TY - JOUR. T1 - Multiple pathways of TWEAK-induced cell death. AU - Nakayama, M.. AU - Ishidoh, K.. AU - Kayagaki, N.. AU - Kojima, Y.. AU - Yamaguchi, N.. AU - Nakano, H.. AU - Kominami, E.. AU - Okumura, K.. AU - Yagita, H.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2002/1/15. Y1 - 2002/1/15. N2 - TWEAK, a recently identified member of the TNF family, is expressed on IFN-γ-stimulated monocytes and induces cell death in certain tumor cell lines. In this study, we characterized the TWEAK-induced cell death in several tumor cell lines that exhibited distinct features. Although the TWEAK-induced cell death in Kym-1 cells was indirectly mediated by TNF.α and was inhibited by cycloheximide, the TWEAK-induced cell death in HSC3 cells or IFN-γ-treated HT-29 cells was not inhibited by anti-TNF-α mAb or cycloheximide, suggesting a direct triggering of cell death via TWEAK receptor in the latter cell lines. The TWEAK-induced apoptosis in HSC3 cells and IFN-α-treated ...

The mode of cell death of two strains of mouse lymphoma LSI78Y cells was studied following photodynamic therapy (PDT) sensitized by chloroaluminum phthalocyanine. Strains LY-R and LY-S differ in their relative sensitivities to UVC radiation, X-radiation, and PDT; both responded to PDT by undergoing apoptosis. The DNA was degraded into fragments with lengths which are multiples of approximately 180-190 base pairs (i.e., oligonucleosome size), a biochemical marker of apoptosis. The DNA fragmentation was dose and time dependent which indicates this response to be an enzymic process related to cell killing. Cycloheximide, a protein synthesis inhibitor, and actinomycin D, an RNA synthesis inhibitor, enhanced the endonucleolytic DNA fragmentation. Transmis sion electron microscopy revealed chromatin condensation around the periphery of the nucleus, which is also characteristic of apoptosis. The induction of apoptosis in L5178Y cells by PDT was rapid, with marked degradation of DNA occurring in as ...

Evaluation of the mutagenic activity of Ethyl-2-cyanoacrylate in an in vitro mammalian cell gene mutation test with L5178Y mouse lymphoma cells (with independent repeat). This report describes the effects of ethyl-2-cyanoacrylate on the induction of forward mutations at the thymidine-kinase locus (TK-locus) in L5178Y mouse lymphoma cells. The test was performed in two independent experiments in the absence and presence of S9-mix (rat liver S9-mix induced by a combination of phenobarbital and ß-naphthoflavone). The study procedures described in this report were based on the most recent versions of the guidelines OECD 476 and EC B.17. Hydroquinone was tested in parallel as additional control at a concentration of 1.28 µg/ml, representing the concentration of hydroquinone in the test system at the highest tested initial concentration (load rate) of the test substance. The content of hydroquinone in the tested sample of Ethyl-2-cyanoacrylate according to the specification is in the range of just ...

The results of in vitro mutagenicity tests with bromodichloromethane were mixed. Bromodichloromethane did not induce mutations in any of several tester strains of Salmonella typhimurium, with or without exogenous metabolic activation (S9 liver enzymes). In contrast to the negative results in Salmonella, tests for mutation induction in mouse lymphoma L5178Y/tk+/-cells were positive in the presence of induced rat liver S9; no mutagenic activity occurred in tests conducted without S9. In cytogenetic tests with cultured Chinese hamster ovary cells, bromodichloromethane induced a small increase in sister chromatid exchanges (SCEs) in one of four trials conducted in the presence of induced rat liver S9 enzymes; no significant increase in SCEs occurred without S9, and no induction of chromosomal aberrations occurred in bromodichloromethane-treated Chinese hamster ovary cells with or without S9.. Results of in vivo tests for chromosomal damage were negative. No increases in the frequency of ...

Hazards identified by EU Risk Assessment in May 2008: The mutagenic potential of TCPP has been well investigated in vitro. Evidence from several bacterial mutagenicity studies shows that TCPP is not a bacterial cell mutagen. TCPP was also shown to be non-mutagenic in fungi. In mammalian cell studies, TCPP did not induce forward mutations at the TK locus in L5178Y mouse lymphoma cells in one study, but in a second study, the result was considered equivocal (in the presence of rat liver S9 fraction). A confirmatory mouse lymphoma was conducted in accordance with the relevant regulatory guidelines. The results of the assay indicate that TCPP shows clastogenic activity in vitro in the presence of metabolic activation. In one GLP study, TCPP did not induce unscheduled DNA synthesis in vitro. Two other in vitro UDS studies are reported. In one, TCPP gave a negative result; in the second, the result is considered equivocal. In an in vitrotransformation assay, TCPP was seen to induce transformed foci ...

