The safety and immunogenicity of the vaccine with the antigen NY-ESO-1 in combination with the MPLA will be evaluated in a phase 1 clinical trial conducted in patients with malignancies that express the antigen (lung, ovarian and melanoma). This study will involve 15 patients, who will receive 250 mcg of NY-ESO-1 and 100 mcg of MPLA ...
Endotoxin-associated protein (EP) from Salmonella typhi activated murine resident peritoneal macrophages to produce prostaglandin E2 (PGE2). Cells from both endotoxin nonresponder (C3H/HeJ) and the endotoxin responder (C3H/OuJ) mouse strains were activated by EP. This EP-induced prostaglandin E2 production was blocked by the protein kinase C (PKC) inhibitor H-7 as well as the tyrosine kinase inhibitor genistein, suggesting the involvement of both serine and threonine phosphorylation and tyrosine phosphorylation pathways in the activation of resident peritoneal macrophages by EP. Immunoblot analysis using antiphosphoserine and antiphosphothreonine antibodies showed that EP induced the serine and threonine phosphorylation of a 14-kDa protein (p14). This phosphorylation was not induced by phorbol myristic acid or by lipopolysaccharide endotoxin. Inhibitors of PKC, PKA, and PKG did not block the phosphorylation of p14. However, the tyrosine kinase inhibitor piceatannol blocked p14 serine and ...
Adjuvant activity of monophosphoryl lipid A for nasal and oral immunization with soluble or liposome-associated antigen.: The effectiveness of monophosphoryl li
Background: Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. Objective: To compare effects of traditional cytokine cocktail (TNF-α + IL-1β) versus TLR4-agonist monophosphoryl lipid A on the viability, phenotype, cytokine profile and functionality of MDDC. Methods: The study included 32 individuals; twenty Acute Myeloid Leukaemia (AML) cases in complete remission and 12 healthy volunteers. They were divided into 3 groups: Group 1: control group: 12 subjects to measure the baseline levels of all markers in the monocytic preparation; Group 2: cytokine cocktail (TNF-α) group, which included 10 AML subjects; Group 3: MPLA group which included 10 AML subjects. Results: TNF-α group showed higher
One approach to improve the immunogenicity of an HIV-1 subunit protein vaccine is to combine the immunogen with an adjuvant. Adjuvants may augment vaccine immunogenicity by several mechanisms, and as a result induce a more favorable antibody response with high titers, which appear earlier in the course of immunization and persist over time.. Volunteers are randomized to receive 50 mcg rgp120/HIV-1SF2 in combination with one of seven different adjuvants: aluminum hydroxide (alum), monophosphoryl lipid A, liposome-encapsulated monophosphoryl lipid A, MF59, MTP-PE/MF59, Syntex adjuvant formulation (SAF/2), and SAF/2 plus threonyl muramyl dipeptide (threonyl MDP). An additional placebo control arm of volunteers receive alum only. Doses are administered at 0, 2, and 6 months. Volunteers are followed for 1 year after the last immunization. Per 8/5/94 amendment, eligible volunteers except those who received monophosphoryl lipid A for the first three immunizations may receive a fourth dose at month ...
For both E. coli and H. influenzae,lpxL mutants with predominantly tetra-acylated lipid A show temperature-sensitive growth; indeed, this phenotype was the basis for the identification of lpxL in the first place (11). We did not find this to be the case in N. meningitidis, as growth at 37 or 42°C was not different for the mutants compared to the wild type; instead, the lpxL2 mutant displayed a different conditional phenotype, i.e., the requirement for a truncated galactose-deficient oligosaccharide chain. Conceivably, a proper balance between the size of the hydrophobic and hydrophilic parts of LPS is required for maintenance of outer membrane stability. In both lpxL1 and lpxL2 mutants, the major OMPs PorA, PorB, and RmpM were observed in normal amounts, suggesting no major changes in outer membrane structure or composition (results not shown). The same lack of effect on expression of these OMPs was previously observed for a meningococcal lpxA mutant completely deficient in LPS due to a block ...
