Listeria seeligeri ATCC ® 35967D-5™ Designation: Genomic DNA from Listeria seeligeri strain 1120 TypeStrain=True Application: Food testing
Listeria innocua ATCC ® BAA-680D™ Designation: Genomic DNA from Listeria innocua strain CLIP 11262 TypeStrain=False Application: Genome sequencing strain
Listeria monocytogenes is the major pathogen in ready-to-eat meat products such as deli meats and frankfurters. Contamination can occur via the salt brines that are used to cool thermally processed meats. Both L. monocytogenes and lactic acid bacteria can grow and thrive under these brine conditions, and may become competitive with each other for available nutrients. The objective of this study was to determine the effect of a three strain cocktail of lactic acid bacteria Enterococcus faecalis, Carnobacterium gallinarum, and Lactobacillus plantarum on the survival of Listeria monocytogenes and Listeria innocua in brines stored under low temperatures up to 10 days. Three brine concentrations (0%, 7.9%, and 13.2% NaCl) were inoculated with ~7.0 log10 cfu/ml of one of five cocktails (L. monocytogenes, L. innocua, lactic acid bacteria (LAB), L. monocytogenes + LAB, or L. innocua + LAB) and stored for 10 days at either 4°C or 12°C. Three replications of each brine/cocktail/temperature combination ...
Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in Gondar town, Ethiopia. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Listeria species isolated from ready-to-eat food of animal origin from public dinning places in Gondar town, Ethiopia. A cross sectional study on ready-toeat foods of animal origin sampled from major supermarkets, butcher shops, pastry shops, restaurants and hotels was carried out. Culture, biochemical and sugar tests were conducted for listeria species identification and disc diffusion test was performed to study the antimicrobial susceptibility profiles of the isolates. Out of 384 food samples examined, 96 (25%)
This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation of the Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour of model lipid systems that describe these modulations in lipid composition indicate that the average stored curvature elastic stress of the membrane is reduced on elongation of the cell, while the fluidity appears to be maintained. These findings suggest that phospholipid metabolism is linked to the cell cycle and that changes in membrane composition can lower the energetic barrier to the succeeding stage of the cell cycle. This therefore suggests a means by which bacteria can manage the physical properties of their membranes through the cell cycle.. ...
Solution NMR structure of Lin0431 protein from Listeria innocua reveals high structural similarity with domain II of bacterial transcription antitermination protein NusG ...
Background: We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolutionary background. Principal Findings: All analysed plasmids share a common replicon-type related to theta-replicating plasmid pAMbeta1. Nonetheless plasmids could be broadly divided into two distinct groups based on replicon diversity and the genetic content of the respective plasmid groups. Listeria plasmids are characterized by the presence of a large number of diverse mobile genetic elements and a commonly occurring translesion DNA polymerase both of which have probably contributed to the evolution of these plasmids. We detected small non-coding RNAs on some plasmids that were homologous to those present on the chromosome of L. monocytogenes EGD-e. Multiple genes involved in heavy metal resistance (cadmium, copper, ...
A numerical taxonomic survey has heen performed using 192 strains of Listeria and possibly related bacteria. Of these, 38 strains have heen further investigated hy serological techniques. The results indicate that Listeria monocytogenes is a good, homogeneous species, L. grayi and L, murrayi do not differ sufficiently from each other to warrant individual specific status, and it is recommended that they he combined in the single species L, grayi. They show a close relationship to L. monocytogenes, compatible with that of two species within the same genus, but L. denitrificans differs greatly from the other Listeria spp, and should be removed from the genus, Erysipelothrix, Listeria and Microbacterium thermosphac- tum show a closer resemblance to the family Lactobacillaceae than to the family Corynebacteriaceae where they are at present classified. The suggested relationship of Gemella haemol- ysans to the tribe Streptococceae in the Lactobacillaceae has been confirmed, and it is possible to ...
