Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical study, Sara Amini, Mohammad Reza Nikravesh, Mehdi Jal

Humans and mice with deficiency of the alpha2 subunit of the basement membrane protein laminin-2/merosin suffer from merosin-deficient congenital muscular dystrophy (MCMD). We have expressed a human laminin alpha2 chain transgene under the regulation of a muscle-specific creatine kinase promoter in …

in Journal of the National Cancer Institute (1992), 84(15), 1161-9. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin ... [more ▼]. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin-binding proteins, including the 67-kd laminin receptor (67 LR) and a 31-kd human laminin-binding protein (HLBP31) homologous to the 31-kd human IgE-binding protein/galactoside-binding lectin. PURPOSE: To investigate whether various laminin-binding proteins are differentially expressed in human colon carcinoma, we studied messenger RNA (mRNA) levels of the 67 LR and HLBP31 in matched tumor and adjacent normal mucosa samples from a series of 21 patients. METHODS: Total cellular RNA from tumor and normal mucosa was isolated ...

Schwann cell basement membranes do not appear to form in developing tissues until the stage of neuronal ensheathment, and it has been postulated that axonal contact triggers their assembly, in turn affecting cell polarization, ensheathment and myelination ( Clark and Bunge, 1989; Jaakkola et al., 1993). The data presented in this study suggest that Schwann cell basement membrane assembly is a process that first requires the development of cell surface competency for laminin binding, followed by the binding and assembly of laminin. We consider this ECM as a `nascent basement membrane (i.e. a laminin-based ECM with classical ultrastructural morphology of a basement membrane, but still lacking in type IV collagen). The nascent basement membrane is likely to be no more than one laminin molecular layer thick. Upon exposure of the cells to type IV collagen, this component is incorporated into the laminin matrix. In Schwann cells, the laminin binding/assembly step depends in part upon an interaction ...

Korpos et al. (1) claim that limited information exists on the nature of the ECM [extracellular matrix] of the pancreas and, in particular, on the composition of peri-islet capsule, and that they provide the the first comprehensive analysis of the [ECM] composition of peri-islet capsules.. We dispute the strength of these claims. In mice we originally reported in 2008 that a basement membrane (BM) exists around islets, and eight unique components were identified (type IV collagens α1, α2, laminin α2, β1, γ1, nidogen 1, nidogen 2, perlecan) as well as two composite components (laminin Engelbreth-Holm-Swarm, collagen IV) (2). It was also shown that type IV collagens α3, α4, α5, α6 were not present (2). Korpos et al. (1) were able to find a further three unique components (laminin α4, β2, agrin) and one composite component (laminin 322).. In 2008 in humans (3,4), at least four unique components (laminin α1, α5, β1, γ1) were identified and in their Supplementary Figure 1 staining ...

TY - JOUR. T1 - Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups. AU - Kostrominova, Tatiana. AU - Tanzer, M. L.. PY - 1995. Y1 - 1995. N2 - Laminin glycosyl groups are necessary for the spreading of murine melanoma cells which become attached to this glycoprotein. Laminin has been implicated in myogenesis but the potential role of its glycosyl groups in this process has not been examined. In this study we report the effects of the carbohydrate moieties of laminin on myoblast adhesion, spreading, and differentiation. Unglycosylated laminin from tunicamycin-treated cultures of a mouse cell line, M1536 B3, was used in the experiments. Glycosylated laminin from a murine tumor and from cultures of M1563 B3 cells served as controls. Cell binding experiments with C2C12 mouse myoblasts showed that the cells preferred a laminin-coated surface, compared to the uncoated plastic surface (nontissue culture wells). Myoblasts did not distinguish ...

