TY - JOUR. T1 - Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice. AU - Wu, T. C.. AU - Wan, Yu-Jui Yvonne. AU - Damjanov, I.. PY - 1983/6. Y1 - 1983/6. N2 - Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement ...
Humans dont have the proper enzymes necessary to digest lectins.. As they pass through the stomach, lectins remain largely unaltered. When they reach the intestines, they can attach to the lining of the gut (6).. In one study that was conducted on rodents, lectins made it through the stomach and attached themselves to the small intestines.. They temporarily thickened the walls of the small intestines, affecting their ability to absorb nutrients.. They also changed the length and function of the entire digestive tract. These effects were mostly reversed upon elimination of lectin from the diet (7).. Lectins can enhance pancreas growth and increase the release of digestive enzymes (8).. Lectins make it difficult for the body to maintain its own cells.. Therefore, people with damage from lectins may experience more digestive problems. This may occur gradually (9, 10).. Phytohaemagglutinin are the most commonly studied lectins. Kidney beans are one of the main sources of these proteins.. If you eat ...
Lectins from various sources have been shown to exhibit potent antiviral properties by inhibiting infection of clinically important viral pathogens. The antiviral activity of lectins is largely attributed to direct binding to viral envelope glycans and preventing entry of the virus into cells. Several lectins, particularly plant lectins with affinity toward mannose and N-acetylglucosamine (GlcNAc) sugar moieties, have been identified as potential therapeutic agents in the prevention of viral transmission in human immunodeficiency virus (HIV) and coronaviruses (SARS-CoV and MERS-CoV) [1].. Coronaviruses are enveloped single-stranded RNA viruses that contain at least four structural proteins: the membrane (M), envelope (E), spike (S) and nucleocapsid (N) protein. The heavily glycosylated S protein mediates virus-cell attachment and fusion. Mannose-binding lectins interfere with the coronavirus entry process by binding to the high-mannose type N-glycans of SARS-CoV via the S protein, preventing ...
RIBEIRO MELO-JUNIOR, Mario et al. Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry. Int. J. Morphol. [online]. 2008, vol.26, n.4, pp.967-972. ISSN 0717-9502. http://dx.doi.org/10.4067/S0717-95022008000400030.. This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4,J.m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A ...
Lectins, a group of proteins that bind to cell surface carbohydrates and play important roles in innate immunity, are widely used experimentally to distinguish cell types and to induce cell proliferation. Eel serum lectins have been useful as anti-H hemagglutinins and also in lectin histochemistry as fucose-binding lectins (fucolectins), but their structures have not been determined. Here we report the primary structures and the sites of synthesis of eel fucolectins. Eel serum fucolectins were separated by two-dimensional gel electrophoresis and sequenced. cDNA cloning, based on the amino acid sequence information, and Northern blot analysis indicated that 1) the fucose-binding lectins are secretory proteins and have unique structures among the lectins, exhibiting only weak similarities to frog pentraxin, horseshoe crab tachylectin-4, and fly fw protein; 2) there are at least seven closely related members; and 3) their messages are abundantly expressed in the liver and in significant levels in ...
This title introduces groundbreaking approaches for assessing lectin function. Lectins and their ligands are under quite a heavy microscope due to their potential applications to pharmacology, immunology, cancer therapy, and agriculture. With growing interest in the glycobiology field, the body of research related to lectin roles has grown at an explosive rate, particularly in the past 20 years. Animal Lectins: A Functional View presents the most up-to-date analysis of these carbohydrate-binding, and potentially lifesaving, proteins in one comprehensive volume. This standard-setting resource presents new insights into the biological roles of most animal lectins, including their function in infection prevention through innate immunity. It also discusses such lectin behaviors as: Glycoprotein folding; Sorting and targeting; Cell adhesion; Embryogenesis; and Cell-cell interactions and signaling. It describes the structural basis of protein-carbohydrate interactions. Under the editorial guidance ...
This resource presents information about animal lectins involved in various sugar-recognition processes. The resource is organised into two sections:. Part I: Structures and functions of animal lectins. Part II: C-type lectin-like domains. ...
