TY - JOUR. T1 - Effect of glutathione depletion on Leydig cell steroidogenesis in young and old Brown Norway rats. AU - Chen, Haolin. AU - Pechenino, Angela S.. AU - Liu, June. AU - Beattie, Matthew C.. AU - Brown, Terry R.. AU - Zirkin, Barry R.. PY - 2008/5. Y1 - 2008/5. N2 - Changes in the oxidant/antioxidant environment of aging Leydig cells have been shown to be correlated with the reduced ability of these cells to produce testosterone. With this in mind, we hypothesized that the experimental depletion of glutathione (GSH), an abundant Leydig cell intracellular antioxidant, might result in reduced testosterone production. Incubation of Leydig cells isolated from the testes of adult Brown Norway rats with buthionine sulfoximine (BSO) reduced GSH content by more than 70% and testosterone production by about 40%. The antioxidants vitamin E, N-tertbutyl-α-phenylnitrone and Trolox countered BSOs effect on steroidogenesis but not on GSH depletion. Together, BSO and glutathione ethyl ester ...
TY - JOUR. T1 - Severe and mild neonatal hypothyroidism mediate opposite effects on leydig cells of rats. AU - Cristovaão, Fatima C.. AU - Bisi, Helio. AU - Mendoncça, Berenice B.. AU - Bianco, Antonio C.. AU - Bloise, Walter. PY - 2002/1/1. Y1 - 2002/1/1. N2 - The effects of neonatal hypothyroidism on the number of Leydig cells were studied in neonatal Wistar rats. Moderate or severe hypothyroidism were induced during neonatal life by giving different amounts of methimazole (MMI; 0.05% or 0.1%) in the drinking water of pregnant and lactating dams. Rats were sacrificed on day 21 of postnatal life. Severely hypothyroid rats had approximately 45-fold higher serum thyrotropin (TSH) values and demonstrated approximately a 65% decrease in testes weight (p , 0.05) and the number of Leydig cells. However, in moderately hypothyroid rats, in which serum TSH was only approximately 16-fold higher, testicular weight was normal and the number of Leydig cells almost doubled (p , 0.01). There were no ...
STUDY QUESTION: Is the Leydig cell function of young European men associated with semen quality?. SUMMARY ANSWER: Compensated reduction in Leydig cell function, defined as increased LH concentration combined with adequate testosterone production is associated with lower semen quality.. WHAT IS ALREADY KNOWN: Semen quality of young European men shows a heterogeneous pattern. Many have sperm counts below and in the lower WHO reference where there nevertheless is a significant risk of subfecundity. Little is known about differences in Leydig cell function between men with semen quality below and within the WHO reference range.. STUDY DESIGN, SIZE AND DURATION: A coordinated, cross-sectional population-based study of 8182 men undertaken in 1996-2010.. PARTICIPANTS, SETTING AND METHOD: Young men (median age 19.1 years) were investigated in centres in Denmark, Estonia, Finland, Germany Latvia, Lithuania, and Spain. The men originated from the general populations, all were young, almost all were ...
TY - JOUR. T1 - Immunoregulatory activity in adult rat testicular interstitial fluid: relationship with intratesticular CD8+ lymphocytes following treatment with ethane dimethane sulfonate and testosterone implants. AU - Hedger, Mark. AU - Wang, Jian. AU - Lan, Hui Y. AU - Atkins, Robert C. AU - Wreford, Nigel G. PY - 1998. Y1 - 1998. M3 - Article. SP - 935. EP - 942. JO - Biology of Reproduction. JF - Biology of Reproduction. SN - 0006-3363. ER - ...
Patients with the testicular dysgenesis syndrome, that comprises a variable spectrum of clinical manifestations, such as infertility, cryptorchidism, hypospadias, impaired spermatogenesis and testicular germ cell neoplasms, often develop alterations in the Leydig cell compartment. These alterations range from abnormal localization and clustering to hyperplasia or tumorous formation.. Leydig cell tumors (LCTs), although uncommon in the general population, are the most frequent non-germ cell testicular neoplasms, and their incidence has been reported increasingly growing, especially in infertile patients. Given that the focal areas of Leydig cell hyperplasia are nowadays easily detectable at ultrasonography of the testis (US), as small non-palpable hypoechoic micro-nodules that can show internal vascularization, their finding create a diagnostic challenge versus low-stage malignant germ cell tumors.. Patients with testicular dysgenesis syndrome in general exhibit an elevation of ...
Patients with the testicular dysgenesis syndrome, that comprises a variable spectrum of clinical manifestations, such as infertility, cryptorchidism, hypospadias, impaired spermatogenesis and testicular germ cell neoplasms, often develop alterations in the Leydig cell compartment. These alterations range from abnormal localization and clustering to hyperplasia or tumorous formation.. Leydig cell tumors (LCTs), although uncommon in the general population, are the most frequent non-germ cell testicular neoplasms, and their incidence has been reported increasingly growing, especially in infertile patients. Given that the focal areas of Leydig cell hyperplasia are nowadays easily detectable at ultrasonography of the testis (US), as small non-palpable hypoechoic micro-nodules that can show internal vascularization, their finding create a diagnostic challenge versus low-stage malignant germ cell tumors.. Patients with testicular dysgenesis syndrome in general exhibit an elevation of ...
TY - JOUR. T1 - Specific amino acids moderate the effects of Ni2+ on the testosterone production of mouse Leydig cells in vitro. AU - Forgács, Zsolt. AU - Némethy, Zsolt. AU - Révész, Csaba. AU - Lázár, Péter. PY - 2001/3/9. Y1 - 2001/3/9. N2 - The purpose of this investigation was to study the effectiveness of two nickel-binding amino acids, histidine (His) and cysteine (Cys), to prevent the inhibitory action of Ni2+ on testosterone (T) production by mouse primary Leydig cell culture. The maximal human chorionic gonadotropin (hCG)-stimulated T response was measured by radioimmunoassay (RIA) in the culture media. Three types of experiments were performed. In a concentration-response study, Ni2+ (62.5 to 1000 μM) was added to the cells simultaneously with equimolar or twice the equimolar concentrations of His or Cys and the cultures were maintained for 48 h. Nickel-induced reduction in T production was completely prevented by equimolar concentrations of His at Ni2+ concentrations of 125, ...
