PML is required for p73 transcriptional and proapoptotic activity. (A) A bax promoter-driven luciferase reporter plasmid (bax-Luc) alone or in combination with

There was a positive correlation between CEA levels and CA 15-3 levels and patient prognosis. A high specificity for B-cells was confirmed by assaying for luciferase reporter gene expression from a plasmid containing an analogous combination of immunoglobulin heavy chain regulatory ...

Luciferase assays: Use a secreted luciferase reporter for promoter studies in bacteria, cultured cells, and transgenic plants or animals.

Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.

Both the luciferase gene and a human housekeeping gene ACTB (both expressed only in human cells) were present only in the injected kidney tissue, as confirmed by the absence of the both housekeeping ACTB and luciferase gene in non-injected kidneys (Figure 3D ...

Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors pMCS-Gaussia Luc Vector Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors...

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit 100-rxn kit Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay...

Gaussia Luciferase (GLuc) reporter gene offers bright bioluminescence, either as a standalone expression monitor or as a fusion partner with other protein.

The half-life of GLuc remains unclear. All GLuc-expressing vectors available for mammalian expression at NEB harbor the humanized codons for GLuc in which the secreted GLuc has activity similar to that of the native protein. Once GLuc is secreted into the culture medium, it is very stable. For example, ~20% of the GLuc activity was detectable after incubating a GLuc-containing sample at 99˚C for 15 minutes ...

TY - GEN. T1 - Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay in the human T47D breast cancer cell. AU - Legler, J.. AU - van den Brink, C.. AU - Brouwer, A.. AU - van der Saag, P.. AU - Vethaak, A.D.. AU - Murk, A.J.. AU - van der Burg, B.. PY - 2000. Y1 - 2000. M3 - Conference paper. SP - 104. EP - 108. BT - Endocrine Disrupting Compounds : Wildlife and human health risks, The Hague 1998. CY - The Hague. ER - ...

The original aim of this thesis was to utilise Vibrio harveyi luciferase as a reporter of the expression of cell division genes during the cell cycle. Several plasmids expressing luxAB genes from ftsZ promoters were constructed. To achieve maximal luciferase expression, the ribosome binding site in front of the luxA gene was improved, which led to increased expression of luciferase. The level of expression of the improved luciferase reporter from plasmids was sufficiently high to be detected in single cells, although not high enough to be used in lower copy number constructs. However, luciferase activity showed significant fluctuations that did not appear to be linked to cell cycle events. These fluctuations made the detection of any cell cycle related changes in luciferase expression impossible. Another direction of this thesis is represented by the studies on the topology of the cell shape determining RodA protein. The ampicillin resistance levels were measured in 52 fusions with the topology ...

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.. ...

p21WAF1/CIP1 plays a major role in the induction of G1 arrest following DNA damage. Although p21WAF1/CIP1 expression is regulated by the tumor suppressor p53, induction of p21WAF1/CIP1 expression through p53-independent pathways has been described in numerous cell types. In this report, we describe the mechanism by which the retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) induces p21WAF1/CIP1 in breast carcinoma cells possessing either a wild-type (MCF-7 cells) or mutated (MDA-MB-468 cells) p53. Exposure of MDA-MB-468 cells to this retinoid results in an approximately 10-fold increase in p21WAF1/CIP1 mRNA levels, whereas less than a 2-fold increase in p21WAF1/CIP1 gene transcription was observed as indicated by transient transfection experiments utilizing a p21WAF1/CIP1 promoter firefly luciferase reporter gene construct and nuclear run-off studies. We found similar results in the MCF-7 cells (Z-M. Shao et al., Oncogene, 11: 493-504, 1995). We have now found ...

To confirm the functionality of LODER-driven siRNA in vivo, we tested the ability of a LODER containing siRNA targeting the luciferase gene, siLuc LODER, to reduce luciferase expression in normal and tumor tissues constitutively expressing the luciferase gene. We therefore implanted empty or siLuc LODERs into the livers of transgenic mice expressing the luciferase gene (MUP-Luc) in liver cells (19). In vivo imaging results showed that siLuc LODERs led to a significant decrease in luciferase levels compared with empty LODERs (Fig. S5A). Next, LODERs carrying siLuc or siGFP were implanted into CT26 cell-derived s.c. synograft tumors. Three days after implantation, measurements of luciferase activity in vivo revealed that siLuc released from the LODERs inhibited luciferase expression (Fig. S5B). This decrease in luciferase activity was not correlated to nonspecific effects on tumor growth, as tumor weights were similar within both groups (Fig. S5C). Together these results show that siRNA is ...