Catalyzes the N-acylation of UDP-3-O-(hydroxytetradecanoyl)glucosamine using 3-hydroxytetradecanoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Prefers (3R)-3-hydroxytetradecanoyl-ACP over (3R)-3-hydroxyhexadecanoyl-ACP as the acyl donor in vitro, which is consistent with the structure of E.coli lipid A that contains over 95% (R)-3-hydroxytetradecanoate at the 2 and 2 positions.
Vaccine adjuvants using heterogeneous monophosphoryl Lipid A (MPL) derived from Salmonella minnesota R595 have proven to be safe and effective at inducing Th-1 type immune responses to heterologous proteins in animal and human vaccines. Avanti revolutionized immunotherapy and vaccine development with the introduction of Synthetic MPL derivatives and adjuvant systems. Avanti now manufactures multiple synthetic analogs of MPL containing a single molecular species that are as effective and safe at inducing an immune response as their natural product predecessor. PHAD®, 3D-PHAD®, and 3D(6A)-PHAD® are manufactured according to cGMP guidelines and are available in bulk quantities for your clinical trials ...
The size distribution of adipocytes in a suspension, after collagenase digestion of adipose tissue, can be determined by computerized image analysis. Free lipid, forming droplets, in such suspensions implicates a bias since droplets present in the images may be identified as adipocytes. This problem is not always adjusted for and some reports state that distinguishing droplets and cells is a considerable problem. In addition, if the droplets originate mainly from rupture of large adipocytes, as often described, this will also bias size analysis. We here confirm that our ordinary manual means of distinguishing droplets and adipocytes in the images ensure correct and rapid identification before exclusion of the droplets. Further, in our suspensions, prepared with focus on gentle handling of tissue and cells, we find no association between the amount of free lipid and mean adipocyte size or proportion of large adipocytes. ...
With the development in extraction methods, more possibilities have been provided to exploration on applications of natural lipids. Natural lipids have potentials in food processing and health products manufacturing. However, the structural diversity is limited in natural lipids, as they are generated by organisms for certain biological functions. Therefore, natural lipids are often chemically modified to provide new properties and functions, thus known as modified lipids or semi-synthetic lipids. Modified lipids are useful tools in research, but limited in pharmaceutical and medical applications due to low thermostability and possible contamination from the animal or plant source. Synthetic lipids are chemically prepared from glycerol. The chemical route leads to even more diverse structures and high purity, which satisfy the special needs in the pharmaceutical industry.. ...
sample_1: DsbA, [U-100% 13C; U-100% 15N], 15 mg; H2O 90%; D2O 10%. sample_2: DsbA, [2-13C-glycerol; U-15N], 10 mg; H2O 90%; D2O 10%. sample_3: DsbA, [1,3-13C-glycerol; U-15N], 10 mg; H2O 90%; D2O 10%. sample_4: DsbB, [U-100% 13C; U-100% 15N], 7 mg; DDM 2 mg; E. coli lipids 7 mg; H2O 90%; D2O 10%. sample_5: DsbB, [2-13C-glycerol; U-15N], 5 mg; DDM 2 mg; E. coli lipids 7 mg; H2O 90%; D2O 10%. sample_6: DsbB, [1,3-13C-glycerol; U-15N], 4 mg; DDM 2 mg; E. coli lipids 7 mg; H2O 90%; D2O 10%. sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 270 K. sample_conditions_2: pH: 7.8; pressure: 1 atm; temperature: 261 K ...
LPS, கொழுப்பு-A (lipid-A) மற்றும் சர்க்கரை தொடரியைக் கொண்டது. கொழுப்பு-A பகுதியானது இம்மூலக்கூற்றின் நச்சு விளைவுகளுக்குக் காரணமாகும். சர்க்கரை தொடரியானது வெவ்வேறு பாக்டீரியாகளுக்கிடையில் அதிகமாக வேறுபட்டுக் காணப்படும். தோராயமாக, அகநச்சுகளின் அளவு 10 கிலோ டால்டன்கள் என்றாலும், அகநச்சுகள் 1000 கிலோ டால்டன்களுக்குச் சமமான திரட்டுகளை உருவாக்கும் தன்மை உள்ளதாகும். ...