Results of Western blot analysis carried out with an interstitial cell extract from male guinea pig and ovarian extract from immature female rats administered equine chorionic gonadotropin (eCG) provide supportive evidence to our earlier suggestion that an 8-kDa peptide is involved in acquisition of steroidogenic capacity by the rat Leydig cells. It was found that though the signal was observed in other tissues such as liver, kidney and lung which do not produce gonadal hormones, the peptide was modulated only by lutenizing hormone (LH) in the rat Leydig cells.. ...
Supplementary MaterialsCurley et al hAd-PSCs Supplemetary Info 41598_2019_50855_MOESM1_ESM. after transplantation suggesting this effect is probable mediated paracrine systems during the preliminary levels of regeneration; either by getting together with regenerating LCs straight, or through indirect connections with trophic macrophages. enlargement/manipulation of stem cells populations stay a significant problem. The identification and behaviour from the stem cells that provide rise to testosterone-producing Leydig cells inside the testicular interstitium continues to be a location of intense analysis - particularly with regards to harnessing their regenerative properties instead of exogenous androgen substitute. Stem Leydig cells have already been prospectively isolated from rodent and individual testes and thoroughly characterised both and in transplantation versions11C14. Although these research have got improved our knowledge of stem Leydig cell differentiation considerably, removal of ...
The 1980s heralded the discovery and identification of extra-pituitary sources of the neurohypophysial hormone oxytocin in non-neural tissues of several animal species. The presence, location and biosynthesis of significant amounts of oxytocin in the ovarian corpus luteum was followed by the immunocytochemical demonstration of an oxytocin-like peptide in the testicular interstitial cells. Leydig cells, which comprise up to 80% of the testicular intertubular cell population, are known to synthesize testosterone in situ. Indirect evidence indicated that an oxytocin-like peptide was also present in Leydig cells.. The question arose whether this peptide was synthesized de novo by Leydig cells or was taken up and stored by the cells following biosynthesis at some other intra- and/or extra-gonadal source(s). Since luteinizing hormone (LH) and ascorbate are known to augment the production of oxytocin in ovarian granulosa cells, varying concentrations of these two stimulants were used to monitor the ...
The glycoprotein hormones (LH, hCG, FSH, and TSH) have a common 92-amino acid alpha-subunit which is noncovalently linked to a hormone-specific beta-subunit. Synthetic peptides of the alpha-subunit have been shown to inhibit binding of [125I]iodo-hCG to rat ovarian membrane and [125I]iodo-TSH to human thyroid membrane preparations. Synthetic overlapping peptides of the alpha-subunit of hCG were prepared by solid phase techniques and tested in a standard in vitro rat Leydig cell bioassay. Three regions in the alpha-subunit (alpha 1-15, alpha 30-45, and alpha 71-85) were found to stimulate testosterone production. All three regions correlate with inhibition of hCG binding to ovarian receptors, but subtle differences exist between the binding sites and effector sites. These data indicate that the glycoprotein alpha-subunit has intrinsic bioactivity.
Rivenson, A; Moroson, H; and Madden, R, Receptors on rat lymphocytes for mouse leydig cells. Abstr. (1978). Subject Strain Bibliography 1978. 364 ...
Coulson, M, Gibson, GG, Plant, N, Hammond, T and Graham, M (2002) The role of a liver-testis axis in the development of Leydig cell hyperplasia ...
Isolation, Purification, and Culture of Adult Leydig Cells For isolation and purification of adult Leydig cells we used a previously described method . In
We examined in detail the differentiation of Sertoli, fetal Leydig and germ cells in XX transgenic fetal gonads at 14.5 dpc. We first performed immunofluorescence for AMH, a marker of Sertoli cell differentiation and a direct transcriptional target of SOX9 (De Santa Barbara et al., 1998), and for HSD3β, a steroidogenic enzyme expressed by fetal Leydig cells. The presence of cells expressing AMH or HSD3β confirmed that both Sertoli and fetal Leydig cells had formed in the sex-reversed XX Dmrt1 transgenic testes, as in wild-type XY testes but not XX ovaries (Fig. 2A-D). These results were confirmed by qRT-PCR (Fig. 2I,O).. We next assayed the expression of additional Sertoli and fetal Leydig cell marker genes using qRT-PCR. Genes involved in the differentiation of Sertoli (Sox8, Sox10, Ptgds and Dhh) and fetal Leydig (Star, Cyp11a1, Insl3 and Cyp17a1) cells were consistently upregulated in XX transgenic testes, as compared with wild-type ovaries (Fig. 2J-N,P; supplementary material Fig. S2A,B). ...
Pdgfr-alpha mediates testis cord organization and fetal Leydig cell development in the XY gonad.: During testis development, the rapid morphological changes ini
The studies proposed in this MERIT extension applicafion address the molecular mechanisms by which age-induced changes in the microenvironment of Leydig cells,...
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Supplementary Materials Supplementary Shape 1. the hypothalamicCpituitaryCgonadal (HPG) axis. Leydig stem cell (LSC) transplantation could give a new technique for dealing with testosterone insufficiency, but medical translatability of injecting stem cells Rabbit Polyclonal to TNF14 in the testis isnt feasible. Right here, we explore the feasibility of subcutaneously autografting LSCs in conjunction with Sertoli and myoid cells to improve testosterone. We also researched if the grafted LSCs could be regulated from the HPG axis and the molecular mechanism behind this regulation. LSCs were isolated from the testes of 12\week\old C57BL/6 mice, and subcutaneously autografted in combination with Sertoli cells and myoid cells. We found that LSCs alone were incapable of self\renewal and differentiation. However, in combination with Sertoli cells and myoid cells, LSCs underwent self\renewal as well as differentiation into mature Leydig cells. As a result, the recipient mice that received the LSC ...
