Numerous mechanisms have been suggested for how bacterial toxins kill susceptible mammalian cells. Several recent studies demonstrated the importance of mitochondrial targeting of toxins produced by H. pylori, C. difficile, and S. aureus to mitochondria (56). In these cases toxin-mediated cell death was caspase independent and did not result in typical PTPs in the MOM (14, 18).. Previously, we reported that M. haemolytica LKT induces apoptosis of BL-3 cells in a caspase-9-dependent manner and that in mitochondria isolated from LKT-intoxicated BL-3 cells there was gross damage to the MOM (4). Based on these observations, we hypothesized that LKT is transported into the cell and binds directly to mitochondria. In the present study, we first demonstrated that full-length LKT protein could be identified in purified mitochondrial lysates from LKT-treated BL-3 cells (Fig. 1A). Transfection of anti-LKT antibodies into BL-3 cells prevented binding of LKT to mitochondria. Confocal microscopy and flow ...
Citation: Hauglund, M.J., Tatum, F.M., Bayles, D.O., Maheswaran, S.K., Briggs, R.E. 2013. Genome sequences of Mannheimia haemolytica serotype A1 strains D153 and D193 from bovine pneumonia. Genome Announcements. 1(5). DOI: 10.1128/genomeA.00848-13. Interpretive Summary: The bacterium Mannheimia haemolytica is a prominent and costly cause of respiratory disease in domestic cattle. During periods of stress or concurrent respiratory viral infection some serotypes of the bacterium proliferate dramatically, increasing the odds of progression to pneumonic disease. The genomes of two isolates which exhibit dramatic proliferation were sequenced to facilitate molecular characterization of the phenomenon and the potential development of novel disease control methods. Technical Abstract: Here we report two genomes, one complete and one draft, from virulent bovine strains of Mannheimia haemolytica serotype A1 recovered prior to the field usage of modern antimicrobial drugs. ...
The optimal production of P. haemolytica leukotoxin in the culture supernatant of a fluid medium is dependent on a number of factors. The leukotoxin has to be produced by using a strain that is known for its ability to produce high quantities of leukotoxin, inoculated into the most suitable type of medium at the correct culture density containing the necessary supplements and harvested after a certain growth period. The volume in which it is produced may also have an influence. Two different procedures are described to produce the leukotoxin in 5 to 15-ℓ quantities in RPMI 1640 medium. The first method used to produce leukotoxin is one that has been repeatedly described since the presence of the leukotoxin was first established in 1978. Using this method seven batches of leukotoxin were produced in litre quantities with leukotoxin activity ranging from 23-67 u/mℓ. The seed culture inoculum is prepared in brain heart infusion broth, which is centrifuged before the organisms are inoculated ...
This study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty Holstein calves with dairy calf pneumonia from dairy farms of Mashhad Suburb between September 2002 and August 2003 . The most common micro-organisms isolated were Pasteurella multocida 80 (61.54%), Mannheimia haemolytica 41 (31.54%), Bacillus sp. 15 (11.54%), Staphylococcus sp. 3 (2.31%), Streptococcus sp. 4 (3.08%), Pseudomonas sp. 3 (2.31%), Proteus sp. 3 (2.31%) and E coli 5 (3.84%). Antimicrobial susceptibility testing was performed on all M. haemolytica and P. multocida employing the disk diffusion method (Kirby-Bauer). Each strain was tested with 10 antimicrobial agents. With 7 (17.08%), 6 (14.63%), 4 (9.75%) and 1 (2.44%) of M. haemolytica were resistant to lincomycin, gentamicin, oxytetracycline and chloramphenicol, respectively. However, resistance to penicillin, lincomycin, amoxicillin, gentamicin and oxytetracycline was observed in 10 (12.50%), 6 (7.50%), 6 (7.50%
In the present study, recombinant proteins representing the transmembrane domain of M. haemolytica outer membrane protein A (OmpA) from a bovine serotype A1 isolate (rOmpA1) and an ovine serotype A2 isolate (rOmpA2) were over-expressed, purified and used to generate anti-rOmpA1 and anti-rOmpA2 antibodies, respectively. An examination of the binding specificities of these antibodies to M. haemolytica isolates representing different OmpA subclasses revealed that cross-absorbed anti-rOmpA1 antibodies recognised OmpA1-type proteins but not OmpA2-type proteins; conversely, cross-absorbed anti-rOmpA2 antibodies recognised OmpA2-type proteins but not OmpA1 type proteins. This demonstrated that OmpA1 and OmpA2 are surface-exposed and could potentially bind to different receptors in cattle and sheep. The outer membrane subproteomes of seven M. haemolytica isolates and one M. glucosida isolate were also characterised and compared after growth in complex growth medium in order to identify OMPs with ...