The disulfide bonding patterns in the N-terminal (LN) domains of the basement membrane protein laminin beta 1 have not been investigated so far. We report an in-depth mass spectrometric analysis using offline nano-high-performance liquid chromatography/matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (nano-HPLC/MALDI-TOF/TOF-MS) for determining the disulfide bond patterns in the LN-domain of recombinant mouse laminin beta 1 chain for the first time. Mass spectra were recorded and the putatively disulfide-linked peptides were subjected to LIFT-TOF/TOF-MS to confirm the disulfide bond. Screening the fragment ion mass spectra of disulfide-linked peptides for characteristic 66-amu patterns (34 u +32 u), arising from symmetric and asymmetric cleavage of disulfide bonds, facilitated their identification. Using various enzymes for proteolytic digestion of a recombinant laminin beta 1 chain N-terminal protein fragment, a linear bonding pattern of the eight ...

The human non-integrin laminin receptor (LamR) is a multifunctional protein which is localised to a number of sub-cellular locations. LamR is a component of the ribosome and has a number of intracellular functions; it also acts as an extracellular receptor for laminin, growth factors, pathogenic microorganisms, prion proteins, toxins and the anticarcinogen epigallocatechin gallate (ECGC). Although LamR is present in most cellular compartments, its overexpression in many types of cancer cells suggests a vital role for LamR in tumor-cell migration and invasion. There are two isoforms of laminin receptor: the monomeric 37-kDa laminin receptor precursor (37LRP) and the mature 67-kDa laminin receptor (67LR). Although the precise molecular nature of 67LR is unclear, accumulating evidence strongly suggest that 37LRP can undergo homo- and/or hetero-dimerization with Galectin-3 (Gal-3) to form mature 67LR. A recent study demonstrated that both homo- and heterodimer LamR forms are present on the cell ...

Laminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell shape change and are organized into an epithelium. Laminin is present on the basal surfaces of ectodermal cells and is absent or reduced on migrating primary mesenchyme cells. Microinjection of a monoclonal antibody directed against laminin induces a morphological change in cell shape and a deformation of the embryonic epithelium. Investigation of selected stages of live embryos suggests that the distribution of laminin may be heterogeneous within the basal lamina during early development. The results implicate laminin as a mediator of cell shape change during early morphogenesis.. ...

Institutions: 1) University of Nottingham, 2) University of Vienna. The myodystrophy mouse is a spontaneous, autosomal recessive model of muscular dystrophy. We previously identified the molecular defect underlying the myodystrophy (Largemyd) mouse as a deletion in the Large gene, encoding a glycosyltransferase. This mutant is currently the only animal model for studying the role of glycosylation in the group of human muscle disorders due to mutated glycosyltransferases.Dystroglycan, an extensively modified glycoprotein, is a central component of the dystrophin-associated glycoprotein complex; this plays an important role in maintaining muscle membrane integrity. Furthermore, glycosylation of ?lpha?dystroglycan is necessary for its interaction with the extracellular matrix protein laminin. Western analyses of Largemyd proteins reveal hypo-glycosylation of alpha-dystroglycan and loss of laminin binding. We are currently using the glycan-binding specificities of lectins to investigate the overall ...

in Invasion & Metastasis (1993), 13(1), 1-30. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several ...

HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show that competency for differentiation is due at least in part to the modulation of extracellular matrix components. In particular we have studied laminin and tenascin. EGF alters endogenous laminin assembly. In addition, promotion of competency can be partially mimicked by plating HC11 cells on the E8 laminin fragment, which is able to induce lactogenic responsiveness in cells grown in the absence of EGF or bFGF. The production and assembly of tenascin is also dependent upon the growth conditions of the HC11 cells. EGF- or bFGF-grown competent cells produce tenascin but do not assemble it at the extracellular matrix as efficiently as Ins- or PDGF-grown, non-competent cells. This ...

Macrophages require activation with either PMA (Mercurio, A. M., and L. M. Shaw. 1988. J. Cell Biol. 107:1873-1880) or interferon-gamma (Shaw, L. M., and A. M. Mercurio. 1989. J. Exp. Med. 169:303-308) to adhere to a laminin substratum. In the present study, we identified an integrin laminin receptor on macrophages and characterized cellular changes that occur in response to PMA activation that facilitate laminin adhesion. A monoclonal antibody (GoH3) that recognizes the integrin alpha 6 subunit (Sonnenberg, A., H. Janssen, F. Hogervorst, J. Calafat, and J. Hilgers. 1987. J. Biol. Chem. 262:10376-10383) specifically inhibited adhesion to laminin-coated surfaces. This antibody precipitated an alpha 6 beta 1 heterodimer (Mr 130/110 kD) from 125I surface-labeled macrophages. The amount of radiolabeled receptor on the cell surface did not increase after PMA activation. Thus, the induction of laminin adhesion cannot be attributed to de novo or increased surface expression of alpha 6 beta 1. By ...