Hops is best known for its use in beer. The majority of physicians and men overlook its potent chemicals and do not realize that beer itself can significantly alter the male androgen levels. German beer makers noticed long ago that the young women who picked hops in the fields commonly experienced early menstrual periods. Eventually, researchers discovered the reason - hops is perhaps one of the most powerfully estrogenic plants on Earth. Just 100 grams of hops (about 3.5 ounces) contains anywhere from thirty thousand to three hundred thousand IUs of estrogen, depending on the type of hops. Most of it is the very potent estrogen estradiol. Estradiol, as it is taken into the male body, causes a direct lowering of testosterone levels in the testes and an increase in SHBG levels, which then binds up even more free testosterone in the bloodstream. The estradiol in hops has also been found to directly interfere with the ability of the testes Leydig cells to produce testosterone. The presence of this ...
Sigma-Aldrich offers abstracts and full-text articles by [Shuqiang Liu, Dan Wang, Junwen Zhang, Dongzhi Zhang, Meng Gong, Chao Wang, Ning Wei, Wenhua Liu, Yongqi Wang, Chongxue Zhao, Yaxiong Cui, Defu Hu].
The most important hormone involved in controlling sperm production is a steroid called testosterone. This is produced in the testis itself, by the Leydig cells (see Figure 12a). The testosterone is released from the Leydig cells between the tubules, and taken up by the neighbouring Sertoli cells. The Leydig cells are stimulated to make testosterone by two other hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which are both produced by the pituitary gland and ...
Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Despite its essential roles in male reproductive and bone health, very little is known regarding its transcriptional regulation in Leydig cells. To date, few transcription factors have been shown to activate INSL3 promoter activity: the nuclear receptors AR, NUR77, COUP-TFII and SF1. To identify additional regulators, we have isolated and performed a detailed analysis of a 1.1 kb human INSL3 promoter fragment. Through 5 progressive deletions and site-directed mutagenesis, we have mapped a 10 bp element responsible for about 80% of INSL3 promoter activity in Leydig cells. This element is identical to the CPE element of the placental-specific glycoprotein-5 (PSG5) promoter that is recognized by the developmental regulator Krüppel-like factor 6 (KLF6). Using PCR and western blotting, we found that KLF6 is expressed in several Leydig and Sertoli cell lines. ...
205: The Neuroendocrine Leydig Cells and their Stem Cell Progenitors, the Pericytes (Advances in Anatomy, Embryology and Cell Biology) by JP David at AbeBooks.co.uk - ISBN 10: 3642005128 - ISBN 13: 9783642005121 - Springer - 2009 - Softcover
The human chorionic gonadotropin (hCG) test represents a key step in assessing Leydig cell function in prepubertal males, but differences in terms of hCG doses, number of injections, and sequence of blood drawing are published in the literature, showing poor standardization. The few available data in healthy boys are summarized here. A recombinant hCG (rhCG) formulation might permit overcoming some controversies as well as avoid the potential biological risk related to the injection of extractive hormones. Studies in humans are scarce, but they indicate that 250 µg rhCG in a single dose may represent a useful scheme for the dynamic evaluation of Leydig cell function in children as well as in adults ...
HCG or human chorionic gonadotropin is a drug that is used usually by women to induce ovulation. It signals the body to produce a cascade to produce eggs from the ovaries. In males this same drug can be used to signal the testes to produce testosterone by signaling the leydig cells to produce testosterone. HCG is highly effective since it has direct action on the testicles.. HOW IS HCG USED FOR STEROIDS. HCG comes in a dry powder that is in a vial. Bacteriostatic water is used to reconstitute the powder and then it is drawn out with an insulin needle. Yes, that means you need to shoot HCG in order to use it. Many people find this distasteful, but there is no other way to take HCG and have it be effective. The injections are typically done subcutaneous in the belly or in the fat of the arm.. The typical dose of HCG is about 1000iu per day but some take as high as 1500iu in a day. It is also done for five days on and two days off to avoid the burn out syndrome where the leydig cells lose ...
Foreword Alan Decherney; 1. Anatomy and embryology of the male reproductive tract and gonadal development Jane M. Lewis and William E. Kaplan; 2. Male hypothalamic-pituitary-gonadal axis Ettore Caroppo; 3. Leydig cell development and function Barry R. Zirkin, Vassilios Papadopoulos and Matthew P. Hardy; 4. Sertoli cell: morphology, function and regulation Joseph P. Alukal and Dolores J. Lamb; 5. Spermatogenesis in the adult Joseph P. Alukal, Dolores J. Lamb, Craig S. Niederberger and Antoine Makhlouf; 6. The epididymis and accessory sex organs Terry T. Turner; 7. An overview on the molecular mechanisms involved in human fertilization Monica H. Vazquez-Levin and Clara Isabel Marin-Briggiler; 8. New concepts in the genetics of male reproduction and infertility Paul J. Turek; 9. Erection, emission, and ejaculation: mechanisms of control Alexander Műller and John P. Mulhall; 10. Office evaluation of the subfertile male Mark Sigman, Larry I. Lipshultz and Stuart S. Howards; 11. Evaluation of sperm ...
It is hormone therapy and will increase the incidence logic evidence demonstrating an association with blad- metabolized and is back in anatomic position with failure does how viagra work no to respond to dopamine agonists; various colors besides white. Dupilumab is a 19-item, oughly validated and should not be the cause, if possible. However, it is a liposarcoma nucleus, few or no effect on the aggressiveness ing and frequent chalkstick fractures associated with shorter and less chloremic acidosis. Additional supportive therapies may be therapeutic. Anemia of hypoalbuminemia is thought to enhance esophageal emptying. It is difficult because results of these studies ing tumors (testicular leydig cell failure in cant due to gram-positive or gram-negative 4. Specific antibody deficiency that is almost invariably anemic at presentation. Fevers and lymphoid tissue tomy and radiation or surgery 1.4 presents with (from bees), vitamin e, for most of circulating red blood cells, is recognized by echo- ...
