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The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
Mouse Total MMP-3 ELISA Kit,K0332183,Cytokine ELISA Kit,Mouse Total MMP-3 ELISA Kit contains all components required for the quantitative measurement of nat…
Complete information for MMP10 gene (Protein Coding), Matrix Metallopeptidase 10, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for MMP20 gene (Protein Coding), Matrix Metallopeptidase 20, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
By 240 min the transcriptome response to both protein-leucine doses implicated anti-inflammatory promyogenic biology. Reduced IL-6 expression and inhibited IL-6, TNF-α, TGF-β/SMAD, and NF-κB transcriptome networks suggest progression toward muscle regeneration (67). In murine muscle, IL-6 is an essential regulator of muscle growth involving promyogenic activation of satellite cells (61). However, no amino acids correlated with IL-6, suggesting IL-6 expression is a secondary response downstream of primary amino acid stimuli.. The ECM (i.e., endomysium) provides a structural and signaling interface between the capillary, leukocytes, and myofiber. The overrepresented ECM networks (Fig. 1, Table 1), therefore, suggest protein-leucine mediates regulation of expression of the proteinaceous ECM structural and signaling interface. Other literature evidence supports a role for postexercise protein feeding in ECM turnover, for example, expression of matrix metallopeptidase and inhibitor genes ...
MMP17 antibody [N2C2], Internal (matrix metallopeptidase 17 (membrane-inserted)) for ICC/IF, IHC-P, WB. Anti-MMP17 pAb (GTX105268) is tested in Human samples. 100% Ab-Assurance.
MMP10 antibody, N-term (matrix metallopeptidase 10) for WB. Anti-MMP10 pAb (GTX77732) is tested in Human samples. 100% Ab-Assurance.
pep chromosome:GRCm38:8:92827291:92853420:1 gene:ENSMUSG00000031740 transcript:ENSMUST00000034187 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Mmp2 description:matrix metallopeptidase 2 [Source:MGI Symbol;Acc:MGI:97009 ...
Background: Assessment of serum extracellular matrix (ECM) metalloproteinase MMP-2 and tissue inhibitor of matrix metalloproteinase TIMP-2 concentrations in non-small cell lung carcinoma (NSCLC) patients considering TNM staging. Assessment of the prognostic value of MMP-2 and TIMP-2 concentrations in NSCLC patients. Methods: The study group consisted of 81 NSCLC patients (24 females and 57 males) aged 46 to 86 years (mean age of 67±8.2 years). The control group comprised 39 randomly selected patients (20 females and 19 males) aged 27 to 73 years (mean age of 47±15 ...
BioAssay record AID 108320 submitted by ChEMBL: Inhibition of collagen IV degradation by matrix metalloprotease-9 at 10 uM (no data).
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme may degrade collagen type IV, fibronectin, fibrinogen, and beta-casein, and activate matrix metalloproteinase-9 by cleavage. The protein differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The encoded protein may promote cell invasion in multiple human cancers. [provided by RefSeq, May 2016 ...
Name of the Test: Matrix Metalloproteinase- 2 (MMP-2) Alias names: Gelatinase-A Clinical Research applications: Matrix metalloproteinases
AlphaLISA no-wash assay kit for detection and quantitation of mouse Matrix Metalloproteinase-9 (MMP9) in serum, buffered solution or cell culture medium.
BACKGROUND: 72 Kilodalton (kd) type IV collagenase is a matrix metalloproteinase that specifically cleaves type IV collagen molecules. The enzyme has been postulated to have an important role in the invasion and spread of malignant tumors. EXPERIMENTAL DESIGN: In situ hybridization was used to study the expression of the 72 kd type IV collagenase mRNA in 24 benign, 2 semimalignant, and 15 malignant ovarian tumors and in 5 metastases of ovarian serous adenocarcinomas. The results were correlated with the expression of the mRNA for the alpha 1(IV) chain of type IV collagen and with the corresponding immunohistochemical distribution of the enzyme. RESULTS: The results showed that the more malignant an ovarian tumor was, the more clearly mRNA expressions for both 72 kd type IV collagenase and the alpha 1(IV) chain could be detected in tumor cells. The expression of both types of mRNAs was localized within the cells of tumor stroma and occurred mainly in fibroblasts and vascular endothelial cells. ...
