TY - JOUR. T1 - Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism. AU - Shirakawa, Takashi. AU - Nakajima, Katsuyuki. AU - Shimomura, Younosuke. AU - Kobayashi, Junji. AU - Stanhope, Kimber. AU - Havel, Peter J. AU - Machida, Tetsuo. AU - Sumino, Hiroyuki. AU - Murakami, Masami. PY - 2015/2/2. Y1 - 2015/2/2. N2 - Background: A comparison of post-heparin and pre-heparin plasma lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) on the metabolism of remnant lipoproteins (RLPs) has not been reported yet. Methods: Healthy volunteers were injected with heparin for LPL and HTGL determination in the fasting (8:00) and postprandial (20:00) plasma on the same day. Plasma total cholesterol (TC), triglycerides (TG), LDL-C, HDL-C, small dense LDL (sdLDL)-C, remnant lipoprotein (RLP)-C, RLP-TG, the RLP-TG/RLP-C ratio, adiponectin and apoCIII were measured. Results: LPL activity and concentration in the ...
In a lipase investigation, Candida antarctica lipase B (CALB) are explored for enzyme catalytic promiscuity. Enzyme catalytic promiscuity is shown by enzymes catalyzing alternative catalytic transformations proceeding via different transition state structures than normal. CALB normally performs hydrolysis reactions by activating and coordinating carboxylic acid/ester substrates in an oxyanion hole prior to nucleophilic attack from an active-site serine resulting in acyl enzyme formation. The idea of utilizing the carbonyl activation oxyanion hole in the active-site of CALB to catalyze promiscuous reactions arose by combining catalytic and structural knowledge about the enzyme with chemical imagination. We choose to explore conjugate addition and direct epoxidation activities in CALB by combining molecular modeling and kinetic experiments. By quantum-chemical calculations, the investigated promiscuous reactions were shown to proceed via ordered reaction mechanisms that differ from the native ping ...
The synthetic gene of CalBsyn was previously constructed to encode Candida antarctica lipase B (CALB). Lipase of CalBsyn gene is slightly different from...
Reaction rates and selectivities were measured for transacylation of fatty acid esters in solvents catalysed by Candida antarctica lipase B and by cutinase from Humicola insolens. With these enzymes classical water-based enzymology can be expanded to many different solvents allowing large variations in interaction energies between the enzymes, the substrates and the surrounding. Further ,hydrolysis reactions catalysed by Bacillus subtilis esterase 2 were investigated.. Thermodynamics analyses revealed that the enzyme contribution to reaction rate acceleration compared to acid catalysis was purely entropic. On the other hand, studies of differences in activation entropy and enthalpy between enantiomers and between homologous esters showed that high substrate specificity was favoured by enthalpic stabilisation.. Solvent was found to have a profound effect on enzyme catalysis, affecting both reaction rate and selectivity. Differences in substrate solubility will impact enzyme specificity since ...
The structure and flexibility of Candida antarctica lipase B in water and five different organic solvent models was investigated using multiple molecular dynamics simulations to describe the effect of solvents on structure and dynamics. Interactions of the solvents with the protein and the distribution of water molecules at the protein surface were examined. The simulated structure was independent of the solvent, and had a low deviation from the crystal structure. However, the hydrophilic surface of CALB in non-polar solvents decreased by 10% in comparison to water, while the hydrophobic surface is slightly increased by 1%. There is a large influence on the flexibility depending on the dielectric constant of the solvent, with a high flexibility in water and a low flexibility in organic solvents. With decreasing dielectric constant, the number of surface bound water molecules significantly increased and a spanning water network with an increasing size was formed. The reduced flexibility of Candida
Hepatic lipase, also called hepatic triglyceride lipase (HTGL) or LIPC (for "lipase, hepatic"), is a form of lipase, catalyzing the hydrolysis of fats. It is expressed in the liver and adrenal glands. One of the principal functions of hepatic lipase is to convert intermediate-density lipoprotein (IDL) to low-density lipoprotein (LDL). LIPC encodes hepatic triglyceride lipase (HTGL), which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Hepatic lipase deficiency is a rare, autosomal recessive disorder that results in elevated high density lipoprotein (HDL) cholesterol due to a mutation in the hepatic lipase gene. Clinical features are not well understood and there are no characteristic xanthomas. There is an association with a delay in atherosclerosis in an animal model. GRCh38: Ensembl release 89: ENSG00000166035 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000032207 - Ensembl, May 2017 ...
