TY - JOUR. T1 - Aspergillus galactomannan antigen in the cerebrospinal fluid of bone marrow transplant recipients with probable cerebral aspergillosis. AU - Viscoli, Claudio. AU - Machetti, Marco. AU - Gazzola, Paola. AU - De Maria, Andrea. AU - Paola, Dimitri. AU - Van Lint, Maria Teresa. AU - Gualandi, Francesca. AU - Truini, Mauro. AU - Bacigalupo, Andrea. PY - 2002. Y1 - 2002. N2 - The Aspergillus galactomannan test was performed on cerebrospinal fluid and serum samples from 5 patients with probable cerebral aspergillosis and from 16 control patients. Cerebrospinal fluid galactomannan levels were significantly higher in aspergillosis patients, and most galactomannan was produced intrathecally. Comparison of serum galactomannan values in pulmonary and cerebral aspergillosis patients showed significant overlapping. Detection of Aspergillus galactomannan in cerebrospinal fluid may be diagnostic of cerebral aspergillosis.. AB - The Aspergillus galactomannan test was performed on cerebrospinal ...
Mannan oligosaccharides (MOSs) are often referred to as one of the potential alternatives for antimicrobial growth promoters. The aim of the paper is to provide a review of mannan oligosaccharide products in relation to their growth promoting effect and mode of action based on the latest publications. We discuss the dietary impact of MOSs on (1) microbial changes, (2) morphological changes of gut tissue and digestibility of nutrients, and (3) immune response of pigs after weaning. Dietary MOSs maintain the intestinal integrity and the digestive and absorptive function of the gut in the post-weaning period. Recent results suggest that MOS enhances the disease resistance in swine by promoting antigen presentation facilitating thereby the shift from an innate to an adaptive immune response. Accordingly, dietary MOS supplementation has a potential growth promoting effect in pigs kept in a poor hygienic environment, while the positive effect of MOS is not observed in healthy pig herds with high hygienic
ASPBA : The Platelia Aspergillus enzyme immunoassay (EIA) is a 1-stage immunoenzymatic sandwich microplate assay that detects galactomannan in bronchoalveolar lavage (BAL) specimens. The assay uses the rat monoclonal antibody EBA-2, which is directed against Aspergillus galactomannan. The monoclonal antibody is used 1) to coat the wells of the microplate and bind the antigen and 2) as the detector antibody in the conjugate reagent (peroxidase-linked monoclonal antibody).   Samples are heat-treated in the presence of EDTA in order to dissociate immune complexes and to precipitate proteins that could possibly interfere with the test. The treated samples and conjugate are added to the wells coated with the monoclonal antibody and incubated. A monoclonal antibody-galactomannan-monoclonal antibody/peroxidase complex is formed in the presence of Aspergillus antigen.   The strips are washed to remove any unbound material, and the substrate solution is added, which will react with the
Complete article is available online.. PDF version is available online.. Objectives: To analyze all known available data to determine whether a mannan oligosaccharide (Bio-Mos; Alltech, Inc, Nicholasville, Kentucky), provided as a feed additive, improves the growth performance of nursery pigs, and what production factors influence the existence or size of that response. Materials and methods: A combined analysis (meta-analysis) was performed using all available, relevant data in a data set based on published and unpublished data. A total of 54 comparisons were made (pigs fed Bio-Mos or no Bio-Mos). The response to Bio-Mos throughout the experimental period was examined with either one or several independent variables in the model. Independent variables included weaning age, length of experiment, dietary Bio-Mos inclusion level, publication status, growth performance during the first nursery stage, antimicrobials in the diet, site of the experiment, and complexity of the first-stage diet. ...