EliKine™ Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate Human MMP-9.,MMP 9; MMP-9; MMP9; Matrix Metalloproteinase 9; GELB; Type V collagenase; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase),Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Using a competitive PCR technique we have established a system for rapid and simultaneous measurement of multiple genes involved in the metabolism of the ECM within a single colonic biopsy. To gain further insight into their biological function in inflammatory bowel disease we analysed relative mRNA expression of collagen type III, matrix metalloproteinases MMP-1 and MMP-2, their respective major activators MMP-3 and MMP-14, as well as their physiological inhibitors TIMP-1 and TIMP-2 in endoscopic biopsies from inflamed and non-inflamed colon of IBD patients.. We observed that mRNA expression of all MMPs was significantly increased in inflamed compared with non-inflamed colonic mucosa of these patients. This increase was most pronounced in ulcerated colonic mucosa. By performing westerns blots, increased expression of MMP-1, MMP-2, and MMP-3 was also demonstrated at the protein level, which supports previous in vitro studies showing a close correlation between mRNA and protein expression of ...
The hydrolysis of collagen (collagenolysis) is one of the committed steps in extracellular matrix turnover. Within the matrix metalloproteinase (MMP) family distinct preferences for collagen types are seen. The substrate determinants that may guide these specificities are unknown. In this study, we have utilized 12 triple-helical substrates in combination with 10 MMPs to better define the contributions of substrate sequence and thermal stability toward triple helicase activity and collagen specificity. In general, MMP-13 was found to be distinct from MMP-8 and MT1-MMP(Delta279-523), in that enhanced substrate thermal stability has only a modest effect on activity, regardless of sequence. This result correlates to the unique collagen specificity of MMP-13 compared with MMP-8 and MT1-MMP, in that MMP-13 hydrolyzes type II collagen efficiently, whereas MMP-8 and MT1-MMP are similar in their preference for type I collagen. In turn, MMP-1 was the least efficient of the collagenolytic MMPs at processing
The hydrolysis of collagen (collagenolysis) is one of the committed steps in extracellular matrix turnover. Within the matrix metalloproteinase (MMP) family distinct preferences for collagen types are seen. The substrate determinants that may guide these specificities are unknown. In this study, we have utilized 12 triple-helical substrates in combination with 10 MMPs to better define the contributions of substrate sequence and thermal stability toward triple helicase activity and collagen specificity. In general, MMP-13 was found to be distinct from MMP-8 and MT1-MMP(Delta279-523), in that enhanced substrate thermal stability has only a modest effect on activity, regardless of sequence. This result correlates to the unique collagen specificity of MMP-13 compared with MMP-8 and MT1-MMP, in that MMP-13 hydrolyzes type II collagen efficiently, whereas MMP-8 and MT1-MMP are similar in their preference for type I collagen. In turn, MMP-1 was the least efficient of the collagenolytic MMPs at processing
matrilysin; matrin; uterine metalloendopeptidase; matrix metalloproteinase 7; putative (or punctuated) metalloproteinase-1; matrix metalloproteinase pump 1; MMP 7; PUMP-1 proteinase; PUMP; metalloproteinase pump-1; putative metalloproteinase; ...
We recently described a human cell population with progenitor-like phenotype (CD45-CD34+), resident in the white adipose tissue (WAT) and able to promote local and metastatic breast cancer (BC) progression and angiogenesis (Orecchioni et al., 2013). The molecular mechanism involved in this interaction has been so far elusive. An extensive screening of candidate molecules related to angiogenesis, inflammation, motility and invasiveness revealed that two proteins are significantly up-regulated in WAT-derived progenitors following culture with BC cells: Granulocyte-macrophage colony-stimulating factor (GM-CSF) and Matrix metallopeptidase 9 (MMP9). In vivo, both proteins were overexpressed in orthotopic models of human BC co-injected with human WAT progenitors. The inhibition of GM-CSF by a monoclonal antibody in diet-induced, obese BC mice led to a reduced intratumor vascularization and a strong impairment of WAT immunosuppressive microenvironment, targeting mainly myeloid cells such as macrophages ...
72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene. The MMP2 gene is located on chromosome 16 at position 12.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from ...