Antonietta cipro - Certain patients may receive combined chemotherapy with 25,843 long-term survivors of testicular leydig cell and nongerm cell tumors ings on examination. In ckd. Cancer 1972; markers, based on the peritoneum and preperitoneal fat.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The origin, development, and characteristics of two types of testicular macrophage have been described. To elucidate the nature of these immune cells, the researchers used a novel cell tracing method. Their findings are of fundamental importance. They may help understand certain kinds of infertility in men and find new treatments for them. Read more ...
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Buckwalter JA, Mankin HJ: available download organic structures design study and copolymer. Fuchs JR, Terada S, Ochoa ER, Vacanti JP, Fauza DO: s evaluation engineering: In utero theoretical bone in an maintenant monitoring. actual download organic without fluid data. Machluf M, Orsola A, Boorjian S, Kershen R, Atala A: Microencapsulation of Leydig Materials: A support for research modulation. Tai J, Johnson HW, Tze WJ: crucial download organic structures design applications in optical and electronic devices of Leydig developments in presented s Sanctions. Dam JH, Teerds KJ, Rommerts FF: health and organisational value of dynamic images of joint Leydig donors. Tai IT, Sun AM: download organic structures of future universities: A useful s element for protein-2 osteosarcoma. Nomi M, Atala A, Coppi PD, Soker S: increases of class for subsea Australia. Otte, Hadi Hezaveh and Justin J. Waddington Rachel J ISBN: 9781118741108 with BooksBob. three-year and Total Prospects visibility. ISBN: ...
1,3-Dichloro-2-propanol (1,3-DCP) is a well-known food processing contaminant that has been shown to impede male reproductive function. However, its mechanism of action remains elusive. In this study, the effects of 1,3-DCP on progesterone production were investigated using the R2C Leydig cell model. 1,3-DCP significantly reduced cell viability from 7.48% to 97.4% at doses comprised between 0.5 an ...
Leydig cells synthesize and secrete testosterone, and are regulated by Sertoli cells. These two cell types may work together to regulate testicular androgen production. Studies have shown that Leydig cell androgen synthesis can be dramatically enhanced by Sertoli cells in the presence of melatonin, which can regulate the secretory function of Leydig and Sertoli cells. However, the molecular mechanism of melatonin-regulated Leydig cell androgen production via Sertoli cells remains unclear. Here, we found that 10^−7 M melatonin increased testosterone production in co-cultured Leydig and Sertoli cells isolated from sheep. Melatonin increased the expression of stem cell factor and insulin-like growth factor-1 and decreased estrogen synthesis in Sertoli cells. Melatonin promoted insulin-like growth factor-1 and decreased estrogen content via the membrane melatonin receptor 1. It also enhanced stem cell factor expression via the retinoic acid receptor-related orphan receptor alpha. Addition of ...
Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, however, whether such early gestational and/or lactational exposure can influence the later adult-type Leydig cell phenotype. In this study, Sprague-Dawley rats were exposed to dibutyl phthalate (DBP; from gestational day (GD) 14.5 to postnatal day (PND) 6) or diethylstilbestrol (DES; from GD14.5 to GD16.5) during a short gestational/lactational window, and male offspring subsequently analysed for various postnatal testicular parameters. All offspring remained in good health throughout the study. Maternal xenobiotic treatment appeared to modify specific Leydig cell gene expression in male offspring, particularly during the dynamic phase of mid-puberty, with serum INSL3 concentrations showing that these compounds led to a faster attainment of peak values, and a modest acceleration of ...
TY - JOUR. T1 - Selective GPER activation decreases proliferation and activates apoptosis in tumor Leydig cells. AU - Chimento, A.. AU - Casaburi, I.. AU - Bartucci, M.. AU - Patrizii, M.. AU - Dattilo, R.. AU - Avena, P.. AU - Andò, S.. AU - Pezzi, V.. AU - Sirianni, R.. PY - 2013/8. Y1 - 2013/8. N2 - We have previously shown that estrogens binding to estrogen receptor (ER) a increase proliferation of Leydig tumor cells. Estrogens can also bind to G protein-coupled ER (GPER) and activation of this receptor can either increase or decrease cell proliferation of several tumor types. The aim of this study was to investigate GPER expression in R2C rat tumor Leydig cells, evaluate effects of its activation on Leydig tumor cell proliferation and define the molecular mechanisms triggered in response to its activation. R2C cells express GPER and its activation, using the specific ligand G-1, is associated with decreased cell proliferation and initiation of apoptosis. Apoptosis after G-1 treatment was ...
The aim of the present study was to compare the transphosphatidylation activity of phospholipase D (PLD) under different substrate labeling conditions, in order to investigate whether PLD in rat Leydig cells exhibited any substrate preferences for the alkyl- or acyl-form of phosphatidylcholine (PtdCho). The [H] phosphatidylethanol formation in response to 4ß-phorbol 12-myristate 13-acetate (PMA), sphingosine, or Ca-ionophore A23187, was lower when Leydig cells were labeled with 1-O-[H]alkyl lysoPtdCho compared with the responses when [H]myristic acid was employed. In contrast, the results for the receptor agonists (vasopressin, bradykinin, and lysophosphatidic acid), using the two labels, showed mole consistency. Thus, the PLD-activity induced by PMA, sphingosine, or A23187 has a more selective substrate range (i.e. mainly acyl-linked PtdCho) than the PLD-activity stimulated via a receptor. Our data suggests the existence of PLD isozymes that differ with respect to substrate specificity and ...
Leydig cell adenoma Digital cases HPC:119 : Leydig cell tumor. JRC:18782 : Testis intestitial cell tumor Images Leydig cell tumor (...)
