TY - JOUR. T1 - Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. AU - Oh, Jin Soo. AU - Ha, Yoon. AU - An, Sung Su. AU - Khan, Momin. AU - Pennant, William A.. AU - Kim, Hyo Jin. AU - Yoon, Do Heum. AU - Lee, Minhyung. AU - Kim, Keung Nyun. PY - 2010/3/26. Y1 - 2010/3/26. N2 - Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for ...
Mesenchymal stem/stromal cells (MSCs) delivered as cell therapy to individuals with degenerative and/or inflammatory disorders can help improve organ features and resolve inflammation, as demonstrated in preclinical studies and to some extent in clinical studies. MSCs have trophic, homing/migration, and immunosuppression functions, with many benefits in therapeutics. MSC functions are thought to depend on the paracrine action of soluble factors and/or the expression of membrane-bound molecules, mostly belonging to the molecular class of adhesion molecules, chemokines, enzymes, growth factors, and interleukins. Cutting-edge studies underline bioactive exchanges, including that of ions, nucleic acids, proteins, and organelles transferred from MSCs to stressed cells, thereby improving the cells survival and function. From this aspect, MSC death modulation function appears as a decisive biological function that could carry a significant part of the therapeutic effects of MSCs. Identifying the function and
TY - JOUR. T1 - Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. AU - Singh, Pratibha. AU - Fukuda, Seiji. AU - Liu, Liqiong. AU - Chitteti, Brahmananda Reddy. AU - Pelus, Louis. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Although mesenchymal stromal cells (MSCs) have significant potential in cell-based therapies, little is known about the factors that regulate their functions. While exploring regulatory molecules potentially involved in MSC activities, we found that the endogenous multifunctional factor Survivin is essential for MSC survival, expansion, lineage commitment, and migration. Pharmacological or genetic blockade of Survivin expression in mouse and human bone marrow MSC enhances caspase 3 and 7 expression and reduces proliferation resulting in fewer MSC and clonogenic colony-forming unit-fibroblasts (CFU-F), whereas ectopic Survivin overexpression in MSC results in their expansion. Survivin is also required for the MSC proliferative responses to basic ...
ATCC ® Normal Human Adipose-Derived Mesenchymal Stem Cells, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Adipose-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC ® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Background: Developing efficient methods to isolate and identify human adipose-derived mesenchymal stem cells (hADSCs) remains to be one of the major challenges in tissue engineering.. Methods: We demonstrate here a method by isolating hADSCs from abdominal subcutaneous adipose tissue harvested during caesarian section. The hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.. Results: The yield reached around 1 x 10(6) hADSCs per gram adipose tissue. The following comprehensive identification and characterization illustrated pronounced features of mesenchymal stem cells (MSCs). The fibroblast-like hADSCs exhibited typical ultrastructure details for vigorous cell activities. Karyotype mapping showed normal human chromosome. With unique immunophenotypes they were positive for CD29, CD44, CD73, CD105 and CD166, but negative for CD31, CD34, CD45 and HLA-DR. The growth curve and cell cycle analysis revealed high capability for self-renewal and ...
OBJECTIVE To explore the response of rat bone marrow mesenchymal stem cells (MSCs and calvarial osteoblasts to mechanical strain and the consequent changes of cytoskeleton F-actin. METHODS Bone marrow MSCs and calvarial osteoblasts were isolated from SD rats and cultured in vitro. Mechanical stretch was performed on passage 3 cells at 2 000 microepsilon for 0, 2, 6 and 12 hours using four-point bending system. The response of cells and the distribution of F-actin were observed using fluorescent staining under laser scanning confocal microscope and the morphological parameters were quantified using image analysis software Laserpix. RESULTS Under mechanical stretch, the fluorescent staining decreased obviously at both MSCs and osteoblasts, and F-actin filaments were rearranged and became tenuous, thinner, and abnormally distributed. The outline of nucleus became unclear and apoptotic changes were observed at some of both cells. Cellular size decreased more significantly in MSCs than in osteoblasts.
