A promising avenue of research in materials science is to follow the strategies used by Mother Nature to fabricate ornate hierarchical structures as exemplified by organisms such as diatoms, sponges and magnetotactic bacteria. Some of the strategies used in the biological world to create functional inorganic materials may well have practical implications in the world of nanomaterials. The aim of our work is to examine the synthetic of magnetite nanoparticles under different conditions to show the influence in magnetic properties of magnetite nanoparticles. Magnetospirillum strain AMB-1 was used in this study in order to produce magnetite nanoparticles. Magnetite nanoparticles of average size~47 nm were obtained. The magnetic properties of magnetite nanoparticles under different incubation temperature were examined and a small influence in magnetic properties of magnetite nanoparticles was indicated.
In this study, magnetic iron oxide nanoparticles (Fe3O4) with the size range of 20-30 nm were prepared by the modified controlled chemical co-precipitation method from the solution of ferrous/ferric mixed salt-solution in alkaline medium. In this process polyethylene glycol was used as a surfactant to prevent the solution from agglomeration. The prepared magnetic nanoparticles were characterized by X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM) and vibrating-sample magnetometer (VSM). XRD image indicates the sole existence of inverse cubic spinel phase of magnetic iron oxide nanoparticles (Fe3O4). SEM image show that the dimension of magnetic iron oxide nanoparticles (Fe3O4) is about 24 nm. VSM patterns demonstrate superparamagnetic properties of magnetic nanoparticles.
TY - JOUR. T1 - Cellular uptake of folate-conjugated lipophilic superparamagnetic iron oxide nanoparticles. AU - Woo, Kyoungja. AU - Moon, Jihyung. AU - Choi, Kyu Sil. AU - Seong, Tae Yeon. AU - Yoon, Kwon Ha. PY - 2009/5. Y1 - 2009/5. N2 - We prepared five folate-conjugated lipophilic superparamagnetic iron oxide nanoparticles (F5-Liposuperparamagnetic iron oxide nanoparticles(SPIONs), 5.5 and 11 nm) and investigated their cellular uptake with KB cells, which is one of the representative folate-receptor over-expressing human epidermoid carcinoma cells, using MRI. The cellular uptake tests with the respective 5.5 and 11 nm F5-LipoSPIONs at a fixed particle concentration showed appreciable amount of receptor-mediated uptakes and the specificity was higher in 5.5 nm SPIONs, due to its higher folic acid (FA) density, without inhibition. However, the numbers of the particles taken up under FA inhibition were similar, irrespective of their sizes.. AB - We prepared five folate-conjugated lipophilic ...
In recent years, nanotechnology has emerged as one of the most promising fields for biomedical applications and healthcare. The use of nanoparticles has earned much attention due to their small size, high surface area to volume ratio along with surface charge, and their optical and semiconducting properties. Superparamagnetic iron oxide nanoparticles (SPIONs), which show superparmagnetism, have been reported to be reliable and safe for many biological applications.In the present study, 10nm SPION were loaded with anticancer drug, Docetaxel. Docetaxel conjugation to SPION was confirmed by FTIR, UV-vis spectroscopy and gel electrophoresis. SPIONs uptake by cells was investigated using Prussian blue dye staining.In vitro drug release was studied which showed 55% drug release in 24 h. Drug loaded SPION were then tested on Mammary tumor cells (4T1) and showed 40% cell viability with 0.1M Docetaxel conjugated to SPION. Our results shows that Docetaxel-SPIONs can reduce the amount of required anticancer drug
Page contains details about c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
A method and structure for making magnetite nanoparticle materials by mixing iron salt with alcohol, carboxylic acid and amine in an organic solvent and heating the mixture to 200-360 C is described. The size of the particles can be controlled either by changing the iron salt to acid/amine ratio or by coating small nanoparticles with more iron oxide. Magnetite nanoparticles in the size ranging from 2 nm to 20 nm with a narrow size distribution are obtained with the invention. The invention can be readily extended to other iron oxide based nanoparticle materials, including M Fe2O4 (M=Co, Ni, Cu, Zn, Cr, Ti, Ba, Mg) nanomaterials, and iron oxide coated nanoparticle materials. The invention also leads to the synthesis of iron sulfide based nanoparticle materials by replacing alcohol with thiol in the reaction mixture. The magnetite nanoparticles can be oxidized to γ-Fe2O3, or α-Fe2O3, or can be reduced to bcc-Fe nanoparticles, while iron oxide based materials can be used to make binary iron based
We demonstrate that fatty acid modified iron oxide prolonged retention of the contrast agent in the polymer matrix during degradative release of drug. Antibody-fatty acid surface modification facilitated cellular targeting and subsequent internalization in cells while inducing clustering of encapsulated fatty-acid modified superparamagnetic iron oxide during particle formulation. This induced clustered confinement led to an aggregation within the nanoparticle and, hence, higher transverse relaxivity, r2, (294 mM−1 s−1) compared with nanoparticles without fatty-acid ligands (160 mM−1 s−1) and higher than commercially available superparamagnetic iron oxide nanoparticles (89 mM−1 s−1). ...