TY - JOUR. T1 - Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. AU - See Meng, Lim. AU - Goh, Yong Meng. AU - Kuan, Wen Bin. AU - Loh, Su Peng. PY - 2014/11/3. Y1 - 2014/11/3. N2 - Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes. Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement. Results: GBR extract using hexane showed the highest ...
The gene encoding lipase B from Candida antarctica (CalB) was expressed in Pichia pastoris after it was synthesized by the recursive PCR and cloned into the Pichia expression plasmid, pPICZαA. The CalB was successfully secreted in the recombinant P. pastoris strain X-33 with an apparent molecular weight of 34 kDa. For 140 h flask culture, the dry cell weight and the extracellular lipase activity reached at 5.4 g/l and 57.9 U/l toward p-nitrophenyl palmitate, respectively. When we performed the fed-batch fermentation using a methanol feeding strategy for 110 h, the dry cell weight and the extracellular lipase activity were increased to 135.7 g/l and 11,900 U/l; the CalB protein concentration was 1.18 g/l of culture supernatant. The characteristics of CalB recovered from the P. pastoris culture were compared with the commercial form of CalB produced in Aspergillus oryzae. The kinetic constants and specific activity, the effects of activity and stability on temperature and pH, the glycosylation extent,
The triglyceride lipase gene subfamily plays a central role in lipid and lipoprotein metabolism. There are three members of this subfamily: lipoprotein lipase, hepatic lipase, and endothelial lipase. Although these lipases are implicated in the pathophysiology of hyperlipidemia and atherosclerosis, their structures have not been fully solved. In the current study, we established homology models of these three lipases, and carried out analysis of their activity sites. In addition, we investigated the kinetic characteristics for the catalytic residues using a molecular dynamics simulation strategy. To elucidate the molecular interactions and determine potential key residues involved in the binding to lipase inhibitors, we analyzed the binding pockets and binding poses of known inhibitors of the three lipases. We identified the spatial consensus catalytic triad "Ser-Asp-His", a characteristic motif in all three lipases. Furthermore, we found that the spatial characteristics of the binding pockets ...
DISCUSSION The essential role of oxygen in lipid metabolism and cell growth is well known. Moreover, lipase production by several microorganisms depends on the availability of oxygen. Although in most cases oxygen seems to favor lipase production, low levels of aeration have also been reported to increase production of the enzyme (Corzo and Revah, 1999). It is often difficult to distinguish the effects of changes in stirring speed from changes in air flow rate. Both DO concentration and OTR have been used to correlate rates of aerobic fermentation. The OTR, however, is not constant during fermentation, varying with time due to the change in DO in the fermentation medium. Thus, kLa values can be used to establish the relationship between OTR and lipase production (Elibol and Ozer, 2000). It was clearly observed that an increase in stirring speed, rather than air flow rate, resulted in the most pronounced increase in kLa, as expected for a mechanically stirred vessel. Maximum extracellular lipase ...
Lipase inhibitors are substances used to reduce the activity of lipases found in the intestine. Lipases are secreted by the pancreas when fat is present. The primary role of lipase inhibitors is to decrease the gastrointestinal absorption of fats. Fats then tend to be excreted in feces rather than being absorbed to be used as a source of caloric energy, and this can result in weight loss in individuals. These inhibitors could be used for the treatment of obesity, which can subsequently lead to Type II diabetes and cardiovascular diseases if not managed. An example of a lipase inhibitor is orlistat. Lipase inhibitors may affect the amount of fat absorbed, yet they do not block the absorption of a particular type of fat. Likewise, lipase inhibitors are not absorbed into the bloodstream. Lipase inhibitors bind to lipase enzymes in the intestine, thus preventing the hydrolysis of dietary triglycerides into monoglycerides and fatty acids. This then reduces the absorption of dietary fat. Lipase ...
