TY - JOUR. T1 - Intracellular transport of membrane glycoproteins. T2 - Two closely related histocompatibility antigens differ in their rates of transit to the cell surface. AU - Williams, D. B.. AU - Swiedler, S. J.. AU - Hart, Gerald Warren. PY - 1985. Y1 - 1985. N2 - The intracellular transport of two closely related membrane glycoproteins was studied in the murine B cell lymphoma line, AKTB-1b. Using pulse-chase radiolabeling, the kinetics of appearance of the class I histocompatibility antigens, H-2K(k) and H-2D(k), at the cell surface were compared and found to be remarkably different. Newly synthesized H-2K(k) is transported rapidly such that all radiolabeled molecules reach the surface within 1 h. In contrast, the H-2D(k) antigen is transported slowly with a half-time of 4-5 h. The rates of surface appearance for the two antigens closely resemble the rates at which their Asn-linked oligosaccharides mature from endoglucosaminidase H (endo-H)-sensitive to endo H-resistant forms, a process ...

時間に依存するファジィ集合族を用いた最適制御に関する一考察 (函数解析学の応用としての情報数理の研究) （2001 ...

114) 多数の重症合併症を併発するも救命し得た急性感染性心内膜炎の一例(第189回日本循環器学会関東甲信越地方会) （2004 ...

Staphylococcus aureus; pan ID: SAUPAN004467000; products: autolysin, N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoprotein endo-beta-N-acetylglucosamidase, beta-N-acetylglucosaminidase, putative, exported protein, family 4 N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoendo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-glucosaminidase, peptidoglycan endo-beta-N-acetylglucosaminidase

Ethanol production from rice husk by simultaneous saccharification and fermentation using Mucor hiemalis was investigated. To reach the maximum ethanol production yield, the most important influencing factors in the pretreatment process, including temperature (0-100°C), NaOH concentration (1-3 M), and the pretreatment time (30-180 min), were optimized using an experimental design by a response surface methodology (RSM). The maximum ethanol production yield of 86.7 % was obtained after fungal cultivation on the husk pretreated with 2.6 M NaOH at 67°C for 150 min. This was higher than the yield of 57.7% obtained using Saccharomyces cerevisiae as control. Furthermore, fermentation using M. hiemalis under the optimum conditions led to the production of a highly valuable fungal biomass, containing 60 g glucosamine (GlcN), 410 g protein, and 160 g fungal oil per each kg of the fungal biomass.

Tarentino AL, Maley F. 1975. A comparison of the substrate specificities of endo-beta-N-acetylglucosaminidases from Streptomyces griseus and Diplococcus Pneumoniae.. Biochem Biophys Res Commun. 67(1):455-62. ...

Shop Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase ELISA Kit, Recombinant Protein and Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

1M03: Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.

1M03: Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.

The optimal pH for the cleavage of fucosylated-biantennary (i.e. porcine fibrinogen) and triantennary glycans (i.e. fetuin) by Endo F3 is pH 4.5 (10X Glycobuffer 4).

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K01186 NEU1; sialidase-1 [EC:3.2.1.18] K01186 NEU1; sialidase-1 [EC:3.2.1.18] K01190 lacZ; beta-galactosidase [EC:3.2.1.23] K12373 HEXA_B; hexosaminidase [EC:3.2.1.52] K12373 HEXA_B; hexosaminidase [EC:3.2.1.52] K01191 MAN2C1; alpha-mannosidase [EC:3.2.1.24] K01227 E3.2.1.96; mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [EC:3.2.1.96] K01206 FUCA; alpha-L-fucosidase [EC:3.2.1.51] K15923 AXY8; alpha-L-fucosidase 2 [EC:3.2.1.51 ...

To further assess the subcellular localization of the ER-retained mutants, Endo H sensitivity and resistance in vitro assays on the expressed proteins were carried out. Frizzled family receptor 4 protein has two potential N-glycosylation sites in its extracellular domain (Fig. 1, arrows). The principle of this assay is that the carbohydrate moieties of the ER-localized glycoproteins are cleavable by Endo H, whereas the post-ER species are resistant due because of further remodeling of their N-glycans in the Golgi complex. Endo H cleaves after the first asparagine-linked N-acetylglucosamine (N-glycan) of high-mannose and hybrid polysaccharides. Therefore, one can expect that the mutants retained in the ER will have their N-glycans cleaved when treated with Endo H. Proteins that have reached the Golgi complex would be resistant to Endo H treatment. Upon Endo H treatment, P33S, G36N, H69Y, M105V, M105T, C204Y, and C204R mutants showed complete conversion of the high-molecular-weight band into a ...