Mannheimia haemolytica is a respiratory pathogen affecting cattle and related ruminants worldwide. M. haemolytica is commonly associated with bovine respiratory disease complex (BRDC), a polymicrobial multifactorial disease.Wepresent the first two complete closed genome sequences of this species, determined using an automated assembly pipeline requiring no manual finishing.
Calves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Antibody in serum, nasal washings, and bronchoalveolar washings was titrated sequentially before and after calves were vaccinated and then challenge exposed with live homologous bacteria. Bronchoalveolar washings were collected by fiberoptics bronchoscopy, and antibody was titrated by indirect (antiglobulin) bacterial agglutination. Responsiveness to vaccination was related in initial Show moreCalves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Antibody in serum, nasal washings, and bronchoalveolar washings was titrated sequentially before and after calves were vaccinated and then challenge exposed with live homologous bacteria. Bronchoalveolar washings were collected by fiberoptics bronchoscopy, and antibody was titrated by indirect (antiglobulin) bacterial agglutination. Responsiveness to vaccination was related in initial serum ...
HORADAGODA, N. U., DE ALWIS, M. C. L., WETTIMUNY, S. G. D. S., & VIPULASIRI, A. A. (2017). BIOCHEMICAL AND CULTURAL CHARACTERISTICS OF PASTEURELLA HAEMOLYTICA ISOLATED FROM PNEUMONIC LUNGS OF GOATS. Journal of the National Science Foundation of Sri Lanka, 22(4), 325-333. DOI: http://doi.org/10.4038/jnsfsr.v22i4.8135. ...
Foreseeable future feeding trials need to for that reason take into account the use of larger inclusion of 1474110-21-8 antioxidants in feeds making use of such
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be strongly dependent on the sample preparation. Furthermore, the interfering effect of lipopolysaccharides in the sample could be successfully suppressed by adding polymyxin B. We reached a maximum relative P95 value of 14%, which is more than ...
Recommended for vaccination of healthy cattle 60 days of age or older as an aid in the prevention of respiratory disease due to Mannheimia (Pasteurella) haemolytica and Pasteurella multocida. PULMO-GUARD PHM contains a toxoid as well as cell associated antigens from multiple isolates of M. haemolytica Type A-1. PULMO-GUARD cattle vaccines stimulate a healthy immune system to build antibodies and stimulate production of antibodies that neutralize leukotoxins produced by M. haemolytica.Dosage: 2 ml subcut in the neck, repeat in 14-28 days. Revaccinate annually. 21 day slaughter withdrawal. May cause reduced milk production in lactating dairy cattle. Anaphylactoid reactions may occur. Antidote: Epinephrine.U.S. Lic. No. 315.View Manufacturer and/or Label Information: Pulmo-Guard® PH-M
Fast Shipping. Great Low Price. Endovac-Beef with ImmunePlus is used in cattle for prevention of the effects of endotoxemia due to Escherichia coli, Salmonella typhimurium, Pasteurella multocida, and Mannheimia haemolytica. Endovac-Beef with ImmunePlus is derived mutagenically from Salmonella typhimurium bacterin Re-17. Inject 2 ml into the musculature of healthy cattle. Repeat in 2 or 3 weeks. Administer a 2 ml booster dose annually.Endovac-Beef with ImmunePlus Cattle Vaccine Immvac Salmonella
Smoother cost effective prevention of Pneumonias caused by Pasteurella Multocida and Mannheimia Haemolytica.. Can be used as an aid in Salmonella and E. Coli scours prevention.. Built in Immune-Stimulant. Improves overall herd health and response.. ENDOVAC-Beef with IMMUNE Plus® improves feed to gain ratio in finishing steers and average daily gains in finishing steers.. Protecting herd health with ENDOVAC-Beef® with IMMUNE Plus® begins with a call to your veterinarian.. Call your local rep to learn more about usage and benefits on your operation. Also, look at the testimonials on the website.. ...