The bacterial thermoalkalophilic lipases optimally hydrolyze saturated fatty acids at elevated temperatures. They also have significant sequence homology with staphylococcal lipases, and both the thermoalkalophilic and staphylococcal lipases are grouped as the lipase family 1.5. We report here the first crystal structure of the lipase family 1.5, the structure of a thermoalkalophilic lipase from Bacillus stearothermophilus L1 (L1 lipase) determined at 2.0-? resolution. The structure is in a closed conformation, and the active site is buried under a long lid helix. Unexpectedly, the structure exhibits a zinc-binding site in an extra domain that accounts for the larger molecular size of the family 1.5 enzymes in comparison to other microbial lipases. The zinc-coordinated extra domain makes tight interactions with the loop extended from the C terminus of the lid helix, suggesting that the activation of the family 1.5 lipases may be regulated by the strength of the interactions. The unusually long ...
Enzymes For Leather Alkaline Lipase JZ, Parameters: Appearance :White Powder, Components : Alkaline Lipase and special additives,&#...
A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 °C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca2+ and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 °C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa. ...
The influence of water on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is percolated by gaseous nitrogen, simultaneously carrying gaseous substrates to the enzyme while removing reaction products. In this system, interactions between the enzyme and non-reacting molecules are avoided, since no solvent is present, and it is thus more easy to assess the role of water. To this end, alcohol inhibition constant, substrates dissociation constants as well as acylation rate constant and ratio of acylation to deacylation rate constants have been determined as a function of water activity (aW). Data obtained highlight that n-propanol inhibition constant and dissociation constant of methyl propionate are a lot affected by aW variations whereas water has no significant effect on the catalytic acylation step nor on
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TY - JOUR. T1 - A case of non-alcoholic steatohepatitis complicated with severe acute pancreatitis induced by decreased lipoprotein lipase and hepatic triglyceride lipase activity levels in a young Japanese woman. AU - Minohara, Sawa. AU - Bae, Sung Kwan. AU - Sugiyama, Saori. AU - Shibata, Noriko. AU - Gushima, Toshifumi. AU - Motoshita, Junichi. AU - Shimoda, Shinji. AU - Takagi, Atsuko. AU - Ikeda, Yasuyuki. AU - Takahashi, Kazuhiro. PY - 2018/9. Y1 - 2018/9. N2 - We report a case of non-alcoholic steatohepatitis complicated with acute pancreatitis induced by hypertriglyceridemia in a young Japanese woman. A precise examination of the lipid profile showed decreased lipoprotein lipase (LPL) and hepatic triglyceride lipase activity levels, while the LPL mass was at the minimum level of the normal range.. AB - We report a case of non-alcoholic steatohepatitis complicated with acute pancreatitis induced by hypertriglyceridemia in a young Japanese woman. A precise examination of the lipid profile ...
TY - JOUR. T1 - The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. AU - Kim, Kyeong Kyu. AU - Song, Hyun Kyu. AU - Shin, Dong Hae. AU - Hwang, Kwang Yeon. AU - Sun, Se Won. PY - 1997/2/15. Y1 - 1997/2/15. N2 - Background: Lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. True lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. Lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. Their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of enantiomerically pure pharmaceuticals. Recent structural studies on several lipases have provided some clues towards understanding the mechanisms of hydrolytic activity, interfacial activation, and ...
The production of commercial enzymes, including lipase from bacteria has always been the industrial choice due to its economical and commercial feasibility. Lipases are produced by microorganisms such as bacteria and fungi. However, we have focused on bacterial microbial lipases were economically importance of several properties. The present studies of the goals of this paper were isolation and identification of lipase producing bacteria from Oil contaminated soil. Screening and isolation of lipase producing strains of bacteria was carried out from different soil samples collected from various area in Surat, Gujarat, India. Tributyrin agar, selective media for isolation of lipase producing bacteria was used. Out of 41 isolates,20 were showing lipolytic activity. Agar well diffusion method used for further screening of best isolate for lipase production. The isolate AD1 with maximum zone of 27mm on Phenol red agar plate produce the lipase with the activity of 4.27U/ml obtained after 96hrs of incubation
Fat tissue is the most important energy depot in vertebrates. The release of free fatty acids (FFAs) from stored fat requires the enzymatic activity of lipases. We showed that genetic inactivation of adipose triglyceride lipase (ATGL) in mice increases adipose mass and leads to triacylglycerol deposition in multiple tissues. ATGL-deficient mice accumulated large amounts of lipid in the heart, causing cardiac dysfunction and premature death. Defective cold adaptation indicated that the enzyme provides FFAs to fuel thermogenesis. The reduced availability of ATGL-derived FFAs leads to increased glucose use, increased glucose tolerance, and increased insulin sensitivity. These results indicate that ATGL is rate limiting in the catabolism of cellular fat depots and plays an important role in energy homeostasis.. ...
The structural gene (lipA) coding for the extracellular lipase of Pseudomonas aeruginosa PAO1 has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. lipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was predicted to have a M r of 30134, an isoelectric point of 5.6, and a consensus sequence (IGHSHGG) typical of lipases. Furthermore it is highly homologous (>60%) to other lipases from various pseudomonads. The lipA gene failed to hybridize detectably with genomic DNA from other Pseudomonas species except P. alcaligenes, even under relaxed stringency. Located 220 bp downstream of the lipA gene, is an open reading frame (ORF2, lipH) which encodes a hydrophilic protein (283 amino acids; M r 33587) that shows some homology to the limA gene product of P. cepacia. In complementation tests of lipase-defective mutants, lipH was shown to be necessary for expression of active extracellular lipase in P. aeruginosa
A novel lipase gene lip5 from the yeast Candida albicans was cloned and sequenced. Alignment of amino acid sequences revealed that 86-34% identity exists with lipases from other Candida species. The lipase and its mutants were expressed in the yeast Pichia pastoris, where alternative codon usage caused the mistranslation of 154-Ser and 293-Ser as leucine. 154-Ser to leucine resulted in loss of expression of Lip5, and 293-Ser to leucine caused a marked reduction in the lipase activity. Lip5-DM, which has double mutations that revert 154 and 293 to serine residues, showed good lipase activity, and was overexpressed and purified by (NH4)2SO4 precipitation and ion-exchange chromatography. The pure Lip5-DM was stable at low temperatures ranging from 15-35 °C and pH 5-9, with the optimal conditions being 15-25 °C and pH 5-6. The activation energy of recombinant lipase was 8.5 Kcal/mol between 5 and 25 °C, suggesting that Lip5-DM was a cold-active lipase. Its activity was found to increase in the presence
The principal objectives of this study were to evaluate the kinetics of lipase production by Staphylococcus warneri EX17 under different oxygen volumetric mass…
extracellular region, extracellular space, lipase activity, triglyceride lipase activity, lipid digestion, lipid metabolic process, positive regulation of triglyceride lipase activity, retinoid metabolic process
Endothelial lipase is a form of lipase secreted by vascular endothelial cells in tissues with high metabolic rates and vascularization, such as the liver, lung, kidney, and thyroid gland.[1] Unlike the lipases that hydrolyze triglycerides, endothelial lipase primarily hydrolyzes phospholipids.[1] It was first characterized in 1999.[2] ...
The Lipase Engineering Database is online ! The Lipase Engineering Database (LED) is a WWW-accessible resource on sequence-structure-function relationships of microbial lipases. Sequences of 92 microbial lipases and homologous serine hydrolases were assigned to 32 homologous families and 15 superfamilies. Multisequence alignments of all homologous families and superfamilies are provided. Functionally relevant amino acids are annotated. The complete catalytic machinery (catalytic triad and two oxyanion hole residues) could be annotated in 91 % of LED sequence entries. Published mutants and their properties are provided. The X-ray structures of 22 lipases were superposed and annotated. Consistently annotated aligned sequences and superimposed structures of microbial lipases help to understand the functional role of individual amino acids and thus the LED is a useful tool for protein engineering. We have also set up a mailing list as a forum for discussions and announcements on topics related to ...
Introduction: Myocardial metabolism undergoes change in response to pathological cardiac hypertrophy (PH), characterized by increased reliance on glucose oxidation, decreased free fatty acid (FFA) oxidation and a loss of metabolic flexibility. Cardiac metabolism is influenced by other organs such as adipose tissue. Hence, we aimed to investigate the effect of Adipose Triglyceride Lipase (ATGL) in adipose tissue on the development of PH and heart failure (HF) in a pressure overload-induced cardiac hypertrophy model in mice.. Methods: Male adipose tissue specific ATGL-knock out (atATGL-KO) and wild type mice (WT) underwent sham surgery (sham) or transverse aortic constriction (TAC). After 11 weeks, mice were sacrificed and organs were harvested.. We performed echocardiography one week before and 11 weeks after surgery. Left ventricular mass (LVM), left ventricular mass/tibia length (LVM/TL) and ejection fraction (EF) were calculated. Beta-myosin heavy chain (β-MyHC) was measured in RNA of hearts. ...
Background. Serum lipase and amylase are biochemical analyses used to establish the diagnosis of acute pancreatitis (AP). Despite lipase having been shown internationally to be a more sensitive and specific test, amylase remains a popular first-line test. Objective. To provide a local basis for the recommendation of the best first-line laboratory test, an assessment of their performance in our local setting was undertaken. Methods. From a prospective dataset on patients with acute abdominal pain and raised serum lipase and/or amylase values, the sensitivity and specificity of serum lipase, amylase and the two in combination was calculated for the diagnosis of AP, as defined by the Atlanta criteria. Results. During the study period, 476 patients presented with acute upper or generalised abdominal pain and raised serum amylase and/or lipase values. The median age of the patients was 43 years (range 14 - 85), and 58% were men and 42% women. Of the patients, 322 (68%) presented with abdominal conditions
Creative Enzymes (US). The report begins with the overview of the Bacteria Sourced Microbial Lipase Market and offers throughout development. It presents a comprehensive analysis of all the regional and major player segments that gives closer insights upon present market conditions and future market opportunities along with drivers, trending segments, consumer behaviour, pricing factors and market performance and estimation throughout the forecast period.. The report also covers geographical markets and key players that have adopted significant strategies for business developments. The data within the report is displayed in a statistical format to offer a better understanding upon the dynamics. The report compiles exhaustive information acquired through proven research methodologies and from dedicated sources across several industries.. To Buy Full Version Of This Report, Visit https://dataintelo.com/checkout/?reportId=127302. The report segments the Global Bacteria Sourced Microbial Lipase ...
Phosphorylation of adipose triglyceride lipase Ser(404) is not related to 5-AMPK activation during moderate-intensity exercise in humans
Enzyme selectivity means that the enzyme´s preferences towards competing substrates will be different. In this thesis, the enzyme selectivity has been studied for utilization in synthesis of functionalized macromonomers. The aim was to study how the inherent -or introduced - selectivity of lipases can be used to introduce thiol‐ or enefunctionalities into short polyesters. Thiol‐ and ene‐functionalized renewable organic precursor molecules in combination with thiol‐ene click chemistry opens up for a sustainable material production. Lipases do not normally affect ene‐moieties and the preference towards thiols is low, enabling introduction of these functional groups for further modifications. In addition, lipases have been shown to be good catalysts in the formation of polyesters, both via ring‐opening and polycondensation polymerization.. In paper I Candida antarctica lipase B was used to end‐functionalize poly(ε‐caprolactone) with free thiols in a one‐pot reaction. The ...
In nature, lipases (EC 3.1.1.3) catalyze the hydrolysis of triglycerides to form glycerol and fatty acids. Under the appropriate conditions, the reaction is reversible, and so biotechnological applications commonly make use of their capacity for esterification as well as for hydrolysis of a wide variety of compounds. In the present paper, we report the X-ray structure of lipase A from Candida antarctica, solved by single isomorphous replacement with anomalous scattering, and refined to 2.2-Å resolution. The structure is the first from a novel family of lipases. Contrary to previous predictions, the fold includes a well-defined lid as well as a classic α/β hydrolase domain. The catalytic triad is identified as Ser184, Asp334 and His366, which follow the sequential order considered to be characteristic of lipases; the serine lies within a typical nucleophilic elbow. Computer docking studies, as well as comparisons to related structures, place the carboxylate group of a fatty acid product near ...
The present work describes a simple and sensitive spectrophotometric method based on the release of p-nitrophenyl butyrate (p-NPB) for the estimation of lipase activity. The method was optimized and validated in biphasic complex media containing methyl ricinoleate (MR) or olive mill wastewater (OMW), although it may be used for other biphasic media. Reaction mixtures containing substrate (2.63 mM p-NPB in sodium acetate buffer, 0.05 M and pH 5.6, with 4% (v/v) Triton X-100) and lipase are incubated at 37 ºC during 15 minutes. After this time, 2 mL of acetone are added to stop the reaction and the corresponding absorbances are measured at 405 nm in a microplate reader. A linear response was obtained for Candida rugosa lipase concentrations in the range of 0.0054 to 0.1 g L-1 and 0.093 to 0.5 g L-1 for MR and OMW media, respectively. The method revealed to be more sensitive for MR medium. This conclusion was corroborated by the detection and quantification limits, which were smaller for MR than ...
TY - JOUR. T1 - Effect of gastric lipase on turbidimetric and dry-film methods for measuring pancreatic lipase [1]. AU - Kazmierczak, S. C.. AU - Van Lente, F.. AU - Tetrault, G. A.. PY - 1992/12/1. Y1 - 1992/12/1. UR - http://www.scopus.com/inward/record.url?scp=0027098002&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027098002&partnerID=8YFLogxK. M3 - Letter. C2 - 1458599. AN - SCOPUS:0027098002. VL - 38. SP - 2555. EP - 2556. JO - Clinical Chemistry. JF - Clinical Chemistry. SN - 0009-9147. IS - 12. ER - ...
REFERENCES. 1 Maruyama T, Nakajima M, Ichikawa S, Nabetani H, Furusaki S, Seki M. Oil-water interfacial activation of lipase for interesterification of triglyceride and fatty acid. J Am Oil Chem Soc. 2000;77:1121-1127. [ Links ] 2 Verma ML, Azmi W, Kanwar SS. Microbial lipases: at the interface of aqueous and non-aqueous media. A review. Acta Microbiol Immunol Hung. 2008;55:265-294. [ Links ] 3 Belfrage P, Fredrikson G, Stralfors P, Tornquist H. In: Borgstrom B, Brockman HL, eds. Lipases. Amsterdam: Elsevier Science; 1984:365-416. [ Links ] 4 Akoh CC, Sellappan S, Fomuso LB, Yankah G V.V. Enzymatic synthesis of structured lipids. In: Kuo TM, Gardner HW, eds. Lipid Biotechnology. New York, USA: Marcel Dekker;2002:433-460.5. [ Links ] 5 Saxena RK, Sheoran A, Giri B, Davidson SW. Purification strategies for microbial lipases. J Microbiol Methods. 2003;52:1-18. [ Links ] 6 Koops BC, Verheij HM, Slotboom AJ, Egmond MR. Effect of chemical modification on the activity of lipases in organic solvents. ...
Utilization of coconut oil mill waste as a substrate for optimized lipase production, oil biodegradation and enzyme purification studies in Staphylococcus pasteuri
GOMBERT, Andreas Karoly; PINTO, A L; CASTILHO, L R; FREIRE, D M G. Lipase production by Penicillium Restrictum in solid-state fermentation using babassu oil cake as substrate. Process Biochemistry, London, v. 35, n. 1/2, p. 85-90, 1999. DOI: 10.1016/s0032-9592(99)00036-9 ...
Burkholderia cepacia lipase (BCL) has been proved to be capable of resolution reactions. However, its free form usually exhibits low stability, bad resistance and no reusability, which restrict its further industrial applications. Therefore, it is of great importance to improve the catalytic performance of free lipase in non-aqueous medium. In this work, macroporous resin NKA (MPR-NKA) was utilized as support for lipase immobilization. Racemic transesterification of 1-phenylethanol with vinyl acetate was chosen as model reaction. Compared with its free form, the enzyme activity and enantioselectivity (ees) of the immobilized lipase have been significantly enhanced. The immobilized BCL exhibited a satisfactory thermostability over a wide range of temperature (from 10 to 65°C) and an excellent catalytic efficiency. After being used for more than 30 successive batches, the immobilized lipase still kept most of its activity. In comparison with other immobilized lipases, the immobilized BCL also exhibits
Human gastric lipase (hGL) is a member of the family of preduodenal lipases, primarily responsible for initiation of lipolysis of dietary fats, such as triglycerides in the gastrointestinal tract. It is a candidate for treatment of pancreatic enzyme insufficiency, as a superior substitute for the current treatment that uses enzyme obtained from porcine pancreatic extract. Recently, it was demonstrated that active recombinant hGL can be produced in N. benthamiana by transient expression using the CPMV-HT system.. Enzyme assays confirmed that the plant-produced hGL was active. The figure below shows results of an assay conducted to detect the presence of lipase activity in clarified leaf extracts (performed at pH 4.0 using MU-oleate as a substrate). It was seen that extracts from leaves infiltrated with either the wt-hGL or hGL-His pEAQ-HT-based constructs had the ability to release the MU moiety from the MU-oleate substrate, demonstrating that the plant-produced lipase was active. ...
Although HL and EL display overlapping catalytic functions and lipoprotein substrate specificities, important physiological distinctions exist between these lipases. The HL/EL-dko mice share some similarities with the individual knockout mice, but they also have some remarkable differences. The greatly increased HDL in these mice improves cholesterol efflux capacity but does not increase RCT because of reduced whole-body uptake of HDL-derived cholesterol. Our study also uncovered unexpected redundant roles for both HL and EL in the metabolism of LpBs, such that a combined deficiency of these lipases results in an accumulation of sLDL.. The HL/EL-dko mice are viable, which is in contrast to the LPL-ko mice, which die as neonates.37,38 However, our data suggest that both HL and EL share overlapping roles in reproduction and survival. Wade et al39 previously addressed the impaired reproduction ability of HL-ko mice. Litter sizes for HL-ko mice in our study exactly match those of previous reported ...
Marine algae rich in n-3 PUFA, being a natural and readily available resource, could be an alternative to fish oil derived n-3 PUFA; therefore, it could be of immense potentiality in nutraceutical and pharmaceutical industries. This highlights the sustainable benefits of algae and the many potential gains in creating algal bio-factories. In recent years, the use of lipase as biocatalysts had drawn considerable attention. Lipase is an enzyme that hydrolyzes lipids, the ester bonds in triglycerides, to form fatty acids and glycerol. Among the lipases assayed, the enzyme from the yeast Candida cylindracea is of special interest, as these are proved to be a nonspecific catalyst for many (commercially) interesting reactions such as the modification of oils and fats, reactions in organic solvents, and resolution of racemic mixtures. Hence, the enrichment of microalgae using biolipase from the source Candida cylindracea is of particular attention. Lipase action of Candida cylindracea is investigated as a
DESCRIPTION (provided by applicant): There is mounting evidence indicating that endothelial lipase (EL) plays a key role in the regulation of circulating cholesterol levels by modulating high-density lipoprotein-cholesterol (HDL-C) levels and hence reverse cholesterol transport. Therefore, EL provides a promising target for therapeutic intervention to combat atherosclerosis. However, identification of small molecule inhibitors has proved difficult both in providing agents with adequate inhibitory activity as well as adequate specificity for the EL enzymatic site compared to lipoprotein (LPL) and hepatic (HL) lipases. Antibodies, in contrast, can provide the necessary specificity and blocking activity. The goals of this project are to utilize our novel yeast display platform to screen for monoclonal human antibody clones that display antibodies that bind human EL, screen the isolated clones for antibodies that block El activity, and test the selected antibody for efficacy at modulating HDL-C in ...
As lipases (E.C. 3.1.1.3) são um grupo de enzimas capazes de catalisar a hidrólise da ligação éster de triacilgliceróis, gerando ácidos graxos livres e glicerol. As lipases microbianas são muito utilizadas nas aplicações industriais nas áreas de alimentos, síntese orgânica e farmacêutica. Neste trabalho, visando à produção de lipase a partir da levedura Candida rugosa foram utilizados meios de cultura alternativos compostos por melaço, milhocina e águas russas. As fermentações foram conduzidas em agitador rotatório a 30 °C e 170 rpm. Testaram-se quatro meios contendo diferentes combinações dos resíduos acima mencionados. O meio contendo melaço 10 g/L, milhocina 4 g/L e águas russas 1,0 %(v/v) foi o que propiciou a produção de enzima com maior atividade intracelular 269 ± 10 U/L frente ao substrato pNFL (p-nitrofenil laurato). Esses resultados demonstram que o referido meio alternativo contendo resíduos agroindustriais é adequado para a produção de lipase ...
