Whether respiratory epithelial cells regulate the final transit of extravasated neutrophils into the inflamed airspace or are a passive barrier is poorly understood. Alveolar epithelial type 1 (AT1) cells, best known for solute transport and gas exchange, have few established immune roles. Epithelial membrane protein 2 (EMP2), a tetraspan protein that promotes recruitment of integrins to lipid rafts, is highly expressed in AT1 cells but has no known function in lung biology. Here, we show that Emp2-/- mice exhibit reduced neutrophil influx into the airspace after a wide range of inhaled exposures. During bacterial pneumonia, Emp2-/- mice had attenuated neutrophilic lung injury and improved survival. Bone marrow chimeras, intravital neutrophil labeling, and in vitro assays suggested that defective transepithelial migration of neutrophils into the alveolar lumen occurs in Emp2-/- lungs. Emp2-/- AT1 cells had dysregulated surface display of multiple adhesion molecules, associated with reduced raft ...
Shop Antigenic integral membrane glycoprotein ELISA Kit, Recombinant Protein and Antigenic integral membrane glycoprotein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Podoplanin is a type I integral membrane glycoprotein with diverse tissue distribution, expressed at high levels in lung, placenta, and skeletal muscle. In humans, it is encoded by the PDPN gene. The exact function of podoplanin is unknown, but it is thought to be involved in cell migration, motility, and adhesion. Podoplanin is required for normal lung cell proliferation and alveolus formation at birth, and has been proposed as a marker for lung injury. Podoplanin is also known as lung type I cell membrane associated glycoprotein, glycoprotein 36 (GP36), GP38, GP40, T1-alpha, T1A, T1A-2, OTS8, AGGRUS, HT1A-1, and PA2.26.. ...
There are different subpopulations of Th17 cells that can express either a regulatory or inflammatory gene signature depending on their exposure to pathogens, cytokines, and other environmental factors such as sodium chloride. Podoplanin is a type I transmembrane glycoprotein proposed to regulate T cells in mouse models of Th17 inflammation. Here, we demonstrate that induction of podoplanin expression drives a more immunosuppressive regulatory T cell program with loss of IL-17 secretion in human CD4+ cells. Ligation of PDPN by its ligand CLEC-2 on CD4+ cells induced under Th17 conditions ameliorates the Th17 inflammatory response. Moreover, under proinflammatory, high NaCl conditions, PDPN expression is reduced via an Sgk1-mediated pathway. Finally, IL-17 secretion is restored with shRNA gene silencing of PDPN. Clinically, CD3+PDPN+ T cells are devoid of IL-17 in skin biopsies from patients with candidiasis, a Th17-driven skin disease. Thus, PDPN expression identifies a nonpathogenic Th17 ...
The history of cell culture dates back to 1907 when Ross Harrison discovered that neuronal cells could be cultured in vitro. Two types of cells used within cell culture include primary and established cell lines. Among established cells HEK 293 and 293T cells are among the most widely used in the technique of cell culture. Lymphatic endothelial cells (LECs) are often represented as primary cells, which have been isolated from tissues. Previous studies have characterized 293 and 293T cells as neuronal in origin although phenotypic analyses have not been reported. Similarly, studies dealing with the binding characteristics of HIV have revealed the expression of podoplanin, a typical LEC marker, by 293T cells. Additionally, our lab has observed the expression of podoplanin by 293 cells through flow cytometry analysis. These observations of podoplanin expression suggest that the phenotype of 293 and 293T cells needs to be further explored and that they might resemble LECs. This study outlines the ...
Complete information for EMP2 gene (Protein Coding), Epithelial Membrane Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
CD277 (known as BT3.1, BTN3A1, BTF5) is a 56 kD type I transmembrane glycoprotein. It is one of the three (BT3.1, BT3.2, and BT3.3) BT3 subfamily members. BT3 belongs to butyrophilin (BT)/B7 family and Ig superfamily. BT3.1 is expressed by T cells, B cells, monocytes, NK cells, dendritic cells, subset of stem cells, endothelial cells and some tumor cells. It has been proposed to regulate T cell activation through interaction with its counter-receptor (BT3.1-R ...
Shop P-selectin glycoprotein ligand ELISA Kit, Recombinant Protein and P-selectin glycoprotein ligand Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Liang, W and Cohen, E P., Detection of thymus leukemia antigens on the surface membranes of murine leukemia cells resistant to thymus leukemia anti- bodies and guinea pig complement. (1977). Subject Strain Bibliography 1977. 2311 ...
