Search Results | Journal of Cell Biology | Rockefeller University Press
Antigen processing and presentation and cytotoxic targeting depend on the activities of several lysosomal enzymes that require mannose 6-phosphate (M6P) sorting signals for efficient intracellular transport and localization. In this paper, we show that mice deficient in the formation of M6P residues exhibit significant loss of cathepsin proteases in B cells, leading to lysosomal dysfunction with accumulation of storage material, impaired antigen processing and presentation, and subsequent defects in B cell maturation and antibody production. The targeting of lysosomal and granular enzymes lacking M6P residues is less affected in dendritic cells and T cells and sufficient for maintenance of degradative and lytic functions. M6P deficiency also impairs serum immunoglobulin levels and antibody responses to vaccination in patients. Our data demonstrate the critical role of M6P-dependent transport routes for B cell functions in vivo and humoral immunity in mice and human ...
The cytoplasmic tail of the mannose 6-phosphate/insulin-like growth factor-II receptor has two signals for lysosomal enzyme...
The mannose 6-phosphate/insulin-like growth factor-II (Man-6-P/IGF-II) receptor is known to cycle between the Golgi, endosomes, and the plasma membrane. In the Golgi the receptor binds newly synthesized lysosomal enzymes and transports them directly to an endosomal (prelysosomal) compartment without traversing the plasma membrane. Deletion of the carboxyl-terminal Leu-Leu-His-Val residues of the 163 amino acid cytoplasmic tail of the bovine Man-6-P/IGF-II receptor partially impaired this function, resulting in the diversion of a portion of the receptor-ligand complexes to the cell surface, where they were endocytosed. The same phenotype was observed when 134 residues of the cytoplasmic tail were deleted from the carboxyl terminus. Disruption of the Tyr24-Lys-Tyr-Ser-Lys-Val29 plasma membrane internalization signal alone had little effect on Golgi sorting, but when combined with either deletion resulted in a complete loss of this function. The mutant receptors retained the ability to recycle to ...
Mannose 6-phosphate/insulin-like growth factor II-binding proteins in human serum and urine. Their relation to the mannose 6...
Human serum and urine contain polypeptides which bind mannose 6-phosphate (M6P) and insulin-like growth factor II (IGF II) and crossreact with antibodies against the M6P/IGF II receptor. These polypeptides are considered to be fragments of the M6P/IGF II receptor. The major Mr approx. 205,000 fragment in serum and urine is about 10 kDa smaller in size than the membrane-associated receptor and is accompanied by minor forms with Mr values ranging from 104,000 to 180,000. The presence of receptor fragments in biological fluids indicates that shedding is one of the mechanisms contributing to the turnover of the M6P/IGF II receptor and that receptor fragments are part of the heterogenous group of serum proteins whic bind IGF II. ...
Receptor insulinu sličnog faktora rasta 2 - Wikipedia
Schmidt B, Kiecke-Siemsen C, Waheed A et al. (1995). Localization of the insulin-like growth factor II binding site to amino acids 1508-1566 in repeat 11 of the mannose 6-phosphate/insulin-like growth factor II receptor. J. Biol. Chem. 270 (25): 14975-82. PMID 7797478. doi:10.1074/jbc.270.25.14975. CS1 održavanje: Eksplicitna upotreba et al. (link) ...
Medical Advisor Journals, Kyle J. Norton Site, Health Tips for Better Living and Living Health: Food therapy - Celery and lung...
The study of dietary intake of vegetables and fruit and lung cancer risk in Harbin, Heilongjiang province, northeast China(b) and Tin Corporation (YTC) miners in Yunnan(c), showed a positive effect of intake of celery daily in reduced risk of lung cancer. According to Dr. Belanger JT. research, Perillyl alcohol, a monoterpene isolated from the essential oils of celery seeds, exhibited the effect of inducing apoptosis in tumor cells without affecting normal cells and reverting tumor cells back to a differentiated state in very cancer cells, including lung cancer, through increased mannose-6-phosphate/insulin-like growth factor II receptors, tissue growth factor beta receptors, Bak and decreased ras protein prenylation, ubiquinone synthesis, and induced Phase I and Phase II detoxification systems(d ...