The statistical analysis was performed using the General Linear Model function of the SPSS (2007) Ver. 16.0.1 statistical package (SPSS Inc., Chigaco, IL, USA). The experimentation was based on a factorial model 2 x 2 (MOS x acidifier) or 2 x 2 x 2 (MOS x acidifier x sex). The one-way analysis of variance for the four experimental groups was performed. Also, the two-way analysis of variance and three-way analysis of variance (when applicable) were performed, using as main effects the inclusion of MOS in the feed (two levels), the inclusion of CPr in the feed (two levels) and the sex of the birds (two levels, when applicable). A value of P ,0.050 was considered significant. The Levenes test was applied to test the homogeneity of the variances. Tukeys test was applied to determine statistical differences between the means.. Results and Discussion. The effect of sex and dietary supplementation of MOS and CPr on the body weight is presented in Table 2. During the six weeks of the experimentation ...
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Glucomannan: A weight loss cure or wallet lightener? I recently saw an ad on a website for a supplement called glucomannan. It is sold at GNC and Walmart, and I have thought of trying it. What is it, does it work, is it safe? Overweight and obesity are at near epidemic levels, and…
Looking for your opinion on the appropriate use of β-D-glucan and galactomannan assays for the diagnosis of invasive aspergillosis. I am often asked to interpret two common scenarios: an appropriate host (e.g. Allo-HSCT) with appropriate imaging but negative serum fungal markers, and a less appropriate host (e.g. solid malignancy such as lung cancer), questionable imaging and positive fungal markers. Where do you find these tests to be helpful in clinical practice?
As part of our commitment to providing superior nutrition for horses, Triple Crown adds mannan oligosaccharide, or MOS, to our. ...
... s are polysaccharides consisting of a mannose backbone with galactose side groups (more specifically, a (1-4)-linked
Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structure of LEP-2a was characterized by methylation analysis, FT-IR analysis, and NMR analysis. Results indicated that LEP-2a was a galactomannan with a backbone, composed of 1,2-linked-α-D-Manp, 1,2,6-linked-α-D-Manp, 1,3,4-linked-α-D-Manp, and 1,3-linked-β-D-Galp, which was substituted at O-2, O-3, O-4, and O-6 by branches ...
2BGO: Structure of a Mannan-Specific Family 35 Carbohydrate-Binding Module: Evidence for Significant Conformational Changes Upon Ligand Binding
Learn more about Glucomannan at Grand Strand Medical Center Uses Principal Proposed Uses High Cholesterol Other Proposed...
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Bond Performance and Structural Characterization of Polysaccharide Wood Adhesive Made from Konjac Glucomannan/Chitosan/Polyvinyl Alcohol. Rong Gu,a Baoning Mu,b and Yanpu Yang a. The bond performance and bonding mechanism were evaluated for a Konjac glucomannan (KGM), Chitosan (CS), and polyvinyl alcohol (PVOH) blended wood adhesive. An optimized experimental strategy was used to investigate the effects of the formula parameters of adhesives on the bonding strength of plywood using a Box-Behnken design and response surface methodology (RSM). The microstructure of the blended adhesives was analyzed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). An optimum bonding strength (3.42 ± 0.31 MPa) was achieved with concentrations of KGM, CS, and 10% PVOH of 2.3%, 2.3%, and 5.0%, respectively. There was strong hydrogen bonding between the KGM, CS, and 10% PVOH adhesives and the interface. SEM observations indicated that the blended adhesive exhibited a net-like ...
COMMISSION REGULATION (EU) No 231/2012 of 9 March 2012. Synonyms. Definition Konjac glucomannan is a water-soluble hydrocolloid obtained from Konjac flour by washing with water-containing ethanol. Konjac flour is the unpurified raw product from the tuber of the perennial plant Amorphophallus konjac. The main component is the water-soluble high molecular-weight polysaccharide glucomannan, which consists of D-mannose and D-glucose units at a molar ratio of 1,6:1,0, connected by β(1-4)-glycosidic bonds with a branch at about each 50th or 60th unit. About each 19th sugar residue is acetylated. Einecs:. Chemical name:. Chemical formula:. Molecular weight: 500 000 to 2 000 000. Assay: Total dietary fibre: not less than 95 % on a dry weight basis. Description White to slightly brownish fine particle size, free flowing and odourless powder. Identification. Solubility: Dispersible in hot or cold water forming a highly viscous solution with a pH between 5,0 and 7,0. Solubility is increased by heat and ...