Purpose: : To observe the differentiation potential of SD rat bone marrow mesenchymal stem cells (MSCs) which were subretinally transplanted into laser-injured retina in vivo without differentiation inducements in vitro. Methods: : SD rat MSCs were cultivated in vitro to passage 3 and were injected into the sub-retina space of laser-injured rat retina with Bromo-Deoxyuridine (BrdU) labeling to trace the origin of the repopulating cells. After 4 weeks, eyeballs were enucleated for continuous paraffin slides. The expressions of Nestin, NeuN, GFAP, Vimentin, Rhodopsin were detected by immunofluorescence. Results: : BrdU-positive MSCs were present in mutiple locations, including photoreceptor layer, the bipolar layers and the ganglion cell layer. These cells expressed Nestin, NeuN, GFAP, Vimentin, and Rhodopsin. Conclusions: : For SD rats, transplanted MSCs in injured retina can be integrated into the host retina and partially differentiate into nerve cells and photoreceptors in vivo. ...
Intranasal Administration of Mesenchymoangioblast-Derived Mesenchymal Stem Cells Abrogates Airway Fibrosis and Airway Hyperresponsiveness Associated with Chronic Allergic Airways Disease Researchers assessed the in vivo efficacy of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) on airway remodeling in a murine model of chronic allergic airways disease/asthma. Apart from epithelial thickness, all other parameters measured were significantly, although not totally, decreased by intravenous delivery of MCA-MSCs to ovalbumin-injured mice. [FASEB J] Abstract Identification and Characterization of a Rich Population of CD34+ Mesenchymal Stem/Stromal Cells in Human Parotid, Sublingual and Submandibular Glands Very little is known about the phenotype, distribution and molecular nature of mesenchymal stem/stromal cells (MSCs) in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. The authors results ...
We have used a disc-shaped self-gelling alginate hydrogel as a scaffold for in vitro chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. The comparison of monolayer cells and alginate embedded cells with or without differentiation medium allowed us to perform a detailed kinetic study of the expression of a range of genes and proteins known to be involved in chondrogenesis, using real-time polymerase chain reaction, fluorescence immunohistochemistry, and glycosaminoglycan measurement in the supernatant. mRNA encoding type II collagen (COL2), COL10, aggrecan, and SOX5, 6, and 9 were greatly elevated already at day 7, whereas COL1 and versican mRNA were gradually reduced. COL2 and aggrecan were dispersed throughout the extracellular matrix at day 21, whereas COL10 distribution was mainly intra/pericellular. COL1 seemed to be produced by only some of the cells. SOX proteins were predominantly localized in the nuclei. Then, using microarray analysis, we identified a signature
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Yu, B., Zhao, X., Yang, C., Crane, J., Xian, L., Lu, W., Wan, M. and Cao, X. (2012), Parathyroid hormone induces differentiation of mesenchymal stromal/stem cells by enhancing bone morphogenetic protein signaling. J Bone Miner Res, 27: 2001-2014. doi: 10.1002/jbmr.1663 ...
Cytotoxic T-lymphocyte-associated protein 4- (CTLA4-) modified human bone marrow-derived mesenchymal stem cells (hBMMSCs) might be promising seed cells for bone tissue engineering. However, the underlying mechanism is not clear. In the present study, we investigated whether CTLA4-modified hBMMSCs are involved in the migration of allogeneic hBMMSCs (allo-hBMMSCs) by maintaining POSTN secretion. hBMMSCs were isolated from different groups, named hBMMSCs and allo-hBMMSCs. hBMMSCs that were infected with the negative control (NC), empty adenovirus- or recombinant adenovirus-expressing CTLA4, POSTN, or CTLA4 plus the shRNA of POSTN were named NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs, respectively. They were then cocultured with PBMCs in a 1 : 5 ratio with 2.5 |i|μ|/i|g/mL phytohemagglutinin (PHA). The coculture supernatant was collected to treat allo-hBMMSCs with anti-integrin |i|α|/i|v|i|β|/i|3 IgG, or negative
The complexity of MSC secretome is hindering a definitive understanding; however, clues on the biological drivers for cardiac regeneration have been emerging and consistent evidence begins to indicate some pivotal players. VEGF is emerging as a critical paracrine factor for MSC-mediated cardioprotection. Several MSC types may also differentially release insulin-like growth factor (IGF)-1, transforming growth factor (TGF)-β2, and EGF [54-56]. AD-MSC are able to secrete numerous angiogenic, arteriogenic, chemotactic, and anti-apoptotic growth factors; for this reason their secretome has been involved in a series of novel strategies to enhance tissue restoration by increased angiogenesis [57-59]. Schenke-Layland et al. showed that AD-MSC accelerated vascularization in infarcted areas, increasing both capillary and arteriole density as a result of paracrine signaling [60]. This mechanism has been supported by other investigators who have considered adult stem cells from other sources administered ...