TY - JOUR. T1 - Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle. AU - Gulati, A. K.. AU - Reddi, A Hari. AU - Zalewski, A. A.. PY - 1984. Y1 - 1984. N2 - Extracellular matrix is known to play an important role during development and maintenance of various tissues. In the present study, changes in two extracellular matrix glycoproteins, fibronec ?ptin and laminin, were investigated in skeletal muscle undergoing immune rejection. Purified antibodies against fibronectin and laminin were used to analyze the matrix by indirect immunofluorescence at various intervals after transplantation of extensor digitorum muscle in rats. Fibronectin and laminin were localized in the pericellular basement membrane zone of the normal myofibers; however, the cytoplasm was devoid of both glycoproteins. Transplanted muscle grafts underwent a process of degeneration and then an initial regeneration during the first 7 days. This regeneration effort ceased ...

Looking for online definition of laminin, alpha 4 in the Medical Dictionary? laminin, alpha 4 explanation free. What is laminin, alpha 4? Meaning of laminin, alpha 4 medical term. What does laminin, alpha 4 mean?

In humans, mutations in the lama2 gene, which encodes laminin a2, cause merosin-deficient congenital muscular dystrophy 1A MDC1A , one subset of a broad

The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basement membrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton. We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted ...

Endothelial basement membranes constitute barriers to extravasating leukocytes during inflammation, a process where laminin isoforms define sites of leukocyte exit; however, how this occurs is poorly understood. In addition to a direct effect on leukocyte transmigration, we show that laminin 511 affects endothelial barrier function by stabilizing VE-cadherin at junctions and downregulating expression of CD99L2, correlating with reduced neutrophil extravasation. Binding of endothelial cells to laminin 511, but not laminin 411 or non-endothelial laminin 111, enhanced transendothelial cell electrical resistance (TEER) and inhibited neutrophil transmigration. Data suggest that endothelial adhesion to laminin 511 via beta1 and beta3 integrins mediates RhoA-induced VE-cadherin localization to cell-cell borders, and while CD99L2 downregulation requires integrin beta1, it is RhoA-independent. Our data demonstrate that molecular information provided by basement membrane laminin 511 affects leukocyte ...

The alpha 6 beta 1 integrin is expressed on the macrophage surface in an inactive state and requires cellular activation with PMA or cytokines to function as a laminin receptor (Shaw, L. M., J. M. Messier, and A. M. Mercurio. 1990. J. Cell Biol. 110:2167-2174). In the present study, the role of the alpha 6 subunit cytoplasmic domain in alpha 6 beta 1 integrin activation was examined. The use of P388D1 cells, an alpha 6-integrin deficient macrophage cell line, facilitated this analysis because expression of either the alpha 6A or alpha 6B subunit cDNAs restores their activation responsive laminin adhesion (Shaw, L. S., M. Lotz, and A. M. Mercurio. 1993. J. Biol. Chem. 268:11401-11408). A truncated alpha 6 cDNA, alpha 6-delta CYT, was constructed in which the human cytoplasmic domain sequence was deleted after the GFFKR pentapeptide. Expression of this cDNA in P388D1 cells resulted in the surface expression of a chimeric alpha 6-delta CYT beta 1 integrin that was unable to mediate laminin adhesion ...