CIM6PR Definition: Cation-Independent Mannose 6-Phosphate Receptor
definition of CIM6PR, what does CIM6PR mean?, meaning of CIM6PR, Cation-Independent Mannose 6-Phosphate Receptor, CIM6PR stands for Cation-Independent Mannose 6-Phosphate Receptor
The intramitochondrial location of the glutaminase isoenzymes of pig kidney | Biochemical Journal
1. The glutaminase activity of pig kidney is located almost entirely in the cortex. 2. Pig renal cortex contains two glutaminases, one phosphate-dependent and one phosphate-independent. Both isoenzymes are localized exclusively in the mitochondria. 3. After sonication of the mitochondria, the phosphate-dependent isoenzyme is entirely soluble, whereas approximately half the phosphate-independent isoenzyme is associated with the membranes. 4. In intact mitochondria, the activities of both isoenzymes respond to changes in the pH of the intramitochondrial compartment. 5. It is concluded that both glutaminase isoenzymes are situated in the intramitochondrial compartment, and that the phosphate-independent glutaminase may be bound to the inside of the inner mitochondrial membrane.. ...
Cation-dependent mannose-6-phosphate receptor - Wikipedia
In the fields of biochemistry and cell biology, the cation-dependent mannose-6-phosphate receptor (CD-MPR) also known as the 46 kDa mannose 6-phosphate receptor is a protein that in humans is encoded by the M6PR gene. The CD-MPR is one of two transmembrane proteins that bind mannose-6-phosphate (M6P) tags on acid hydrolase precursors in the Golgi apparatus that are destined for transport to the lysosome. Homologues of CD-MPR are found in all eukaryotes. The CD-MPR is a type I transmembrane protein (that is, it has a single transmembrane domain with its C-termini on the cytoplasmic side of lipid membranes) with a relatively short cytoplasmic tail. The extracytoplasmic/lumenal M6P binding-domain consists of 157 amino acid residues. The CD-MPR is approximately 46 kDa in size and it both exists and functions as a dimer. The cell surface receptor for insulin-like growth factor 2 also functions as a cation-independent mannose 6-phosphate receptor. It consists of fifteen repeats homologous to the ...
Maternal transmission of an Igf2r domain 11: IGF2 binding mutant allele ( Igf2r I1565A ) results in partial lethality,...
The cation-independent mannose 6-phosphate/insulin-like growth factor-2 receptor (M6P/IGF2R or IGF2R) traffics IGF2 and M6P ligands between pre-lysosomal and extra-cellular compartments. Specific IGF2 and M6P high-affinity binding occurs via domain-11 and domains-3-5-9, respectively. Mammalian maternal Igf2r allele expression exceeds the paternal allele due to imprinting (silencing). Igf2r null-allele maternal transmission results in placenta and heart over-growth and perinatal lethality (|90%) due to raised extra-cellular IGF2 secondary to impaired ligand clearance. It remains unknown if the phenotype is due to either ligand alone, or to both ligands. Here, we evaluate Igf2r specific loss-of-function of the domain-11 IGF2 binding site by replacing isoleucine with alanine in the CD loop (exon 34, I1565A), a mutation also detected in cancers. Igf2rI1565A/+p maternal transmission (heterozygote), resulted in placental and embryonic over-growth with reduced neonatal lethality (|60%), and long-term survival.
Intermonomer Interactions Are Essential for Lysosomal Enzyme Binding by the Cation-Dependent Mannose 6-Phosphate Receptor
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RCSB PDB - 1C39: STRUCTURE OF CATION-DEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR BOUND TO PENTAMANNOSYL PHOSPHATE...
1C39: Structural basis for recognition of phosphorylated high mannose oligosaccharides by the cation-dependent mannose 6-phosphate receptor.
Mannose-6-phosphate receptors: Definition with Mannose-6-phosphate receptors Pictures and Photos
Definition of Mannose-6-phosphate receptors with photos and pictures, translations, sample usage, and additional links for more information.
ALZFORUM | NETWORKING FOR A CURE
Lin SX, Mallet WG, Huang AY, Maxfield FR. Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes ...
OriGene - M6PR (NM 002355) Human Protein
Recombinant protein of human mannose-6-phosphate receptor (cation dependent) (M6PR), 20 ug available for purchase from OriGene - Your Gene Company.
RCSB PDB for 4AQ0
4AQ0: A Bacterial Glycosidase Enables Mannose-6-Phosphate Modification and Improved Cellular Uptake of Yeast-Produced Recombinant Human Lysosomal Enzymes.
The insulin-like growth factor II receptor gene is mutated in genetically unstable cancers of the endometrium, stomach, and...