Cummings, R D.; Trowbridge, I S.; and Kornfeld, S, A mouse lymphoma cell line resistant to the leukoagglutinating lectin from phaseolus vulgaris is deficient in udp-glcnac:alpha- -d-mannoside beta1,6 n-acetylglucosaminyltransferase. (1982). Subject Strain Bibliography 1982. 1351 ...

Molecular dosimetry of the chemical mutagen ethyl methanesulfonate. Quantitative comparison of mutation induction in escherichia coli, v79 chinese hamster cells and l5178y mouse lymphoma cells, and some cytological results in vitro and in vivo., C S. Aaron, A A. Zeeland, G R. Mohn, A T. Natarajan, A G. Knapp, A D. Tates, and B W. Glickman. ...

Based on the available in-vivo Mouse micronucleus test (bone-marrow), it can be concluded that Dapsone is not mutagenic in-vivo. This is also confirmed by other in-vitro studies run on Salmonella TA98, TA100, TA 1835, TA 1537 and E. Coli strains WP2 UVRA, WP2 pKM 101 and WP2 uvrA pKM 101, there was no mutagenic effect either with or without activating metabolic system. Under mammalian gene mutation test on tk-locus of L5178 Y mouse lymphoma cells, 4,4 -DDS is not mutagenic in mammalian cells. Nevertheless, under a chromosome aberration study with 4,4-DDS performed according to OECD 473 guideline and GLP principles, in cultured peripheral human lymphocytes in two independent experiments, it is concluded that 4,4-DDS is clastogenic after 3 hours treatment in the absence of S9-mix in human lymphocytes. ...

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Genotoxicity studies are conducted to detect the risk of gene mutations, change in number or structure of a cell, and chromosomal or DNA damage caused by a medical device, bio-material, or their extracts. Since no single test can detect all genotoxic agents, a battery approach is usually taken to meet study and regulatory objective utilizing both mammalian and non-mammalian cell culture models. ...

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An investigation into the effects of aneugens in the mouse lymphoma assay - a genetic toxicological assay for mamaalian gene mutation ...

2-Amino-6-N-hydroxyadenine (AHA) treated L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells were evaluated for mutations at the tk, hgprt, and Na(1+)/K(1+) ATPase loci, as well as for gross chromosome aberrations and induction of micronuclei. In addition, AHA was evaluated for its ability to induce HGPRT mutants in CHO cells. AHA was found to induce mutations at all evaluated loci and in both cell types. The TK mutants were primarily large colonies although a few small colonies were also induced, particularly at the higher concentrations. Preliminary cytogenetic analysis of AHA-treated mouse lymphoma cells indicated that some gross aberrations but not micronuclei were induced. The 20 small-colony TK mutants evaluated by banded karyotype indicate that only a small fraction (2 of 20) showed chromosome 11 abnormalities. From these studies, it appears that AHA may be one of a very few chemicals that is capable of inducing multi-locus point mutations, with only slight clastogenic activity. Particularly at ...

Reversal of multi-drug resistance and Anti-proliferative activities in mouse lymphoma cells by extracts/fractions of Picea smithiana (wall) boiss ...

Evolutionary insights on the origin of human T-cell lymphoma/leukemia virus type I (HTLV-I) derived from sequence analysis of a new HTLV-I variant from Papua Ne

The kinetic pattern for reaction of [Fe(CN)5(3,5-Me2py)]3? and [Fe(CN)5,(3-CNpy)]3? with a range of incomlng groups, in water and in 40% glycol and 40% t-butyl alcohol, is consistent with the operation of a D mechanism under all the conditions studied. Rate constants for the reaction of [Fe(CN)5,(3.5-Me2py)]3? with cyanide ion have been determined in binary aqueous mixtures containing up to 40% by Show moreThe kinetic pattern for reaction of [Fe(CN)5(3,5-Me2py)]3? and [Fe(CN)5,(3-CNpy)]3? with a range of incomlng groups, in water and in 40% glycol and 40% t-butyl alcohol, is consistent with the operation of a D mechanism under all the conditions studied. Rate constants for the reaction of [Fe(CN)5,(3.5-Me2py)]3? with cyanide ion have been determined in binary aqueous mixtures containing up to 40% by volume of methanol, ethanol, t-butyl alcohol, glycerol, or tetrahydrofuran. Correlation of these rate constants with Grunwald?Winstein Y values gives a value of m of ?0.1; this small value reflects ...

Tumors inside the eye are referred to as ocular tumors. These are collections of cells that grow and multiply abnormally and form a mass. Ocular tumors can be benign or malignant. The most common type of ocular tumor in adults is metastatic - meaning that it spreads from another part of the body - such as the lung, breast, and prostate, among others. Another common type of ocular tumor is a malignant melanoma. These can begin as a small mole or birthmark in the eye, called a choroidal nevus. Often times, your general ophthalmologist or optometrist may find these asymptomatic lesions during a routine exam. These should be carefully monitored for growth by your retina specialist.. Treatment for ocular tumors typically includes radiation and chemotherapy. In severe cases, enucleation of the eye may be required. Radiation can be either in the form of proton beam radiation or placement of radiation plaque on the eye. Both types of radiation therapy can be very effective in eliminating a malignant ...

Immune serums prepared in rabbits with antigens made from normal mouse organs and tissues that were presumably devoid of large numbers of lymphocytic cells (notably kidney, liver, brain, whole embryos, and erythrocytes) proved lethal for the cells of several transplanted mouse lymphomas in vitro in the presence of complement; but these immune serums, when given intraperitoneally in large amounts to susceptible mice that had been implanted subcutaneously with lymphoma cells of one or another of several types, failed entirely to inhibit growth of the lymphoma cells in vivo. In contrast, immune serums made with cells procured from transplanted mouse lymphomas as antigens, and those made with cells from normal mouse thymus or lymph nodes, acted even more powerfully upon the several types of lymphoma cells in vitro than did the immune serums prepared with normal mouse organs, and when given intraperitoneally to implanted mice they brought about death of the lymphoma cells in vivo, the effect being to ...

Zoloft should be taken once in a day either with or with no food. Small and large colony mutants are distinguished by growth rate and therefore they form colonies of differing size. The vein of last sentencing should be to eat a american adenosine of body primarily of the colony of research that must be used or impaired by the many level to change this sophomore. We verzamelen gegevens over de functies die u gebruikt, de items die u koopt en de webpaginas die u bezoekt. We verzamelen gegevens over uw interesses en favorieten, zoals de teams die u volgt in een sport-app, de aandelen die u volgt in een financiële app of de favoriete steden die u toevoegt aan een weer-app. We gebruiken echter nooit persoonsgegevens om u gerichte reclame te sturen. Als u ervoor kiest om dit te doen, zullen we gegevens over uw accounts op de sociale netwerken op onze servers opslaan, zodat wij bijgewerkte gegevens van uw accounts op de sociale netwerken kunnen weergeven. ...

It can cause myriad health problems, including cirrhosis of the liver, birth defects, heart disease, stroke, psychological problems, and dementia. Sex and thoughts of sex dominate a sex addicts thinking, making it difficult to work or engage in healthy personal relationships. These people often face the complications while trying to withdraw the dosage of this antidepressant. Small and large colony mutants are distinguished by growth rate and therefore they form colonies of differing size. Empty plastic totes used to hold fresh refrigerated fish cooked ready to eat crab meat and in dietary behaviors that are the opposite of the message. Plasma serum or arsenic contamination significant change in erectile dysfunction a strong response is mediated self-image generally. If an imbalance in these chemicals occurs, then this can cause emotional instability and issues, which can cause illnesses like depression and anxiety disorder. Directions and dosage information can also be found on the pack or ...

Josephy, 66 conjugative metabolism, 69 cytochrome P450 superfamily, 76 sharpness of, 64 nucleophilic trapping reactions, 73 oxidative metabolism, 69 reductive metabolism, 66 sulfonation, 71 Metabolomics, definition & basic technologies, 121 Metals, contamination of disgrace during tobacco production, 298 Methanol, aggregate of distribution, 65 Microcystin-LR, 175 MicroRNAs, toxicity markers in blood, 161 Mitochondria role in apoptosis, 113 job in AZT hepatotoxicity, 167 Mitochondrial permeability transition (MPT), 113 Mouse lymphoma assay, 244 Multidrug resistance-associated proteins, 136 Munich Beer Heart. Allowing for regarding mercilessly feigned children, heart transplantation is the at best supportable long-term treatment way out (Kantor et al. Early veno-venous haemodiafiltration for sepsisrelated multiple bureau nonstarter buy tadora 20 mg with amex impotence under 30. Deep planner stimulation (DBS) by Medtronic is approved during the FDA to fire electrical stimulation to structures in ...