Fast Shipping. Great Low Price. IBR, BVD, PI3, BRSV and Mannheimia haemolytica. Give 5 ml SQ. Repeat in 2-4 weeks and once annually. Calves vaccinated before 6 months of age should be revaccinated at 6 months of age. Safe for pregnant cows.Triangle 4 + PH-K Cattle Vaccine Boehringer Ingelheim Killed Virus | Respiratory Viral | Cattle Va
Bovilis Bovipast RSP contains inact. BRS Virus strain EV908, Pi-3 Virus strain SF-4 Reisinger and Mannheimia haemolytica A1 strain M4/1 ...
Sutherland, D.R., Abdullah, K.M., Cyopick, P. and Mellors, A. Cleavage of the cell-surface O-sialoglycoproteins CD34, CD 43, CD (1992). „44, and CD45 by a novel glycoprotease from Pasteurella haemolytica". J. Immunol. 148: 1458-1464. PMID 1371528 ...
PULMO-GUARD PHM-1 helps prepare calves for exposure to M. Haemolytica and P. multocida in two ways.  First, it stimulates the healthy immune system to build antibodies that attack bacterial antigens; second it stimulates production of antibodies that neutralize leukotoxins produced by M. haemolytica.
Restriction analysis profiles of phages from M. haemolytica isolates single-digested with TaqI (lines 1-7).Legend: φPHL-1 (from strain BAA-410); φA1; φA2;
|strong|Mouse anti Bovine CD1w3 antibody, clone CC43 |/strong|recognizes the bovine CD1w3 cell surface antigen, a ~44 kDa molecule expressed as a heterodimer with beta 2 microglobulin.|br||br|Bov…
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In a data-driven world where everything is connected and businesses operate in real time, instant access to data and analytics is critica...
The objective of my research was to generate novel information concerning the epidemiology, diagnosis and prevention of bovine respiratory disease complex (BRDC), a common pre-weaning and post-weaning beef calf disease. To reach my objective, I conducted three prospective field trials within post-weaned calf populations, and one retrospective study of pre-weaned calves utilizing survey data. I evaluated differences in behavior, health and performance in calves receiving multiple component health programs. Calves in a minimally invasive program, which included primarily non-injectable products, displayed less aversion to initial product administration but experienced higher BRDC morbidity (P = 0.02) and poorer performance (P = 0.04) compared to calves in a more invasive (all injectable products) program. Secondly, in a study of Mannheimia haemolytica inoculated calves, I found that no parameter included in physical examinations, or common blood component evaluations could discern health from ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Chronic endemic respiratory disease of dairy calves and an important component of bovine respiratory disease complex. It primarily affects calves up to six months of age and the etiology is multifactorial. Stress plus a primary viral infection is followed by a secondary bacterial infection. The latter is most commonly associated with PASTEURELLA MULTOCIDA producing a purulent BRONCHOPNEUMONIA. Sometimes present are MANNHEIMIA HAEMOLYTICA; HAEMOPHILUS SOMNUS and mycoplasma species.
The clinical efficacy of doxycycline (Doxycen(R), Cenavisa, Spain), a long-acting preparation, was evaluated for treatment of Pasteurella haemolytica infection in 6 goats. One goat was not infected and served as a control. The disease was induced by intratracheal inoculation of 107 to 109 cfu of P. haemolytica. Confirmation of respiratory disease was based on evidence of appropriate clinical signs. Before and after initiation of doxycycline treatment on day 10, each goat was examined daily. Three clinical responses to doxycycline treatment were noted. Mean rectal temperatures decreased from 40.1 oC to normal, while mean respiratory rate decreased from the pre-treatment value of 32 to 27/min after 4 days. Other clinical signs associated with pneumonia resolved within 3-5 days post treatment. In addition the minimum inhibitory concentration of DOTC for the P. haemolytica isolate was found to be ,0.5 mg/mℓ. The present study indicates that DOTC may be a useful antimicrobial agent in the treatment ...
