Marinobacter aquaeolei ATCC ® 700491D-5™ Designation: Genomic DNA from Marinobacter aquaeolei strain VT8 TypeStrain=True Application:

On the basis of phenotypical characteristics and analysis of 16S rRNA sequence, a new species belonging to a new genus is described, and the name Marinobacter hydrocarbonoclasticus is proposed. This organism, isolated from Mediterranean seawater near a petroleum refinery, is a gram-negative, aerobic, rod-shaped bacterium. It grows at NaCl concentrations of 0.08 to 3.5 M and uses various hydrocarbons as the sole source of carbon and energy. Its DNA has a guanine-plus-cytosine content of 52.7 mol%. The 16S rRNA analysis shows a clear affiliation between M. hydrocarbonoclasticus and the gamma group of the phylum Proteobacteria. A close phylogenetic relationship appears among the species Marinomonas vaga, Oceanospirillum linum, Halomonas elongata, and Pseudomonas aeruginosa. Because of the impossibility of finding a single most closely related species, we suggest that this bacterium be assigned to a new genus, at least temporarily. The possibility of a revision of this status when new data appear is,

General Information: Marinobacter aquaeolei VT8, also known as Marinobacter hydrocarbonoclasticus VT8, is a moderately halophilic, hydrocarbon-degrading bacterium isolated from an oil well off the coast of Vietnam. Hydrocarbon-degrading marine bacterium. This organism is a moderately halophilic, hydrocarbon-degrading bacterium which has been isolated from a number of hydrocarbon polluted marine environments. Marinobacter hydrocarbonoclasticus is able to produce biofilms as well as survive in open seawater. ...

A Gram-negative marine bacterium, designated strain En6T, was isolated from seawater of the East Sea of Korea. The organism grew in 1-25 % (w/v) NaCl and at 10-37 °C and pH 5.3-9.3, with optimal growth occurring in 4-5 % NaCl and at 25-30 °C and pH 7.5. Phylogenetic analysis of the 16S rRNA gene sequence of strain En6T placed this bacterium in the clade Marinobacter within the class Gammaproteobacteria. The 16S rRNA gene sequence similarity between strain En6T and Marinobacter lipolyticus SM19T, the most closely related species, was 98.4 %, and the level of DNA-DNA relatedness between the two strains was 22 %. On the basis of the phylogenetic analysis and phenotypic and chemotaxonomic data, strain En6T is considered to represent a novel species of the genus Marinobacter. The name Marinobacter goseongensis sp. nov. is proposed, with strain En6T (=KCTC 12515T=DSM 19471T) as the type strain.

As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Microorganisms are capable of producing advanced biofuels that can be used as drop-in alternatives to conventional liquid fuels. However, vital physiological processes and membrane properties are often disrupted by the presence of biofuel and limit the production yields. In order to make microbial biofuels a competitive fuel source, finding mechanisms for improving resistance to the toxic effects of biofuel production is vital. This investigation aims to identify resistance mechanisms from microorganisms that have evolved to withstand hydrocarbon-rich environments, such as those that thrive near natural oil seeps and in oil-polluted waters. First, using genomic DNA from Marinobacter aquaeolei, we constructed a transgenic library that we expressed in Escherichia coli. We exposed cells to inhibitory levels of pinene, a monoterpene that can serve as a jet fuel precursor with chemical properties similar to existing tactical fuels. Using a sequential strategy of a fosmid library followed by a plasmid

SWISS-MODEL Repository entry for A1TYK3 (RS3_MARHV), 30S ribosomal protein S3. Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

SWISS-MODEL Repository entry for A1U3F6 (DDL_MARHV), D-alanine--D-alanine ligase. Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

Pelagianism is the belief that original sin did not taint human nature and that mortal will is still capable of choosing good or evil without special divine aid. This theological theory is named after the British monk Pelagius (354-420 or 440), although he denied, at least at some point in his life, many of the doctrines associated with his name. Pelagius was identified as an Irishman by Saint Jerome. Pelagius taught that the human will, as created with its abilities by God, was sufficient to live a sinless life, although he believed that Gods grace assisted every good work. Pelagianism has come to be identified with the view (whether taught by Pelagius or not) that human beings can earn salvation by their own efforts. Pelagius rejected the Augustinian concept of grace. According to his opponents, Pelagius taught that moral perfection was attainable in this life without the assistance of divine grace through human free will. Augustine contradicted this by saying that perfection was impossible ...