The study is evaluating Magnetic Resonance Imaging Using Ultrasmall Superparamagnetic Particles of Iron Oxide in Patients Under Surveillance for Abdominal
Here we report new work on the synthesis and an electron microscopy study of the earliest known magnetic material, magnetite (Fe$_{3}$O$_{4}$). We have synthesized a variety of magnetite nanoparticles which appear to have biogenic signatures and could give insights into how the nanomagnetite in biological systems forms, and how they may be associated with Alzheimers disease. We have also synthesized mesoporous magnetite nanoparticles which have potential use in the targeted drug delivery ...
Tracking vaccine components from the site of injection to their destination in lymphatic tissue, and simultaneously monitoring immune effects, sheds light on the influence of vaccine components on particle and immune cell trafficking and therapeutic efficacy. In this study, we create a hybrid particle vaccine platform comprised of porous silicon (pSi) and superparamagnetic iron oxide nanoparticles (SPIONs). The impact of nanoparticle size and mode of presentation on magnetic resonance contrast enhancement are examined. SPION-enhanced relaxivity increased as the core diameter of the nanoparticle increased, while encapsulation of SPIONs within a pSi matrix had only minor effects on T2 and no significant effect on T2* relaxation. Following intravenous injection of single and hybrid particles, there was an increase in negative contrast in the spleen, with changes in contrast being slightly greater for free compared to silicon encapsulated SPIONs. Incubation of bone marrow-derived dendritic cells (BMDC) with
In of this studying the green synthesis of Iron Oxide nanoparticles (Fe|sub|2|/sub|O|sub|3|/sub| NPs) with Celery stalks and green tea leaves extract were used. The fresh suspension of plant extracts ware green- brown in color. However, after acting of FeNO|sub|3|/sub| within 20min, the suspension showed the change in color and turned dark brown after 4 hours of incubation at room temperature. Formation of Iron oxide nanoparticles was confirmed using X-ray is spectral analysis and showed the characteristic Bragg peaks of (111) to green tea extract and (111) to celery extract, plant of the face center cubic (FCC) Iron Oxide nanoparticles. The scanning electron microscope (SEM) Iron oxide nanoparticles see small particles and rode. The synthesized Fe|sub|2|/sub|O|sub|3|/sub| NPs colloidal solution has shown better antibacterial activity against both Gram-positive and Gram-negative bacterial strains. The diameters of the inhibition zones of Fe|sub|2|/sub|O|sub|3|/sub| NPs against the bacterial strains were
Targeted radioisotope-guided sentinel lymph node dissection (sLND) has shown high diagnostic accuracy in prostate cancer (PCa). To overcome the downsides of the radioactive tracers, magnetometer-guided sLND using superparamagnetic iron oxide nanoparticles (SPIONs) was successfully applied in PCa. This prospective study (SentiMag Pro II, DRKS00007671) determined the diagnostic accuracy of magnetometer-guided sLND in intermediate- and high-risk PCa. Fifty intermediate- or high-risk PCa patients (PSA≥10 ng/ml and/or Gleason score ≥7; median PSA 10.8 ng/ml, IQR 7.4–19.2 ng/ml) were enrolled. After intraprostatic SPIONs injection a day earlier, patients underwent magnetometer-guided sLND and eLND, followed by radical prostatectomy. SLNs were detected in vivo and in ex vivo samples. Diagnostic accuracy of sLND was assessed using eLND as the reference. SLNs were detected in all patients (detection rate 100%), with 447 SLNs (median 9, IQR 6–12) being identified and 966 LNs (median 18, IQR 15
Purpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide ([email protected]) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 ([email protected]), dextran ([email protected]), or bare SPIO in mammalian cell lines. Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7-15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbeccos modified Eagles medium (DMEM) with 4.5 μg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO
Rapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching. Once thrombin was introduced, the CY3-aptamer formed a G-quartet structure and combined with thrombin, which resulted in the CY3-aptamer being separated from the magnetic nanoparticles and restoration of fluorescence. This proposed assay took advantage of binding affinity between the CY3-aptamer and thrombin for specificity, and bare magnetic nanoparticles for fluorescence quenching. The fluorescence signal had a good linear relationship ...