Inhibin, beta B, also known as INHBB, is a protein which in humans is encoded by the INHBB gene. INHBB is a subunit of both activin and inhibin, two closely related glycoproteins with opposing biological effects. Inhibins are heterodimeric glycoproteins composed of an α subunit (INHA) and one of two homologous, but distinct, β subunits (βA or βB, this protein). mRNA for the two subunits has been demonstrated in the testes of adult rats. Inhibin can bind specifically to testicular interstitial cells throughout development and may be an important regulator of Leydig cell testosterone production or interstitial cell function. The inhibin beta B subunit joins the α subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as ...
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Fetal male sexual differentiation is driven by two testicular hormones: testosterone (synthesized by interstitial Leydig cells) and antimullerian hormone (AMH; produced by Sertoli cells present in the seminiferous tubules). Intersex states result either from gonadal dysgenesis, in which both Leydig and Sertoli cell populations are affected, or from impaired secretion or action of either testosterone or AMH. Until now, only Leydig cell function has been assessed in children with ambiguous genitalia, by means of testosterone assay. ...
D-Aspartic acid (D-Asp) and nitric oxide (NO) are two biologically active molecules playing important functions as neurotransmitters and neuromodulators of nerve impulse and as regulators of hormone production by endocrine organs. We studied the occurrence of D-Asp and NO as well as their effects on testosterone synthesis in the testis of boar. This model was chosen for our investigations because it contains more Leydig cells than other mammals. Indirect immunofluorescence applied to cryostat sections was used to evaluate the co-localization of D-Asp and of the enzyme nitric oxide synthase (NOS) in the same Leydig cells. D-Asp and NOS often co-existed in the same Leydig cells and were found, separately, in many other testicular cytotypes. D-Asp level was dosed by an enzymatic method performed on boar testis extracts and was 40+/-3.6 nmol/g of fresh tissue. NO measurement was carried out using a biochemical method by NOS activity determination and expressed as quantity of nitrites produced: it ...
2008). Mastermindlike domain-containing 1 (MAMLD1 or CXorf6) transactivates the Hes3 promoter, augments testosterone production, and contains the SF1 target sequence ...
학술회의] 민철기, Hae-Kwon Kim, Jae-Ho Lee, Yun-Jin Jung, Kyoo Wan Choi, Seung-Jae Lee, Sung Eun Lee, Hwa Lee Ryu, Ju-Hee Lee, Inhibitory action of mibefradil on steroidogenesis in mouse leydig cells: involvement of Ca+2 entry through T-type Ca+2 channels in steroidogenesis , ESHRE 21st Annual Meeting , Vol.21 , pp.121 -121 (Jun, 2005) ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Health Aspects of Cannabis Page 18. and live or killed gram negative bacteria. Infect. Immun. 17: 325 329, 1977.. 20. Braunstein, G.D., Buster, J.E., Soares, J.R., and Gross, S.J.: Pregnancy hormone concentrations in marijuana users. Life Sci. 33: 195 199, 1983.. 21. Burstein, S., Hunter, S.a., and Sedor, C.:Further studies in the inhibition of Leydig cell testosterone synthesis by delta 1 tetrahydrocannabinol. Biochem. Pharmacol. 29: 2152 2154, 1980.. 22. Campbell, A.M.G., Evans, M., Thompson, J.L.G., and Williams, M.R.: Cerebral atrophy in young cannabis smokers. Lancet 2: 1219 1224, 1971.. 23. Carlen, P.L., Wortzman, G., Holgate, R.C., Wilkerson, D.A., and Rankin, R.G.: Reversible cerebral atrophy in recently abstinent chronic alcoholics measured by computed tomography scans. Science (Wash. DC) 200: 1076-1078, 1978.. 24. Carlen, A.S., and Trupin, E.W.: The effect of long term chronic marijuana use on neuropsychological functioning. Int. J. Addict. 1275: 617-624, 1977.. 25. Carlini, E.A., and ...
The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) was shown to mimic luteinizing hormone (LH; lutropin) in causing desensitization of LH-mediated cyclic AMP production in tumour Leydig cells. However, there were differences between LH- and TPA-induced desensitization: (1) TPA induced a more rapid effect than LH; (2) adenosine did not inhibit TPA-induced desensitization, whereas it completely inhibited the LH-induced desensitization; (3) adenylate cyclase activity in plasma membranes from TPA-desensitized cells was not decreased, whereas similar preparations from LH-desensitized cells lost their response to LH and to LH plus guanosine 5′-[beta gamma-imido]triphosphate; TPA-, but not LH-, treated cells had a decreased capacity to respond to cholera toxin and forskolin. These results indicate that LH and phorbol esters induce desensitization of adenylate cyclase in rat tumour Leydig cells by different mechanisms. ...
Chrysin - If youre not afraid of hefty doses, you might be able to up your testosterone level by taking the flavonoid chrysin [structural formula shown here]. An animal study published by researchers at the University of Inonu, Turkey in Andrologia suggests this may be the case. Chrysin was a hip supplement in the nineties, after test-tube studies had shown that it sabotages the enzyme aromatase. Supplements manufacturers seeking to boost testosterone concentration in the body added chrysin to their combinations - until it became clear that enzymes remove chyrsin so quickly from the body that supplements containing a few score milligrams hardly have any effect on testosterone levels.. Meanwhile chrysin and its analogues have started to attract attention again. Studies have now shown that substances resembling chrysin boost testosterone synthesis. Apigenin in parsley is one of these. Isorhamnetin in onions is another.. These recent studies were also done in test tubes, which is why the Turkish ...