Another group with emphasis on perfectionism was Pelagianism. Pelagius was strongly influenced by Aristotelian and Stoic philosophy and ethics. He desired to lead the Church to monarchist, ascetic perfection Harnack, V. pp. 170ff). His concern for sinless perfection appears most strikingly in his letter to the noble virgin Demetrias. Pelagianism only became problematic and even heretical when Augustinianism became its forceful counter, which ultimately triumphed at the Council at Carthage (418 A.D.). Pelagius call to sinless perfection (amartetos) after baptism was not an innovation. Both Justin and Athanasius were Pelagians before Pelagius in this respect. The other critical Pelagian emphasis was justification sola fide. Augustine failed to perceive this Pelagian feature and only after the recovery of the of his Exposition of Thirteen Epistles of St. Paul in the 20th century (J.A. Robinson, 3 volumes 1922-1931 reprint 1967 and R.F. Evans, Four Letters of Pelagius, 1968, pp. 34ff. and his ...

Posted on 08/04/2004 12:32:44 PM PDT by xzins. The Life of St. Morgan of Wales. AKA Pelagius. Early Life and Education. St. Morgan of Wales is more commonly known by his Latin name Pelagius Britto -- indicating his association with the sea and Celtic British origins. He was born around 360 A.D. in South Wales in Bangor-is-y-coed or Caerlleon-ar-wsyg near the Severn estuary. He came from a Christian romanized Celtic background, the son of a decurion.. Morgan received a Latin education and was taught Holy Scriptures, inheriting the Celtic tradition which had links with the Church of Gaul and the Eastern Church. An emphasis was placed on faith and good works, on the holiness of all life, and on the oneness-of-all.. In 380 Morgan went to Rome to study law but soon abandoned his law career for the Church, becoming a monk. In doing so, he was to become the first-known major Celtic writer and theologian.. Morgan was a big, enthusiastic man -- strong, broad-shouldered and stout. His physical stature was ...

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Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified ...

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized - and confirmed - that co-cultivation under glucose as the sole carbon source would result in competitive interactions ...

Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of wax esters that are high-value materials and can be used in a variety of industrial applications. The products of WSs include fatty acid ethyl esters, which can be directly used as biodiesel. Here, heterologous WSs derived from five different organisms were successfully expressed and evaluated for their substrate preference in Saccharomyces cerevisiae. We investigated the potential of the different WSs for biodiesel (that is, fatty acid ethyl esters) production in S. cerevisiae. All investigated WSs, from Acinetobacter baylyi ADP1, Marinobacter hydrocarbonoclasticus DSM 8798, Rhodococcus opacus PD630, Mus musculus C57BL/6 and Psychrobacter arcticus 273-4, have different substrate specificities, but they can all lead to the formation of biodiesel. The best biodiesel producing strain was found to be

Pelagianism. There are many Calvinists who love to project the Arminianism/Calvinism debate back onto the debate between Augustine and Pelagius in the late 4th century. When doing so, Calvinism clearly goes to the Augustinian side since it really is the Protestant version of what Augustine taught on these matters. However, one has to be either grossly ignorant or malevolently deceitful in order to claim that Arminianism is akin to Pelagiuss views (Arminianism is in fact very similar to the semi-Augustinian view which arose in the early 5th century).. For those who are neither ignorant nor deceitful but still want to use the Augustine/Pelagius debate as a Calvinist claim to legitimacy, they resort to saying that Arminianism leads to Pelagian views. Usually, this claim is simply stated in such terms as on its way to Pelagianism or it is the first step towards Pelagianism. What I have never heard is any attempt to explain how a belief in Arminianism would actually lead to a belief in ...