Luciferase Reporter Gene Assay Kit - Information The Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light ...

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In this application note, we measure luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals.

Luciferase assay products include reporter assay reagents, a comprehensive range of reporter vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of reporter vectors to the cell line of your choice.

Material and Methods: miR-150 expression was quantified by qRT-PCR in two MIBC cell lines (5637 and T24). After successful miR-150 inhibition by transfection, MTS and transwell assays were used to assess the MIBCs cisplatin sensitivity and cell invasiveness, respectively. The TargetScan database and a luciferase reporter system were used to identify whether the programmed cell death 4 protein (PDCD4) is a direct target of miR-150 in MIBC cells ...

The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.

IL-8 is a direct target of miR-106a. (a) Up: potential sites in IL-8 3′UTR targeted by miR-106a. 3′UTR of IL-8 was cloned into a luciferase reporter vector.

Plasmid pGL3-NFAT luciferase from Dr. Jerry Crabtrees lab contains the insert 3x NFAT binding sequence and is published in Nature. 1992 Jun 25. 357(6380):695-7. This plasmid is available through Addgene.

Buy Luciferase recombinant protein-BAF48390 (MBS203675) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE

To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...

Four staggering, previously unreleased Luc Ferrari works c.1973-1992 are cued up for the first time, marking what would have been the late, great

The Dual Luciferase Reporter Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates. ...

Аннотация: Сells and tissues are composed from atoms of chemical elements, some of which have two kinds of stable isotopes, magnetic and nonmagnetic ones. Not long ago, magnetic isotope effects (MIEs) have been discovered in experiments with cells enriched with magnetic or nonmagnetic isotopes of magnesium. These MIEs can stem from higher efficiency of the enzymes of bioenergetics in the cells enriched with magnetic magnesium isotope. In the studies of MIEs in biological systems, it is needed to monitor the ATP concentrations as the major energy source in cells. The most sensitive and rapid method of the ATP measurements is based on the use of the firefly luciferase-luciferin system. Since luciferase is the ATP-dependent enzyme and activated by Mg-ions, it is necessary to elucidate whether this enzyme is sensitive to magnetic field of the magnesium isotopes nuclear spin. Herein we present the results of studying the effects of different isotopes of magnesium, magnetic 25Mg and ...

Tubulointerstitial fibrosis is a progressive process affecting the kidneys, causing renal failure that can be life-threatening. Thus, renal fibrosis has become a serious concern in the ageing population; however, fibrotic development cannot be diagnosed early and assessed noninvasively in both patients and experimental animal models. Here, we found that serum amyloid A3 (Saa3) expression is a potent indicator of early renal fibrosis; we also established in vivo Saa3/C/EBPβ-promoter bioluminescence imaging as a sensitive and specific tool for early detection and visualization of tubulointerstitial fibrosis. Saa3 promoter activity is specifically upregulated in parallel with tumor necrosis factor α (TNF-α) and fibrotic marker collagen I in injured kidneys. C/EBPβ, upregulated in injured kidneys and expressed in tubular epithelial cells, is essential for the increased Saa3 promoter activity in response to TNF-α, suggesting that C/EBPβ plays a crucial role in renal fibrosis development. Our ...

Lab Reagents Human IgG antibody Laboratories manufactures the single or dual luciferase assay reagents distributed by Genprice. The Single Or Dual Luciferase Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact luciferase assay. Other Single products are available in stock. Specificity: Single Category: Or Group: Dual Luciferase. Dual Luciferase information ...

BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.

Dual luciferase assay - proper controls? - posted in Cell Biology: Hey everyone, I am trying to perform a dual luciferase reporter assay in a cell line with promoters established from another cell line. cDNA and protein of the gene are present in both cell lines. Problem: In the new cell line, the luciferase activity for my promoters of interest is below pGL3, whereas TKpGL3 is as highly active as in the other cell line. Renilla activity was equal in all probes. So I am looking for appr...

Bioluminescent Reporter plasmid to express bacterial luciferase (Lux) in Mycobacteria Background. Bioluminescence, the production of light by luciferase-catalysed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localisation of luciferase-expressing cells within an animal. Applied to the study of infectious diseases, BLI permits the detection of microorganisms from within living animals thus allowing the spatiotemporal study of infection in real-time in the same host. Moreover, using luciferase as a reporter of gene expression, it is possible to establish when and where a gene function is needed, shedding light on bacterial pathogenesis. Finally, BLI constitutes an easy and rapid method to test novel antimicrobial compounds in vivo.. Three main luciferin-luciferase systems have been utilised for BLI. The first system is represented by ...

0140] The in vitro transfection efficiency of complexes prepared with modified bPEI-2 polymer and luciferase reporter gene was investigated using HepG2 and SK-OV-3 cell lines. HepG2 cells were seeded onto 24-well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. SK-OV-3 cells were seeded onto 24 well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. After 24 hours, the plating media were replaced with fresh growth media, followed by the drop-wise addition of 50 microliters of complex solution containing 2.5 micrograms luciferase plasmid DNA at various N/P ratios. Following 4 hours of incubation, free complexes were removed by replacing the medium in each well. After a further 68 hours of incubation, the cell culture medium in each well was removed and the cells rinsed once with 0.5 mL of phosphate-buffered saline (PBS, pH 7.4). For luciferase expression assay, 0.2 mL of reporter lysis buffer was added to ...

The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the course of the luciferase reaction have not been reported. Here, we show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, adenosine triphosphate (ATP), are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously ...

Data Availability StatementThe datasets used during the present study are available from the corresponding author upon reasonable request. negative control using Lipofectamine? 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). After 48 h, cells were lysed and assayed for luciferase activity using a dual luciferase reporter assay (Promega Corporation). Normalized luciferase activity was reported as luciferase activity/luciferase activity. Statistical analysis All data are presented as the mean standard deviation. SPSS 21.0 statistical software (IBM Corp., Armonk, NY, USA) was used to explore the statistical analysis. Comparisons between two groups were conducted using two-tailed Students t-test and multiple group comparisons were conducted via one-way analysis of variance with Tukeys post hoc test. P 0.05 was considered to indicate a statistically significant difference. Results miR-106b-3p is upregulated in ESCC tissues and cell lines The expression of miR-106b-3p in 50 paired ESCC ...

Alibaba.com offers 155 luminometer products. About 27% of these are testing equipment, 25% are other measuring & analysing instruments, and 6% are other optics instruments. A wide variety of luminometer options are available to you,

Title:Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to Skeletal Muscle. VOLUME: 15 ISSUE: 1. Author(s):Maria C. Subang, Rewas Fatah, Ying Wu, Drew Hannaman, Jason Rice, Claire F. Evans, Yuti Chernajovsky and David Gould. Affiliation:Bone & Joint Research Unit, Queen Mary University of London, William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UK.. Keywords:Gene therapy, luciferase, microRNA, plasmid DNA, skeletal muscle, tissue-specific promoter, transgene immunogenicity.. Abstract:Immune responses to expressed foreign transgenes continue to hamper progress of gene therapy development. Translated foreign proteins with intracellular location are generally less accessible to the immune system, nevertheless they can be presented to the immune system through both MHC Class I and Class II pathways. When the foreign protein luciferase was expressed following intramuscular delivery of plasmid DNA in outbred ...

a,b, Western blotting analysis of miR-20a target genes in response to increasing doses of miR-20a mimic (a) or antagomir (b). c,d, RT-qPCR analysis of miR-20a levels in HeLa cells transfected with miR-20a mimic (c) or antagomir (d). e,f, RT-qPCR analysis of DAPK3 mRNA levels in HeLa cells transfected with miR-20a mimic (e) or antagomir (f). g, Luciferase reporter constructs containing the miR-20a MRE from DAPK3 (native) in Renilla 5 UTR or mutated to restore base-pairing in the 5 end (seed) coupled with progressive mismatches (3MM+seed) in the 3 end. h,i, Results of the luciferase reporter assays (h) and quantification of luciferase mRNA (i). j,k, mRNA levels of luciferase reporters containing individual MREs from 4 indicated genes that function in both CDS and 3UTR (j) or those that function only in CDS (k), as shown in main Fig. 2e,f. l,m, Luciferase activities from the reporters containing individual MREs from 4 indicated genes that function only in CDS in response to co-transfected ...

Neutralizing anti-drug antibodies (NAbs) against therapeutic interferon beta (IFNβ) in people with multiple sclerosis (MS) are measured with cell-based bioassays. The aim of this study was to redevelop and validate two luciferase reporter-gene bioassays, LUC and iLite, using a cut-point approach to …

Cells transfected with 8 pmol of luc2-encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 104 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μg eGFP- or luc2-encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no ep) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 104 viable cells was measured by luminescence in triplicate ...

