TY - JOUR. T1 - Cyclosporine reduces basolateral, but not apical, nitric oxide secretion in medullary thick ascending limb cells. AU - Wu, M. S.. AU - Bens, M.. AU - Yu, H. M.. AU - Vandewalle, A.. PY - 2000. Y1 - 2000. N2 - Cyclosporine (CsA) reduces nitric oxide (NO) production in medullary thick ascending limb (mTAL) cells. We postulated that CsA affected NO secretion in a vectorial manner in polarized renal epithelial cells. The experiments were performed in a model of mTAL subcultured cells. The expression of iNOS in mTAL cells was confirmed by RT-PCR. The cells were grown on a non-permeable filter. Nitrite was measured by the modified Griess method. Transepithelial resistance was measured to ensure the integrity of the tight junction. CsA (100 ng/ml) reduced NO production by 22% in mTAL cells. The inhibitory effect was limited to the basolateral side (control: 165 ± 11; plus CsA: 93 ± 17 nM/106 cells, P ,0.001) without affecting apical NO secretion. The transepithelial resistance through ...
TY - JOUR. T1 - Effect of sodium delivery on superoxide and nitric oxide in the medullary thick ascending limb. AU - Abe, Michiaki. AU - OConnor, Paul. AU - Kaldunski, Mary. AU - Liang, Mingyu. AU - Roman, Richard J.. AU - Cowley, Allen W.. PY - 2006. Y1 - 2006. N2 - Hypertension is associated with increased levels of oxidative stress and medullary renal injury. Previous studies have shown that elevations in renal perfusion pressure increase Na+ delivery to the medullary thick ascending limb (mTAL), and enhancement of NaCl transport in the outer medulla has been reported in many experimental forms of hypertension. This study examined the effects of increased Na+ and fluid delivery in mTAL perfused in vitro on the generation of superoxide. Osmolality was maintained constant between low- and high-Na+ perfusates by adjusting with choline Cl-. Real-time fluorescent microscopic techniques were used to determine the generation of superoxide and nitric oxide in individual mTAL cells using ...
Local purinergic signals modulate renal tubular transport. Acute activation of renal epithelial P2 receptors causes inhibition of epithelial transport and thus, should favor increased water and salt excretion by the kidney. So far only a few studies have addressed the effects of extracellular nucleotides on ion transport in the thick ascending limb (TAL). In the medullary thick ascending limb (mTAL), basolateral P2X receptors markedly (~25%) inhibit NaCl absorption. Although this segment does express both apical and basolateral P2Y2 receptors, acute activation of the basolateral P2Y2 receptors had no apparent effect on transepithelial ion transport. Here we studied, if the absence of the P2Y2 receptor causes chronic alterations in mTAL NaCl absorption by comparing basal and AVP-stimulated transepithelial transport rates. We used perfused mouse mTALs to electrically measure NaCl absorption in juvenile (,35 days) and adult (,35 days) male mice. Using microelectrodes, we determined the ...
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We recently reported that the rat thick ascending limb (THAL) possesses an active isoform of nitric oxide synthase (NOS) that is substrate-limited in vitro. NO produced by THAL NOS inhibits chloride flux. Protein and transcript for each of the primar
During the passed few years the cellular mechanisms responsible for the NaCl reabsorption in the thick ascending limb of the Henle loop of mammalian nephron and of the early distal tubule of amphibian
Definition of loop of Henle in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is loop of Henle? Meaning of loop of Henle as a legal term. What does loop of Henle mean in law?
Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern of MT1 mRNA was cortex (CO),outer stripe (OS)=inner stripe (IS)=inner medulla (IM), of MT2 mRNA was IM,CO,IS=OS, and of MT3 mRNA was IM,CO=OS=IM. MT1/2-antibody stained with heterogeneous intensity the cell cytoplasm and nuclei in proximal tubule (PT) and thin ascending limb, whereas MT3-antibody stained weakly the cell cytoplasm in various cortical tubules and strongly the nuclei in all nephron segments ...