EMP-2 belongs to the peripheral myelin protein 22 (PMP22) family (together with EMP-1 and EMP-3), which are tetraspan transmembrane proteins.. It plays an important role in blastocyst implantation with the uterine endometrium. It plays a role in female reproductive cancers. ...
View our 16 BTN1A1/Butyrophilin products for your research including BTN1A1/Butyrophilin Primary Antibodies, Proteins and Enzymes, and cDNA Clones.
To determine the primary structure of CD13, a 150-kD cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells, we isolated the complete sequences encoding the polypeptide in overlapping complementary DNA (cDNA) clones. The authenticity of our cDNA clones …
Human CellExp Osteoactivin / GPNMB, human recombinant protein, GPNMB, HGFIN, NMB, Osteoactivin validated in (PBV11132r-10), Abgent
Myc-DDK-tagged ORF clone of Homo sapiens butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 1 as transfection-ready DNA - 10 µg - OriGene - cdna clones
Hourani, B T.; Chace, N M.; and Pincus, J H., Isolation of membrane glycoproteins from l1210 cells with lectin receptor activities. Abstr. (1973). Subject Strain Bibliography 1973. 653 ...
package Organism; use strict; use warnings; use feature state; sub count { return scalar keys %{ shift-,{Cells} }; } # Input a list of [ x, y ] coords sub insert_cells { my $cells = shift-,{Cells}; for my $r (@_) { $cells-,{ pack ii, @{$r} } = undef } } # Return sorted list of cells in the Organism. # Used for verification and testing the state of the organism. sub get_live_cells { sort { $a-,[0] ,=, $b-,[0] ,, $a-,[1] ,=, $b-,[1] } map { [ unpack ii, $_ ] } keys %{ shift-,{Cells} }; } # Return the list of dead cells surrounding a cell sub get_dead_cells { my ( $cells, $x0, $y0 ) = ( shift-,{Cells}, @_ ); my ( $x1, $x2, $y1, $y2 ) = ( $x0 - 1, $x0 + 1, $y0 - 1, $y0 + 1 ); state ( $k1, $k2, $k3, $k4, $k5, $k6, $k7, $k8 ); ( ( $k1 = pack ii, $x1, $y1 ) x !( 0 + exists $cells-,{ $k1 } ), ( $k2 = pack ii, $x1, $y0 ) x !( 0 + exists $cells-,{ $k2 } ), ( $k3 = pack ii, $x1, $y2 ) x !( 0 + exists $cells-,{ $k3 } ), ( $k4 = pack ii, $x0, $y1 ) x !( 0 + exists $cells-,{ $k4 } ), ( $k5 = ...
write down a functional definition of a eukaryotic gene. please comprise all parts of a eukaryotic gene and what the, Hire Biology Expert, Ask Academics Expert, Assignment Help, Homework Help, Textbooks Solutions
Podoplanin (Pdpn) is a type 1 transmembrane mucin-type O-glycoprotein [1, 2]. It consists of 172 amino acids in mice and 163 amino acids in humans. It is expres...
Podoplanin / gp36兔多克隆抗体(ab10274)可与人样本反应并经WB, IP, ICC/IF实验严格验证,被4篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
购买我们的重组人TREM1蛋白。Ab82984为蛋白片段,在大肠杆菌中生产并经过Western blot实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。中国80%以上现货。
In this study, we show that posttranslational folding of Vesicular Stomatitis virus G protein subunits can involve noncovalent, multimeric complexes as transient intermediates. The complexes are heterogeneous in size (4-21S20,W), contain several G glycopolypeptides, and are associated with BiP/GRP78. The newly synthesized, partially intrachain disulfide-bonded G proteins enter these complexes immediately after chain termination, and are released 1-4 min later as fully oxidized, trimerization-competent monomers. These monomers are properly folded, judging by their binding of conformation-specific mAbs. When the G protein is translated in the presence of DTT, it remains reduced, largely unfolded and aggregated in the ER, but it can fold successfully when the DTT is removed. In this case, contrary to normal folding, the aggregates become transiently disulfide cross-linked. We also demonstrated that the fidelity of the folding process is dependent on metabolic energy. Finally, we established that ...