We have demonstrated that the Verwey transition, which is highly sensitive to impurities, survives in anisotropic Gd-doped magnetite nanoparticles. Transmission electron microscopy analysis shows that the nanoparticles are uniformly distributed. X-ray photoelectron spectroscopy and EDS mapping analysis confi
Magnetically-Guided Nanoparticle Drug Delivery Seth Baker, RET Fellow 2011 Percy Julian Middle School RET Mentor: Prof. Andreas A. Linninger Chicago Science Teacher Research (CSTR) Program - NSF-RET 2011. Introduction. Magnetite Nanoparticles. Superparamagnetic Properties. Motivation...
Arsenic (As) contamination in groundwater is a great environmental health concern and is often the result of contact between groundwater and arsenic-containing rocks or sediments and from variation of pH and redox potentials in the subsurface. In the past decade, magnetite nanoparticles (MNPs) have been shown to have high adsorption activity towards As. Alerted by the reported cytotoxicity of 5–12 nm MNP, we studied the adsorption behavior of 1.15 nm MNP and a MNP composite (MNPC), MNPs interlinked by silane coupling agents. With an initial concentration of As at 25 mg L−1, MNPs exhibited high adsorption capacity for As(V) and As (III), 206.9 mg·g−1 and 168.6 mg·g−1 under anaerobic conditions, respectively, and 109.9 mg·g−1 and 108.6 mg·g−1 under aerobic conditions, respectively. Under aerobic conditions, MNPC achieved even higher adsorption capacity than MNP, 165.1 mg·g−1 on As(V) and 157.9 mg·g−1 on As(III). For
Preferred Name: Ferumoxtran-10 Definition: A synthetic ultrasmall superparamagnetic iron oxide composed of dextran-coated iron oxide nanoparticles (also known as ultrasmall particulate iron oxides or USPIO). Ferumoxtran-10, which accumulates in non-cancerous lymphatic tissue, is used as a molecular resonance imaging (MRI) contrast agent. (NCI04) NCI-GLOSS Definition: A substance being studied as a way of improving magnetic resonance imaging (MRI) in diagnosing cancer and finding lymph nodes to which cancer has spread. Ferumoxtran-10 is made of nanoparticles (ultrasmall pieces) of iron oxide coated with dextran (a type of sugar). It is injected into the blood of the patient and the particles collect in lymph nodes, liver, spleen, or brain tissue where they can be seen using MRI. Ferumoxtran-10 later breaks down and passes from the body in urine. Display Name: Ferumoxtran-10 Label: Ferumoxtran-10 NCI Thesaurus Code: C38140 (Search for linked caDSR metadata) (search value sets) NCI Metathesaurus ...
Compared with previous 1H MRI approaches for visualizing the infiltration of immunocompetent cells into inflamed areas by use of superparamagnetic iron oxide particles, the method presented here has the advantage of a direct positive detection of the tagging agent and therefore has the potential to work also in tissues that generally appear very dark in 1H MRI such as the lungs. Although techniques have recently been described to image superparamagnetic iron oxide particles with a bright contrast,18 the physical basis of detection is still the disturbance of the regional magnetic field by these particles. Therefore, it often remains difficult to unequivocally assign alterations in local contrast to accumulating superparamagnetic iron oxide particles. Furthermore, iron-based contrast agents are readily metabolized, whereas the fluorinated crown ether used in this study is biologically inert and cannot easily be degraded. The reason is the very stable C-F bond and the dense electron cloud of the ...
Studying the effect of particle size and coating type on the blood kinetics of superparamagnetic iron oxide nanoparticles Farnoosh Roohi, Jessica Lohrke, Andreas Ide, Gunnar Schütz, Katrin DasslerMR and CT Contrast Media Research, Bayer Pharma AG, Berlin, GermanyPurpose: Magnetic resonance imaging (MRI), one of the most powerful imaging techniques available, usually requires the use of an on-demand designed contrast agent to fully exploit its potential. The blood kinetics of the contrast agent represent an important factor that needs to be considered depending on the objective of the medical examination. For particulate contrast agents, such as superparamagnetic iron oxide nanoparticles (SPIOs), the key parameters are particle size and characteristics of the coating material. In this study we analyzed the effect of these two properties independently and systematically on the magnetic behavior and blood half-life of SPIOs.Methods: Eleven different SPIOs were synthesized for this study. In the first set
In the past few years, the use of nanostructured materials in medical applications hasdramatically increased, both in the research phase and for clinical purposes, due to thepeculiar properties and the ability of such materials to interact at a similar scale withbiological entities. In this thesis, we developed tailored magnetic multifunctionalnanoparticles for diagnostic and therapeutic applications, such as detection ofbiomolecules, simultaneous enhanced magnetic resonance imaging (MRI), fluorescentvisualization and controlled drug release.For sensitive and selective detection of specific biomolecules, thermally blocked ironoxide nanoparticles with tailored magnetic properties were developed. The formation ofsuch nanoparticles has been studied both in terms of size and magnetic behavior in liquidsuspension or in polymer matrixes. These particles with narrow size distribution (averagediameter of 19 nm) were surface functionalized by antigen molecules and were used forthe detection of Brucella ...