Corticotropin-releasing hormone (CRH), the principal neuropeptide regulator of pituitary ACTH secretion, is also produced at peripheral inflammatory sites, where it acts as a proinflammatory cytokine, and by the Leydig cell of the testis, where it exerts autocrine inhibition of testosterone biosynthesis. Because key ovarian functions, such as ovulation and luteolysis, represent aseptic inflammatory responses, and because the theca cell is the functional equivalent of the Leydig cell, we explored the CRH presence in the ovary, first, by specific CRH immunohistochemistry of adult cycling female Sprague-Dawley rat ovaries. We detected cytoplasmic immunoreactive CRH (IrCRH) in theca and stromal cells and in cells within the corpora lutea, at all phases of the estrous cycle. Using a specific radioimmunoassay, we measured IrCRH in extracts of rat ovaries (0.042-0.126 pmol/g wet tissue). The mobility of the ovarian IrCRH molecule was similar to that of rat/human CRH by reverse phase HPLC. To ...
A section of the left testis shows a well circumscribed but unencapsulated nodule just outside the tunica albuginea composed of medium-sized oval to polygonal cells with abundant dense granular eosinophilic cytoplasm, round to oval nuclei, stippled chromatin and no mitoses. The cells are arranged in nests and cords. There are numerous congested capillaries within the nodule (some containing fibrin) and several compressed ducts around the periphery. The testis is atrophic with markedly decreased spermatogenesis, multifocal tubular and peritubular fibrosis, moderate interstitial edema, and very few interstitial Leydig cells. The right testis contains no lesions. An immunostain for vimentin was lightly and diffusely positive.. ...
No, it is not a psychological effect, since the old guy had no idea whatsoever that I gave him damiana. Damiana is able to increase the secretion of male hormones such as testosterone, which plays a crucial role in the formation and maturation of sperm. The prostatefor the local inhabitants. They help stimulate blood flow, increase testosterone production in men and improve ejaculation problems. Digestive health support. One study on 188 college students (male and female) showed that a substantial part of the effect of testosterone on attitude to risk is mediated by abstract reasoning ability . Its primary use in … Damiana is a shrub whose leaves contain precious active ingredients: flavonoids, alkaloids, or even damianine. https://www.livestrong.com/.../118189-benefits-damiana-leaf Tongkat ali, also known as longjack, is a flowering plant that is native to places like Indonesia, Malaysia, Thailand and India.The root of the plant is boiled in water, and it is turned into a tonic to treat a ...
Moving on to the realm of certainties, glands are the organs of our bodies that produce testosterone, more specifically, they synthesize testosterone from cholesterol but more on that later. In both men and women, adrenal glands manufacture testosterone in barely noticeable amounts. At the cellular levels, the main producer of testosterone are Leydig cells, named after the anatomist who discovered them in 1850. The testicles display a large number of Leydig cells, therefore they secrete a high amount of testosterone, while the ovaries have a much smaller amount of this type of cells in their make-up.. Upon actions from the hypothalamus (the part of the central nervous system whose primary function is to control the endocrine system) through the pituitary gland, testicles begin to secrete increasingly larger amounts of testosterone during puberty. The amount remains relatively high until the man reaches his late 20s. Around 30 years of age, human males start experiencing a decrease in ...
TY - JOUR. T1 - Structural and functional analysis of the promoter region of the gene encoding mouse steroid 11β-hydroxylase. AU - Mouw, A. R.. AU - Rice, D. A.. AU - Meade, J. C.. AU - Chua, S. C.. AU - White, P. C.. AU - Schimmer, B. P.. AU - Parker, K. L.. PY - 1989/1/1. Y1 - 1989/1/1. N2 - The mouse gene encoding adrenal steroid 11β-hydroxylase (11β-OHase) has been cloned and the nucleotide sequence of its 5 end has been determined. The coding regions sequenced are homologous (75%) to the sequence of bovine 11β-OHase cDNA. The 5-flanking region of the 11β-OHase gene contains a potential cAMP response element (TGACGTGA) located 56 base pairs upstream of the transcription initiation site (position -56) and two motifs at positions -249 and -148 which are similar to an element postulated to be required for the expression of 21-hydroxylase. Transfection of mouse Y1 adrenocortical tumor cells and MA-10 testicular Leydig cells with plasmids containing the 11β-OHase promoter linked to a ...
We have previously reported that genetically increased angiotensin-converting enzyme levels, or absence of the bradykinin B2 receptor, increase kidney damage in diabetic mice. We demonstrate here that this is part of a more general phenomenon - diabetes and, to a lesser degree, absence of the B2 receptor, independently but also largely additively when combined, enhance senescence-associated phenotypes in multiple tissues. Thus, at 12 months of age, indicators of senescence (alopecia, skin atrophy, kyphosis, osteoporosis, testicular atrophy, lipofuscin accumulation in renal proximal tubule and testicular Leydig cells, and apoptosis in the testis and intestine) are virtually absent in WT mice, detectable in B2 receptor-null mice, clearly apparent in mice diabetic because of a dominant mutation (Akita) in the Ins2 gene, and most obvious in Akita diabetic plus B2 receptor-null mice. Renal expression of several genes that encode proteins associated with senescence and/or apoptosis (TGF-β1, ...
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WebMD does not present medical recommendation, analysis or remedy. LC transplantation is a perfect physiological and lengthy-performing system for the testosterone supply 16 Nevertheless, LCs account for less than ~ 2-four% of the total testicular cell population in grownup human testes 17 Furthermore, LCs are terminally differentiated cells with a restricted capacity to proliferate four , thereby limiting the efficacy of LC transplantation therapy.. Machluf, M., Orsola, A., Boorjian, S., Kershen, R. & Atala, A. Microencapsulation of leydig cells: a system for testosterone supplementation. Molenaar, R., de Rooij, D. G., Rommerts, F. F., Reuvers, P. J. & van der Molen, H. J. Specific destruction of leydig cells in mature rats after in vivo administration of ethane dimethyl sulfonate.. Most cancers is the uncontrolled development of irregular cells in the physique.These regular cells develop uncontrolled and even intrude in other surrounding tissues. After 30 day differentiation, the RT-PCR assay ...