We analyzed gabbroic and dioritic rocks from the Atud igneous complex in the Eastern Desert of Egypt to understand better the formation of juvenile continental crust of the Arabian-Nubian Shield. Our results show the rocks are the same age (U-Pb zircon ages of 694.5 ± 2.1 Ma for two diorites and 695.3 ± 3.4 Ma for one gabbronorite). These are partial melts of the mantle and related fractionates (εNd690 = +4.2 to +7.3, ⁸⁷Sr/⁸⁶Sri = 0.70246 to 0.70268, zircon δ¹⁸O ∼ +5 ‰). Trace element patterns indicate that Atud magmas formed above a subduction zone as part of a large and long-lived (∼60 Ma) convergent margin. Atud complex igneous rocks belong to a larger metagabbro-epidiorite-diorite complex that formed as a deep crustal mush into which new pulses of mafic magma were periodically emplaced, incorporated, and evolved. The petrologic evolution can be explained by fractional crystallization of mafic magma plus variable plagioclase accumulation in a mid- to lower crustal MASH ...

Polaris is a American Stout style beer brewed by Rising Tide Brewing Company in Portland, ME. 3.91 average with 100 ratings, reviews and opinions.

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Chinas demand for Fatty Alcohol has grown at a fast pace in the past decade. In the next decade, both production and demand will continue to grow. The

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Define Saybolt viscosity: viscosity as determined by the number of seconds required for an oil heated to 130° F for lighter oils and 210° F for…

Researchers analyzed bacterial communities exposed to Deepwater Horizon oil and identified taxa and genes associated with oil degradation and assimilation. The scientists found that Marinobacter and Alcanivorax dominated alkane-degrading communities, while Alteromonadales, Oceanospirillales, and Rhodospirillales dominated polycyclic aromatic hydrocarbon (PAH)-degrading communities. Oil-degrading bacteria exhibited genes associated with motility and nutrient uptake needed for nutrient-limiting conditions that are characteristic of oil-induced bacterial blooms. These results suggest that the degradation of oil associated with a spill requires the coordinated response of a complex bacterial community. The researchers published their findings in Nature Microbiology: Reconstructing metabolic pathways of hydrocarbon-degrading bacteria from the Deepwater Horizon oil spill.. Previous studies correlated observed shifts in microbial community structures with degradation and use of Deepwater Horizon ...

Macronutrients such as nitrogen (N), phosphorus (P), and silicon (Si) are essential for the productivity and distribution of diatoms in the ocean. Responses of diatoms to a particular macronutrient deficiency have been investigated, however, we know little about their common or specific responses to different macronutrients. Here, we investigated the physiology and quantitative proteomics of a diatom Thalassiosira pseudonana grown in nutrient-replete, N-, P-, and Si-deficient conditions. Cell growth was ceased in all macronutrient deficient conditions while cell volume and cellular C content under P- and Si-deficiencies increased. Contents of chlorophyll a, protein and cellular N decreased in both N- and P-deficient cells but chlorophyll a and cellular N increased in the Si-deficient cells. Cellular P content increased under N-and Si-deficiencies. Proteins involved in carbon fixation and photorespiration were down-regulated under all macronutrient deficiencies while neutral lipid synthesis and ...

The global fatty alcohols market features a largely consolidated landscape, which in the coming years will increasingly shape the competitive dynamics of the market, notes Transparency Market Research (TMR). Prominent players in the global fatty alcohols market consider expanding their product portfolios as a key strategy to consolidate their positions.. Several of them are also focusing on expanding their regional footprints in order to get stronghold over the global fatty alcohols market. In particular, numerous chemical manufacturers are shifting their attention to new revenue streams in Asia Pacific where, they intend to gain from the availability of cheap raw materials, observes TMR.. Leading players in the global fatty alcohols market are VVF LLC, Kuala Lumpur Kepong Berhad (KLK), Musim Mas Holdings, Wilmar International Ltd., and Sasol Limited.. The global fatty alcohols market is forecast to rise at a CAGR of 5.1% during 2015-2023. By the end of this period, the global market is ...