We used a mathematical model of O(2) transport and the urine concentrating mechanism of the outer medulla of the rat kidney to study the effects of blood pH and medullary blood flow on O(2) availability and Na(+) reabsorption. The model predicts that in vivo paracellular Na(+) fluxes across medullary thick ascending limbs (mTALs) are small relative to transcellular Na(+) fluxes and that paracellular fluxes favor Na(+) reabsorption from the lumen along most of the mTAL segments. In addition, model results suggest that blood pH has a significant impact on O(2) transport and Na(+) reabsorption owing to the Bohr effect, according to which a lower pH reduces the binding affinity of hemoglobin for O(2). Thus our model predicts that the presumed greater acidity of blood in the interbundle regions, where mTALs are located, relative to that in the vascular bundles, facilitates the delivery of O(2) to support the high metabolic requirements of the mTALs and raises the concentrating capability of the outer ...
I really didnt get my grips with the Loop of henle what is its purpose? is it to maintain osmolality of the interstitial fluid of the medulla, so that they are kinda exchanging salts and water and is that the purpose of urea, to go into the medulla to maintain the osmolality of the medulla...but what is the purpose of that then? What is the purpose of Loop of ...
I really didnt get my grips with the Loop of henle what is its purpose? is it to maintain osmolality of the interstitial fluid of the medulla, so that they are kinda exchanging salts and water and is that the purpose of urea, to go into the medulla to maintain the osmolality of the medulla...but what is the purpose of that then? What is the purpose of Loop of ...
In several previous studies, we used a mathematical model of the thick ascending limb (TAL) to investigate nonlinearities in the tubuloglomerular feedback (TGF) loop. That model, which represents the TAL as a rigid tube, predicts that TGF signal transduction by the TAL is a generator of nonlinearities: if a sinusoidal oscillation is added to constant intratubular fluid flow, the time interval required for an element of tubular fluid to traverse the TAL, as a function of time, is oscillatory and periodic but not sinusoidal. As a consequence, NaCl concentration in tubular fluid alongside the macula densa will be nonsinusoidal and thus contain harmonics of the original sinusoidal frequency. We hypothesized that the complexity found in power spectra based on in vivo time series of key TGF variables arises in part from those harmonics and that nonlinearities in TGF-mediated oscillations may result in increased NaCl delivery to the distal nephron. To investigate the possibility that a more realistic ...
Werner Henle, Mary N. Crawford, Gertrude Henle, Humberto Faz Tabio, Friedrich Deinhardt, Angel Guerra Chabau and Irving J. Olshin ...
Taybi-Linder syndrome (TALS) is a rare autosomal recessive syndrome of as yet unknown etiology. Originally described in 1967 (1), a total of about 30 TALS cases have been reported (2). The TALS phenotype includes marked intrauterine and postnatal growth retardation; short, bowed long bones with severe delay in epiphyseal maturation; severe microcephaly; brain malformations, including pachygyria or agyria; characteristic dysmorphic features; dry skin; and sparse hair [supporting online material (SOM) text and fig. S1] and unexpected death within the first 3 years of life.. We originally mapped TALS to a 13-cM region (D2S2254 to D2S2215, 10 Mb) on chromosome 2q14 (3) in three consanguineous families (families F1 to F3, fig. S2) from the Mediterranean basin using a new homozygosity mapping approach. This approach relied on individual genome-based inbreeding estimates (4) rather than genealogical information, which is often limited. In the present study, we refined the TALS interval to 3.19 Mb by ...