Nearly all proteins entering the lumen of the endoplasmic reticulum (ER) become glycosylated en route to a cellular organelle, the plasma membrane, or the extracellular space

Nearly all proteins entering the lumen of the endoplasmic reticulum (ER) become glycosylated en route to a cellular organelle, the plasma membrane, or the extracellular space

The intracellular fates of membrane and secretory immunoglobulin heavy chains were examined in a pre-B cell line that has switched to the gamma isotype. The membrane form of the heavy chain (gamma m) was rapidly degraded while the secretory form (gamma s) was retained intracellularly in association with BiP. The degradation of gamma m could not be inhibited by ammonium chloride, chloroquine, or monensin suggesting that it occurred in a nonlysosomal compartment. The inability to detect any Endo H-resistant form of gamma m before its degradation suggested that degradation occurs before entry into the Golgi compartment. Degradation of gamma m could be inhibited by incubation at 24 degrees C. In a derivative of this cell line expressing a transfected kappa gene, gamma s formed disulfide linked tetramers with kappa and was secreted, while gamma m, although associated with kappa, continued to be rapidly degraded. These observations suggest that membrane and secretory heavy chain proteins are retained ...

Govaerts, R. et al. 2016. Caladenia hiemalis in World Checklist of Selected Plant Families. The Board of Trustees of the Royal Botanic Gardens, Kew. Published on the internet. Accessed: 2016 July 31. Reference page ...

keratanase II: an endo-beta-N-acetylglucosaminidase; cleaves the beta(1-3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine

At moderate levels (0.5-1.0% ionic and non-ionic detergents) many of the glycosidases show satisfactory activity or are unaffected. Major exceptions include PNGase F and |em|O|/em|-Glycosidase, which are inhibited by SDS.

全著者名】 K. Kosaka / K. Uozumi / A. Nakada / H. Kubota / T. Ohmi / T. Iwabuchi / T. Baba / T. Endo / H. Hashiguchi / H. Furukawa / Y. Egashira / HASHIMOTO SEIJI / M. ...

Highlights of ENDO, Highlights of ENDO: Argentina is a Medical Conference, organized in Córdoba, Argentina. Learn More About Event

My doc is sending me for an MRI to see what is going on in my abdomen, endo tissue is pushing into bowel and causing blockage. I thought endo tissue was only...

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins. - Find MSDS or SDS, a COA, data sheets and more information.

Find Solenia Pink Begonia (Begonia x hiemalis Solenia Pink) in Orange County, CA California CA at Rogers Gardens (Hiemalis Begonia)

The CD109 antigen is a monomeric glycosyl phosphatidylinositol (GPI)-linked glycoprotein of 170 kDa that contains several N-linked endoglycosidase H-sensitive hybrid-type glycans but no O-linked glycan. It has been reported as a novel member of the α2 macroglobulin (α2M) / C3, C4, C5 family of thioester-containing proteins. The CD109 antigen is found on vascular endothelial cells, some epithelial cells, activated, but not resting, T-cells, activated, but not resting, platelets, leukemic megakaryoblasts and a subset of bone marrow CD34+ cells. This antigen is not expressed on fresh peripheral blood lymphocytes (PBL). Poorly differentiated (CD34+, TdT+, CD7+) T-acute leukemias and rare cases of chronic myeloid leukemia in megakaryoblast crisis express the CD109 antigen. Furthermore, megakaryoblastoid cell lines (MO7e, MOLM-1) are CD109+. The CD109 antigen, strongly expressed on KG1a cell line with 20,000 binding sites per cell, may represent a very early marker for hematopoietic cells committed ...

