Purified Native Maackia Amurensis Lectin I Protein, Biotinylated from Creative Biomart. Native Maackia Amurensis Lectin I Protein, Biotinylated can be used for research.

Leukoagglutinin and hemagglutinin (MAL I+II) are seed lectins from Maackia amurensis, and are used as glycoanalytical tools to probe biological targets for ?2-3-linked sialic acids. These lectins have a molecular weight of 130,000 and an isoelectric point of pH 4.7. MAL I+II can be used to detect glycans containing ?2-

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We demonstrate that the signaling potential of LIGHT is affected by two polymorphisms at positions E214K and S32L. The 214 position in LIGHT directly influenced the binding avidity to a neutralizing human Ab and the LTβR, indicating this residue is located in or near the receptor-binding region. This result provides an important consideration for the use of inhibitors of LIGHT, such as decoy receptors or Abs, in analyzing patient responses in clinical trials. The 32L variant located in the intracellular domain enhanced the avidity of binding to LTβR, lowered the avidity of binding to DcR3, and decreased the membrane expression of LIGHT. Although the precise mechanism of how LIGHT32L variant affects binding avidity to LTβR and DcR3 remains to be defined, it is likely an indirect effect, perhaps related to the stability of LIGHT trimer. Increased bioavailability of LIGHT due to lower avidity for DcR3 and enhanced binding avidity of LIGHT for LTβR could combine to increase signaling activity ...

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The genus Maackia is composed of 8 to 10 species all of which come from East Asia. Two of which are found in our collection. This genus is closely related to the genus Cladrastis and was once placed in there, but it differs in several important morphological characters and in its DNA. The genus is named after the Russian naturalist Richard Maack who died in 1886. We have 8 examples of Maqackia fauriei in the Arboretum. Three are from seed collected in the wild on Mt. Halla during the Nordic Arboretum Committees expedition to Korea in 1976. The rest are from seed collected from this original material. Second generation seedlings have been sent to a number of other places including: Norwegian National Arboretum, Swedens Agricultural University, Alnarp, Arboretum Trompenborg in Holland; Århus Kommune (probably the Botanic Garden, Århus). Maackia fauriei was made know to western science by Abbé Faurie in 1907, and was first introduced into European cultivation in 1917 by E. H. Wlson. It is not ...

TY - JOUR. T1 - Systemic fate of the adipocyte-derived factor adiponectin. AU - Halberg, Nils. AU - Schraw, Todd D.. AU - Wang, Zhao V.. AU - Kim, Ja Young. AU - Yi, James. AU - Hamilton, Mark P.. AU - Luby-Phelps, Kate. AU - Scherer, Philipp E.. PY - 2009/9. Y1 - 2009/9. N2 - OBJECTIVE - The adipocyte-derived secretory protein adiponectin has been widely studied and shown to have potent insulin-sensitizing, antiapoptotic, and anti-inflammatory properties. While its biosynthesis is well understood, its fate, once in circulation, is less well established. RESEARCH DESIGN AND METHODS - Here, we examine the half-life of adiponectin in circulation by tracking fluorescently labeled recombinant adiponectin in the circulation, following it to its final destination in the hepatocyte. RESULTS - Despite its abundant presence in plasma, adiponectin is cleared rapidly with a half-life of ∼75 min. A more bioactive version carrying a mutation at cysteine 39 is cleared within minutes. Even though ...

This study assessed the presence of sialic acid α-2,3 and α-2,6 linked glycan receptors in seven avian species. The respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, golden pheasant, ostrich, and mallard were tested by means of lectin histochemistry, using the lectins Maackia amurensis agglutinin II and Sambucus nigra agglutinin, which show affinity for α-2,3 and α-2,6 receptors, respectively. Additionally, the pattern of virus attachment (PVA) was evaluated with virus histochemistry, using an avian-origin H4N5 virus and a human-origin seasonal H1N1 virus. There was a great variation of receptor distribution among the tissues and avian species studied. Both α-2,3 and α-2,6 receptors were present in the respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, and golden pheasant. In ostriches, the expression of the receptor was basically restricted to α-2,3 in both the respiratory and intestinal tracts and in mallards