We investigated three bovine respiratory pathobionts in healthy cattle using qPCR optimised and validated to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynamic range. A longitudinal study was conducted to investigate the carriage and density of these bacteria in the nasal passages of healthy beef calves (N = 60) housed over winter in an experimental farm setting. The three pathobiont species exhibited remarkably different carriage rates and density profiles. At housing, high carriage rates were observed for P. multocida (95%), and H. somni (75%), while fewer calves were positive for M. haemolytica (13%). Carriage rates for all three bacterial species declined over the 75-day study, but not all individuals became colonised despite sharing of environment and airspace. Colonisation patterns ranged from continuous to intermittent and were different among pathobiont species. Interval-censored exponential survival models estimated the median duration of H. somni
Synonyms for bovine respiratory disease in Free Thesaurus. Antonyms for bovine respiratory disease. 22 synonyms for bovine: cow-like, taurine, calf-like, cattle-like, dull, heavy, slow, thick, stupid, dense, sluggish, lifeless, inactive, inert, lethargic, dozy. What are synonyms for bovine respiratory disease?
One Shot Ultra 8 Cattle Vaccine aids in the prevention of blackleg, malignant edema, bacilary hemoglobinuria, black disease, enterotoxemia and enteritis, and bovine pneumonia.
Bovine respiratory disease complex (BRDC) is a multi-factorial disease in which numerous factors, such as animal management, pathogen exposure and environm..
The antimicrobial properties of tulathromycin were investigated for M.haemolytica and P.multocida. Three invitro indices of antimicrobial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill curves, were established for six isolates of each organism. Each index was measured in two growth media: Mueller-Hinton broth (MHB) and calf serum. It was shown that MICs and MBCs were markedly lower in serum than in MHB. MHB:serum ratios for MIC were 47:1 (M.haemolytica) and 53:1 (P.multocida). For both serum and MHB, adjustment of pH led to greater potency at alkaline compared to acid pH. Tulathromycin MIC was influenced by size of inoculum count, being 4.0- to 7.7-fold greater for high compared to low initial counts. It was concluded that for the purpose of determining dosages for therapeutic use, pharmacodynamic data for tulathromycin should be derived in biological fluids such as serum. It is hypothesized that invitro measurement of MIC in broth, ...
Bovine respiratory disease (BRD) is a multifactorial and complex disorder caused due to combination of more than one microbial pathogens, impaired host...
1993) Economic, clinical and functional consequences of a treatment using metrenperone during an outbreak of shipping fever in ...
The present invention provides an atherectomy catheter which has a cutting element that is able to cut both soft tissue and hard tissue, and methods of cutting material from a blood vessel lumen using a rotating cutting element. The cutting element has a sharp cutting edge that surrounds a cup-shaped surface and at least one surface of abrasive material. The cup-shaped surface directs the cut material into a tissue chamber. The cutting edge and the cup-shaped surface together are well suited to cut and remove relatively soft tissue from the blood vessel. The abrasive material surface in combination with the cutting element is well suited to abrade and remove hard material from the blood vessel.
In some countries, you might find that the Women on Web website is censored. Here you can read about several ways you… Read more » ...
In some countries, you might find that the Women on Web website is censored. Here you can read about several ways you… Read more » ...
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4F7C: Crystal Structures of Bovine CD1d Reveal Altered αGalCer Presentation and a Restricted A Pocket Unable to Bind Long-Chain Glycolipids.
The role of interleukin-8 (IL-8) as a neutrophil chemoattractant in bovine pneumonic pasteurellosis was investigated by characterizing: (1) the expression of IL-8 in the lesions of pneumonic pasteurellosis, (2) the in vitro and in vivo effects of recombinant bovine IL-8 on neutrophil chemotaxis, and (3) the importance of IL-8 as a neutrophil chemoattractant in this disease. The expression of IL-8 in bovine pneumonic pasteurellosis was assessed by Northern analysis, in situ hybridization and enzyme-linked immunosorbent assay. The expression of IL-8 mRNA was elevated dramatically in lesional lung compared to non-lesional lung, viral pneumonia, and normal lung. In situ hybridization revealed intense IL-8 mRNA expression in alveolar macrophages and neutrophils, and milder expression in several other cell types. Bronchoalveolar lavage (BAL) fluid from lesional lung contained 16.06 ± 4.00 ng/ml IL-8, but lower levels were present in non-lesional lung, viral pneumonia, and normal lung (mean ...
