Article: We report studies of the interaction of Alzheimers amyloid beta protein (Aβ) with normal human plasma high density lipoprotein (HDL), aiming to clarify to which lipoprotein (LP) structural constituent (apolipoprotein or lipid) soluble Aβ is primarily bound. Purified HDLs were incubated wit...
Understanding the determinants of HDL function is widely considered a critical priority in HDL research. Given the heterogeneity of HDL species in the circulation, understanding the transporters essential for cholesterol efflux and the HDL species, which are the major contributors to this process may direct contemporary strategies to optimize HDL function or deliver novel HDL analogues in vivo. The present study, which represents the most detailed evaluation of HDL particle size and cellular cholesterol efflux via ABCA1 and ABCG1 to date, has identified HDL size as a key determinant of the efflux capacity of HDL. We further demonstrate that ABCA1 is a quantitatively critical mediator of cholesterol efflux to HDL3, and that HDL3b, HDL3c and lipid-free apoA-I are all suitable therapeutic targets for optimizing cellular cholesterol efflux.. It is a widely held view that ABCA1 is an important mediator of cholesterol export and that it specifically requires apolipoproteins (such as apoA-I) that ...
1. There are two distinct binding sites (Site 1 and Site 2) for human low-density lipoprotein (LDL) on rat hepatocytes in monolayer culture [Salter, Saxton & Brindley (1986) Biochem. J. 240, 549-557]. 2. Binding of 125I-LDL to Site 1, but not to Site 2, is up-regulated between 20 and 44 h in culture by preincubation of the cells with human high-density lipoprotein 3 (HDL3). 3. A similar preincubation with HDL2 had no significant effect on binding to either site. 4. Preincubation with human LDL led to a partial down-regulation of subsequent binding of 125I-LDL to Site 1. Since binding after incubation with LDL was measured at 37 degrees C, binding to Site 2 could not be distinguished from LDL that had been internalized by the cells. 5. Hepatocytes were shown to degrade 125I-LDL, resulting in the accumulation of [125I]iodotyrosine in the medium. Evidence was found that iodotyrosine may be further degraded by deiodinase produced by the cells. 6. Regulation of binding to Site 1 by preincubation with ...
In this study, we 1) developed a new native PAGE system which quantitatively separates a smaller HDL particles from other HDL particles; 2) demonstrated that this HDL fraction is preβ1-HDL, which can be converted to other HDLs by LCAT; 3) found that preβ1-HDL particles thus isolated are spheroidal with the highest apoA-I/cholesterol ratio and highest density (≥1.21 g/ml), compared with all other HDL particles; 4) found that neonates have significantly higher and more complex preβ-HDLs than adults; 5) found that Tangier patients and CETP mutation have no detectable preβ-HDLs; 6) found that the existing two-dimensional gel system cannot precisely quantify preβ1-HDL in human serum; and 7) found that preβ1-HDL is significantly related to the severity of coronary artery stenosis in a negative manner.. One of the key accomplishments of this study is that we established a simple and fast method for preβ1-HDL quantification. It has been reported that HDL subclasses can be defined by either of ...
TY - JOUR. T1 - Effects of diet, physical fitness and hormones on high density lipoprotein (part I. T2 - Diet). AU - Lyu, Li-Ching. AU - Yi Chun Lai, Chun Lai. AU - Wu, W. H.. PY - 1999/12/1. Y1 - 1999/12/1. N2 - High density lipoprotein (HDL) plays the central role n reverse cholesterol transport, which with highest density and abundant protein component of the lipoprotein moieties. Nascent HDL accepts unesterified cholesterol from peripheral tissues, esterified the free cholesterol and moves cholesterol ester to the core of HDL, then carries the cholesterol ester to liver for further metabolism. Therefore, HDL decreases the peripheral cholesterol deposition and reduces the risk for developing atherosclerotic diseases. In 1988 and 1993, both versions of the National Cholesterol Education Program in U.S.A. added HDL-Cholesterol (HDL-C) less than 35 mg/dL as a risk for coronary heart disease along with other risk factors such as diabetes and hypertension. The nutritionists in Taiwan often were ...
