We describe an approach to accelerate the search for competitive inhibitors for carbohydrate-recognition domains (CRDs). Genetically encoded fragment-based-discovery (GE-FBD) uses selection of phagedisplayed glycopeptides to dock a glycan fragment at the CRD and guide selection of Synergistic peptide motifs adjacent to the CRD. Starting from concanavalin A (ConA), a mannose (Man)-binding protein, as a bait, we narrowed a library of 10(8) glycopeptides to 86 leads that share a consensus motif, Man-WYD. Validation of synthetic leads yielded Man-WYDLF that exhibited 40 50-fold enhancement in affinity over methyl alpha-D-mannopyranoside (MeMan). Lectin array Suggested specificity: Man-WYD derivative bound only to 3 out of 17 proteins-ConA, LcH, and PSA-that bind to Man. An X-ray structure of ConA.:Man-WYD proved that the trimannoside core and Man-WYD exhibit identical CRD docking; but their extra-CRD binding modes are significantly. different. Still, they have comparable affinity and se... ...
Kaur D, Pham H, Larrouy-Maumus G, Rivière M, Vissa V, Guerin ME, Puzo G, Brennan PJ, Jackson Met al., 2009, Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria., PLoS One, Vol: 4 BACKGROUND: Mycobacteria produce two unique families of cytoplasmic polymethylated polysaccharides -- the methylglucose lipopolysaccharides (MGLPs) and the methylmannose polysaccharides (MMPs) -- the physiological functions of which are still poorly defined. Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putative biosynthetic pathways. METHODOLOGY/PRINCIPAL FINDINGS: We report here on the characterization of the Rv1208 protein of Mycobacterium tuberculosis and its ortholog in Mycobacterium smegmatis (MSMEG_5084) as the enzymes responsible for the transfer of the first glucose residue of MGLPs. Disruption of MSMEG_5084 in M. smegmatis resulted in a dramatic decrease in MGLP synthesis directly attributable to the ...
alpha 1-4-mannosyltransferase: from Mycobacterium smegmatis; involved in methylmannose polysaccharide elongation; GDP-mannose is donor
Methylmannosides: Mannosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with methyl alcohol. They include both alpha- and beta-methylmannosides.
Magnetic resonance studies of concanavalin A: location of the binding site of alpha-methyl-D-mannopyranoside.: The longitudinal nuclear magnetic relaxation time
Vicia faba lectin contained 40-57% beta-conformation, 4-23% alpha-conformation along with random coil at pH 7.2 depending upon the analytical methods used. The percentage of beta-conformation increased with the addition of N-acetyl-D-glucosamine or methyl alpha-D-mannopyranoside. The structural transitions of V. faba lectin were affected by alkali at pH 9.6 and 10.6. Binding constants and free energy changes for the interaction between V. faba lectin and N-acetyl-D-glucosamine and methyl alpha-D-mannopyranoside were estimated at pH 7.2 using the c.d. and fluorescence methods. ...
1ONA: A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes.
Synthesis of p-Isothiocyanatophenyl 3-O-(3,6-Dideoxy-alpha-D-xylo-hexopyranosyl)-alpha-D-mannopyranoside.. Eklind, Karin; Garegg, Per J.; Gotthammar, Birgitta ...
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Fig. 2. Biofilm formation by E. coli cells expressing FimH variants. Each FimH variant is indicated by its expression plasmid. (A) Biofilm formation on a microtiter plate surface by cells expressing the K-12 fimH allele when grown in LB under static conditions. (B) Biofilm formation on a microtiter plate surface by cells expressing FimH variants when grown in LB under HDF conditions. Note that no biofilm is produced by the K-12fimH allele (pMAS1) under these conditions. (C) Quantification of biofilm formation as determined by the amount of crystal violet staining for each of the FimH variants. All experiments were performed in the presence and absence of methyl-α-d-mannopyranoside (α mm). Results are expressed as the average of four independent experiments (± standard deviation). ...
Dive into the research topics of Receptors for pea and lentil lectins on human lymphoid cells: Demonstration of distinct lectin‐defined cell subclasses. Together they form a unique fingerprint. ...
Page contains details about Concanavalin A/bovine serum albumin-incorporated LA-conjugated Tetronic® 1107 micelles aggregates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards. ...
MAMFKTLSFL FLLCFQIHGT VAADENSLFS FNGFVKSPSF DKRVALFGDS KLVNDSSLIQ LTDSVTRSVG RVVYKKPINL FKGKQRNSRS FSTHFSFSSM SNEIGDDVLA FVMVPSSLDL SLFGKKDNSS SALGFLSQYA KNETVVAVEF GISRTGNSAR ILVGRPESAE IRNLSFVGDL MMDNGRTLSC MIEYEASSKR MMVRFRKSGS VKLFDPFFSF SVDLAKLWKG GEVTVGLSSA NGNSSSKAHF LHSWRFEIRH PPPMWMHSVP LEPNEVSKEE ENGRGRSECT RKMLGALIFG AACGALGALL GLYLWTICRV RRSMAVVPEE CAIEQGKK ...