Understanding the way lectins work is paramount to understanding the positive impact the Blood Type Diet can have on you. But before exploring how lectins behave within your body, you have to understand what a lectin actually is. In the simplest of terms, a lectin is a type of protein that acts as a selective Velcro-like material. They come in two main forms, single and double-sided. The single sided lectins only stick to other things. Cells in the liver have this type of lectin on their surfaces to snatch up harmful bacteria and parasites that may be present. Two sided lectins stick two other cells together, like a piece of double-sided tape between objects. Both varieties, despite their differences, have many commonalities. Although both the single and double sided kinds both cause agglutination (the clumping of particles), they do so in specific, individualized ways. Each lectin has a certain type of material it is meant to bind to and this changes depending on what species the
presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system. ...
Ficolin-3 is a protein that in humans is encoded by the FCN3 gene. Ficolin-3 was initially identified as H-ficolin, in which H is after the Hakata antigen that was previously found as an autoantigen in patients who lived in the city of Hakata. Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each ...
SHR # 960 - BEST OF - Lectins Investigated :: Guest: Krispin Sullivan :: Lectins can contribute to inflamation and disease. But now all Lectins are
Lectins are a family of glycoproteins (a complex protein containing a carbohydrate combined with a simple protein) found in the plant kingdom, including grains, legumes and solanacous plants (tomatoes, potatoes, eggplants and peppers)21, 27. Lectins also have the ability to bind sugar containing molecules. They were first studied for their ability to agglutinate (cause to adhere) red blood cells by binding to their cell membranes. Many lectins present in other foods are harmless, but some lectins found in grains, legumes and solanaceous plants have been shown to be harmful to human physiology28. Lectins are resistant to heat (unless cooked by pressure cooking)29 and digestive enzymes38, and therefore arrive intact when they reach the intestinal epithelium, passing through the intestinal barrier into peripheral circulation. Lectins are able to bind peripheral tissues, producing many deleterious health effects21. Furthermore, lectins disrupt intestinal barrier and immunological function when they ...
Principal Investigator:YAMAMOTO Kazuo, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Biological pharmacy
Bean sprouts arent just for hippies. Theres method in that madness -- reducing the harmful effects of lectins, substances found in many foods. If youre experiencing GI distress, lectins may be the culprit. Read more about these ubiquitous substances, and how to control their effects.
Hold onto your seats: Were bound to shake up everything you thought you knew about vegetables. You may be intrigued, or you might get upset, but lets let Dr. Steven R. Gundry explain.. Thirty years in the making, The Plant Paradox, Gundrys new book, is a trailblazing and revolutionary view on what foods aid in or debilitate your health. And a main culprit highlighted in the book is a little known protein called lectins. Lectins, as Gundry believes, are not only the cause of digestive upset, but also the root of all disease.. We chatted with Dr. Gundry, a trained cardiologist and heart surgeon, and had him highlight everything you need to know about lectins, and why you might want to cut them out of your diet.. In the simplest terms, what are lectins? And what do they do to our bodies?. Lectins are proteins that seek certain sugar molecules to bind to and impart or block information. Lectins are used by plants and animals as a main part of cellular communication. But plants use lectins as a ...
Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered
Lectins are proteins in plants designed to protect them from being eaten. Gluten is the best known lectin, but there are hundreds of them found in most plants, including many that we humans use for food. Lectins are not broken down in digestion and only partially destroyed by cooking ...
L-type lectins possess a luminal carbohydrate recognition domain (CRD) that binds to high-mannose-type oligosaccharides in aCa2+-dependent manner. The L-type CRD is named after the lectins found in...
Lectins are a new and important subject for me, thanks to a friend having shared a video about them recently. Now I want to share what information I have gathered regarding lectins with my readers in order to educated them. Especially those who are suffering gut problems when ingesting certain grains and legumes. This post…
Aberrant glycosylation occurs during the onset of the cancer and glycosylation patterns can provide information about different cancer types and metastatic potential. The work described here focuses on the development of both selective and cross-reactive boronic acid based synthetic lectins for recognizing cancer associated glycans and glycoproteins. Specifically, 1) optimizing library design and screening conditions, 2) identifying selective hits for proof-of-concept glycoproteins, 3) investigating how selective hits bind their respective targets, and 4) developing a sensor array to classify healthy and cancerous cells are discussed. The use of synthetic lectins holds great potential for applications in cancer diagnosis.
Macrophage galactose binding lectin (MGL) is the best studied of the multiple C-type lectins on macrophages [1][2]. It is also representative of the subclass of C-type lectins that bind galactose-related sugars. MGL consists of one CRD domain and contains cytoplasmic internalization motifs for endocytosis. No signaling properties have been described yet for MGL. Human MGL (CD301) and rat MGL are encoded by a single gene, whereas mice contain two MGL copies, mMGL-1 and mMGL-2 that differ in carbohydrate specificity [3][4][5]. ...
In this thesis, the syntheses of oligosaccharides for interaction studies with various lectins are described. The first section reports the syntheses of tetra, tri- and disaccharides corresponding to truncated versions of the glucosylated arm of Glc1Man9(GlcNAc)2, found in the biosynthesis of N-glycans. The thermodynamic parameters of their interaction with calreticulin, a lectin assisting and promoting the correct folding of newly synthesised glycoproteins, were established by isothermal titration calorimetry. In the second section, a new synthetic pathway leading to the same tetra- and trisaccharides is discussed. Adoption of a convergent strategy and of a different protecting group pattern resulted in significantly increased yields of the target structures. The third section describes the syntheses of a number of monodeoxy-trisaccharides related to the above trisaccharide Glc-α-(1→3)-Man-α-(1→2)-Man-α-OMe. Differentsynthetic approaches were explored and the choice of early introduction ...
Title: Medicinal Chemistry Based on the Sugar Code: Fundamentals of Lectinology and Experimental Strategies with Lectins as Targets. VOLUME: 7 ISSUE: 4. Author(s):Harold Rudiger, Hans-Christian Siebert, Dolores Solis, Jesus initial Jimenez-Barbero, Antonio Romero, Claus-Wilhelm von der Lieth, Teresa Diaz-Maurino and Hans-Joachim Gabius. Affiliation:Institut fur Pharmazie und Lebensmittelchemie, Julius-Maximilians-Universitat, Am Hubland, D-97074 Wurzburg, Germany. Keywords:lectinology, lectins as targets, molecular mechanics, computer assisted, NMR spectoscopic, crystallographic elucidation, sugar code, chemioal tailoring, phosphodiester backbone, microheterogeneity of glycan, monomer variability, N acetylneuramicinic, transgenic pollen, nitrogen enriched nutrients, rhizobium meliloti, non agglutinating ricin, hydrophobic molecules, phytopathogenic fungus, phosphomannose mutase, B bearing individuals, anti adhesion therapy, NMR spectrum, parenchymal host cells. Abstract: Theoretical calculations ...
Read "Comparative Characteristics of Carbohydrate Binding by Lectins from Broad Bean, Pea, Common Vetch, and Lentil Seeds, Russian Journal of Plant Physiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Flornes, Line Marie; Bryceson, Yenan T.; Spurkland, Anne; Lorentzen, JC; Dissen, Erik & Fossum, Sigbjørn (2004). Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex. Immunogenetics. ISSN 0093-7711. 56, s 506- 517 Vis sammendrag In an experimental rat model, we recently mapped an arthritis susceptibility locus to the distal part of Chromosome 4 containing genes predicted to encode C-type lectin superfamily (CLSF) receptors. Here we report the cDNA cloning and positional arrangement of these receptor genes, which represent rat orthologues to human Mincle and DCIR and to mouse MCL and Dectin-2, as well as four novel receptors DCIR2, DCIR3, DCIR4 and DCAR1, not previously reported in other species. We furthermore report the cDNA cloning of human Dectin-2 and MCL, and of the mouse orthologues to the novel rat receptors. Similar to the killer-cell lectin-like receptors (KLR) some of these receptors exhibit structural ...
Dr. Steven Gundrys book, The Plant Paradox: The Hidden Dangers in Healthy Foods That Cause Disease and Weight Gain, which spent 13 weeks on The New York Times best sellers list, reveals the deleterious effects of lectins on health. Ive...
Lectins are getting a lot of attention in the health and wellness community lately, but what are they exactly and what do they mean for our health? Read on t...
Mitogenic signals from concanavalin A and phytohemagglutinin were shown to undergo summation in time under conditions when each mitogen separately, if the duration of contact with lymphocytes during...