The lipid A component of lipopolysaccharides from Fusobacterium nucleatum Fev 1 consists of beta-1,6-linked D-glucosamine disaccharides, which carry two phosphate groups: one in glycosidic and one in ester linkage. The amino groups of the glucosamine disaccharides are substituted by D-3-hydroxyhexadecanoic acid. The hydroxyl groups of the disaccharide backbone are acylated by tetradecanoic, hexadecanoic, and D-3-hydroxytetradecanoic acids. Part of the ester-bound D-3-hydroxytetradecanoic acid is 3-O-substituted by tetradecanoic acid. Whereas a similar pattern of fatty acids was detected in lipopolysaccharides from two other F. nucleatum strains, the amide-bound fatty acid in F. varium and F. mortiferum was D-3-hydroxytetradecanoic acid. The chemical relationships of lipid A from Fusobacteria and other gram-negative bacteria are discussed. ...
Although calreticulin (CRT) is a major Ca2+-binding luminal resident protein, it can also appear on the surface of various types of cells and it functions as an immunopotentiating molecule. However, molecular mechanisms underlying the potent immunobiological activity of cell surface CRT are still unclear. In the present study, a recombinant fragment (rCRT/39-272) covering the lectin-like N domain and partial P domain of murine CRT has been expressed in Escherichia coli. The affinity-purified rCRT/39-272 assembles into homodimers and oligomers in solution and exhibits high binding affinity to various glycans, including carrageenan, alginic acids, and hyaluronic acids. Functionally, rCRT/39-272 is capable of driving the activation and maturation of B cells and cytokine production by macrophages in a TLR-4-dependent manner in vitro. It specifically binds recombinant mouse CD14, but not BAFFR and CD40. It is also able to trigger Ig class switching by B cells in the absence of T cell help both in ...
Lipid A, the hydrophobic anchor of lipopolysaccharide (LPS), is an essential component in the outer membrane of Gram-negative bacteria. It can stimulate the innate immune system via Toll-like receptor 4/myeloid differentiation factor 2 (TLR4/MD2), leading to the release of inflammatory cytokines. In this study, six Escherichia coli strains which can produce lipid A with different acylation patterns were constructed; the influence of lipid A acylation pattern on the membrane permeability and innate immune stimulation has been systematically investigated. The lipid A species were isolated and identified by matrix assisted laser ionization desorption-time of flight/tandem mass spectrometry. N-Phenyl naphthylamine uptake assay and antibiotic susceptibility test showed that membrane permeability of these strains were different. The lower the number of acyl chains in lipid A, the stronger the membrane permeability. LPS purified from these strains were used to stimulate human or mouse macrophage cells, and
Chemical analyses and mass spectrometry were used to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas bestiarum strain K296, serotype O18. ESI-MS revealed that the most abundant A. bestiarum LPS glycoforms have a hexa-acylated or tetra-acylated lipid A with conserved architecture of the backbone, consisting of a 1,4′-bisphosphorylated β-(1→6)-linked d-GlcN disaccharide with an AraN residue as a non-stoichiometric substituent and a core oligosaccharide composed of Kdo1Hep6Hex1HexN1P1. 1D and 2D NMR spectroscopy revealed that the O-specific polysaccharide (OPS) of A. bestiarum K296 consists of a branched tetrasaccharide repeating unit containing two 6-deoxy-l-talose (6dTalp), one Manp and one GalpNAc residues; thus, it is similar to that of the OPS of A. hydrophila AH-3 (serotype O34) in both the sugar composition and the glycosylation pattern. Moreover, 3-substituted 6dTalp was 2-O-acetylated and additional O-acetyl groups were identified at O-2 and O-4 (or O-3) positions
The chemical structure of lipid A isolated from Porphyromonas gingivalis lipopolysaccharide was elucidated by compositional analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy. The hydrophilic backbone of free lipid A was found to consisted of beta(1,6)-linked D-glucosamine disaccharide 1-phosphate. (R)-3-Hydroxy-15-methylhexadecanoic acid and (R)-3-hydroxyhexadecanoic acid are attached at positions 2 and 3 of the reducing terminal residue, respectively, and positions 2 and 3 of the nonreducing terminal unit are acylated with (R)-3-O-(hexadecanoyl)-15-methylhexadecanoic acid and (R)-3-hydroxy-13-methyltetradecanoic acid, respectively. The hydroxyl group at position 4 is partially replaced by another phosphate group, and the hydroxyl groups at positions 4 and 6 are unsubstituted. Considerable heterogeneity in the fatty acid chain length and the degree of acylation and phosphorylation was detected by liquid secondary ion-mass spectrometry (LSI-MS). A significant ...
Despite considerable efforts toward vaccine development over decades, there is no available effective vaccine against Plasmodium vivax. Thrombospondin-related adhesive protein of P. vivax (PvTRAP) is essential for sporozoite motility and invasions into mosquitos salivary gland and vertebrates hepa …
Lipopolysaccharide (LPS)-binding protein (LBP) as an acute-phase protein plays a crucial role in innate host response to bacterial challenge. Our previous study shows that LBP expression in human gingiva is associated with periodontal status. Porphyromonas gingivalis is a keystone periodontopathogen, and its LPS with lipid A structural heterogeneity critically accounts for periodontal pathogenesis. This study investigated the effects of LBP and its interactions with two featured isoforms of P. gingivalis LPS (tetra-acylated LPS1435/1449 and penta-acylated LPS1690) on the expression of pro-inflammatory cytokines in human oral keratinocytes (HOKs), and the involvement of Toll-like receptor (TLR) signaling. HOKs were pre-incubated with recombinant human LBP (rhLBP) at 10ng/ml, 100ng/ml and 1μg/ml for 1 h, followed by the treatment of P. gingivalis LPS1690 or LPS1435/1449 for 3h or 24h respectively. The expression of IL-6 and IL-8, and involvements of TLR2 and TLR4 were analyzed. The genes associated with
Monophosphoryl Lipid A (MPLA), a derivative of LPS endotoxin, is a TLR4 agonist that displays as little as 0.1-1% as much toxicity as its parent molecule while retaining immunostimulatory properties. We discovered that MPLA activates a TRIF-biased pattern of TLR4 signaling, resulting in reduced production of MyD88-dependent pro-inflammatory factors, and credited TRIF-bias for MPLAs reduced toxicity. A contemporary study showed that MPLA fails to promote maturation of the potent inflammatory cytokine IL-1ß. This dissertation seeks to reconcile MPLAs TRIF-biased signaling with IL-1 ß loss, and to determine the ultimate cause of MPLAs reduced toxicity compared to LPS. We find that TRIF-biased TLR4 activation results in weak MyD88-dependent induction of NLRP3, a critical inflammasome component required for IL-1 ß production. MPLAs loss of IL-1 ß results in decreased potentiation of MyD88-dependent inflammatory factors in vivo and reduced IL-1 RI-dependent hepatotoxicity. Ultimately, TRIF-biased TLR4
LPS has been associated with obesity and type 2 diabetes in mice and humans9 ,12 and subcutaneous administration of LPS promotes adiposity and a slight impairment of glucose metabolism.11 However, little is known about how LPS from the gut affects metabolism. We therefore investigated the importance of gut-derived LPS in mediating glucose and insulin tolerance and macrophage accumulation and inflammation by colonising GF mice with either E.coli W3110 or the isogenic mutant MLK1067, a strain with reduced immunogenicity owing to its penta-acylated lipid A component,15 for 4 weeks. We first confirmed that the mutant exhibited reduced capacity to induce Tnfα expression and tumour necrosis factor α secretion in murine RAW 264.7 macrophages (online supplementary figure S2A,B), and showed that W3110 and MLK1067 colonised the mouse gut approximately to the same bacterial density (1.1×1010 and 1.5×1010 CFU/ml for W3110 and MLK1067, respectively).. As expected, colonisation with E.coli W3110 but not ...
Polysaccharides comprise a distinct class of biopolymers produced ubiquitously among living organisms. They play significant roles in modern science and technology and bestow several advantages to mankind ranging from achieving desirable food texture to serving as tissue scaffolds. Their recognition by Toll-like receptors that are key regulators of innate immunity toward responding to invading microorganisms as well as observation of immunopharmacological activity dependence on the polysaccharide conformation accentuates the essential need of structural information for deciphering the biological behavior. Their unique properties such as renewability and biodegradability further spawn the design and development of new biomaterial products namely hydrogels and implants that have the ability to respond to specific stimuli with desired pharmacokinetics. These functional behaviors rely greatly on polysaccharides molecular organizations as well as their time dependent structural transformations. ...
Another one in the series of endotoxin antagonism featuring so far vitamins D and B2. Vitamin B2 (riboflavin) Is Endotoxin Antagonist Vitamin B2...
Поиск диссертаций, авторефератов, научных статей и публикаций в русскоязычных источниках и периодике. LpxM(msbB) mutant
High Quality D-Glucosamine Hydrochloride Supplier, FOODCHEM is Top D-Glucosamine Hydrochloride Supplier and Manufacturer in China for more than 10 years, Buy D-Glucosamine Hydrochloride Food Grade and Feed Grade at Foodchem
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 14999-44-1(D-Glucosamine Hydroiodide),please inquire us for 14999-44-1(D-Glucosamine Hydroiodide).
Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Dufrisne MBelcher, Kloss B, et al. Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation. Science. 2016 ;351(6273):608-12. ...
Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Dufrisne MBelcher, Kloss B, et al. Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation. Science. 2016 ;351(6273):608-12. ...
柔潤劑、乳化劑,飽和性脂肪酸,棕櫚油中之主要成份,與強鹼類皂化後即形成肥皂。 柔潤劑, 乳化劑 ...
Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disorder characterized by broad clinical manifestations including cardiovascular and renal complications with periodic disease flares and significant morbidity and mortality. One of the main contributing factors to the pathology of SLE is the accumulation and impaired clearance of immune complexes of which the principle components are host auto-antigens and antibodies. The contribution of host lipids to the formation of these autoimmune complexes remains poorly defined. The aim of the present study was to identify and analyze candidate lipid autoantigens and their corresponding anti-lipid antibody responses in a well-defined SLE patient cohort using a combination of immunological and biophysical techniques. Disease monitoring in the SLE cohort was undertaken with serial British Isles Lupus Assessment Group (BILAG) scoring. Correlations between specific lipid/anti-lipid responses were investigated as disease activity developed from active flares
Catalyzes the ATP-dependent phosphorylation of the 3-deoxy-D-manno-octulosonic acid (Kdo) residue in Kdo-lipid IV(A) at the 4-OH position.
Gram negative and positive bacteria have evolved toxins to aid in their ability to colonize host organisms. Some gram-positive bacteria produce exotoxins called superantigens that hyper-stimulate the immune system by crosslinking the variable region of the beta chain (Vβ) of T cell receptors with the antigen presenting major histocompatibility complex II molecule on the surface of antigen presenting cells. This hyper-stimulation leads to overproduction of cytokines, which can result in toxic shock. In addition, the action of the superantigens has been implicated in many diseases including necrotizing pneumonia and endocarditis. Gram-negative bacteria produce lipopolysaccharide (LPS), also called endotoxin, as a major constituent in their outer cell walls. LPS binds to the host protein called MD-2 and the LPS:MD-2 complex associates with cell surface homodimeric Toll-like receptor 4 (TLR4). This tri-molecular interaction can lead to massive stimulation of cytokines from TLR4+ antigen presenting ...
Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and...