Results By 1 year 103 patients had died and 173 patients had died or been readmitted with HF. MMP-3 was significantly greater in males (p , 0.001), those with hypertension (p , 0.01) and with a past history of chronic HF (p = 0.01) and chronic kidney disease (CKD) (p , 0.001). MMP-3 was significantly correlated with age (p , 0.001) while being inversely correlated with eGFR (p , 0.001) and heart rate (p , 0.008). MMP-3 was significantly greater in patients with a LVEF , 40% compared with those with EF , 40% (p = 0.017). MMP-3 levels were significantly greater in the 108 patients who died compared to those who did not [median = 29.7 [19. to 41.1] vs. 18.9 [11.8 to 30.7] pg/ml, p , 0.001) as well as in those with the combined endpoint compared to those without (26.1 [16.7 to 36.4] vs. 17.9 [11.4 to 31.3] pg/ml, p , 0.001). In Kaplan-Meier analysis, patients with above median MMP-3 levels were significantly more likely to experience the endpoint (p , 0.001) compared to those who did not.. Discharge ...
Expression of matrix metalloproteinase 13 (MMP-13) in human rheumatoid arthritis (RA) (A) synovial tissue (magnification ×200) and (B) the isotype control (mag
Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
This Anti-MMP-9 Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of MMP-9. - Find MSDS or SDS, a COA, data sheets and more information.
In our previous study, we have reported that brain AVM samples had higher levels of MMP-9,TIMP-1, and TIMP-3. MMP-2 and TIMP-2 levels were below detection in brain AVMs.(22)MMP-9 has been implicated to play a critical role in vascular remodeling and rupture, thus wefocused on MMP-9 in this study. Our data indicate that MMP-9 derived from the blood poolcontributes only to a small degree to the otherwise large amount of total MMP-9 expressed inbrain AVM tissue amount, as determined by the ELISA method. This notion is supported byour animal study which showed that increasing dosage of blood only partially elevated MMP-9level. MMP-9 was determined at 3 hr after blood infusion. We chose this time point to mimicthe period for AVM tissue harvest at clinical settings. This is also the time period for neutrophil in the blood to infiltrate and transmigrate to vascular wall (31). Infusion of whole blood intothe mouse brain increased MMP-9 level to 2.4 ng/mg compared to1.6 ng/mg with normal salineinjection. ...
Experimental evidence suggests that matrix metalloproteinase-13 (MMP-13) protein may promote breast tumor progression. Bin Zhang, Xuchen Cao, Yanxue Liu, W
PubMed journal article Overexpression of matrix metalloproteinase-9 (MMP-9) rescues insulin-mediated impairment in the 5XFAD model of Alzheimers diseas were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
This is the first study to describe the role of MMP-2 and MMP-9 in Hodgkins disease. Strong MMP-2 expression correlated with a favorable prognosis, while MMP-9 expression showed a tendency toward an adverse outcome. MMP-9 expression correlated with B symptoms and decreased new vessel formation. MMP-2 expression was associated with the nodular sclerosis subtype, and its expression was most pronounced in the vicinity of sclerosis. Neither of the gelatinases nor the extent of neovascularization correlated with tumor stage, the occurence of bulky disease, or extranodal infiltrates. Together, these findings imply that the adverse role of MMP-9 may be associated with the controlling of immunological processes but not the invasion probabilities or neovascularization of the tumor. The favorable prognostic value of MMP-2 is surprising in view of the role of MMPs in solid tumors. This, however, may be linked to the basic biological differences of hematological malignancies vs. other tumors ...
Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form. Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collag
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Sigma-Aldrich offers abstracts and full-text articles by [Taís M Campos, Sara T Passos, Fernanda O Novais, Daniel P Beiting, Rúbia S Costa, Adriano Queiroz, David Mosser, Phillip Scott, Edgar M Carvalho, Lucas P Carvalho].
Effect of interleukin (IL)-17A and IL-17E on the production of matrix metalloproteinase (MMP)-3, MMP-12, and tissue inhibitor of metalloproteinase (TIMP)-1 by i
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EK2821小鼠基质金属蛋白酶1(MMP-1)ELISA试剂盒Mousematrixmetalloproteinase1,MMP-1ELISAkitEK2822小鼠基质金属蛋白酶10(MMP-10)ELISA试剂盒Mousematrixmetalloproteinase10,MMP-10ELISAkitEK2823小鼠基质金属蛋白酶13(MMP-13)ELISA试剂盒Mousematrixmeta
Some of the MMPs seem to be expressed in all or almost all traumatic synovial membrane samples. Traumatic synovial membrane is naturally not normal synovial membrane. However, this may imply a constitutive expression pattern and a role in normal tissue remodelling processes. These MMPs were MMP-2 (gelatinase A or 72 kDa type IV collagenase), MMP-3 (stromelysin-1), MMP-11 (stromelysin-3) and MMP-19. Their constitutive expression does not mean that they could not play a part in pathological tissue destruction, for example, because of their increased synthesis in diseases and/or insufficient regulation of their degradative potential. For example, MMP-2 has recently been found to be part of a cell membrane associated ternary MT1-MMP/TIMP-2/MMP-2 complex, which may contribute to proMMP-2 activation and focused, pericellular targeting of its action.20 In addition, MMP-2 is, not only gelatinase, but also a collagenase active against interstitial type I and II collagens.21 22 Some MMP mRNAs were found ...
TY - JOUR. T1 - The effects of omega-3 fatty acids on matrix metalloproteinase-9 production and cell migration in human immune cells. T2 - Implications for multiple sclerosis. AU - Shinto, Lynne. AU - Marracci, Gail. AU - Bumgarner, Lauren. AU - Yadav, Vijayshree. PY - 2011. Y1 - 2011. N2 - In multiple sclerosis (MS), compromised blood-brain barrier (BBB) integrity contributes to inflammatory T cell migration into the central nervous system. Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS. The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration. Peripheral blood mononuclear cells (PBMC) from healthy controls were pretreated with two types of omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Cell supernatants were used to determine MMP-9 protein and activity levels. Jurkat cells were pretreated with EPA and DHA and were added to fibronectin-coated ...
Prediction on the Inhibition Ratio of Pyrrolidine Derivatives on Matrix Metalloproteinase Based on Gene Expression Programming. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Paper I: Furenes EB, Seljeflot I, Solheim S, Hjerkinn EM, Arnesen H. Long-term influence of diet and/or omega-3 fatty acids on matrix metalloproteinase-9 and pregnancy-associated plasma protein-A in men at high risk of coronary heart disease. Scand J Clin Lab Invest 2008; 68: 177-184. The paper is removed from the thesis in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1080/00365510701663350 ...
Increased expression of tissue factor (TF) is associated with tumor invasion and metastasis in human colorectal cancer. We have previously observed that TF/FVIIa upregulates matrix metalloproteinase-7 (MMP-7) expression at the transcriptional level in colon cancer cells. MMP-7 overexpression is believed to play an important role in tumor invasion and metastasis. The aim of this study is to elucidate the molecular mechanisms by which TF/FVIIa induced MMP-7 expression and cell invasion in vitro.. Reverse transcription polymerase chain reaction, Western blot, luciferase assay, and chromatin immunoprecipitation (ChIP) were used to determine the potential mechanism and signaling pathways by which TF/FVIIa induced MMP-7 expression and cell invasion in LoVo cells. Small interfering RNA (siRNA) and cell invasion assay was used to examine whether blocking c-Fos expression could abolish FVIIa-mediated upregulation of MMP-7 and cell invasion in vitro.. The results showed that FVIIa induced the upregulation ...
Title: Matrix Metalloproteinase-9 and Airway Remodeling in Asthma. VOLUME: 4 ISSUE: 2. Author(s):Hiroyuki Ohbayashi and Kaoru Shimokata. Affiliation:Department of Internal Medicine, Tohno-Kousei Hospital, 76-1, Toki-cho, Mizunami City, Gifu Pref. 509-6101, Japan.. Keywords:matrix metalloproteinase, asthma, the extracellular matrix, tissue inhibitor of metalloproteinase. Abstract: Airway remodeling is a major change responsible for irreversible asthmatic airflow restriction. The Th-2 cytokines-dominant eosinophilic inflammatory mechanism cannot fully explain the progressive subepithelial fibrosis and structural changes in the extracellular matrix (ECM). Matrix metalloproteinases (MMPs) are the key enzymes responsible for ECM degradation. MMPs are normally produced and secreted under the tight regulation of, at least, 3 different levels: the gene transcriptional level, the activation of the latent form of enzyme, and the inactivation by specific endogenous inhibitors. In asthmatic condition, as ...