Mentors: Anderson, Hilt. Iron oxide nanoparticles have the ability to enhance the production of reactive oxygen species (ROS) within cells through catalyzing the Haber-Weiss reaction (Fenton chemistry) which produces the hydroxyl radical. Cancer cells are more susceptible to oxidative insults compared to normal cells due to fast cell proliferation and metabolism so additional ROS stress induced by exogenous agents can overwhelm the relatively low antioxidant capacity and disrupt the redox homeostasis inside cancer cells leading to selective tumor cell toxicity. Iron oxide nanoparticles have been previously studied due to their multitude of biological applications, inherent biocompatibility, magnetic properties, and lack of protein adsorption after proper coating. Therefore, iron oxide nanoparticles coated with dextran will be used to improve the efficacy of radiation by enhancing the intracellular ROS production.. Due to the half-life of the hydroxyl radical being on the order of a nanosecond, ...
A method to prepare amyloid-like fibrils functionalized with magnetic nanoparticles has been developed. The amyloid-like fibrils are prepared in a two step procedure, where insulin and magnetic nanoparticles are mixed simply by grinding in the solid state, resulting in a water soluble hybrid material. When the hybrid material is heated in aqueous acid, the insulin/nanoparticle hybrid material self assembles to form amyloid-like fibrils incorporating the magnetic nanoparticles. This results in magnetically labeled amyloid-like fibrils which has been characterized by Transmission Electron Microscopy (TEM) and electron tomography. The influence of the aggregation process on proton relaxivity is investigated. The prepared materials have potential uses in a range of bio-imaging applications.. ...
Iron oxide nanoparticles are a promising resource for solving some of todays most pressing global challenges in the developed and developing world, including the removal of toxins from drinking water resources, treatment of mining wastes, and remediation of groundwater contaminated by industrial activity. There is still much to be understood, however, about the reactivity of iron oxide nanoparticles in actual groundwater systems, where mineralogy and solution conditions are complex and variable over time. In this thesis, iron oxide nanoparticle reactivity was measured as a function of environmental variables, including pH, ionic strength, and the presence of organic matter or secondary mineral phases. The chosen variables simulate severe and impacted environments, such as pesticide-polluted groundwater and acid mine drainage. Additionally, kinetic studies paired with complementary solid-state characterization were used to elucidate evolving reactivity (changes in reactivity and iron oxide ...
The fast development of the nanotechnology, and the use of this technology to develop new products to help in diagnosis diseases, drugs and treatments for many diseases is increasing. Some authors have been warning about the nanoparticles use, because the real affect, toxic or not, still unknown. Iron oxide nanoparticles have been used in contrast liquid for magnetic resonance imaging, cancer treatment, among others. The aim of this work was to analyze the possible effects of chronic treatment with these nanoparticles in adults rats. The animals were distributed in 3 groups: Control (saline 0.9% - 0.1 mL/100 of body weight), nFe 0.3 (magnetic iron oxide nanoparticles 0.3 mg/kg BW) and nFe 0.6 (magnetic iron oxide nanoparticles 0.6 mg/kg BW). The animals were treated by gastric gavage during 8 weeks, 5 days per week. The body weight of the animals from the nFe 0.6 group decreased when compared to animals from the control group, and the BW of the animals from nFe 0.3 group was not different from ...
We report a synthesis of magnetic nanoparticles chemically immobilized onto reduced graphene oxide sheets (referred to as rGO-Fe3O4 NPs) as a gas and vapor sensing platform with precisely designed particle size of 5, 10 and 20 nm to explore their influence of particle size on sensing performance. The rGO-Fe3O4 NP sensors have been investigated their responses to different gases and volatile organic compounds (VOCs) at part-per-million (ppm) levels. Results show that the Fe3O4 NPs with smaller size (5 and 10 nm) on the rGO surface led to a lower sensitivity, while particles of a size of 20 nm have a significant enhancement of sensitivity compared to the bare rGO sensor. The rGO-Fe3O4 NP20 sensor can detect trace amounts of NO2 gas and ethanol vapor at the 1 ppm and is highly selective to the NO2 and ethanol among other tested gases and VOCs, respectively. The particle size causes different distribution behaviour of NPs over rGO surface and interspaced between them, which results in deceased or ...