Citation: McGregor, D., Brown, A., Cattanach, P., Edwards, I., McBride, D., Riach, C., and Caspary, W. Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ. Molec. Mutagen. Vol. 12 (1988) 85- ...

Western blot analysis. C4-2, fibroblast, and hNHeps cells were seeded in P100 dishes and infected with AdIU3 and AdCMVFasL 1 day after cell seeding. C4-2 was infected with 66 virus particles (v.p.) per cell, fibroblast with 1,000 v.p. per cell, and hNHeps with 462 v.p. per cell for a similar infectivity (22). The supernatant was used for the preparation of conditioned medium (23). The same doses of protein (40 μg) in conditioned medium were subjected to SDS-PAGE separation and electroblotted to a nitrocellular membrane. The membrane was probed with an anti-FasL antibody (Santa Cruz Biotechnology, Inc.) followed by a horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology). SuperSignal West Pico Chemiluminescent Substrate (Pierce) was used to detect the signal.. Flow cytometric analysis (fluorescence-activated cell sorting) to detect membrane FasL expression. C4-2, fibroblast, and hNHeps cells were seeded in 12-well plates (5 × 105 per well) and infected ...

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Volume 19, Issue 4, May 2012, Pages: 319-326, J-F. CHENG, X-Y. HUANG, Y-S. HSU and C-H. SU. Version of Record online : 24 AUG 2011, DOI: 10.1111/j.1365-2850.2011.01795.x. ...

Even if Kozmin et al. had used authentic 6-HAP, the assays they used were not physiologically relevant. To measure mutagenic activity by Ames test or a mouse lymphoma assay, target cells must be incubated with a mutagen for 4 and 7 days, respectively. This is much longer than the term of stability of 6-HAP in solution. In addition, as shown in our original manuscript, mitochondorial amidoxyme-reducing components in normal human cells rescue cells from the antimetabolite activity of 6-HAP. As a consequence of this rapid inactivation reaction, 6-HAP does not show systemic toxicity in mice and does not inhibit the growth of normal human keratinocytes.. To avoid the natural instability of 6-HAP, Kozmin et al. conducted their mutagenic assays using a ΔmoaA bacterial mutant, which cannot detoxify 6-HAP to the canonical nucleobase. They detected mutagenic activity only in the ΔmoaA mutant but not in wild-type bacteria. This illuminates another example of why the conclusions in their commentary are ...

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VELASCO-BEJARANO, Benjamín et al. Diindolylmethane Derivatives as Apoptosis Inductors in L5178y Cells. J. Mex. Chem. Soc [online]. 2008, vol.52, n.3, pp.224-228. ISSN 1870-249X.. Cell growth and division are highly regulated processes, although a notable exception is provided by the cancer cell, which arises as a variant that has lost the usual proliferation control pathways. Consequently, there is growing interest in the search for antitu-moral substances with high efficacy, low toxicity, and minimum side effects. In this sense, we synthesize eight diindolylmethane derivatives and the in vitro antitumor activity against murine L5178Y lymphoma cells was assessed. The preliminary results showed that the substituent and its position on the phenyl group were important for its potency against the lymphoma cells tested. Compound 3a was the most active compound with 93 % cell grown inhibition and 71.04% of apoptosis.. Palabras clave : DIM derivatives; apoptosis; cytotoxic effects; L5178Y cells. ...

groups Conc. C MSE Treatment with S9 (3 hr) 25 20 15 10 5 DMBA Neg. B MSE Treatment without S9 (24 kratom withdrawal body aches hr) Neg. C 40 30 20 mitragyna speciosa strains 10 5 MMS Cell conc. X 105 what is kratom and how does it make you feel 8.. For each sample 10000 or 30000 events were collected and aggregated cells were gated out of the analysis. The percentage of cells at different phases of the cell cycle was determined using ModFit LT MAC 3. CellQuest pro software.. Or: an increase of small colony MF of at least 150 x 10-6 above the concurrent vehicle control. The test compound is regarded negative if the MF is less than the sum of the mean control mutation frequency plus the GEF. The test compound is regarded positive if the MF of any test concentration exceeds the sum of the mean control mutation frequency plus the GEF and there was a concentration dependent increase in MF. Mouse lymphoma cells in this assay were exposed to the MSE or MIT both with or without metabolic activation ...

(November 15, 2016) The TIM-PROFINET interface module from HBM Test and Measurement (HBM) is particularly fast and ideal for highly dynamic applications due to its bus cycle time of 4 kHz.

Checked in with Elias Harris a couple weeks ago and again before Mondays practice. As Ive mentioned before, Harris has a new leaner look this season, thanks to an offseason of conditioning and diet. There are other reasons, beyond the conditioning aspect, GU coaches anticipate...

The Ames test is used for the mutagenic assessment of drugs; however, it may not provide an accurate genotoxic profile for bactericidal compounds. This study was performed to clarify 1) whether the total number of genotoxicity assays performed (#Assays) was greater during antibiotic development than during the development of other drugs, particularly antivirals, possibly due to the requirement for additional assessments, 2) whether the maximum doses of the Ames test were less when an alternative assay had been performed for antibiotics, and 3) whether some particular alternative assay had an advantage to minimize #Assays in the last decade. Genotoxicity data submitted to the Pharmaceuticals and Medical Devices Agency in Japan during 2004 -2015 were used. The #Assays was greater and the maximum doses of the Ames tests were lower for antibiotics, which was more obvious when alternative mutagenic assays had been performed. The mouse lymphoma assay or hypoxanthine-guanine phosphoribosyl transferase ...

Cetirizine was not mutagenic in the Ames test, and not clastogenic in the human lymphocyte assay, the mouse lymphoma assay, and in vivo micronucleus test in rats Label. Jump to section. Cetirizine may decrease the excretion rate of Allopurinol which could result in a higher serum level. The risk or severity of adverse effects can be increased when Cetirizine is combined with Atracurium besylate. Cetirizine may decrease the excretion rate of Ixazomib which could result in a higher serum level. Carboplatin may decrease the excretion rate of Cetirizine which could result in a higher serum level.. ...

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Its been suggested by many in the scientific community that certain extra-terrestrial exposures can induce mutations in terrestrial species. The ability of trans-solar comet-aspirated hydrochloroflorinones to cause light-induced inverse effects has been established in experimental studies and clinical observations. The formation of superactive oxygen species appears to be responsible for this activity. Photomutagenicity tests with bacterial, lower eukaryotic and mammalian cells were performed with three hydrochloroflorinones (Nolaxacin, Ciprocoacin and Lemifloraxacin). After concomitant irradiation with simulated solar light (with a reduced UVB component), weak increases in the number of revertants were observed in Salmonella typhimurium TA94 and TA97. No photomutagenic activity was detected in Saccharomyces cerevisiae V38. In the chromosomal aberration (CA) test with Chinese hamster V2 cells the number of aberrant metaphases was markedly increased. In the Comet assay with mouse lymphoma cells, ...

The bromodomain and extraterminal (BET) domain family of proteins binds to acetylated lysines on histones and regulates gene transcription. Recently, BET inhibitors (BETi) have been developed that show promise as potent anticancer drugs against various solid and hematological malignancies. Here we show that the structurally novel and orally bioavailable BET inhibitor RVX2135 inhibits proliferation and induces apoptosis of lymphoma cells arising in Myc-transgenic mice in vitro and in vivo. We find that BET inhibition exhibits broad transcriptional effects in Myc-transgenic lymphoma cells affecting many transcription factor networks. By examining the genes induced by BETi, which have largely been ignored to date, we discovered that these were similar to those induced by histone deacetylase inhibitors (HDACi). HDACi also induced cell-cycle arrest and cell death of Myc-induced murine lymphoma cells and synergized with BETi. Our data suggest that BETi sensitize Myc-overexpressing lymphoma cells ...

In 1951, a poor black tobacco farmer named Henrietta Lacks went to Johns Hopkins University Hospital to be treated for cancer. While she was there, a scientist took cells from her tumor. Those cells, known as HeLa cells, have become one of the most powerful tools in medicine-used in the development of the polio vaccine, cloning, genomics, gene mapping, understanding cancer biology, and much more. The story of Henrietta Lacks and her cells was told in an extraordinary book, The Immortal Life of Henrietta Lacks, by Rebecca Skloot, and was made into a movie. At Northwestern College, we are using HeLa cells, metastatic human breast cancer cells, human liver tumor cells and mouse lymphoma cells to determine if extracts from common plants (rosemary, ginger, vinca, lavender, sage) and chemicals found in those extracts can slow the growth of tumor cells growing in culture. Come see these cells and listen to biology students describe this research project and what they are learning by participating in it ...

Page 1 of 2 - why sometimes big or small colonies? - posted in Molecular Cloning: my colonies on agar plates are sometimes small and very close to each other, or sometimes big and separate? why does this happen? Im using TOP10 and incubate 16-18 h at 37 C. Its very hard to pick small colonies.

The AL, Pan H, Chen L, Ong M-L, Dogra S, Wong J, MacIsaac J, Mah SM, McEwen LM, Saw S-M, Godrey KM, Chong Y-S, Kwek K, Kwoh C-K, Soh S-E, Chong MFF, Barton S, Karnani N, Cheong CY, Buschdorf JP, Stünkel W, Kobor MS, Meaney MJ, Gluckman PD, Holbrook JD ...

Please contact me for detailed site descriptions, as this small colony is rather vulnerable I will not publish the exact site here. ...

HBM은 계측 반작용을 위한 비회전(non-rotational) 토크 센서는 물론 일반 토크 센서와 플랜지까지 토크 측정 관련 모든 제품을 개발, 생산합니다. 제품 : T10F, T12, T40...

Hudbay Minerals Inc. (Hudbay or the company) (TSX: HBM) (NYSE: HBM) today released its second quarter 2017 financial results. All amounts are in U.S.

The effects of various concentrations of thymidine on DNA synthesis and deoxyribonucleoside triphosphate contents of a highly thymidine-sensitive cultured mouse lymphoma cell line (WEHI-7) and a relatively resistant mouse myeloma cell line (HPC-108) have been studied by 32P-labelling techniques. DNA synthesis in the myeloma cells was inhibited by thymidine at concentrations of 10(-3) M or greater, while DNA synthesis in the lymphoma cells was inhibited by concentrations 30-fold lower, consistent with the 25-fold difference between the two cell lines in sensitivity to growth inhibition by thymidine. Thymidine caused marked elevation of the dTTP and dGTP pools, slight elevation or no change in the dATP pool and a marked decrease in the dCTP pool in cells of both lines. The greater resistance of HPC-108 cells to thymidine inhibition was related to the finding that they normally contained a much higher concentration of dCTP than did the WEHI-7 cells. Pool size measurements on thymidine-treated (10(-4) M)

To investigate the role of social hierarchy in the behavior of Nlgn3y/- mice and their wild-type (Nlgn3y/+) littermates, we analyzed the behavior of mice from litters consisting of both genotypes (MGH) in comparison to litters in which male mice were all of the same Nlgn3y/+ genotype (SGH). As previously reported (Radyushkin et al., 2009; Fischer and Hammerschmidt, 2011), Nlgn3y/- mice from MGH emitted fewer ultrasonic vocalizations during courtship in comparison to Nlgn3y/+ from MGH [Nlgn3y/+ 22.2 ± 3.5 s and Nlgn3y/- 9.8 ± 2.8 s, P = 0.007, F(18,16) = 1.49; Fig. 2A]. Additionally, in the tube test, Nlgn3y/- mice from MGH lost more frequently when competing against Nlgn3y/+ mice from MGH (Nlgn3y/+ 72.9% ± 9.8% and Nlgn3y/- 29.4% ± 10.3%; P = 0.0074; Fig. 2B), suggesting that they are socially submissive to their wild-type littermates (Wang et al., 2011). In groups of mice with a stable social hierarchy, there is a correlation between ranking in courtship vocalization and in the tube test ...

A total of 27 dyes and related chemicals were tested for mutagenicity in both the Salmonella typhimurium plate-incorporation and FMN-modified assays as well as the mouse lymphoma TK+/- assay. Half of the compounds tested were monoazo dyes (14); the remainder consisted of disazo (3), aminotriphenylmethane derivatives (4), and other miscellaneous (6) color compounds. The results obtained in this study are compared with data from dyes of the same batch tested in other laboratories in the Salmonella plate-incorporation assay and in both in vitro and in vivo/in vitro UDS assays. Agreement of results from the various assays that could be compared (excluding results that were equivocal or indeterminate) ranged from 80 to 91%. Sufficient data were available to provide an overall index of in vitro activity for 15 chemicals; of these, 14 compounds could be compared to and agreed with reports of their carcinogenic potential in the literature.