TY - JOUR. T1 - The insulin-like growth factor II receptor gene is mutated in genetically unstable cancers of the endometrium, stomach, and colorectum. AU - Ouyang, Hong. AU - Shiwaku, Hiromi O.. AU - Hagiwara, Hisashi. AU - Miura, Ko. AU - Abe, Tadayoshi. AU - Kato, Yo. AU - Ohtani, Haruo. AU - Shiiba, Kenichi. AU - Souza, Rhonda F.. AU - Meltzer, Stephen J.. AU - Horii, Akira. PY - 1997/5/15. Y1 - 1997/5/15. N2 - Disruption of the DNA mismatch repair system, characterized by microsatellite instability (MI), plays an important role in the course of human carcinogenesis. Repetitive sequences constitute targets for mutation in MI+ cells, and frequent mutations have indeed been reported in such regions within the transforming growth factor β receptor II (RII) gene in genetically unstable colorectal and gastric cancers. However, other genes that are targets for mutations in MI+ cells during the course of carcinogenesis have proven elusive. Because the insulin-like growth factor II receptor ...
Insulin Receptor Isoform A, a Newly Recognized, High-Affinity Insulin-Like Growth Factor II Receptor in Fetal and Cancer Cells ...
The present study demonstrates that IR-A is a physiological receptor for IGF-II. Previously it was believed that most, if not all, biological effects of IGF-II in cells were mediated by IGF-I-R. IGF-II-R, which also binds mannose-6-phosphate residues, is devoid of tyrosine kinase activity and is not believed to have either metabolic or mitogenic signaling potential. Most studies have indicated that the IR, which is homologous to the IGF-I-R, binds IGF-II with a relatively low affinity (1 to 5% that of insulin) (45). However, there is evidence that in certain instances the IR can bind IGF-II with high affinity. Atypical IRs, which bind IGF-II with unusually high affinity, have been found in IM-9 lymphoblasts, immature erythrocytes (18), and fetal tissues (including human placenta and brain, and chicken embryo fibroblasts) (19). Furthermore, other studies suggest that during mouse fetal development, the growth promoting effect of IGF-II is mediated in part by signaling through the IR (28). By ...
Insulin Receptor Isoform A, a Newly Recognized, High-Affinity Insulin-Like Growth Factor II Receptor in Fetal and Cancer Cells ...
The present study demonstrates that IR-A is a physiological receptor for IGF-II. Previously it was believed that most, if not all, biological effects of IGF-II in cells were mediated by IGF-I-R. IGF-II-R, which also binds mannose-6-phosphate residues, is devoid of tyrosine kinase activity and is not believed to have either metabolic or mitogenic signaling potential. Most studies have indicated that the IR, which is homologous to the IGF-I-R, binds IGF-II with a relatively low affinity (1 to 5% that of insulin) (45). However, there is evidence that in certain instances the IR can bind IGF-II with high affinity. Atypical IRs, which bind IGF-II with unusually high affinity, have been found in IM-9 lymphoblasts, immature erythrocytes (18), and fetal tissues (including human placenta and brain, and chicken embryo fibroblasts) (19). Furthermore, other studies suggest that during mouse fetal development, the growth promoting effect of IGF-II is mediated in part by signaling through the IR (28). By ...
Roles of heme insertion and the mannose-6-phosphate receptor in processing of the human myeloid lysosomal enzyme,...
Biosynthesis of myeloperoxidase (MPO), a myeloid lysosomal hemoprotein critical for the optimal oxygen-dependent microbicidal activity of human neutrophils, is incompletely understood. The primary translation product undergoes cotranslational N-linked glycosylation with subsequent insertion of the Fe-containing prosthetic group into the peptide backbone, thereby converting the enzymatically inactive, heme- free apoproMPO into the peroxidatively active precursor, proMPO. Eventually, proMPO undergoes proteolytic processing into native, lysosomal MPO, with subunits of 59 and 13.5 Kd. We studied three unanswered questions regarding MPO biosynthesis: (1) At what point during MPO biosynthesis is the heme moiety inserted into the apoenzyme? (2) What consequences does heme-insertion have on subsequent processing events? (3) What role does the mannose-6-phosphate receptor (M6PR) system play in the delivery of MPO to the lysosome? Disruption of Golgi by brefeldin A (BFA) produced two major changes in MPO ...
Mannose-6-phosphate receptors definition | Drugs.com
Definition of mannose-6-phosphate receptors. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
ALZFORUM | NETWORKING FOR A CURE
Arighi CN, Hartnell LM, Aguilar RC, Haft CR, Bonifacino JS. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor ...
GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Sorting of mannose 6-phosphate receptors mediated by the GGAs.
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Sorting of mannose 6-phosphate receptors mediated by the GGAs.
Mannose-6-phosphate receptor (IPR028927) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Publications | SBRC
Tiels, P., E. Baranova, K. Piens, C. De Visscher, G. Pynaert, W. Nerinckx, J. Stout, F. Fudalej, P. Hulpiau, S. Tännler, et al., A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes., Nat Biotechnol, vol. 30, issue 12, pp. 1225-31, 2012 Dec. ...
Publications | SBRC
Tiels, P., E. Baranova, K. Piens, C. De Visscher, G. Pynaert, W. Nerinckx, J. Stout, F. Fudalej, P. Hulpiau, S. Tännler, et al., A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes., Nat Biotechnol, vol. 30, issue 12, pp. 1225-31, 2012 Dec. ...
Role for dynamin in late endosome dynamics and trafficking of the cation-independent mannose 6-phosphate receptor. | DIAL.pr ...
It is well established that dynamin is involved in clathrin-dependent endocytosis, but relatively little is known about possible intracellular functions of this GTPase. Using confocal imaging, we found that endogenous dynamin was associated with the plasma membrane, the trans-Golgi network, and a perinuclear cluster of cation-independent mannose 6-phosphate receptor (CI-MPR)-containing structures. By electron microscopy (EM), it was shown that these structures were late endosomes and that the endogenous dynamin was preferentially localized to tubulo-vesicular appendices on these late endosomes. Upon induction of the dominant-negative dynK44A mutant, confocal microscopy demonstrated a redistribution of the CI-MPR in mutant-expressing cells. Quantitative EM analysis of the ratio of CI-MPR to lysosome-associated membrane protein-1 in endosome profiles revealed a higher colocalization of the two markers in dynK44A-expressing cells than in control cells. Western blot analysis showed that ...
Gene Expression Literature Detail
J:45195 Melnick M, Chen H, Buckley S, Warburton D, Jaskoll T, Insulin-like growth factor II receptor, transforming growth factor-beta, and Cdk4 expression and the developmental epigenetics of mouse palate morphogenesis and dysmorphogenesis. Dev Dyn. 1998 Jan;211(1):11-25 ...
Measurement of insulin-like growth factor-II in physiological fluids and tissues. II. extraction and quantification in rat...
TY - JOUR. T1 - Measurement of insulin-like growth factor-II in physiological fluids and tissues. II. extraction and quantification in rat tissues. AU - Lee, Wei Hua. AU - Bowsher, Ronald R.. AU - Apathy, John M.. AU - Smith, Michele C.. AU - Henry, David P.. PY - 1991/2. Y1 - 1991/2. N2 - The tissue distribution and developmental patterns of insulin-like growth factor-II (IGF-II) have not been investigated in rat tissues, primarily because of the lack of an efficient extraction method for IGF-II and a sensitive RIA. IGF- II was extracted from rat tissues by formic acid, and the extract was heated at an acidic pH and treated with acetone. The removal of binding proteins was demonstrated by fast protein liquid chromatography size exclusion column and the elimination of a dilutional bias in the RIA. Using rat IGF-II as standard, we optimized a RIA for the quantification of IGF-II in rat tissues. In adult rats, IGF-II was found in all 15 tissues examined, with the highest concentration in the ...
Real time kinetics of insulin-like growth factor II (IGF-II) interaction with the IGF-II/mannose 6-phosphate receptor: the...
The interaction of soluble forms of the human cation-independent insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-IIR) with IGFs and mannosylated ligands was analyzed in real time. IGF-IIR proteins containing domains 1-15, 10-13, 11-13, or 11-12 were combined with rat CD4 domains 3 and 4. Following transient expression in 293T cells, secreted protein was immobilized onto biosensor chips. beta-Glucuronidase and latent transforming growth factor-beta1 bound only to domains 1-15. IGF-II bound to all constructs except a control, which contained a point mutation in domain 11. The affinity of domains 1-15, 10-13, 11-13, and 11-12 to IGF-II were 14, 120, 100, and 450 nm, respectively. Our data suggest that domain 13 acts as an enhancer of IGF-II affinity by slowing the rate of dissociation, but additional enhancement by domains other than 10-13 also occurs. As the receptor functions to transport ligands from either the trans-Golgi network or extracellular space to the endosomes, the interaction
VackvSuG: Stabalized development of acinus and SRRM1
In addition to finding most of the known EJC factors splicing containing variable 5 exon (v5 meoitic and mitotic paragene T codon B cell) and correlates scaffold hnRPN-I/U, with SRM160 is the more important component of the complex; it has multiple R, S, and P residues. The acinus L, S, and S-prime cDNAs contain open reading frames (ORF) read anticlockwse-L, and clockwise-R to an abundance of unspecific YWHAG from HPRD-[§§], overexpressed amino acid residues near the exon-exon junctions of mRNAs. While a stable association of development and differentiation and ACN1 condensation such as polymerase eta ribonucleoprotein U scaffold attachment after irradiation with UVC light but another protein inhibitor is replication-independent damage accumulation of residues is not necessary for UV-induced polymerase eta focus formation. Where as RNA binding protein S1, intronless [wild type] versions of SRm160 is normally also dependent on U1-2 (igG) certain non-T/non-B-ALL, cells. Small nuclear RNPs an ...