Anti-angiogenic therapy is efficacious in metastatic renal cell carcinoma (mRCC). However, the ability of anti-angiogenic drugs to delay tumor progression and extend survival is limited, due to either innate or acquired drug resistance. Furthermore, there are currently no validated biomarkers that predict which mRCC patients will benefit from anti-angiogenic therapy. Here we exploit susceptibility contrast magnetic resonance imaging (SC-MRI) using intravascular ultrasmall superparamagnetic iron oxide particles to quantify and evaluate tumor fractional blood volume (fBV) as a non-invasive imaging biomarker of response to the anti-angiogenic drug sunitinib. We also interrogate the vascular phenotype of RCC xenografts exhibiting acquired resistance to sunitinib. SC-MRI of 786-0 xenografts prior to and two weeks after daily treatment with 40mg/kg sunitinib revealed a 71% (p,0.01) reduction in fBV in the absence of any change in tumor volume. This response was associated with significantly lower ...
Limited research has suggested iron oxide nanoparticles (FeNP) have an inhibitory effect against several different genera of bacteria: Staphylococcus, Bacillus and Pseudomonas spp. In this study we looked at the effect of three different sets of Fe3O4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. Two of the tested NPs were SPIONs (Superparamagnetic Iron Oxide Nanoparticles). Exposure of cells to the SPIONs at concentrations up to 200 μg/ml resulted in an increase in biofilm biomass by 16 h under static conditions and a corresponding increase in cell density in the bulk liquid. In contrast, these biofilms had decreased levels of extracellular DNA (eDNA). Fe(II) levels in the supernatants of biofilms formed in the presence of FeNPs exceeded 100 μM compared with 20 μM in control media without cells. Spent cell supernatants had little effect on Fe(II) levels. Cells also had an effect on the aggregation behavior of these nanoparticles. SPIONs incubated with cells
TY - JOUR. T1 - Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model. AU - Park, Ji Won. AU - Ku, Sook Hee. AU - Moon, Hyung Ho. AU - Lee, Minhyung. AU - Choi, Donghoon. AU - Yang, Jaemoon. AU - Huh, Yong Min. AU - Jeong, Ji Hoon. AU - Park, Tae Gwan. AU - Mok, Hyejung. AU - Kim, Sun Hwa. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered MSCs are also clearly visualized via MR ...
Although doxorubicin (DOX) is an effective anti-cancer drug with cytotoxicity in a variety of different tumors, its effectiveness in treating glioblastoma multiforme (GBM) is constrained by insufficient penetration across the blood-brain barrier (BBB). In this study, biocompatible magnetic iron oxide nanoparticles (IONPs) stabilized with trimethoxysilylpropyl-ethylenediamine triacetic acid (EDT) were developed as a carrier of DOX for GBM chemotherapy. The DOX-loaded EDT-IONPs (DOX-EDT-IONPs) released DOX within 4 days with the capability of an accelerated release in acidic microenvironments. The DOX-loaded EDT-IONPs (DOX-EDT-IONPs) demonstrated an efficient uptake in mouse brain-derived microvessel endothelial, bEnd.3, Madin-Darby canine kidney transfected with multi-drug resistant protein 1 (MDCK-MDR1), and human U251 GBM cells. The DOX-EDT-IONPs could augment DOXs uptake in U251 cells by 2.8-fold and significantly inhibited U251 cell proliferation. Moreover, the DOX-EDT-IONPs were found to be
Superparamagnetic iron oxide nanoparticles (SPIO) have been synthesized and explored for use as carriers of various nanoadjuvants via loading into dendritic cells (DCs). In our study, homogeneous and superparamagnetic nanoparticles are susceptible to internalization by DCs and SPIO-pulsed DCs showed excellent biocompatibility and capacity for ovalbumin (OVA) cross-presentation. Herein, we found that SPIO-loaded DCs can promote the maturation and migration of DCs in vitro. SPIO coated with 3-aminopropyltrimethoxysilane (APTS) and meso-2,3-dimercaptosuccinic acid (DMSA), which present positive and negative charges, respectively, were prepared. We aimed to investigate whether the surface charge of SPIO can affect the antigen cross-presentation of the DCs. Additionally, the formation of interleukin-1β (IL-1β) was examined after treatment with oppositely charged SPIO to identify the nanoadjuvants mechanism. In conclusion, our results suggest that SPIO are biocompatible and can induce the migration of DCs
A. D. L. Zerda, C. Zavaleta, S. Keren, S. Vaithilingam, S. Bodapati, Z. Liu, J. Levi, B. R. Smith, T. J. Ma, O. Oralkan, Z. Cheng, X. Chen, H. Dai, B. T. Khuri-Yakub, and S. S. Gambhir, Nat. Nanotech. 3, 557-562 (2008 ...