These above specific chemical transformations performed by lipases, has turned them increasingly popular in food, detergent, cosmetic, organic synthesis, and pharmaceutical industries 3. Lipases are ubiquitous in nature and are expressed by several microorganisms, namely bacteria, yeasts and fungi, as well as animals and plants 1, 3, 4. Those of microbial origin, represent the most widely used enzymes in biotechnological applications and organic chemistry 5, 6. There are two types of lipases, accordingly to their cell distribution: (i) extracellular lipases, which are secreted into culture broth and (ii) intracellular or whole cell lipases, which located either inside the cell or in the cell-wall 7. Due to their easy recovery, extracellular enzymes have been extensively studied and commercialized comparing to intracellular 1, 3, 4. On the other hand, whole-cell biocatalysts have several advantages on an industrial point of view when compared to the free extracellular enzymes, because as ...
A novel chemoenzymatic strategy for the synthesis of enantiomerically pure secondary alcohols with sterically similar substituents is described. The key step is the kinetic lipase-catalyzed resolution of racemic mixtures of substituted propargylic alcohols. The efficiency of this new approach was tested in the preparation of the corresponding enantiomers of 1,11-hexadecandiol derivatives ((R)-5 and (S)-5). Two strategies were tested. In the first one, the racemic intermediate 1-octyn-3-ol (1) was resolved enzymatically and then elongated with 1-bromo-9,11-dioxadodecane. Alternatively, the racemic 1 can be elongated to the corresponding racemic 17,19-dioxa-7-eicosyn-6-ol (3) first and then resolved biocatalytically. Twelve commercially available lipases were screened for the kinetic resolution of these intermediates. Among them, Candida antarctica lipase (CAL-B) and Humicola lanuginosa lipase (HLL) were the best biocatalysts for the resolution of 1 (S enantiomer 90% ee, E = 35), and 3 (R ...
[162 Pages Report] The microbial lipase market was valued at USD 400.6 Million in 2017 and is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018, in terms of value.
PNPLA7 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 7 (PNPLA7), transcript variant 1 - 10 µg - OriGene - cdna clones
TY - JOUR. T1 - Enzymatic polymerization catalyzed by surfactant-coated lipases in organic media. AU - Noda, Sadafumi. AU - Kamiya, Noriho. AU - Goto, Masahiro. AU - Nakashio, Fumiyuki. PY - 1997/1/1. Y1 - 1997/1/1. N2 - Structural ring-opening of lactones driven by enzymatic polymerization has been performed using low concentration dosages of surfactant-coated lipases in organic media. By comparison, enzymatic polymerization rate with coated lipase proceeded at a rate 100-fold better than native powder. Similarly a higher polymeric molecular weight (21,300), narrow dispersity (Mw/Mn = 1.9) and better conversion (100%) were obtained following polyesterification tests with surfactant-coated lipase.. AB - Structural ring-opening of lactones driven by enzymatic polymerization has been performed using low concentration dosages of surfactant-coated lipases in organic media. By comparison, enzymatic polymerization rate with coated lipase proceeded at a rate 100-fold better than native powder. ...
39] Martins A.B., da Silva A.M., Scheina M.F., Garcia-Galan C., AyubM.A.Z., Fernandez-Lafuente R., Rodrigues R.C., Comparisonof the performance of commercial immobilized lipases in thesynthesis of different flavor esters, J. Mol. Catal. B: Enzymatic,2014, 105, 18-25.[WoS][Crossref ...
TY - CONF. T1 - Characterization of in silico identified proteins with similar katalytic domains to known lipases/esterases. AU - Buchebner, Marlene. AU - Becker, Tatjana. AU - Levak-Frank, Sanja. AU - Kostner, Gerhard. AU - Kratky, Dagmar. PY - 2005/5/6. Y1 - 2005/5/6. M3 - (Old data) Lecture or Presentation. ER - ...