1FLS: High-resolution solution structure of the catalytic fragment of human collagenase-3 (MMP-13) complexed with a hydroxamic acid inhibitor.
1FM1: High-resolution solution structure of the catalytic fragment of human collagenase-3 (MMP-13) complexed with a hydroxamic acid inhibitor.
Local resource for knee injury specialists in Baldwinsville. Includes detailed information on local clinics that provide access to orthopedic doctors, as well as advice and content on knee injury, sports medicine, and knee surgery.
Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Interleukin-8 (IL-8), a chemokine with a defining CXC amino acid motif, is known to possess tumorigenic and proangiogenic properties. Over-expression of IL-8 has been detected in many human tumors. However, the effects of IL-8 in migration and integrin expression in chondrosarcoma cells are largely unknown. In this study, we found that IL-8 increased the migration and the expression of αvβ3 integrin in human chondrosarcoma cells. Activations of phosphatidylinositol 3-kinase (PI3K), Akt, and AP-1 pathways after IL-8 treatment were demonstrated, and IL-8-induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and AP-1 cascades. Taken together, our results indicated that IL-8 enhances the migration of chondrosarcoma cells by increasing αvβ3 integrin ...
Osteoarthritis (OA) is a debilitating disease of the joints characterized by cartilage degradation but to date there is no available pharmacological treatment to inhibit disease progression neither is there any available biomarker to predict its development. In the present study, we examined the expression level and possible involvement of novel cell-ECM adhesion-related molecules such as Iintegrin Linked Kinase (ILK), PINCH, parvin, Mig-2 and Migfilin in OA pathogenesis using primary human articular chondrocytes from healthy individuals and OA patients. Our findings show that only ILK and Migfilin were upregulated in OA compared to the normal chondrocytes. Interestingly, Migfilin silencing in OA chondrocytes rather exacerbated than ameliorated the osteoarthritic phenotype, as it resulted in even higher levels of catabolic and hypertrophic markers while at the same time induced reduction in ECM molecules such as aggrecan. Furthermore, we also provide a link between Migfilin and beta-catenin ...
Invitrogen™ eBioscience™ Human MMP-9 Platinum ELISA Kit 96 tests Invitrogen™ eBioscience™ Human MMP-9 Platinum ELISA Kit L-M ELISA Kits
Assay Kits , MMP Assay Kits , Human MMP-7, Recombinant; Matrix metalloproteinases (MMP s) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. This recombinant human MMP-7, expressed as the pro-enzyme, becomes highly active when incubated with APMA. It can be used in FRET-based enzyme activity assay or served as positive control for Western blot, IP, and ELISA.
A great deal of effort has been focused on exploring the underlying molecular mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of this investigation is to identify molecular features and the stage of OA based on the spectral signatures corresponding to bio-molecular changes at the cellular level in chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage and acquired Raman spectra from single cells. Major spectral differences between the cells obtained from different International Cartilage Repair Society (ICRS) grades of osteoarthritic cartilage were identified. During progression of OA, a decrease in protein content and an increase in cell death were observed from the vibrational spectra. Principal component analysis and subsequent cross-validation was able to associate osteoarthritic chondrocytes to ICRS Grade I, II and III with ...
TY - JOUR. T1 - Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes. AU - Su, S. L.. AU - Tsai, C. D.. AU - Lee, C. H.. AU - Salter, D. M.. AU - Lee, Herng Sheng. PY - 2005/10. Y1 - 2005/10. N2 - Objective: The objective of this study was to examine expression and regulation of Toll-like receptor 2 (TLR2) in human articular chondrocytes. Methods: Human articular chondrocytes were enzymatically isolated from normal and osteoarthritic knee cartilage. Immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess the expression of toll-like receptors. Following stimulation of chondrocytes in vitro by IL-1β and fibronectin proteolytic fragments, the relative levels of mRNA for TLR2 were determined by quantitative real-time PCR. MyD88 activation and nuclear factor-κB (NF-κB) translocation were evaluated by immunoprecipitation and electrophoretic mobility shift assay, ...