June 2017 Congratulations to Shoronia Cross, who won the 2017 David J. Simkin Award in Physical Chemistry! Congratulations to Serene Bayram, who won an FRQNT Postdoctoral Fellowship! May 2017 Romane Thiboutot-Martin joins the group to work on TMV templated gold nanorings for the summer--welcome! Hannah Sragivicz joins the group to work on iron oxide nanoparticles for May and June--welcome! Alice Kahan joins the group to work on gold nanorod synthesis for the summer with a Summer Undergraduate Research Award--congratulations and welcome! Janet Tang joins the group to work on self-assembly of gold nanorods for the summer with a Summer Undergraduate Research Award--congratulations and welcome! Yifan Ling joins the group to work on iron oxide nanoparticles for the summer--welcome! Dr. Blum gives a talk on Nanoparticles by design: controlling the surface chemistry of superparamagnetic iron oxide nanoparticles at Surface Canada in Montreal. Dr. Blum gives a talk on Nanoring Formation via In-situ
high quality and high precision iron oxide nanoparticles, GERHOLD must be your best choice as we offer well processed iron oxide nanoparticles. In order to meet your requirements, we also offer customized service.
Completely water dispersible and highly monodispersed superparamagnetic iron oxide nanoparticles (SPIONs) were prepared. The surface of SPIONs was modified with dual-crosslinked amine activated dextran (AMD) and chemical cleavage of AMD on SPIONs was carried out by ethylenediamine hydrochloride (EDA). Transmission electron microscopy (TEM) revealed that individual SPIONs were completely separated in water and the average diameter of resulting nanoparticles was 4.4 nm.. ...
Using magnetic nanoparticles to absorb alternating magnetic field energy as a method of generating localized hyperthermia has been shown to be a potential cancer treatment. This report demonstrates a system that uses tumor homing cells to actively carry iron/iron oxide nanoparticles into tumor tissue for alternating magnetic field treatment. Paramagnetic iron/iron oxide nanoparticles were synthesized and loaded into RAW264.7 cells (mouse monocyte/macrophage-like cells), which have been shown to be tumor homing cells. A murine model of disseminated peritoneal pancreatic cancer was then generated by intraperitoneal injection of Pan02 cells. After tumor development, monocyte/macrophage-like cells loaded with iron/iron oxide nanoparticles were injected intraperitoneally and allowed to migrate into the tumor. Three days after injection, mice were exposed to an alternating magnetic field for 20 minutes to cause the cell-delivered nanoparticles to generate heat. This treatment regimen was repeated ...
The magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) have made them useful tools in a variety of applications ranging from drug delivery to magnetorheologic valving in microfluidic systems. The structure of SPIONs is typically characterized by methods such as XRD, XAFS, SEM and high resolution TEM while the SPION magnetic properties are evaluated relative to bulk values using SQUID. These methods characterize the state of the nanoparticles; however, the methods provide limited information about the behavior of the particles in solution. Here we demonstrate that electron paramagnetic resonance (EPR) reveals the solution behavior of 13 nm SPIONs suspended in a methanol:glycerol solution as well as environmental changes around the SPIONs. For example, characteristic temperature-dependent changes in the EPR spectra detail orientation changes of the SPIONs. The data presents a new opportunity for observing the in vitro, in vivo, and solution behavior of magnetite SPIONs.
Superparamagnetic iron oxide nanoparticles are one of the most prominent agents used in theranostic applications, with MRI imaging the main application assessed. The biomolecular interface formed on the surface of a nanoparticle in a biological medium determines its behaviour in vitro and in vivo. In this study, we have compared the formation of the protein corona on highly monodisperse iron oxide nanoparticles with two different coatings, dimercaptosuccinic acid (DMSA), and after conjugation, with a bifunctional polyethylene glycol (PEG)-derived molecule (2000 Da) in the presence of Wistar rat plasma. The protein fingerprints around the nanoparticles were analysed in an extensive proteomic study. The results presented in this work indicate that the composition of the protein corona is very difficult to predict. Proteins from different functional categories-cell components, lipoproteins, complement, coagulation, immunoglobulins, enzymes and transport proteins-were identified in all samples with ...