High Density Lipoprotein is something that is very essential in the body to prevent diseases. there are various sources of this substance. Read this article to learn of the foods that contain the High Density Lipoprotein.
Fingerprint Dive into the research topics of High density lipoprotein metabolism in endurance athletes and sedentary men. Together they form a unique fingerprint. ...
The present study is the first study to show that higher cholesterol levels in small HDL and medium HDL particles were associated with lower risk of total stroke, either ischemic or hemorrhagic stroke even after adjustment for known cardiovascular risk factors and matching variables of age, sex, years of serum storage, fasting status, and community. There was no association between L-HDL cholesterol levels and risk of total stroke and its subtypes. Risk of total stroke was ≈90% lower among persons at the highest quartile of S-HDL cholesterol levels or M-HDL cholesterol levels than among those at the lowest quartile. S-HDL and M-HDL cholesterol levels were not associated with age, sex, blood pressure levels, body mass index, smoking, and diabetes mellitus. Taken together, higher cholesterol levels in S-HDL and M-HDL are suggested to reduce risk of stroke beyond the effects of other conventional risk factors. Small HDL and medium HDL cholesterol levels can be increased by increasing dietary ...
Vessel Plus is an open acccess journal, which publishes articles related to vascular diseases, including acute respiratory distress syndrome, aneurysm, atherosclerosis, hypertension, stroke, peripheral vascular or pulmonary vascular diseases, etc.
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The link between HDL and cardiovascular disease (CVD) is substantially more complex than ) originally thought. Until recently, the concentration of HDL was deemed sufficient in predicting future cardiovascular events. However, HDL function can become compromised in various disease states, resulting in reduced cardio-protection and the production of dysfunctional HDL with reduced anti-atherogenic properties. Antioxidants have been implicated in ameliorating the damaging oxidative processes associated with dysfunctional HDL and atherosclerosis. Therefore, this thesis focuses on the evolving role of HDL function and had three main aims: i) to assess the complex relationship between HDL-cholesterol concentration and HDL function, ii) to assess the modulation of HDL function by the ) predominant form of vitamin E, α-tocopherol, encompassing in vitro and ex vivo investigations and iii) to assess the modulation of HDL function by lycopene by dietary interventions. Various HDL-associated enzymes, ...
Abstract: An ability of high density lipoproteins HDL3 to accept cholesterol from erythrocyte membranes was studied in healthy persons and in patients with ischemic disease of heart. The cholesterol-acceptory function was estimated as follows: by incorporation of fluorescent probes (cholestatriene and pyrene) into particles of HDL3, by elimination of cholesterol from erythrocyte membranes and by increase of the lipoproteins size evaluated using the method of quazi-resilient dispersion of laser light. In ischemic disease of heart the property of high density lipoproteins, specifically of HDL3 fraction, to accept cholesterol from cell membranes was impaired. Middle size of HDL3 particles was decreased in the patients with ischemic heart disease as compared with that of healthy persons ...
Lipoprotein, illustration. This is a high density lipoprotein (HDL), or good cholesterol, molecule. It consists of a core of esterified cholesterol molecules surrounded by a shell of unesterified cholesterol (orange with violet cap) and phospholipids (orange with blue cap). The complex structure includes carrier proteins (green) known as apolipoproteins, which assist transport in the blood. HDL cholesterol plays a role in fat metabolism and contributes to cardiovascular health. - Stock Image F012/8897
Background: Exercised-based cardiac rehabilitation (CR) can increase HDL-cholesterol (HDL-C). However it remains unclear how elevated HDL-C and changes of HDL subfractions are correlated with the improvement of exercise tolerance in acute coronary syndrome (ACS) patients participated with CR.. Methods: Concentrations of cholesterol and apolipoproteins (Apo) in HDL subfractions separated by heparin-Mn precipitation method were measured at the onset of ACS and at the end of the 6-month CR program in 51 patients (45 men and 6 women) aged of 64.3 ± 11.8 years. All patients received successful percutaneous coronary intervention on admission, and then started to take statins.. Results: Serum levels of HDL-C and ApoA1, and concentrations of cholesterol and apoA1 in large HDL fraction (HDL2) were significantly increased by CR (42.7 mg/dl ± 14.1 mg/dl to 47.4 mg/dl ± 14.2 mg/dl, 128.8 mg/dl ± 23.4 mg/dl to 139.2 mg/dl ± 26.0 mg/dl, 25.3 mg/dl ± 12.4 mg/dl to 30.3 mg/dl ± 13.6 mg/dl, 67.8 mg/dl ± ...
High Density Lipoproteins (HDL) Interrupt the Sphingosine Kinase Signaling Pathway: A possible Mechanism for Protection Against Atherosclerosis by ...
In order to assess the role of HDL on longevity, we studied HDL subfraction distribution in centenarian women compared with a group of weight- and gender-matched healthy normolipidemic controls. We did not find any significant difference in the mean plasma lipid, apolipoprotein, and Lp(a) levels. On the contrary, in spite of similar HDL-cholesterol concentrations (1.32 +/- 0.41 mmol/l in centenarians vs. 1.32 +/- 0.25 mmol/l in controls, p = not significant), HDL2b and HDL3a levels were, respectively, significantly increased and significantly reduced in centenarians in comparison with controls (HDL2b 32.4 +/- 9.2% in centenarians vs. 23.4 +/- 7.7% in controls, p less than 0.002) and HDL3a 26.3 +/- 9.8% in centenarians vs. 34.1 +/- 7.3% in controls, p less than 0.01). HDL2b levels were significantly raised and HDL3a levels were significantly reduced in centenarians in comparison with both middle-aged and elderly subjects, whereas no difference for any HDL subfraction was found between the two ...
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It has been established that the level of plasma high-density lipoprotein (HDL)-cholesterol is inversely proportional to the risk of coronary heart disease (CHD). HDL particles are highly heterogeneous, particularly in terms of their biological activities, many of which are atheroprotective. For example, in addition to carrying out reverse cholesterol transport, HDL protects the vascular endothelium by inhibiting monocyte adhesion and the oxidative modification of low-density lipoprotein (LDL), eliminates some of the atherogenic products of LDL oxidation, and possesses antithrombotic activity, due to the inhibition of platelet activation and aggregation. Statin therapy has been shown to increase the level of plasma HDL-cholesterol. However, the clinical benefit of statin-induced elevation of HDL-cholesterol is unclear from trial data, perhaps as a result of the concomitant overwhelming risk benefit of statin-induced reduction of LDL-cholesterol.. ...
High-density lipoprotein (HDL) has previously been shown to promote angiogenesis. However, the mechanisms by which HDL enhances the formation of blood vessels remain to be defined. To address this,...
Triglyceride, high density lipoprotein, and coronary heart disease by Naomi Miller; 1 edition; Subjects: Bibliography, High density lipoproteins, Triglycerides, Coronary heart disease
Statin drugs are credited with a significant reduction of cardiovascular disease over the past 20 years. However, even when LDL (low-density lipoprotein cholesterol) has been properly reduced, heart attacks still occur. Additional targets besides LDL-lowering must be explored, such as HDL.. HDL (high-density lipoprotein cholesterol), when elevated, is associated with a lower incidence of heart attacks, strokes and death. HDL protects the heart. Low levels of HDL are associated with higher risk of heart disease and mortality. HDL promotes the removal of excess lipid material from the arteries. Low HDL can be due to smoking and to genetic disease. Statin drugs cannot compensate for the risk resulting from low HDL. Low HDL levels help define metabolic syndrome.. The minimum level of HDL thought to be heart protective is 35 to 40 mg/dL. The desirable level for men is 40 mg/dL and for women is 50 mg/dL. Thirty-five percent of men are below 40 and 39% of women are below 50.. Lipoproteins, made of fats ...
Dear Reader,. A heart-y congratulations is in order for your commitment to regular physical activity. Its also great that youre keen to get to the bottom of your blood test results. All the talk about "good" and "bad" cholesterol, can make figuring out your cholesterol numbers a little confusing. First, the basics - cholesterol is made up of low-density lipoproteins (LDL) and high-density lipoproteins (HDL). Youre correct in your knowledge about the difference between HDL and LDL - HDL is the type of cholesterol that is beneficial for the body because it carts away cholesterol to the liver, which then removes it from the body. Generally speaking, a higher HDL level is recommended and an HDL level between 40 to 50 milligrams per deciliter (mg/dL) is good (while a level higher than 60 mg/dL is even better). So, when it comes to the "good" stuff, youre almost there! On the flipside, a lower level of LDL is advised, but youre right on track because an LDL level of 124 mg/dL falls into the ...
Developing new pharmacotherapies for cardiovascular disease is of paramount importance but a time-consuming, costly endeavor. Agents that increase high-density lipoprotein levels appeared initially attractive, but recent experience with the cholesterylesterase transfer protein (CETP) inhibitor torcetrapib was associated with increased mortality attributed to off-target effects. Dalcetrapib, a new CETP inhibitor, was tested in the phase 2b, randomized, double-blind, placebo-controlled dal-PLAQUE (A Study of the Effect of Dalcetrapib on Atherosclerotic Plaque in Patients with Coronary Heart Disease) trial that included atheroma characterization endpoints by serial carotid MRI and FDG PET imaging (12). At 6 months, dalcetrapib increased high-density lipoprotein levels by 34%. PET imaging demonstrated a modest nonsignificant trend towards decreased carotid FDG activity compared with controls (−7.3% TBR reduction, 90% confidence interval: −13.5 to −0.8). Serial MRI, however, showed a relative ...
Individuals with low levels of high-density lipoprotein (HDL; "good") cholesterol and heart disease may benefit from treatment targeted at raising HDL levels. For the 30-month study, 143 retired military personnel with heart disease and low HDL were randomly assigned to a placebo treatment or aggressive HDL-cholesterol-raising therapy with gemfibrozil, niacin, and cholestyramine. The participants also had diet and exercise counseling. The studys findings showed that the participants in the active treatment group had a 20% decrease in total cholesterol, a 36% rise in HDL cholesterol, a 26% reduction in low-density lipoprotein (LDL; "bad") cholesterol, and a 50% fall in triglycerides, compared with the placebo group. Improvement in the narrowing of the coronary arteries increased by 0.8% in the individuals on active therapy, compared with a decrease of 1.4% in the placebo group. The researchers also determined that significantly more individuals on the placebo therapy than active therapy (26% vs ...
Results Pre-incubation with rHDL inhibited the hypoxia-induced increases in PHD2 and PHD3 levels (32% and 45% respectively, p,0.05) while Siah1 and Siah2 mRNA levels were increased (58% and 88% respectively, p,0.05) with rHDL pre-incubation. Following siRNA knockdown of Siah1 and Siah2, HDL lost its ability to induce the expression of HIF-1α (11% reduction, p,0.05) and VEGF (18% reduction, p,0.05). Augmentation of tubulogenesis by HDL was attenuated in Siah siRNA-transfected cells (55% in siSiah1; 40% in siSiah2, p,0.05). Siah knockdown also abrogated HDL inhibition of hypoxia-mediated induction of PHD2 and PHD3 (p,0.05). PI3K and Akt are upstream regulators of HIF-1α/VEGF and also regulate Siahs. Specific inhibition of the PI3K/Akt pathway revealed that it plays a key role in HDL-induced elevations in Siah1 and Siah2 (37% and 20% reduction respectively, p,0.05) as well as HIF-1α and VEGF (65% and 15% reduction respectively, p,0.05). ...
Background: In population studies high HDL is associated with fewer CHD events, however in certain people high HDL does not appear to protect and recent work suggests that HDL can become dysfunctional in settings of inflammation. Since Metabolic Syndrome (MetSyn) patients often have systemic inflammation, we hypothesized that high HDL might not be protective in this group.. Methods: We analyzed data from 6,814 subjects in MESA; 2,362 of whom had MetSyn. Subjects were stratified by HDL into low (, 40), mid (40 -59) and high (≥ 60 mg/dL) groups. Outcomes were cIMT, coronary calcium (CAC), and CHD events over 3 years of follow up. Cox proportional hazards models assessed the association of HDL with CHD events.. Results: In non-MetSyn subjects, outcomes were worst in the low HDL group and improved as HDL increased. In MetSyn subjects, however, an unexpected U-shaped relationship was seen. The most favorable results were in MetSyn subjects with mid HDL (40 -59), while worse outcomes were seen with ...
Question - Have normal HDL, LDL, cholesterol, blood pressure. Found raised CRP level. What does this indicate?. Ask a Doctor about diagnosis, treatment and medication for Joint pains, Ask a Radiologist
A customer recently asked me what he could do to raise his low HDL "good cholesterol" levels. HDL stands for High Density Lipoprotein and is not a type of cholesterol, but a carrier of it. A low ratio of HDL / Total Cholesterol of less than 10 is a strong risk factor for cardiovascular disease (over 24 is ideal ...
HDL stands for high-density lipoprotein and is used to describe one of the two types of cholesterol in the body. HDL cholesterol is known as the good cholesterol, while low-density lipoprotein...
A quick, 30-minute walk each day is all you need to increase high-density lipoprotein (HDL) or good cholesterol by 9 percent. Cant squeeze in a dai
Northwestern University researchers said they have discovered a nanoparticle that could be the basis for a nontoxic way to eliminate cancerous lymphoma from the body.. The nanoparticle, which the researchers tested in cell cultures and on mice, fools the lymphoma cells into thinking it contains high-density lipoprotein (HDL), which the cells need to survive.. The nanoparticle has a similar shape, chemistry and size as high-density lipoprotein, but it has a tiny gold particle at its core. The function of high-density lipoprotein is to carry cholesterol in the bloodstream.. All of the cells in the human body need cholesterol to survive, said Dr. C. Shad Thaxton, an... ...
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Genetically modified tomatoes created at UCLA produce 6F peptide that mimics the action of apoA-1, the chief protein in high-density lipoprotein (HDL).
Tangier disease is a rare inherited disorder characterized by a severe reduction in the amount of high-density lipoprotein (HDL).
Digital hardware design typically is done using a specialized language, called a hardware description language (HDL). This approach is based on the idea that hardware design has unique requirements. The mainstream HDLs are Verilog and VHDL.
Change time conversion and treat single element BAT differently than normal BATs for print width computation (for backwards compatibility reasons). diffs (193 lines): diff --git a/clients/mapilib/mapi.c b/clients/mapilib/mapi.c --- a/clients/mapilib/mapi.c +++ b/clients/mapilib/mapi.c @@ -4145,7 +4145,7 @@ static char* mapi_convert_unknown(struct static MapiMsg -read_into_cache_new(MapiHdl hdl, int lookahead, int expect_result, int *error) +read_into_cache(MapiHdl hdl, int lookahead) { char *line, *copy; Mapi mid; @@ -4163,7 +4163,6 @@ read_into_cache_new(MapiHdl hdl, int loo if (result && result-,next && result-,next-,prot10_resultset) { // if we have a prot10 resultset we dont want to read lines, because prot10 result sets do not consist of lines hdl-,active = result-,next; - if (expect_result) expected_prot10_result(error); return MOK; } for (;;) { @@ -4177,7 +4176,6 @@ read_into_cache_new(MapiHdl hdl, int loo lng nr_rows; lng nr_cols; lng i; - expect_result = 0; result = new_result(hdl); ...
Every 1% increase in HDL is associated with a 1%???3% reduction in heart attack risk. So if your LDL is already down, keep after your doctor to focus on your…
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TY - JOUR. T1 - The efficacy of gemfibrozil therapy for raising high density lipoprotein levels. AU - Weis, Stephen. AU - Kudchodkar, B. J.. AU - Clearfield, M. B.. AU - Lacko, Andras G.. PY - 1992/1/1. Y1 - 1992/1/1. N2 - Thirty subjects, 5 normotriglyceridemic (NTG) with low HDL cholesterol (HDL-C , 35 mg/dl) and 25 hypertriglyceridemic (HTG) with low and high HDL-C (HDL-C , 35 mg/dl) were selected for this study. They were treated with gemfibrozil (600 mg BID) for 12 weeks. In both groups, gemfibrozil significantly reduced serum TG levels (p , 0.005), yet HDL-C increased significantly only in HTG patients (p , 0.005). The changes in HDL-C levels were highly variable (-40 to 50%) and appeared to be dependent on the levels of serum TG achieved during treatment. Based on post-treatment serum TG, the HTG patients were divided into 2 groups. Group 1 with serum TG of ,100 mg/dL and Group 2 with serum TG levels ,100 mg/dl. Significant post treatment increases in HDL-C were seen only in Group 1 (p , ...
TY - JOUR. T1 - Genetic control of apolipoprotein A-I distribution among HDL subclasses. AU - Rainwater, David L.. AU - Blangero, John. AU - Moore, Perry H.. AU - Shelledy, Wendy R.. AU - Dyer, Thomas D.. PY - 1995. Y1 - 1995. N2 - We conducted genetic analyses to determine the components of variation for size distributions of apolipoprotein (apo) A-I among human plasma lipoproteins resolved on the basis of size. Analyses used data for 717 individuals in 26 pedigrees. Apo A-I distributions among lipoprotein size classes were measured by nondenaturing gradient gel electrophoresis (GGE) and immunoblotting procedures. Curves were fitted to apo A-I absorbance profiles to estimate fractional absorbance in each of five high-density lipoprotein (HDL) subclasses. Multivariate regression analyses revealed several covariates (sex, age, diabetes, and apo A-I concentrations) that were significantly associated with variation in one or more HDL subclasses. Female gender and elevated apo A-I concentrations ...
In human physiology, high-density lipoprotein (HDL) is a fraction of total serum cholesterol concerned with the retrieval and transport of lipid to the liver and other needy tissues. Since the conventional route of cholesterol distribution is from the liver to tissues, this process is often referred to as reverse cholesterol transport. HDL has gained a positive reputation and is referred to as "good cholesterol" since its presence is associated with a reduction in cardiovascular disease and mortality.. HDL particles are usually created by the liver and are small collections of several proteins, enzymes, and some phospholipid. Since cholesterol does not readily mix with the watery environment of blood, these components interact to create an efficient transport medium that will not stick to the sides of vessels. The majority of HDL particles are released into the blood as discs with minimal lipid content. As they fill up with excess cholesterol from cells like macrophages, they transform into ...
Phospholipid transfer protein (PLTP), which binds phospholipids and facilitates their transfer between lipoproteins in plasma, plays a key role in lipoprotein remodeling. PLTP levels increase during acute inflammation and increased PLTP activity is associated with the inflammatory markers C-reactive protein (CRP) and serum amyloid A (SAA) as seen in patients with type 2 diabetes and cardiovascular disease. SAA, an HDL apolipoprotein, is highly induced during inflammation. In this study we investigated whether HDL remodeling by PLTP is affected by SAA, and the significance of PLTP on HDL biogenesis. Over-expression of PLTP in mice using an adenoviral vector reduced HDL-C and phospholipid (PL) in a dose dependent manner with the liberation of lipid-poor apoA-I. Co-expression of PLTP and SAA produced a significantly greater reduction in HDL-C and PL than expression of either PLTP or SAA alone. Studies were carried out to examine the lipidation of apoA-I and formation of nascent HDL particles by ...