Glycoscience is an interdisciplinary field of research focused on understanding the structures and roles of sugar chains attached to proteins and lipids in biological systems. Every living cell is coated with complex sugars (glycans) and glycan-binding proteins (lectins). Interactions between glycans and lectins on adjacent cells are involved in many essential biological processes including cell differentiation, immunity, reproduction, signaling and diseases such as cancer, inflammation, microbial infections. Also, a large proportion of naturally occurring proteins that are secreted or are part of the extracellular matrix are glycosylated ...
I was wondering if you had any views on the health impact of lectins in food, assuming the food has been properly prepared. Ive had difficulty finding anything
Podoplanin (PDPN) is a unique transmembrane receptor that promotes tumor cell motility. cells within a few minutes of publicity, and inhibited individual OSCC dissemination in zebrafish embryos significantly. Furthermore, we survey that individual OSCC cells produced tumors that portrayed PDPN in rodents, and activated PDPN reflection in infiltrating web host murine cancers linked fibroblasts. Used jointly, these data recommend that lectins and antibodies might be utilized to combat OSCC and various other malignancies that sole PDPN. seedling lectin (MASL) can specifically focus on particular glycoproteins portrayed by individual cells [57, 58]. In reality, MASL, which provides a high affinity for antibody administration is normally complicated [48-50]. Unlike antibodies, lectins are resistant to gastrointestinal proteolysis [92-94], and can end up being used to deal with cancer tumor [56 orally, 93, 95]. In addition to carbohydrate adjustments, lectin connections are well guided by amino ...
摘要 利用Bac-to-Bac昆虫杆状病毒表达系统表达具有天然构象的猪Ficolin α。首先构建重组穿梭质粒rBacmid-Ficolin α,转染sf9细胞获得重组杆状病毒rBV-Ficolin α;通过Image J软件对优化表达的Western blot图片进行灰度分析确定较佳表达条件;纯化目的蛋白质后,进一步评价其体外抗病毒活性。Western blot结果显示以7.5 MOI接种量感染的sf9细胞在96 h时获得了较高的表达,同时获得的重组猪Ficolin α具有抗猪繁殖与呼吸综合征病毒(PRRSV)活性。猪Ficolin α在sf9昆虫细胞中获得成功表达,且具有良好的抗PRRSV活性。本研究可以为猪Ficolin α的抗病毒活性及作用机制研究提供物质基础。. ...
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Looking for online definition of C-type lectin superfamily member 12 in the Medical Dictionary? C-type lectin superfamily member 12 explanation free. What is C-type lectin superfamily member 12? Meaning of C-type lectin superfamily member 12 medical term. What does C-type lectin superfamily member 12 mean?
Purified Native Ricinus Communis Agglutinin I Protein, Rhodamine labeled from Creative Biomart. Native Ricinus Communis Agglutinin I Protein, Rhodamine labeled can be used for research.
Recombinant Aleuria aurantia lectin is produced in E.coli and has an amino acid sequence identical to native Aleuria aurantia lectin. AAL is a dimeric lectin with two identical subunits of approximately 36 kDa. Each subunit has five carbohydrate-binding sites. The lectin recognizes and binds specifically to fucose and terminal fucose residues on complex oligo saccharides and glycoconjugates. rAAL has binding affinity for fucose in all binding positions (alpha1-2, alpha1-3, alpha1-4 and alpha1-6) and in contrast to AAL purified from natural sources, rAAL is not contaminated with free fucose yielding higher affinity towards fucosylated oligosaccharides than native AAL. Recombinant AAL hemagglutinates erythrocytes irrespective of blood type (A, B and 0) at the same titers as AAL isolated from natural sources. AAL has been widely used for analysis and preparation of oligosaccharides and glycoproteins. Diagnostic applications include analysis of disease-associated glycosylation on plasma proteins.
Purified Native Aleuria Aurantia Lectin Protein from Creative Biomart. Native Aleuria Aurantia Lectin Protein can be used for research.
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Secreted Ectodomain of Sialic Acid-Binding Ig-like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage PolaritySecreted Ectodomain of Sialic Acid-Binding Ig-like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity ...