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TY - JOUR. T1 - Human herpesvirus-8 glycoprotein B interacts with Epstein-Barr virus (EBV) glycoprotein 110 but fails to complement the infectivity of EBV mutants. AU - Pertel, Peter E.. AU - Spear, Patricia G.. AU - Longnecker, Richard. PY - 1998/11/25. Y1 - 1998/11/25. N2 - To characterize human herpesvirus 8 (HHV-8) gB, the open reading frame was PCR amplified from the HHV-8-infected cell line BCBL-1 and cloned into an expression vector. To facilitate detection of expressed HHV-8 gB, the cytoplasmic tail of the glycoprotein was tagged with the influenza hemagglutinin (HA) epitope. Expression of tagged HHV-8 gB (gB-HA), as well as the untagged form, was readily detected in CHO-K1 cells and several lymphoblastoid cell lines (LCLs). HHV-8 gB-HA was sensitive to endoglycosidase H treatment, and immunofluorescence revealed that HHV-8 gB- HA was detectable in the perinuclear region of CHO-K1 cells. These observations suggest that HHV-8 gB is not processed in the Golgi and localizes to the ...

The study of bacterial glycosidases has emerged as a field at the intersection of microbial pathogenesis and glycobiology. By studying the mechanisms by which bacteria interfere with host glycosylation, new insight can be gained into both bacterial pathogenesis and the impact of glycosylation of the immune system. Interfering with the glycosylation of the host defence is widespread among pathogenic bacteria for modulation of the functions of the immune system or as a way of utilizing the glycans of glycoproteins as nutrients [24,33].. For example, Enterococcus faecalis, a Gram-positive gut bacterium and opportunist, secretes EndoE, an endoglycosidase with activity on the Fc-glycan on IgG and on the glycoprotein RNase B that promotes bacterial growth when nutrients are scarce [24,34]. The endoglycosidases EndoF1-3 from E. meningoseptica and EndoH from Streptomyces plicatus has been shown to be glycan-specific: high-mannose and hybrid oligosaccharides are cleaved by EndoF1 and EndoH, whereas ...

Press Release issued Aug 16, 2016: The report provides comprehensive information on the Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50), targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. The report also covers the descriptive pharmacological action of the therapeutics, its complete research and development history and latest news and press releases. Additionally, the report provides an overview of key players involved in Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) targeted therapeutics development and features dormant and discontinued projects.

For more information on Glycobiology please browse our online Glycobiology Analysis Manual.

TY - JOUR. T1 - Modeling of the N-Glycosylated Transferrin Receptor Suggests How Transferrin Binding Can Occur within the Surface Coat of Trypanosoma brucei. AU - Mehlert, Angela. AU - Wormald, Mark R.. AU - Ferguson, Michael A. J.. PY - 2012/4. Y1 - 2012/4. N2 - The transferrin receptor of bloodstream form Trypanosoma brucei is a heterodimer encoded by expression site associated genes 6 and 7. This low-abundance glycoprotein with a single glycosylphosphatidylinositol membrane anchor and eight potential N-glycosylation sites is located in the flagellar pocket. The receptor is essential for the parasite, providing its only source of iron by scavenging host transferrin from the bloodstream. Here, we demonstrate that both receptor subunits contain endoglycosidase H-sensitive and endoglycosidase H-resistant N-glycans. Lectin blotting of the purified receptor and structural analysis of the released N-glycans revealed oligomannose and paucimannose structures but, contrary to previous suggestions, no ...

We studied the effect of the plant alkaloid castanospermine on the biosynthesis and secretion of human hepatoma glycoproteins. The HepG-2 cells, grown in the presence or absence of the alkaloid, were labelled with [2-3H]mannose and then the labelled glycopeptides were prepared by Pronase digestion. This material was analysed by gel filtration on Bio-Gel P-4 before and after treatment with endo-beta-N-acetylglucosaminidase H. Castanospermine caused an accumulation of high-mannose oligosaccharides, by 70-75% over control. The major accumulated product, which could also be labelled with [3H]galactose and was only partially susceptible to alpha-mannosidase digestion, was identified by h.p.l.c. as a Glc3Man9GlcNAc. Thus the alkaloid inhibits glucosidase I in the human hepatoma cells. Analysis of total glycoproteins secreted by the cells into the medium revealed the presence of only complex oligosaccharides in both control and treated cultures, and the amount of the oligosaccharides labelled with ...