Produk Man- Dye - GLOBElife ☎ - Keune - Produk untuk rambut, Produk Hair: Color Man, Perawatan Saluran Man : Pengobatan Dan...
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Characteristics of the internalization signal in the Y543 influenza virus hemagglutinin suggest a model for recognition of...
TY - JOUR. T1 - Characteristics of the internalization signal in the Y543 influenza virus hemagglutinin suggest a model for recognition of internalization signals containing tyrosine. AU - Naim, Hussein Y.. AU - Roth, Michael G.. N1 - Copyright: Copyright 2005 Elsevier B.V., All rights reserved.. PY - 1994/2/11. Y1 - 1994/2/11. N2 - Several proteins, including the hemagglutinin (HA)-Y543 mutant influenza virus hemagglutinin, are internalized by clathrin-coated pits but do not have a sequence that fits a recently proposed consensus for internalization signals containing tyrosine. To determine whether or not the HA-543 signal is a degenerate form of the internalization signal found in proteins such as the transferrin receptor and mannose 6-phosphate/insulin-like growth factor (IGF) II receptor, we have mutated amino acid positions of HA-Y543 shown to be important for internalization of the two receptors. Our results indicate that the HA-Y543 mutant contains a suboptimum sequence for a ...
Insulin-like growth factor-II/Mannose-6-phosphate receptor in the spinal cord and dorsal root ganglia of the adult rat - Open...
The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor is a multifunctional transmembrane glycoprotein, which interacts with a number of molecules, including IGF-II and M6P-containing lysosomal enzymes. The receptor is widely distributed throughout the brain and is known to be involved in lysosomal enzyme trafficking, cell growth, internalization and degradation of IGF-II. In the present study, using autoradiographic, Western blotting and immunocytochemical methods, we provide the first report that IGF-II/M6P receptors are discretely distributed at all major segmental levels of the spinal cord and dorsal root ganglia of the adult rat. In the spinal cord, a high density of [125I]IGF-II binding sites was evident in the ventral horn (lamina IX) and in areas around the central canal (lamina X), whereas intermediate grey matter and dorsal horn were associated with moderate receptor levels. The dorsal root ganglia exhibited rather high density of [125I]IGF-II binding sites. ...
EMBL: CP001994.PE53
CP001994.PE53 Location/Qualifiers FT CDS_pept 59450..60745 FT /codon_start=1 FT /transl_table=11 FT /locus_tag=Mmah_0054 FT /product=Phosphoglucosamine mutase FT /EC_number=5.4.2.10 FT /note=COGs: COG1109 Phosphomannomutase; FT InterProIPR005841:IPR005844:IPR005846:IPR005843:IPR FT 005845:IPR016066; KEGG: cla:Cla_1567 FT phosphoglucomutase/phosphomannomutase family protein; PFAM: FT phosphoglucomutase/phosphomannomutase alpha/beta/alpha FT domain I; phosphoglucomutase/phosphomannomutase FT alpha/beta/alpha domain III; FT phosphoglucomutase/phosphomannomutase; FT phosphoglucomutase/phosphomannomutase alpha/beta/alpha FT domain II; PRIAM: Phosphoglucosamine mutase; SPTR: Q46AY7 FT Probable phosphoglucosamine mutase; PFAM: FT Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha FT domain II; Phosphoglucomutase/phosphomannomutase, FT alpha/beta/alpha domain III; FT Phosphoglucomutase/phosphomannomutase, C-terminal domain; FT Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha FT domain ...