Low prices on Glucomannan! Glucomannan helps control appetite, aid weight loss and promote health*. Glucomannan fiber is produced from konjac root and is capable of absorbing water and dietary fats. Taken with water, glucomannan fiber aids weight loss by creating a comfortable sense of fullness, suppressing your appetite and reducing caloric intake. Studies have shown glucomannan to be a safe and effective way to lose weight, especially if combined with a fat-burning diet and exercise plan.* Glucomannan also minimizes the metabolic impact of meals by slowing the release of food calories into your system.
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EORTC guidelines distinguish between possible, probable and proven fungal infection as infection can only be proven in ,50% of all cases [15]. Uncertainty on our patients aspergillosis diagnosis remained until histologic proof was obtained. While low galactomannan levels have been claimed to be favorable predictors [16], our patients testing was negative despite his complicated clinical picture. It is well known, that galactomannan detection is more sensitive in BAL than in serum. In a series of patients with hematologic malignancies serum galactomannan was only positive in 43% of patients with other mycological evidence of invasive aspergillosis [17]. The sensitivity of galactomannan detection in CSF is less clear. There are some case series indicating, that a positive result is helpful in the diagnosis of cerebral infection [18]. In inconclusive clinical settings, invasive diagnostic procedures need to be adopted. In our case, two organs were biopsied in order to prove the infection and ...
Mannan is a prominent structural component displayed on the cell surface of C. albicans yeast cells (4), and antimannan IgG is present ubiquitously in the general human population (10, 14, 23). Previously, we showed that this naturally occurring antimannan IgG is required for activation of the classical complement pathway when C. albicans is incubated in NHS (40). We now present evidence that this naturally occurring antimannan IgG also regulates activation of the alternative pathway byC. albicans yeast cells. In NHS that is deficient in antimannan antibodies and lacks classical pathway activity as a result of EGTA chelation of serum Ca2+, addition of exogenous antimannan IgG accelerated C3 binding via the alternative pathway in a dose-dependent manner (Fig. 1 and 3). This accelerating effect of antimannan IgG was confirmed in a serum-free complement binding medium that consisted of only purified proteins of the alternative pathway (Fig. 2) as well as in immunofluorescence analysis (Fig. 5). A ...
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Glucomannan fiber is produced from konjac root and is capable of absorbing water and dietary fats. Taken with water, glucomannan fiber aids weight loss by creating a comfortable sense of fullness, suppressing your appetite and reducing caloric intake. Studies have shown glucomannan to be a safe and effective way to lose weight, especially if combined with a fat-burning diet and exercise plan. Glucomannan also minimizes the metabolic impact of meals by slowing the release of food calories into your system. This reduces the insulin response, a key factor in maintaining a balanced and energizing metabolism. As a soluble fiber, glucomannan may help to manage cholesterol levels. As a bulk-forming fiber, glucomannan assists in proper elimination.. ...
1. Passaretti S, Franzoni M, Comin U et al. Action of glucomannans on complaints in patients affected with chronic constipation: a multicentric clinical evaluation. Ital J Gastroenterol 1991; 23(7):421-425. 2. Vuksan V, Sievenpiper J, Owen R et al: Beneficial effects of viscous dietary fiber from Konjac-mannan in subjects with the insulin resistance syndrome. Diabetes Care 2000; 23:9-14. 3. Walsh DE, Yaghoubian V & Behforooz A: Effect of glucomannan on obese patients: a clinical study. Int J Obes 1984; 8(4):289-293. 4. Doi K, Matsuura M, Kawara A et al: Treatment of diabetes with glucomannan (Konjac mannan). Lancet 1979; 1(8123):987-988. 5. Shima K, Tanaka A, Ikegami H et al: Effect of dietary fiber, glucomannan, on absorption of sulfonylurea in man. Horm Metabol Res 1983; 15(1):1-3. 6. Doi K: Effect of konjac fibre (glucomannan) on glucose and lipids. Eur J Clin Nutr 1995; 49(3):S190-S197. 7. Henry D, Mitchell A & Aylward J: Glucomannan and risk of esophageal obstruction. BMJ 1986; ...
