Ross, M. O.; MacMillan, F.; Wang, J.; Nisthal, A.; Lawton, T. J.; Olafson, B. D.; Mayo, S. L.; Rosenzweig, A. C.; Hoffman, B. M. Particulate methane monooxygenase contains only monocopper centers. Science 2019, 364, 566-570.. Ross, M. O.; Fisher, O. S.; Morgada, M. N.; ...

Although multiple lineages are observed, CHIKV is relatively conserved genetically: envelope protein genes can share over 99.8% nucleotide sequence identity among isolates in the same lineage while isolates from different lineages can diverge by 4.4 to 15.5% (23). In amino acid sequence comparisons, the envelope proteins share 95 to 99.9% identity; e.g., IOL strain LR diverges from the Asian/American isolate CNR20235 by 4.49% in the E2 protein (11). On the other hand, even between closely related isolates, nsP3 may differ by more than 6% in amino acid sequences (23). Even though this diversity is relatively limited, particularly in the amino acid sequences, notable differences in pathogenesis and virulence between ECSA and Asian lineage isolates have been reported (10, 11). Furthermore, individual amino acid substitutions, even between closely related ECSA and IOL lineages, can impact in vitro measures of virulence, such as plaque size, cytopathic effect, and replication kinetics (24).. Here, we ...

Journal Publications. Choi, D.-W., R.C. Kunz, E.S. Boyd, J.D. Semrau, W.E. Antholine, J.-I. Han, J.A. Zahn, J.M. Boyd, A.M. de la Mora, and A.A. DiSpirito. 2003. The membrane-associated methane monooxygenase (pMMO) and pMMO-NADH:quinone oxidoreductase complex from Methylococcus capsulatus Bath. J. Bacteriol. 185: 5755 -5764.. Choi, D.-W, W. E. Antholine, Y.S. Do, Jeremy D. Semrau, C.J. Kisting, R.C. Kunz, D. Campbell, V. Rao, S.C. Hartsel, and A.A. DiSpirito. 2005. Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane associated methane monooxygenase on Methylococcus capsulatus Bath. Microbiology 151, 3417-3426.. Choi, D.-W, C.J. Zea, Y.S. Do, J.D. Semrau, W.E. Antholine, M.S. Hargrove, N.L. Pohl, E.S. Boyd, G. G. Geesey, S.C. Hartsel, P.H. Shafe, M.T. McEllistrem, C.J. Kisting, D. Campbell, V. Rao, A.M. de la Mora, and A.A. DiSpirito. 2006. Spectral, Kinetic, and thermodynamic properties of Cu(I) and Cu(II) binding by methanobactin from Methylosinus ...