A simple and rapid microextraction procedure based on dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-flame ionization detection (GC-FID) was developed for the extraction and analysis of 2-phenylethanol in rose water sample. In the proposed approach, carbon tetrachloride and ethanol were used as extraction and dispersive solvents, respectively. Some important parameters, such as extraction and disperser solvent and volume of them, extraction time, pH and salt effect were investigated. Under optimized conditions, a linear relationship was obtained in the range of 1.0-300.0 mg l-1. The method detection limit was 0.1 mg l-1. The relative standard deviations for the analysis of 2-phenylethanol were in the range of 1.5-2.4%. The relative recoveries of 2-phenylethanol at spiking levels of 10, 75 and 150 mg l-1 were 93.7, 96.9 and 97.2%, respectively. The enrichment factor of the proposed method was 123. The proposed method is a simple, fast, accurate, highly stable and
In this paper, deep eutectic solvents (DESs) were investigated as a new kind of dispersive solvent for effective dispersive liquid-liquid microextraction (DLLME) for the first time. It was established that the DESs can increase the extraction efficiency compared to conventional polar, water-miscible organic dispers
In this paper, a dispersive liquid-liquid microextraction method has been developed for the extraction and preconcentration of natamycin from cheese and doogh samples and its determination by...
Determination of chlorophenols in water using dispersive liquidliquid microextraction coupled with water-in-oil microemulsion electrokinetic chromatography in normal stacking mode Journal of Separation Science, 2017, 40 , 26622670 Ludi Shi, Jin Wang, Jing Feng, Sihan Zhao, Zhengmeng Wang, Hu Tao, Shuhui Liu Abstract The current routes to couple...
Dispersive liquid-liquid microextraction (DLLME) is a ternary extraction system that consists of an extraction solvent, dispersing solvent and an aqueous sample containing the analyte of interest. DLLME is a powerful, miniaturised extraction technique that can increase an analytes concentration and achieve high enrichment factors (EFs) which makes it ideal for trace analysis. DLLME consists of injecting the binary mixture of extraction/dispersing solvent into the sample. This creates a very large surface area of fine extraction droplets. It is these droplets that analytes enrich, almost instantaneously into. Given the speed of the extraction, DLLME is a very attractive procedure that can increase sample throughput whilst lowering solvent consumption, waste and associated costs. Several organic acids were identified as common constituents in milk and dairy produce. Organic acids appear in milk and dairy produce due to natural biochemical processes within the animal, bacterial activity, as ...
Zhang, H., & Shi, Y. (2010). Temperature-assisted ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography for the determination of anthraquinones in Radix et Rhizoma Rhei samples. Talanta, 82(3), 1010-1 ...
A bead-based sedimentation biodetector is studied theoretically. The biodetector operates with a suspension of settling beads, non-settling reporters, and target analytes - all initially suspended in a buffer solution. The reporters can be either fluorescent molecules or small particles. The functionalized beads interact with the reporters and target analytes while settling under the action of gravitational, electric, and/or magnetic fields. Both sandwich and competitive assays with hindered settling are considered. In the sandwich format, in the presence of target analytes, the reporters bind to the beads and settle (the target analytes provide the link between the beads and the reporters). A reduction in the reporters concentration indicates the presence of target analytes. In the competitive format, both target analytes and reporters compete for bead-based binding sites. In the absence of target analytes, one would observe a reduction in the suspended reporters concentration. The model allows one
Two extraction strategies for albendazole, chloramphenicol, trimethoprim, enrofloxacin, oxitetracycline and nicarbazin in egg were optimized for its quantitation by liquid chromatography. First, two designs were built to find out the optimized condition for the air assisted-dispersive liquid-liquid microextraction based on solidification of organic drop: a fractional factorial and a central compos ...
Two extraction strategies for albendazole, chloramphenicol, trimethoprim, enrofloxacin, oxitetracycline and nicarbazin in egg were optimized for its quantitation by liquid chromatography. First, two designs were built to find out the optimized condition for the air assisted-dispersive liquid-liquid microextraction based on solidification of organic drop: a fractional factorial and a central compos ...
The present invention is directed to methods and compositions useful as biosensors that specifically interact with various pathogens and other target analytes. The biosensor itself, comprises functionalized dendritic tethers derivatized for attachment to a variety of surfaces as self-assembled monolayers (SAMs) as well as attached binding moieties (sometimes referred to as capture binding ligands). Accordingly, the present invention provides compositions comprising supports comprising surfaces to which the binding moieties (e.g. antibodies) are attached for the detection of target analytes (e.g. pathogens) as well as methods and compositions relating to the attachment of such binding moieties.
A patients health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patients health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable
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The extraction yield of a microextraction technique depends on thermodynamic and kinetics factors. Both of these factors have been the focus of intensive research in the last few years. The extraction yield can be increased by synthesizing and using novel materials with favorable distribution constants (one of the thermodynamic factors) for target analytes. The extraction yield can also be increased by improving kinetic factors, for example, by developing new extraction modes. Microextraction techniques are usually non-exhaustive processes that work under the kinetic range. In such conditions, the improvement of the extraction kinetics necessarily improves the performance. Since the extraction yield and efficiency is related to how fast the analytes diffuse in samples, it is crucial to stir the sample during extraction. The stirring can be done with an external element or can be integrated with the extraction element in the same device. This article reviews the main recent advances in the so-called
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HPLC Part: AJ0-8783 SecurityGuard™ ULTRA cartridges for WIDEPORE C18 UHPLC, sub-2µm and core-shell columns with 2.1mm internal diameters (ID), 3/Pk Recomended Use: Column protection for UHPLC columns Separation Mode: Reversed Phase Format: Guard Cartridge (e.g. PHE SecurityGuard )
The existing conceptions on the problem of liquid phase sintering fail to give answers to many questions like the following: why do compacts during liquid-phase sintering grow at first and then...
This application note demonstrates a 2D-IC-MS system that allows low levels of small organic acids (C1-5) to be analyzed in a high-ionic-strength matrix. The first dimension of the system separates the low-level analytes from the matrix while the second dimension resolves each of the target analytes. The mass spectrometer confirms identity and achieves low detection limits for a sensitive and selective assay.
SPE Part: 8B-S016-RCH Strata® Screen-C (55 µm, 70 Å), 300 mg / 6 mL, Tubes , 30/Pk Phase: Mixed Mode C8 and SCX Sorbent Type: Silica-based Format: Tube Target Analytes: Basic drugs from biological matrices
An arrangement for chemical analysis of a small quantity of sample wherein a specimen of a small size is passed through a porous distribution first medium onto a reagent-containing second medium is disclosed. The reagent-containing second medium is a thin, flat, liquid-impervious medium. A reagent is encapsulated upon the second medium as a flat, liquid-phase surface. The first and second mediums are so arranged and disposed that when firmly pressed together, the encapsulated liquid reagent will be liberated and the specimen will be distributed through the first medium onto the liquid-phase liberated reagent where the subsequent reaction of the liquid-phase reagent and the specimen can then be identified by reading means.
An arrangement for chemical analysis of a small quantity of sample wherein a specimen of a small size is passed through a porous distribution first medium onto a reagent-containing second medium is disclosed. The reagent-containing second medium is a thin, flat, liquid-impervious medium. A reagent is encapsulated upon the second medium as a flat, liquid-phase surface. The first and second mediums are so arranged and disposed that when firmly pressed together, the encapsulated liquid reagent will be liberated and the specimen will be distributed through the first medium onto the liquid-phase liberated reagent where the subsequent reaction of the liquid-phase reagent and the specimen can then be identified by reading means.
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The stability of twenty seven composts and organic substrates (including raw, less stable and stable materials) was assessed using two different liquid phase tests were carried out. One of the tests was introduced in 1998 and was based on the calculation of a Specific Oxygen Uptake Rate (SOUR). The newly introduced liquid phase test presented here is simpler to set-up and to perform that the older liquid phase test. It is based on the quantification of oxygen consumption in the headspace of a BOD bottle that contains the liquid-solid solution. The results indicate that a marginal correlation does exist between the main indices calculated from both tests. The correlation was stronger for the indices calculated for stable-processed organics than for the raw (unprocessed materials). The SOUR ranged from 1520 to 3650mg O2/kg VS-h for the raw materials and from 110 to 1150 mg O2/kg VS-h for the processed materials, respectively. The corresponding stability rate related index (LSRI24) of the new liquid phase
Biomass has the potential to serve as a sustainable source of energy and organic carbon for our industrialized society. The focus of this Review is to present an overview of chemical catalytic transformations of biomass-derived oxygenated feedstocks (primarily sugars and sugar-alcohols) in the liqui …
This paper proposes a variation of the incremental approach to identify reaction and mass-transfer kinetics (rate expressions and the corresponding rate parameters) from concentration measurements for both homogeneous and gas-liquid reaction systems. This incremental approach proceeds in two steps: (i) computation of the extents of reaction and mass transfer from concentration measurements without explicit knowledge of the reaction and mass-transfer rate expressions, and (ii) estimation of the rate parameters for each rate expression individually from the computed extents using the integral method. The novelty consists in using extents that are computed from measured concentrations. For the computation of the individual extents, two cases are considered: if the concentrations of all the liquid-phase species can be measured, a linear transformation is used; otherwise, if the concentrations of only a subset of the liquid-phase species are available, an approach that uses flowrate and possibly gas
Over 200 combinations of column configurations and chemistries, UPLC Columns give faster separations with higher resolution and sensitivities using sub 2 µm particles at 1241 bar pressures
Med Agilent AdvanceBio peptidmappningskolonner kan du snabbt separera och identifiera aminosyra-modifikationer i primärstrukturen. Dessa avancerade biokolonner har en porstorlek på 120Å med solid core 2,7µm partiklar. De är speciellt testade med en utmanande peptidstandard för att säkerställa tillförlitlig peptidmappning. Dessutom levererar AdvanceBio peptidmappningskolonner exceptionell upplösning och hastighet för UHPLC och konventionell HPLC.
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A switchable solvent-based liquid phase microextraction (SS-LPME) procedure has been established for the preconcentration of copper prior to its determination by microsampling flame atomic absorption spectrometry (FAAS). Triethylamine (TEA) was adopted for the study as a green and cheap switchable solvent. The Cu(II)-1-(2-pyridylazo)-2-naphthol (PAN) complex was extracted into the TEA phase by converting the protonated carbonate (P-TEA-C) to TEA. The experimental conditions have been comprehensively studied and optimized. The limit of detection (LOD) and repeatability (RSD%) of the method were 1.80 mu g L-1 and 3.8%, respectively, and its accuracy was assessed against certified reference materials (TMDA-64.2 fortified water, TMDA-53.3 fortified water, TMDA-51.3 fortified water, 1573a tomato leaves, INCT-OBTL-5 oriental basma tobacco leaves and NCS ZC 8100 2b human hair), and by addition-recovery tests. The SS-LPME-FAAS method was also applied for the determination of trace amounts of copper in ...
A method and apparatus for automatically performing liquid microextraction on liquid samples includes the steps of controlling movement of a syringe between a cleaning station, a sample station containing a plurality of discrete sample vials and an instrument injector station. Movement of the syringe is controlled automatically for cleaning the syringe, obtaining a sample of each sample in each discrete sample vial, one at a time, and injecting the collected sample into the instrument injector, and then repeating the sequence steps for all discreet samples.
In this paper, a new method of annular platform-supported headspace liquid-phase microextraction (LPME) was designed using ionic liquid as an extraction solvent, wherein extraction stability and efficiency were improved by adding an annular platform inside the extraction bottle. The ionic liquid 1-silicyl-3-benzylimidazolehexafluorophosphate was first synthesized and proved to be an excellent extraction solvent. Coupled with liquid chromatography, the proposed method was employed to analysis of polycyclic aromatic hydrocarbons (PAHs) in water and optimized in aspects of extraction temperature, extraction solvent volume, extraction time, pH, stirring rate, and salt effect of solution. The results indicated that this method showed good linearity (|i|R|/i||sup|2|/sup| > 0.995) within 0.5 |i|µ|/i|g·L|sup|−1|/sup| to 1000 |i|µ|/i|g·L|sup|−1|/sup| for PAHs. The method was more suitable for extraction of volatile PAHs, with recoveries from 65.0
By using ionic liquid as membrane liquid and tri-n-octylphosphine oxide (TOPO) as additive, hollow fiber supported liquid phase microextraction (HF-LPME) was developed for the determination of five sulfonamides in environmental water samples by high-performance liquid chromatography with ultraviolet detection The extraction solvent and the parameters affecting the extraction enrichment factor such as the type and amount of carrier, pH and volume ratio of donor phase and acceptor phase, extraction time, salt-out effect and matrix effect were optimized. Under the optimal extraction conditions (organic liquid membrane phase: [C8MIM][PF6] with 14% TOPO (w/v); donor phase: 4mL, pH 4.5 KH2PO4 with 2M Na2SO4; acceptor phase: 25microL, pH 13 NaOH; extraction time: 8 h), low detection limits (0.1-0.4microg/L, RSD,or=5%) and good linear range (1-2000ng/mL, R(2),or=0.999) were obtained for all the analytes. The presence of humic acid (0-25mg/L dissolved organic carbon) and bovine serum albumin (0-100 ...
0049]The diesel boiling range distillate and hydrogen in line 44 is fed once-through to the liquid-phase continuous reaction zone 16 and to first liquid-phase continuous reactor 52 and/or second liquid-phase continuous reactor 56. No effluent from the liquid-phase reaction zone 16 which may comprise ultra low sulfur diesel is recycled back to the reaction zone 16. No effluent from the first liquid-phase continuous reactor 52 which may comprise ultra low sulfur diesel is recycled back to the first liquid-phase continuous reactor 52. No effluent from the second liquid-phase continuous reactor 56 which may comprise ultra low sulfur diesel is recycled back to the second liquid-phase continuous reactor 56. The diesel boiling range distillate fed to the first liquid-phase reactor 52 is absent liquid-phase reaction zone diesel effluent and/or hydrogen recycled from the first liquid-phase reactor 52. The diesel boiling range distillate fed to the second liquid-phase reactor 56 is absent liquid-phase ...
Aifeimu Chemical Co., Ltd. produces Atorvastatin Calcium/Fluvastatin Sodium/Rosuvastatin Calcium/Pitavastatin Calcium/ezetimibe/Atorvastatin Calcium
In recent years, advances in direct sample to mass spectrometry (MS) techniques have allowed for the application of these methods towards quantitative analysis in complex matrices such as biofluids and tissue. However, the predictable limitations of these technologies, such as ionization suppression, poor sensitivity at trace levels, and narrow linear dynamic range, have been the driving force toward the development of methods that efficiently integrate sampling, sample cleanup, and analyte collection and ionization. In this context, the direct interface of microextraction technologies and MS has undoubtedly revolutionized the speed, efficacy, and robustness with which complex matrices can be scrutinized. In this thesis, numerous strategies recently developed for the direct and efficient coupling of Solid Phase Micro Extraction (SPME) and MS are presented towards the analysis of complex matrices. Aiming to supply a range of technologies suited for diverse applications, different SPME geometries ...
These perfectly tender fresh orange scones have such a bright citrus flavor and are topped off with a sweet + tangy orange glaze.
These perfectly tender fresh orange scones have such a bright citrus flavor and are topped off with a sweet + tangy orange glaze.
Ionic liquid gel polymer electrolytes (ILPEs) based on electrospun P(VdF-HFP) membrane were prepared by incorporating RTILs, BMImTESI or BMImBF(4). A suitable BMIm cation based ionic liquid with lithium salt and influence of temperature on electrochemical performance was investigated. The BMImTFSI-based polymer electrolyte with LiTFSI salt showed better electrochemical properties and the properties increase with increasing the operation temperature to 40 degrees C, to 140 mAh g(-1) discharge capacity at 1 C-rate. However, after 40 degrees C the properties remained the same even when the temperature was increased at high current density.
Alcamizer offers manufacturers high heat stability along with high transparency but without deteriorating the insulating characteristics of PVC. It works by trapping hydrochloric acid in the PVC by an ion exchange reaction. Alcamizer is also environmentally safe, as it contains no heavy metals or any sulphides.. "Alcamizer is a safe and flexible heat stabilizer and we are very pleased to make it available in our distribution channels" said Mr. Hamid Amiri, APTs Managing Director. "As Kisuma is a market leader and global supplier of magnesium hydroxide and synthetic hydrotalcites for plastics applications, its products fit well into the sectors targeted by APT.". Kisuma has a range of compounds that are used mainly in the engineering plastics and polymer processing industries. Along with its PVC heat stabilisation products, Kisuma compounds can be used for adding flame retardancy to cables and for halogen scavenging in poly propylene and other bulk polymers.. About Kisuma Chemicals BV ...