The cDNA encoding a xyloglucan endotransglycosylase, PttXET16A, from hybrid aspen (Populus tremula×tremuloides) has been isolated from an expressed sequence tag library and expressed in the methylotrophic yeast Pichia pastoris. Sequence analysis indicated a high degree of similarity with other proteins in the XTH (xyloglucan transglycosylase/hydrolase) gene subfamily of GH16 (glycoside hydrolase family 16). In addition to the conserved GH16 catalytic sequence motif, PttXET16A contains a conserved N-glycosylation site situated proximal to the predicted catalytic residues. MS analysis indicated that the recombinant PttXET16A expressed in P. pastoris is heterogeneous due to the presence of variable N-glycosylation and incomplete cleavage of the α-factor secretion signal peptide. Removal of the N-glycan by endoglycosidase H treatment did not influence the catalytic activity significantly. Similarly, site-directed mutagenesis of Asn93 to serine to remove the N-glycosylation site resulted in an ...

Plicamycin (INN, also known as mithramycin; trade name Mithracin) is an antineoplastic antibiotic produced by Streptomyces plicatus. It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently reported in different places all with the same chromomycin core, but with different stereochemistry in the glycoside chain, a 1999 study has re-investigated the compound and proposed a revised structure. Plicamycin has been used in the treatment of testicular cancer, Pagets disease of bone, and, rarely, the management of hypercalcemia. Plicamycin has been tested in chronic myeloid leukemia. Plicamycin is currently used in multiple areas of research, including cancer cell apoptosis and as a metastasis inhibitor. One elucidated pathway shows it interacts by cross-binding chromatin GC-rich promoter motifs, thereby inhibiting gene transcription. Mithramycin A. Fermentek. Wohlert, S. E.; Künzel, E.; Machinek, R.; Méndez, C.; Salas, J. A.; ...

Published reports disagree on the best features for detecting and distinguishing between Flavobacterium meningosepticum (biovar IIa) and Flavobacterium species CDC group IIb (biovar IIb; Flavobacterium indologenes). This report discloses that at least some of these disagreements may reflect the methods used. To detect production of indole, a modified Kovács reagent (not Ehrlich) and a buffered tryptophan medium were optimal, but not all strains of these two biovars produced indole. To distinguish the two biovars, hydrolysis of corn starch was preferable to that of soluble potato starch. Both biovars may hydrolyze DNA; the differentiation achieved varied with the methods used. Both biovars presented pigmented growth; only IIb, however, was obviously pigmented on a 2-day blood agar plate. Acidification of D-arabinose definitively distinguished these two biovars; several additional features were useful but not definitive. ...

Chryseobacterium meningosepticum is a Gram-negative rod with a worldwide distribution. A case of C. meningosepticum cellulitis with severe sepsis and hepatitis in a 36-year-old male patient is described.

Asteatotic dermatitis eczema craquel eczema craquelatum xerotic eczema eczema hiemalis eczema fendille etat craquel Pruritic, cracked, and fissured skin occurring most commonly on the shins of elderly patients, caused by lack of moisture in the skin Physiologic process with aging seen more often in the winter, with cold air outside and heated air inside causing a decrease in humidity loss of water by stratum cor-neum causing cells to shrink and creating fine fissures eczematous changes.... ...

Poly(2-oxazoline)s (POx) are innovative alternatives to PEGs. Poly(2-oxazoline)s are biocompatible, nontoxic, antimicrobial, antifungal and antifouling.

Study Endo p. 322-326 flashcards from Davinchi M 's UMMS class online, or in Brainscape's iPhone or Android app. ✓ Learn faster with spaced repetition.

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The gene encoding the major envelope glycoprotein complex, gp55-116 (gB), of human cytomegalovirus (HCMV) was expressed at high levels in insect cells utilizing a recombinant baculovirus. The mature intracellular form of the insect-derived gp55-116 was a protein of M r 150K which contained approximately 50K of N-linked oligosaccharides. The oligosaccharide linkages were almost exclusively endoglycosidase H-sensitive. The 150K protein was processed, presumably by proteolytic cleavage, to yield at least one of the previously defined cleavage products of the gp55-116. This processing step was significantly less efficient in insect cells than the analogous step in mammalian cells. Finally, the insect-derived gp55-116 was highly immunogenic in experimental animals and readily recognized by antibodies contained within HCMV-immune human serum, suggesting that this recombinant protein warrants further study as a potential HCMV subunit vaccine candidate.

Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...

Fingerprint Dive into the research topics of The critical glycosylation site of human transferrin receptor contains a high-mannose oligosaccharide. Together they form a unique fingerprint. ...

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG