TY - JOUR. T1 - Nicotinic acetylcholine receptor. T2 - evidence for a functionally distinct receptor on human lymphocytes.. AU - Richman, David P. AU - Arnason, B. G.. PY - 1979/9. Y1 - 1979/9. N2 - The presence of three distinct cholinergic receptors on human lymphocytes was suggested by the effects of carbamoylcholine on lymphocyte proliferation in vitro. The cells responded to both 0.1 nM and 1 microM carbamoylcholine by increased proliferation which was blocked by the muscarinic antagonist atropine. This effect occurred in both mitogen-stimulated cells (maximum effect at 24 hr) and nonstimulated cells (maximum effect at 72 hr). In contrast, 1--10 nM carbamoylcholine produced diminished in vitro proliferation, an effect which was blocked by the nicotinic antagonists alpha-bungarotoxin and d-tubocurarine.. AB - The presence of three distinct cholinergic receptors on human lymphocytes was suggested by the effects of carbamoylcholine on lymphocyte proliferation in vitro. The cells responded to ...
Are neutrophil/lymphocyte ratio and platelet/lymphocyte ratio reliable parameters as prognostic indicators in malignant mesothelioma? Seda Tural Onur,1 Sinem Nedime Sokucu,1 Levent Dalar,2 Sinem Iliaz,1 Kaan Kara,1 Songül Buyukkale,3 Sedat Altin1 1Department of Chest Diseases, Yedikule Chest Diseases and Thoracic Surgery Training and Research Hospital, 2Department of Pulmonary Medicine, Faculty of Medicine, Istanbul Bilim University, 3Department of Thoracic Surgery, Yedikule Chest Diseases and Thoracic Surgery Training and Research Hospital, Istanbul, Turkey Background: Malignant mesothelioma (MM) is an aggressive asbestos-related pleural tumor. The incidence is increasing with intensive use of asbestos in developing countries. We need an easily accessible, inexpensive, and reliable method for determining the low survival time prognosis of this tumor. The aim of our study was to investigate the viability of neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) as prognostic
Início Cytogenetic Studies: Premature Chromossome Condensation (PCC) in Peripheral Blood Lymphocytes Cultures and Skin Warts Lesions Cultures Induced by Papillomavirus Spontaneus Infection in ...
Helicobacter pylori (HP) can cause many diseases and malignant conditions. In the stomach, HP causes mucosal injury and inflammation. We determined the association of the platelet/lymphocyte ratio (PLR) and neutrophil/lymphocyte ratio (NLR) with HP-positive and HP-negative gastritis severity. We retrospectively reviewed 234 patients who had undergone upper gastrointestinal endoscopy for the investigation of dyspepsia and reflux symptoms. The patients were divided into three groups according to the results of the histopathologic evaluation: HP-negative gastritis group, HP-positive gastritis group and control group (neither HP positivity nor gastritis). We routinely collected laboratory data, including complete blood count, from all the patients. The mean neutrophil count was higher and the mean lymphocyte count was lower in the HP-positive gastritis group than in the other groups. The mean platelet count was increased in all groups, but the lowest increase was seen in the HP-positive gastritis ...
Reactive lymphocytes or variant lymphocytes are cytotoxic (CD8+) lymphocytes that become large as a result of antigen stimulation. Typically, they can be more than 30 µm in diameter with varying size and shape. The nucleus of a reactive lymphocyte can be round, elliptic, indented, cleft, or folded. The cytoplasm is often abundant and can be basophilic. Vacuoles and/or azurophilic granules are also sometimes present. Most often, the cytoplasm is gray, pale blue, or deep blue in colour. The distinctive cell associated with EBV or CMV is known as a Downey cell, after Hal Downey, who contributed to the characterization of it in 1923. Reactive lymphocytes are usually associated with viral illnesses, but they can also be present as a result of drug reactions (such as phenytoin), immunizations, radiation, and hormonal causes (such as stress and Addisons disease), as well as some autoimmune disorders (such as rheumatoid arthritis). Some pathogen-related causes include: Epstein-Barr virus ...
TY - JOUR. T1 - Investigation of K+ channel expression in human peripheral lymphocytes of healthy donors by means of flow cytometry. AU - Krjukova, J.. AU - Osna, N.. AU - Pilmane, M.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Evaluation of different types of K+ channel expression was performed in resting and PHA (phytohemagglutinine)-activated human peripheral lymphocytes (HPL) of healthy donors by means of flow cytometry. In resting peripheral lymphocytes, the application of kaliotoxin (a selective blocker for voltage-dependent K+ (K(V)) channels), K(V) resulted in pronounced depolarization of lymphocyte membrane potential, with further promotion in the presence of thapsigargin (compound discharging Ca(i) from endoplasmic reticulum). In activated HPL, the expression of various types of K+ channels was estimated utilizing cell-cycle analysis data. In contrast to the resting cells, kaliotoxin-induced depolarization of membrane potential in PHA-activated lymphocytes of the G0/G1 phase was not enhanced ...
Genotoxic effects of tacrolimus on human lymphocyte cells.: We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which is u
Masuda, T; Miyama, M; Kuribayashi, K; Yodoi, J; Takabayashi, A; and Kyoizumi, S, Immunological properties of fc receptor on lymphocytes. V. Suppressive regulation of humoral immune response by fc receptor bearing b lymphocytes. (1978). Subject Strain Bibliography 1978. 3145 ...
TY - JOUR. T1 - Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines. AU - Bolt, Alicia M.. AU - Byrd, Randi M.. AU - Klimecki, Walter T.. PY - 2010/5/1. Y1 - 2010/5/1. N2 - Arsenic is a widespread environmental toxicant with a diverse array of molecular targets and associated diseases, making the identification of the critical mechanisms and pathways of arsenic-induced cytotoxicity a challenge. In a variety of experimental models, over a range of arsenic exposure levels, apoptosis is a commonly identified arsenic-induced cytotoxic pathway. Human lymphoblastoid cell lines (LCL) have been used as a model system in arsenic toxicology for many years, but the exact mechanism of arsenic-induced cytotoxicity in LCL is still unknown. We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to ...
Background: There is a direct relation between C-reactive protein and leukocyte count as indicators of inflammation. The neutrophil lymphocyte ratio (NLR) is the balance between both cells and is considered a marker of low-grade inflammation and an indicator of high risk of cardiovascular events. An elevated NLR is related with type 2 diabetes (T2D), however, even when in type 1 diabetes (T1D) there is an underlying inflammatory process, an elevation of circulating neutrophils isn t observed. NLR is associated with hyperglycemia in adult patients with long-duration T2D. However, reports in pediatric populations with recent-onset type 1 (T1D) and T2D are scarce.. Objective and hypotheses: To evaluate the association between NLR and the degree of glycemic control in pediatric patients with recent-onset T1D and T2D vs healthy controls.. Method: Design: Analytical cross-sectional. Subjects 8 16 years of age were included, with T1D or T2D with diagnosis ≤ 3 months, who, with their guardians, signed ...
The Predictive Value of Total Neutrophil Count and Neutrophil/ Lymphocyte Ratio in Predicting In-hospital Mortality and Complications after STEMI ...
Aim Systemic and local therapies can be used to treat painful bone metastases. It has been shown that certain pharmaceuticals such as Re-186 (rhenium-186) are effective in the treatment of pains caused by bone metastasis and a correlation between bone metastases and T cells has also been shown. The aim of this study was to investigate the genotoxic effect of Re-186-1,1-hydroxyethylidenediphosphonate (Re-186-HEDP) on the cultured peripheral blood lymphocytes using an micronucleus (MN)-fluorescence in-situ hybridization assay. ...
Rivnay, B; Globerson, A; and Shinitzky, M, Viscosity of lymphocyte plasma membrane in aging mice and its possible relation to serum cholesterol. (1979). Subject Strain Bibliography 1979. 2694 ...
The neutrophil-to-lymphocyte ratio (NLR) and the platelet-to-lymphocyte ratio (PLR) are associated with poor prognoses in patients with gastric cancer; however, few studies have focused on the dynamic changes in these ratios during the treatment of patients with gastric cancer. Here, we assessed the clinical utility of changes in these ratios as prognostic indicators in patients with stage II or III gastric cancer who received adjuvant chemotherapy. We retrospectively reviewed 100 patients who received S-1 adjuvant chemotherapy at ≥70% of the relative dose intensity, and their NLRs and PLRs were evaluated at different times: prior to gastrectomy and upon commencement and termination of adjuvant chemotherapy. To assure the clinical utility of the changes in NLR and PLR as prognostic indicators, other clinical factors were assessed as well. Disease recurred in 35 patients as follows: lymph node metastasis (17 patients, 17.0%), peritoneal metastasis (12 patients, 12.0%), and hematogenous metastasis (6
The coronavirus disease 2019 (COVID-19) has become a pandemic. Rapidly distinguishing COVID-19 from other respiratory infections is a challenge for first-line health care providers. This retrospective study was conducted at the Taipei Medical University Hospital, Taiwan. Patients who visited the outdoor epidemic prevention screening station for respiratory infection from February 19 to April 30, 2020, were evaluated for blood biomarkers to distinguish COVID-19 from other respiratory infections. Monocyte distribution width (MDW) ≥ 20 (odds ratio [OR]: 8.39, p = 0.0110, area under curve [AUC]: 0.703) and neutrophil-to-lymphocyte ratio (NLR) | 3.2 (OR: 4.23, p = 0.0494, AUC: 0.673) could independently distinguish COVID-19 from common upper respiratory tract infections (URIs). Combining MDW ≥ 20 and NLR | 3.2 was more efficient in identifying COVID-19 (AUC: 0.840). Moreover, MDW ≥ 20 and NLR | 5 effectively identified influenza infection (AUC: 0.7055). Thus, MDW and NLR can distinguish COVID-19 from
Background: In this study, we explored the relationship between                  neutrophil-to-lymphocyte ratio (NLR) and grade 3 or higher treatment related small bowel toxicity and treatment outcome of patients with rectal cancer undergoing capecitabine and concurrent intensity modulated radiotherapy (IMRT). Materials and Methods: From the year of 2012 to 2013, 117 rectal cancer ...
BACKGROUND: The preoperative neutrophil-to-lymphocyte ratio (NLR) and the platelet-to-lymphocyte ratio (PLR) are associated with poor prognosis of gastric cancer. We aimed to determine whether the combination of NLR and PLR (NLR-PLR) could better predict survival of patients after curative resection for stage I-II gastric cancer.. METHODS: We collected data from the medical records of patients with stage I-II gastric cancer undergoing curative resection between December 2000 and November 2012 at the Sun Yat-sen Cancer Center. The preoperative NLR-PLR was calculated as follows: patients with both elevated NLR (≥2.1) and PLR (≥120) were given a score of 2, and patients with only one or neither were given a score of 1 or 0, respectively.. RESULTS: Kaplan-Meier analysis and log-rank tests revealed significant differences in overall survival (OS) among patients with NLR-PLR scores of 0, 1 and 2 (P , 0.001). Multivariate analysis showed that OS was independently associated with the NLR-PLR score ...
Neutrophil-To-Lymphocyte Ratio (NLR) Platelet-To-Lymphocyte Ratio (PLR), and Outcomes With Nivolumab in Pretreated Non-Small Cell Lung Cancer (NSCLC): A Large Retrospective Multicenter Study
The prognostic significance of neutrophil-to-lymphocyte ratio at different time points in patients with hepatocellular carcinoma receiving liver resection
Postoperative neutrophil-to-lymphocyte ratio change is a potential marker for the early detection of recurrence in urothelial carcinoma
Palmitoleic acid (PA) is a n-7 monounsaturated fatty acid (MUFA) secreted by adipose tissue and related to decreased insulin resistance in peripheral tissues. Evidences have been shown that PA also decreased proinflammatory cytokine expression in cultured macrophages. Although studies have shown that other fatty acids (FAs) modulate several lymphocyte functions, the specific effect of PA on these cells is unknown. The aim of the present study was to evaluate the possible influence of PA on activation and differentiation of human lymphocytes in comparison to oleic acid (OA). Human lymphocytes were isolated from peripheral blood of health men and cultured in the presence of growing concentrations of PA or OA (5 to 200 μM), for 24 h. After that, cells were collected and cytotoxicity evaluated by flow cytometry. Then, we analyzed proliferative capacity in lymphocytes treated with non toxic concentrations of PA and OA (25 and 50 μM, respectively), in the presence or absence of concanavalin A (ConA). The
In medicine neutrophil to lymphocyte ratio (NLR) is used as a marker of subclinical inflammation. It is calculated by dividing the number of neutrophils by number of lymphocytes, usually from peripheral blood sample, but sometimes also from cells that infiltrate tissue, such as tumor. Higher NLR is independent predictor of mortality in patients undergoing angiography or cardiac revascularization. Increased NLR is associated with poor prognosis of various cancers, such as esophageal cancer or pancreatic cancer. Wang X (Mar 2014). Neutrophil to lymphocyte ratio in relation to risk of all-cause mortality and cardiovascular events among patients undergoing angiography or cardiac revascularization : A meta-analysis of observational studies. Atherosclerosis. 234 (1): 206-13. doi:10.1016/j.atherosclerosis.2014.03.003. PMID 24681815. Wang J (Jan 2014). The clinical significance of tumor-infiltrating neutrophils and neutrophil-to-CD8+lymphocyte ratio in patients with resectable esophageal squamous ...
The effect of specific antigen on the development of physical interactions between lymph node lymphocytes (LNL) obtained from animals which had been immunized to that antigen and macrophages was examined. We found that the presence of antigen, either limited to the macrophage () or free in the medium, profoundly increased the degree of ) or free in the medium, profoundly increased the degree of Mphi-LNL interaction observed. This enhanced interaction was dependent on the coincidence in the cultures of Mphi bearing antigen and LNL from animals specifically immunized to that antigen. Although antigen-independent interactions developed equally well between syngeneic and allogeneic combinations of lymphocytes and macrophages, antigen mediated interactions required that macrophages and lymphocytes be syngeneic. Prolongation of antigen-mediated Mphi-LNL interactions resulted in the induction of LNL DNA synthesis, initially involving those lymphocytes physically associated with antigen-bearing Mphi. ...
A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000- …
Saferali A, Grundberg E, Berlivet S, Beauchemin H, Morcos L, Polychronakos C, Pastinen T, Graham J, McNeney B, Naumova AK. Cell culture-induced aberrant methylation of the imprinted IG DMR in human lymphoblastoid cell lines. Epigenetics. 2010 Jan 01; 5(1):50-60 ...
Novelty: This study describes the kinetic changes in lymphocyte subsets and cytokine profiles and identifies neutrophil-to-CD8+ T cell ratios and neutrophil-to-lymphocyte ratios as prognostic factors for disease severity. Standing in the field: It has been previously shown that lymphopenia and pro-inflammatory cytokine storm are involved in COVID-19 disease. This is the first study to explore the changes in lymphocyte count and pro-inflammatory cytokine number over the course of COVID-19 disease. Appropriate statistics: Yes Viral model used: SARS-CoV-2 Translatability: This study validates the use of neutrophil-to-CD8+ T cell ratio and neutrophil-to-lymphocyte ratio as prognostic markers for COVID-19 disease severity. This may be used to allow clinicians to identify infected patients at higher risk of severe COVID-19. Main limitations: Small sample size. The cohort size (40 patients) is too small to draw broad conclusions from, particularly as the severe group is made up of only 13 patients, 3 ...
The lymphocyte-high endothelial venule (HEV) cell interaction is an essential element of the immune system, as it controls lymphocyte recirculation between blood and lymphoid organs in the body. This interaction involves an 85-95-kD class of lymphocyte surface glycoprotein(s), CD44. A subset of lymphocyte CD44 molecules is modified by covalent linkage to chondroitin sulfate (Jalkanen, S., M. Jalkanen, R. Bargatze, M. Tammi, and E. C. Butcher. 1988. J. Immunol. 141:1615-1623). In this work, we show that removal of chondroitin sulfate by chondroitinase treatment of lymphocytes or incubation of HEV with chondroitin sulfate does not significantly inhibit lymphocyte binding to HEV, suggesting that chondroitin sulfate is not involved in endothelial cell recognition of lymphocytes. Affinity-purified CD44 antigen was, on the other hand, observed to bind native Type I collagen fibrils, laminin, and fibronectin, but not gelatin. Binding to fibronectin was studied more closely, and it was found to be ...
Different radiation-techniques in treating local staged prostate cancer differ in their dose- distribution. Physical phantom measurements indicate that for 3D, less healthy tissue is exposed to a relatively higher dose compared to SSIMRT. The purpose is to substantiate a dose distribution in lymphocytes in-vivo and to discuss the possibility of comparing it to the physical model of total body dose distribution. For each technique (3D and SSIMRT), blood was taken from 20 patients before and 10 min after their first fraction of radiotherapy. The isolated leukocytes were fixed 2 hours after radiation. DNA double-strand breaks (DSB) in lymphocytes nuclei were stained immunocytochemically using the gamma-H2AX protein. Gamma-H2AX foci inside each nucleus were counted in 300 irradiated as well as 50 non-irradiated lymphocytes per patient. In addition, lymphocytes of 5 volunteer subjects were irradiated externally at different doses and processed under same conditions as the patients lymphocytes in order to
Neutrophil to lymphocyte ratio see Neutrophil to lymphocyte ratio for subarachnoid hemorrhage. see Neutrophil to lymphocyte ratio for glioma. see Neutrophil to lymphocyte ratio for intracerebral hemorrhage. Normal Neutrophil to lymphocyte ratio values in an adult, non-geriatric, population in good health are between 0.78 and 3.53. These data may help the researcher as the clinician searching for a cut-off for the NLR, until now lacking
TY - JOUR. T1 - Low blood lymphocyte count at 30 days post transplant predicts worse acute GVHD and survival but not relapse in a large retrospective cohort. AU - Gul, Z.. AU - Van Meter, E.. AU - Abidi, M.. AU - Ditah, I.. AU - Abdul-Hussein, M.. AU - Deol, A.. AU - Ayash, L.. AU - Lum, L. G.. AU - Waller, E. K.. AU - Ratanatharathorn, V.. AU - Uberti, J.. AU - Al-Kadhimi, Z.. PY - 2015/3/1. Y1 - 2015/3/1. N2 - Multiple reports have shown that low absolute lymphocyte count at day 30 (ALC30) after allogeneic hematopoietic SCT (AHSCT) is associated with higher risk of disease relapse and worse OS. However, these reports included heterogeneous populations with different grafts and GVHD prophylaxis. Therefore, we retrospectively evaluated the association of ALC30 with transplant outcomes in a cohort of 381 consecutive patients who underwent AHSCT between 2005 and 2010 and received T-replete PBSC grafts and Tacrolimus/Mycophenolate combination as GVHD prophylaxis. Median follow-up was 57 months. ...
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Mitogens and antigens have been the traditional ligands for activating lymphocytes in vitro for the elaboration of lymphokines. Recently, histamine, by interaction with histamine-type 2 receptors on T lymphocytes, has been found to induce the production of one lymphokine, histamine-induced suppressor factor (HSF), that inhibits lymphocyte proliferation and lymphokine production in vitro. Because the biologic effects of HSF appear to be confined to alterations in lymphocyte function, we assessed the ability of soluble products of histamine-stimulated human blood mononuclear cells to affect another lymphocyte function, motility. Utilizing a modified Boyden chamber assay to assess lymphocyte migration, we identified chemoattractant activity for human blood and rat splenic T lymphocytes in histamine-induced mononuclear cell supernatants. No neutrophil or monocyte chemoattractant activity was present. Sephadex G-100 gel filtration of histamine-induced supernatants showed the lymphotactic activity ...
Surface receptor expression on human lymphocytes and neutrophils. Neutrophils and lymphocytes were isolated from the whole blood of different human donors and i
TY - JOUR. T1 - Illusory DNA breaks in quiescent lymphocytes?. AU - Boerrigter, Michael E.T.I.. AU - Mullaart, Erik. AU - van der Schans, Govert P.. AU - Vijg, Jan. PY - 1990/5. Y1 - 1990/5. UR - http://www.scopus.com/inward/record.url?scp=0025241827&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0025241827&partnerID=8YFLogxK. U2 - 10.1016/0014-4827(90)90269-G. DO - 10.1016/0014-4827(90)90269-G. M3 - Article. C2 - 2328767. AN - SCOPUS:0025241827. VL - 188. JO - Experimental Cell Research. JF - Experimental Cell Research. SN - 0014-4827. IS - 1. ER - ...
In their search for antigens, lymphocytes continuously shuttle among blood vessels, lymph vessels, and lymphatic tissues. Chemokines mediate entry of lymphocytes into lymphatic tissues, and sphingosine 1-phosphate (S1P) promotes localization of lymphocytes to the vasculature. Both signals are sensed through G protein-coupled receptors (GPCRs). Most GPCRs undergo ligand-dependent homologous receptor desensitization, a process that decreases their signaling output after previous exposure to high ligand concentration. Such desensitization can explain why lymphocytes do not take an intermediate position between two signals but rather oscillate between them. The desensitization of S1P receptor 1 (S1PR1) is mediated by GPCR kinase 2 (GRK2). Deletion of GRK2 in lymphocytes compromises desensitization by high vascular S1P concentrations, thereby reducing responsiveness to the chemokine signal and trapping the cells in the vascular compartment. The desensitization kinetics of S1PR1 allows lymphocytes to ...
Objectives: To investigate the intercourse between the platelet/lymphocyte (P/L) and neutrophil/lymphocyte ratio (N/L), and vitamin D (Vit-D) levels in low bone mineral density (BMD) of women. ...
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BACKGROUND Neutrophil to lymphocyte ratio (NLR) was reported to be an independent easy-to-measure marker for many diseases, while the distribution of NLR in healthy population was not formally studied. So we investigated NLR in healthy people in this study. METHODS Data include NLR, systolic pressure, diastolic pressure, and body mass index (BMI), which were collected from 3,262 healthy population. Correlations of NLR with variables were analyzed with SPSS16.0. RESULTS Results demonstrated that NLR was positively associated with age (P | 0.001). The eldest age group possessed the highest NLR and the youngest age group had the lowest NLR. NLR was also slightly positively associated with systolic pressure, diastolic pressure, and BMI (P | 0.001). CONCLUSION Older people possessed relatively high NLR in healthy population.
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Introduction: Acute appendicitis (AA) may interfere many diseases and appendectomy is the most performed intra-abdominal operation in the world. Despi..
My son had a blood smear test done and the nurse told me it came back showing some abnormal lymphocytes in it. She said that is normal, but I dont believe her. Does anyone know? The doctor wont be...
Lymphocyte and plasma cell disorders can be classified into three major groups (Table 78-1). The first group, listed under primary disorders, is composed of lymphocyte disorders caused by intrinsic defects in lymphoid cells that result in functional abnormalities of marrow-derived (B) lymphocytes, thymic-derived (T) lymphocytes, combined T and B (impaired humoral and cellular immunity), or natural killer (NK) cells. These disorders primarily result from inborn errors in lymphocyte metabolism (Chaps. 73 to 77 and 80) and/or receptor-ligand expression (Chaps. 17 and 80). The second group, listed under acquired disorders, consists of disorders caused by factors extrinsic to lymphocytes resulting in immune dysfunction. These conditions most commonly result from infection with viruses, or other cellular pathogens (Chaps. 79, 81, and 82), but they also may be caused by bacteria, drugs or systemic disease of nonlymphoid cells. The third group of diseases is composed of preneoplastic and neoplastic ...
Two small lymphocyte subpopulations from human peripheral blood were produced by rosette sedimentation procedures, and tested for in vitro functions by stimulation with mitogens, PPD and allogeneic lymphocytes, and for effector capacities in ADCC. The non-T, non-Fc subpopulation had previously been found to be a SmIg+ fraction which did not aquire new surface markers during unstimulated culture, did not respond to mitogens, PPD or allogeneic cells but were potent MLC stimulators. In addition, they were poor ADCC effector cells. The non-T, non-B (Null) cells, which showed varying surface marker profiles from donor to donor, were able to respond to mitogens and PPD, but were weak MLC responders. Potent ADCC effector capacity was, however, found to be exhibited by this subpopulation. ...
FTY720 (Fingolimod) reduces multiple sclerosis disease activity by inducing lymphopenia and inhibiting lymphocyte re-entry from lymph nodes. Peripheral lymphocyte reconstitution following drug discontinuation has been considered relatively rapid (2-4weeks), based on short-term studies. Authors investigated the kinetics of lymphocyte reconstitution in MS patients in open label extension phases of FTY720 clinical trials who discontinued therapy after prolonged use (,1-5years), and examined histological features of a mediastinal lymph node obtained from a lymphopenic FTY720 patient. Although three patients showed reconstitution of peripheral lymphocytes within the predicted timeline, two patients continued to be lymphopenic 9 and 34months after therapy cessation. Lymph nodes from the latter patient showed preserved architecture. Notwithstanding preserved lymph node integrity, time for lymphocyte reconstitution after prolonged FTY720 therapy can be significantly greater than predicted by ...
NFAT2 null mutant mice die in utero of cardiac failure, precluding analysis of the role of NFAT2 in lymphocyte responses. Only the NFAT2-/-/Rag-1-/- chimeric mice model gave insight into the role of NFAT2 transcription factor in T lymphocyte development, activation and differentiation. As reports are mainly focused on the role of NFAT2 in CD4+ T lymphocytes activation and differentiation, we decided to investigate NFAT2s impact on CD8+ T lymphocytes responses. We report that NFAT2 is phosphorylated and inactive in the cytoplasm of naive CD8+ T cells, and upon TCR stimulation is dephosphorylated and translocated into the nucleus. To study the role of NFAT2 in CD8+ T responses we employed NFAT2fl/flCD4-Cre mice with NFAT2 deletion specifically in T cells. Interestingly, the absence of NFAT2 in T cells resulted in increased percentage of nonconventional innate-like CD8+ T cells. These cells were CD122+, rapid producer of IFN-γ and had characteristics of conventional memory CD8+ T cells. We also observed
Accurate monitoring of host immunity is hampered by the flaws of conventional tests. The relationship between lymphocyte number and function is unknown. The function of lymphocytes was analyzed based on IFN-γ secretion assay. Lymphocyte number and function was investigated in individuals under various states. The number of CD4+ and CD8+ T cells was gradually decreased, whereas the function of them was gradually increased with increasing age. A significantly negative correlation existed between the number and function of both CD4+ and CD8+ T cells. Differently, both the number and function of NK cells are maintained at a high level after birth. Staying up all night was found to impair the function of CD4+, CD8+ T cells, or NK cells. Lymphocyte number and function were both decreased in patients with immunosuppressive conditions or opportunistic infections, while the opposite phenomenon was observed in patients with some autoimmune
The cross-linking of human peripheral lymphocyte surface Ig results in an early association of cyclic adenosine 3:5-monophosphate (cAMP) and the cell surface Ig patches. Examination of the subsequent stages of cap formation reveals the continued relationship of cAMP and the clustered surface Ig. In addition, the generalized influx of calcium produced by the ionophore A-23187 disrupts human lymphocyte caps. During the process of cap dissolution cAMP is still associated with surface Ig. Therefore, it is hypothesized that the localized concentration of cyclic nucleotide and calcium ion regulates the movement of cell surface constituents by coordinating the function of the cells contractile and structural elements. ...
Chemoattractants control lymphocyte recruitment from the blood, contributing to the systemic organization of the immune system. The G protein-linked receptor GPR15 mediates lymphocyte homing to the large intestines and skin. Here we show that the 9 kDa CC-motif containing cationic polypeptide AP57/colon-derived SUSD2 binding factor (CSBF), encoded by C10orf99 in the human and 2610528A11Rik in the mouse, functions as a chemokine ligand for GPR15 (GPR15L). GPR15L binds GPR15 and attracts GPR15-expressing T cells including lymphocytes in colon draining lymph nodes and Vγ3+ thymic precursors of dermal epithelial T cells. Patterns of GPR15L expression by epithelial cells in adult mice and humans suggest a homeostatic role for the chemokine in lymphocyte localization to the large intestines, as well as a role in homing to the epidermis during wound healing or inflammation. GPR15L is also significantly expressed in squamous mucosa of the oral cavity and esophagus with still poorly defined regulation.
TY - JOUR. T1 - In vitro activation of HIV RNA expression in peripheral blood lymphocytes as a marker to predict the stability of non-progressive status in long-term survivors. AU - Garbuglia, Anna R.. AU - Salvi, Roberto. AU - Di Caro, Antonino. AU - Cappiello, Giuseppina. AU - Montella, Francesco. AU - Di Sora, Fiorella. AU - Recchia, Olga. AU - Lauria, Filippo. AU - Benedetto, Arrigo. PY - 1996. Y1 - 1996. N2 - Objectives: We investigated a selected group of 11 non-progressor, HIV-infected individuals 20 months prior to this study and found that they all had undetectable levels of viral RNA expression in their peripheral blood lymphocytes (PBL). Phorbol 12-myristate 13-acetate (PMA) and phytohaemagglutinin (PHA) stimulation of PBL produced easily detectable amounts of HIV RNA in only two out of five of these patients. Here we report the results of the virological and clinical follow-up of nine non-progressors from this group. We verified the stability of their non-progressive status and ...
TY - JOUR. T1 - The combination of neutrophil-to-lymphocyte ratio and serum carbohydrate antigen 19-9 level as a prognostic indicator in patients with recurrent pancreatic cancer. AU - Sakamoto, Teruhisa. AU - Saito, Hiroaki. AU - Uchinaka, Ei. AU - Morimoto, Masaki. AU - Amisaki, Masataka. AU - Tokuyasu, Naruo. AU - Honjo, Soichiro. AU - Ashida, Keigo. AU - Fujiwara, Yoshiyuki. PY - 2018/9. Y1 - 2018/9. N2 - Background/Aim: We retrospectively investigated the relationship between prognosis and combined neutrophil-to-lymphocyte ratio (NLR) and serum carbohydrate antigen 19-9 (CA19-9) levels in patients with recurrent pancreatic cancer. Patients and Methods: We enrolled 66 patients whose pancreatic cancer recurred. Results: Based on ROC analysis results, the patients were divided into NLRHigh (NLR ≥1.69) or NLRLow (NLR ,1.69), and into CA19-9High (CA19-9 ≥107.95 U/ml) or CA19-9Low (CA19-9 ,107.95 U/ml). When the patients were grouped by combined NLR and CA19-9, their 2-year survival rates ...
MACHADO, G.; CALADO, R. T.; GARCIA, Aglair Bergamo; FALCÃO, Roberto Passetto. P-glicoprotein function is influrnced by age in normal human peripheral blood lymphocytes and bone marrow stem cells. The Hematology Journal[S.l: s.n.], 2002 ...
The effects of cytochalasin B on functional and physical macrophage-lymphocyte interaction have been examined. Cytochalasin B, an inhibitor of a variety of membrane activities blocks antigen-dependent binding of immune lymphocytes to macrophages and antigen-triggered lymphocytes proliferation if added at the initiation of culture. Cytochalasin B becomes progressively less inhibitory if addition is delayed by increasing intervals from the onset of culture. Under these conditions neither antigen handling by macrophages nor the proliferative response of lymphocytes to PHA is inhibited by cytochalasin B. These data are interpreted to suggest that cytochalasin B inhibits antigen-specific macrophage-lymphocyte interaction either by inhibition of an initial antigen-independent phase of macrophage-lymphocyte interaction or by interfering with a lymphocyte membrane event necessary for the interaction of the antigen-specific lymphocyte receptor with the macrophage-bound antigenic signal.. ...
TY - JOUR. T1 - Effect of maturation and age on lymphocyte proliferation induced by A23187 through an interleukin-independent pathway. AU - Wu, W.. AU - Pahlavani, M.. AU - Richardson, A.. AU - Cheung, H. T.. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 1985. Y1 - 1985. N2 - Lymphocyte proliferation induced by lectins declines drastically with age. It has been suggested that the reduction of interleukin production by lymphocytes from old individuals is responsible for the decline in proliferation. In this study, lymphocyte proliferation was stimulated by the calcium ionophore, A23187. A23187 induced the proliferation of spleen lymphocytes from rats through an interleukin-independent pathway; depletion of spleen lymphocytes of macrophages, addition of exogenous interleukin-2 (IL 2), and addition of anti-IL 2 monoclonal antibodies had no effect on the proliferation stimulated by A23187. Spleen lymphocytes from male Fischer F344 male rats of 5, 13, 22, and 30 months of ...
Red cell distribution width (RDW) and the neutrophil/lymphocyte ratio (NLR) are predictors of cardiovascular risk that have been shown to correlate with impaired reperfusion and increased morbidity and mortality in patients with an ST-segment elevati
The Comparison of Apache Ii Scores with Neutrophil Lymphocyte Ratio and Red Cell Distribution Width for the Prediction of Prognosis of Patients with Acute Pancreatitis, Murat Orak,
Background: Neutrophil-to-lymphocyte ratio and platelets-to-lymphocyte ratio may be host factors associated with prognosis. We sought to determine whether neutrophil-to-lymphocyte and platelets-to-lymphocyte ratio were associated with overall survival among patients undergoing surgery for intrahepatic cholangiocarcinoma. Methods: Patients who underwent resection for intrahepatic cholangiocarcinoma between 1990 and 2015 were identified from 12 major centers. Clinicopathologic factors and overall survival were compared among patients stratified by neutrophil-to-lymphocyte ratio and platelets-to-lymphocyte ratio. Risk factors identified on multivariable analysis were included in a prognostic model and the discrimination was assessed using Harrells concordance index (C index). Results: A total of 991 patients were identified. Median neutrophil-to-lymphocyte ratio and platelets-to-lymphocyte ratio were 2.7 (interquartile range [IQR]: 2.0-4.0) and 109.6 (IQR: 72.4-158.8), respectively. Preoperative ...
Early and accurate risk assessment is an important clinical demand in patients with infective endocarditis (IE). The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are independent predictors of prognosis in many infectious and cardiovascular diseases. Very limited studies have been conducted to evaluate the prognostic role of these markers in IE. We analyzed clinical, laboratory, and echocardiographic data and outcomes throughout the whole period of hospitalization for a total of 142 consecutive patients with definitive IE. The overall in-hospital mortality was 21%. Major complications defined as central nervous system embolization, fulminant sepsis, acute heart failure, acute renal failure, and major artery embolization occurred in 38 (27%), 34 (24%), 32 (22.5%), 40 (28%), and 90 (63.4%) patients, respectively. The NLR, total leucocyte count (TLC), neutrophil percentage, creatinine, and C-reactive protein (CRP) level obtained upon admission were significantly higher in the
Preoperative neutrophil-to-lymphocyte ratio is an independent prognostic marker in patients with laryngeal squamous cell carcinoma. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Objectives: Peptic ulcer perforation (PUP) accounts for 5% of all abdominal emergencies and is recognized as a gastrointestinal emergency requiring rapid and efficient clinical evaluation and treatment. The mortality rate ranges from 10% to 40% among patients with perforation. In the present retrospective study, we examined the potential utility of the neutrophil-to-lymphocyte ratio (NLR) in early diagnosis of PUP; we asked whether this ratio allowed PUP and peptic ulcer disease to be distinguished. ...
In vitro studies have indicated that the antifolates pyrimethamine [4, 6] and cycloguanil (the active metabolite of proguanil) suppress the proliferation of stimulated human lymphocytes; proguanil has no effect [2]. During the early growth phase of the cells, 14C-thymidine (14C-TdR) incorporation is increased by pyrimethamine and cycloguanil, reflecting blockage of endogenous TdR synthesis [3]. Proguanil (Paludrine) is increasingly being used for malaria prophylaxis. It is considered the most innocuous of the antimalarials currently employed. Since nothing is known about the effect of oral proguanil on human lymphocytes, the present study was undertaken. Little information is available about the serum levels of proguanil and cycloguanil following ingestion of prophylactic doses [8]. Therefore, the serum concentrations of proguanil and cycloguanil were estimated, to allow comparison with previous in vitro studies [2 ...
