Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of |99%. The best mutant increases ethylene oxidation activity by |5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industri Directed Evolution

My father-in-law was in the hospital with pneumonia when I heard Brad Mehldau at the Village Vanguard in late September, so I left my cell...

This pretty much sums up where Ive been at lately: I hear that people are worried about me. If I had the energy, Id be worried about me too. That said, I think Im doing pretty damn good for where Im at and what Im dealing with. Clarification: I dont just sit around and mope…

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Ive started getting stroppy again. Stupidly stroppy where I mope and put myself to bed early in a bad mood because of pretty much nothing - the little things like forgetting to put the green dustbin out on a Friday, or feeling fat and unattractive and miserable. The thing is, when I get myself in…

Ill admit it: Im one of those strange pet parents. Ive gone to great lengths to let the dogs know that my dad is ok, and the other day I caught myself trying to explain things to them like they would actually understand. Ive held the phone up for them so my dad could talk to them, and they could sniff the little speaker hole. To someone looking in from the outside, it probably looks completely ridiculous, but it seems to help a little bit. Buster mopes around for a while after a call, but eventually he recovers and goes back to gnawing on one of his favorite rawhides or to snuggling under 50 different blankets ...

Sorry about the detour:. Youve probably been rolling your eyes and sighing heavily, wondering why the heck Im yammering about Chemistry. Relax. It was just a long pass. Ill cut to the chase now:. Ladies, weve all seen the memes. The letters of notice. Basically, your royal is getting bumped off the flight in favor of soccer.. Ouch.. When you get dumped, abandoned, neglected, ignored, whatever you do, do not mope around. Yes, those eye rolls and labored sighs fall smack in this category.. So what if he treats you like part of the furniture? If Im going to be brutally honest, you probably rank lower than the furniture right now; at least the TVs getting conversation; at least the couch is getting physical contact; at least the footstool gets missed if its moved - in fact, when he asks where it is, thats probably the only hint youll get that he knows you still exist.. Theres hope yet! If you can make him watch Alejandro and his three mujeres, surely you can watch twenty-two hombres ...

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Jūra magazine was published in Lithuania since 1935. The international business magazine JŪRA MOPE SEA has been published since 1999, preserving the title and continuing the traditions of its predecessor. The three words of the title JŪRA MOPE SEA stand for the word SEA in Lithuanian, Russian and English languages. As of 2010 the magazine has been published in English, Chinese, Russian and Lithuanian. The title of the SEA magazine in Chinese is 海. The magazine in web site is www.jura.lt

Theres a common misconception that people with mental health do nothing all day except mope around their houses. Admittedly some people do exactly that, however not everyone does! Many use their illn...

Fired in disgrace, kids show host Randolph Smiley finds himself out on the street, while his replacement Sheldon Mopes, finds himself on the fast track to success with a new hit show as the proud purple rhino Smoochy. But things take a turn for the worst when Sheldon finds out that some of the people that he works with, and some he doesnt know hes working for, are all in it for the money. Meanwhile, Randolph is slowly turning insane with his only thoughts focusing on killing Smoochy and getting back to his life of luxury ...

Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...

Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...

I doesnt take a genius to figure out what will then happen to these Rent Backed Securities? Their value will fall to absolutely zero, any institutions or individuals stupid enough to have bought them will get wiped out and our friends (We are richer than you are) like Blythe Masters and Jamie Dimon at J.P. Morgan will still own the real estate and will still get their multi-million dollar year end bonuses, and they will kick bake a few hundred thousand in campaign contributions to the politicians that let them set this mess up in the first place. The banks themselves might face a few million or a billion dollars in fines for their manipulations but the criminals that set up and personally profit from the fraud will walk away scott free, or at the very worst will retreat to some non-extradition treaty county and set themselves up in new digs that would make The Great Gatsby blush ...