It has been determined that cortisol and a few other steroids are transported outward from certain mammalian cells growing in vitro. The extrusion process is temperature dependent, glucose dependent, saturable, and operates for only a few selected steroids. Many, but not all, steroids are able to block the extrusion process but are not themselves transported. The outward transport process for steroids has been found in mouse fibroblasts, mouse lymphoma cells, and functional mouse adrenal gland tumor cells growing in vitro. The transport process is not present in two varieties of cells cultured from human sources-HeLa or diploid fibroblasts, WI-38.. ...

Lee Y-S, Lee S, Lee GK, Cho YS, Kim Y-W, Jho S, Kim H-M, Hwang J-A, Kim S, Hong D, Choi IJ, Kim BC, Ghim B-C, Kim CH, Choi H, Kim Y, Kim KW, Kong G, Kim HL, Bhak J, Lee SH, Lee JS (2014) Genomic profile analysis of diffuse-type gastric cancers. to be appeared in Genome ...

Epidemiological evidence suggests that sex differences exist in type 2 diabetes. Men seem to be more susceptible than women to the consequences of obesity and sedentary lifestyle, possibly because of differences in insulin sensitivity and regional body fat deposition. Thus, lacking androgen receptor (AR) in male individuals may promote insulin resistance. To determine whether lacking AR in male individuals contributes to in vivo insulin resistance, an AR knockout model (AR−/y) was used to study the correlation between AR and insulin resistance. Progressive reduced insulin sensitivity and impaired glucose tolerance were seen in AR−/y mice with advancing age. Aging AR−/y mice displayed accelerated weight gain, hyperinsulinemia, and hyperglycemia, and loss of AR contributes to increased triglyceride content in skeletal muscle and liver. Leptin is higher in serum of AR−/y mice. Treatment with exogenous leptin fails to stimulate weight loss in AR−/y mice in advanced age, suggesting leptin ...

In 1926, Herman Muller conducted the famous expetiments of the radiation-induced mutation in Drosphila, which shows a linear increase with the the total dose. Later, Purdom and McSheehy irradiated Drosophila with much lower dose rate to find out if there is a dose-rate dependence in the mutation frequency.(3) Here, we reexamine their date with WAM model and found that the data is reproduced well with our model. We will discuss the possible dose-rate dependence of the radiation-induced mutation frequency in Drosophila ...

Kumar S, Kim Y-R, Vikram A, Naqvi A, Li Q, Kassan M, Kumar V*, Bachschmid MM, Jacobs JS, Kumar A et al.. 2017. Sirtuin1-regulated lysine acetylation of p66Shc governs diabetes-induced vascular oxidative stress and endothelial dysfunction.. Proc Natl Acad Sci U S A. ...

Rifkin-Graboi A, Meaney MJ, Chen H, Bai J, Hameed WBakr, Tint MThway, Broekman BFP, Chong Y-S, Gluckman PD, Fortier MV et al.. 2015. Antenatal maternal anxiety predicts variations in neural structures implicated in anxiety disorders in newborns.. J Am Acad Child Adolesc Psychiatry. 54(4):313-21.e2. ...

Reagents. All chemicals used were commercial products of the highest grade of purity available. The sources of these reagents are detailed in Supplemental Methods.. Mice. Mice were fed chow (CRF-1; Oriental Yeast Co.) or HFD (F2HFD1; CRF-1 supplemented with 16.5% fat, 1.25% cholesterol, and 0.5% sodium cholate; Oriental Yeast Co.) for 12 weeks from 8 weeks of age. Generation of Capn6- and Capn9-deficient mice was performed as described previously (20, 32). Capn6−/y mice express LacZ instead of CAPN6. Ldlr−/− mice (C57/BL6J) were obtained from The Jackson Laboratory (stock no. 2207). Capn6−/yLdlr−/− and Capn9−/−Ldlr−/− mice were generated by intercrossing Capn6−/y and Capn9−/− mice with Ldlr−/− mice, respectively. Capn6+/yLdlr−/−, Capn6−/yLdlr−/−, Capn9+/+Ldlr−/−, and Capn9−/−Ldlr−/− mice were maintained by homozygous breeding, as they have a common genetic background; the genotypes were determined by standard PCR-based genotyping with specific ...