Abstract: : Purpose: The Guinea pig conjunctival epithelium includes a cell that shares a striking morphological resemblance to the M cells (membranous cells) found over lymphoid follicles in other mucosa. The presence of M cells in the conjunctiva would be significant since intestinal M cells are known to initiate the mucosal immune response by sampling luminal antigens and translocating them to underlying lymphocytes and antigen-presenting cells. Since intestinal M cells are also the site of entry for opportunistic pathogens such as viruses or bacteria, conjunctival M cells would also represent a previously unrecognized site of pathogen entry. We hypothesized that if the conjunctiva contains M cells, it should be possible to selectively label their apical surface glycoconjugates with lectins as has been done with M cells in other mucosal locations. Methods: We screened a panel of lectins on aldehyde-fixed nodules and identified Maakia amurensis leukoagglutinin (MAL-I) as a potential ...

This page is Related Information of Characterization of Glycan Binding Site of O-Linked Glycopeptides Using MALDI-7090 High-Resolution MALDI-TOF MS.

Use the hyperlinks on the top to navigate each of these above mentioned pathways. Currently, each arrow in a pathway is linked to a Glycoenzyme Family in the Carbohydrate Active enZYmes (CAZY) database , developed by Bernard Henrissat and Henrik Clausen . The CAZY families will be modified into lists of glycoenzymes with hyperlinks to their Consortium molecule pages analogous to the Glycan Binding Proteins Molecule Pages . Key for Glycosylation Pathways symbols ...

A new method for high throughput screening has been developed based upon the inclusion of the target receptor, chaperon protein, nuclear receptors and mitochond...

Protocol to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray.

Protocol to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform.

Determination of the binding specificity of glycan‐binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious

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Herein describes a study testing the feasibility of enhanced specificity targeting tumor cells using heterobivalent ligands. Previous work has demonstrated that two functionally unrelated GPCRs, i.e., MC4R and CCK2R, or MC4R and the δ-opioid receptor (δ-OR), can be noncovalently cross-linked by their corresponding heterobivalent ligands with high avidity (12, 17, 19). Multivalency can increase binding avidity, but not necessarily increase targeting specificity (32, 33). By following the previous in vitro findings, the current work studied the parameters including linker length and flexibility, surface receptor expression level per cell, ratio of receptor expression level, receptor-ligand affinity, and ligand concentration on which this targeting specificity depends.. The linker, Pego-[PG]3-Pego, a combination of rigid and flexible linker, demonstrated dynamic mobility over time and temperature ranges and spanned distances of 30∼70 Å as predicted by computer models (Fig. 1 B-D). As evidenced ...

FFAR2 receptor enables specific dietary constituents like dietary fiber and helps reduce food intake and protect against obesity.

It is hardly necessary to define the concept of receptors to readers of this series, but it should be borne in mind that in several instances receptors are undefined entities, whose molecular details

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SAα2,6 and SAα2,3 linked sialic acid molecules on epithelial cell membrane served as receptors for influenza virus, which are specifically recognized by human and avian influenza viruses, respectively. The distribution of these two species of sialic acids in human respiratory tract from different anatomical sites and different age groups was investigated. The results showed that SAα2,3Gal species was prevalent in respiratory bronchiole and lung alveolar epithelium, but was infrequent in trachea, bronchus and bronchiole. On the contrary, the SAα2,6Gal species was more common in the trachea and bronchus and to a lesser degree in the alveolar epithelium. When compared the expression levels of SAα2,6Gal and α2,3Gal in the respiratory tract among different age groups, no significant difference was found. In the ex vivo H5N1 virus infection study, alveolus epithelium were found to be more susceptible to avian influenza than trachea and bronchus epithelial cells. These results suggest that the ...

Introduction: The aim of this study was to compare fucose and sialic acid residue expression on fibronectin and alpha(1)-acid glycoprotein in the. seminal plasma PF-6463922 solubility dmso of men suspected of infertility and suffering from leukocytospermia.. Subjects and methods: Seminal ejaculates were collected from 27 leukocytospermic and 18 healthy, normozoospermic men. The relative degree of fucosylation and sialylation of fibronectin and alpha(1)-acid glycoprotein was estimated by ELISA using fucose and sialic acid specific lectins from Aleuria aurantia, Lotus tetragonolobus, and Ulex europaeus as well as Maackia amurensis and Sambucus nigra, respectively.. Results: Leukocytospermic seminal fibronectin, in comparison with fibronectin of normal fertile group, showed lower relative reactivity with AAL, LTA and UEA, and higher reactivity with MAA and SNA, while the AGP of the leukocytospermic group was less reactive with AAL, and the relative reactivity. buy 5-Fluoracil with LTA and MAA was ...