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Neither of them would be any good on a stag night. They are not guys guys. Bond would have more money than you, and he would attract more women than you, and he would be better than you at everything. He dates from a time and a mindset whereby heroes were our superiors. He would resent your presence and probably let you take a bullet meant for him. He would tut-tut at your incompetence and your inability to understand all the fine wines, and whether it is red or white wine that goes with fish.. Whereas Jason Bourne would probably try to not get you killed, or at least he would feel remorse if he failed, but hes not a barrel of laughs. He would mope around listening to the Arcade Fire and worrying. He belongs to an age in which heroes were like us, but faster and fitter. I dont want to go on a stag night with a morose loner.. Of the ladies, Eva Green is clearly psychotic, and will draw a man into no end of trouble; Julia Stiles is boring and mumsy.. Instead, I would much rather go on a stag ...

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1MTY: Crystal structures of the methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath): implications for substrate gating and component interactions.

We all know that todays job market stinks, and even if the rest of you dont, I do. Getting a job is a MAJOR pain in the tuckus at this point in economic history, and it isnt going to get any better for the foreseeable future. So does that mean we need to sit on our keisters and mope about how none of us have jobs? Heck no! Have you ever considered a job that would be a fun alternative to whining? Well look no further, have I got the job for you! Wait for it…. Ready for it?. Be a clown!. Yes, thats right, being a clown is one of the most enjoyable, rewarding experiences you can have. I mean, think about it! Clowns get to go to all sorts of parties… entertain hundreds of people a month… and act like a complete ass without having to worry about what other people think about you.. Being a clown is a great experience. Do you always make other people happy? Of course not! There are some people out there that have a pathological hatred of clowns, others a pathological fear. Those knerds are ...

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If your life is so miserable, honey, why did you marry him?. She paused as she conjured up an answer that wouldnt disgrace herself or disappoint her mother. I think I felt trapped. You and Dad were so proud when the Golden Child moved away … I didnt want to be the biggest failure in our family.. Now thats unfair, young lady; dont compare yourself to your sister. And you know your brother will always be the biggest failure in our family. Lets not forget that you were the one who wanted to get married so fast, not us. Her mother paused. Hes not actually slow is he?. No, Mom, Jesus! Hes not dumb, hes just … different. He gets a little nervous around other people.. Honey, youve always been a social butterfly, what in Gods name did you even find attractive about him? All he does is mope around reading, and he ignores the whole family when we get together. There is something not right about him. He doesnt talk about normal things.. Her husband wasnt a monster. He wasnt ...

Chelsea helps Nick up - theres no right way to grieve - everyone understands and supports you. Not everyone, Nick mopes (Sage has already headed back to her room) Its not Dr Sketchys fault that the treatment wasnt finished. Hes not sure Hilary will die - but its possible IF Gwen doesnt find a way to give her these drugs!. Back at GCM, Devon wants to override the drugs that are effecting Hilarys memory. Perhaps her favourite food or music will help. Thinking of Sharon, Dylan says that sometimes you have to tak ea step back - give the person you love time to heal. And what if they dont? Devon worries. Neil says thats it complicated. And painful - but Hilary needs to know what happened. Neil confides that he was furious at the betrayal. He didnt mean the nice things he said at the wedding - he wanted them to suffer, so followed the honeymooners to BVI. I was there when you fell. Why didnt Neil say anything before now? He hoped Hilary would remember by herself. Yes, it was Neil Hilary ...

Synonyms: 2,5-Anhydro-3,4-dideoxyhexitol [ACD/IUPAC Name]2,5-Anhydro-3,4-didesoxyhexitol [German] [ACD/IUPAC Name]2,5-Anhydro-3,4-didésoxyhexitol [French] [ACD/IUPAC Name]2,5-dihydroxymethyltetrahydrofuran203-239-0 [EINECS]Hexitol, 2,5-anhydro-3,4-dideoxy- [ACD/ ...

Methanotrophs (sometimes called methanophiles) are prokaryotes that metabolize methane as their only source of carbon and energy. They can grow aerobically or anaerobically and require single-carbon compounds to survive. These Methanotrophs are currently being modified to absorb methane from the atmosphere, because methane released into the atmosphere contributes to greenhouse gasses, which heat up the earth and cause global warming. Under aerobic conditions, they combine oxygen and methane to form formaldehyde, which is then incorporated into organic compounds via the serine pathway or the ribulose monophosphate (RuMP) pathway. Type I methanotrophs are part of the Gammaproteobacteria and they use the RuMP pathway to assimilate carbon. Type II methanotrophs are part of the Alphaproteobacteria and utilize the Serine pathway of carbon assimilation. They also characteristically have a system of internal membranes within which methane oxidation occurs. Methanotrophs occur mostly in soils, and are ...