Phosphomannomutase elisa and antibody
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Insulin-Like Growth Factor-II in Human Tumour Cell Proliferation - Cancer Council Victoria
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Cell Biology Lab - Vrije Universiteit Brussel
42. De Bleser, P., P. Hannes, S. Van Buul-Offers, C. Hoogerbrugge, C. Van Schravendijk, T. Niki, V. Rogiers, J. Van den Brande, E. Wisse, and A. Geerts. 1995. Increased insulin-like growth factor-II/mannose 6-phosphate receptor on fibrotic rat fat-storing cells facilitates activation of latent transforming growth factor- . In Cells of the Hepatic sinusoid. E. Wisse, D.L. Knook, and K. Wake, editors. The Kupffer Cell Foundation, Leiden. 383-385 ...
Inter cellular exchange of lysosomal enzymes enzyme assays in single human fibroblasts after co cultivation - GeoScience.net
Reuser, A.; Halley, D.; De-Wit, E.; Hoogeveen, A.; Van-Der-Kamp, M.; Mulder, M.; Galjaard, H., 1976: Inter cellular exchange of lysosomal enzymes enzyme assays in single human fibroblasts after co cultivation
D-Mannose With Cranactin - Solaray | Sanavita
Mannose With Cranactin lupta natural impotriva infectiillor urinare. Cumpara acum Mannose With Cranactin la un pret special cu livrare rapida din stocul Sanavita
Loss of Imprinting of Insulin-like Growth Factor-II in Wilms Tumor Commonly Involves Altered Methylation but not Mutations of...
Loss of Imprinting of Insulin-like Growth Factor-II in Wilms Tumor Commonly Involves Altered Methylation but not Mutations of CTCF or Its Binding ...
In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor. - NDM Research Building
Hereditary hemochromatosis is most frequently associated with mutations in HFE, which encodes a class Ib histocompatibility protein. HFE binds to the transferrin receptor-1 (TfR1) in competition with iron-loaded transferrin (Fe-Tf). HFE is released from TfR1 by increasing concentrations of Fe-Tf, and free HFE may then regulate iron homeostasis by binding other ligands. To search for new HFE ligands we expressed recombinant forms of HFE in the human cell line 293T. HFE protein was purified, biotinylated and made into fluorescently labelled tetramers. HFE tetramers bound to TfR1 in competition with Tf, but in addition we detected a binding activity on some cell types that was not blocked by Fe-Tf or by mutations in HFE that prevent binding to TfR1. We identified this second HFE ligand as the cation independent mannose-6-phosphate receptor (CI-MPR, also known as the insulin-like growth factor-2 receptor, IGF2R). HFE:CI-MPR binding was mediated through phosphorylated mannose residues on HFE. Recombinant
pcampeau
Mucolipidosis II (I-cell disease) and mucolipidosis IIIA (classical pseudo-hurler polydystrophy) are caused by mutations in the GlcNAc-phosphotransferase alpha / beta -subunits precursor gene. Kudo M, Brem MS, Canfield WM Am J Hum Genet. 2006 Mar;78(3):451-63. For more information on Mucolipidosis II and mucolipidosis IIIA, please visit OMMBID Chapter 138. Thank you very much in advance […]. ...
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phospholipid, Biological macromolecules: lipids, By OpenStax | Jobilize
major constituent of the membranes; composed of two fatty acids and a phosphate-containing group attached to a glycerol backbone,
ALPHA-DELTAUA-2S-[1-|4]-GLCNAC-6S
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Insulin-like growth factor-II in nonislet cell tumors associated with hypoglycemia: Increased levels of messenger ribonucleic...
TY - JOUR. T1 - Insulin-like growth factor-II in nonislet cell tumors associated with hypoglycemia. T2 - Increased levels of messenger ribonucleic acid. AU - Lowe, William L.. AU - Roberts, Charles T.. AU - Leroith, Derek. AU - Rojeski, Maria T.. AU - Merimee, Thomas J.. AU - Fui, Serge Teng. AU - Keen, Harry. AU - Arnold, Dagmar. AU - Mersey, James. AU - Gluzman, Sheldon. AU - Spratt, Daniel. AU - Eastman, Richard C.. AU - Roth, Jesse. PY - 1989/12. Y1 - 1989/12. N2 - The role of insulin-like growth factor-II (IGFII) in the hypoglycemia associated with nonislet cell tumors is controversial. In this study we have addressed this question by measuring the IGF-II mRNA levels in extracts of these tumors. Hybridization of a 32P-labeled IGF-II cDNA to a Northern blot of RNA from three nonislet cell tumors associated with hypoglycemia (a hemangiopericytoma, fibrosarcoma, and malignant mesenchymal tumor) demonstrated six hybridizing bands, 6.8, 5.6, 4.7, 3.6, 2.6, and 2.1 kilobases in length. These ...
