The crystal structure of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaeon Methanothermus fervidus has been solved in the holo form at 2.1 a resolution by molecular replacement. Unlike bacterial and eukaryotic homologous enzymes which are strictly NAD(+)-dependent, GAPDH from this organism exhibits a dual-cofactor specificity, with a marked preference for NADP(+) over NAD(+). The present structure is the first archaeal GAPDH crystallized with NADP(+). GAPDH from M. fervidus adopts a homotetrameric quaternary structure which is topologically similar to that observed for its bacterial and eukaryotic counterparts. Within the cofactor-binding site, the positively charged side-chain of Lys33 decisively contributes to NADP(+) recognition through a tight electrostatic interaction with the adenosine 2-phosphate group. Like other GAPDHs, GAPDH from archaeal sources binds the nicotinamide moiety of NADP(+) in a syn conformation with respect to the adjacent ribose and so belongs to ...
1CF2: Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus.
The study is focused on the comparison of autothermal thermophilic aerobic digestion, thermophilic and mesophilic anaerobic digestion, based on long term monitoring of all processes in full-scale wastewater treatment plants, with an emphasis on the efficiency in destroying pathogens. The hygienisation effect was evaluated as a removal of counts of indicator bacteria, thermotolerant coliforms and enterococci as CFU/g total sludge solids and a frequency of a positive Salmonella spp. detection. Both thermophilic technologies of municipal wastewater sludge stabilisation had the capability of producing sludge A biosolids suitable for agricultural land application when all operational parameters (mainly temperature, mixing and retention time) were stable and maintained at an appropriate level. ...
ID METFV_1_PE97 STANDARD; PRT; 454 AA. AC METFV_1_PE97; E3GX13; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Anthranilate synthase, component I; EC=4.1.3 27; DE (METFV_1.PE97). GN OrderedLocusNames=Mfer_0098; OS METHANOTHERMUS FERVIDUS DSM 2088. OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanothermaceae; Methanothermus. OX NCBI_TaxID=523846; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS METFV_1.PE97. CC Methanothermus fervidus DSM 2088 chromosome, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:E3GX13_METFV CC -!- GENE_FAMILY: HOG000025142 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; E3GX13; -. DR EMBL; CP002278; ADP76902.1; -; Genomic_DNA. DR RefSeq; YP_004003664.1; NC_014658.1. DR GeneID; 9961809; -. DR GenomeReviews; CP002278_GR; Mfer_0098. DR KEGG; mfv:Mfer_0098; -. DR GO; GO:0004049; F:anthranilate synthase activity; ...
Foaming incidents have been recorded in many biogas plants causing severe operational, economical and environmental problems (Kougias et al., 2014). However, the foaming phenomenon in biogas reactors fed with agro-industrial wastes has not been extensively investigated, especially with respect to the microbial composition of the digesters (Moeller et al., 2012). In the cited literature, it has been reported that specific microorganisms, which are mainly filamentous (e.g. Gordonia species, Microthrix parvicella), are attached to biogas bubbles and transferred to the air/liquid interface of sludge reactors or wastewater treatment works (Ganidi et al., 2009). Once these microorganisms accumulate on the liquid surface, they initiate biosurfactants production due to their metabolic activity, leading to the decrease of the surface tension and thus generate foaming. The aim of the present study was to investigate the microbial diversity in the liquid versus the foaming layer in manure-based biogas ...
Anderson,I., Djao,O.D., Misra,M., Chertkov,O., Nolan,M., Lucas,S., Lapidus,A., DelRio,T.G., Tice,H., Cheng,J.F., Tapia,R., Han,C., Goodwin,L., Pitluck,S., Liolios,K., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A., Brambilla,E., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Sikorski,J., Spring,S., Rohde,M., Eichinger,K., Huber,H., Wirth,R., Göker,M., Detter,J.C., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Klenk,H.P., Kyrpides,N.C. ( 2010 ) Complete genome sequence of Methanothermus fervidus type strain (V24S) Stand.Genomic Sci 3 (3) :315-324 ...
The processes of N mineralization and immobilization which can occur in agricultural soils during decomposition of plant residues are briefly reviewed in this paper. Results from different incubation
Discover Lifes page about the biology, natural history, ecology, identification and distribution of Bombus fervidus, abdomen segment 4 light, UGCA195772 image
Kathiresan S., Willer CJ., Peloso G., Demissie S., Musunuru K., Schadt E., Kaplan L., Bennett DA., Li Y., Tanaka T., Voight BF., Bonnycastle LL., Jackson AU., Crawford G., Surti A., Guiducci C., Burtt N., Parish S., Clarke R., Zelenika D., Kubalanza KA., Morken MA., Scott LJ., Stringham HM., Gilan P., Swift AJ., Kuusisto J., Bergman RN., Laakso M., Ferrucci L., Scheet PA., Sanna S., Uda M., Yang Q., Lunetta K., Dupuis J., deBakker PI., ODonnell CJ., Chambers J., Kooner J., Hercberg S., Meneton P., Lakatta M., Scuteri A., Schlessinger D., Tuomilheto J., Collins FS., Groop L., Altshuler D., Collins R., Lathrop GM., Melander O., Salomaa V., Peltonen L., Orho-Melander M., Ordovas J., Boehnke M., Abecasis G., Mohlke K., Cupples LA ...
The study was conducted on eighteen postpartum silent oestrus Surti buffaloes to evaluate the efficacy of Norgestomet ear implants alone and in combination with PGF2α. The buffaloes in Group-I & Group-II were implanted with Crestar for 9 days along with 2 ml injection of Crestar solution given on the day of implant insertion. In Group-II, additionally 500 μg Cloprostenol was given on one day before implant removal. Whereas, the buffaloes in Group-III served as silent oestrus control group and 5 ml normal Saline was given on 0 day and on 8th day as a placebo treatment. In the Norgestomet treated groups the significant decreasing trend of endogenous progesterone (P4) concentration observed at different time intervals of ear implant inserted followed by Norgestomet injection given treatment groups of animals. Oestradiol-17β levels of the blood serum did not show any significant difference (p ,0.05) at 0 day, 5th day within the Group-I, Group-II & Group-III. But the mean values of ...
Lineage: cellular organisms; Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter; Methanobrevibacter ...
Bacteria, viruses, and other microorganisms.. Ground-water infiltration from agricultural lands to which nutrients have been applied may transport the following pollutants: soluble nutrients and chemicals, such as nitrogen, phosphorus, metals, and many other major and minor nutrients, and salts.. Surface water and ground-water pollutants from organic matter and crop residue decomposition and from legumes growing on agricultural land may include nitrogen, phosphorus, and other essential nutrients found in the residue of growing crops.. All plants require nutrients for growth. In aquatic environments, nutrient availability usually limits plant growth. Nitrogen and phosphorus generally are present at background or natural levels below 0.3 and 0.05 mg/L, respectively. When these nutrients are introduced into a stream, lake, or estuary at higher rates, aquatic plant productivity may increase dramatically. This process, referred to as cultural eutrophication, may adversely affect the suitability of ...
C. atlanticus, G. forsetii, and R. biformata are marine bacteria that are thought to play important roles in the digestion of macromolecules (43-45). As with other marine bacteroidetes, these bacteria are probably enriched on surfaces such as algal cells or organic detritus particles known as marine snow, and the ability to glide over these surfaces may be important for their survival (46). R. anatipestifer is an important poultry pathogen (47), and motility may play a role in pathogenesis. P. propionicigenes, which was isolated from an anoxic rice field (48), has not been studied extensively, and the importance of gliding motility in its lifestyle is unclear.. In addition to the 22 finished genome sequences for members of the classes Flavobacteriia, Cytophagia, and Sphingobacteriia described above, draft genome sequences were available for another 27 members of these classes (see Table S3 in the supplemental material). We analyzed these and determined that all contained the 11 core gliding ...
With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , Small Business Owners , Start-ups - Business Is Chess Not Checkers. With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , Small Business Owners , Start-ups - Business Is Chess Not Checkers from Business Is Chess Not Checkers Price: USD 0 View Details about With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , […]. ...
With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , Small Business Owners , Start-ups - Business Is Chess Not Checkers. With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , Small Business Owners , Start-ups - Business Is Chess Not Checkers from Business Is Chess Not Checkers Price: USD 0 View Details about With Jay Surti & Trish Taylor , Practical Business Tips , Solopreneurs , Career Change , […]. ...
A large amount of hot filter cake (HFC) is annually generated in Iranian zinc plants. It contains 1% zinc, 16-30% manganese, 5-25% calcium and 1-4.5% cobalt. Usually, zinc is selectively leached by...
The BBCs Geeta Pandey in Mumbai meets award-winning author and cartoonist Aabid Surti who goes around fixing leaking taps in peoples homes.
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The study was conducted on eighteen postpartum silent oestrus (> 45 days postpartum) Surti buffaloes, divided into three equal groups (6 in each) to evaluate the efficacy of Norgestomet ear implant alone (Gr.-I) and in combination with PGF2α (Gr.-II). The buffaloes in Group-I and Group-II were implanted with Crestar ear implant for 9 days with 2 ml i/m injection of Crestar solution (estradiol Valerate) on the day of implant insertion. In Group-II, additionally 500 μg Cloprostenol was given i/m on one day before implant removal. The buffaloes in Group-III served as untreated silent oestrus controls and were given 5 ml Normal Saline i/m as a placebo treatment on 0 and 8th day. The estrus was induced in cent per cent of buffaloes in the Gr-I and Gr-II with mean estrus induction intervals of 2.17 0.06 and 2.03 0.05 days, respectively. These intervals were significantly (P. Key words: Estrus, Fertility, Norgestomet ear implant, PGF2α, Postpartum Silent oestrus ...
princeedwardisland.ca is the official website of the province of Prince Edward Island. Learn about Prince Edward Island and government services available to residents and business
The largest contributor to the growth of the Public Sector Pension Plan assets is normally investment income. These investments are held in the Province of Prince Edward Island Master Trust.. The Fiscal Management division of the Department of Finance provides investment management support for the Province of Prince Edward Island Master Trust.. ...
princeedwardisland.ca is the official website of the province of Prince Edward Island. Learn about Prince Edward Island and government services available to residents and business
Prince Edward Island- the key Atlantic province of Canada has opened latest draw of Prince Edward Island Provincial Nominee Program (PEI PNP) on June 20, 2019.
en] Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens, there is limited data on the presence of hormones in this type of treatment system. The objective of this study was to evaluate the ability of the CWS to reduce estrogenic and androgenic hormone concentration in dairy wastewater. This was achieved through a year long study on dairy wastewater samples obtained from a surface flow CWS. Analysis of hormonal levels was performed using a solid phase extraction (SPE) sample clean-up method, combined with reporter gene assays (RGAs) which incorporate relevant receptors capable of measuring total estrogenic or androgenic concentrations as low as 0.24 ng L(-1) and 6.9 ng L(-1) respectively. Monthly analysis showed a mean removal efficiency for estrogens of 95.2%, corresponding to an average residual concentration of 3.2 ng L(-1) 17beta-estradiol ...
The mission of the Construction Association of PEI is to foster, promote and advance the interests and efficiency of the construction industry in Prince Edward Island by acting on behalf of and for its members. ...
Best in Prince Edward Island Teaching Science Graduate Programs. Review requirements for Teaching Science degrees and accredited schools 2021 - 2022
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Prince Edward Island Provincial Nominee Program Archives - Canada Immigration and Visa Information. Canadian Immigration Services and Free Online Evaluation.
January is Alzheimers Awareness month and to commemorate this the Alzheimer Society of Prince Edward Island is teaming up with colleagues across Canada for #ConnectionsMatter.
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New blueberry research at University of Prince Edward Island is investigating whether adding probiotics to the fruit helps reduce high blood pressure.
Microbial interactions have a key role in global geochemical cycles. Although we possess significant knowledge about the general biochemical processes occurring in microbial communities, we are often unable to decipher key functions of individual microorganisms within the environment in part owing to the inability to cultivate or study them in isolation. Here, we circumvent this shortcoming through the use of single-cell genome sequencing and a novel low-input metatranscriptomics protocol to reveal the intricate metabolic capabilities and microbial interactions of an alkane-degrading methanogenic community. This methanogenic consortium oxidizes saturated hydrocarbons under anoxic conditions through a thus-far-uncharacterized biochemical process. The genome sequence of a dominant bacterial member of this community, belonging to the genus Smithella, was sequenced and served as the basis for subsequent analysis through metabolic reconstruction. Metatranscriptomic data generated from less than 500 ...
Prince Edward Head Start building on U.S. route 460, near Prospect in Prince Edward County, Va. In 1959 it was the First Rock Elementary School for African Americans. Temporary building covered with roofing paper and an outdoor privy which were seen in 1961-1962 are now gone ...
Last week also our Science Club Series resumed, with 2 events! First, on Tuesday 06 October Prof Spencer J. Greenwood (University of Prince Edward Island, Canada) gave a great talk on The Secret Life of Lobster Vets: unravelling host-pathogen-environment interactions in our favourite crustacean. Spencer is a Professor at the Department of Biomedical Sciences at the University of Prince Edward Island (Canada) and also Director-Research Scientist at the AVC Lobster Science Centre (Atlantic Veterinary College, also at the University of Prince Edward Island in Charlottetown, Canada). Spencers research focusses on crustacean biology, using molecular methods to address questions about host-pathogen interactions and aquatic species and parasitology, and is closely linked to industrial applications of new research findings. At his seminar, Spencer focussed on his research on lobsters: ...