In this study, we show that CETP inhibition with anacetrapib affects HDL function, the HDL proteome, and metabolic outcomes differently depending on whether the mice were fed an obesogenic diet versus a chow diet. Anacetrapib treatment of CETP mice increased HDL cholesterol in chow- and HFD-fed mice. Increases in HDL cholesterol did not necessarily improve HDL function in chow-fed mice, which fits a growing consensus that HDL functionality is more predictive than HDL cholesterol levels with regard to protection from CVD. Proteomics technology has provided insight into the connections between HDL protein composition and functionality. We show a marked dietary influence on the modification of the HDL proteome by CETP inhibition with anacetrapib, which contributes to the functionality of HDL. Furthermore, inhibition of CETP in HFD-fed mice increased NEFA in the circulation and the liver and modified control of regulatory steps of TG metabolism in the liver, which was associated with increases in ...
Patti, Lidia, Swinburn, Boyd, Riccardi, Gabriele, Rivellese, Angela A. and Howard, Barbara V. 1991, Alterations in very low density lipoprotein subfractions in normotriglyceridemic non-insulin-dependent diabetics, Atherosclerosis, vol. 91, no. 1-2, pp. 15-23, doi: 10.1016/0021-9150(91)90183-4. ...
Apolipoprotein A-I (apo A-I) is the most abundant protein in high-density lipoprotein (HDL) particles, and it plays an important role in HDL metabolism. Both apo A-I and HDL cholesterol (HDL-C) levels are inversely associated with risk of cardiovascular disease. Segregation analyses suggest apo A-I levels are under the control of one or more major loci. Since HDL particles are heterogeneous in their composition and size, genetic influence on its subfractions (i.e., HDL2 and HDL3) could vary. A previous report showed evidence of a major locus controlling HDL3-C levels in a subset of the current study population. Because quantitative trait loci involved in complex diseases are likely to have pleiotropic effects on several related traits, it is possible to have a common major gene involved in regulating apo A-I and HDL3-C levels. We performed a bivariate segregation analysis of apo A-I and HDL3-C levels in 1,006 individuals from 137 families ascertained through probands undergoing elective, diagnostic
High - resolution two - dimension electrophoresis technique for protein with silver staining was used to characterise urinary high density lipoprotein (HDL) - apolipoproteins. Sequential ultracentrufugation method was used to isolate urinary lipoprotein particles of the same density as serum HDL. Immunostaining of electroblotted proteins further confirmed the presence of HDL-Apos in urine. HDL - Apolipoprotein A-I, A-II and C were identified in urine of normal subjects, diabetic patients and patients with biopsy proven glomerular proteinuria. An in-house ELISA method was used to quantify urinary HDL - Apo A - I. Selectivity indices were also determined.
BACKGROUND: New evidence shows that high density lipoproteins (HDL) have protective effects beyond their role in reverse cholesterol transport. Reconstituted HDL (rHDL) offer an attractive means of clinically exploiting these novel effects including cardioprotection against ischemia reperfusion injury (IRI). However, basic rHDL composition is limited to apolipoprotein AI (apoAI) and phospholipids; addition of bioactive compound may enhance its beneficial effects. OBJECTIVE: The aim of this study was to investigate the role of rHDL in post-ischemic model, and to analyze the potential impact of sphingosine-1-phosphate (S1P) in rHDL formulations. METHODS AND RESULTS: The impact of HDL on IRI was investigated using complementary in vivo, ex vivo and in vitro IRI models. Acute post-ischemic treatment with native HDL significantly reduced infarct size and cell death in the ex vivo, isolated heart (Langendorff) model and the in vivo model (-48%, p<0.01). Treatment with rHDL of basic formulation ...