Lysosomal dysfunction causes neurodegeneration in mucolipidosis II knock-in mice<...
TY - JOUR. T1 - Lysosomal dysfunction causes neurodegeneration in mucolipidosis II knock-in mice. AU - Kollmann, K.. AU - Damme, M.. AU - Markmann, S.. AU - Morelle, W.. AU - Schweizer, M.. AU - Hermans-Borgmeyer, I.. AU - Röchert, A. K.. AU - Pohl, S.. AU - Lübke, T.. AU - Michalski, J. C.. AU - Käkelä, R.. AU - Walkley, S. U.. AU - Braulke, T.. PY - 2012/9. Y1 - 2012/9. N2 - Mucolipidosis II is a neurometabolic lysosomal trafficking disorder of infancy caused by loss of mannose 6-phosphate targeting signals on lysosomal proteins, leading to lysosomal dysfunction and accumulation of non-degraded material. However, the identity of storage material and mechanisms of neurodegeneration in mucolipidosis II are unknown. We have generated knock-in mice with a common mucolipidosis II patient mutation that show growth retardation, progressive brain atrophy, skeletal abnormalities, elevated lysosomal enzyme activities in serum, lysosomal storage in fibroblasts and brain and premature death, ...
Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7 | JCB
The retromer is a phylogenetically conserved multisubunit complex that mediates retrograde transport of transmembrane cargo from endosomes to the TGN (Seaman, 2005; Bonifacino and Rojas, 2006; Bonifacino and Hurley, 2008). The best-characterized cargo for the mammalian retromer is the cation-independent mannose 6-phosphate receptor (MPR [CI-MPR]), one of two intracellular sorting receptors that participates in the delivery of acid hydrolases to lysosomes (Kornfeld, 1992). The CI-MPR binds newly synthesized acid hydrolases at the TGN and carries them within clathrin-coated vesicles to endosomes, where the hydrolases are released for eventual transport to lysosomes. The retromer functions to retrieve the unoccupied receptors to the TGN, where they engage in further cycles of acid hydrolase sorting. Depletion of retromer subunits by RNAi prevents this retrieval, leading to rerouting of the receptors to lysosomes and consequent leakage of newly synthesized acid hydrolases into the extracellular ...
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The obligate human pathogen Neisseria gonorrhoeae is responsible for the widespread sexually transmitted disease gonorrhoea, which in rare cases also leads to the development of disseminated gonococcal infection (DGI). DGI is mediated by PorBIA-expressing bacteria that invade host cells under low phosphate condition by interaction with the scavenger receptor-1 (SREC-I) expressed on the surface of endothelial cells. The interaction of PorBIA and SREC-I was analysed using different in vitro approaches, including surface plasmon resonance experiments that revealed a direct phosphate-independent high affinity interaction of SREC-I to PorBIA. However, the same binding affinity was also found for the other allele PorBIB, which indicates unspecific binding and suggests that the applied methods were unsuitable for this interaction analysis. Since N. gonorrhoeae was recently classified as a super-bug due to a rising number of antibiotic-resistant strains, this study aimed to discover inhibitors against ...
The mannose 6-phosphate receptor and the biogenesis of lysosomes<...
TY - JOUR. T1 - The mannose 6-phosphate receptor and the biogenesis of lysosomes. AU - Griffiths, Gareth. AU - Hoflack, Bernard. AU - Simons, Kai. AU - Mellman, Ira. AU - Kornfeld, Stuart. PY - 1988/2/12. Y1 - 1988/2/12. N2 - Localization of the 215 kd mannose 6-phosphate receptor(MPR) was studied in normal rat kidney cells. Low levels of receptor were detected in the trans Golgi network, Golgi stack, plasma membrane, and peripheral endosomes. The bulk of the receptor was localized to an acidic, reticular-vesicular structure adjacent to the Golgi complex. The structure also labeled with antibodies to lysosomal enzymes and a lysosomal membrane glycoprotein (Igp120). While lysosome-like, this structure is not a typical lysosome that is devoid of MPRs. The endocytic marker α2macroglobulin-gold entered the structure at 37°C, but not at 20°C. With prolonged chase, most of the marker was transported from the structure into lysosomes. We propose that the MPR/Igp-enriched structure is a specialized ...