Here,we study the mcr-containing archaeal MAGs from several hot springs, which reveal further expansion in the diversity of archaeal organisms performing methane/alkane metabolism.
ID THII_METTH Reviewed; 389 AA. AC O27720; DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1998, sequence version 1. DT 30-AUG-2017, entry version 107. DE RecName: Full=Probable tRNA sulfurtransferase {ECO:0000255,HAMAP-Rule:MF_00021}; DE EC=2.8.1.4 {ECO:0000255,HAMAP-Rule:MF_00021}; DE AltName: Full=Sulfur carrier protein ThiS sulfurtransferase {ECO:0000255,HAMAP-Rule:MF_00021}; DE AltName: Full=Thiamine biosynthesis protein ThiI {ECO:0000255,HAMAP-Rule:MF_00021}; DE AltName: Full=tRNA 4-thiouridine synthase {ECO:0000255,HAMAP-Rule:MF_00021}; GN Name=thiI {ECO:0000255,HAMAP-Rule:MF_00021}; GN OrderedLocusNames=MTH_1685; OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / OS JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium OS thermoautotrophicum). OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanothermobacter. OX NCBI_TaxID=187420; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 29096 / ...
Arcand, M.M., Levy-Booth, D.J., and Helgason, B.L. 2017. Resource legacies of organic and conventional management differentiate soil microbial carbon use. Frontiers in Microbiology, 8: 2293. https://doi.org/10.3389/fmicb.2017.02293. Arcand, M.M., Helgason, B.L. and Lemke, R.L. 2016. Microbial crop residue decomposition dynamics in organic and conventionally managed soils. Applied Soil Ecology, 107: 347-359. https://doi.org/10.1016/j.apsoil.2016.07.001. Arcand, M.M., Knight, J.D., and Farrell, R.E. 2014. Differentiating between the supply of N to wheat from above and belowground residues of preceding crops of pea and canola. Biology and Fertility of Soils, 50(4): 563-570. https://doi.org/10.1007/s00374-013-0877-4. Woodley, A., Audette, Y., Fraser, T., Arcand, M.M., Voroney, P., Knight, D., Lynch, D.H., Martin, R.C., MacRae, R. 2014. Nitrogen and phosphorus fertility management in organic field crop production. p. 59-106 in Martin, R.C. and MacRae, R. (eds) Managing Energy, Nutrients and Pests in ...
After months of delays, the town of Stratford, P.E.I., says its ready to install a wastewater treatment system next week, but the foul stench plaguing residents could linger.
These recipes have been sourced from partners, producers, family cooks, and talented chefs creating culinary masterpieces with fresh, quality Island ingredients.
We have listed disorders for which there is a general consensus among those investigating in this field and among veterinary practitioners, that the condition is significant in this breed. Where the mode of inheritance is known, this information is included on the linked disease page. The most popular breeds tend to have the most disorders listed because there is a larger number of dogs affected, and therefore more opportunity to recognize a breed predisposition to a particular disorder. As well, there is likely to be more indiscriminate breeding of these breeds, leading to a higher occurrence of inherited disorders. In less common or newer breeds, there may be no disorders listed or the list of disorders may be quite short, because it can take some time before enough dogs are affected to recognize an inherited condition ...
We have listed disorders for which there is a general consensus among those investigating in this field and among veterinary practitioners, that the condition is significant in this breed. Where the mode of inheritance is known, this information is included on the linked disease page. The most popular breeds tend to have the most disorders listed because there is a larger number of dogs affected, and therefore more opportunity to recognize a breed predisposition to a particular disorder. As well, there is likely to be more indiscriminate breeding of these breeds, leading to a higher occurrence of inherited disorders. In less common or newer breeds, there may be no disorders listed or the list of disorders may be quite short, because it can take some time before enough dogs are affected to recognize an inherited condition ...
(title:self care for the caregiver AND body:self care for the caregiver), (title:stress)^1, caregiver health, signs of fatigue, caregiver exhaustion
This is a Provincial discussion forum and a no bashing zone. This area is here for members who are looking for serious answers for serious questions or...
This Site Has Stunned Harmony Junction PE, And Its Only a Matter of Time Before Its Banned. ** You Dont Need To Use Your Actual Name on This Site! **.