In the influenza virus, the two relevant antigens are the surface proteins, hemagglutinin and neuraminidase.[4] The hemagglutinin is responsible for binding and entry into host epithelial cells while the neuraminidase is involved in the process of new virions budding out of host cells.[5] Sites recognized on the hemagglutinin and neuraminidase proteins by host immune systems are under constant selective pressure. Antigenic drift allows for evasion of these host immune systems by small mutations in the hemagglutinin and neuraminidase genes that make the protein unrecognizable to pre-existing host immunity.[6] Antigenic drift is this continuous process of genetic and antigenic change among flu strains.[7]. In human populations, immune (vaccinated) individuals exert selective pressure for single point mutations in the hemagglutinin gene that increase receptor binding avidity, while naive individuals exert selective pressure for single point mutations that decrease receptor binding avidity.[6] These ...

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Takydromus amurensis, the Amur grass lizard, is a species of lizard in the family Lacertidae. It is found in Russia, China, Korea, and Japan. (Source: Wikipedia, Takydromus amurensis, https://wikipedia.org/wiki/takydromus amurensis, CC BY-SA 3.0 . Photo: (c) Kim, Hyun-tae, some rights reserved (CC BY-NC))

We sequenced and characterized the complete mitogenome (KX964606) of the Amur hedgehog Erinaceus amurensis to provide more data for comparative mitogenomics of the genus Erinaceus (Erinaceidae). The mitogenome of E. amurensis is a circular molecule 16,941 bp long, consisting of a control region and a conserved set of 37 genes containing 13 protein-coding genes, 22 tRNA genes, and two rRNA genes (12S rRNA and 16S rRNA). The mitogenome of E. amurensis is AT-biased, with a nucleotide composition of 33.9% A, 21.1% C, 32.6% T, and 12.4% G. The mitogenomes of E. amurensis and the closely related hedgehog species E. europaeus, excluding the control region (66.7%), share over 90% sequence similarity. According to the inter-generic relationship based on six mitogenomes described from five genera of Erinaceidae, the subfamilies Erinaceinae and Galericinae are strongly supported as monophyletic groups, with each genus well placed within its own subfamily. Within the subfamily Erinaceinae, E. amurensis is a sister

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.

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A method of purifying a recombinant protein from a solution, such as tissue culture fluid, containing gylcoproteins. The affinity of lectins for specific glycoproteins is assessed and used to select a

A novel label-free sensitive detection method by employing a novel sensitive charge sensor is provided. Dissociation constant information is provided by a simple measurement of the dissociation of the target molecule form the targets receptor. The later process is affected by a novel system and its configuration as described herein. Basic objectives are to provide a drug discovery and characterization system that is an improvement over the current state of the art, low cost, highly sensitive, accurate, fast and easy to use. This invention involves both a physical system and a methodology.

Using an adaptive study design, the PET study was carried out in parallel with the single ascending dose (SAD) study to relate occupancy at the A2A receptor in the brain with drug dose and plasma concentration. For each drug dose, A2A receptor occupancy was calculated for 2 plasma drug levels.. The data generated clearly demonstrated the relationship between plasma concentration and receptor occupancy in the CNS showing that the drug crosses the blood brain barrier and interacts with the target receptor. Using this data, Vernalis was able to take the drug into further development. Importantly, the quantitative data generated from imaging was used to determine the dose to be used in patient studies for efficacy, thus reducing the number of doses to be tested, saving considerable time and money.. This study clearly demonstrates the value imaging can add to aid decisions about drug development. In this way, imaging holds the key to reducing time and costs of drug development overall. ...