Background: Recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. Integrin signaling may be regulated by a number of mechanisms including accessory proteins such as CD98 (4F2 antigen). Objectives: To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechanotransduction. Methods: CD98 expression was assessed by immunostaining of cryostat sections of snap frozen articular cartilage and in cultured cells by western blotting. Chondrocytes enzymatically isolated from macroscopically normal and osteoarthritic (OA) articular cartilage were grown in short term, primary monolayer culture and used in a resting state or following mechanical stimulation at 0.33Hz. Results: Human articular chondrocytes express CD98 and immunoreactivity revealed a similar heterogeneous pattern of CD98 in both normal and osteoarthritic (OA) human articular cartilage. No role of CD98 was detected
Cyclooxygenase (COX)-2, the inducible isoform of prostaglandin (PG) synthase, has been implicated in tumor metastasis. Interaction of COX-2 with its specific EP receptors on the surface of cancer cells has been reported to induce cancer invasion. However, the effects of COX-2 on migration activity in human chondrosarcoma cells are mostly unknown. In this study, we examined whether COX-2 and EP interaction are involved in metastasis of human chondrosarcoma. We found that over-expression of COX-2 or exogenous PGE2 increased the migration of human chondrosarcoma cells. We also found that human chondrosarcoma tissues and chondrosarcoma cell lines had significant expression of the COX-2 which was higher than that in normal cartilage. By using pharmacological inhibitors or activators or genetic inhibition by the EP receptors, we discovered that the EP1 receptor but not other PGE receptors is involved in PGE2-mediated cell migration and α2β1 integrin expression. Furthermore, we found that human
BioAssay record AID 108320 submitted by ChEMBL: Inhibition of collagen IV degradation by matrix metalloprotease-9 at 10 uM (no data).
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TY - JOUR. T1 - Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes. T2 - An in vitro study using baicalein. AU - Wu, Chang Chin. AU - Chen, Yi Ru. AU - Lu, Dai Hua. AU - Hsu, Li Ho. AU - Yang, Kai Chiang. AU - Sumi, Shoichiro. PY - 2020/6. Y1 - 2020/6. N2 - Introduction: Targeting inflammatory cascades is considered a promising way to prevent knee osteoarthritis (OA) progression. In terms of down-regulating the expression of inducible nitric oxide synthase (iNOS), interleukin (IL)-6, and matrix metalloproteinases (MMPs), pre-treatment with the flavonoid baicalein reportedly protects articular chondrocytes against the cytotoxicity of IL-1β. However, the benefits of post-treatment baicalein on osteoarthritic chondrocytes are not fully elucidated. Methods: In this study, primary human chondrocytes were stimulated with IL-1β prior to baicalein application to evaluate the therapeutic effect of post-treatment. Results: Post-treatment baicalein alleviated cell ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
Synergistic induction of matrix metalloproteinase 1 by interleukin-1 alpha and oncostatin M in human chondrocytes involves signal transducer and activator of transcription and activator protein 1 transcription factors via a novel ...
Mouse anti Human MMP-9 Activated antibody, clone 4A3 recognizes the active form of human matrix metalloproteinase 9 (MMP-9). The MMPs are
Curcumin exerts anti-apoptotic and anti-catabolic effects on IL-1beta-stimulated articular chondrocytes, indicating is may have therapeutic value in the treatment of osteoarthritis and related osteoarticular disorders.
Recent in vitro studies showed a positive direct effect of celecoxib, one of the selective COX-2 inhibitors, on human OA cartilage. Such effects are difficult to verify in a clinical trial because changes in OA cartilage, degenerative and reparative, are slow and evaluation of articular cartilage by imaging techniques is still hampered by their limited sensitivity ...
Loss of articular cartilage from ageing, injury or degenerative disease is commonly associated with inflammation, causing pain and accelerating degradation of the cartilage matrix. Sulphated glycosaminoglycans (GAGs) are ...
Increased inflammation delays wound healing in mice deficient in collagenase-2 (MMP-8).: Matrix metalloproteinases (MMPs) have been implicated in numerous tissu
Methods. OA chondrocytes were stimulated with AGE-modified BSA (AGE-BSA). Gene expression of IL-6 and IL-8 was quantified by TaqMan assays and the production ...
In order to be able to use second-generation ACT techniques for the repair of cartilage defects in patients with OA, it is highly important to investigate whether OA chondrocytes have an irreversibly altered phenotype or if these cells can differentiate towards a hyaline cartilage phenotype after in vitro expansion. Today, there are conflicting data whether OA chondrocytes fulfill the prerequisites for ACT treatment or not [12, 13, 15, 21]. This encouraged us to investigate more thoroughly the chondrogenic differentiation potential of human OA chondrocytes using microarray technology in order to determine whether OA chondrocytes might possibly be used in second-generation ACT.. Microarray analysis of human OA and ND chondrocytes cultured in ML indicated that the OA chondrocytes were in a less differentiated state compared with the ND chondrocytes. This is thus in accordance with the differences detected in vivo between OA and ND cartilage [10, 22]. Re-differentiation in scaffold cultures ...
In recent years it has become increasingly clear that articular cartilage harbours a viable pool of progenitor cells and interest has focussed on their role during development and disease. Analysis of progenitor numbers using fluorescence-activated sorting techniques has resulted in wide-ranging estimates, which may be the result of context-dependent expression of cell surface markers. We have used a colony-forming assay to reliably determine chondroprogenitor numbers in normal and osteoarthritic cartilage where we observed a 2-fold increase in diseased tissue (P , 0.0001). Intriguingly, cell kinetic analysis of clonal isolates derived from single and multiple donors of osteoarthritic cartilage revealed the presence of a divergent progenitor subpopulation characterised by an early senescent phenotype. Divergent sub-populations displayed increased senescenceassociated β-galactosidase activity, lower average telomere lengths but retained the capacity to undergo multi-lineage differentiation. ...
In vitro and animal model of osteoarthritis (OA) studies suggest that TGF-β signalling is involved in OA, but human data is limited. We undertook this study to elucidate the role of TGF-β signalling pathway in OA by comparing the expression levels of TGFB1 and BMP2 as ligands, SMAD3 as an intracellular mediator, and MMP13 as a targeted gene between human osteoarthritic and healthy cartilage. Human cartilage samples were collected from patients undergoing total hip/knee joint replacement surgery due to primary OA or hip fractures as controls. RNA was extracted from the cartilage tissues. Real-time quantitative PCR was performed to measure gene expression. Mann-Whitney test was utilized to compare the expression levels of TGFB1, BMP2, SMAD3 and MMP13 in human cartilage between OA cases and controls. Spearmans rank correlation coefficient (rho) was calculated to examine the correlation between the expression levels of the four genes studied and non-parametric regression was used to adjust for covariates
Human chondrosarcomas do not respond to current chemotherapies or radiation therapy, and their size and histological appearance do not reliably predict the risk of local recurrence and metastases, making selection of surgical treatment difficult. Identifying mechanisms responsible for the proliferation and invasive behavior of these tumors would be of immense clinical value. We hypothesized that telomerase expression is one of these mechanisms. We detected telomerase expression in 7 of 16 chondrosarcomas, but cells cultured from telomerase-negative chondrosarcomas acquired strong telomerase activity and lost tumor suppressor activity after their establishment in culture. These changes were associated with accelerated indefinite cell proliferation, morphological transition, and increased invasive activity, indicating that telomerase activation and loss of cell cycle control leads to the emergence of aggressive cells from chondrosarcoma cell populations. These observations may lead to better ...
Platelet-rich plasma releasate counteracts effects of an inflammatory environment on genes regulating matrix degradation and formation in human chondrocytes. Platelet-rich plasma releasate decreases NFκB activation, a major pathway involved in the pathogenesis of OA. These results encourage further …
BioAssay record AID 712141 submitted by ChEMBL: Prodrug conversion assessed as recombinant human MMP-12 mediated compound conversion to(S)-5-((S)-1-amino-1-oxopropan-2-ylamino)-4-((S)-2-((S)-2-(2-(biphenyl-4-ylsulfonamido)acetamido)-4-methylpentanamido)-4-carboxybutanamido)-5-oxopentanoic acid by HPLC analysis.
What is Keratoacanthomas -symptoms, pictures, causes, treatment. It normally affects the skin of middle-aged and older individuals which has been chronically
Recent studies of OA cartilage have identified both messenger RNA (mRNA) and the protein for specific MMPs as well as a collagenase mediated type II collagen degradation product, suggesting that MMPs contribute to the intrinsic chondrocyte mediated degenerative changes of the cartilage matrix in OA.8,12,13 As yet the factors responsible for their expression remain uncertain, although the proinflammatory cytokines interleukin 1 (IL1) and tumour necrosis factor α (TNFα) have been implicated.1,8 The increased levels of histamine found in OA synovial fluids3 have suggested a role for this mediator in the pathophysiology of this disease. Evidence presented here shows that histamine up regulates both MMP-13 and MMP-3 production by chondrocytes. Both these MMPs are important in the degradation of articular cartilage; MMP-13 can degrade collagen type II, and MMP-3 can degrade proteoglycan and collagen types IX and XI, and activate procollagenase-1.14 Earlier studies have shown that chondrocytes ...
The matrix metalloproteinases (MMPs) are family of enzymes with a common domain structure that degrade extracellular matrix proteins; these enzymes are overexpressed in numerous diseases including cancer and arthritis. Collagenase-1, 2, 3 (MMP-1, 8, 13) and MT1-MMP are the only known enzymes capable of cleaving triple helical collagens Type-I, II, and III: thus, their presence is the rate limiting step for proteolytic cleavage of collagens. The collagens are the most abundant proteins in the body, providing structural and tensile strength to tissues. Proteolytic degradation of collagens in cartilage, tendon, and bone cause irreversible joint destruction in arthritis. In Osteoarthritis, slow cartilage degradation results from chondrocyte proteinase secretion and cleavage of type-II collagen. Collagenase-3 (MMP-13) can digest a wide range of matrix components, including type-II collagen in cartilage and its expression co-localizes with active osteoarthritic lesions, The expression of MMP-13 in ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
We initially identified BATF as an upregulated transcription factor in chondrocytes stimulated with IL-1β, IL-6 and TNF-α, the major proinflammatory cytokines in OA pathogenesis. IL-1β is associated with cartilage destruction and TNF-α with driving the inflammatory cascade.4 IL-6 has been shown to induce MMP3 and MMP13 protein expression, resulting in OA cartilage destruction in mice.15 In view of the finding that these cytokines enhance BATF expression in chondrocytes, we investigated the specific functions of BATF in OA pathogenesis. Our gain-of-function (IA injection of Ad-Batf and Batf TG mice) and loss-of-function (Batf KO mice and IA injection of AP-1 inhibitor) studies clearly indicate that BATF is a catabolic regulator of OA pathogenesis. We employed conventional Batf−/− rather than cartilage-specific KO mice for the experiments since OA is considered a whole-joint disease involving multiple pathological changes in different cells of joint tissue.1 Therefore, deletion of Batf in ...
We previously advanced the hypothesis that a highly regulated balance of synthesis and degradation determines collagen content in the fibrous cap of atherosclerotic plaques.1,2 In turn, collagen levels critically influence the integrity of the plaques cap, a structure whose biomechanical failure may cause most myocardial infarctions. Earlier indirect evidence suggested that collagenases of the MMP family can regulate collagen content in the plaque.5-12 We initially demonstrated overexpression of the prototypical interstitial collagenase MMP-1 in human atheromata5 and later showed colocalization of MMP-1/collagenase-1 and MMP-13/collagenase-3 with degraded collagen in these lesions as detected by an antibody specific for the collagenase cleavage site of collagen.9 Recently, our group showed that human atheromata contain a third interstitial collagenase, MMP-8/collagenase-2,11 also present in mouse atheromata, as shown here (Figure 2C). Shah et al32 reported that conditioned media of cultured ...
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes
if you cant have surgery, or if you have multiple keratoacanthomas, you can try other treatments, such as: * the chemotherapy drug 5-fluorouracil. you can get it as a cream to rub on your skin or as
Angele, P; Faltermeier, H; Kujat, R; Maghsudi, M; Möller, H D; Nerlich, M; (1998) [Improvement of the amplification rate of human chondrocytes with IGF-I and RGD]. Langenbecks Archiv für Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft für Chirurgie. Kongress, 115 (Suppl). pp. 205-8. ISSN 0942-2854 https://researchonline.lshtm.ac.uk/id/eprint/19907 Full text not available from this repository ...