Dissolution of the frustule in water is minimized because it is surrounded by an organic casing that includes glycoproteins with high levels of rhamnose and xylose, two sugars rarely found in other eukaryotic glycans. Based on the presence of highly conserved calcium-binding domains (30), four new frustulins (casing glycoproteins) were identified, but no pleuralins, which are associated with terminal girdle bands in C. fusiformis (31). Consistent with a high content of hydroxylated amino acids found in the organic casing (32), one of the more abundant gene families in T. pseudonana encodes prolyl-4 hydroxylases.. Staying afloat. Diatoms must compensate for their dense silica frustule to maintain position within the illuminated portion of the water column. Some diatoms, including members of Thalassiosira, apparently increase drag and thus decrease sinking rates by extruding chitin fibers from pores in the frustule (24). These fibers can represent as much as 40% of total cell biomass and 20% of ...

Dissolution of the frustule in water is minimized because it is surrounded by an organic casing that includes glycoproteins with high levels of rhamnose and xylose, two sugars rarely found in other eukaryotic glycans. Based on the presence of highly conserved calcium-binding domains (30), four new frustulins (casing glycoproteins) were identified, but no pleuralins, which are associated with terminal girdle bands in C. fusiformis (31). Consistent with a high content of hydroxylated amino acids found in the organic casing (32), one of the more abundant gene families in T. pseudonana encodes prolyl-4 hydroxylases.. Staying afloat. Diatoms must compensate for their dense silica frustule to maintain position within the illuminated portion of the water column. Some diatoms, including members of Thalassiosira, apparently increase drag and thus decrease sinking rates by extruding chitin fibers from pores in the frustule (24). These fibers can represent as much as 40% of total cell biomass and 20% of ...

The effect of L. anguillarum and V. splendidus on the bacterial community associated with rotifers and seawater of rearing tanks was analysed by DGGE of PCR-amplified 16S rDNA fragments. The bacterial community of rotifers did not present a marked species dominance. The incorporation of L. anguillarum or V. splendidus did not reduce bacterial diversity and shifts could be explained by bacterial exchange between rotifers and seawater. Main bacterial groups were identified by sequencing the DNA extracted from Marine Agar (MA) bacterial isolates and DGGE excised bands. Only 2 DGGE bands corresponded to bacteria isolated from MA plates, suggesting that bacterial groups present in rotifers may not be easily cultivable. The bacterial community of rotifers was composed by Gram negative bacteria belonging to α-Proteobacteria (Ruegeria spp), γ-Proteobacteria (Alteromonas alvinellae, Marinobacter sp, an Oceanospirillaceae bacterium and Pseudoalteromonas sp), Cytophaga-Flexibacter-Bacteroides group ...

About Fatty Alcohols Market Fatty alcohols are alcohols derived from natural fats and oils present in plants and animals. These are derived from fatty acids and ...

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Indonesia will appeal the WorldTrade Organizations ruling in favour of European Unionanti-dumping duties on the export of certain fatty alcohols fromthe country, the trade ministry said on Friday.

Combination of chemical and electron-impact ionisation with GC x GC-qMS for characterization of fatty alcohol alkoxylate polymers in the low-molecular-weight range up to 700 ...

Shea C, Clauwaert P, Verstraete W, Nerenberg R. 2008. Adapting a denitrifying biocathode for perchlorate reduction. Water, Science and Technology. 58(10):1941-1946. ...

Shea C, Clauwaert P, Verstraete W, Nerenberg R. 2008. Adapting a denitrifying biocathode for perchlorate reduction. Water, Science and Technology. 58(10):1941-1946. ...

Knowing that dimethiconols or silanols condense under acid or alkaline catalysis, a study was undertaken to determine if these compounds could be successfully ethoxylated under conditions similar to...