Background Treatment of arterial bifurcation lesions using drug-eluting stents (DES) is now common clinical practice and yet the mechanisms governing drug distribution in these complex morphologies are incompletely understood. It is still not evident how to efficiently determine the efficacy of local drug delivery and quantify zones of excessive drug that are harbingers of vascular toxicity and thrombosis, and areas of depletion that are associated with tissue overgrowth and luminal re-narrowing. Methods and Results We constructed two-phase computational models of stent-deployed arterial bifurcations simulating blood flow and drug transport to investigate the factors modulating drug distribution when the main-branch (MB) was treated using a DES. Simulations predicted extensive flow-mediated drug delivery in bifurcated vascular beds where the drug distribution patterns are heterogeneous and sensitive to relative stent position and luminal flow. A single DES in the MB coupled with large retrograde
Furosemide is a loop diuretic causing fast and short diuresis. It possesses natriuretic effect and also decreases levels of Cl- in blood, increases excretion of K+, Ca2+, Mg2+. Penetrating into a renal tubule in ascending thick limb of Henles loop it blocks re-absorption of Na+ and Cl-. Due to increased excretion of Na+ secondary intensified excretion of water occurs and as well as increased secretion of K+ in distal part of the renal tubule. Simultaneously excretion of Ca2+ and Mg2+ increases. Furosemide is used to treat edema syndrome in patients with chronic heart failure (degree II and III), cirrhosis, diseases of kidneys, acute heart failure (pulmonary edema), brain edema, hypertensive crisis, arterial hypertension and others ...
Furosemide is a loop diuretic causing fast and short diuresis. It possesses natriuretic effect and also decreases levels of Cl- in blood, increases excretion of K+, Ca2+, Mg2+. Penetrating into a renal tubule in ascending thick limb of Henles loop it blocks re-absorption of Na+ and Cl-. Due to increased excretion of Na+ secondary intensified excretion of water occurs and as well as increased secretion of K+ in distal part of the renal tubule. Simultaneously excretion of Ca2+ and Mg2+ increases. Furosemide is used to treat edema syndrome in patients with chronic heart failure (degree II and III), cirrhosis, diseases of kidneys, acute heart failure (pulmonary edema), brain edema, hypertensive crisis, arterial hypertension and others ...
Furosemide is a loop diuretic causing fast and short diuresis. It possesses natriuretic effect and also decreases levels of Cl- in blood, increases excretion of K+, Ca2+, Mg2+. Penetrating into a renal tubule in ascending thick limb of Henles loop it blocks re-absorption of Na+ and Cl-. Due to increased excretion of Na+ secondary intensified excretion of water occurs and as well as increased secretion of K+ in distal part of the renal tubule. Simultaneously excretion of Ca2+ and Mg2+ increases. Furosemide is used to treat edema syndrome in patients with chronic heart failure (degree II and III), cirrhosis, diseases of kidneys, acute heart failure (pulmonary edema), brain edema, hypertensive crisis, arterial hypertension and others ...
Furosemide is a loop diuretic causing fast and short diuresis. It possesses natriuretic effect and also decreases levels of Cl- in blood, increases excretion of K+, Ca2+, Mg2+. Penetrating into a renal tubule in ascending thick limb of Henles loop it blocks re-absorption of Na+ and Cl-. Due to increased excretion of Na+ secondary intensified excretion of water occurs and as well as increased secretion of K+ in distal part of the renal tubule. Simultaneously excretion of Ca2+ and Mg2+ increases. Furosemide is used to treat edema syndrome in patients with chronic heart failure (degree II and III), cirrhosis, diseases of kidneys, acute heart failure (pulmonary edema), brain edema, hypertensive crisis, arterial hypertension and others ...
The growth arrest specific 1 (gas1) gene is highly expressed in quiescent mammalian cells. Overexpression of gas1 in normal could inhibit G(0)/G(1) transition. The spatial-temporal expression patterns for gas1 were established in 8.5- to 14.5-day-old embryos by immunohistochemical staining and in situ hybridization. Gas1 was found heterogeneously expressed in [the] limb. The antiproliferative effects of gas1 on 10.5 and 12.5 day limb cells were investigated by flow cytometry. In 10.5 day limbs cells, gas1 overexpression could not prevent G(0)/G(1) progression. Gas1 could only induce growth arrest if p53 was also coexpressed {p53 was not coexpressed significantly in LSJL}. In contrast, gas1 overexpression alone was able to induce growth arrest in 12.5 day limb cells. We also examined the cell cycle profile of gas1-expressing and nonexpressing cells by immunochemistry and flow cytometry. For 10.5 day Gas1-expressing limb cells, we did not find these cells preferentially distributed at G0/G1, as ...