The eye is a privileged site that cannot tolerate destructive inflammatory responses. Inflammatory cells entering the anterior chamber of the eye in response to viral infection underwent apoptosis that was dependent on Fas (CD95)-Fas ligand (FasL) and produced no tissue damage. In contrast, viral infection in gld mice, which lack functional FasL, resulted in an inflammation and invasion of ocular tissue without apoptosis. Fas-positive but not Fas-negative tumor cells were killed by apoptosis when placed within isolated anterior segments of the eyes of normal but not FasL-negative mice. FasL messenger RNA and protein were detectable in the eye. Thus, Fas-FasL interactions appear to be an important mechanism for the maintenance of immune privilege. ...
Anti-P-selectin Glycoprotein Ligand-1 (PSGL-1) Monoclonal Antibody, Unconjugated, Clone KPL-1 from CHEMICON,Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest rolling of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has bee,biological,biology supply,biology supplies,biology product
The cell surface receptor Fas (FasR, Apo-1, CD95) and its ligand (FasL) are mediators of apoptosis that have been shown to be implicated in the peripheral deletion of autoimmune cells, activation-induced T cell death, and one of the two major cytolytic pathways mediated by CD8+ cytolytic T cells. To gain further understanding of the Fas system., we have analyzed Fas and FasL expression during mouse development and in adult tissues. In developing mouse embryos, from 16.5 d onwards, Fas mRNA is detectable in distinct cell types of the developing sinus, thymus, lung, and liver, whereas FasL expression is restricted to submaxillary gland epithelial cells and the developing nervous system. Significant Fas and FasL expression were observed in several nonlymphoid cell types during embryogenesis, and generally Fas and FasL expression were not localized to characteristic sites of programmed cell death. In the adult mouse, RNase protection analysis revealed very wide expression of both Fas and FasL. ...
The cell surface receptor Fas (FasR, Apo-1, CD95) and its ligand (FasL) are mediators of apoptosis that have been shown to be implicated in the peripheral deletion of autoimmune cells, activation-induced T cell death, and one of the two major cytolytic pathways mediated by CD8+ cytolytic T cells. To gain further understanding of the Fas system., we have analyzed Fas and FasL expression during mouse development and in adult tissues. In developing mouse embryos, from 16.5 d onwards, Fas mRNA is detectable in distinct cell types of the developing sinus, thymus, lung, and liver, whereas FasL expression is restricted to submaxillary gland epithelial cells and the developing nervous system. Significant Fas and FasL expression were observed in several nonlymphoid cell types during embryogenesis, and generally Fas and FasL expression were not localized to characteristic sites of programmed cell death. In the adult mouse, RNase protection analysis revealed very wide expression of both Fas and FasL. ...
Wael Haddad, Cristine J. Cooper, Zheng Zhang, Jeffrey B. Brown, Yuechun Zhu, Andrew Issekutz, Ivan Fuss, Hae-ock Lee, Geoffrey S. Kansas, Terrence A. Barrett ...
Mokkapati, Sharada (2007) Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin. [Thesis Abstract]. ...
The 65-kDa protein (p65) was previously identified as a phosphorylated protein in activated macrophages, and has turned out to be a member of a plastin protein family characterized by a series of Ca,SUP,2+,/SUP,-, calmodulin-, and β-actin-binding domains. In mice, two isoforms, p65/L-plastin and T-plastin, have so far been identified; p65/L-plastin is expressed in hemopoietic cells and cancer cells, and T-plastin in solid tissue cells. We generated monoclonal antibodies to p65/L-plastin, examined the isoform-specificity by using recombinant (r) T-plastin, and found that the antibodies were specific for rp65/L-plastin, whereas immune sera to rp65/L-plastin showed cross-reactions to rT-plastin. One of the antibodies, p65-7B5, was demonstrated to react to native p65/L-plastin by Western blot, flow cytometric, and immunohistochemical analysis. Furthermore, p65-7B5 has made it possible to detect p65/L-plastin-expressing cells in tissues where T-plastin is abundantly expressed. These reagents and ...
