Lucanthone is orally available thioxanthone-based DNA intercalator and inhibitor of the DNA repair enzyme apurinic-apyrimidinic endonuclease 1 (APEX1 or APE1), with anti-schistosomal and potential antineoplastic activity. Lucanthone intercalates DNA and interferes with the activity of topoisomerases I and II during replication and transcription, thereby inhibiting the synthesis of macromolecules. In addition, this agent specifically inhibits the endonuclease activity of APE1, without affecting its redox activity, resulting in unrepaired DNA strand breaks which may induce apoptosis. Therefore, lucanthone may sensitize tumor cells to radiation and chemotherapy. Furthermore, lucanthone inhibits autophagy through the disruption of lysosomal function. The multifunctional nuclease APE1 is a key component for DNA repair; its expression is often correlated with tumor cell resistance to radio- and chemotherapy. Check for active clinical trials or closed clinical trials using this agent.
Results. Both pemetrexed and lucanthone inhibited tumor growth relative to the vehicles during the 28 days of treatment. For each treatment, fold of increases of the tumor volume were 42, 28, 34 and 19 for vehicles, pemetrexed, lucanthone, and the combination of pemetrexed and lucanthone, respectively. Comparing to the vehicle-treated group, these represented 19%, 33%, and 55% of growth inhibition for groups treated with pemetrexed, lucanthone, and the combination of pemetrexed and lucanthone, respectively. During the 28-day period, the body weight changes excluding the tumor weights were -2.9%, -0.9%, -6.1% and -1.7% for vehicles, pemetrexed, lucanthone, and the combination of pemetrexed and lucanthone, respectively. These body weight data indicated that the treatments were well tolerated with minimum or no general toxicity ...
The Boxwood Blight Cleanliness Program utilizes a systems approach to prevent the spread of boxwood blight via nursery stock. The Boxwood Blight Cleanliness Program is a voluntary program. Participating nurseries agree to use best management practices, developed by the National Plant Board and nursery industry, to reduce the risk of introducing and spreading boxwood blight, which is caused by the fungus Calonectria pseudonaviculata (also known as Cylindrocladium pseudonaviculatum and Cylindrocladium buxicola). The program is coupled with inspections and audits by the Office of Plant Industry Services ...
Introduction. The Rate of Oxygen Consumption of Germinating and Non-Germinating Seeds 2009-03-06 Oxygen consumption in Germinating and Non-germinating Pea Seeds Purpose: To find out and compare the cellular respiration rate at different temperature by using germinating and non-germinating pea seeds. Hypothesis: If the germinating peas are in the cold or the room temperature water, then the rate of cellular respiration will be higher than the rate for the beads or the non-germinating peas. The colder the temperature of the water is, the slower the process of cellular respiration in the peas is. Variables: Controlled: Peas Independent: Temperature, time Dependent: Rate of oxygen consumption Materials: * * Thermometer * Pencil and paper * A water bath * Beads * Germinating Peas * Non-germinating Peas * Beads * Beaker * Ice * Food colouring * Paper towels * Potassium hydroxide, KOH pellets * one-hole test-tube stoppers * Tape * Millimetre rulers * Non-absorbent cottons * Laboratory scoop * 2 Test ...
Abstract The susceptibility of Liberian and Puerto Rican strains of Schistosoma mansoni to antischistosomal drugs was compared. The Liberian strain was more sensitive than the Puerto Rican strain to lucanthone · HCl, SQ 18,506, and stibophen. Parameters tested 90 days after treatment were hatching of eggs, parasitological cures, and extent of worm reductions.
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TY - JOUR. T1 - The isolation of abscisic (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis. AU - Koornneef, M.. AU - Jorna, M.L.. AU - Brinkhorst-van der Swan, D.L.C.. AU - Karssen, C.M.. PY - 1980. Y1 - 1980. M3 - Article. VL - 17. SP - 99. EP - 102. JO - Arabidopsis Information Service. JF - Arabidopsis Information Service. SN - 0066-5657. ER - ...
The genetic variation and structure of Leucomeris decora, an endangered species in China were investigated. Analyses of three chloroplast DNA (cpDNA) regions (the rpl16 intron, trnQ-5 rps16 intergenic spacer and rpl32-trnL intergenic spacer) and one nuclear gene (GAPDH: encoding glyceraldehyde 3-phosphate dehydrogenase) were conducted on 11 L. decora populations. Low levels of cpDNA genetic diversity were found in this species and within populations, with the identification of 2 haplotypes in a total of 2,745 bp, while the level of genetic diversity revealed by the nuclear gene GAPDH was relatively high, indicating that random losses of genetic polymorphisms from populations may have occurred recently. High levels of genetic differentiation among populations for both markers were detected in L. decora, which could be a consequence of the limited gene flow caused by geographic isolation among populations. An analysis of molecular variance revealed at the nuclear locus suggested the presence of ...
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Abstract Schistosomes obtained by perfusion from host animals as early as 2 hours after in vivo treatment with hycanthone and transferred into untreated recipient hamsters died in the recipient host. In contrast, unexposed schistosomes transferred into recipient hamsters treated from 7 days to 36 hours previously showed a normal survival. In vitro treatment of schistosomes with hycanthone concentrations comparable to those used in in vivo studies, followed by transfer of the parasites into normal hamsters, resulted in death of the worms. The time of lethal hycanthone exposure in vitro could be as short as 15 minutes. Hycanthone-resistant schistosomes or immature worms were not affected under similar in vitro conditions. Our data suggest that the schistosomicidal effect of hycanthone is not caused by a host-derived metabolite.
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AWV #14C: In this experiment, you will Measure gas production. Study the effect of temperature on cell respiration. Determine whether germinating peas and non-germinating peas respire. Compare the rates of cell respiration in germinating and non-germinating peas.
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Here we describe a potential new synthetic lethal therapeutic approach for the treatment of AID-expressing B cell malignancies: attenuation of RAD51-mediated HR to inhibit repair of endogenous AID-generated DSBs, culminating in cytotoxicity. This approach uses inherent recombinase activity, rather than systemically administered genotoxic agents, to induce cell-lethal genomic damage. As such, this strategy represents a special case of synthetic lethal therapy. We establish the proof of principle, showing that AID-expressing human CLL cells are hypersensitive to DIDS, a RAD51 inhibitor. Using a combination of primary patient-derived CLLs and genetic mouse models, we establish that DIDS treatment inhibits repair of AID-mediated DSBs in AID-expressing, patient-derived CLL cells. These findings establish that HR attenuation may be a therapeutically viable approach to selectively targeted treatment of cancers (and other pathologies) involving AID-expressing cells. Because AID mRNA expression is found ...
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Measuring Respiration of Germinating and Non-germinating Peas By: Krunal Patel Introduction Living cells require transfusions of energy from outside sources to perform their many tasks - for example, assembling polymers, pumping substances across membranes, moving, and reproducing (Campbell, and Reece 162). Heterotrophs obtains its energy for its cells by eating plants that makes it own food (Autotrophs); some animals feed on other organisms that eat plants. The most beneficial catabolic pathway in an organism is cellular respiration, in which oxygen […]. ...