In response to replication stress, a phospho-signaling cascade is activated and required for coordination of DNA repair and replication of damaged templates (intra-S phase checkpoint). How phospho-signaling coordinates the DNA replication stress response is largely unknown. We employed state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) approaches to generate high-coverage and quantitative proteomic and phospho-proteomic profiles during replication stress in yeast, induced by continuous exposure to the DNA alkylating agent methyl methanesulfonate (MMS). We identified 32,057 unique peptides representing the products of 4,296 genes, and 22,061 unique phosphopeptides representing the products of 3,183 genes. 542 phosphopeptides (mapping to 339 genes) demonstrated an abundance change of ≥ 2-fold in response to MMS. The screen enabled detection of nearly all of the proteins known to be involved in the DNA damage response, as well as many novel MMS-induced phosphorylations. We ...
Suppliers List, E-mail/RFQ Form, Molecular Structure, Weight, Formula, IUPAC, Synonyms for (1-OXYL-2,2,5,5-TETRAMETHYL-3-PYRROLINE-3-METHYL) METHANETHIOSULFONATE (CAS No. 81213-52-7)
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This invention relates to carvedilol methanesulfonate, compositions containing this compound and methods of using carvedilol methanesulfonate to treat hypertension, congestive heart failure and angina.
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2-Propynyl methanesulfonate ≥99.5%, acid |200 ppm, H2O |100 ppm; CAS Number: 16156-58-4; Synonym: 2-Propinyl methanesulfonate, Methanesulfonic acid; Linear Formula: C4H6O3S; find Sigma-Aldrich-809993 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
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Alkylating agents induce cytotoxic DNA base-adducts. In this thesis I provide evidence to suggest that Saccharomyces cerevisiae Tpa1 protein is involved in DNA alkylation repair. Little is known about Tpa1 as a repair protein beyond the initial observation from a high throughput analysis indicating that deletion of TPA1 causes methyl methane sulfonate (MMS) sensitivity in Saccharomyces cerevisiae. Using purified Tpa1, I demonstrate that wild type Tpa1 repairs both single and double-stranded methylated DNA and mutation of the amino acid residues involved in cofactor binding abolishes Tpa1 DNA repair activity. In this thesis I also investigate genetic interaction of Tpa1. I also demonstrate that Tpa1 could complement alkB function and rescue the MMS sensitivity of alkB deficient E.coli strain, HK82. Experimental data proves that deletion of TPA1 along with base excision repair (BER) pathway DNA glycosylase MAG1 renders the tpa1 ∆ mag1 ∆ double mutant highly susceptible to methylation-induced ...
MMS induces diverse rII mutations from a wild-type background in bacteriophage T4. About 56% are base pair substitutions, about 30% are frameshift mutations, and the remainder is a miscellaneous set of rapidly reverting or leaky mutants of unknown composition; but deletions were not detected. MMS-induced forward mutation is sharply reduced by the mutations px and y, which also reduce ultraviolet, photodynamic and γ-ray mutagenesis and increase killing by all of these agents. Thus, many of the mutations arise via the T4 WXY system. The induction of G:C → A:T transitions was detected even in a px or y background using sensitive reversion tests, and the few forward rII mutations that were induced from this background also behaved like transition mutations. Thus, some MMS-induced mutations arise independently of the WXY system, perhaps as a result of the (rather weak) ability of MMS to alkylate the O6 position of guanine.. ...
Literature References: Prepn: O. C. Billeter, Ber. 38, 2015 (1905). Mutagenicity studies: T. Alderson, Nature 207, 164 (1965); J. B. Jenkins, Mutat. Res. 4, 90 (1967); A. P. Schalet, ibid. 49, 313 (1978). Review of carcinogenicity studies: IARC Monographs 7, 245-252 (1974). Review of comparative mutagenicity of EMS and methyl methanesulfonate, q.v.: S. Kondo, Environ. Sci. Res. 24, 743-785 (1981). ...
Pancreatic β-cells are able to repair themselves after some sublethal injuries in vitro. However, little is known of the nature of the repair mechanisms active in these cells. This study examined the expression of growth arrest and DNA damage-inducible genes gadd 153 and gadd 45 in pancreatic rat islets and in the clonal insulin secretory HIT-T15 cells. Rat pancreatic islets were exposed in vitro to the alkylating agents streptozocin or methyl methanesulfonate, or to the cytokine recombinant interleukin-1 β. Islet exposure to STZ or MMS reduced insulin release by 40-50% over the next 4 h, whereas exposure to rIL-1 β induced a 60% increase in insulin release over the same period. Both gadd 153 and gadd 45 mRNA were detectable in rat islets, and their levels were increased twofold after STZ exposure, whereas MMS induced a fivefold increase in gadd 153 and a twofold increase in gadd 45 mRNA. Islet exposure to rIL-1β did not affect the expression of gadd 153 or gadd 45. HIT cells exposed to STZ ...
Kundu, Chanakya et al " Adenomatous polyposis coli (APC) protein causes hypersensitivity of mouse embryonic fibroblast cell lines to DNA-alkylating agent methylmethane sulfonate through base excision repair pathway ." Cancer Research 67.9 Supplement (2007): 1961. Web. 21 Feb. 2018. ...
Forward genetic analysis using ethyl methanesulfonate (EMS) mutagenesis has proven to be a powerful tool in biological research, but identification and cloning of causal mutations by conventional genetic mapping approaches is a painstaking process
[150 Pages Report] Check for Discount on Global and Chinese 2-Deoxy-2,2-difluoro-D-ribofuranose-3,5-dibenzoate-1-methanesulfonate (CAS 134877-42-2) Industry, 2016 Market Research Report report by Prof Research. The Global and Chinese 2-Deoxy-2,2-difluoro-D-...
The reason why lots of herpes cases remain unattended is that some people do not have any noticeable indications. About 80 % of people carrying the virus are unaware of it resulting in the high rate of annual transmission. In his book Rare Earths Forbidden Cures, Dr. Your body has a lack of oxygen. A short flair last year responded to 3 days of mms treatment and all has been well for over one year. It is important to consume healthy meals to enhance the body immune system to help in fighting the virus. When advising a mineral cleansing program, a specialist might suggest both partners partake in the program due to the fact that there are high chances both partners are infected despite signs and symptoms visibility.. Research shows that 200 mg daily is efficient. But if theres one thing we all know about the U.S. Admittedly, however, there is a solution. The viruses dont actually disappear at all - they just go "underground" in the tissues of the body. That was a lot more tolerable for me. I ...
In a gene trap screen we recovered a mouse mutant line in which an insertion generated a null allele of the Brd4 gene. Brd4 belongs to the Fsh/Brd family, a group of structurally related proteins characterized by the association of two bromodomains and one extraterminal domain. Members of this family include Brd2/Ring3/Fsrg1 in mammals, fs(1)h in Drosophila, and Bdf1 in Saccharomyces cerevisiae. Brd4 heterozygotes display pre- and postnatal growth defects associated with a reduced proliferation rate. These mice also exhibit a variety of anatomical abnormalities: head malformations, absence of subcutaneous fat, cataracts, and abnormal liver cells. In primary cell cultures, heterozygous cells also display reduced proliferation rates and moderate sensitivity to methyl methanesulfonate. Embryos nullizygous for Brd4 die shortly after implantation and are compromised in their ability to maintain an inner cell mass in vitro, suggesting a role in fundamental cellular processes. Finally, sequence ...
I have one friend who is taking MMS just now for his cancer treatment. When he began to take MMS, his red hemoglobines decreased in number. Anyone who have...
Greetings all, Can anyone advise on the protocol for HSV2 using MMS tabs? Ive tried the protocol with CDS and had no luck. I tried looking up the John D....
Ayurveda, the traditional Indian system of medicine has given great emphasis to the promotion of health. Rasayana is one of the eight branches of Ayurveda which refers to rejuvenant therapy. It has been reported that rasayanas have immuno-modulatory, antioxidant and antitumor functions, however, the genotoxic potential and modulation of DNA repair of many rasayanas have not been evaluated. The present study assessed the role of Brahmarasayana (BR) on Ethyl methanesulfonate (EMS)-and Methyl methanesulfonate (MMS)-induced genotoxicity and DNA repair in in vivo mouse test system. The mice were orally fed with BR (5 g or 8 mg / day) for two months and 24 h later EMS or MMS was given intraperitoneally. The genotoxicity was analyzed by chromosomal aberrations, sperm count, and sperm abnormalities. The results have revealed that BR did not induce significant chromosomal aberrations when compared to that of the control animals (p |0.05). On the other hand, the frequencies of chromosomal aberrations induced by
TY - JOUR. T1 - Analysis of heat-labile sites generated by reactions of depleted uranium and ascorbate in plasmid DNA. AU - Wilson, Janice. AU - Young, Ashley. AU - Civitello, Edgar R. AU - Stearns, Diane M. PY - 2014. Y1 - 2014. N2 - The goal of this study was to characterize how depleted uranium (DU) causes DNA damage. Procedures were developed to assess the ability of organic and inorganic DNA adducts to convert to single-strand breaks (SSB) in pBR322 plasmid DNA in the presence of heat or piperidine. DNA adducts formed by methyl methanesulfonate, cisplatin, and chromic chloride were compared with those formed by reaction of uranyl acetate and ascorbate. Uranyl ion in the presence of ascorbate produced U-DNA adducts that converted to SSB on heating. Piperidine, which acted on DNA methylated by methyl methanesulfonate to convert methyl-DNA adducts to SSB, served in the opposite fashion as U-DNA adducts by decreasing the level of SSB. The observation that piperidine also decreased the gel shift ...
Buy and view Colistin sodium methanesulfonate, to permeabilize membranes/mannose-resistant haemagglutination, 1264-72-8, MSDS. Click to view prices an
1BTP: Unique binding of a novel synthetic inhibitor, N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate, to bovine trypsin, revealed by the crystal structure of the complex.
Yes, I have tried MMS. I read all about it first, mainly from Jim Humblew website, whose website seems to be the origin & creation of MMS. I thought Id give it a go....I agreed to do it with someone who was terminally ill, as a way of giving them moral & encouragement, so that they didnt feel they were going it alone, as it were. So we both started a course of MMS together...I got up to 7 drops & had two bouts of serious nausea, that I can only describe as psychological/mental nausea, it lasted most of the day, & was most unpleasant. I dropped down a drop, of MMS & continued, though did not proceed further than 7 drops as the nausea was putting me off big time...this was an apparent indication it was working. I cant realy say I gave it a fir trial though. Also the friend who tried it, was also having chemotherapy aswell, so it wasnt really a clear indication of its failure or success. She made great progress in her healing, though sadly not a full recovery. The chemo led to further ...
The tumour suppressor protein p53 is a critical protein in the DNA damage checkpoint pathway. It acts as a transcription factor that is involved in initiating the apoptotic pathway; disruption of this pathway can lead to various forms of cancer. Due to its importance in cancer prevention, p53 has been extensively studied, though only a small fraction of these studies have been in non-mammalian models. Some previous studies of p53 expression and regulation in lower vertebrate species have shown potential differences in its control, in comparison to the better characterized mammalian pathways. These differences emphasize the need to further investigate its mechanism of action in lower vertebrate models. To evaluate its biomarker potential for aquatic toxicology studies, two rainbow trout cell lines (RTbrain-W1 and RTgill-W1) were used in dose response experiments using DNA damage checkpoint inducing agents: bleomycin, hydroxyurea, and methyl methanesulfonate. For our studies, a rainbow trout ...