Modulation of FasL expression in HT29 and RHT29 cells lines.FasL expression was detected in HT29 and RHT29 cell lines treated or not with oxaliplatin 10 µM by
subject:1450510 title:Assignment of O-Glycan Attachment Sites to the Hinge-like Regions of Human Lysosomal Membrane Glycoproteins Lamp-1 and Lamp-2 author:Carlsson، نويسنده , , S.R. and Lycksell، نويسنده , , P.O. and Fukuda، نويسنده , , M.، نويسنده ,
Fratricide has been defined as firing on your own forces, when mistaking them for enemy forces, which results in injury or death. Rates of fratricide incidence
Please register to see prices including your discount. Registration may take up to 24h. For guest orders the standard discount of your institution will apply and discounts are visible on the order confirmation.. ...
|strong|Mouse anti Human CD68 antibody, clone Ki-M7|/strong| recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expresse…
CALHM3 Peptides and Proteins available through Novus Biologicals. Browse our CALHM3 Peptides and Proteins all backed by our Guarantee+.
Berilyum, krom, germanyum, vanadyum, galliyum, hafniyum (selti- yum), indiyum, niobyum (kolombiyum), renyum ve talyum ve bu metallerden e ya (d k nt ve hurdalar dahil ...
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely replaced by the G glycoprotein of vesicular stomatitis virus. Such vectors can be concentrated by ultracentrifugation to titers , 10(9) colony-forming units/ml and can infect cells, such as hamster and fish cell lines, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein. The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo. The availability of these pseudotyped vectors will also facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system, through the efficient ...
Juvenile Neuronal Ceroid Lipofuscinosis (JNCL) is a fatal disorder. Currently treatment is symptomatic. Thus, there is a real need to intervene and slow the progression of this disease. Preliminary data on genetic knock-down of the ability to mount an immune response in cln3-knockout mice is supportive of a strategy for treating JNCL with an immuno-suppressive agent. Many drugs with the ability to suppress the immune system are steroidal and deemed unsuitable for long-term administration to children. Mycophenolate mofetil (CellCept) is used as an immunosuppressive agent in allogenic transplants in pediatric patients and is therefore approved by the Food and Drug Administration (FDA) for pediatric use.. The study design is a double-blind, randomized, 22-week cross-over study of mycophenolate mofetil vs. placebo. After a 4-week washout period, subjects will undergo blinded crossover from active study drug to placebo or from placebo to active study drug.. Subjects and caregivers will be evaluated ...
Juvenile Neuronal Ceroid Lipofuscinosis (JNCL) is a fatal disorder. Currently treatment is symptomatic. Thus, there is a real need to intervene and slow the progression of this disease. Preliminary data on genetic knock-down of the ability to mount an immune response in cln3-knockout mice is supportive of a strategy for treating JNCL with an immuno-suppressive agent. Many drugs with the ability to suppress the immune system are steroidal and deemed unsuitable for long-term administration to children. Mycophenolate mofetil (CellCept) is used as an immunosuppressive agent in allogenic transplants in pediatric patients and is therefore approved by the Food and Drug Administration (FDA) for pediatric use.. The study design is a double-blind, randomized, 22-week cross-over study of mycophenolate mofetil vs. placebo. After a 4-week washout period, subjects will undergo blinded crossover from active study drug to placebo or from placebo to active study drug.. Subjects and caregivers will be evaluated ...
TY - JOUR. T1 - Vesicular stomatitis virus glycoprotein contains a dominant cytoplasmic basolateral sorting signal critically dependent upon a tyrosine. AU - Thomas, DNette C.. AU - Brewer, Colleen B.. AU - Roth, Michael G.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - To investigate the contribution of the cytoplasmic domain of the vesicular stomatitis virus G glycoprotein to its basolateral expression in polarized epithelial cells, chimeric proteins containing the external and transmembrane domains of an apically targeted protein, the influenza virus hemagglutinin (HA), and either the G cytoplasmic domain or an unrelated cytoplasmic sequence, were introduced into Madin-Darby canine kidney (MDCK) cells. Addition of the cytoplasmic tail of G to a truncated HA resulted in delivery of greater than 95% of the chimeric protein to the basolateral cell surface, indicating that the G cytoplasmic domain contains a dominant basolateral sorting signal. A similar chimera, containing the cytoplasmic tail of herpes ...
Clinical trial for Pleural Effusion , Use of the Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) in the Diagnosis of Pleural Effusion
NEW YORK, NY and CLEVELAND, OH--(Marketwired - March 02, 2016) - AB0-201 (AAV CLN3) demonstrates promising in vivo efficacy in preclinical models IND enabling toxicology to commence in 2016 Abeona Therapeutics,…
The report on the Global T Cell Surface Glycoprotein CD3 Epsilon Chain market offers complete data on the T Cell Surface Glycoprotein CD3 Epsilon Chain market. Components, for example, main players, analysis, size, situation of the business, SWOT analysis, and best patterns in the market are included in the report. In addition to this, the report sports numbers, tables, and charts that offer a clear viewpoint of the T Cell Surface Glycoprotein CD3 Epsilon Chain market. The top contenders Amgen Inc, Celgene Corp, F. Hoffmann-La Roche Ltd, GlaxoSmithKline Plc, MacroGenics Inc, Meridigen Biotech Co Ltd, Numab Innovation AG, SYNIMMUNE GmbH, Tiziana Life Sciences Plc of the global T Cell Surface Glycoprotein CD3 Epsilon Chain market are further covered in the report .. Access to the sample pages of the report at: http://www.extentresearch.com/request-for-sample.html?repid=20413. The report also segments the global T Cell Surface Glycoprotein CD3 Epsilon Chain market based on product mode and ...
The aim of our study was to investigate the predictive value of the biomarkers interleukin 6 (IL-6), interleukin 10 (IL-10) and lipopolysaccharide-binding protein (LBP) compared with clinical CRB and CRB-65 severity scores in patients with community-acquired pneumonia (CAP). Samples and data were obtained from patients enrolled into the German CAPNETZ study group. Samples (blood, sputum and urine) were collected within 24 h of first presentation and inclusion in the CAPNETZ study, and CRB and CRB-65 scores were determined for all patients at the time of enrollment. The combined end point representative of a severe course of CAP was defined as mechanical ventilation, intensive care unit treatment and/or death within 30 days. Overall, a total of 1,000 patients were enrolled in the study. A severe course of CAP was observed in 105 (10.5%) patients. The highest IL-6, IL-10 and LBP concentrations were found in patients with CRB-65 scores of 3-4 or CRB scores of 2-3. IL-6 and LBP levels on enrollment in the
The cDNA sequence of mouse LAMP 2: evidence for two classes of lysosomal membrane glycoproteins is an eagle-i resource of type Journal article at eagle-i Network Shared Resource Repository.
Blocking the Fas/FasL interaction in vivo rescues isotype-switched B cells in μMT mice. A neutralizing recombinant FasIg protein was purified and engineered in
A stable cell line expressing a complementary DNA clone encoding the vesicular stomatitis virus glycoprotein fused and formed polykaryons at pH 5.5. The formation of polykaryons was dependent on the presence of glycoprotein anchored at the cell surface and could be prevented by incubation of cells with a monoclonal antibody to the glycoprotein. Fusion occurred at a pH 0.5 unit lower than that observed for cells infected with vesicular stomatitis virus. ...
Two murine lysosome-associated membrane proteins, LAMP-1 of 105,000-115,000 D and LAMP-2 of 100,000-110,000 D, have been identified by monoclonal antibodies that bind specifically to lysosomal membranes. Both glycoproteins were distinguished as integral membrane components solubilized by detergent solutions but not by various chaotropic agents. The lysosome localization was demonstrated by indirect immunofluorescent staining, co-localization of the antigen to sites of acridine orange uptake, and immunoelectron microscopy. Antibody binding was predominantly located at the limiting lysosomal membrane, distinctly separated from colloidal gold-labeled alpha-2-macroglobulin accumulated in the lumen during prolonged incubation. LAMP-1 and LAMP-2 also appeared to be present in low concentrations on Golgi trans-elements but were not detected in receptosomes marked by the presence of newly endocytosed alpha-2-macroglobulin, or in other cellular structures. LAMP-1 and LAMP-2 were distinguished as ...