Epizootic pneumonia of bighorn sheep is a devastating disease of uncertain etiology. To help clarify the etiology, we used culture and culture-independent methods to compare the prevalence of the bacterial respiratory pathogens Mannheimia haemolytica, Bibersteinia trehalosi, Pasteurella multocida, and Mycoplasma ovipneumoniae in lung tissue from 44 bighorn sheep from herds affected by 8 outbreaks in the western United States. M. ovipneumoniae, the only agent detected at significantly higher prevalence in animals from outbreaks (95%) than in animals from unaffected healthy populations (0%), was the most consistently detected agent and the only agent that exhibited single strain types within each outbreak. The other respiratory pathogens were frequently but inconsistently detected, as were several obligate anaerobic bacterial species, all of which might represent secondary or opportunistic infections that could contribute to disease severity. These data provide evidence that M. ovipneumoniae plays a
R.L. Larson, D.L. Step, Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex, Veterinary Clinics of North America: Food Animal Practice, 2012, 28, 1, ...
Bovi-Sera Serum Antibodies from Colorado - For use as an aid in the prevention and treatment of enteric and respiratory conditions in cattle and sheep caused by Arcanobacterium pyogenes, E. coli, Mannheimia haemolytica, Pasteurella multocida and Salmonella typhimurium. Administer IM or subcut.
Pasteurellosis in Cattle Also known as: Transit Fever, Shipping Fever Pneumonic pasteurellosis is most commonly seen in recently weaned, single-suckled beef calves after housing or transport to a new herd/premises.
Pasteurella species isolated from the bovine respiratory tract and their antimicrobial sensitivity patterns.: Pasteurella haemolytica biotype A, serotype 1 (P h
Pasteurellosis is an infectious disease caused byPasteurella multocida, a Gram-negative coccobacillus .The disease is characterized by congestion and haemorrhage of young skin. The bacteria may enter blood leading to septicemia.
The cost of Bovine Respiratory Disease to the beef industry due to death, poorer conversions, and therapy is estimated to cost more than $3 billion per year. Identifying and mitigating Bovine Respiratory Disease in cattle can be difficult due to the increased susceptibility for Bovine Respiratory Disease in high risk cattle. One management option to minimize an outbreak of respiratory disease is the use of metaphylaxis, the mass treatment of a group of calves to reduce the incidence and adverse effects of respiratory disease on high risk animals. Criteria used to determine the necessity of metaphylactic treatment against Bovine Respiratory Disease in feedlots can be based on several factors depending on feedlot preference; however, the primary criteria often considered are: a known history of no previous vaccinations, overall appearance of cattle, source of cattle, Bovine Respiratory Disease in calves received from same source previously, long shipping distance, season of the year, and light arrival
2 PULSED-FIELD GEL ELECTROPHORESIS (PFGE) (a) Basic principles A solution to the problems of analysis caused by complex chromosomal fingerprint patterns is to use a rare-cutting restriction endonuclease to generate only a limited number of large DNA fragments. As stated in the previous section, such large fragments cannot be separated readily by conventional agarose gel electrophoresis. The only technique available currently that is capable of separating DNA molecules in the 50 Analysis of chromosomal DNA 45 kb-12 Mb range physically is pulsed-field gel electrophoresis (PFGE), first described by Schwartz and Cantor (1984). 345-76. B. I. (1989) Serotyping Bordetella pertussis strains. Vaccine, 7, 491-4. , Polveroni, G. and Abadie, G. (1988) Serotyping of Pasteurella haemolytica - comparison and adjustment of antigenic extracts and techniques. Revue de Medecine Veterinaire, 139, 719-22. , Nicklon, S. R. (1977) DNA sequencing with chainterminating inhibitors. Proceedings of the National Academy ...
Florfenicol is a synthetic, broad-spectrum antibiotic active against many Gram-negative and Gram-positive bacteria isolated from domestic animals. It acts by binding to the 50S ribosomal subunit and inhibiting bacterial protein synthesis. Florfenicol is generally considered a bacteriostatic drug, but it exhibits bactericidal activity against certain bacterial species. In vitro studies demonstrate that florfenicol is active against the BRD pathogens M. haemolytica, P. multocida, H. somni, and M. bovis and that florfenicol exhibits bactericidal activity against strains of M. haemolytica and H. somni. The minimum inhibitory concentrations (MICs) of florfenicol were determined for BRD isolates obtained from calves enrolled in BRD field studies in the U.S. in 2006 using methods recommended by the Clinical and Laboratory Standards Institute (M31-A2). Isolates were obtained from pre-treatment nasal swabs from all calves enrolled at all four sites, post-treatment nasal swabs from treatment failures in ...