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The RAS/RAF/MEK pathway is activated in more than 30% of human cancers, most commonly via mutation in the K-ras oncogene and also via mutations in BRAF. Several allosteric mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors, aimed at treating tumors with RAS/RAF pathway alterations, are in clinical development. However, acquired resistance to these inhibitors has been documented both in preclinical and clinical samples. To identify strategies to overcome this resistance, we have derived three independent MEK inhibitor-resistant cell lines. Resistance to allosteric MEK inhibitors in these cell lines was consistently linked to acquired mutations in the allosteric binding pocket of MEK. In one cell line, concurrent amplification of mutant K-ras was observed in conjunction with MEK allosteric pocket mutations. Clonal analysis showed that both resistance mechanisms occur in the same cell and contribute to enhanced resistance. Importantly, in all cases the MEK-resistant ...
In recent years numerous agents targeting the Ras/Raf/MEK or PI3K/AKT/mTOR pathways have entered clinical development, and dual targeting of these pathways is now been investigated clinically. It had been generally accepted, and shown, that direct inhibition of targets subject to mutational activation is effective (42), and this expectation has to some extent translated to cases in which the mutationally activated pathway is targeted at a nonmutated node (e.g., MEK inhibitors and Rapalogues). However, in some settings these initial observations have been shown to be more complicated, and the dependency of cancer cells for survival on both of these pathways is becoming better understood (25, 27, 43). In this study we report that dual targeting of the MEK and mTOR pathways, using well-tolerated schedules of the inhibitors selumetinib and AZD8055, respectively, results in enhanced antitumor efficacy across a panel of NSCLC and CRC xenograft and patient-derived explant models. Furthermore, ...
Selumetinib (AZD6244) 是一种高效选择性的,非 ATP 竞争性的 MEK1/2 抑制剂, 抑制 MEK1 的 IC50 为 14 nM。Selumetinib (AZD6244) 抑制 MEK1/2 磷酸化水平。- 高纯度,全球文献引用。
Selumetinib (AZD6244)是一种有效,高选择性的MEK1抑制剂,无细胞试验中IC50为14 nM,也抑制ERK1/2磷酸化,IC50为10 nM,对p38α, MKK6, EGFR, ErbB2, ERK2, B-Raf等没有抑制作用。Phase 3。
MEK3兔多克隆抗体(ab5428)可与小鼠, 人样本反应并经WB, ELISA, IHC实验严格验证,被4篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
MEK1小鼠单克隆抗体[32G3](ab70833)可与小鼠, 大鼠, 人样本反应并经WB, ELISA实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
On the basis of its MEK inhibitory activity, E6201 was shown here to be an ATP-competitive MEK inhibitor that is effective against BRAF-V600E melanoma in a preclinical setting. Furthermore, we showed that E6201 is effective in a preclinical model against BRAF-V600E melanoma harboring the MEK1-C121S mutation. Almost all of the MEK inhibitors previously reported are allosteric inhibitors, not ATP-competitive inhibitors (37). Our docking simulation showed that E6201 and selumetinib bind to different sites when they dock with MEK. These results suggest that the inhibitory mode of action of E6201 is different from that of allosteric MEK inhibitors and that E6201 merits further investigation in a clinical setting against both MEK-WT melanomas and melanomas harboring MEK with mutations at the allosteric site.. The MEK1-C121S mutation, which confers resistance to vemurafenib, increases MEK activity independently from BRAF and also confers resistance to the allosteric MEK inhibitor selumetinib (12). Our ...
Treatment with selumetinib benefits women with tumors overexpressing p-ERK. Low-grade serous ovarian cancer is notoriously resistant to cytotoxic chemotherapy; fewer than 15% of women respond initially. But in a phase II trial of the experimental MAP-ERK kinase (MEK) inhibitor selumetinib (AZD6244), women who had failed 3 or more rounds of chemotherapy had 81% disease control and a median progression-free survival (PFS) of 11.3 months.. Unexpectedly, benefit correlated more closely with the protein p-ERK than with mutation of BRAF, the protein that selumetinib targets.. About 60% of low-grade ovarian tumors have RAS/RAF activation of the MAPK pathway. However, for this trial, the researchers did not have exhaustive mutational analysis prior to selecting the 52 participants.. "In this era of individualized medicine, we still enrolled participants regardless of tumor genotype, and were glad we did," says John Farley, MD, a professor at Creighton University School of Medicine at St. Josephs ...