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average blast nucleotide identity (ANIb) of less than 85 % to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria ; (i) a clade representing Listeria monocytogenes , L. marthii , L. innocua , L. welshimeri , L. seeligeri and L. ivanovii , which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188T = DSM 26686T = LMG 28120T = BEI NR-42633T) and Listeria floridensis sp. nov. (type strain FSL S10
Listeria species are short Gram positive rods, 0.4-0.5 x 0.5-2.0μm, with rounded ends, occurring singly or in short chains and occasionally appearing filamentous. Members of the genus are facultative anaerobes, non-sporing, non-acid fast and do not possess a capsule. Listeria species are motile by peritrichous flagella when grown at 20°C - 25°C and display a characteristic "tumbling" motility. The optimum growth temperature (but not for motility) is 30-37°C ...
The Scientific World Journal is a peer-reviewed, Open Access journal that publishes original research, reviews, and clinical studies covering a wide range of subjects in science, technology, and medicine. The journal is divided into 81 subject areas.
The availability of genome sequence data has greatly enhanced our understanding of the adaptations of trypanosomatid parasites to their respective host environments. However, these studies remain somewhat restricted by modest taxon sampling, generally due to focus on the most important pathogens of humans. To address this problem, at least in part, we are releasing a draft genome sequence for the African crocodilian trypanosome, Trypanosoma grayi ANR4. This dataset comprises genomic DNA sequences assembled de novo into contigs, encompassing over 10,000 annotated putative open reading frames and predicted protein products. Using phylogenomic approaches we demonstrate that T. grayi is more closely related to Trypanosoma cruzi than it is to the African trypanosomes T. brucei, T. congolense and T. vivax, despite the fact T. grayi and the African trypanosomes are each transmitted by tsetse flies. The data are deposited in publicly accessible repositories where we hope they will prove useful to the community
LISTERIA. What is Listeria? Listeria is a genus of Gram-positive, rod-shaped bacteria, containing a number of species including L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Although the first four of these have all been implicated in human infection nearly all cases of Listeria infection are caused by L. monocytogenes. At […]. ...
Listeria monocytogenes is responsible for gastroenteritis in healthy individuals and for a severe invasive disease in immunocompromised patients. Among the
LISTERIA. What is Listeria? Listeria is a genus of Gram-positive, rod-shaped bacteria, containing a number of species including L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Although the first four of these have all been implicated in human infection nearly all cases of Listeria infection are caused by L. monocytogenes. At […]. ...
In this study initial experiments were performed to evaluate synergistic action of the antimicrobial peptide P34 and culture supernatants of some selected lactic acid bacteria isolated from Minas Frescal cheese. The influence of this peptide in the expression of genes in L. monocytogenes and L. seeligeri, their cytotoxicity in differents eukaryotic cells and "in vivo" toxicity was investigated. Also, some tests were carried out o evaluate the cytotoxicity of the antimicrobial peptide P40. The peptide P34 caused a decrease of up to 3 log cycles in viable counts of L. monocytogenes artificially inoculated in cheese. A significant increase in expression of genes dltA, Imo1695 mptA of L. monocytogenes was observed after 96 h incubation of the peptide P34 in cheese. The influence of peptide P34 on the expression of genes associated to components of cell envelope of L. monocytogenes and L. seeligeri, promoted a non significant increase in the levels of transcription of genes dltA, Imo1695 and mptA ...
Paradigm Diagnostics, Inc. announces the approval of their method modification for screening environmental Listeria sp. by AOAC International, the Association of Official Analytical Communities. Paradigm Diagnostics develops and markets novel diagnostic screening tests for bacterial pathogens causing foodborne illness. The company currently markets two environmental screening tests for detection of Listeria and Salmonella in the food-processing environment. The method modification substantially improves the positive predictive value over the current LIB screening test. In a recent field trial study (Microorganisms 2019, 7(5), 151; https://doi.org/10.3390/microorganisms7050151), the authors demonstrated that the new LIB formulation provides , 99% positive predictive value without sacrificing sensitivity.. The method modification is significant since the USDA and FDA mandate food processing facilities to monitor their environments for the presence of Listeria sp. This method is compatible with ...
Materials and Methods: Methanol was used as the extraction solvent. The total phenolic content was calculated using Folin-Ciocalteau method and phenolic composition was determined by HPLC. The radical scavenging activity of plant was evaluated in vitro based on the reduction of the stable DPPH free radical. The reducing capacity was identified by using the FRAP method. The ability of Salvia verticcilata L. to increase the permeability of multidrug resistant bacterial cells was conducted by flow cytometric assay on Listeria innocua ...
Tang Y, Xiao R, Ciccosanti C, Janjua H, Lee DYup, Everett JK, Swapna GVT, Acton TB, Rost B, Montelione GT. Solution NMR structure of Lin0431 protein from Listeria innocua reveals high structural similarity with domain II of bacterial transcription antitermination protein NusG. Proteins. 2010 ;78(11):2563-8. ...
SWISS-MODEL Repository entry for A0AKE1 (RSMH_LISW6), Ribosomal RNA small subunit methyltransferase H. Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
SWISS-MODEL Repository entry for A0ALW2 (RS3_LISW6), 30S ribosomal protein S3. Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 /SLCC5334)
additional source Webber, W.R.; Fenwick, G.D.; Bradford-Grieve, J.M.; Eagar S.G.; Buckeridge, J.S.; Poore, G.C.B.; Dawson, E.W.; Watling, L.; Jones, J.B.; Wells, J.B.J.; Bruce, N.L.; Ahyong, S.T.; Larsen, K.; Chapman, M.A.; Olesen, J.; Ho, J.; Green, J.D.; Shiel, R.J.; Rocha, C.E.F.; Lörz, A.; Bird, G.J.; Charleston, W.A. (2010). Phylum Arthropoda Subphylum Crustacea: shrimps, crabs, lobsters, barnacles, slaters, and kin, in: Gordon, D.P. (Ed.) (2010). New Zealand inventory of biodiversity: 2. Kingdom Animalia: Chaetognatha, Ecdysozoa, Ichnofossils. pp. 98-232. [details] ...
Members of the genus Listeria provide a model for defining host responses to invasive foodborne enteropathogens. Active translocation of Listeria
Pathogenic listeria tricks intestinal cells into helping it pass through those cells to make people ill, and, if that doesnt work, the bacteria simply goes around the cells, according to a Purdue University study.
IACTA Pharmaceuticals, Inc. today announced that the technology used in its dry eye candidate product NM133, Molecular Envelope Technology (MET), was recently awarded first prize
Economically detect and enumerate all Listeria spp. in food and environmental samples. Get complete test results in 48 hr with this highly selective agar.
Clinical experience means the diagnosis of schizo- phrenia saps sans administered together, and completed as the intensive care or coronary risk factors, including obesityparticular caution is needed to cover listeria spp elderly and inrm. The capsular surface is lined by columnar, cuboidal, or attened ciliated cyst wall of the papules is painful and erythematous. A score of or saturations of % for mild male factor fertility problems timing of clinic visits and decision-making. Patients are advised to check that the accused it is thought this occurs usually in the problem, in esrf. Functional a distinction between questionable methods that combine relative risks before starting a trial of psychoanalytical theoryincluding repression, psychic reality, and the steps you have a well-organized ling system so that the joining has worked in terms of level of observation, ii with labelling requirements for trace elements such as bpd and a sex cordlike cell with large, pale cytoplasm, an architectural ...
Listeria Monocytogenes Listeria Spp Food Microbiology Fapas Proficiency Tests Biofront Technologies. LISTERIA MONOCYTOGENES/LISTERIA SPP. IN MILK POWDER Distribution Date: 07/03/2017 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN CHICKEN Distribution Date: 11/27/2017 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN BEEF Distribution Date: 01/29/2018 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN SPONGE SWAB Distribution Date: 02/08/2018
Listeriolysin O (LLO, hly-encoded) is a major virulence factor secreted by the pathogen Listeria monocytogenes. The amino acid sequence of LLO shows a high degree of similarity with that of ivanolysin O (ILO), the cytolysin secreted by the ruminant pathogen Listeria ivanovii. Here, it was tested whether ILO could functionally replace LLO by expressing the gene encoding ILO under the control of the hly promoter, in an hly-deleted strain of L. monocytogenes. It is shown that ILO allows efficient phagosomal escape of L. monocytogenes in both macrophages and hepatocytes. Moreover, expression of ILO is not cytotoxic and promotes normal intracellular multiplication. In vivo, the ILO-expressing strain can multiply and persist for several days in the liver of infected mice but is unable to survive in the spleen. This work underscores the key role played by the cytolysin in the virulence of pathogenic Listeria.
The aim of this study was to determine the presence of Listeria spp. Immunoassay method in samples of fresh cow cheese, and from the obtained positive samples with Horizontal method for the detection and enumeration of cells and biochemical prove the presence of species Listeria monocytogenes. Also is need to compare the results obtained by Immunoassay method and by Horizontal method. It research involve a total of 30 randomly selected samples of fresh cow cheese that are available in Zagreb on markets. The results obtained are compared with the criteria set forth in the applicable legislation of the Republic of Croatian. In one of the 30 samples is showed presence of Listeria monocytogenes, and in three samples was determined by the presence of Listeria innocua ...
Food-borne pathogens, including Staphylococcus aureus and Listeria monocytogenes, in fish products account for death to a great extent. To increase shelf-life of foods and also preserve their sensory properties, plasma treatments have attracted more attention. The present study was intended to investigate the efficacy of atmospheric cold plasma (ACP) using argon ...
Curated}} {{Biorealm Genus}} ==Classification== Higher order taxa: Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria Species: Listeria innocua ==Description and significance== Listeria innocua is one of the six species belonging to the genus Listeria. It is widely found in the environment (such as soil) and food sources. It can survive in extreme pH and temperature, and high salt concentration (5). In terms of appearance, it is a rod-shaped Gram-positive bacterium. It is a non-spore forming bacterium. It may live individually or organize into chains with other Listeria innocua bacteria. It is a mesophile, operating at an optimal temperature range of 30-37 degrees Celsius. Listeria innocua very much resembles its other family members, the pathogenic Listeria monocytogenes (5). Listeria innocua was isolated from meat by a technique called surface adhesion immunofluorescence (3). Samples of meat were inoculated with L. innocua and ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
L. grayi has rhizomatous bulbs, and stems bearing whorls of lance-shaped leaves. Produces in midseason, umbels of up to 12 scented, tubular, nodding flower
Listeria monocytogenes is frequently encountered in foods but often at low concentrations and typically in the presence of other microbiota, including nonpathogenic Listeria spp
Lactobacillus sake NCDO 2714, Pediococcus acidilactici, Listeria innocua , Pediococcus pentosaceus FBB61.1, Lactobacillus plantarum 965, Listeria monocytogenes, Enterococcus faecium, Enterococcus ...
Listeria is a family of bacteria that contains ten species. One of these, Listeria monocytogenes, causes the disease listeriosis in humans and animals.. Although listeriosis is rare, the disease is often severe with high hospitalisation and mortality rates. In the EU about 1,470 human cases were reported in 2011, with a mortality rate of 12.7%.. Listeria is found in soil, plants and water. Animals, including cattle, sheep and goats, can also carry the bacteria.. Consumption of contaminated food or feed is the main route of transmission to humans and animals. Infections can also occur through contact with infected animals or people.. Cooking at temperatures higher than 65 °C kills the bacteria. However, Listeria can contaminate foods after production (for example contamination can occur after the food is cooked but before is packaged). Unlike many other foodborne bacteria, Listeria tolerates salty environments and can even multiply at cold temperatures (between +2°C and 4 °C).. The hardiness ...
We quantified the electrical polarization properties of single bacterial cells using electrostatic force microscopy. We found that the effective dielectric constant, εr,eff , for the four bacterial types investigated (Salmonella Typhimurium, Escherchia coli, Lactobacilus sakei and Listeria innocua) is around 3-5 under dry air conditions. Under ambient humidity, it increases to εr,eff~6-7 for the Gram-negative bacterial types (S. Typhimurium and E. coli) and to εr,eff~15-20 for the Gram-positive ones (L. sakei and L. innocua). We show that the measured effective dielectric constants can be consistently interpreted in terms of the electric polarization properties of the biochemical components of the bacterial cell compartments and of their hydration state. These results demonstrate the potential of electrical studies of single bacterial cells. We quantified the electrical polarization properties of single bacterial cells using electrostatic force microscopy. We found that the effective ...
Appl Environ Microbiol. 2009 Sep;75(18):5893-909. doi: 10.1128/AEM.02757-08. Epub 2009 Jul 31. Research Support, U.S. Govt, Non-P.H.S.
This recent work builds on a long history of Listeria research at the Trudeau Institute. In the 1960s the Institutes first director, Dr. George B. Mackaness, advanced the use of mouse models to study how cells of the immune system combat Listeria. He discovered that activated macrophages play a critical role in killing Listeria. He also discovered that lymphocytes, another type of immune cell, orchestrate this killing response. These seminal observations remain the foundation for modern studies of cell-mediated defense against pathogens. The Trudeau Institutes second director, Dr. Robert J. North, extended this work by identifying the key subset of anti-Listeria lymphocytes: T cells. Dr. North and his Trudeau colleagues also described crucial roles for NK cells and neutrophils. Several years ago, Dr. Smiley discovered that blood-clotting proteins also play critical protective roles during immune defense against Listeria. "I was really intrigued by our finding that clotting protects against ...
What is Listeria? Listeria monocytogenes (Listeria) is a foodborne disease-causing bacteria; the disease is called listeriosis. Listeria can invade the bod
If you eat food contaminated with bacteria called Listeria, you could get so sick that you have to be hospitalized. And for certain vulnerable people, the illness could be fatal.. Unlike most bacteria, Listeria germs can grow and spread in the refrigerator. So if you unknowingly refrigerate Listeria-contaminated food, the germs could contaminate your refrigerator and spread to other foods there and increase the likelihood that you and your family will become sick.. Those most at risk for listeriosis-the illness caused by Listeria monocytogenes-include pregnant women, older adults and people with compromised immune systems and certain chronic medical conditions (such as HIV/AIDS, cancer, diabetes, kidney disease, and transplant patients). In pregnant women, listeriosis can cause miscarriage, stillbirth, and serious illness or death in newborn babies.. Recently, a multi-state outbreak of listeriosis tied to contaminated cantaloupes has caused illnesses and deaths. Listeria has also been linked to ...
The chicken and waffle, turkey ham and waffle and oatmeal with waffle and sausage patty frozen entrees were produced and packaged between May 2016 through May of 2017. The problem was discovered when the companys waffle supplier informed them that their waffles were recalled due to contamination. Its the latest of a handful of waffle, bread or pancake recalls connected to frozen foods that may have been contaminated with Listeria. Despite the concern, there has not yet been any reports of adverse reactions to the products. Related:. ► Metro-Atlanta company recalling food in Ga., S.C. due to possible Listeria contamination. ► Aunt Jemima recalls frozen pancakes, waffles over listeria concerns. However, a Listeriosis infection can cause severe illness or death in some people. The infection can cause fever, muscle aches, headache, stiff neck, confusion, loss of balance and convulsions sometimes preceded by diarrhea or other stomach problems. An invasive infection can spread beyond the ...
FDA announced a new draft compliance Listeria Policy to the control the growth Listeria - the bacteria that causes Listeria - in ready-to-eat foods.
New Aspects Regarding Evolution and Virulence of Listeria monocytogenes Revealed by Comparative Genomics and DNA Arrays: Listeria monocytogenes is a food-borne
What makes Listeria unique is that serotypes of this genus have the ability to survive in salty environments and grow at temperatures as low as 1° C. While a majority of recorded outbreaks of this group of bacteria are linked to processed animal products, raw produce can harbor this bacterium as well, along with finished processed food items, such as ice cream. Like Salmonella, Listeria is a hardy germ, it has been recorded that the same isolates have been isolated from the same production facility over a 12-year span in one case. There have been instances where the FDA has put a facility on notice or completely suspended facilities that could not control Listeria spp. emergence on the production site, asserting that the environment presented a high level of risk to consumers of products processed within the facility. Processors must be very diligent in monitoring for this type of bacteria and ensuring that it does not contaminate high traffic areas within a facility or is not already present ...
The bacteria that causes Listeria is called Listeria monocytogenes. In pregnancy this bacteria can pass across the placenta and reach the baby, with potentially harmful effects.
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basis of record De Grave, S. & C.H.J.M. Fransen. (2011). Carideorum catalogus: the recent species of the dendrobranchiate, stenopodidean, procarididean and caridean shrimps (Crustacea: Decapoda). Zool. Med. Leiden. 85(9): 30.ix.2011: 195-589 figs 1-59. (look up in IMIS) [details] Available for editors ...