Make sure you rinse the beans really really good too…Oh and if youre not a lemon fan try orange juice or leave out the zest. I just made another batch with fresh orange juice and my hubby liked them better - I prefer the lemon..Glad you enjoyed :). ...
Vitamin C is an everyday essential. Whether you enjoy a glass of fresh orange juice in the morning, or a helping of broccoli with your evening meal, you need to squeeze enough Vitamin C into each day. From protecting our cells against damaging free radicals, to helping produce antibodies, this nutrient can do your bo
Fresh orange juice and cinnamon infuse this winter squash and chicken stew. Tzimmes (pronounced tsim-iss) can also be made with brisket and is often served during the Jewish New Year. A fairly sweet dish, its said to offer wishes for a sweet year ahead.
Aside from the more well-known tips like washing your hands and gulping down a glass of fresh orange juice, here are 9 ways to boost your immune system.
Iron tablets can sometimes make you go more often so dont discount that possiblity! Either way a bit grim, if you take them with fresh orange juice they absorb better too (or any form of vitamin C...at least that is what I was told to do). If you do have the camera up your nether regions maybe you should overcome the embarrassment and get it out there(so to speak!!) People are always embarrassed by nether regions and talking about them but they are parts of the body like anything else ...
For demanding applications when you need to collect more information in less time, our Flexar UHPLC is the perfect solution. This UHPLC is designed for a high productivity environment and delivers high sensitivity and resolution, exceptional flow accuracy and precision and faster results.
A highly efficient, simple and rapid method, injection-ultrasound-assisted emulsification microextraction based on using low-density organic solvents followed by high-performance liquid chromatography-ultraviolet detection, was developed for the dete
This invention is directed to systems and methods for removing lipids from a fluid, such as plasma, or from lipid-containing organisms. These systems contact a fluid with an extraction solvent, which causes the lipids in the fluid to separate from the fluid or causes lipids in the lipid-containing organisms to separate from the lipid-containing organism, using at least one hollow fiber contactor. The separated lipids are removed from the fluid. The extraction solvent is removed from the fluid or at least reduced to a level below a particular threshold enabling the fluid to be administered to a patient without the patient experiencing undesirable consequences. Once the fluid has been processed, the fluid may be administered to a patient who donated the fluid, to a different patient, or be stored.
Nowadays in different analytical laboratories there is the increasing number of analytical protocols, either based on highly sophisticated or simpler measurements techniques, which serving for determination of different target analytes of pharmacological importance. Among such target groups of the analyte belongs the antibiotics which present a great discovery in the field of medicine and thanks to them were saved more than seven million people but beside to the mentioned great benefits, antibiotics can cause a large number of side effects and bile acids together with their derivatives which are physiological detergents but if their concentration is not controlled they can be cytotoxic to the body. In the present doctoral dissertation the development of analytical methods, primarily analytical voltammetric methods in combination with simple and contemporary electrodes/sensors, for the determination of selected analytes as antracycline antibiotic doxorubicin (DOX), macrolide antibiotics ...
Flow cytometry and mass spectrometry are widely used analytical tools in cancer research. Flow cytometry is known for cell counting, cell sorting and cancer biomarker discovery. Mass spectrometry is unparalleled in its ability to selectively detect and quantify target analytes at the molecular level. The combination of flow cytometry to isolate specific cell subtypes from biological fluids such as blood and mass spectrometry to quantify proteins contained in the selected cells provides a new approach to studying protein expression in specific cell subtypes. We have used this combination to investigate the normal levels of CD47 and SIRPA proteins in CB8+ T-cells, CD4+ T-cells, CD14+ monocytes, CD33+ myeloid cells and CD56+ NK cells isolated from blood specimens as a first step toward better understanding of how the CD47/SIRPA protein levels in these cells are affected by cancer and cancer treatment.. Citation Format: Carmen Fernandez-Metzler, Renold Capocasale. LC-MS/MS quantification of proteins ...
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