Unfortuately I was not able to find a proper explanation of testosterone synthesis in the human body... anyone got an url for me? However, what I know
Peh, K.L., Gunasegaram, R., Loganath, A., Cheah, E., Ratnam, S.S. (1987). Testosterone synthesis by infiltrating ductal human breast carcinoma in vitro. Medical Science Research 15 (23) : 1467-1468. [email protected] Repository ...
So I finished a cycle a of tren and test a few months back, did pct and everything except libido came back to normal, which from what I understand isn
Purpose: Varicocele, most treatable pathologic condition in male infertility, exerts unfavorable effects on testicular ultrastructure via various mechanisms. In this study we aimed to demonstrate adverse effects of varicocele on both testes. ...
Testosterone Levels Articles: Get information on Testosterone Levels. Read articles and learn about all the facts related to Testosterone Levels from our health website Onlymyhealth.com.
Have you already had your tablespoon of honey, Honey? If thats what your girlfriend or wife asked you this morning, she is probably concerned about your testicular health... A group of Malaysian scientists has recently been able to show that 1.2g/kg/day (human equivalent: 0.2g/kg/day or about 1 tablespoon for an average adult man) of a off-the-shelf Malaysian Tulang honey protected the testis of rats, who had been exposed to cigarette some for 8 minutes three times per day, from damage and oxidative stress (Mohamed. 2011 ...
Os roedores formam o grupo com maior diversidade de espécies na Classe Mammalia, abrigando indivíduos de poucos gramas como o camundongo, até espécies de 90 quilos como a capivara. A despeito de sua diversidade e do elevado número de representantes, poucos se sabe sobre a biologia reprodutiva da maioria dos animais deste grupo, o que dificulta a aplicação de medidas de conservação e manejo das mesmas. Por sua diversidade e pela facilidade de serem amostrados sistematicamente, os roedores silvestres tornaram-se modelos muito úteis para investigações. Assim, objetivou-se realizar estudos do processo espermatogênico de Oligoryzomys nigripes e Akodon cursor, ambos endêmicos da Mata Atlântica, visando obter dados básicos sobre a reprodução de roedores silvestres. Fragmentos testiculares foram processados para análise morfológica, imunohistoquímica e ultraestrutural. O índice gonadossomático encontrado para O. nigripes e A. cursor foi, respectivamente, de 0,68% e 0,74% enquanto ...
Early diagnosis of testicular cancer; regulation of germ cell differentiation during testis development; genetic causes of male infertility; Klinefelter syndrome; Leydig cell function ...
Abstract: The toxic effect of various environmental contaminants such as heavy metals or industrial chemicals such as alkylphenolson male reproductive systemwasfound. 4-nonylphenol (4-NP), one of the environmental contaminants used in the manufacture of plastics and other products, is released largely into the environment. The majority of studies on NP have focused on their endocrine disrupting and potential adverse effects on reproductive system. The target of our in vitro study was to determine the potential impact of 4-nonylphenol as an endocrine disruptor on the hormonal secretion and viability of mice Leydig cells. Cells were cultured with addition of 0.04; 0.2; 1.0; 2.5; and 5.0 µg/mL of 4-nonylphenol for 44h and compared with the control. Cells destined for the determination of androstenedione production were cultured in the presence of cyclic AMP solution. Quantification of cAMP stimulated androstenedion production directly from aliquots of the medium was performed by enzyme linked ...
Some, but not all, of a series of novel pyrrolo-1,5-benzoxazepines (PBOXs) induce apoptosis as shown by cell shrinkage, chromatin condensation, and DNA fragmentation in three human cell lines, HL-60 promyelocytic, Jurkat T lymphoma, and Hut-78 s.c. lymphoma cells. This chemical selectivity, together with the lack of apoptotic activity against rat Leydig cells, argues against a general cell poisoning effect. PBOX-6, a potent member of the series, caused activation of a member of the caspase-3 family of proteases. In addition, the caspase-3-like inhibitor z-DEVD-fmk, but not the caspase-1-like inhibitor z-YVAD-fmk prevented PBOX-6-induced apoptosis, suggesting that caspase 3-like proteases are involved in the mechanism by which PBOX compounds induce apoptosis. The release of cytochromec into the cytosol in HL-60 cells in response to PBOX-6 suggests that this cellular response may be important in the mechanism by which PBOX-6 induces apoptosis. However, reactive oxygen intermediates do not play a ...
Hey :-) i am a trans guy 2,5 years on testosterone and i appreciate the presence of this community of people with the same anatomy and your will in giving advice.. I prefer to be referring to my anatomy in a gender neutral way. So, i am a person who is positive towards sex and penetration was…
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Heres what you can expect during your first year on testosterone whether youve undergone transgender cosmetic surgery or not.
Unfortunately, while HCG increases Testosterone, it increases estrogen as well(12). As you probably know, estrogen acts directly on the Leydig cells to effect changes in the activities of enzymes important for testosterone synthesis (13) and may actually be considered an important part of that negative feedback loop I mentioned earlier. In addition, an increase in circulating levels of LH have been shown to induce down-regulation of LH-receptors in both rodent studies (14), as well as in human studies (15); since HCG mimics LH, you can expect it to do the same. This LH downregulation can cause an increase in steroidogenic cholesterol (the cholesterol earmarked by your body for conversion into testosterone). (16). Thus, after the initial HCG induced surge in testosterone is over, if you have used enough to downregulate your LH-receptors and increase estrogen too much, then more steroidogenic cholesterol is available. This is telling me that less is being converted to testosterone. In fact, rodent ...
Pour les adultes enthousiastes du cannabis. Sign in to like videos, comment, and subscribe. CBD and Testosterone: Does CBD Oil Lower Testosterone CBD exerts a protective effect for testosterone in the liver; CBD does appear to slow T synthesis in the Leydig cells, however at the same time it does not lower serum testosterone levels. It seems to just slow down this production process while its in the blood stream, but then T production returns to normal when the CBD is gone. No long CBD - 10MG Cran Razz Pot Shots , Leafly About this product. HI-NESS CBD Elixirs come in 3 flavors. Theyre an exhilarating way to relax and enjoy your time. Our products are SC Lab tested and meet all requirements. Smokables - Golden Cherry CBD Hemp - Pots and Shots LLC. ...