Nominated Substances: Tetrahydrofuran. Nomination Date: 01/00/1979 Nominator: NIOSH Rationale: Concern as to occupational exposure, candidate chemical for NIOSH criteria document NTP Principles: Pre 1993 - 1, 8 ...

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Define moper. moper synonyms, moper pronunciation, moper translation, English dictionary definition of moper. intr.v. moped , mop·ing , mopes 1. To think gloomily and persistently about something; be dejected. See Synonyms at brood. 2. To move in a listless or...

The particulate methane monooxygenase gene clusters, pmoCAB, from two representative type II methanotrophs of the alpha-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp, strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp, strain M, Immediately upstream of the putative start site, consensus sequences for sigma(70) promoters were identified, suggesting that these pmo genes are recognized by sigma(70) and negatively regulated under low-copper conditions. The pmo genes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia coil host. Methanotrophs contain two virtually identical copies of pmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB ...

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Principal Investigator：TERAMOTO Keiko, Project Period (FY)：1996 - 1997, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Hygiene

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This invention provides a process for producing 4-hydroxybutanal in a yield of at least 87 percent by hydroformylation of allyl alcohol in a solvent at a temperature of about 20° C-120° C and a pressure of about 15-150 psi in the presence of a rhodium metal-phosphine complex hydroformylation catalyst. Also provided are additional processing procedures for recovering the 4-hydroxybutanal product and converting it into 1,4-butanediol and tetrahydrofuran.

The gamma-radiation induced oxidation of 1,4-dioxane, tetrahydrofuran and 1,3-dioxolane by hydrogen peroxide in neutral aqueous ...

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WE HAVE all seen the pictures and read the stories about Demi Moores marriage breakdown with Ashton Kutcher. Im assuming we have because pictures of a rake thin Moore being rushed into hospital have been splashed across newspapers and celebrity magazines all over the world. Meanwhile her errant ex-husband Kutcher has been living it up large in Brazil. It really is a tale of gender difference in how we deal with relationships which end - women mope, dont eat and men simply have fun.. Yes, I know that sounds incredibly sexist. But its true and I make no apologies for saying it. The difference here is that Demi Moore is almost 50. At her age she should be setting a better example to her daughters on how to deal with a break-up. If the reports are correct, she is way too old to be sniffing or smoking some nitrous oxide and acting out over some man - husband or not. What kind of message does that send out to her kids? That when your man leaves you break down to an unrecognisable heap? Yes, cry me ...

Durant: In London, it was such a team environment it was scary. You got the biggest stars in the game in one room -- global brands -- and you got so much sacrifice. James Harden, he had just come off of Sixth Man of the Year and you seen that he was ready to take the next step, and he sat the bench. He didnt complain, he didnt mope around, he was like, All right, were gonna get this gold medal, cause at the end nobody gonna care who was the leading scorer on this team. Anthony Davis was there. He was a rookie, he soaked up everything, and look at him now. We had Kobe Bryant, who went games without shooting the ball. [Andre] Iguodala came off the bench, Tyson Chandler -- we had so many great players and we played against some good teams. We had some close games, but nobody cared who took the shots, nobody cared who was the leader. Everybody had a voice. It was like, If we get this gold medal, then who cares? ...

George Clooney gunning for trouble. (Focus Features) The American Running time: 104 minutes MPAA rating: R (profanity, violence, sexual content, nudity) On the Web The American George Clooney, one of the worlds most gregarious movie stars in real life, has gone stoic on screen to the extent that its almost time to check for a pulse. After diving into such a deep trance in The Men Who Stare At Goats last year that it looked like he was auditioning for dream-state status in either Avatar or Inception, Clooney has emerged instead to mope his way through The American, a hit-man...