Why should yeast have a specific IMP 5-nucleotidase? One possibility is that Isn1p could be involved in scavenging IMP toxic derivatives or analogs. Our attempts to document such an effect were unsuccessful : the isn1 knock-out mutant, grown in the presence of several purine analogs (8-azaadenine, 6-chloropurine, 8-azaguanine, 6-mercaptopurine, 2,6-diaminopurine...), did not show any growth alteration compared to the isogenic wild-type strain (data not shown). Alternatively, Isn1p could play some role in DNA repair. Indeed, transcriptome analysis revealed that ISN1 transcription increased three folds when cells were treated with methyl methanesulfonate, a DNA damaging agent [17], and furthermore, Isn1p co-purified with Mlh1p [18], a protein involved in mismatch repair [19]. It is therefore tempting to propose that Isn1p could for example be involved in removing dIMP residues resulting from deamination of dAMP. However, while the mutagenic effect of dIMP has been documented [20], this ...
Complete information for MMS22L gene (Protein Coding), MMS22 Like, DNA Repair Protein, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Hey everyone, I thought it important to share what things to look out for while doing your protocol...very important for speedy recovery. Taken from "" ...
Here, we demonstrate that OTUD4 may serve as a master regulator of alkylation damage resistance through stabilization of the human AlkB homologues. A number of distinct lines of evidence support this role for OTUD4. First, OTUD4 interacts specifically with ALKBH2 and ALKBH3 and encodes a K48‐specific DUB (Fig 1). Consistently, ALKBH3 is subjected to K48‐linked ubiquitination and proteasomal degradation (Fig 2A-D). OTUD4 antagonizes ALKBH3 ubiquitination and stabilizes both ALKBH2 and ALKBH3 in vivo (Fig 2E-H). ALKBH3 protein levels do not correlate well with ALKBH3 mRNA levels in various tumor cell lines but do correlate with OTUD4 levels (Supplementary Fig S2). Finally, overexpression of ALKBH3 in PC‐3 cells, which depend primarily on ALKBH3 instead of ALKBH2 for alkylation damage resistance, is sufficient to rescue alkylation damage sensitivity upon loss of OTUD4 (Fig 7G).. What is most striking is that we find OTUD4 catalytic activity to be apparently dispensable for its stabilization ...
The ubiquitin E1, E2, and E3 ligase enzymes are the enzymatic core of the ubiquitination pathway. The E2-E3 complex interacts with the substrate, catalyzing ubiquitin addition to the substrate. Thus, the expression, activity, localization, and selectivity of ubiquitin E2s and E3s are important parameters that can serve to regulate ubiquitination. The E2-E3 combination used in the ubiquitination reaction can also influence the fate of the substrate through determining the extent and nature of ubiquitin addition. The majority of substrates observed to date are modified by the attachment of a Lys-48-linked ubiquitin chain, which targets the substrate for degradation by the 26S proteasome. Substrates modified with other types of polyubiquitin chains linked via ubiquitin Lys-6, -11, -29, and -63, or modified with a single ubiquitin, face different or unknown fates. For example, the heterodimeric E2 UBC13/methyl methane sulfonate sensitivity 2 functions with the RING E3 TNF receptor-associated factor ...
Weller, R. (2004): Post-depositional losses of methane sulfonate, nitrate, and chloride in Antarctic firn. , Blockseminar (Environmental Physics - University of Bremen & AWI Bremerhaven) 05 July 2004 Alfred-Wegener-Institut für Polar- und Meeresforschung Building F (glass hall seminar room), Bussestraße 24, Bremerhaven Topic: Interactions between Atmosphere, I ...
Restructured from Jilantai Dakang Company in Alashan Prefecture, Dakang Fine Chemical Stock Company Limited was founded in 1993.Company is now energetically developing the fine chemical industry by successively investing such fine chemical products as tri
MMS_connectTarget = "host=localhost dbname=mmtnsage user=mmtnsage password=b33t!chip"; $MMS_dbConnect = "host=localhost dbname=mmtnsage user=mmtnsage password=b33t!chip"; $MMS_db_host = "localhost"; $MMS_db_name = "mmtnsage"; $MMS_db_user = "mmtnsage"; $MMS_db_passd = "b33t!chip"; $SageAuth_connectTarget = "host=localhost dbname=sage_auth user=mmtnsage password=b33t!chip"; $SageAuth_dbConnect = "host=localhost dbname=sage_auth user=mmtnsage password=b33t!chip"; $SageAuth_db_host = "localhost"; $SageAuth_db_name = "sage_auth"; $SageAuth_db_user = "mmtnsage"; $SageAuth_db_passd = "b33t!chip ...