Physiology Test Question - In the epithelial cells of the kidney loop of Henle, the Na+/K+ ATPase is localized to the __________ membrane
La nostra taula de treball dins de les VIII Jornades Escoles 0-12 estava dedicada als nens i a les nenes com a tals, és a dir, com a nens i com a nenes que són. A lhora de plantejar-nos com podíem abordar la temàtica, ens ...
High consistency screening applications for efficient thick stock screening for broke screening, thick stock screening and protection screening before refining
Insulin-like growth factor-I (IGF-I) modulates renal function, growth, and repair. IGF-I produced in the kidney is one component of the intrarenal IGF-I system comprising the IGF-I receptor (IGF-IR) and six IGF-binding proteins (IGFBP). Because of the physiologic importance of IGF-I and its potential therapeutic properties, the renal sites of mRNA synthesis for IGF-I, IGF-IR, and IGFBP-I through IGFBP-5 were characterized in rat kidney by in situ hybridization. Anatomical heterogeneity was prominent. IGF-I mRNA was present in the thick ascending limb of Henle in the outer medulla, whereas IGF-IR mRNA was diffusely present at low levels throughout the kidney. IGFBP-I mRNA was localized to cells within the distal convoluted tubules as well as the thick ascending limb of Henle. IGFBP-2 mRNA was expressed in glomeruli, medullary ray collecting ducts, pelvic smooth muscle and uroepithelium, and the papilla tip; IGFBP-3 mRNA was localized to the cortical interstitium, whereas IGFBP-4 mRNA was ...
Henle was born in Fürth, Bavaria, to Simon and Rachel Diesbach Henle (Hähnlein). He was Jewish.[3] After studying medicine at Heidelberg and at Bonn, where he took his doctors degree in 1832, he became prosector in anatomy to Johannes Müller at Berlin. During the six years he spent in that position he published a large amount of work, including three anatomical monographs on new species of animals and papers on the structure of the lymphatic system, the distribution of epithelium in the human body, the structure and development of the hair, and the formation of mucus and pus.[4] In 1840, he accepted the chair of anatomy at Zürich and in 1844 he was called to Heidelberg, where he taught anatomy, physiology, and pathology. About this period he was engaged on delineating his complete system of general anatomy, which formed the sixth volume of the new edition of Samuel Thomas von Sömmerings treatise, published at Leipzig between 1841 and 1844. While at Heidelberg he published a zoological ...
INTERNET-DRAFT DTLS as a Transport Layer for RADIUS 9 October 2013 The above paragraph can be rephrased more generically. A session MUST be deleted when non-RADIUS traffic is received over it. This specification is for RADIUS, and there is no reason to allow non- RADIUS traffic over a RADIUS/DTLS connection. A session MUST be deleted when RADIUS traffic fails to pass security checks. There is no reason to permit insecure networks. A session SHOULD NOT be deleted when a well-formed, but "unexpected" RADIUS packet is received over it. Future specifications may extend RADIUS/DTLS, and we do not want to forbid those specifications. Once a DTLS session is established, a RADIUS/DTLS server SHOULD use DTLS Heartbeats [RFC6520] to determine connectivity between the two servers. A server SHOULD also use watchdog packets from the client to determine that the connection is still active. As UDP does not guarantee delivery of messages, RADIUS/DTLS servers which do not implement an application-layer watchdog ...
Detective Nephron, world-renowned for expert analytical skills, trains budding physician-detectives on the diagnosis and treatment of kidney diseases. L.O. Henle, a budding nephrologist, presents a new case to the master consultant. Nice Glom (the new medical student) enters the room along with L.O. Henle to present a case. Nephron What do you have for me today Henle? Henle
By Mike Henle www.mikehenle.com LAUGHLIN, Nev., -Off-road racers have no reason to slow down as the Christmas season continues. As it has been the case […]. Read More. ...