The research presented in this thesis explores the potential uses of KGM and the development of KGM containing hydrogels for wound healing applications. The work involved characterization of five different species of Amorphophallus and investigating the biological activity of KGM and different molecular weight fractions of KGM on skin cells. KGM stimulated fibroblast (but not keratinocyte) proliferation and these effect were influenced by the species, Glc:Man ratio, % of glucomannan, molecular weight and the treatments of KGM. KGM also had the ability to maintain fibroblasts and ADMSC viabilities in unchanged medium for 20 days. The involvement of carbohydrate binding receptors on skin cells was also investigated to obtain a better understanding of the biological activity of KGM. KGM also had the potential for cell transportation where examination subjected to shear stress showed positive result. Following these, two sets of KGM hydrogels; crosslinked KGM and interpenetrating network (IPN); ...
A recently published study on rats sheds light on the potential liver health benefits of glucomannans from konjac, a plant native to Asia.
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Clin Infect Dis. 2013 Oct;57(7):1001-4. doi: 10.1093/cid/cit393. Epub 2013 Jun 11. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
Save 50% Fitness Labs - Fiber Diet Glucomannan 500 mg 180 Capsules Fiber Diet Glucomannan 500 mg Bulk-Forming Fiber Stimulant Free Appetite Control & Weight Loss Support* Fiber Supports Weight Loss* Glucomannan, a soluble fiber from Konjac root, was shown in a clinical study to produce 5.5 pounds of weight loss over an 8-week period when subjects took 1 gram (1,000 mg) of glucomannan 1 hour before breakfast, lunch and dinner, while the placebo group gained weight. It reduces appetite by promoting a sense of fullness due to its incredible bulk-forming, water-absorbing powers. The gel it forms in your stomach also helps control the typical insulin and glucose surge from meals. Soluble pectin fibers from apple and grapefruit provide additional health benefits.*
Description Health Benefits Konjac Noodles Shipping Konjac Oat Grape Fruit Fiber Angel Hair Pasta Konjac oat Grape fiber pasta is made from the mixture of konjac fiber, oat fiber and Grape fiber. Konjac oat Grape fiber pasta has very low calories, and high in soluble and insoluble fiber. Konjac glucomannan fiber, whic
The purpose of this study is to investigate the clinical and demographic characteristics and treatment responses of Invasive pulmonary aspergillosis (IPA) in patients with COPD. From January 2006 to December 2011, 71 patients with a positive culture of Aspergillus from lower respiratory tract samples were examined. Of these patients, 11 (15.4%) had IPA with grade 3 or 4 COPD. Aspergillus hyphae was detected in the lung biopsy in three (27.3%) of 11 and defined as proven IPA; the pathological sample was not taken in the other eight (72.7%) patients, and these were defined as probable IPA. Aspergillus isolates were identified as six Aspergillus fumigatus and three Aspergillus niger in nine patients. Patients were diagnosed as IPA 13.8 ± 3.8 days after admission. While five patients required intensive care unit admission, four of them received mechanical ventilation. The most common symptom was dyspnea (n=11, 100%). Positive results of serum galactomannan assays were detected in nine patients ...
β-Mannosidases are exo-acting glycoside hydrolases (GHs) that catalyse the removal of the nonreducing end β-D-mannose from manno-oligosaccharides or mannoside-substituted molecules. They play important roles in fundamental biological processes and also have potential applications in various industries. In this study, the first fungal GH family 5 β-mannosidase (RmMan5B) from Rhizomucor miehei was functionally and structurally characterized. RmMan5B exhibited a much higher activity against manno-oligosaccharides than against p-nitrophenyl β-D-mannopyranoside (pNPM) and had a transglycosylation activity which transferred mannosyl residues to sugars such as fructose. To investigate its substrate specificity and transglycosylation activity, crystal structures of RmMan5B and of its inactive E202A mutant in complex with mannobiose, mannotriose and mannosyl-fructose were determined at resolutions of 1.3, 2.6, 2.0 and 2.4 Å, respectively. In addition, the crystal structure of R. miehei β-mannanase (RmMan5A
Glucomannan is a water-soluble dietary fiber helps to make you feel full after eating when taken with water. Buy Glucomannan supplements plus free UK delivery.