P-glycoprotein (P-gp) overexpression is the most frequently observed cause of multidrug resistance in neoplastic cells. In our experiments, P-gp was expressed in L1210 mice leukemia cells (S cells) by selection with vincristine (R cells) or transfection with the gene encoding human P-gp (T cells). Remodeling of cell surface sugars is associated with P-gp expression in L1210 cells as a secondary cellular response. In this study, we monitored the alteration of cell surface saccharides by Sambucus nigra agglutinin (SNA), wheat germ agglutinin (WGA) and Maackia amurensis agglutinin (MAA). Sialic acid is predominantly linked to the surface of S, R and T cells via α-2,6 branched sugars that tightly bind SNA. The presence of sialic acid linked to the cell surface via α-2,3 branched sugars was negligible, and the binding of MAA (recognizing this branch) was much less pronounced than SNA. WGA induced greater cell death than SNA, which was bound to the cell surface and agglutinated all three L1210 cell-variants

Attachment of human noroviruses to histo blood group antigens (HBGAs) is essential for infection, but how this binding event promotes the infection of host cells is unknown. Here, we employ protein NMR experiments supported by mass spectrometry and crystallography to study HBGA binding to the P-domain of a prevalent virus strain (GII.4). We report a highly selective transformation of asparagine 373, located in an antigenic loop adjoining the HBGA binding site, into an iso-aspartate residue. This spontaneous post-translational modification (PTM) proceeds with an estimated half-life of a few days at physiological temperatures, independent of the presence of HBGAs but dramatically affecting HBGA recognition. Sequence conservation and the surface-exposed position of this PTM suggest an important role in infection and immune recognition for many norovirus strains.. ...

Objective To observe the endocrine cells in the digestive tract of Rana amurensis in and out of hibernation. Methods We used the avidin-biotin-peroxidase complex (ABC) immunohistochemical method and the anti5-HT,anti-SS,anti-PP and anti-SP serums. Results 5-HT,SS and Glu cells were detected in the digestive tract of Rana Amurensis,but GAS , PP and SP cells were not. 5-HT cells distributed widely,observed throughout the digestive tract of Rana Amurensis from esophagus to rectum. The distributive density of 5-HT cells was the highest in pylorus and duodenum in hibernation,but in the pylorus during non-hibernating time.SS cells distributed widely too, observed in the whole digestive tract except the rectum. The highest density of the SS cells was both in the pylorus during the two periods. Glu cells were detected only in gastric cardia, gastric body, jejunum and ileum in hibernation,but also in rectum during the non-hibernating period.The density of Glu distribution peaks in the gastric body.Conclusion The

Chemiluminescent acridinium dimethylphenyl ester labels are used in automated immunoassays for clinical diagnostics. Light emission from these labels is triggered by alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). The surfactant plays a critical role in th

Beta-hexosaminidase; Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides; Belongs to the glycosyl hydrolase 3 family. NagZ ...

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The conductance and switching behaviour of acene series derivative `Acridinium based organic molecular single electron transistor have been assessed in the vic

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This study enabled an empirical assessment of a model carcinogen borne in soil and its potency on human receptors. SOS-lux biosensors had numerous practical advantages over the traditional assays including procedural simplicity, ease of measurement and in vivo analysis without cell disruption. A key advantage of the application of biosensors is their ability to be deployed to genuine samples. This means that while chromatographic analysis requires extraction, purification, clean-up and then analysis, the biosensor can be used in crude and turbid solutions. This could be exclusively for a carcinogenic sensor or for a wider suite of sensors for specific analytes and generic toxicity. Samples could be exposed to a suite of sensors simultaneously allowing a real time response that could prove valuable for the protection of target receptors at a timescale commensurate with intervention. The data presented confirm that the sensors could be fabricated to permit compact analysis and the use of disposable

Definition of sialoglycoproteins in the Definitions.net dictionary. Meaning of sialoglycoproteins. What does sialoglycoproteins mean? Information and translations of sialoglycoproteins in the most comprehensive dictionary definitions resource on the web.

I recently heard a radio show where two callers claimed that their blood types had changed. One caller claimed that he was born O-, but recent tests said he was A+. Another caller claimed to change from AB- to AB+. Both callers claim that they had multiple tests before and after the change.. What I noticed is that both cases claim a conversion from Rh- to Rh+, or from type O to some other type. These could potentially be explained by a person carrying a gene for Rh factor or some other blood type, but failing to express the protein. Then the gene starts expressing, changing their blood type.. Of course both stories could be false, intentionally or otherwise. This is purely anecdotal evidence, and one caller claimed that her blood type change was due to extraterrestrial interference. I couldnt find any better documented cases of blood type changes after a quick search on the internet, so I thought Id ask here.. Are there any documented cases of a change in blood type?. ...