Polyclonal and monoclonal antibodies were raised against a synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of vesicular stomatitis virus glycoprotein (VSV-G). The polyclonal antibodies (alpha P4) reacted with epitopes distributed along the 15-residue peptide, whereas the m …
TY - JOUR. T1 - A knock-in reporter model of Batten disease. AU - Eliason, Steven L.. AU - Stein, Colleen S.. AU - Mao, Qinwen. AU - Tecedor, Luis. AU - Ding, Song Lin. AU - Gaines, D. Meredith. AU - Davidson, Beverly L.. PY - 2007/9/12. Y1 - 2007/9/12. N2 - Juvenile neuronal ceroid lipofuscinosis is a severe inherited neurodegenerative disease resulting from mutations in CLN3 (ceroid- lipofuscinosis, neuronal 3, juvenile). CLN3 function, and where and when it is expressed during development, is not known. In this study, we generated a knock-in reporter mouse to elucidate CLN3 expression during embryogenesis and after birth and to correlate expression and behavior in a CLN3-deficient mouse. In embryonic brain, expression appeared in the cortical plate. In postnatal brain, expression was prominent in the cortex, subiculum, parasubiculum, granule neurons of the dentate gyrus, and some brainstem nuclei. In adult brain, reporter gene expression waned in most areas but remained in vascular endothelia ...
Definition of Counter-attacks in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Counter-attacks? Meaning of Counter-attacks as a legal term. What does Counter-attacks mean in law?
In contrast to apoptosis induced by some anticancer drugs as well as other stimuli, such as UV irradiation, anti-CD3 stimulation, and mitogen treatment (54, 55, 56, 57, 58, 59), Tet induced T cell apoptosis through a mechanism independent of Fas/FasL interaction. This conclusion was supported by several observations. First, the expression of Fas/FasL mRNA was not enhanced by Tet treatment (our unpublished observations). Second, Tet did not increase Fas/FasL expression on the T cell surface (Fig. 5⇑). Third, both Fas-sensitive and Fas-resistant T cells showed comparable susceptibility to Tet-induced apoptosis (Fig. 6⇑). Lastly, Tet-induced activation of caspase-3 appeared to have similar kinetics and intensity in Fas-sensitive and Fas-resistant T cells (Fig. 8⇑). This observation suggests that Tet can activate the T cell death machinery without the participation of cell surface Fas/FasL molecules. Treatment with Tet might be useful in autoimmune disorders caused by mutations in Fas or FasL ...
Interferons (IFNs) are well described to be rapidly induced upon pathogen-associated pattern recognition. After binding to their respective IFN receptors and activation of the cellular JAK/signal transducer and activator of transcription signaling cascade, they stimulate the transcription of a plethora of IFN-stimulated genes (ISGs) in infected as well as bystander cells such as the non-infected epithelium and cells of the immune system. ISGs may directly act on the invading pathogen or can either positively or negatively regulate the innate and adaptive immune response. However, IFNs and ISGs do not only play a key role in the limitation of pathogen spread but have also been recently found to provoke an unbalanced, overshooting inflammatory response causing tissue injury and hampering repair processes. A prominent regulator of disease outcome, especially in-but not limited to-respiratory viral infection, is the IFN-dependent mediator TRAIL (TNF-related apoptosis-inducing ligand) produced by several
Preclinical data suggests both the delivery and the therapy are working, so far.. This is the first demonstration of a systemic delivery route to restore CLN3 in vivo, using the specially-engineered virus, concludes a study published by Dr. Kielian and her collaborators in a recent edition of The Journal of Neuroscience.. CLN3 is the gene that shows a mutation in juvenile Batten disease. Also known as juvenile neuronal ceroid lipofuscinosis, the disease is an inherited, autosomal recessive, fatal neurodegenerative disorder.. Juvenile Batten disease occurs in children, and is often first noticeable between ages 5 and 10. The first symptom is vision loss, followed by seizures, cognitive loss and motor decline. Finally, premature death. There are no therapeutics or cure.. Its a rare but devastating disease.. But Dr. Kielians research shows, in an animal model, that the virus is an effective delivery system. It spreads through the body, even crossing the blood-brain barrier, from a single ...
The apoptosis-inducing Fas ligand (FasL) is a type II transmembrane protein that is involved in the downregulation of immune reactions by activation-induced cell death (AICD) as well as in T cell-mediated cytotoxicity. Proteolytic cleavage leads to the generation of membrane-bound N-terminal fragments and a soluble FasL (sFasL) ectodomain. sFasL can be detected in the serum of patients with dysregulated inflammatory diseases and is discussed to affect Fas-FasL-mediated apoptosis. Using pharmacological approaches in 293T cells, in vitro cleavage assays as well as loss and gain of function studies in murine embryonic fibroblasts (MEFs), we demonstrate that the disintegrin and metalloprotease ADAM10 is critically involved in the shedding of FasL. In primary human T cells, FasL shedding is significantly reduced after inhibition of ADAM10. The resulting elevated FasL surface expression is associated with increased killing capacity and an increase of T cells undergoing AICD. Overall, our findings ...
In this study we examined the potential role of the FasL pathway in tumor regression by Ag-specific CD8+ CTL in vivo, which remains largely uncharacterized. One approach to understanding the mechanisms of antitumor activity is through adoptive transfer, which involves the passive administration of effector cells or established T cell lines or clones from immunized hosts to naive, tumor-bearing recipients. Furthermore, through an understanding of the nature of the antitumor immune mechanisms, adoptive transfer experiments may have implications for administration of the most appropriate functional subsets of ex vivo-expanded effector cells in anticancer therapies. Indeed, in recent clinical studies of patients with advanced metastatic melanoma, the adoptive transfer of autologous cultured tumor-infiltrating lymphocytes mediated efficient tumor regression in several patients (26), suggesting that adoptive immunotherapy is still very much a viable and relevant anticancer strategy.. Although CD8+ CTL ...
TY - JOUR. T1 - New trends in the study of podoplanin as a cell morphological regulator. AU - Sawa, Yoshihiko. N1 - Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 2010/8. Y1 - 2010/8. N2 - Podoplanin is a mucin-type glycoprotein firstly identified in podocytes, which is homologous to the type I alveolar cell specific T1α-2 antigen and to the oncofetal antigen M2A recognized by the D2-40 antibody. Podoplanin possesses a platelet aggregation-stimulating domain causes the platelet aggregation on cancer cells by the binding activity to CLEC-2. Podoplanin also contributes to the formation of membrane-actin structures. The increased podoplanin expression is found in squamous cell carcinomas at the invasive edge. It has been reported that the podoplanin induces an actin cytoskeleton rearrangement dependent on the RhoA GTPase activation to phosphorylate ezrin and facilitates an epithelial-mesenchymal transition (EMT) which induces the single cell migration of cancer cells. However, ...
Juvenile Batten disease was named after Frederick Batten, MA, MD, FRCP, when he reported on two sisters with the disease in 1903. 1, 2 As various forms of Batten were discovered, each was named according to its age of onset (Classic forms of Batten). This was very simple when there were only four known forms [infantile, late infantile, juvenile and adult]. However, several variant late forms were discovered complicating the matter. It wasnt until the first genes associated with Batten disease were discovered that researchers learned that different mistakes in the same gene can result the same disease with various ages of onset. 4, 6 In other words, mistakes in the CLN1 gene can cause disease beginning in the infantile, late infantile, juvenile or adult periods (CLN Gene Defects).. Juvenile Batten disease, the focus of Beyond Batten Disease Foundation, is also called CLN3 disease. Children with CLN3 disease almost always develop symptoms in the juvenile period.5. ...
F. Carrouel, M. J Staquet, J. F Keller, C. Baudouin, P. Msika, F. Bleicher, B. Alliot-Licht, and J. C Farges (2013) J Endod, 39(8):1008-14.. ...
Thank you for your interest in spreading the word on Circulation Research.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. ...
LBP [LPS (lipopolysaccharide)-binding protein] was discovered approximately 25 years ago. Since then, substantial progress has been made towards our understanding of its function in health and disease. Furthermore, the discovery of a large protein family sharing functional and structural attributes has helped in our knowledge. Still, key questions are unresolved, and here an overview on the old and new findings on LBP is given. LBP is an acute-phase protein of the liver, but is also synthesized in other cells of the organism. While LBP is named after the ability to bind to LPS of Gram-negative bacteria, it also can recognize other bacterial compounds, such as lipopeptides. It has been shown that LBP is needed to combat infections; however, the main mechanism of action is still not clear. New findings on natural genetic variations of LBP leading to functional consequences may help in further elucidating the mechanism of LBP and its role in innate immunity and disease. ...
Buy Anti-CD162 / P selectin glycoprotein ligand 1 / PSGL-1, clone PSGL1/1601, item number: NSJ-V3226SAF-100UG from NSJ Bioreagents at Biomol!
Human Perforin ELISA Set is intended for use in a do it yourself solid phase sandwich ELISA for the in vitro qualitative and quantitative determination of Human Perforin in supernatants, buffered solutions, serum, plasma samples.
Fas Ligand antibody [MFL4] (Fas ligand (TNF superfamily, member 6)) for FACS. Anti-Fas Ligand mAb (GTX42337) is tested in Mouse, Rat samples. 100% Ab-Assurance.