TY - JOUR. T1 - Specificity and diversity of antibodies to Mycobacterium tuberculosis arabinomannan. AU - Navoa, Josephine Anne D.. AU - Laal, Suman. AU - Pirofski, Liise Anne. AU - McLean, Gary R.. AU - Dai, Zhongdong. AU - Robbins, John B.. AU - Schneerson, Rachel. AU - Casadevall, Arturo. AU - Glatman-Freedman, Aharona. PY - 2003/1/1. Y1 - 2003/1/1. N2 - Arabinomannan (AM) is a polysaccharide antigen of the mycobacterial capsule. However, it is uncertain whether AM constitutes an immunologically distinct fraction of Mycobacterium tuberculosis. In this study, we analyzed the repertoire and specificity of antibodies to AM by using AM-binding murine monoclonal antibodies (MAbs) and human serum samples. Murine MAbs were found to be diverse in their specificity to AM and cross-reactivity with other arabinose-containing mycobacterial polysaccharides, with MAb 9d8 binding exclusively to AM. Human antibodies to AM were detected in serum samples from patients with pulmonary tuberculosis (TB), as well ...
... is a dietary supplement marketed as a weight loss aid. It is not a medically prescribed weight loss aid.. How It Works Natures Science, the manufacturer of Lipothin, claims that the supplement is made from "pure and natural" Amorphoallus konjac. Natures Science describes konjac as a water-soluble fiber that can reduce weight.. Konjac is a perennial plant native to Eastern Asian countries like China and Japan. The corm of the plant is used to make popular foods like yam cake and fruit jelly. Konjac corm, when dried, contains glucomannan, a water-soluble polysaccharide considered a dietary fiber. Powdered Glucommanan is included in foods and various traditional medicines and dietary supplements.. Glucomannan is believed to treat conditions such as constipation, type 2 diabetes, high blood sugar, high cholesterol and obesity. In traditional Chinese medicine, konjac-derived Glucomannan is used for tumor suppression, blood statis, detoxification, and phlegm liquefaction.. Because konjac ...
Konjac Root Glucomannan 1000 mg helps maintain cholesterol levels already within a normal range. Shop Puritans Pride for high quality & great value.
Glucomannan, how it can help you lose weight. Glucomannan is a natural fiber extracted from the roots of the konjac plant. How does it work for weight loss
Glucomannan supplements may help reduce cholesterol but less likely glucose levels in diabetics. Insufficient evidence about the effect on weigh loss.
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The search for new and more efficient glycoside hydrolases (GH) has intensified over the last few years due to a need for such enzymes in the biofuel industry. Man5B, a cytoplasmic enzyme of the glycoside hydrolase family 5, has been shown to cleave manno- and gluco-oligosaccharides into mono- and disaccharides for subsequent metabolism[5]. Experimental assays show that Man5B acts more efficiently on manno-oligosaccharides than on gluco-oligosaccharides, however the mechanism for this behavior was not clear. We have performed a molecular dynamics study to identify this mechanism and to elucidate which amino acid residues are controlling enzymatic efficiency. The insights gained from these studies are critical to the development of more efficient enzymes through rational targeting of residues for site-directed mutagenesis.. The molecular dynamics simulations yielded surprising results in that they showed Man5B to bind cellohexaose nearly as tightly as mannohexaose, as shown in Figure 3A. The RMSD ...
Yeepet Web Store Pet Naturals Quick Relief For Dogs [0700967.004] - QUICK RELIEF is a highly palatable, fast-acting formula for dogs with gastrointestinal conditions associated with microflora imbalances, garbage gut, food sensitivities, stress, age and traveling. It contains a specialized yeast extract (Saccharomyces cerevisiae) that is rich in Mannan Oligosaccharides (MOS) along with Direct Fed Microbials, Digestive Enzymes and B Vitamins
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