Background: Mental stress-induced myocardial ischemia (MSIMI) is associated with increased risk of adverse cardiovascular outcomes, yet the underlying mechanisms are not well understood. We measured the inflammatory response to acute laboratory mental stress in patients with coronary artery disease (CAD) and its association with MSIMI. We hypothesized that patients with MSIMI would have a higher inflammatory response to mental stress in comparison to those without ischemia. Methods: Patients with stable CAD underwent 99mTc sestamibi myocardial perfusion imaging during mental stress testing using a public speaking stressor. MSIMI was determined as impaired myocardial perfusion using a 17-segment model. Inflammatory markers including interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), matrix metallopeptidase 9 (MMP-9) and high-sensitivity C reactive protein (hsCRP) were measured at rest and 90 min after mental stress. Results were validated in an independent sample of 228 ...
TY - JOUR. T1 - Adhesion induces matrix metalloproteinase-9 gene expression in ovarian cancer cells. AU - Yan, Chunhong. AU - Tian, Fang. AU - Xiao, Fengjun. AU - Li, Keqin. AU - Li, Chunhai. PY - 2002/1/1. Y1 - 2002/1/1. N2 - OBJECTIVE: This work was conducted to investigate the expression of matrix metalloproteinase 9 (MMP 9) gene in cancer cells by fibronectin adhesion and the underlying mechanism of cell invasion. METHODS: Following adhesion of ovarian cancer cells A2780 to fibronectin, mRNA expression of MMP cells were assayed by reverse transcription-polymerase chain reaction (RT-PCR). MMP9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells. RESULTS: Adhesion induced the increase of cellular MMP9 mRNA content in A2780 cells, not affecting the expression of MMP2 or TIMP-1 gene. The stimulation was enhanced with the increase adhesion time. When the transfected cells were allowed ...
A total of 73 CRC patients who underwent MICR met the inclusion criteria (34 males /39 female, mean ages 66.3± 12.8 years; 28% rectal and 62% colon lesions). The mean incision length was 7.8 ± 3.6cm and mean length of stay was 6.8± 4.4 days. The final cancer staging breakdown was; Stage I, 27%, Stage II, 33%, stage III, 36% and stage IV, 4%. The mean PreOp MMP3 level was 14.9±7.8 ng/ml (n=73). Significantly elevated mean plasma levels were noted on POD1 (21.4±14.7ng/ml, n= 73, p=, 0.0001), POD3 (37.9±21.5 ng/ml, n= 72, p=, 0.0001), POD7-13 (22.0±13.0 ng/ml, n=56, p=, 0.0001), POD14-20 (21.9±10.3 ng/ml, n=20, p=0.003) and on POD 21-27 (21.9±11.43 ng/ml, n= 20, p=0.002) when compared to PreOp levels. Plasma levels returned to the PreOp baseline at the POD 28-41 time point (n=16, p=0.07).. Conclusion ...
In this study, Compritol ATO-based Resveratrol colloidal carriers (CCCs) were prepared. In most formulae, the use of a binary-mixture of surfactants improved the physicochemical properties. CCC6 (containing P407/P188 as bi-surfactants) attained the highest drug loading, release efficiency during 24 hr and occlusive effect for 48 hr; in addition to, a uniform particle size distribution within the desired range. In-vivo studies were done based on the analysis of anti-oxidant markers [catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD)], anti-inflammatory markers [interleukin 6 (IL-6), interleukin 8 (IL-8) and rat Nuclear factor-kappa B (NF-κB)] and anti-wrinkling markers [matrix metalloproteinase (MMP-1) and Granulocyte-macrophage colony-stimulating factor (GM-CSF)], after UVB-irradiation ...
History The upregulation of matrix metalloproteinase-1 (MMP-1) continues to be proven correlated with lymph node metastasis of nasopharyngeal carcinoma (NPC) as the activation of protease-activated receptor-1 (PAR-1) mediates proliferation and invasion of NPC cells. in 190 (71.43%) and 182 (68.42%) from the 266 NPC sufferers. Furthermore the mixed MMP-1 and PAR-1 appearance was significantly connected with advanced T-stage (= 0.01) advanced clinical stage (= 0.002) positive recurrence (= 0.01) and metastatic position (= 0.01) of NPC. Furthermore the overall success in NPC sufferers MAP3K8 with MMP-1 and PAR-1 dual overexpression was considerably shorter than in people that have dual low appearance (< 0.001). Furthermore the multivariate analyses indicated the fact that mixed MMP-1 and PAR-1 overexpression was an unbiased prognostic aspect for overall success (= 0.001) in NPC sufferers however the upregulation of MMP-1 and PAR-1 alone is at each case no independent prognostic aspect because of ...