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Feline calicivirus (FCV) is a major causative agent of respiratory disease in cats. It is also one of the few cultivatable members of the family Caliciviridae. It has recently been reported that FCV binding is in part due to interaction with junction adhesion molecule-A. This report describes the characterization of additional receptor components for FCV. Chemical treatment of cells with sodium periodate showed that FCV recognized carbohydrate moieties on the surface of permissive cells. Enzymic treatment with Vibrio cholerae neuraminidase demonstrated that sialic acid was a major determinant of virus binding. Further characterization using linkage-specific lectins from Maackia amurensis and Sambucus nigra revealed that FCV recognized sialic acid with an α2,6 linkage. Using various proteases and metabolic inhibitors, it was shown that α2,6-linked sialic acid recognized by FCV is present on an N-linked glycoprotein.

Buy Plants and Seeds | Rare and Unusual | Passiflora | Aristolochia | Asclepias | Bananas Dianthus amurensis Siberian Blues | Amur River Pink | 10 Seeds - Here I am offering seeds from Dianthus amurensis Siberian Blues, also known as Amur Pink, and Amur River Pink. This one likes to live in a spot where it can get full sun. This perennial flower grows to about a foot tall, and is a huge hit with the birds,

TY - JOUR. T1 - Exosome-SIRPα, a CD47 blockade increases cancer cell phagocytosis. AU - Koh, Eunee. AU - Lee, Eun Jung. AU - Nam, Gi Hoon. AU - Hong, Yeonsun. AU - Cho, Eunji. AU - Yang, Yoosoo. AU - Kim, In-San. PY - 2017/3/1. Y1 - 2017/3/1. N2 - CD47, a dont eat me signal, is over-expressed on the surface of most tumors that interacts with signal regulatory protein α (SIRPα) on phagocytic cells. By engaging SIRPα, CD47 limits the ability of macrophages to engulf tumor cells, which acts as a major phagocytic barrier. In this study, we developed an exosome-based immune checkpoint blockade that antagonizes the interaction between CD47 and SIRPα. These exosomes harboring SIRPα variants (SIRPα-exosomes) were sufficient to induce remarkably augmented tumor phagocytosis, lead to prime effective anti-tumor T cell response. Given that clustering of native CD47 provides a high binding avidity to ligate dimerized SIRPα on macrophage, nature-derived exosomes could be appreciable platform to ...

In the few years since the beginning of glycomics in 21th century, many achievements have been made in the study of glycomics, especially in the discovery of sugar genes and the elucidation of the new structure of sugar chain. Although glycomics research has made remarkable achievements in these fields, there are many unknown intermediate links between sugar genes and sugar chains, sugar chains and glycan binding proteins, and there is a general lack of detailed research on the relationship between structure and function.. These fields are not only related to the progress of glycomics itself, but also directly related to the progress of proteome planning, so it has become a major bottleneck in the development of life science.. The advancement of analytical techniques has always been a direct driving force in the development of glycomics. It is the rapid development and wide application of modern instrument analysis technology that lead to the overall prosperity of glycomics research, and inject ...

Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.

This is a Phase 1, dose escalation study of EC0489 administered by intravenous bolus (IV) during weeks 1 and 3 of a 4-week cycle in PART A and weekly on a 4-week cycle in PART B.. Both Parts are open to patients with refractory or metastatic cancer who have exhausted standard therapeutic options. EC0489 is a drug that is specifically designed to enter cells via a folate vitamin receptor. Experimental evidence shows that the target receptor is over-expressed in many human cancers. There are no previous human studies of EC0489; however, lab research (research in test tubes or animals) using EC0489 has shown activity against tumors in animals. This activity in animal models suggests that EC0489 may be useful as chemotherapy against human cancers. The primary objective of this study is to determine the safety and maximum tolerated dose of EC0489 given by intravenous bolus. The efficacy of treatment will also be measured. ...