β1 Integrins Modulate Cell Adhesion by Regulating Insulin-Like Growth Factor-II Levels in the Microenvironment | Cancer Research
Reagents and antibodies. Mouse Laminin-1, Lipofectin, LipofectAMINE, and LipofectAMINE 2000 were purchased from Invitrogen (Carlsbad, CA). Recombinant human IGF-I or IGF-II (rhIGF-II) was purchased from R&D System, Inc. (Minneapolis, MN) or Austral Biologics (San Ramon, CA), respectively. Human FN was purified as described ( 33). Bovine serum albumin (BSA) was purchased from Sigma (St. Louis, MO). Wortmannin was purchased from Calbiochem (La Jolla, CA).. The following monoclonal antibodies (mAbs) were used: to human β1 integrin P4C10 (Chemicon, Temecula, CA), clone-18 (BD Biosciences, San Jose, CA), and TS2/16 [American Type Culture Collection (ATCC), Manassas, VA]; to chicken β1 integrin W1B10 (Sigma Chemical Co., St. Louis, MO); to human β4 integrin A9 (kindly provided by Dr. L. Shaw); to hemagglutinin 12CA5 (ATCC); to a vascular endothelial surface protein 1C10 (Life Technologies, Inc., Gaithersburg, MD); to c-myc; to β-tubulin (Sigma). The following rabbit polyclonal antibodies were ...
GTOP nfar0:BAD55805.1
GT:ID BAD55805.1 GT:GENE BAD55805.1 GT:PRODUCT putative phosphomannomutase GT:DATABASE GIB00210CH01 GT:ORG nfar0 GB:ACCESSION GIB00210CH01 GB:LOCATION complement(1059582..1061099) GB:FROM 1059582 GB:TO 1061099 GB:DIRECTION - GB:PRODUCT putative phosphomannomutase GB:PROTEIN_ID BAD55805.1 LENGTH 505 SQ:AASEQ MERTRSLAMLRFGTAGLRGPVGPGPDAMNTETVARATAGVVEWLRGRCLGGGRVVVGRDARHGSAEFAAGTAEIFQAAGFPVTLLPEPLPTPVVAYAVRALGAVAGVQITASHNPPADNGYKLYLDGGSQLIAPADAEIERCIEAARPQFDRAAVAPSGAELVDDYLARVAALPSLIGGPGPRAAVRIALTPMHGVGGDLAVRALAAAGFTDVHVVPEQFDPDPDFPTVAFPNPEEPGAADLVLATARRVGADLAIALDPDADRCALGVPGPDGWRMLRGDETGVLLGDRVLRTAPADALVATTIVSSRLLSKVATARGARYAETLTGFKWLARAGDGLVYAYEEAIGHCVDPATVRDKDGISAAVTAADMVAAGKAAGRTLLDELDDYAVRFGVHAGDQVSLRTADTAAAAAVVERLRADPPAALAGEPMRYTDQLRVRGRLRTDALIFEGERSRVVIRPSGTEPKLKCYLEVVEPVRAPAALAAAKQAARERLAELADYCRRL GT:EXON 1,1-505:0, BL:SWS:NREP 1 BL:SWS:REP 28-,471,Y740_HAEIN,1e-62,36.0,433/485, PROS 106-,115,PS00710,PGM_PMM,PDOC00589, SEG 45-,63,rgrclgggrvvvgrdarhg, SEG 81-,94,pvtllpeplptpvv, SEG ...
BILIARY EXCRETION OF LYSOSOMAL PROTEIN - Mayo Clinic
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint ...
OriGene - S1PR1 (NM 001400) Human ORF cDNA Clone
S1PR1 - S1PR1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate receptor 1 (S1PR1) available for purchase from OriGene - Your Gene Company.
DEGREASERS
The TSPE is a phosphate free degreaser (100% BIODEGRADABLE) that performs as well as regular TSP, but without the harmfull side effects. The Technical avantages of using TSPE over regular TSP are : Save time (NO RINSING REQUIRED), leaves a neutral ph on painted surface, doesnt damage the surface (no phosphate residue), degreases unpainted wood without changing its color, no need to paint the surface immediately. ...
Man, 46, Rapes 16-yr-old Housemaid | Anythingnaija
Men of Ogun State Police Command have arrested 42-year-old Abiodun Amosu for defiling his-16-year-old housemaid man-46-rapes-16-yr-old-housemaid