Osteoporosis due to estrogen deficiency is an increasing bone health issue worldwide: new strategies are being studied for regenerative medicine of bone pathologies in these patients. The most commonly used cells for tissue engineering therapy are the bone marrow mesenchymal stem cells (BMSCs), but they might be negatively affected by aging and estrogen deficiency. Besides the general advantages of adipose-derived mesenchymal stem cells (ADSCs) over BMSCs, ADSCs also seem to be less affected by aging than BMSCs, but in the literature, little is known about ADSCs in estrogen deficiency. The present study investigated the in vitro behavior of ADSCs, isolated from healthy (SHAM) and estrogen-deficient (OVX) rats. Phenotype, clonogenicity, viability, and osteogenic differentiation, at both cellular and molecular levels, were evaluated with or without osteogenic stimuli. Pro-inflammatory cytokines, growth factors, and adipogenic differentiation markers were also analyzed. There were no significant ...
BACKGROUND AIMS: Human mesenchymal stromal cells (MSC) are promising candidates for cell therapy because of their intriguing properties (high proliferation and differentiation capacity, microenvironmental function and immune modulation). However, MSC are heterogeneous and a better understanding of the heterogeneity of the cells that form the MSC cultures is critical. METHODS: Human MSC were generated in standard cultures and stained with carboxyfluorescein succinimidyl ester (CFSE) for cell division tracking. Gene expression profiling of MSC that were sorted based on functional parameters (i.e. proliferation characteristics) was utilized to characterize potential MSC subpopulations (progenitor content and differentiation capacity) and identify potential MSC subpopulation markers. RESULTS: The majority of MSC had undergone more than two cell divisions (79.7+/-2.0%) after 10 days of culture, whereas 3.5+/-0.9% of MSC had not divided. MSC were then sorted into rapidly dividing cells (RDC) and slowly/non
Introduction Stem cell therapy can promote good recovery from stroke. Several studies have demonstrated that mesenchymal stem cells (MSC) are safe and effe
The aim of the present study was to investigate the potential of bone mesenchymal stem cells (BMSCs) treated with a combination of vascular endothelial growth factor (VEGF) and bone morphogenetic protein‑6 (BMP‑6) genes for the treatment of avascular necrosis of the femoral head (ANFH). Rat BMSCs were isolated and purified using a density gradient centrifugation method. The purity and characteristics of the BMSCs were detected by cell surface antigens identification using flow cytometry. The experimental groups were administered with one of the following adeno‑associated virus (AAV) vector constructs: AAV‑green fluorescent protein (AAV‑GFP), AAV‑BMP‑6, AAV‑VEGF or AAV‑VEGF‑BMP‑6. The expression of VEGF and BMP‑6 was detected by reverse transcription‑quantitative polymerase chain reaction, western blotting and ELISA assays. The effects of VEGF and BMP‑6 on BMSCs were evaluated by angiogenic and osteogenic assays. The transfected BMSCs were combined with a biomimetic ...
Mesenchymal stem cells (MSC) may be a therapeutic option in diseases associated with severe inflammation or auto-immune diseases, due to their immunomodulatory and anti-inflammatory properties. A number of clinical trials are being conducted worldwide testing th efficacy of MSC, mainly isolated from bone marrow, for different conditions, such as Graft Versus host Disease, refractory Crohns Disease, ischemic stroke, acute myocardial infarction, type I Diabetes Mellitus, or Chronic Obstructive Pulmonary Disease. Usually, the route of administration of the cells in these studies is intravenous. Local injection of MSC for fistulizing Crohns Disease has proven efficacious. Endoscopy is a routinary technique for the evaluation of gastrointestinal and colonic conditions. The purpose of our study is to evaluate safety and efficacy of the intracolonic injection by using a colonoscope of allogeneic adipose tissue-derived MSC in patients with moderate active ulcerative colitis ...
Hepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently
TY - JOUR. T1 - In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. AU - Williams, Christopher G.. AU - Kim, Tae Kyun. AU - Taboas, Anya. AU - Malik, Athar. AU - Manson, Paul. AU - Elisseeff, Jennifer Hartt. PY - 2003/8. Y1 - 2003/8. N2 - Mesenchymal stem cells (MSCS) from skeletally mature goats were encapsulated in a photopolymerizing poly(ethylene glycol)-based hydrogel and cultured with or without transforming growth factor β1 (TGF) to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. Chondrogenic differentiation was evaluated by histological, biochemical, and RNA analyses for the expression of cartilage extracellular matrix components. The two control groups studied were MSCs cultured in monolayer and MSCs encapsulated in the hydrogel and cultured for 6 weeks in chondrogenic medium without TGF-β1 (6wk-TGF). The three experimental time points for encapsulated cells studied ...
TY - JOUR. T1 - Cellular therapeutics for heart failure. T2 - Focus on mesenchymal stem cells. AU - Pandey, Amitabh C.. AU - Lancaster, Jordan J.. AU - Harris, David T.. AU - Goldman, Steven. AU - Juneman, Elizabeth. PY - 2017. Y1 - 2017. N2 - Resulting from a various etiologies, the most notable remains ischemia; heart failure (HF) manifests as the common end pathway of many cardiovascular processes and remains among the top causes for hospitalization and a major cause of morbidity and mortality worldwide. Current pharmacologic treatment for HF utilizes pharmacologic agents to control symptoms and slow further deterioration; however, on a cellular level, in a patient with progressive disease, fibrosis and cardiac remodeling can continue leading to end-stage heart failure. Cellular therapeutics have risen as the new hope for an improvement in the treatment of HF. Mesenchymal stem cells (MSCs) have gained popularity given their propensity of promoting endogenous cellular repair of a myriad of ...
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Multipotent mesenchymal stromal cells (MSCs) have gained considerable interest because of their potential use in the treatment of a variety of diseases and injuries. Although remarkable advancements have been made in clinical studies, substantial concerns still regard the safety of MSCs. Some evidence suggests that MSCs can spontaneously generate a population of cells with tumorigenic potential. Thus, studying the molecular mechanisms that control the proliferation of MSCs may be a necessary step toward the development of strategies for safe clinical practice. Ca2+ is a second messenger that mediates a wide range of cellular responses, including the regulation of cell proliferation, but little is known about its function in MSCs. The aim of this study was to investigate the effects of targeted Ca2+ buffering on MSCs proliferation in vitro. Here, we used an adenoviral (Ad) vector encoding the Ca2+ chelator protein parvalbumin (PV) fused to a nuclear exclusion signal (NES) and the Discosoma red
This study investigates the effects of human bone marrow-derived mesenchymal stem cell (hMSC) on migration and proliferation ability of hepatocellular carcinoma (HCC) with high- and low-metastatic potential. The hMSC and transforming growth factor-β1 (TGFβ-1) gene infected hMSC were co-cultured with hepatoma cells. The ability of cells migration was assessed by Transwell assay. The ability of cells proliferation was detected using CCK-8 assay. The mice were engrafted with hMSC and TGFβ-1 gene infected hMSC, respectively, after hepatoma cells inoculation 15 days, twice a week for 6 weeks successively. The tumor inhibition rate was calculated. TGFβ-1, osteopontin (OPN), and programmed cell death protein 4 (PDCD4) genes expression of hepatoma cells were detected by quantitative real-time polymerase chain reaction (qPCR) before and after co-cultured experiments. TGFβ-1 infected hMSC or hMSC co-culture with hepatoma cells groups can significantly promote hepatoma cells proliferation (P | 0.05). The
The immunomodulatory properties of type 2 diabetic patients adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peri pheral blood mononuclear ce lls (PBMCs) proliferation ratio, protein levels of IFN- γ and IL-10, mRNA expression of COX-2 , TNF- α , TGF- β 1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days ( p , 0.001). IFN- γ level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not changed in D-ASCs co-culture. COX-2 ...
The global Human Mesenchymal Stem Cells (hMSC) market is exhaustively researched and analyzed in the report to help market players to improve their business tactics and ensure long-term success. The authors of the report have used easy-to-understand language and uncomplicated statistical images but provided thorough information and detailed data on the global Human Mesenchymal Stem Cells (hMSC) market. The report equips players with useful information and suggests result-oriented ideas to gain a competitive edge in the global Human Mesenchymal Stem Cells (hMSC) market. It shows how different players are competing in the global Human Mesenchymal Stem Cells (hMSC) market and discusses about strategies they are using to distinguish themselves from other participants.. Get the Sample of this [email protected]://www.qyresearch.com/sample-form/form/904648/global-human-mesenchymal-stem-cells-hmsc-market. The researchers have provided quantitative and qualitative analysis along with absolute dollar ...
Results In untreated mice (C) proteinuria was detectable at 17 weeks of age (3.5±2.7 mg/day) and further increased until 35 weeks of age (12.5±3.5 mg/day). At 24 weeks, proteinuria was 1.8±2.3 mg/day in C group, 0.9±0.4 mg/day in NZB/W20, 0.7±0.5 mg/day in NZB/WM20.. At 30 weeks proteinuria was 4.9±2 mg/day in untreated mice, 4.6±5 mg/day in NZB/W20, 3.2±0.2 mg/day in NZB/W24, 0.7±0.5 mg/day in NZB/WM20 and 0.8±0.4 mg/day in NZB/WM24. At 36 weeks proteinuria was 12.5±3.5 mg/day in C group, 8.5+6.1 mg/day in NZB/W20, 3.7+3.4 mg/day in NZB/W24, 9.4+5.1 mg/day in NZB/W32, 6.5±5 mg/day in NZB/WM20 (p=0.006 vs C group) and 4.5+2.5 mg/day in NZB/WM24 (P,0.0001 vs C group).. The titers of the anti-dsDNA antibody did not change between groups. No significant differences in histological kidney scores were observed between single and multiple treatments. ...
Bone marrow-derived mesenchymal stem cells (bMSCs) can differentiate into a number of different cell/tissue types, and also possess immunoregulatory functions. The present study was undertaken to elucidate the exact immunoregulatory effects of allogeneic bMSCs on T- and B-lymphocyte proliferation, activation, and function maturation of BXSB mice, which has been considered as a experimental model for human systemic lupus erythematosus (SLE). We determined that bMSCs from BALB/c mice had inhibitory effects on BXSB mice T- lymphocyte proliferation, but no inhibitory effect on their activation. In addition, they had a significant inhibitory and stimulatory effect on IL-4- and IFN-gamma-producing T cells, respectively. Also, bMSCs had inhibitory effects on the proliferation, activation, and IgG secretion of B lymphocytes. In addition, BALB/c bMSCs had an enhancing effect on CD40 expression and inhibitory effects on CD40 ligand (CD40L) ectopic hyperexpression on B cells from BXSB mice. ...
BACKGROUND: The introduction of specific BCR-ABL inhibitors in chronic myelogenous leukemia therapy has entirely mutated the prognosis of this hematologic cancer from being a fatal disorder to becoming a chronic disease. Due to the probable long lasting treatment with tyrosine-kinase inhibitors (TKIs), the knowledge of their effects on normal cells is of pivotal importance.. DESIGN AND METHODS:. We investigated the effects of dasatinib treatment on human bone marrow-derived mesenchymal stromal cells (MSCs).. RESULTS:. Our findings demonstrate, for the first time, that dasatinib induces MSCs adipocytic differentiation. Particularly, when the TKI is added to the medium inducing osteogenic differentiation, a high MSCs percentage acquires adipocytic morphology and overexpresses adipocytic specific genes, including PPARγ, CEBPα, LPL and SREBP1c. Dasatinib also inhibits the activity of alkaline phosphatase, an osteogenic marker, and remarkably reduces matrix mineralization. The increase of PPARγ is ...
Multipotent mesenchymal stromal cells (MSCs) have potential therapeutic benefit for the treatment of neurological diseases and injury. MSCs interact with and alter brain parenchymal cells by direct cell-cell communication and/or by indirect secretion of factors and thereby promote functional recover …
Basic Biology and Clinical Application of Multipotent Mesenchymal Stromal Cells: From Bench to Bedside - A special issue journal published by Hindawi
The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias. Micro-CT images and H&E staining showed more trabecular bone at the needle-implanted site in the OA group than the normal saline group. Thus, OA confers an osteoinductive effect on BMSCs via induction of osteogenic marker gene expression, such as BMP2 and Runx2 expression and subsequently elevates ALP activity and mineralization, followed
1. Kaur G, Valarmathi MT, Potts JD, Wang Q. The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus. Biomaterials. 2008;29:4074-81 2. Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR. et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41-9 3. Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Experimental biology and medicine. 2001;226:507-20 4. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD. et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-7 5. Si YL, Zhao YL, Hao HJ, Fu XB, Han WD. MSCs: Biological characteristics, clinical applications and their outstanding concerns. Ageing research reviews. 2011;10:93-103 6. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue engineering. 1998;4:415-28 7. Drost ...
Background: Bone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has been considered as a promising milestone in liver fibrosis treatment. However, low amounts of homing are a major obstacle. We aimed to investigate the role of melatonin pretreatment in BMMSC homing into experimental liver fibrosis. Methods: BMMSCs were obtained, grown, propagated and preconditioned ...
Qinge formula (QEF), prepared from an ancient Chinese recipe, was previously suggested to regulate bone metabolism and improve bone mineral density in patients with osteoporosis. To study the effects of medicated serum containing QEF on the in vitro differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) isolated from the proximal femurs of postmenopausal osteoporosis (PMOP) mice. Using an established mouse model of PMOP, mononuclear cells were isolated from the bone marrow present in the proximal femurs and cultured. PMOP mice were also randomly divided into four groups: the untreated group (Group A) and the groups treated with respectively low (Group B), medium (Group C), and high (Group D) concentrations of QEF. Serum was isolated from each and used to treat the cultured BMSCs in conjunction with recombinant human bone morphogenetic protein-2 (rhBMP-2). Cell morphology, proliferation rates, intracellular alkaline phosphatase (ALP) activity, and transforming growth factor-beta 1 (TGF
Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene
Adipose-derived mesenchymal stem cell protects kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory ...
Multipotent mesenchymal stem/stromal cells (MSC) including adipose-derived stromal cells (ADSC) have been successfully applied for cardiovascular diseases treatment. Their regenerative potential is considered due to the multipotency, paracrine activity and immunologic privilege. However, therapeutic efficacy of autologous MSC for myocardial ischemia therapy is modest. We analyzed if ADSC properties are attenuated in patients with chronic diseases such as coronary artery disease (CAD) and diabetes mellitus type 2 (T2DM). ADSC were isolated from subcutaneous fat tissue of patients without established cardiovascular diseases and metabolic disorders (control group, n = 19), patients with CAD only (n = 32) and patients with CAD and T2DM (n = 28). ADSC phenotype (flow cytometry) was CD90+/CD73+/CD105+/CD45−/CD31− and they were capable of adipogenic and osteogenic differentiation. ADSC morphology and immunophenotype were similar for all patients, but ADSC from patients with CAD and T2DM had higher
PromoCell announces services for the large-scale industrial lots of human mesenchymal stem cells (hMSCs). As a leading manufacturer of stem cell research products, PromoCell has over a decade of experience in the production of mesenchymal stem cells derived from bone marrow, umbilical cord and adipose tissue that ensure the highest level of standardization in accordance with ISCT* guidelines. With the growing demand for highly standardized MSC populations in cell-based therapy applications, PromoCell has now introduced their large-scale production service for mesenchymal stem cells derived from bone marrow and adipose tissue. The cells are offered as industrial lots that guarantee the access to a huge number of cells from the same lot with same specifications and quality.. Mesenchymal stem cells, also termed mesenchymal stromal cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self-renewal. MSCs are capable of differentiating into ...
Extracellular Matrix Protein Laminin Enhances Mesenchymal Stem Cell (MSC) Paracrine Function through αvβ3/CD61 Integrin to Reduce Cardiomyocyte Apoptosis Researchers found that placenta-derived multipotent cells (PDMCs) significantly reduced cardiomyocyte apoptosis and reactive oxygen species production through the paracrine factors GRO-α, HGF and IL-8. The enhancement of PDMC paracrine function by laminin was mediated through αvβ3 integrin, with involvement of the signaling pathways of JNK, for GRO-α and IL-8 secretion, and PI3K/AKT, for HGF secretion. [J Cell Mol Med] Full Article Transplantation of Human Bone Marrow Mesenchymal Stromal Cells Reduces Liver Fibrosis More Effectively than Whartons Jelly Mesenchymal Stromal Cells The authors compared the relative therapeutic potential of pooled, adult human bone marrow (BM)- and neonatal Whartons jelly-derived mesenchymal stromal cells (MSCs) to treat CCl4-induced liver fibrosis in rats. BM-MSCs treatment showed a marked reduction in ...
Mesenchymal stem cells from human bone marrow (hMSC) have the potential to differentiate into hepatocyte-like cells in vitro and continue to maintain important hepatocyte functions in vivo after transplantation into host mouse livers. Here, hMSC were differentiated into hepatocyte-like cells in vitro (hMSC-HC) and transplanted into livers of immunodeficient Pfp/Rag2−/− mice treated with a sublethal dose of acetaminophen (APAP) to induce acute liver injury. APAP induced a time- and dose-dependent damage of perivenous areas of the liver lobule. Serum levels of aspartate aminotransferase (AST) increased to similar levels irrespective of hMSC-HC transplantation. Yet, hMSC-HC resided in the damaged perivenous areas of the liver lobules short-term preventing apoptosis and thus progress of organ destruction. Disturbance of metabolic protein expression was lower in the livers receiving hMSC-HC. Seven weeks after APAP treatment, hepatic injury had completely recovered in groups both with and without hMSC
INTRODUCTION: The goal of this study is to analyze the potential immunosuppressive properties of mesenchymal stem cells (MSC) on T cell proliferation and in collagen-induced arthritis (CIA). An additional aim is to investigate the role of interferon-gamma (IFN-gamma) in these processes. METHODS: MSC were isolated from bone marrow of DBA/1 wild type and IFN-gamma receptor knock-out (IFN-gammaR KO) mice and expanded in vitro. Proliferation of anti-CD3-stimulated CD4+ T cells in the presence or absence of MSC was evaluated by thymidine incorporation. CIA was induced in DBA/1 mice and animals were treated with MSC by intravenous or intraperitoneal injections of wild type or IFN-gammaR KO MSC. RESULTS: Purity of enriched MSC cultures was evaluated by flow cytometry and their ability to differentiate into osteoblasts and adipocytes. In vitro, wild type MSC dose-dependently suppressed anti-CD3-induced T cell proliferation whereas IFN-gammaR KO MSC had a significantly lower inhibitory potential. A role ...
Differences between stromal populations derived from various tissues are becoming more evident, and represent a source of heterogeneity within the mesenchymal phenotype. All stromal cells are essentially able to differentiate into the three mesenchymal fates in vitro, although they do not follow the same molecular paths. The cells seem to keep expression "memory" of source-specific genes that travel along during the differentiation process [41]. Recent transcriptomic studies developed in murine endothelial cells from a plethora of tissues have also manifested the tissue-specificity of their molecular signatures, heterogeneity that is explained by their function in maintenance and regeneration of the different microenvironments [42].. Using our in-house dataset to contrast tissue populations against each other, we were able to describe a repertoire of tissue-specific genes from four tissue stromal populations: bone marrow, placental and adipose tissue MSCs also in addition to dermal fibroblasts. ...
There is great interest in mesenchymal stromal cells (MSCs) for cell-therapy and tissue engineering approaches. MSCs are currently expanded in vitro in the presence of fetal calf serum (FCS); however, FCS raises concerns when used in clinical grade preparations. The aim of this study was to evaluate whether MSCs expanded in medium supplemented with platelet-lysate (PL), already shown to promote MSC growth, are endowed with biological properties appropriate for cell-therapy approaches. We confirm previously published data showing that MSCs expanded in either FCS or PL display comparable morphology, phenotype, and differentiation capacity, while PL-MSCs were superior in terms of clonogenic efficiency and proliferative capacity. We further extended these data by investigating the immune-regulatory effect of MSCs on the alloantigen-specific immune response in mixed lymphocyte culture (MLC). We found that MSCs-PL are comparable to MSCs-FCS in their capacity to: (i) decrease alloantigen-induced ...