We,China D-Glucosamine Hydrochloride 66-84-2 Suppliers and China D-Glucosamine Hydrochloride 66-84-2 Manufacturers, provide D-Glucosamine Hydrochloride 66-84-2 product and the products related with China D-Glucosamine Hydrochloride 66-84-2 - zjzhsw
China D-Glucosamine Sulphate 2nacl, Find details about China D-Glucosamine Sulphate 2nacl, Glucosamine from D-Glucosamine Sulphate 2nacl - Yangzhou Rixing Bio-Tech Co., Ltd.
A - Tilt: 3° - Segments: 1( 28- 47), 2( 105- 126), 3( 135- 153), 4( 158- 175), 5( 186- 203), 6( 205- 221), 7( 233- 250), 8( 282- 304), 9( 321- 337), 10( 343- 358), 11( 369- 389), 12( 406- 427), 13( 434- 453 ...
China D-Glucosamine HCl, Find details about China Glucosamine, D Glucosamine from D-Glucosamine HCl - Qingdao Header International Trading Co., Ltd.
The bacterial lipopolysaccharide also known as endotoxin is exhaustively covered in the present work. Central emphasis is placed upon the fine chemical structure of the lipopolysaccharide and its sign
USP,PVP K-30,D-Glucosamine HCl(DC Grade),US $ 1 - 100 / Kilogram, Shaanxi, China (Mainland), Antioxidants, GKSW.Source from Baoji Guokang Bio-Technology Co., Ltd. on Alibaba.com.
Lipid Soluble, Wholesale Various High Quality Lipid Soluble Products from Global Lipid Soluble Suppliers and Lipid Soluble Factory,Importer,Exporter at Alibaba.com.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
To battle against premature aging, three essential lines of protection and correction exist that are crucial for the maintenance of the skins natural beauty, including: shielding against UV radiation; protecting the skins natural lipid structure; and counteracting internal damage.
Deoarece si noi, in interior, de abia ne miscam. Aspectele aferente terapiei CranioSacrale sunt extrem de subtile. Necesita exercitiu, dar, o data însusite, ramân pe viata.. De ce se simt unii oameni mai rau, în urma unui tratament?. Foarte rar se întâlnesc cazuri în care clientul se poate simti mai rau dupa terapie si asta se datoreaza faptului ca poate, în timpul terapiei, odata cu relaxarea tesutului conjunctiv, clientul retraieste din punct de vedere emotional momentul în care trauma s-a produs, sau corpul lor re-experimenteaza pe masura eliberarii acesteia din tesuturi, o trauma anterioara sau o ranire si asta dureaza câteva zile pâna se disipeaza complet, sau poate corpul s-a obisnuit cu "raul" si-i ia timp sa se obisnuiasca cu "binele" . Se mai numeste si "criza vindecarii".. Un alt motiv poate fi acela conform caruia zona de "amorteala" a revenit la "viata" si a devenit mai sensibila. De asemeni, se întâmpla adeseori ca un corp sa se adapteze unui defect. Atunci când ...
... Aging skin is increasingly susceptible to lipid depletion; the loss of natural compounds in skin s surface,
Bridging the gap between the lab and the clinic, Lipids in Health and Diseases publishes high-quality, high-volume research on all aspects of lipids. We ...
We explain Introduction to Lipids with video tutorials and quizzes, using our Many Ways(TM) approach from multiple teachers.|p| This lesson will introduce the basic structure and function of a lipid.|/p|
The NLA was created out of the success established by the Southeast Lipid Association (SELA), which was formed in 1997 by a group of pioneering lipid ...
Akhilesh Bajpai and Sravanthi Davuluri (Correspondence: Acharya KK, [email protected]), A list of resources for lipids; In: Startbioinfo; 09 Sep 2012, http://www.shodhaka.com/cgi-bin/startbioinfo/prelimresources.pl?tn=Lipids" ...
Editor Nick Wakeman shares six questions he plans to ask during a roundtable discussion on lowest price, technically acceptable contracting, the dreaded LPTA. Is he taking the conversation in the right direction?
0 yrs old Male asked about 350 Lipid profile, 4 doctors answered this and 76 people found it useful. Get your query answered 24*7 only on | Practo Consult