The instructions given here are for the original, and what I like to call, Classic Protocol 1000. If you do further research you will find that we and many other people have tried various versions of Protocol 1000 over the years. While most all of the slight variations of Protocol 1000 have been successful, according to reports we have received from around the world the success has never been as good as the original protocol of mixing the dose fresh every hour. Protocol 1000 is taking a maximum of 3 drops of activated MMS (MMS1) in 4 ounces/120 ml of water once each hour, for eight consecutive hours, every day, for three weeks or until well. Do not start out at 3 drops an hour. Try to build up to 3 drops slowly. If your body does not tolerate an increase of drops, stick with what works best for you. Your health may be recovered taking less than 3 drops in each dose. Some people have recovered their health taking 1 or 2 drops per hour, and in rare cases even less. It is best to start out slow and ...
Updating A Managed Metadata (MMS) Column Dynamically In A SharePoint List Using MS Flow9/19/2019 8:20:20 AM. When updating an MMS Column in a SharePoint list, the value of the MMS column is expected to be in a certain format. This article explains the right way of updating these columns from MS Flow by ident ...
DNA dsb represent a severe type of damage that can be provoked in cell nuclei by exposure to ionizing radiation and also to chemicals such as etoposide, methylmethane sulphonate and bleomycin. Both radiation and chemical agents induce a variety of other DNA lesions such as base and sugar modifications, and single-strand breaks mostly mediated by the formation of highly reactive oxygen radicals (Iliakis, 1991). Although dsb are perhaps the lesions that most strongly threaten cell survival, it is likely that damaged cells seek to repair all DNA lesions produced by the oxygen radicals, thus activating several DNA repair pathways (Wood, 1996). Therefore, by using these DNA damaging agents, it might be difficult to analyze exclusively the repair of DNA dsb. On the other hand, it is desirable to have an in vitro system to study the repair of dsb produced in an environment as close as possible to the cell nucleus.. We used prokaryotic restriction enzymes (REs) in order to introduce solely dsb in the ...
1P7M: Solution structure and base perturbation studies reveal a novel mode of alkylated base recognition by 3-methyladenine DNA glycosylase I
A loading dose of 9 MU CMS was administered in 19 critically ill patients not on renal replacement with Gram-negative infections, achieving average colistin A plus B concentrations above 2 mg/liter within the first hours of treatment initiation. Moreover, in this study a novel structural model was developed in order to depict the delay in colistin formation. In addition, an interesting finding is that in comparisons of this study with previous studies in the same patient population (6, 7), higher plasma concentrations of colistimethate A plus B and, subsequently, colistin A plus B were apparent in the current CMS formulation.. In a population analysis of 18 critically ill patients receiving a dosage of 3 MU CMS every 8 h, a 48-h period was shown to be necessary for a typical patient in order to obtain steady-state plasma colistin concentrations (6), signifying a major delay in appropriate treatment. The latter observation is of high importance, as the delayed initiation of appropriate ...
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DNA probes prepared from human O6-methylguanine-DNA methyltransferase complementary DNA were hybridized to mRNA isolated from human liver and fifteen human tumor cell lines proficient (Mer+) or deficient (Mer-) in transferase activity. Liver and Mer+ cells contained levels of transferase-specific mRNA that correlated with their transferase activity levels, whereas Mer- cells contained undetectable amounts of transferase mRNA. The mRNA levels were not induced in human cells by treatments that induce other DNA damage-inducible genes. These results demonstrate that in human cells the transferase gene is constitutively expressed, that its expression is related to activity levels, and that in Mer- tumor cells the expression of the transferase gene is probably blocked at the level of mRNA production.. ...