A one-step separation procedure is described for both depleting and obtaining in pure form Fc receptor (FcRL), C3 receptor (CRL) and surface immunoglobulin bearing (IgL) lymphocytes from rat lymphoid populations. The method is a modification of the Bӧyum (1968) technique for separating lymphocytes from whole blood by sedimentation on Ficoll/Isopaque, and is based on the fact that when a lymphocyte forms a rosette with sensitized erythrocytes it will sediment with the red cells rather than float with the non-rosetting lymphocytes. The technique is , 99.5% efficient at depleting thoracic duct lymphocytes (TDL) of FcRL, CRL and IgL and these subpopulations can be recovered 93-98% pure. The total recovery of lymphocytes applied is usually , 90% and the separated lymphocytes are , 95% viable. This technique allowed the cellular distribution of Fc receptors, C3 receptors and surface Ig to be determined. It was found that (a) Almost all CRL carry surface Ig, although a very small sub-population of ...
cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA contains a PF00059 domain.. cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA contains a PF00084 domain.. cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA contains a PF00084 domain.. cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA contains a PF00008 domain.. cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA is proteolytically cut by matrix metallopeptidase-3 (M10.005) cleavage... cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA is proteolytically cut by matrix metallopeptidase-1 (M10.001) cleavage... cDNA, FLJ93066, Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL),mRNA is proteolytically cut by ADAM17 peptidase (M12.217) cleavage. KLDK-SFSM.. ...
Background/aims: COPD is among the significant health complications in entire world; studies have shown that there is systemic inflammation with pulmonary inflammation in case of COPD. To detect the systemic inflammatory response, neutrophil lymphocyte ratio (NLR) in peripheral blood which is important biomarker. NLR hasnt been studied in patients having chronic obstructive pulmonary disease (COPD). Current study was targeted to determine the importance of NLR as inflammatory marker in patients with COPD.Methods: The neutrophil and lymphocyte count in the peripheral blood was found out from blood count (CBC) reports. The NLR was determined by dividing neutrophil count from lymphocyte count. COPD patients were diagnosed with Spirometry. Then the NLR was compared in patients having stable COPD (n = 50), and healthy controls (n = 50.Results: The neutrophil count was considerably greater in COPD patients matched with the healthy individuals (p | 0.001) whereas the lymphocyte count was suggestively lower in
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TY - JOUR. T1 - Induction and disappearance of DNA strand breaks in human peripheral blood lymphocytes and fibroblasts treated with methyl methanesulfonate. AU - Boerrigter, Michael E.T.I.. AU - Mullaart, Erik. AU - Vijg, Jan. N1 - Funding Information: This work was supported by grants from Senetek p.l.c., the Sandoz Foundation for Gerontological Research, and the Dutch Ministry of Welfare, Health and Cultural Affairs. We thank Drs. F. Berends and G. Douglas for critically reading the manuscript and Mr. M. Boer-mans and Mr. R. van Boven for preparing the figures. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 1991/1. Y1 - 1991/1. N2 - The induction and disappearance of DNA single-strand breaks (SSB) in human peripheral blood lymphocytes (PBL) and fibroblasts exposed to methyl methanesulfonate (MMS) were investigated by using the alkaline filter elution assay. In the two cell types, identical amounts of SSB were induced during a 45-min treatment with a given dose of MMS. In ...
Dose-response curves obtained 1 and 3 days after exposure to total-body X irradiation indicate that the mononuclear cells in the circulating blood and in the peritoneal cavity of LAF sub1 mice may be arranged in the following order of decreasing sensitivity: circulating lymphocytes, small peritoneal lymphocytes, medium peritoneal lymphocytes, peritoneal macrophages. However, on the 3rd day postirradiation, the curve of the small peritoneal lymphocytes closely approached that of the circulating lymphocytes. It is suggested that the greater sensitivity to irradiation of small than of medium peritoneal lymphocytes is not due to environmental factors but to as yet unidentified differences in the cells. On the other hand, the greater loss of circulating lymphocytes than of small peritoneal lymphocytes 1 day after X ray exposure may merely reflect more efficient removal of damaged cells from the circulation than from the peritoneal cavity. (Author)(*LYMPHOCYTES
There is increasing evidence that CD8 lymphocytes may represent targets for infection by human immunodeficiency virus type 1 (HIV-1) in vivo whose destruction may contribute to the loss of immune function underlying AIDS. HIV-1 may infect thymic precursor cells destined to become CD4 and CD8 lymphocytes and contribute to the numerical decline in both subsets on disease progression. There is also evidence for the induction of CD4 expression and susceptibility to infection by HIV-1 of CD8 lymphocytes activated in vitro. To investigate the relationship between CD8 activation and infection by HIV-1 in vivo, activated subsets of CD8 lymphocytes in peripheral blood mononuclear cells (PBMCs) of HIV-seropositive individuals were investigated for CD4 expression and HIV infection. Activated CD8 lymphocytes were identified by expression of CD69, CD71, and the human leukocyte antigen (HLA) class II, the beta-chain of CD8, and the RO isoform of CD45. CD4(+) and CD4(-) CD8 lymphocytes, CD4 lymphocytes, other T cells,
Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is an uncommon subtype of Hodgkin lymphoma (HL). This disorder is differentiated from all other forms of HL, which are commonly referred to as classical Hodgkin lymphomas (cHLs), by characterist
Multiple sclerosis (MS) is a chronic autoimmune disease caused by immune cell infiltration and damage to the central nervous system (CNS). Therapies that target lymphocyte trafficking in MS patients have shown promise, but current approaches can have major side-effects, caused in part by lack of specificity for homing to the CNS. Therefore, more specific tools to inhibit lymphocyte recruitment to the CNS without affecting systemic trafficking of immune cells are needed for the treatment of MS. Using cutting-edge in vivo multi-photon microscopy to image lymphocyte infiltration into the CNS, this project will test a novel approach aimed at more specifically preventing lymphocyte extravasation into the CNS. Our goal is to determine how the lymphocyte cytoskeleton mediates the steps required for T cell infiltration through the blood-brain barrier and into the CNS during autoimmunity. We hypothesize that lymphocyte extravasation through the specialized CNS vasculature walls relies on transcellular ...
Using RNA hybridization techniques, we examined the expression of proto-oncogenes associated with lymphocyte activation in vitro in patients with systemic lupus erythematosus and other autoimmune diseases. T and B lymphocytes from these patients were found to have significantly increased expression of c-myc, c-myb, and c-raf RNA when compared with those of normal individuals. Among the mononuclear cell subpopulations, B lymphocytes expressed higher levels of RNA for these proto-oncogenes compared with the T lymphocytes. Since prompt expression of these and other proto-oncogenes occurs in fibroblasts and lymphocytes following mitogenic stimulation, we propose that the present findings reflect the pathologically activated state of various lymphocytic subpopulations which is observed in systemic lupus erythematosus and in other autoimmune diseases. Endogenous and exogenous factors which lead to the expression of autoimmunity might share the induction of proto-oncogene expression as a common ...
Mammalian stem cells differentiate into several kinds of blood cell within the bone marrow.[3] This process is called haematopoiesis. All lymphocytes originate, during this process, from a common lymphoid progenitor before differentiating into their distinct lymphocyte types. The differentiation of lymphocytes follows various pathways in a hierarchical fashion as well as in a more plastic fashion. The formation of lymphocytes is known as lymphopoiesis. B cells mature into B lymphocytes in the bursa equivalent, which in humans is the GALT, which is thought to be located in the Peyers patches of the intestine,[4] while T cells migrate to and mature in a distinct organ, called the thymus. Following maturation, the lymphocytes enter the circulation and peripheral lymphoid organs (e.g. the spleen and lymph nodes) where they survey for invading pathogens and/or tumor cells. The lymphocytes involved in adaptive immunity (i.e. B and T cells) differentiate further after exposure to an antigen; they form ...
La Plata, Argentina. ABSTRACT The effect of co-culturing varying concentrations of pig and human red blood cells (RBCs) on the baseline frequency of sister chromatid exchanges (SCEs) and cell-cycle progression in pig plasma (PLCs) and whole blood leukocyte cultures (WBCs) was studied. No variation in SCE frequency was observed between pig control WBC and PLC. Addition of pig and human RBCs to pig PLCs did not modify the baseline frequency of SCEs. On the other hand, cell proliferation was slower in PLCs than in WBCs. The addition of pig or human RBCs to PLCs accelerated the cell-cycle progression of pig lymphocytes. When RBCs were added to PLCs the concentration and time sequence of RBC incorporation affected the cell-cycle progression of swine lymphocytes. When doses of pig or human RBCs equivalent to those present in WBCs were added immediately after PLC stimulation, the cell-cycle kinetics were similar to those of WBCs. Shorter co-incubation periods or a reduction in the dose of RBCs made ...
immune Uncategorized Keratin 7 antibody, purchase Flumazenil 0 Comment Purpose To review the role of the neutrophil:lymphocyte ratio (NLR) and monocyte:lymphocyte ratio (MLR) in discriminating between different patient groups hospitalized for fever due to contamination and those without contamination. the other bacterial infection groups. Conclusions NLR is usually a more useful diagnostic tool to identify patients with septicemia than other more commonly used diagnostic blood assessments. NLR and MLR may be useful in the diagnosis of bacterial infection among patients hospitalized for fever. One hundred and fifty patients with a diagnosis of bacterial infection supported by microbiology, serology, or radiology of which 69 experienced pneumonia, 30 urinary tract contamination, and 27 experienced septicemia. Fourteen patients with a diagnosis of viral contamination supported by microbiology, serology or radiology. Of these, purchase Flumazenil nine suffered from infectious mononucleosis. ...
TY - JOUR. T1 - Antigen-pulsed neutrophils bearing Ia antigens can induce T lymphocyte proliferative response to the syngeneic or semisyngeneic antigen-primed T lymphocytes. AU - Okuda, K.. AU - Tani, K.. AU - Ishigatsubo, Y.. AU - Yokota, S.. AU - David, C. S.. PY - 1980. Y1 - 1980. N2 - Antigen-pulsed neutrophils from mouse peritoneal cavities displayed a remarkable level of lymphocyte proliferative activities to antigen-primed T lymphocytes. Genetic mapping studies demonstrated that compatibility at the I-A, as well as I-E/C, subregions of the major histocompatibility complex (MHC) was essential for effective presentation of the lysozyme antigen. These antigen-presenting activities were remarkably inhibited by anti-Ia sera. Inhibition tests revealed that neutrophil immune-associated (Ia) antigens seem to be essential for antigen presentation during the initial 8 hr. Elimination studies of antigen-pulsed neutrophils with alloantisera plus complement revealed these antigen-presenting ...
A cobalamin-dependent N5-methyltetra-hydrofolate-homocysteine methyltransferase (methyl-transferase) was demonstrated in unfractioned extracts of human normal and leukemia leukocytes. Activity was substantially reduced in the absence of an added cobalamin derivative. Presumably, this residual activity reflects the endogeneous level of holoenzyme. Enzyme activity was notably higher in lymphoid cells than in myeloid cells. Thus, mean specific activities (+/-SD) were: chronic lymphocytic leukemia lymphocytes, 2.15+/-1.16; normal lymphocytes, 0.91+/-0.59; normal mature granulocytes, 0.15+/-0.10; chronic myelocytic leukemia granulocytes, barely detectable activity. Properties of leukocytes enzymes resembled those of methyltransferases previously studied in bacteria and other animal cells. Granulocytes and chronic myelocytic leukemia cells contain a factor or factors that inhibits Escherichia coli enzyme. The data suggest that the prominence of this cobalamin-dependent enzyme in lymphocytes and other ...
Purpose: We aimed to establish an inflammatory prognostic index (IPI) in early and advanced non-small cell lung cancer (NSCLC) patients based on hematologic and biochemical parameters and to analyze its predictive value for NSCLC survival. Materials and Methods: A retrospective review of 685 patients with early and advanced NSCLC diagnosed between 2009 and 2014 was conducted with collection of clinical, and laboratory data. The IPI was calculated as C-reactive protein × NLR (neutrophil/ lymphocyte ratio)/serum albumin. Univariate and multivariate analyses were performed to assess the prognostic value of relevant factors. Results: The optimal cut-off value of IPI for overall survival (OS) stratification was determined to be 15. Totals of 334 (48.8%) and 351 (51.2%) patients were assigned to high and low IPI groups, respectively. Compared with low IPI, high IPI was associated with older age, greater tumor size, high lymph node involvement, distant metastases, advanced stage and poor performance status.
By studying a mutation that causes specific defects in lymphocyte cytotoxicity, we have delineated a critical role for STAT4 in regulating Munc13-4 expression upon cytotoxic lymphocyte differentiation and identified a novel, evolutionarily conserved promoter within intron 1 of UNC13D. The intronic c.118-308C,T mutation causative of FHL3 abolished an ELF1-binding site that is necessary for the recruitment of STAT4 and BRG1 as well as the acquisition of histone marks permissive for transcription. TCR stimulation of naive CD8+ T cells led to robust induction of Munc13-4, STAT4, and BRG1, and knockdown of STAT4 abrogated induction of Munc13-4 expression. Our data show that the UNC13D intronic sequence can act both as an alternative promoter and as a general enhancer, promoting Munc13-4 expression upon cytotoxic lymphocyte differentiation.. A surprising finding from our analysis of lymphocyte subsets was that Munc13-4 expression levels are selectively up-regulated upon cytotoxic lymphocyte ...
Patients with rheumatoid arthritis (RA), patients with positive tuberculin (PPD) skin-tests and controls were studied. The lymphocytes from these groups were cultured in serum-free medium to obtain cell-free supernatants. These lymphocyte cultures were pre-incubated with the appropriate antigen or reconstituted after removal of the cells. Supernatants from RA lymphocytes stimulated in vitro by IgG induced an inhibition of the leucocyte migration, as well as the supernatants from tuberculin-sensitized lymphocytes. However, supernatants from non-RA lymphocytes or tuberculin-unsensitized lymphocytes did not show such an inhibition. These MIF-like supernatants have been studied by Sephadex G-100 gel filtration. A MIF activity has been found for PPD and IgG supernatants between the chymotrypsinogen (MW 23,000) and the lysozyme (MW 17,000). This seems to agree with the classical region where MIF can be usually isolated.
Young women being underweight is a public health problem in Japan. The aim of this study was to investigate this problem by measuring lymphocyte count as an indicator of nutritional status. The subjects were 114 women aged 20-39 who participated in an annual health checkup for residents in a city in Aichi, Japan. Data from a questionnaire, physical examination, and blood tests were analyzed in relation to women who were severely underweight [body mass index (BMI) ≤ 17.5 kg/m2], slightly underweight (17.5 | BMI | 18.5 kg/m2), of normal weight (18.5 ≤ BMI | 25 kg/m2), and obese (BMI ≥ 25 kg/m2). Lymphocyte count tended to be lower with a decrease in BMI. The prevalence of low lymphocyte count of |1,500/mm3 increased in underweight women. In women who had restricted food intake for weight loss, leukocyte count, and total serum protein, and lymphocyte count were lower. A multivariate logistic regression analysis showed the association of low lymphocyte count to being severely underweight [odds ratio
Accessible surface proteins of thymus-derived lymphocytes (T-cells) of normal CBA mice and bone-marrow-derived lymphocytes (B-cells) of congenitally athymic nu/nu mice were analysed. The surfaces of lymphocytes were radioiodinated by using the enzyme lactoperoxidase (EC 1.11.1.7), then solubilized either in acid-urea or in the non-ionic detergent Nonidet P-40. These lysates were then precipitated with antisera specific to either immunoglobulin or the θ-alloantigen in order to assess the presence of these surface markers. Comparable amounts of radioactivity in proteins specifically precipitable as immunoglobulin were obtained from T-lymphocytes and B-lymphocytes when the cells were disrupted by acid-urea. This immunoglobulin had mol. wt. approx. 180000 and was composed of light chains and μ-type heavy chains. When radioiodinated lymphocytes were solubilized with Nonidet P-40, 3-4% of radioiodinated high-molecular-weight protein of B-cells consisted of immunoglobulin, a result similar to that ...
Background: The present study investigated the potential roles of plasma lymphocyte DNA damage, the urotensin-2 receptor (UTS2R), and oxidative changes in patients with varying degrees of migraine-related disability who were in the ictal phase and presented to our emergency department. ...
The pathological importance of tumor necrosis factor (TNF)-alpha in rheumatoid arthritis (RA) is now widely accepted. Ex vivo data from synovial cell cultures suggest that direct cell contact between activated T-cells and macrophages may be an important driver of macrophage TNF-alpha production in the RA joint. However, the ligand/receptor pairs driving this cell contact signal remain obscure. One reason for this is that plasma membrane (PM) proteins are resistant to systematic analysis using traditional proteomic approaches. In this chapter we present a method for the enrichment and resolution of PM proteins from murine T-cell hybridomas as a prelude to identification by tandem mass spectrometry. We used cell surface biotinylation, differential centrifugation and subsequent streptavidin affinity capture, followed by solution phase iso-electric focussing and tandem mass spectrometry to identify 75 PM proteins and make semiquantitative comparisons of resting and activated cells. The method is applicable
The T lymphocyte or T cell is mature in thymus. T Lymphocyte is also called cytotoxic (poisonous cell) or killer T lymphocyte. The T cell kill directly all the cell having specific antigen on it is surface which has been recognized by T cells. The helper T lymphocyte controls the strength and quality of all immune responds. Mature lymphocyte cell flow constantly in blood toward thymus gland and back into the blood to monitor the body from invader substances continuously ...
The high NLR group had a low complete response (CR) rate compared to low NLR group (51.6% vs. 83.5%; p,0.001). The 5-year PFS and OS rates were 27.4% and 30% in the high NLR group and 61.1% and 63.7% in the low NLR group, respectively (both p,0.001). Multivariate analyses confirmed that NLR is one of the independent risk factors for failure to achieve CR and for worse PFS and OS. The nomogram showed superior discrimination ability for predicting 5-year PFS and OS rates compared with NCCN-IPI (c index 0.78 vs. 0.75 and 0.79 vs. 0.75, respectively).. ...
Peripheral blood lymphocytes from 9 monoclonal gammopathies of undetermined significance (MGUS) and 27 multiple myelomas (MM) were studied with a panel of monoclonal antibodies (MoAb) that recognize B and T lymphocytes and plasma cells. No difference in the percentage of B lymphocytes, identified by B1 and B4 MoAb, was observed in MGUS and MM patients versus normal controls. However, high percentages of circulating lymphocytes expressing plasma cell-associated antigens were detected in MM (HAN-PC1+ = 29.4 +/- 20.4%; TEC-T10+ = 27.8 +/- 19.2%) whereas they were in the normal range in MGUS (HAN-PC1+ = 8.8 +/- 5.8% p = 0.006; TEC-T10+ = 5.7 +/- 4.7% p less than 0.001). Almost identical results were obtained using PCA-1 MoAb in 17 of these patients. TEC-T10+ and PCA-1+ lymphocytes were sorted and re-analyzed with phycoerythrin conjugated MoAb in 3 healthy subjects, 2 MGUS, and 4 MM patients. In normal subjects and in MGUS the majority of PCA-1+ cells belonged to the B lineage (Leu 2-, Leu3-, Leu ...
This retrospective trial evaluated the efficacy of ipilimumab [Yervoy] in patients with metastatic malignant melanoma, with a focus on a possible association
AMAÇ: Akut apandisitin tanısı zor olabilmektedir. Bu retrospektif çalışmada, nötrofil/lenfosit oranının (NLR) akut apandisit tanısındaki ve komplike apandisit ile basit apandisit ayrımındaki belirleyiciliği araştırıldı. GEREÇ VE YÖNTEM: Akut apandisit tanısı ile ameliyat edilmiş 1067 hastanın kayıtları retrospektif olarak incelendi. Histopatolojik değerlendirmeye göre hastalar akut apandisit (G1) ve normal appendiks (G2) olarak iki ana gruba ayrıldı. Akut apandisit grubundaki hastalar ise yeniden basit apandisit ve komplike (perfore ve gangrene) olarak alt iki gruba ayrıldı. BULGULAR: G1 grubunda 897, G2 grubunda 170 hasta vardı. G1ada 753, G1bde ise 144 hasta vardı. Akut apandisit tanısı konulmasında NLR için cut-off değeri 4.68 (p,0.001) olarak hesaplandı. Sensitivite %65.3, spesifite ise %54.7 idi. Komplike apandisit ayırımı için NLR cut-off değeri 5.74 olarak hesaplandı. Sensitivitesi %70.8, spesifitesi ise %48.5 idi. TARTIŞMA: Akut apandisit ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
TY - JOUR. T1 - Regulation of human mixed lymphocyte culture reactions. T2 - augmentation of proliferation and generation of cytotoxic cells. AU - Suthanthiran, Manikkam. AU - Lagman, M.. AU - Rubin, A. L.. AU - Stenzel, K. H.. AU - Novogrodsky, A.. PY - 1978/12/1. Y1 - 1978/12/1. N2 - The in vitro proliferation and generation of cytotoxic cells in the mixed lymphocyte culture reaction (MLC) are thought to mimic in vivo cellular immune effector mechanisms operative in allograft rejection. Recent studies have clarified the need for disparity at the D-locus-determined antigens between the stimulator and responder cell populations in the allogeneic MLC reactions. However, very little is known about the initial cell surface interactions of transmembrane propagation of stimulatory signals in the MLC. Since alterations in cell shape or surface architecture could conceivably influence these processes and since the microtubular apparatus is the prime candidate for a cell surface modulating structure, we ...
To test the role of intracellular tyrosines of VEC more systematically and in an EC system more relevant to inflammation, VEC mutants containing single Y to F substitutions were transiently expressed as N-terminal fusion proteins of EGFP in GPNT cells. Expression levels and overall localization of the individual VEC mutants were similar and mostly restricted to the periphery of the cell (Fig. 5A). Some cells expressed the protein at very high levels throughout the cell but these appeared non-viable and were never integrated into endothelial monolayers. In all other expressing cells, EGFP was almost exclusively found at cell-cell junctions once EC monolayers were formed (Fig. 5B). Again we observed that the introduction of Y731F-VEC significantly reduced lymphocyte migration. In addition we also observed impaired lymphocyte migration across GPNT cells that expressed Y645F-VEC or Y733F-VEC (Fig. 5C). By contrast, the introduction of wt VEC or any other Y to F mutant did not affect migration. The ...
CURI, Rui; WILLIAMS, J. F.; NEWSHOLME, Eric A. Formation of ketone bodies by resting lymphocytes. International Journal of Biochemistry, Oxford, v. 21, n. 10, p. 1133-1136, 1989. DOI: 10.1016/0020-711x(89)90055-4 ...