You (Yours Truly) dont have an Arachnoid Cyst. You just dont have a lot of brain matter in the right side of your head.". Dr. Stuart S. Kaplan. ...
Looking for Descending Limb? Find out information about Descending Limb. The U-shaped portion of a renal tubule formed by a descending and an ascending tubule. Also known as Henles loop Explanation of Descending Limb
Angelika Bierhaus, Stephan Schiekofer, Markus Schwaninger, Martin Andrassy, Per M. Humpert, Jiang Chen, Mei Hong, Thomas Luther, Thomas Henle, Ingrid Klöting, Michael Morcos, Marion Hofmann, Hans Tritschler, Bernd Weigle, Michael Kasper, Mark Smith, George Perry, Ann-Marie Schmidt, David M. Stern, Hans-Ulrich Häring, Erwin Schleicher and Peter P. Nawroth ...
Biology Assignment Help, Physical state of protoplasm, PHYSICAL STATE OF PROTOPLASM Several theories have been given about its physical structure - (i ) Granular Theory (Proposed by Altman, Hanstein, 1886) - Granules embedded in a fluid, those are known as bioplast/cytoplast . Henle & Meg
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Guide to weekend getaways from Tal Chhapar. All you need to know about 9 places to visit near Tal Chhapar, distance from Tal Chhapar, things to do in Tal Chhapar getaways, reviews, locations.
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
Certainly when the almost urine got to distal tubule 85% of the water had been already reabsorbed and all that had to be done was to finish up this process. In fact only 1% was supposed to go beyond the rather uniform looking cells of the distal tubule.. Gradually, however, our views began to change and through the advances in physiology, biochemistry and histology a different picture began to emerge. Nobody could have dreamed of the variety of cells in both the distal tubule and the collecting system. In a healthy person or animal, things seemed simple indeed, but let a defect or two creep in and then we begin to see the dragons.. We already understand quite a bit about what can happen. If we go back one page (to page 11) we note that the very first part of the distal tubule has cells with the characteristics of those of the thick ascending loop of Henle. These cells are marked in yellow and include the macula densa. A defect in these cells leads to Bartter s syndrome.. Next, come the cells (in ...
How does Lasix work and what pharmaceutical group does it belong to?. Lasix is a diuretic agent. Lasix causes a short term diuresis. Lasix action mechanism is associated with the reabsorbtion block of chloride and sodium ions in the Henles loop.Lasix works by increasing excretion of K+, Ca2+, Mg2+ out of the organism. Hypotensive effect of the medication is associated with increased elimination of sodium and water. Which conditions are treated with Lasix?. Lasix is used for the treatment of the following conditions: ...
How does Lasix work and what pharmaceutical group does it belong to?. Lasix is a diuretic agent. Lasix causes a short term diuresis. Lasix action mechanism is associated with the reabsorbtion block of chloride and sodium ions in the Henles loop.Lasix works by increasing excretion of K+, Ca2+, Mg2+ out of the organism. Hypotensive effect of the medication is associated with increased elimination of sodium and water. Which conditions are treated with Lasix?. Lasix is used for the treatment of the following conditions: ...
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1. The Na+ and Cl− transport systems of human erythrocytes have been compared for their sensitivities to diuretics known to act in the ascending limb of Henles loop. In addition, chemical analogues of loop compounds and also diuretics which act in other areas of the nephron have been examined.. 2. The Na+ transport system lacks specificity with respect to inhibition by loop diuretics and also a related chemical analogue studied at equivalent concentrations.. 3. The Cl− transport system is inhibited, at low concentrations, by diuretics known to act in the ascending limb of Henles loop.. 4. Erythrocyte Cl− transport offers a useful model with which to study the biochemical action of diuretics.. ...
From NCBI Gene:. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in ...
The case report presented here illustrates the benefits of weight reduction on the progression of kidney disease. There are few studies investigating the pathophysiology of obesity and its early effects on kidney structure and function. Clinical as well as laboratory animal studies have suggested the role of glomerular hypertension due to renal vasodilatation and increase in hydrostatic pressure leading to increased glomerular wall stress and increased tubular sodium absorption [10, 11]. The other proposed mechanism of excessive tubular sodium re-absorption include increased intra-renal pressures caused by the excess accumulation of adipose tissue in the viscera with compression of the loop of Henle and vasa recta leading to sluggish flow in the renal tubules and vasa recta and thus causing an increase in the tubular sodium re-absorption [9, 12]. Increased sodium re-absorption in the loop of Henle initially reduces the macula densa sodium chloride delivery thereby initiating a macula densa ...
Find SLC12: Electroneutral Cation-coupled Cl Cotransporter Family research area related information and SLC12: Electroneutral Cation-coupled Cl Cotransporter Family research products from R&D Systems. Learn more.
... , world renowned for expert analytic skills, trains budding physician-detectives on the diagnosis and treatment of kidney diseases. Wildly waving a stack of paper records, budding nephrologist L.O. Henle and medical student Ms. Curious Tubule run down the hall toward Detective Nephrons office.. Henle (with a smile): A case for you sir!. The detective sits facing the window. He is observing a mob outside his office with his coffee mug in hand. ...
GO:0032835 glomerulus development --, regulation of glomerulus development GO:NEW --,negative regulation of glomerulus development GO:NEW --,positive regulation of glomerulus development GO:NEW Also as an extension to SF#2912058 requested 10/12/09 GO:0001658 branching involved in ureteric bud morphogenesis --,regulation of branching involved in ureteric bud morphogenesis GO:NEW --,negative regulation of branching involved in ureteric bud morphogenesis GO:NEW --,positive regulation of branching involved in ureteric bud morphogenesis GO:NEW SF 2914121 [YAF 17/12/09] David Added: regulation of branching involved in ureteric bud morphogenesis ; GO:0090189/ positive regulation of branching involved in ureteric bud morphogenesis ; GO:0090190/ negative regulation of branching involved in ureteric bud morphogenesis ; GO:0090191 regulation of glomerulus development ; GO:0090192/ positive regulation of glomerulus development ; GO:0090193/ negative regulation of glomerulus development ; GO:0090194. ...
Torsemide is a diuretic of the pyridine-sulfonylurea class. Its chemical name is 1-isopropyl-3-[(4-m-toluidino-3-pyridyl) sulfonyl] urea. Torsemide acts from within the lumen of the thick ascending portion of the loop of Henle, where it inhibits the Na/K+/2CI- carrier system. ...
..........o0O0o.......... More resources here: Best kidney animation ever from biologymad.com Quick guide to thekidney from kidneypatientguide.org.uk Complete tutorial from Sumanas Very detailed animations from University of British Columbia Bilingual (Chinese and English) guide to the kidney, with rave tunes from hkedcity.net More Loop of Henle action from the University of Colorado Hormonal control of ADH…
I never had a problem learning about diuretics and the ascending loop of Henle, but every after market accessory I purchase, never seems to work or fit right with what I own. Im waiting a week for the rain and humidity to go away so I can finish the driveway, I decided instead of another day of doing nothing, Ill install the wolverine jig on my grinder. simple, 6 screws, 1 hour tops, 2 hours tops, so I discovered the mounting holes on the grinder are in the way of the wolverine jig, seems
Quinze opérateurs portuaires (dont deux femmes) de la communauté portuaire de Dakar bénéficieront dune formation portuaire du programme TrainForTrade de la CNUCED portant sur « le fonctionnement dun système portuaire » et « les principaux enjeux du futur ». La diffusion des cours sera assurée par des instructeurs locaux tous experts portuaires formés à la méthodologie TrainForTrade. Il sagit de la 9e promotion. 127 cadres portuaires ont déjà été formés à Dakar ...
Thick Concavity Slides available at LabScientific. Made of 3mm thick clear glass, slides are available with single, double or triple concavities.