Purpose: To assess the role of Apollon in melanoma resistance to intrinsic and extrinsic pathways of apoptosis and to identify strategies to reduce its expression.. Experimental Design: Apollon expression was assessed in melanoma cells in vitro and in vivo. Apollon modulation and melanoma apoptosis were evaluated by Western blot and/or flow cytometry in response to cytotoxic drugs, mitogen-activated protein/extracellular signal-regulated kinase (MEK)-, BRAFV600E-, and mTOR-specific inhibitors, TRAIL and anti-HLA class II monoclonal antibodies (mAb). Mitochondrial depolarization, caspase activation, apoptosis assays, and gene expression profiling were used to test effects of Apollon silencing, by siRNA, on melanoma response to antitumor agents.. Results: Apollon was constitutively expressed by melanoma cells, in vitro and in vivo, and at higher levels than in benign melanocytic lesions. Melanoma apoptosis correlated significantly with Apollon protein downmodulation in response to cytotoxic drugs, ...
Array BioPharma announced results of a Phase 2 trial of selumetinib in women with recurrent low-grade serous ovarian or peritoneal cancer.
This is a Phase 1 study during which patients with advanced cancer will receive investigational study drug ARRY-382. Patients will receive increasing do
Two techniques have been utilized to make sure higher precision of the results. The data have been 1st manually transferred to standardized forms, then double
Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20-25% fermentable sugars and bioethanol fermentations at 13% fermentable sugars. RCK2 encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress, RCK2 expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an rck2 null strain. A strain overexpressing RCK2 had significantly faster fermentation rates when compared
This study looks at the side effects and best dose of trametinib (targeted chemotherapy) in treating patients with cancer that has spread to other places in the body and usually cannot be cured or controlled with treatment (advanced) with or without liver (hepatic) dysfunction. Trametinib may stop the growth of tumor cells by blocking proteins needed for cell growth. When these proteins are blocked, the growth of cancer cells may be stopped and the cancer cells will then die. Liver dysfunction is frequently found in patients with advanced cancer and usually prevents patients from receiving standard treatments or from participating in clinical trials. Patients may also need dose adjustments or absorb drugs differently. Trametinib may be a better treatment for patients with advanced cancers and liver dysfunction. ...
This phase I trial studies the side effects and best dose of cediranib maleate and selumetinib in treating patients with solid malignancies. Cediranib m
Phase III results were reported separately for GlaxoSmithKlines BRAF inhibitor dabrafenib, and MEK inhibitor trametinib as monotherapies for the treatment of BRAF mutation-positive
The goal of this clinical research study is to learn the highest tolerable dose of ARRY-614 that can be given to patients with MDS either 1 or 2 times a day. ARRY-614 is designed to block the function of certain proteins that cause normal blood cells to die in patients with MDS. This could cause the normal blood cells to survive.
U.S., March 2 -- ClinicalTrials.gov registry received information related to the study (NCT03065387) titled Study of the Pan-ERBB Inhibitor Neratinib Given in Combination With Everolimus, Palbociclib or Trametinib in Advanced Cancer Subjects With EGFR Mutation/Amplification, HER2 Mutation/Amplification or HER3/4 Mutation on Feb. 22. Brief Summary: There are 2 parts to this clinical research study: Part 1 (dose escalation) and Part 2 (expansion). The goal of Part 1 of this study is to find the highest tolerable dose of neratinib in combination with everolimus, Ibrance (palbociclib), or trametinib that can be given to patients who have advanced cancer with a specific mutation (EGFR, HER2, HER3, or HER4). The goal of Part 2 of this study is to learn if the dose of neratinib in combination with everolimus, Ibrance (palbociclib), or trametinib found in Part 1 can help to control advanced cancer in patients who have a specific mutation. The safety of this drug will also be studied in both parts of ...
The kinases MEK1 and MEK2 have the same substrates, ERK1 and ERK2, but mice lacking Mek1 die as embryos due to placental defects and mice lacking Mek2 are viable, implying isoform-specific functions. Aoidi et al. determined that MEK1 and MEK2 are functionally redundant as long as sufficient protein is produced. Mice lacking Mek1 were rescued by placing the coding sequence of Mek2 into both alleles of Mek1, but placental defects and embryonic lethality occurred when the mice carried only one copy of this knock-in allele along with a null allele of Mek1. The data indicated that the proteins functionally substituted for one another, but that the developing placenta is particularly sensitive to the amount of MEK present and producing the minimum amount required at least four copies of Mek2 in the absence of Mek1 or two copies of Mek1 in the absence of Mek2. Thus, the products may be functionally identical, but differences in their expression, translation, and protein half-life enable ...