Recent progress in nanotechnology and electrochemical methods can be applied to fine control of the size، crystal structure، and surface properties of iron oxide nanoparticles. Here we appliedcathodic electrochemical deposition (CED) as an efficient and effective tactic for synthesisand double coating of surface of superparamagnetic iron oxide nanoparticles (SPIONs). In first step، bare Fe3O4 nanoparticles were prepared by CED method using a molar ratio of Fe3+:Fe2+ of 2:1. In the next step، the surface of nanoparticles was double coated with dextran (DEX) and polyethylene glycol (PEG) during the CED procedure، and PEG/DEX coated SPIONs were obtained. The prepared NPs were characterized using powderX-ray diffraction (XRD)، Fourier transform infrared spectroscopy (FTIR)، thermogravimetric analysis (TGA)، dynamic light scattering (DLS)، vibrating sample magnetometer (VSM) and Field-emission scanning and transmission electron microscopy (FE-SEM and TEM). The XRD results confirmed that both ...
A comprehensive literatures update of clinical researches of superparamagnetic resonance iron oxide nanoparticles for magnetic resonance imaging
Recent Advances in Synthesis and Biomedical Applications of Magnetic Nanoparticles: Magnetic Nanoparticles for Biomedical Applications: 10.4018/978-1-5225-3158-6.ch060: Magnetic nanoparticles due to their unique magnetic phenomenon, are gaining immense interest due to the utilization of these properties for a wide variety of
TY - JOUR. T1 - Ex vivo magnetofection with magnetic nanoparticles. T2 - A novel platform for nonviral tissue engineering. AU - Yang, Shieh Yueh. AU - Sun, Jui Sheng. AU - Liu, Cheng Heng. AU - Tsuang, Yang Hwei. AU - Chen, Li Ting. AU - Hong, Chin Yih. AU - Yang, Hong Chang. AU - Horng, Herng Er. PY - 2008/3. Y1 - 2008/3. N2 - Several methods have been described to introduce DNA expression vectors into mammalian cells both in vitro and in vivo. Each system has benefits and limitations, and to date there is still no ideal method for gene transfer. In this study, we introduced a novel method of gene transfer by using Fe 3O4 nanoparticles. The magnetic nanoparticles composed of Fe3O4, and the transfected genes used are Lac Z and enhanced green fluorescence protein gene (EGFG). Four different groups of preparations included in this study were homemade liposome-enveloped EGFP-DNA/Fe3O4, homemade liposome EGFP-DNA gene without magnetic Fe3O4 nanoparticles, lipofectamine 2000-enveloped EGFP-DNA, and ...
Toxicity testing the rapidly growing number of nanomaterials requires large scale use of in vitro systems under the presumption that these systems are sufficiently predictive or descriptive of responses in in vivo systems for effective use in hazard ranking. We hypothesized that improved relationships between in vitro and in vivo models of experimental toxicology for nanomaterials would result from placing response data in vitro and in vivo on the same dose scale, the amount of material associated with cells. Balb/c mice were exposed nose-only to an aerosol (68.6 nm CMD, 19.9 mg/m3, 4 hours) generated from of 12.8 nm superparamagnetic iron oxide particles (SPIO). Target cell doses were calculated, histological evaluations conducted, and biomarkers of response were identified by global transcriptomics. Representative murine epithelial and macrophage cell types were exposed in vitro to the same material in liquid suspension for four hours and levels of nanoparticle regulated cytokine transcripts
Toxicity testing the rapidly growing number of nanomaterials requires large scale use of in vitro systems under the presumption that these systems are sufficiently predictive or descriptive of responses in in vivo systems for effective use in hazard ranking. We hypothesized that improved relationships between in vitro and in vivo models of experimental toxicology for nanomaterials would result from placing response data in vitro and in vivo on the same dose scale, the amount of material associated with cells. Balb/c mice were exposed nose-only to an aerosol (68.6 nm CMD, 19.9 mg/m3, 4 hours) generated from of 12.8 nm superparamagnetic iron oxide particles (SPIO). Target cell doses were calculated, histological evaluations conducted, and biomarkers of response were identified by global transcriptomics. Representative murine epithelial and macrophage cell types were exposed in vitro to the same material in liquid suspension for four hours and levels of nanoparticle regulated cytokine transcripts
Hybrid nanogels, composed of thermoresponsive polymers and superparamagnetic nanoparticles (MNPs) are attractive nanocarriers for biomedical applications, being able - as polymer matrix - to uptake and release high quantities of chemotherapeutic agents and - as magnetic nanoparticles - to heat when exposed to an alternative magnetic field (AMF), better known as magnetic hyperthermia. Herein, biocompatible, pH-, magnetic-and thermo-responsive nanogels, based on oligo (ethylene glycol) methacrylate monomers 2 (OEGMAs) and methacrylic acid co-monomer (MAA) were prepared by conventional precipitation radical co-polymerization in water, post-assembled by complexation with iron oxide magnetic nanoparticles (MNPs) of maghemite (-Fe 2 O 3) and loaded with an anticancer drug (doxorubicin - DOX), for remotely controlled drug release by hot-spot , as an athermal magnetic hyperthermia strategy against cancer. These nanogels, noted MagNanoGels, with a hydrodynamic diameter from 328 to 460 nm, as a function of