Supernatants from three individual wells per experimental condition were collected in the indicated time points and assayed for the IL-8 concentration using a human being IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend). et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S2? Multiple strains induce TIFA-dependent signaling in epithelial cells. (A) Control, KO#1 AGS cells (DKO) were cocultured with the indicated strains (MOI = 10), and IL-8 concentration in the supernatant were measured by ELISA at 6 and 24?h. (B) NF-B luciferase activity in wild-type or strains (lysate normalized using OD600 measurements). NF-B luciferase transmission was normalized to transmission from cotransfected luciferase plasmid, and data are displayed as normalized collapse changes from mock-treated BQ-788 samples. (A and B) Data are representative of results from two self-employed ...

Label License, Gaussia luciferase, material transfer license, US Patent, 6232107, 6436682, 6780974, 7045599, 7109315, 7238497, HTS, high throughput screening

The recombinant cell line used in this assay (H1L6.1c2) was generated by stably transfecting the plasmid pGudLuc6.1 into mouse hepatoma (Hepa1c1c7) cells. The pGudLuc6.1 plasmid contains the CYP1A1 dioxin-responsive domain (inclusive of four DREs) upstream of the firefly luciferase gene ...

Control vector for constitutive high-level expression of intracellular green Renilla luciferase under control of the CMV promoter.

A paper published on October 2 in the Journal of Virology describes an exciting development in the world of influenza research-the construction of a luciferase reporter virus that does not affect virulence and can be used to track development and spread of infection in mice.

When I joined the lab team in May I was familiar with lab equipment and testing but I wasnt familiar with luminometers and ATP. I had heard of ATP in my

Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...

Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...

We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate. There is a real need for novel therapeutic approaches, but such advances require a detailed knowledge of C. albicans and its in vivo pathogenesis. Additionally, any novel antifungal drugs against C. albicans infections will need to be tested for their in vivo efficacy over time. Fungal pathogenesis and drug-mediated resolution studies can both be evaluated using non-invasive in vivo imaging technologies. In the work presented here, we used a codon-optimized firefly luciferase reporter system for detecting C. albicans in mice. We adapted the firefly luciferase in order to improve its maximum emission intensity in the red light range (600-700 nm) as well as to improve its thermostability in mice. All non-invasive in vivo imaging of experimental animals was performed with a

3781 In this study, we examined transfection efficiency of naked plasmid DNA using microbubbles (Optison) with ultrasound in vitro and in vivo experiments as the feasibility of a novel nonviral vector system that transfer naked plasmid DNA into a melanoma. First, we tested the feasibility of ultrasound-mediated transfection of naked plasmid DNA in mouse melanoma B16F1 cell lines. Luciferase plasmid mixed with or without Optison was transfected into cultured mouse melanoma cells using ultrasound (1MHz; 0.4 W2) for 1 sec. Interestingly, luciferase activity was dramatically increased in cells treated with Optison, while few luciferase activity could be detected without Optison (P,0.05). We then transfected luciferase plasmid mixed with Optison by means of therapeutic ultrasound into mouse melanoma tumor. Twenty four hours after transfection, luciferase activity, transfected with Optison and ultrasound, was also higher than that of plasmid alone. Finally, we examined the feasibility of therapeutic ...

The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is

FIG 12 HRV16 infection does not block secretion of the Gaussia luciferase reporter. HeLa Ohio cells stably expressing the naturally secreted Gaussia luciferase (HeLa-Gluc) were infected with HRV16 (red squares) at an MOI of 20 for 1 h, followed by culture for the indicated periods of time up to 7 h postinfection (Time hpi). As controls, HeLa-Gluc cells were uninfected (blue circles) or uninfected but treated with BFA (green triangles). The cell culture medium was removed and replaced every 30 min, and the cells were harvested at 1-h intervals. The Gluc activity then was measured in the culture media and cell lysates. The cumulative secretion over time is calculated by adding each successive 30-min culture media sample to the previous total. Panel A shows Gluc activity in the cell lysate, and panel B shows secreted Gluc activity in the cell culture medium. Each point represents the mean relative light units (RLU) (± standard deviations) as a measure of Gluc activity from triplicate assay points ...