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BXXXX/B-2401-2.pdf]Anti-B Griffonia simplicifolia Le
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BXXXX/B-2402-2.pdf]Anti-Tk Griffonia simplicifolia L
Griffonia simplicifolia is a coarse aggressive brier built-in to West Africa and Central Africa. It grows to about 3 m, and bears greenish flowers followed by atramentous pods. The seeds of the bulb are acclimated as an herbal supplement for their 5-hydroxytryptophan (5-HTP ) content. 5-Hydroxytryptophan is an important architecture block for the animal physique…
Sialic acid-binding Ig-like lectin-7 (Siglec-7) expression is strongly reduced on natural killer (NK) cells from HIV-1 infected viremic patients. To investigate the mechanism(s) underlying this phenomenon, we hypothesized that Siglec-7 could contribute to the infection of CD4pos target cells following its interaction with HIV-1 envelope (Env) glycoprotein 120 (gp120). The ability of Siglec-7 to bind gp120 Env in a sialic acid-dependent manner facilitates the infection of both T cells and monocyte-derived macrophages (MDMs). Indeed, pre-incubation of HIV-1 with soluble Siglec-7 (sSiglec-7) increases the infection rate of CD4pos T cells, which do not constitutively express Siglec-7. Conversely, selective blockade of Siglec-7 markedly reduces the degree of HIV-1 infection in Siglec-7pos MDMs. Finally, the sSiglec-7 amount is increased in the serum of AIDS patients with high levels of HIV-1 viremia and inversely correlates with CD4pos T cell counts. Our results show that Siglec-7 binds HIV-1 and contributes
A number of genes encoding C-type lectin molecules have been mapped to the natural killer gene complex (NKC) at the distal region of mouse chromosome 6 and to a syntenic region on human chromosome 12p12-p13. In addition to those receptors which regulate NK cell function, related structures expressed on other cells types have also been localized to this chromosomal region. Among these are a number of recently characterized genes, including macrophage C-type lectin (MCL), macrophage-inducible C-type lectin (Mincle), dendritic cell immunoreceptor (DCIR) and dendritic cell-associated lectin-2 (Dectin-2). The amino acid sequences comprising the single C-type lectin domains of MCL, Mincle, DCIR and Dectin-2 are shown here to be closely related to each other. These molecules show overall similarity to two groups of animal C-type lectins, groups II and V, which demonstrate type II transmembrane topology. In this study, sequence analysis suggests that MCL, Mincle, DCIR and Dectin-2 represent a subset of group II
GSL II is affinity purified tetramer that contains a single type of 30 kDa subunit. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.This lectin has a carbohydrate specificity for αGal and αGalNAc and elutes with galactose or N-acetylgalactosamine. Incr
Several lectins recognize n-acetyl-glucosamine in a glycoprotein. Based on the linkage and specificity for binding, different N-Acetyl-Glucosamine-binding lectins are utilized to obtain optimum results.
Lectins were defined as proteins that recognize specific carbohydrate structures and agglutinate cells by binding to cell surface glycoproteins and glycoconjugates. Many kinds of different lectins have been purified from fish eggs. Lectins may have a variety of biological function in fish eggs, such as block of polyspermy, regulation of carbohydrates metabolism, participation in the formation of fertilization envelope after binding with glycoproteins, antibacterial effect and opsonization of pathogens. Therefore, lectins are important components in oocytes and play important roles. Cortical granules were as a kind of secretory vesicles that unique to oocyte. They are synthesized and accumulated during oogenesis, translocated to the cell surface before fertilization and exocytosed after fertilization. Cortical granule contents have diverse components, such as proteases and lectins, and play important roles in egg fertilization and early embryogenesis.Gibel carp oocyte-specific lectin (GOL) had ...
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Do you suffer from symptoms after eating? Such as digestive symptoms, foggy brain, sinus congestion or aches and pains. Do you notice it after gluten containing foods or just cant track whats causing it? Chances are youre body is reacting to components in plants called lectins.. What are lectins? So happy you asked!. Lectins are the plants natural compounds to ward off pests, fungal and bacterial attack. That is, the plants natural immune system. When plants are under attack they raise their lectin numbers to fend off the attacking pests. This is the system by which pesticides work. However, pests are gaining tolerance resulting in needing an increase of pesticide to be applied to the plant and thus a further increase of the plants lectins.. Modern wheat strains vs old style grains. Our modern wheat strains have been modified to produce more lectins to be highly pest resistant. This very clever genetically engineering won Norman Borlaug a Nobel peace prize in 1970 and changed the industry ...