The FDA has granted an accelerated approval to the combination of the MEK inhibitor trametinib and the BRAF inhibitor dabrafenib as a treatment for patients with unresectable or metastatic melanoma that harbors a BRAF V600E or V600K mutation.
This study is investigating the efficacy of dabrafenib + trametinib in patients with BRAF mutation-positive melanoma that has metastasized to the brain. Four
Such any conclusion is contract with prior scientific studies showing which eCBs work as retrograde messengers in glutamate synapses of putative Doctor 5-HT neurons (Haj-Dahmane & Shen, June 2006, 09).
The mammalian genome contains two mitogen-activated protein kinase (MAPK) kinase (MEK)-encoding genes, Mek1 and Mek2. MEKs phosphorylate and activate the two extracellular signal-regulated kinase (ERK) isoforms ERK1 and ERK2. Mek1−/− embryos die due to placental defects, whereas Mek2−/− mice survive with a normal life span and fertility, suggesting that MEK1 has functions not shared by MEK2. However, most Mek1+/−Mek2+/− embryos also die from placental defects, indicating that both Mek genes contribute to placental development. To assess the functional specificity of the Mek1 and Mek2 genes, we produced a Mek1 knock-in allele in which the Mek2 coding sequences were placed under the control of Mek1 regulatory sequences (Mek12 allele). Mek12/2 mice were viable with no apparent phenotype, indicating rescue by MEK2 and functional redundancy between the two MEK proteins. However, Mek12/− embryos with Mek2 in only one of the Mek1 alleles and the other Mek1 allele null died from abnormal ...
Currently, there are no available targeted therapy options for non-V600 BRAF mutated tumors. The aim of this study was to investigate the effects of RAF and MEK concurrent inhibition on tumor growth, migration, signaling and apoptosis induction in preclinical models of non-V600 BRAF mutant tumor cell lines. Six BRAF mutated human tumor cell lines CRL5885 (G466 V), WM3629 (D594G), WM3670 (G469E), MDAMB231 (G464 V), CRL5922 (L597 V) and A375 (V600E as control) were investigated. Pan-RAF inhibitor (sorafenib or AZ628) and MEK inhibitor (selumetinib) or their combination were used in in vitro viability, video microscopy, immunoblot, cell cycle and TUNEL assays. The in vivo effects of the drugs were assessed in an orthotopic NSG mouse breast cancer model. All cell lines showed a significant growth inhibition with synergism in the sorafenib/AZ628 and selumetinib combination. Combination treatment resulted in higher Erk1/2 inhibition and in increased induction of apoptosis when compared to single agent
The official website for TAFINLAR® (dabrafenib) capsules and MEKINIST® (trametinib) tablets. Find information about TAFINLAR + MEKINIST, the conditions it treats, and Important Safety Information.
TY - JOUR. T1 - Targeting protein translation in human non-small cell lung cancer via combined MEK and mammalian target of rapamycin suppression. AU - Legrier, Marie Emmanuelle. AU - Yang, Chia Ping Huang. AU - Yan, Han Guang. AU - Lopez-Barcons, Lluis. AU - Keller, Steven M.. AU - Pérez-Soler, Roman. AU - Horwitz, Susan Band. AU - McDaid, Hayley M.. PY - 2007/12/1. Y1 - 2007/12/1. N2 - Lung cancer is a genetically heterogeneous disease characterized by the acquisition of somatic mutations in numerous protein kinases, including components of the rat sarcoma viral oncogene homolog (RAS) and AKT signaling cascades. These pathways intersect at various points, rendering this network highly redundant and suggesting that combined mitogen-activated protein/extracellular signal-regulated kinase (MEK) and mammalian target of rapamycin (mTOR) inhibition may be a promising drug combination that can overcome its intrinsic plasticity. The MEK inhibitors, CI-1040 or PD0325901, in combination with the mTOR ...