Es wird angenommen, dass an der Ätiologie der Multiplen Sklerose (MS) Umweltfaktoren beteiligt sind, die die Selbst-Toleranz gegen Auto-Antigene innerhalb des Myelins unterbrechen. Die Identität und Wirkungsweise dieser Umweltfaktoren sind aber unbekannt. Das Myelin-Oligodendrozyten-Protein (MOG) ist ein mögliches, wichtiges Auto-Antigen in der MS. Diese Arbeit zeigt, dass die Epitopspezifität von Auto-Antikörpern gegen MOG heterogen ist. Weiter wird gezeigt, dass MOG eine umfassende, immunologische Kreuzreaktivität mit dem bovinen Milch-Protein Butyrophilin (BTN) aufweist, einem ubiquitären, diätetischen Antigen. In einer Untergruppe von MS-Patienten ist diese kreuzreaktive Antikörper-Antwort signifikant erhöht und selektiv im zentralen Nervensystem (ZNS) sequestriert. Dieses Ergebnis weist darauf hin, dass diese kreuzreaktive Auto-Immunantwort eine aktive Rolle in der Pathogenese der MS spielen könnte. Möglicherweise moduliert immunologische Kreuzreaktivität zwischen MOG und BTN ...
Human Perforin ELISA Kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets
Batten disease is inherited in an autosomal recessive fashion, meaning a child needs to inherit one copy of the defective gene for the condition form each of their parents in order to develop the disease.
Batten disease is a fatal inherited disorder of the nervous system, which typically manifests between the ages of two and six. Batten disease is relatively rare, affecting 6 in every 100,000 live births in New Zealand
Currently, no effective drug is available for Batten diseases, a group of devastating disorders in children. FDA-approved lipid-lowering drugs Lopid and Tricor, in combination with vitamin A, may help patients with late infantile Batten disease.
References for Abcams Anti-Fas Ligand antibody [B-R17] (ab47174). Please let us know if you have used this product in your publication
Addy, DA and Pierce, GA and Vidovic, D and Mallick, D and Jemmis, ED and Goicoechea, JM and Aldridge, S (2010) Generation of Cationic Two-Coordinate Group-13 Ligand Systems by Spontaneous Halide Ejection: Remarkably Nucleophile-Resistant (Dimethylamino)borylene Complexes. In: Journal of the American Chemical Society, 132 (13). pp. 4586-4588. ...
Bronner F., Kleinzeller A. Current Topics in Membranes and Transport. Volume 11: Cell Surface Glycoproteins: Structure, Biosynthesis, and Biological Functions (v. 11 ...
Jadon Sancho showed his blistering pace on the counter-attack as he provided an assist to ensure Borussia Dortmund ran out winners against Wolfsburg on the second round of fixtures upon the Bundesligas return.
Gentaur molecular products has all kinds of products like :search , Alpha Dia \ Human FAS Ligand Control_blocking peptide 2 \ FASL12-P for more molecular products just contact us
A promising drug therapy that offers a ray of hope for children with Batten disease a rare neurodegenerative disease that strikes seemingly healthy kids
Learn more about Batten Disease at Grand Strand Medical Center DefinitionCausesRisk FactorsSymptomsDiagnosisTreatmentPreventionrevision ...
Plasmid pHEF-VSVG from Dr. Sergey Kasparovs lab contains the insert Vesicular Stomatitis Virus Glycoprotein and is published in Physiol Genomics. 2003 Feb 6. 12(3):221-8. This plasmid is available through Addgene.
In the laboratory, we will put a special gene into cancer cells that have been taken from the subject. This gene will make the cells produce interleukin
Ion molecule is a variety I transmembrane glycoprotein more than expressed in RB. Numerous epithelial cancers show up regulation of this protein and it has been
Buy our Recombinant Human T Plastin protein. Ab116732 is a full length protein produced in Wheat germ and has been validated in SDS-PAGE. Abcam provides free…
VCAM is a 110 kDa, cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary
The structure of membrane-bound glycoproteins is briefly discussed. Glycoproteins are associated with the cell plasma membrane. Their location on the cell surface has led to suggestions that the...
Gentaur molecular products has all kinds of products like :search , Kamiya \ Fas Ligand \ MC-111 for more molecular products just contact us