This trial is investigating the alterations in mRNA and protein expression in human peripheral mononuclear blood cells (pmc) of schizophrenia patients treated
TY - JOUR. T1 - An ACTH Receptor on Human Mononuclear Leukocytes. AU - Smith, Eric M.. AU - Brosnan, Pat. AU - Meyer, Walter J.. AU - Blalock, J. Edwin. PY - 1987/11/12. Y1 - 1987/11/12. N2 - THE inaccessibility of many tissues makes routine investigation for receptor defects impossible. ACTH insensitivity syndrome, first described by Shepard et al.1 in 1959 and now reported in more than 63 patients,1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 is an example of this problem. Defects in adrenal ACTH receptors have been postulated because of high circulating ACTH levels and the ability of the adrenal-cortex cells to achieve steroidogenesis through an elevation of cyclic AMP.1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 In the majority of cases, the clinical presentation includes hyperpigmentation, hypoglycemia, and extremely low glucocorticoid levels with normal mineralocorticoid concentrations.2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Differences between cases suggest that the syndrome may have ...
Previous studies have indicated that the cytokine transforming growth factor beta 1 (TGF beta 1) has immunosuppressive properties and can inhibit the production of tumor necrosis factor (TNF) and Interleukin 1 (IL 1) by human peripheral blood mononuclear cells. In this study, we have examined the effects of TGF beta 1 on the production of Interleukin 6 (IL 6) by human peripheral blood mononuclear cells. Treatment with only TGF beta 1 leads to the induction of IL 6, and this was both dose- and time-dependent. The effect of TGF beta 1 was evident at the level of IL 6 mRNA, suggesting TGF beta 1-induced de novo synthesis of IL 6. Induction of IL 6 by TGF beta 1 was specific, as other cytokines made by mononuclear cells (TNF and IL 1) were not induced by TGF beta 1. Furthermore, when a panel of stimuli were compared for their ability to induce IL 1, TNF and IL 6 in the presence or absence of TGF beta 1, IL 6 levels were augmented in the presence of TGF beta 1, while the induction of IL 1 and TNF was
This is a prospective, randomized, controlled, double-blind and cross-over study. The intervention will consist in the administration of 250g of chocolate cream with or without the extract after 12 hours fasting. Blood samples will be taken at point 0, 2, 3 and 6 hours after the administration. At each time point, blood pressure and endothelial function (with a non-invasive method) will be measured ...
PRIMARY OBJECTIVES:. I. To evaluate whether we can safely administer NY-ESO-1 T cell receptor transduced autologous peripheral blood mononuclear cells (PBMCs) (up to 1x10^9 cells) along with an NY-ESO-1 dendritic cell vaccine and low dose IL-2 to patients with advanced malignancies.. II. To evaluate the feasibility of delivering two patient-specific cell therapies, the NY-ESO-1 TCR transgenic peripheral blood mononuclear cell (PBMC) and NY-ESO-1 (157-165) peptide pulsed dendritic cells (DC), within a technically challenging study design that requires other significant interventions, like a lymphodepleting conditioning regimen and post-infusion of subcutaneous low dose interleukin (IL)-2 (aldesleukin).. III. To determine the rate of objective tumor responses, by Response Evaluation Criteria in Solid Tumors (RECIST) objective response criteria.. SECONDARY OBJECTIVES:. I. To determine the persistence of NY-ESO-1 TCR-engineered cells. This will be determined by temporally analyzing peripheral blood ...
Cryopreserved Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma ...
A number of different vaccines designed to boost and harness the immune response against prostate cancer are undergoing evaluation in clinical trials. Sipuleucel-T (Provenge, Dendreon, Seattle, WA, USA) is an immunotherapy cellular product consisting of autologous peripheral blood mononuclear cells enriched for a dendritic cell fraction pulsed with PA2024, a prostatic acid phosphatase (PAP)-GM- granulocyte macrophage-colony stimulating factor (CSF) construct. Patients with asymptomatic and metastatic chemo-naive CRPC were randomized in the D9901 trial to receive sipuleucel-T (n = 82) or a placebo (n = 45) every 2 weeks for three doses (Table 1).26 The vaccine produced prostate cancer specific immune response and the median survival was 25.9 months with sipuleucel-T compared with 22.0 months for placebo (P = 0.02). At 36 months, 33% of sipuleucel-T patients were alive, compared with 11% in the placebo group. However, the PSA response rate was poor and no improvement was observed in time to ...
The mechanisms by which HIV-1 infection kills T lymphocytes are not clearly established. Apoptosis is an internally programmed cell death pathway that may regulate both T cell development and senescence, and that is characterized by cleavage of DNA at internucleosomal regions. The present experiments show that acute HIV-1 infection of MT2 lymphoblasts and activated normal peripheral blood mononuclear cells induces apoptosis. The addition of anti-gp120 neutralizing antibody, after HIV-1 infection of MT2 cells, permitted sustained high levels of viral replication, but blocked apoptosis and cell death. Apoptosis may account for the direct cytopathologic effects of HIV-1 in T cells.. ...
Gal-1 is a secreted protein that is expressed by both tumor and host cells from various organs (28). Rabinovich and colleagues had previously validated that Gal-1 plays an immunoregulatory role through T-cell apoptosis, using cultured peripheral blood mononuclear cells (29). It has also been shown that abrogating Gal-1 expression in Hs683 giloblastoma cells and B16F10 melanoma cells increased the sensitivity to chemotherapeutic agents (30, 31). The role of host Gal-1 and tumor cell Gal-1 during tumor progression is yet to be determined.. Here we show that tumor Gal-1 is more critical in promoting tumor growth and metastasis and that host Gal-1 is less essential in our model. Depleting Gal-1 in the tumor resulted in a significant tumor growth delay and aborted the development of spontaneous lung metastasis in subcutaneously implanted tumors. Tumors expressing Gal-1 grew at the same rate and had similar numbers of lung metastases regardless of the host phenotype (Gal-1 WT and null mice), ...
Our research laboratory has been focused on establishing novel cellular and animal models of human cardiovascular diseases for the purpose of elucidating causative mechanisms and identifying potential therapeutic interventions to treat those diseases. Through a close collaboration with several clinicians at Yale, we are able to obtain cells from a variety of tissues procured from healthy subjects and patients with cardiovascular diseases. These cells include dermal fibroblast cells derived from skin punch biopsies or peripheral mononuclear blood cells, which are isolated and reprogrammed into induced pluripotent stem (iPS) cells by introducing stem cell factors before being re-differentiated into functional cardiovascular cells. In this way, we have the ability to derive an unlimited amount of human cardiovascular cells for use in our investigations into the specifics of cardiovascular disease mechanisms and the discovery of potential therapeutic treatments by performing high-throughput drug ...
Verbrugge SE, Assaraf YG, Dijkmans BA, Scheffer GL, Al M, den Uyl D, Oerlemans R, Chan ET, Kirk CJ, Peters GJ, van der Heijden JW, de Gruijl TD, Scheper RJ, Jansen G. Inactivating PSMB5 Mutations and P-Glycoprotein (Multidrug Resistance-Associated Protein/ATP-Binding Cassette B1) Mediate Resistance to Proteasome Inhibitors: Ex Vivo Efficacy of (Immuno)Proteasome Inhibitors in Mononuclear Blood Cells from Patients with Rheumatoid Arthritis. J Pharmacol Exp Ther. 2012 Apr;341(1):174-82. Epub 2012 Jan 10. (3/13/12 KBj ...
Verbrugge SE, Assaraf YG, Dijkmans BA, Scheffer GL, Al M, den Uyl D, Oerlemans R, Chan ET, Kirk CJ, Peters GJ, van der Heijden JW, de Gruijl TD, Scheper RJ, Jansen G. Inactivating PSMB5 Mutations and P-Glycoprotein (Multidrug Resistance-Associated Protein/ATP-Binding Cassette B1) Mediate Resistance to Proteasome Inhibitors: Ex Vivo Efficacy of (Immuno)Proteasome Inhibitors in Mononuclear Blood Cells from Patients with Rheumatoid Arthritis. J Pharmacol Exp Ther. 2012 Apr;341(1):174-82. Epub 2012 Jan 10. (3/13/12 KBj ...
(A) IFN-γ production by cultured PBMCs was significantly reduced by CT treatment. PBMCs (106 cells/ml) were cultured for 72 h in the presence or absence of
A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor ...
Eady, JJ, Wortley, GM, Wormstone, YM, Hughes, JC, Astley, SB, Foxall, RJ, Doleman, JF and Elliott, RM (2005) Variation in gene expression profiles of peripheral blood mononuclear cells from healthy volunteers ...
BioAssay record AID 81792 submitted by ChEMBL: Anti-HIV-1 activity of phytohemagglutininin activated peripheral blood mononuclear cells infected with the macrophage-tropic HIV-1 virus (JRCSF) at 100 nM.
ATCC offers mononuclear cells from peripheral blood (PBMCs) and bone marrow. Mononuclear cells are a heterogeneous population of cells, consisting of lymphocytes (T cells, B cells, NK cells), monocytes, and dendritic cells.
Chemokine receptors (CKRs), the primordial receptors for primate lentiviruses, are sufficient to mediate virus-cell fusion. Several different fusogenic CKRs and related receptors provide a broad potential host cell range, presumably advantageous for viral spread within a given infected individual, and across species. By contrast, the additional constraint of obligatory CD4 binding, just prior to CKR engagement, radically restricts potential host cells within an individual (or lymph node microenvironment), and might also limit xenotransmission, as CD4 sequences vary among primates. In spite of these potential drawbacks, CD4 dependent entry for SIV and HIV is the rule rather than the exception, and is generally thought to have evolved by selection for 1) stabilization of virus-cell surface interactions, and 2) conformational shielding of readily neutralized CKR binding epitopes. CD4 binding residues of SIV and HIV envelope are recessed, (relatively hidden from immune detection) and may exhibit a strong
Peripheral blood mononuclear cells (PBMCs) can respond to dietary stimuli modulating the up-regulation of pro-inflammatory cell signaling, which is associated with metabolic disease and has been seen to be elevated in African American (AA) when compared to Caucasian American (CA) women. Little is known about the response of PBMCs to a high fat meal among women and the potential impact of ethnicity and/or weight status on this response. The purpose of this study was to examine PBMC response to consuming a high fat meal and the response to culturing PBMCs in media supplemented with lipid among AA and CA women, and to determine the impact that ethnicity and/or weight status may have on this response. Twenty-one women participated in the study: 10 AA (age: 28.00±6.60), 11 CA (age: 26.91±6.28), of whom 11 were lean (BMI
The availability of human peripheral blood mononuclear cells (PBMC) from healthy individuals and from patients with various diseases allows many studies on normal and abnormal functions of human immune cells. Because human and murine immune biology differs in many ways, it is important that various methodologies for studying human immunology are established. The two reports highlighted in this article demonstrate the usage of human PBMCs for mechanistic and pre-clinical human immune cell studies. Read more... ...
Everything you need for mononuclear cell culture: Cells isolated from umbilical cord blood or peripheral blood, and our optimized medium.
IL-1 gene expression was investigated in human blood mononuclear cells. IL-1 alpha and IL-1 beta mRNA were induced with LPS or TNF. Kinetic measurements on Northern blots revealed that these stimuli elicited qualitatively similar changes in IL-1 mRNA levels, and that expression of IL-1 mRNA was transient. IL-1 beta mRNA was the predominant mRNA species and remained elevated for somewhat longer than IL-1 alpha mRNA. TNF and IFN-gamma synergized to induce both species of IL-1 mRNA and IL-1 bioactivity. Transcriptional control, as measured by nuclear run on assays, partly determines the greater levels of IL-1 beta mRNA because the rate of IL-1 beta transcription was greater than that of IL-1 alpha. Cycloheximide (CHX) was able to induce IL-1 mRNA but did not induce transcription of either IL-1 gene. When added to cultures pretreated with TNF or LPS, CHX superinduced IL-1 mRNA, but IL-1 transcription was not increased. If added simultaneously CHX blocked TNF-induced IL-1 gene transcription, suggesting that
The research objective was to study frequency of antenatal infection and record probable clinical manifestations in 100 children with HCMV born from mother with HCMV in blood and mononuclear cells. The study identified poly-systemic internal organ damage in neonates due to prenatal HCMV. Research procedures involved study of 100 pairs of patients, Mother-Child tandem, using regular clinical assessment methods per algorithm and HCMV diagnostic methods: ELISA, affinity and avidity of HCMV antibodies, and HCMV genome identification via PCR method in blood plasma and mononuclear cells. Initial clinical disease manifested in 71% of children during late neonatal period. Children who died of HCMV (5%) were infected antenatal, and 39% were born prematurely. Embryonic stigma found in five cases. HCMVs affinity to different tissues during the process of embryogenesis leads of poly-systemic damage and results in various clinical manifestations in the postnatal period. HCMVs ability to invade mononuclear blood
Another method of separating blood is through apheresis. During this procedure, blood is drawn from a donor in the same way as a routine blood donation, however instead of being collected in a bag, the blood travels via tubing to a machine where the components are separated. The machine will separate the components using either the centrifuge process where the inside of the machine spins like a centrifuge or by filtration using a series of micro filters to separate components by size. After the desired components are collected the blood travels back through the tubing and is reinfused into the donor. Apheresis may be used on patients who exhibit severe symptoms of a disease and removing the constituent helps the symptoms. However this process must be done fairly frequently and is usually used as a last resort, after other methods to control the symptoms have failed ...
Interleukin-13 / IL13, 50 µg. Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine.
a cell that has 1 nucleus, used to refer to a subset of white blood cells (e.g., lymphocytes, monocytes). Mononuclear cells may circulate in the blood (peripheral blood mononuclear cells) or reside in the lymph nodes or other tissues ...
The evaluation of mitochondrial function remains crucial for the diagnosis of a spectrum of human pathologies. Peripheral blood provides an easily accessible source of primary human cells. However, mitochondrial respiratory studies in blood cells still require standardization. The procedures applied in bioenergetic assessments involve multiple steps in the pre-analytical, analytical and data analysis phase. The aim of this review is to summarize methods of preparing and applying peripheral blood mononuclear cells (PBMC) and platelets (PLT) for mitochondrial respiratory studies. For this purpose we report original data from several laboratories and literature data ...
Overview of the experimental design.Fractions of each sample, consisting of varying viral material spiked into human PBMCs were subjected to shotgun sequencing
UCSF scientists have engineered human immune cells that can precisely locate diseased cells anywhere in the body and execute a wide range of customizable responses, including the delivery of drugs or other therapeutic payloads directly to tumors or other unhealthy tissues.
Monocytes, recently classified as CD14++CD16- (classical), CD14++CD16+ (intermediate) and CD14dimCD16++ (non-classical) are considered to play a role in the pathogenesis of inflammatory bowel diseases (IBD). Anti-tumor necrosis factor (TNF)-α monoclonal antibodies (mAb) such as infliximab (IFX) dampen inflammation in diseases such as IBD and may in part exert their therapeutic effects via actions on cells that synthesize TNFα such as monocytes. Although clinically effective in many patients, anti-TNFα mAb do not work in some, and lose efficacy in others. A more detailed understanding of the actions of IFX on blood monocytes may provide important insights into the mechanism of action of anti-TNFα mAb and mechanisms of drug resistance. We therefore studied the acute effects of the anti-TNFα mAb IFX on blood monocytes and other mononuclear cells in a cohort of IBD patients. Multi-colour flow cytometry was used to analyse freshly isolated peripheral blood mononuclear cells (PBMCs) from Healthy ...
Wilson G.B.; Newell R.T.; Paddock G.V.; Burdash N.M.; Fudenberg H.H., 1979: Purification of 2 human thymus derived lymphocyte maturation factors from bovine lymph node and human mononuclear leukocyte extracts
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TY - JOUR. T1 - Response surface methodology to determine optimal cytokine responses in human peripheral blood mononuclear cells after smallpox vaccination. AU - Ryan, Jenna E.. AU - Dhiman, Neelam. AU - Ovsyannikova, Inna G.. AU - Vierkant, Robert A.. AU - Pankratz, V. Shane. AU - Poland, Gregory A.. PY - 2009/2/28. Y1 - 2009/2/28. N2 - Feasibility, amount of sample aliquots, processing time and cost are critical considerations for optimizing and conducting assays for large-population based studies. Well designed statistical approaches that quickly identify optimal conditions for a given assay could assist efficient completion of the laboratory assays for such studies. For example, assessment of the profile of secreted cytokines is important in understanding the immune response after vaccination. To characterize the cytokine immune response following smallpox vaccination, PBMC obtained from recently vaccinated subjects were stimulated with varying doses of live or UV-inactivated vaccinia virus ...
TY - JOUR. T1 - Transcriptional profiling of peripheral blood mononuclear cells in pancreatic cancer patients identifies novel genes with potential diagnostic utility. AU - Baine, Michael J.. AU - Chakraborty, Subhankar. AU - Smith, Lynette M. AU - Mallya, Kavita. AU - Sasson, Aaron R.. AU - Brand, Randall E.. AU - Batra, Surinder Kumar. PY - 2011/2/25. Y1 - 2011/2/25. N2 - Background: It is well known that many malignancies, including pancreatic cancer (PC), possess the ability to evade the immune system by indirectly downregulating the mononuclear cell machinery necessary to launch an effective immune response. This knowledge, in conjunction with the fact that the trancriptome of peripheral blood mononuclear cells has been shown to be altered in the context of many diseases, including renal cell carcinoma, lead us to study if any such alteration in gene expression exists in PC as it may have diagnostic utility. Methods and Findings: PBMC samples from 26 PC patients and 33 matched healthy ...
The aim of the first part of the thesis was to develop and validate an in vitro adherence assay involving porcine mononuclear cells (MCs) and porcine endothelium, present within gut and lymph node. Factors involved in MC / endothelium interactions were determined. In summary we found that cell adhesion in our assay system was temperature, Ca2+ and Mn2+ sensitive, required metabolic activity, was inhibited by the phosphorylated monosaccharide galactose 6-phosphate, and unaffected by the presence of mucus. These findings reflected certain aspects of in vivo cell adhesion, present within the in vitro assay used. The adhesion characteristics of porcine Peyer s patch (PP), peripheral blood (PB), and lymph node (LN) MCs to porcine gut and lymph node endothelium was examined and used as an guiding model for the future study of human MCs adherence. It was found that PP MCs adhered significantly better to gut endothelium than to LN endothelium and similarly LN MCs adhered significantly better to LN ...
Structural derivatives of 4-MTA, an illegal amphetamine analogue have been previously shown to have anticancer effects in vitro. In this study we report the synthesis of a series of novel 1,3-bis(aryl)-2-nitro-1-propene derivatives related in structure to 4-MTA. A number of these compounds containing a classic nitrostyrene structure are shown to have antiproliferative activities in vitro in a range of malignant cell lines, particularly against Burkitt?s lymphoma derived cell lines, whilst having no effect on `normal? peripheral blood mononuclear cells. Such effects appear to be independent of the serotonin transporter, a high affinity target for amphetamines and independent of protein tyrosine phosphatases and tubulin dynamics both of which have been previously associated with nitrostyrene-induced cell death. We demonstrate that a number of these compounds induce caspase activation, PARP cleavage, chromatin condensation and membrane blebbing in a Burkitt?s lymphoma-derived cell line, consistent ...
Studies in chronic untreated HIV-1 infection may shed more light on correlates of immune protection that may be utilized to develop effective preventive or therapeutic vaccines or drugs. The innate immune system, as the first to encounter the HIV virus upon exposure and infection may be critical in directing immune responses that can prevent, attenuate or cure infection. In this thesis I aimed to study the role of the innate cellular immunity in the immunopathogenesis of HIV-1 subtype A and D infection in Uganda. In Paper I, using whole blood from healthy blood bank donors, we established normal lymphocyte reference ranges for Ugandans and showed demographic differences that may influence immune responses to disease and vaccination. Additionally, utilizing cryopreserved peripheral mononuclear blood cells from chronic untreated HIV-1 infected persons we studied the phenotypes and function of natural killer cells, unconventional T cells and regulatory T cells plus their roles in HIV-1 infection ...
Systemic sclerosis is an autoimmune disease characterised by microvascular involvement and fibrosis affecting the skin, lung, and heart. To date, our understanding of the changes in MICs, T cells, and cytokines in SSc is still incomplete. In our study, we hypothesised that SSc is associated with alterations in MICs, T cell subsets (CD4/CD8), and cytokine production in the lung, blood, and skin. We also hypothesised that these alterations contribute to the genesis of the pulmonary and skin lesions and would therefore correlate with the clinicopathological characteristics of these lesions. We carried out this investigation to fill this existing gap in the literature and to test our hypotheses. Therefore, we examined 19 patients with SSc and six matched healthy individuals. Our results clearly showed the following: (1) SSc has a predilection for middle aged women; (2) MICs and cytokine concentrations (IL-1β and TNFα) are increased in the BALF, blood, and skin lesions of patients with SSc, (3) ...
Two parallel studies evaluated safety and immunogenicity of a prophylactic HIV-1 vaccine in 192 HIV-seronegative, low-risk volunteers. Modified vaccinia virus Ankara (MVA) and plasmid DNA (pTHr) expressed HIV-1 clade A gag p24 and p17 fused to a string of 25 overlapping CD8+ T cell epitopes (HIVA). Methods: These studies compared intramuscular, subcutaneous, and intradermal MVA at dosage levels ranging from 5×106-2.5×108 pfu. In Study IAVI-010, DNA vaccine was given as a prime at months 0 and 1, followed by MVA as a boost at months 5 and 8. In Study IAVI-011, MVA alone was given at months 0 and 2. Regular safety monitoring was performed. Immunogenicity was measured by the interferon (IFN)- ELISPOT assay on peripheral blood mononuclear cells (PBMC). Results: No serious adverse events were attributed to either vaccine; most adverse events were mild or moderate, although MVA resulted in some severe local reactions. Five vaccine recipients had at least one positive IFN- ELISPOT response, but none ...
Participants in this study were selected from a population of 10,049 women who participated in a population-based natural history cohort study of HPV and cervical neoplasia in the province of Guanacaste, Costa Rica. Details on the design and methods of the main cohort and the nested case-control study evaluating lymphoproliferative responses among HPV-positive and HPV-negative older women have been published (4, 8, 9). Briefly, we previously conducted a nested case-control study to examine lymphoproliferative responses within the Costa Rican natural history cohort where a group of women (n = 284) older than 45 who were infected with HPV were compared with a similarly sized control group of HPV-negative women (n = 291) of the same age distribution. Testing was done on peripheral blood mononuclear cells (PBMC) collected at the final visit of natural history cohort study (7-9 years after enrollment).. For the present evaluation, we were interested in screening for possible immune biomarkers ...
OBJECTIVE: The nonsynonymous polymorphism rs763361 of the CD226 gene, which encodes DNAX accessory molecule 1, which is involved in T cell costimulation pathways, has recently been identified as a genetic risk factor for autoimmunity. The purpose of this study was to test for association of the CD226 rs763361 polymorphism with systemic sclerosis (SSc) in European Caucasian populations. METHODS: CD226 rs763361 was genotyped in 3,632 individuals, consisting of a discovery sample (991 SSc patients and 1,008 controls) and a replication sample (999 SSc patients and 634 controls). All study subjects were of European Caucasian origin. Expression of CD226 was assessed on peripheral blood mononuclear cells obtained from 21 healthy donors genotyped for CD226 rs763361. RESULTS: The CD226 rs763361 T allele was found to be associated with SSc in both the discovery and the replication samples, showing the following results in the combined populations: odds ratio (OR) 1.22 (95% confidence interval [95% CI] ...
The active hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is an important modulator of the immune system, inhibiting cellular proliferation and regulating transcription of immune response genes. In order to characterize the genetic basis of variation in the immunomodulatory effects of 1,25D, we mapped quantitative traits of 1,25D response at both the cellular and the transcriptional level. We carried out a genome-wide association scan of percent inhibition of cell proliferation (Imax) induced by 1,25D treatment of peripheral blood mononuclear cells from 88 healthy African-American individuals. Two genome-wide significant variants were identified: rs1893662 in a gene desert on chromosome 18 (p = 2.32 x 10−8) and rs6451692 on chromosome 5 (p = 2.55 x 10−8), which may influence the anti-proliferative activity of 1,25D by regulating the expression of nearby genes such as the chemokine gene, CCL28, and the translation initiation gene, PAIP1. We also identified 8 expression ...
Yakults website for healthcare professionals, scientists and researchers. Information on probiotics, the science behind them, areas of potential health benefit and Yakult product information. There is also a resource centre which includes current and past issues of our quarterly newsletter, publications, key references and conference dates. Information on this website has been put together by Yakults science team which consists of a microbiologist, dietitians and nutritionists.
Peripheral blood mononuclear cells (PBMC) serve a sentinel role allowing the host to efficiently sense and adapt to the presence of danger signals. Herein we have directly compared the genome-level expression patterns (microarray) of a human PBMC model (THP-1 cells) subjected to one of two canonical danger signals, heat shock or lipopolysaccharide (LPS). Based on sequential expression and statistical filters, and in comparison to control cells, we found that 3,988 genes were differentially regulated in THP-1 cells subjected to LPS stress, and 2,921 genes were differentially regulated in THP-1 cells subjected to heat shock stress. Venn analyses demonstrated that the majority of differentially regulated genes (≥ 70%) were uniquely expressed in response to one of the two danger signals. Functional analyses demonstrated that the two danger signals induced expression or repression of genes corresponding to unique pathways, molecular functions, biological processes, and gene networks. In contrast, there
Lymphocyte Separation Medium is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes.
Description of Subjects: Fenway Health medical patients who have had at least 2 HIV primary care visits at Fenway in a 12 month period and are 18 years or older ...
In vitro experiments demonstrated that B-I09 significantly reduces human DC migration (34.4% v 12.8% CCL19 chemotaxis, P , .05) and stimulatory potency against allogeneic T cells (90.5% v 30.7% 5-day proliferation, P , .01) compared to a DMSO control. To test the efficacy of B-I09 in vivo, NSG mice were transplanted with a human skin graft (1 cm2) and later injected with 5x106 human peripheral blood mononuclear cells (PBMC) allogeneic to the skin. B-I09 was administered at 30 mg/kg by intraperitoneal injection five days a week for three weeks. Blinded pathologic scoring of recipient skin grafts at day +21 showed B-I09 significantly decreased tissue destruction by the allogeneic T cells (P , .01). A reduction in xenogeneic GVHD (murine liver, P , .05) was also observed. B-I09 also decreased tissue destruction in liver and lung grafts by decreasing lethal-grade GVHD (stage 2) to non-lethal-grade GVHD (stage 1 in lung and stage 0.5 in liver).. ...
Mouse monoclonal antibody raised against native CD14. Native purified from human peripheral blood mononuclear cells. (MAB6045) - Products - Abnova
Mouse monoclonal antibody raised against native B3GAT1. Native purified from human peripheral blood mononuclear cells. (MAB6072) - Products - Abnova
pluriSelect separation tubes have been developed for optimal separation of leukocytes and peripheral blood mononuclear cells (PBMC) from whole blood and bone marrow. pluriMate and TwinSpin prevents you from time-consuming and laborious overlaying of the sample material. During centrifugation, Leukocytes, lymphocytes and PBMCs are separated from unwanted erythrocytes and granulocytes, depending on the density gradient media ...