LCM of 7 and 8 is the smallest number among all common multiples of 7 and 8. The methods to find the LCM of 7, 8 are explained here in detail.
LCM of 2, 4, 6, 8 and 10 is the smallest number among all common multiples of 2, 4, 6, 8 and 10. The methods to find the LCM of 2, 4, 6, 8, 10 are explained here in detail.
It is unclear if buccal cell samples contain sufficient human DNA with adequately sized fragments for high throughput genetic bioassays. Yet buccal cell sample collection is an attractive alternative to gathering blood samples for genetic epidemiologists engaged in large-scale genetic biomarker studies. We assessed the genotyping efficiency (GE) and genotyping concordance (GC) of buccal cell DNA samples compared to corresponding blood DNA samples, from 32 Nurses Health Study (NHS) participants using the Illumina Infinium 660W-Quad platform. We also assessed how GE and GC accuracy varied as a function of DNA concentration using serial dilutions of buccal DNA samples. Finally we determined the nature and genomic distribution of discordant genotypes in buccal DNA samples. The mean GE of undiluted buccal cell DNA samples was high (99.32%), as was the GC between the paired buccal and blood samples (99.29%). GC between the dilutions versus the undiluted buccal DNA was also very high (greater than ...
It is unclear if buccal cell samples contain sufficient human DNA with adequately sized fragments for high throughput genetic bioassays. Yet buccal cell sample collection is an attractive alternative to gathering blood samples for genetic epidemiologists engaged in large-scale genetic biomarker studies. We assessed the genotyping efficiency (GE) and genotyping concordance (GC) of buccal cell DNA samples compared to corresponding blood DNA samples, from 32 Nurses Health Study (NHS) participants using the Illumina Infinium 660W-Quad platform. We also assessed how GE and GC accuracy varied as a function of DNA concentration using serial dilutions of buccal DNA samples. Finally we determined the nature and genomic distribution of discordant genotypes in buccal DNA samples. The mean GE of undiluted buccal cell DNA samples was high (99.32%), as was the GC between the paired buccal and blood samples (99.29%). GC between the dilutions versus the undiluted buccal DNA was also very high (greater than ...
Beneficial effects of Va and/or Zn supplementation of children in NE Thailand are described in a companion abstract. In the same study, blastogenic response (BR) of T-lymphocytes to concanavalin-A (ConA) and PPD were assayed in cultures containing mononuclear cells (MNC) or whole blood (WB). Methods were previously described. Children were previously vaccinated with BCG. BR to ConA of MNC or WB from children supplemented with VA, Zn, VA + Zn or placebo were similar. BR to PPD of MNC was higher in children receiving VA + Zn than placebo, but not in children supplemented with VA or Zn alone. Data indicate that children with suboptimal VA and Zn nutriture supplemented with , 2 times RDA of these nutrients showed enhanced cellular immunity to PPD. This observation is relevant to BCG immunization program and thus may benefit public health. ...
TY - JOUR. T1 - Recognition of septanose carbohydrates by concanavalin A. AU - Castro, Steve. AU - Duff, Michael. AU - Snyder, Nicole L.. AU - Morton, Martha D. AU - Kumar, C. V.. AU - Peczuh, Mark W.. PY - 2005/11/7. Y1 - 2005/11/7. N2 - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. AB - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. UR - http://www.scopus.com/inward/record.url?scp=27844440774&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=27844440774&partnerID=8YFLogxK. U2 - 10.1039/b509243d. DO - 10.1039/b509243d. M3 - Article. VL - 3. SP - 3869. EP - 3872. JO - Organic and Biomolecular ...
The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis in mice. In contrast to several other models for acute hepatic damage, ConA-induced injury is primarily driven by the activation and recruitment of T cells to the liver. Hen …
The adherent culture of rat thoracic aorta cells featured in this section was stained with Alexa Fluor 488 conjugated to the lectin concanavalin A, which selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues (primarily in the endoplasmic reticulum).
In the interpretation of glycan profiling patterns (i.e., glycan profiles) taken by lectin microarrays, I have summarized important things and procedures as follows.. 1. Some sort of normalization is absolutely necessary in comparing glycan profiles differentially. One of the most useful normalization methods is Average Normalization. In this case, all of the lectin signals are devided by the average of all lectins on the array, and for convenience, the values are then multiplied by 100. 2. And, the differences in glycan profiles are interpreted taking lectin binding characteristics and CV (coefficient of variation) into consideration. Usually the CV is less than 10% in a lot, and that of lot-to-lot variation gets a little bit bigger than this. Lectin binding specificity is not one-to-one relationship like an antigen-antibody reaction, but is fairly broader than that. So, we must be careful in the interpretation if other lectins with similar binding characteristics are reacting in the same way ...
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78069 avhandlingar från svenska högskolor och universitet. Avhandling: Svenska Arbetsgivareföreningen och arbetskraftsinvandringen 1945-1972.
H.R. 2586. To require an independent assessment and report of subversive activities of the Russian Federation, and for other purposes. In GovTrack.us, a database of bills in the U.S. Congress.