Chromosomal aberrations of BCL11A at 2p16.1 have been reported in a variety of B-cell malignancies and its deficiency in mice leads to a profound block in B-cell development. Alternative pre-mRNA splicing of BCL11A produces multiple isoforms sharing a common N-terminus. The most abundant isoform we have identified in human lymphoid samples is BCL11A-XL, the longest transcript produced at this locus, and here we report the conservation of this major isoform and its functional characterization. We show that BCL11A-XL is a DNA-sequence-specific transcriptional repressor that associates with itself and with other BCL11A isoforms, as well as with the BCL6 proto-oncogene. Western blot data for BCL11A-XL expression coupled with data previously published for BCL6 indicates that these genes are expressed abundantly in germinal-center-derived B cells but that expression is extinguished upon terminal differentiation to the plasma cell stage. Although BCL11A-XL/BCL6 interaction can modulate BCL6 DNA binding in

Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research inflence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specifi component of non-thermal nature.. The Opened International University of Human Development Ukraine ...

The attractiveness of secreted luciferases as reporters is a strong stimulus for the investigation and exploitation of new bioluminescent systems. Metridia longa is a small luminous marine copepod (Fig. 3D). The bioluminescence originates as a secretion from epidermal glands located in the head part and abdomen in response to mechanical, electrical, or chemical stimuli. Bioluminescence in Metridia longa may well serve as a defense mechanism against predators; the release of a luminous bolus from the animal is accompanied by rapid swimming that displaces the copepod away from its glowing phantom. This luciferase emits light at a peak of 480 nm with a broad emission spectrum extending to 600 nm. Gaussia luciferase has been cloned, overexpressed in bacteria, and used as a sensitive analytical reporter for hybridization assays and monitoring of cellular expression in culture and in vivo (Tannous et al. 2005). 7 Coelenterazine Dependent Luciferases 9 Fig. 3 Origins of Glow-light reporter genes used ...

The pCMV-GLuc 2 Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive CMV (cytomegalovirus) promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a humanized sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.

Since GARP appears to regulate TGF-1 secretion by T cells and TGF-one is essential for the suppressive perform of Treg cells, we sought to identify mechanisms

Bringing the firefly luciferase gene from Promegas pGL4.10[luc2] vector into a BioBrick compatible form as a sensitive reporter gene. To amplify the signal of the luciferase three different sensitivity tuners are assembled before the luciferase gene. The sensitivity tuners were created in 2007 by the iGEM team from Cambridge and amplify the read-out signal. We also assembled the firefly luciferase behind three different strong constitutive promoters for gathering additional information about this reporter gene (Cambridge, 2007). You can find this BioBrick here: BBa_K389004 You can find this BioBrick with sensitivity tuner 1 here: BBa_K389401 You can find this BioBrick with sensitivity tuner 2 here: BBa_K389402 You can find this BioBrick with sensitivity tuner 3 here: BBa_K389403 You can find this BioBrick under the control of a weak constitutive promoter here: BBa_K389302 You can find this BioBrick under the control of a medium strong constitutive promoter here: BBa_K389307 You can find this ...

D-Luciferin Firefly, free acid 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid Luciferin is a common bioluminescent reporter used for in-vivo imaging of the expression of the luc marker gene . It is the substrate for the Firefly luciferase enzyme which utilizes ATP a ...

Andrew C. Liu is the author of this article in the Journal of Visualized Experiments: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Background Indie luciferase reporter assays and fluorescent translocation assays have already been successfully found in medication discovery for a number of molecular targets. would work for high throughput testing and can determine small substances that hinder FOXO signaling at different amounts. Background Forkhead package O (FOXO) proteins are growing as transcriptional integrators of pathways that regulate a number of cellular procedures, including differentiation, rate of metabolism, tension response, cell routine and apoptosis [1-3]. FOXO transcription elements have been suggested to do something as em real /em tumor suppressors because of the inhibitory results on cell routine and success [4], properties mediated by their binding as monomers to consensus DNA binding sites. Their transcriptional activity is certainly governed with a network of signaling occasions, the best known of which may be the phosphorylation of FOXO proteins at three extremely conserved serine and threonine IL6ST ...