Learn all about dabrafenib (Tafinlar) and trametinib (Mekinist) as separate drugs. Trametinib is a targeted therapy that targets the MEK 1 and MEK 2 protei. CancerWORLD Cancer Treatment Research Journal
PRIMARY OBJECTIVES:. I. To identify markers of intrinsic resistance to v-Raf murine sarcoma viral oncogene homolog B1 (B-RAF) targeted therapy in B-RAF mutation-positive melanoma.. SECONDARY OBJECTIVES:. I. To determine if intrinsic resistance can be reversed by mitogen activated protein kinase (MEK) targeted therapy and to identify biomarkers that correlate with this response.. II. To evaluate the feasibility of pre-surgical targeted therapy and serial tumor biopsies in patients with advanced, operable melanoma to determine if this model can be used to evaluate novel combinations of molecular targeted therapy in the future.. TERTIARY OBJECTIVES:. I. To determine if pre-surgical B-RAF and MEK targeted therapy is active and well tolerated in patients with advanced, operable melanoma. These findings may be used to support clinical trials in un-resectable, B-RAF mutation-positive melanoma.. OUTLINE:. Patients receive dabrafenib orally (PO) twice daily (BID) on days 1-28 adding trametinib on days ...
Compared with docetaxel, combination dabrafenib and trametinib was associated with improved PFS and OS in previously treated NSCLC patients.
... stay understood incompletely. versions mimicking MRD and relapse in sufferers. Surrogate and Principal MRD cells distributed main commonalities in reflection dating profiles, showing the suitability of our model. MRD cells uncovered main useful plasticity in?vivo and treatment level of resistance was reversible; MRD cells became delicate toward treatment once released from their in?vivo environment. Effective restorative strategies might goal at dissociating continual cells from their protecting market to prevent relapse in ALL individuals. Intro Relapse signifies a main danger for individuals with malignancy. After successful treatment initially, Trametinib uncommon growth cells might survive and re-initiate the cancerous disease with disappointing end result. Extreme lymphoblastic leukemia (ALL) is definitely connected with poor Trametinib diagnosis in babies and adult individuals and is definitely the most ...
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This phase I trial studies the side effects and best dose of trametinib with or without whole brain radiation therapy in treating patients with brain metastases. Trametinib may stop the growth of tumor cells by blocking some of the enzymes needed for cell growth. Radiation therapy uses high energy x rays to kill tumor cells. Drugs, such as trametinib, may make tumor cells more sensitive to radiation therapy. Giving trametinib with whole brain radiation therapy may be an effective treatment for brain metastases.
In the Clinic provides overviews of novel oncology agents, addressing indications, mechanisms, administration recommendations, safety profiles, and other essential information needed for the appropriate clinical use of these drugs.. Indication. On May 29, 2013, trametinib (Mekinist) was approved by the U.S. Food and Drug Administration (FDA) for treatment of patients with unresectable or metastatic melanoma with BRAF V600E or V600K mutation, as detected by an FDA-approved test.1,2 Concurrent with this approval, FDA approved the THxID BRAF assay (bioMérieux, Inc) for detection of BRAF V600E and V600K mutations. Trametinib is not indicated for treatment of patients who have received prior BRAF inhibitor therapy.. Pivotal Trial. Approval was based on improved progression-free survival in an international open-label trial in 322 patients with histologically confirmed stage IIIC or IV melanoma determined to be BRAF V600E or V600K mutation-positive based on centralized testing.3 Patients were ...
PRIMARY OBJECTIVES:. I. To ascertain the objective response rate (complete response and partial response) in patients with locally advanced or metastatic hepatocellular carcinoma treated with AZD6244 (selumetinib).. SECONDARY OBJECTIVES:. I. To assess the safety and tolerability of AZD6244 when administered to patients with hepatocellular carcinoma and mild (Childs A to compensated Childs B) liver dysfunction.. II. To describe the pharmacokinetics (PK) of AZD6244 in this patient population and compare in exploratory fashion to the established PK profile in patients with normal hepatic function.. III. To estimate the time to event functions of progression, progression-free survival (PFS), (and PFS associated with treatment), and overall survival.. IV. To explore, preliminarily, the possible correlations between baseline mitogen-activated protein kinase (MEK) activation (i.e., presence of phospho-MEK) and radiographic response or time to progression.. V. To investigate the effects of AZD6244 on ...
The MAP2K2 gene is associated with autosomal dominant Noonan syndrome (MedGen UID: 22527) and cardio-facio-cutaneous (CFC) syndrome (MedGen UID: 501103).
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