MMS_connectTarget = "host=localhost dbname=mmtnsage user=mmtnsage password=b33t!chip"; $MMS_dbConnect = "host=localhost dbname=mmtnsage user=mmtnsage password=b33t!chip"; $MMS_db_host = "localhost"; $MMS_db_name = "mmtnsage"; $MMS_db_user = "mmtnsage"; $MMS_db_passd = "b33t!chip"; $SageAuth_connectTarget = "host=localhost dbname=sage_auth user=mmtnsage password=b33t!chip"; $SageAuth_dbConnect = "host=localhost dbname=sage_auth user=mmtnsage password=b33t!chip"; $SageAuth_db_host = "localhost"; $SageAuth_db_name = "sage_auth"; $SageAuth_db_user = "mmtnsage"; $SageAuth_db_passd = "b33t!chip ...
Melphalan is a DNA alkylating agent; induces cytotoxicity through the formation of stable interstrand and intrastrand crosslinks within DNA which inhibits growth of PC-3 cells (IC50 values are 0.074 and 0.77 μM for sequential dosing and single dosing respectively). Learn More ...
Im on 10mg of prednisone per day for my asthma. Ive recently started mms and when taking a full blood count test, it showed unsuppressed. Could mms cause...
... definition, any of various potentially cytotoxic, carcinogenic, and mutagenic substances: used therapeutically to destroy cells, especially proliferating cancer cells. See more.
Now while we women take our B-100, theres also a little something for you men who are married to women who suffer from MMS. Just ask my husband. A few years back he downloaded an app (P-Calendar on AppBox) that WARNS him when my MMS is about to hit. Can you EVEN BELIEVE such a thing EXISTS?! I dont know if I should be insulted or empowered by that. What I DO know, is that this app helps him know which nights hes better off working late. Like wait-till-shes-in-bed-ASLEEP late. ...
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Phytoplasmas are mycoplasma-like pathogens of witches broom disease, and are responsible for serious yield losses of Paulownia trees worldwide. The molecular mechanisms of disease development in Paulownia are of considerable interest, but still poorly understood. Here, we have applied transcriptome sequencing technology and a de novo assembly approach to analyze gene expression profiles in Paulownia fortunei infected by phytoplasmas. Our previous researches suggested that methyl methane sulfonated (MMS) could reverse the effects of the infection. In this study, leaf samples from healthy, infected, and both infected and methyl methane sulfonate treated plants were analyzed. The results showed that the gene expression profile of P. fortunei underwent dramatic changes after Paulownia witches broom (PaWB) phytoplasma infection. Genes that encoded key enzymes in plant-pathogen interaction processes were significantly up-regulated in the PaWB-infected Paulownia. Genes involved in circadian rhythm and
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Involvement of two endonuclease III homologs in the base excision repair pathway for the processing of DNA alkylation damage in Saccharomyces cerevisiae.
Base excision repair (BER) is the predominant DNA damage repair pathway for the processing of small base lesions, derived from oxidation and alkylation damages. BER is normally defined as DNA repair initiated by lesion-specific DNA glycosylases and completed by either of the two sub-pathways: short-patch BER where only one nucleotide is replaced and long-patch BER where 2-13 nucleotides are replaced. Each sub-pathway of BER relies on the formation of protein complexes that assemble at the site of the DNA lesion and facilitate repair in a coordinated fashion. This process of complex formation appears to provide an increase in specificity and efficiency to the BER pathway, thereby facilitating the maintenance of genome integrity by preventing the accumulation of highly toxic repair intermediates ...
It is an alpha-1-adrenergic blocking agent that is used for treatment of arterial hypertension and of obstructive symptoms resulting from benign prostatic hypertrophy. Oral administration once a day. Its pharmacokinetic data (moderately elevated molecular weight, high percentage of plasma protein binding and high volume of distribution) (Pfizer 2017, Kirsten 1998, Elliot 1987) probably explain the negligible excretion observed in milk (Pfizer 2017, Versmissen 2016, Jensen 2013 y 2014). Until more extensive published data about this drug regarding breastfeeding are available a safer alternative drug may be used (Anderson 2018, Schaefer 2007 p685), especially during the neonatal period and/or in case of premature infants.