We have studied the PAC-2 cell line extensively 6,7,14,15 and so have determined a set of electroporation conditions that provide optimal transfec-tion

Plasmids. The IFN-γ firefly-luciferase construct was generated by subcloning of specific IFN-γ-specific promoter regions (chr10:118439048-118441048, mm10) into pGL4.27 (Promega). The TEAD reporter, 8xGTIIC, murine Yap, murine Taz, and dominant-negative human TEAD1 (dnTEAD) were all described previously (76).. Cell culture and luciferase assay. HEK293T cells were maintained at 37°C with 5% CO2 in DMEM supplemented with 10% FBS, 1% penicillin, and 1% glutamate and streptomycin. All transfections were completed using FuGENE 6 (Roche). Luciferase experiments included 250 ng IFN-γ firefly-luciferase reporter constructs, 80 ng Yap or Taz, 80 ng dnTEAD, and 75 ng pGL2-Basic-Renilla luciferase (Promega). All transfections maintained an equal concentration of total DNA with transfection of pCMV-Sport6 empty vector (Invitrogen, Thermo Fisher Scientific). Cellular extracts were collected 48 hours after transfection and used in a dual-luciferase assay (Promega). Firefly luciferase activity was ...

Jurkat-Lucia™ NFAT-CD16 cells are human reporter cells for the early nuclear translocation of NFAT upon antibody-dependent cellular cytotoxicity (ADCC) induction. A bioluminescent signal is produced by an NFAT-dependent Lucia luciferase reporter protein.

The cell lysates were collected for luminescence quantification using the protocol DLR-0-INJ (with 10 s integral time) of the GloMaxTM Luminometer (Promega). Idasanutlin manufacturer Ten microliter of each sample was treated with 50 μL of Luciferase Assay Reagent II to obtain the first measurement, while the second measurement was acquired upon addition of 50 μL Stop & Glo® Reagent. The ratio of the first and second luminescence readings was taken as the. level of desired plasmid activation. The Stealth siRNA (Invitrogen) designated S1, S2 or S3 were designed to target human SARM in three different domains. HEK293 cells were seeded into 24-well plates at a density of 1×105 cells/well in 0.5 mL medium, and were transfected with expression vectors and luciferase reporter genes together with siRNA for 24 h. Then the cells were harvested and divided into two halves, one for measurement of SARM mRNA level by end-point PCR and the other for luciferase assay. To examine the effect of LPS ...

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ences derived from the estrogen responsive Complement three (C3) or Metalloproteinase 1 (MMP1) gene. The transfection efficiency was monitored by the

Plasmid pAAV:cTNT::Luciferase from Dr. William Pus lab contains the insert Firefly luciferase and is published in Circ Res. 2014 Jul 18;115(3):354-63. doi: 10.1161/CIRCRESAHA.115.303632. Epub 2014 May 15. This plasmid is available through Addgene.

Name of Products GFP and Luciferase (firefly) Expressing Human Dermal Fibroblasts-Neonatal (GFP-Luc-HDFCs-Neo) Catalogue Number UBP-0008-NeoGFP-Luc Product

Description of a method for the co-transfection of individual 3UTR luciferase reporter constructs with a miRNA mimic. This method uses the LightSwitch Luciferase Assay System and the protocol is efficient, reproducible, and amenable to high-throughput analysis.

Supernatants were preincubated with Protein G Sepharose (GE Health care) for 1 h and anti-Ago2 or control IgG in 4C for 2 h accompanied by addition of 30 l of Protein G Sepharose (GE Health care) for 1 h. (C) Recognition of miR-122 appearance by Northern blot (best -panel) and qRT-PCR (bottom level). Total RNA was extracted from each cell as well as the comparative appearance Mouse monoclonal to CD31 of miR-122 was dependant on qRT-PCR through the use of U6 snRNA as an interior control. (D) miR-122 activity in miR-122-knockout Huh7 cells. pmirGLO vectors having the complementary series of miR-122 beneath the luciferase gene had been transfected into Huh7-122KO and Huh7-122KOR cells. At 48 h post-transfection, the luciferase activity was driven. The info are representative of three unbiased experiments. Mistake bars suggest the typical deviation from the mean and asterisks suggest significant distinctions (**P < 0.01) versus the outcomes for the control.(TIF) ppat.1006374.s001.tif (502K) ...

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I am interested in obtaining luminescence in E. coli in-vivo and would like information on plasmids including sequence and restriction maps. I would like to find someone who has some expertise with luciferase and perhaps has a variety of constructs available. Thanks for your help, David Rosen ...

Luciferase Control RNA from Promega,in vitro Translation, Accessory Products,biological,biology supply,biology supplies,biology product

"Reporter genes have been used for several decades to study regulation of gene... *Hitra in zanesljiva dostava, plačilo tudi po povzetju.*

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ScopesMonkey is a fanfiction author that has written 113 stories for StarTrek: Deep Space Nine, StarTrek: Other, Firefly, and Sherlock.