During the month of Muslim fasting (Ramadan) many people alter their sleeping habits and stay awake most of the night In this study I looked into the effect of this alteration as well as the effect of fasting on testosterone and its circadian variation in fifty-eight (58) healthy adults. Fortysix (46) of the subjects showed alteration of the diurnal variation during the third week of fasting. Thirty-eight (38) males showed significant increase in evening testosterone level and eight (8) females showed significant decrease of morning testosterone level (P < 0.001 in both). These findings suggest that alteration in sleeping pattern during Ramadan has a significant effect on testosterone levels.
Testosterone is part of a group of hormones called androgens - the kind of hormones that regulate the development of male characteristics in humans. Special cells called Leydig cells in the testes are mainly responsible for testosterone production. What does testosterone do?
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When it comes to maximizing your strength and muscle building potential, then Zinc is of paramount importance in that respect. You see Zinc is nessesary for Vitamin A to be properly transported from the liver. Zinc is also responsible for protein synthesis, RNA, and DNA synthesis and is a primary potentiator for Testosterone production. Zinc inhibits levels of aromatase throughout the body so that natural Testosterone levels will remain high. Zinc also stimulates the Pituitary Gland to release Luteinizing Hormone which in turn causes the Testes to produce Testosterone. The best supplement we have found so far is from a Company called Natures Life which is Zinc Picolinate in a 30mg strength. The RDA is set at 15mg per day but for Weight and Strength Trainers using a high protein diet, a good start would be 30mg per day. Zinc is one of the most important Minerals nessesary for Bodybuilding fitness and health. Please remember to always consult with your Doctor before starting any new supplement ...
CL:0000017 ! spermatocyte CL:0000018 ! spermatid CL:0000019 ! sperm CL:0000023 ! oocyte CL:0000025 ! egg CL:0000026 ! nurse cell CL:0000030 ! glioblast CL:0000031 ! neuroblast CL:0000034 ! stem cell CL:0000037 ! hematopoietic stem cell CL:0000056 ! myoblast CL:0000057 ! fibroblast CL:0000062 ! osteoblast CL:0000066 ! epithelial cell CL:0000071 ! blood vessel endothelial cell CL:0000075 ! columnar/cuboidal epithelial cell CL:0000081 ! blood cell CL:0000084 ! T cell CL:0000092 ! osteoclast CL:0000094 ! granulocyte CL:0000097 ! mast cell CL:0000115 ! endothelial cell CL:0000125 ! glial cell CL:0000127 ! astrocyte CL:0000128 ! oligodendrocyte CL:0000129 ! microglial cell CL:0000134 ! mesenchymal cell CL:0000136 ! fat cell CL:0000138 ! chondrocyte CL:0000147 ! pigment cell CL:0000148 ! melanocyte CL:0000150 ! glandular epithelial cell CL:0000178 ! Leydig cell CL:0000187 ! muscle cell CL:0000188 ! skeletal muscle cell CL:0000192 ! smooth muscle cell CL:0000201 ! auditory receptor cell CL:0000202 ! ...
CL:0000017 ! spermatocyte CL:0000018 ! spermatid CL:0000019 ! sperm CL:0000023 ! oocyte CL:0000025 ! egg CL:0000026 ! nurse cell CL:0000030 ! glioblast CL:0000031 ! neuroblast CL:0000034 ! stem cell CL:0000037 ! hematopoietic stem cell CL:0000056 ! myoblast CL:0000057 ! fibroblast CL:0000062 ! osteoblast CL:0000066 ! epithelial cell CL:0000071 ! blood vessel endothelial cell CL:0000075 ! columnar/cuboidal epithelial cell CL:0000081 ! blood cell CL:0000084 ! T cell CL:0000092 ! osteoclast CL:0000094 ! granulocyte CL:0000097 ! mast cell CL:0000115 ! endothelial cell CL:0000125 ! glial cell CL:0000127 ! astrocyte CL:0000128 ! oligodendrocyte CL:0000129 ! microglial cell CL:0000134 ! mesenchymal cell CL:0000136 ! fat cell CL:0000138 ! chondrocyte CL:0000147 ! pigment cell CL:0000148 ! melanocyte CL:0000150 ! glandular epithelial cell CL:0000178 ! Leydig cell CL:0000187 ! muscle cell CL:0000188 ! skeletal muscle cell CL:0000192 ! smooth muscle cell CL:0000201 ! auditory receptor cell CL:0000202 ! ...
Summary of TRPV5 expression in human tissue. Kidney tubules, adrenal gland, macrophages of lung, stomach and cells in seminiferous ducts showed moderate to strong cytoplasmic and membranous positivity. Subset cell of gastrointestinal tract, gall bladder, follicle cells, exocrine glandular cells, Leydig cells and salivary gland showed moderate cytoplasmic positivity. Remaining normal tissues were weakly stained or negative.
Testosterone is responsible for a number of functions in the body. Its a primary androgenic hormone that is responsible for the development and growth of secondary male or masculine characteristics. It is produced in the Leydig cells of the...
the expression of AR from five age groups [postnatal 1(neonatal), 10, 25, 45 and 60 days (adult) of age] was examined using immunohistochemistry method. The results were as follows: ① There was AR expression in leydig cells in neonatal voles and the expression of AR decreased at postnatal 10 days and 25days. AR expression reached its peak at postnatal 45 days and then decreased in adults( ...
Summary of AAMDC expression in human tissue. Cytoplasmic expression in a subset of cells including Leydig cells, Purkinje cells and cells in the gastric mucosa.
Summary of SAMSN1 (HACS1, NASH1, SASH2, SH3D6B, SLy2) expression in human tissue. Cytoplasmic expression in several different tissues, most abundant in Leydig cells and immune cells.