OBJECTIVE: To determine whether, in human fibroblasts and chondrosarcoma cells, the regulation of interleukins (IL)-6, 8, and 11 and matrix metalloproteinases (MMP)-1, 3, and 13, and their tissue inhibitor TIMP-1, depends on the transcription factor nuclear factor-kappaB (NF-kappaB). METHODS: Fibroblasts and chondrosarcoma cells were effectively infected with an adenovirus encoding human IkappaBalpha, and inhibition of NF-kappaB function was observed. The induction of MMP and IL-6, 8, and 11 by various stimuli was assessed by ELISA. RESULTS: The induction of IL-6 and IL-8 clearly depended on NF-kappaB in both fibroblasts and chondrosarcoma cells, irrespective of stimulus, but IkappaBalpha overexpression had little effect on IL-11. MMP-1, -3, and -13 were also inhibited, but TIMP-1 was unaffected. CONCLUSION: NF-kappaB appears to play an important and selective role in MMP induction in human fibroblasts and chondrosarcoma cells. This suggests there are NF-kappaB dependent mechanisms of cartilage ...
In this study, we investigated the anticancer effects of a new benzimidazole derivative, 1-benzyl-2-phenyl -benzimidazole (BPB), in human chondrosarcoma cells. BPB-mediated apoptosis was assessed by the MTT assay and flow cytometry analysis. The in vivo efficacy was examined in a JJ012 xenograft model. Here we found that BPB induced apoptosis in human chondrosarcoma cell lines (JJ012 and SW1353) but not in primary chondrocytes. BPB induced upregulation of Bax, Bad and Bak, downregulation of Bcl-2, Bid and Bcl-XL and dysfunction of mitochondria in chondrosarcoma. In addition, BPB also promoted cytosolic releases AIF and Endo G. Furthermore, it triggered extrinsic death receptor-dependent pathway, which was characterized by activating Fas, FADD and caspase-8. Most importantly, animal studies revealed a dramatic 40% reduction in tumor volume after 21 days of treatment. Thus, BPB may be a novel anticancer agent for the treatment of chondrosarcoma.
Define keratoacanthomas. keratoacanthomas synonyms, keratoacanthomas pronunciation, keratoacanthomas translation, English dictionary definition of keratoacanthomas. Noun 1. keratoacanthoma - skin tumor that grows rapidly and resembles a carcinoma but does not spread; it usually disappears spontaneously, often leaving a...
matrilysin; matrin; uterine metalloendopeptidase; matrix metalloproteinase 7; putative (or punctuated) metalloproteinase-1; matrix metalloproteinase pump 1; MMP 7; PUMP-1 proteinase; PUMP; metalloproteinase pump-1; putative metalloproteinase; ...
In recent years, intraarticular inflammation has been recognized to contribute to the symptoms and progression of osteoarthritis (OA). Inflammation in OA is associated with increased levels of catabolic enzymes and inflammatory mediators such as nitric oxide (NO), interleukin-1ß (IL-1ß) and tumor necrosis factor-α (TNF-α). Chondrocytes and infiltrating inflammatory cells, neurtrophils and macrophages, participate in the production of these catabolic mediators [1]. Extract from the heartwood of Caesalpinia sappan and its active components were reported to have antioxidative, antibacterial and anti-inflammatory effects [2]. The present study was designed to investigate whether ethanolic extract of C. sappan possesses anti-inflammatory activities in an in vitro model of joint inflammation comprising primary human osteoarthritic chondrocytes and differentiated THP-1 macrophage cells.
Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
The AlphaLISA® immunoassay kit for human matrix metalloproteinase 23 (MMP-23) enables the quantitative determination of human MMP-23 in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps ...
Zamani, S. and Dehghani, Leila. and Drummen, G. and esfandiari, Ebrahim. and Abutorabi, Roshanak. and Rabbani, Hossein. and Tahani, Soheil. and Hashemi beni, Batool. (2016) Comparison of cartilage specific markers in articular and differentiated chondrocytes in pellet system. Biointerface Research in Applied Chemistry, 6 (6). Zamani, Saeed. and Hashemibeni, Batool. and esfandiari, Ebrahim. and Kabiri, Azadeh. and Rabbani, Hossein. and Abutorabi, Roshanak. (2014) Assessment of TGF-β3 on production of aggrecan by human articular chondrocytes in pellet culture system. Advanced Biomedical Research. ...
EK2821小鼠基质金属蛋白酶1(MMP-1)ELISA试剂盒Mousematrixmetalloproteinase1,MMP-1ELISAkitEK2822小鼠基质金属蛋白酶10(MMP-10)ELISA试剂盒Mousematrixmetalloproteinase10,MMP-10ELISAkitEK2823小鼠基质金属蛋白酶13(MMP-13)ELISA试剂盒Mousematrixmeta