Adipose-derived stem cells (ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide (SPIO) nanoparticles are critical for magnetic resonance (MR) tracking of implanted cells. Hypoxia is a powerful stimulus for angiogenic activity of ASCs. In this study, we investigated whether therapeutic potency could be enhanced by implantation of hypoxia-preconditioned SPIO-labeled ASCs (SPIOASCs) into the infarcted myocardium. ASCs and SPIOASCs were cultured under 2% O2 (hypoxia) or 95% air (normoxia). Cells were intramyocardially injected into the infarcted myocardium after 48-h culture. We found that hypoxia culture increased the mRNA expression of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in ASCs and SPIOASCs. The VEGF protein in the conditioned medium was significantly higher in hypoxic ASCs and SPIOASCs than in normoxic ASCs and SPIOASCs. The capillary density and left ventricular contractile function in the
From Oct. 7: We identify the abundant presence in the human brain of magnetite nanoparticles that match precisely the high-temperature magnetite nanospheres, formed by combustion and/or frictionderived heating, which are prolific in urban, airborne particulate matter. Because many of the airborne magnetite pollution particles are ,200 nm in diameter,they can enter the brain directly through the olfactory nerve and by crossing the damaged olfactory unit. This discovery is important because nanoscale magnetite can respond to external magnetic fields, and is toxic to the brain, being implicated in production of damaging reactive oxygen species. Because enhanced ROS production is causally linked to neurodegenerative diseases such as Alzheimers disease, exposure to such airborne PM-derived magnetite nanoparticles might need to be examined as a possible hazard to human health. READ MORE ...
Radar absorbing materials, i.e. magnetite (Fe3O4) coated carbon fibers (MCCFs) were fabricated by electro-deposition technique. Black-colored single spinel phase Fe3O4 nanoparticles was easily synthesized by hydrothermal method using reduction of a Fe (III) - Triethanolamine complex in an aqueous alkaline solution at 60-80 ◦C. Uniform and compact Fe3O4 films were fabricated on nitric acid treated carbon fibers. A correlation between magnetic and absorption properties of specimens was made. It was found that the deposition time, and the sequences of the coating process have a significant effect on the reflection loss characteristics of the MCCFs. On the other hand, the temperature of the coating process affects strongly the composition of the thin film. MCCFs prepared at 80 ◦C possesses a much higher loss factor than the one prepared at 60 ◦C. The morphology, phases in the coating layer, magnetic properties and absorption behaviors of the MCCFs were examined using FESEM, XRD, permagraph, vector
Gene therapy shows promise for individualized cancer therapies. Compared to conventional treatments, this approach offers a variety of advantages, including a multiplicity of gene targets that define tumor clinical behavior, high specificity, low off target toxicity, and suppression of drug resistance. However, the lack of efficient delivery strategies for gene-specific agents and preclinical models for evaluation of the efficacy of gene-based therapeutics has impeded its clinical application. Nanoparticles provide a flexible platform for fabricating novel delivery vehicles capable of transporting gene-specific therapeutic agents to target tumors. Among the various types of nano-carriers for cancer therapy, superparamagnetic iron oxide nanoparticles have emerged as a leading candidate as they have a host of advantages compared to other formulations. The iron oxide core facilitates the condensation of other molecules to it that confer biological properties that promote stability during transport ...
Abstract: Iron oxide nanoparticles (IONPs) are promising nanomaterials for biomedical applications. However, their inflammatory potential has not been fully established. Here, we used a lepirudin anti-coagulated human whole blood model to evaluate the potential of 10 nm IONPs to activate the complement system and induce cytokine production. Reactive oxygen species and cell death were also assessed. The IONPs activated complement, as measured by C3a, C5a and sC5b-9, and induced the production of pro-inflammatory cytokines in a particle-dose dependent manner, with the strongest response at 10 µg/mL IONPs. Complement inhibitors at C3 (compstatin analog Cp40) and C5 (eculizumab) levels completely inhibited complement activation and secretion of inflammatory mediators induced by the IONPs. Additionally, blockade of complement receptors C3aR and C5aR1 significantly reduced the levels of various cytokines, indicating that the particle-induced secretion of inflammatory mediators is mainly C5a and C3a ...