Wnt11, a member of the evolutionarily conserved Wnt family of glycoproteins, plays important roles in cellular migration, proliferation, and differentiation during embryogenesis. However, the impact of Wnt11 signaling in cardiac repair after an acute myocardial infarction (MI) remains largely unknown. Based on the ability of Wnt11 to induce cardiomyogenic differentiation in adult cells, we hypothesized that pretreatment of bone marrow mesenchymal stem cells (MSCs) with Wnt11 would improve the outcomes of infarct repair. In vitro, Wnt11 pretreatment upregulated the expression of antiapoptotic and angiogenic molecules in MSCs. Wnt11 also increased the expression of Nkx2.5 (Figure), a marker of cardiac commitment. To test the efficacy of Wnt11 pretreatment on MSC-induced cardiac repair in vivo, MSCs were cultured in presence of Wnt11-secreting 293 cells (on an insert) for 5 days and transplanted intramyocardially after a reperfused MI. Age-matched male wild-type (C57BL/6J) mice underwent a 30-min ...
This paper represents a study on the effect of electrical pulses on adult stem cells, especially on proliferation control and also as a method of deliverin
Through the injection of Hearticellgram-AMI into acute myocardial infarction patients who are the primary targets of the drug, long term efficacy in the improvement of the left ventricle ejection fraction upon the first cell treatment is to be evaluated and compared with the current existing treatments (contemporary drug treatment).This study will also compare the efficacy and safety of single dose of hearticellgram-AMI to a two dose regimen of hearticellgram-AMI ...
Purpose : Macrophages (Mq) are a key player and regulator of inflammation and repair. Mesenchymal stromal/stem cells (MSCs) are capable of modulating the immune system through interaction with a wide range of immune cells. We evaluated the immunophenotype of Mq after co-culture with either corneal-limbal derived MSCs (CL-MSC) or bone marrow-derived MSCs (BM-MSC). Methods : BM-MSC were isolated from healthy human donors in a GMP facility. CL-MSCs were extracted from cadaver human corneas. MSCs at passage 4 to 6 from at least 5 donors were used for all experiments. CD14+ monocytes were isolated from peripheral blood mononuclear cells and differentiated into Mqs with IMDM + 10% serum for 7 days. They were cocultured with MSCs in a 0.22 µm pore size transwell for 3 days. Cell surface and intracellular antigen expression of Mqs was investigated using fluorescence-activated cell sorting (Fortessa, BD). Results : Median fluorescence intensity (MFI) of CD163 increased to 686.7 ± 207.4 and 662.1 ± ...
Sigma-Aldrich offers abstracts and full-text articles by [Xue-Qing Wang, Yong Shao, Chong-Yi Ma, Wei Chen, Lu Sun, Wei Liu, Dong-Yang Zhang, Bi-Cheng Fu, Kai-Yu Liu, Zhi-Bo Jia, Bao-Dong Xie, Shu-Lin Jiang, Ren-Ke Li, Hai Tian].
Jan A. Nolta, Ph.D., is the director of the Stem Cell Program at UC Davis School of Medicine and directs the universitys Institute for Regenerative Cures. She also serves as scientific director of the large UC Davis Good Manufacturing Practice facility and the California Umbilical Cord Blood Collection Program. In 2013, she was named one of the "Top 50 Global Stem Cell Influencers.". The UC Davis Stem Cell Program has more than 150 faculty members collaborating to work toward regenerative medicine-related cures for a spectrum of diseases and injuries. The current research in Noltas laboratory is focused on developing therapies that will use gene-modified bone marrow-derived mesenchymal stem/stromal cells to deliver factors for treating Huntingtons disease and vascular disorders. The group she oversees at UC Davis Shared Translational Laboratory is helping university teams develop numerous clinical trials for gene and cell therapy, with several adult stem cell therapies already in the clinic ...