Neuromuscular junction (NMJ) assembly is characterized by the clustering and neuronal alignment of acetylcholine receptors (AChRs). In this study we have addressed post-synaptic contributions to assembly that may arise from the NMJ basement membrane with cultured myotubes. We show that the cell surface-binding LG domains of non-neural (muscle) agrin and perlecan promote AChR clustering in the presence of laminin-2. This type of AChR clustering occurs with a several hour lag, requires muscle-specific kinase (MuSK), and is accompanied by tyrosine phosphorylation of MuSK and betaAChR. It also requires conjugation of the agrin or perlecan to laminin together with laminin polymerization. Furthermore, AChR clustering can be mimicked with antibody binding to non-neural agrin, supporting a mechanism of ligand aggregation. Neural agrin, in addition to its unique ability to cluster AChRs through its B/z sequence insert, also exhibits laminin-dependent AChR clustering, the latter enhancing and stabilizing ...

Objective:To investigate the effects of puerarin on renal structure?function and expressions of MMP-3 and TIMP-1 in the kidney of diabetic rats.Methods:Uninephrectomized STZ-induced male wister rats were used.Puerarin was delivered daily by the intraperitoneal injection from the third day of induction to diabetes for 16 weeks.Using in situ hybridization and immunohistochemistry to detect TIMP-1mRNA expression and MMP-3?TIMP-1?collagen Ⅳ and Laminin expressions in diabetic kidneys with image analysis system;Flow cytometry was used to detect the expressions of MMP-3?TIMP-1.Results:BUN?Ccr and Upro in diabetic rats were significant higher than that in control group.Compared with those in kidneys of control group,diabetic kidneys showed increased expressions of both MMP-3?TIMP-1 proteins and excessive deposition of glomeruli extracellular matrix(P0.05).The blood glucose and the level of expressions of MMP-3?TIMP-1?collagen Ⅳ and Laminin were reduced by puerarin treatment,renal function was improved

Laminin Receptor antibody LS-C197601 is an unconjugated mouse monoclonal antibody to human Laminin Receptor (RPSA). Validated for ELISA and WB.

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In previous studies, researchers at the Cells-in-Motion Cluster of Excellence had discovered that specific proteins in the basement membrane - so-called laminins - influence the migration of the immune cells. For example, they determine the place at which immune cells cross the basement membrane and enter into the tissue. Researchers have now discovered that these components of the basement membrane also affect the inner endothelial cell layer.. A certain laminin - laminin 511 - influences how tightly endothelial cells are connected to each other, explains biochemist Prof. Lydia Sorokin. This is because laminin affects specific molecules that interconnect endothelial cells. For one thing, laminin stabilizes the VE-cadherin molecule at contact sites between adjacent endothelial cells, forming a sort of Velcro fastener between them. In addition, laminin 511 reduces the quantity of a molecule known as CD99L2.. This is found on both the endothelial cells and on the immune cells and permits the ...

Mokkapati, Sharada (2007) Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin. [Thesis Abstract]. ...

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture pla

TY - JOUR. T1 - Decreased expression of galectin-3 is associated with progression of human breast cancer. AU - Castronovo, Vincent. AU - Van den Brûle, Frédéric A.. AU - Jackers, Pascale. AU - Clausse, Nathalie. AU - Liu, Fu-Tong. AU - Gillet, Claudette. AU - Sobel, Mark E.. PY - 1996. Y1 - 1996. N2 - Galectin-3, a member of the β-galactoside-binding lectin family, is involved in several biological events including binding to the basement membrane glycoprotein laminin. Although the exact role of galectin-3 during the interactions between cells and laminin is not yet known, it has recently been observed that its expression is down-regulated at both the protein and the mRNA level in colon cancer tissues in correlation with progression of the disease. This study investigated the possibility that breast cancer cells might also exhibit decreased galectin-3 expression in association with their aggressiveness. The expression of galectin-3 was examined by immunoperoxidase staining, using a ...

The basement membrane (BM) is a specialized form of extracellular matrix (ECM) underlying epithelia and endothelia and surrounding many types of mesenchymal cells. Nidogen, along with collagen IV and laminin, is a major component of BMs. Although certain ECM proteins such as laminin or reelin influence neuronal function via interactions with cell-surface receptors such as integrins, behavioral neurological impairments due to deficits of BM components have been recognized only recently. Here, alterations in neuronal network function underlying these behavioral changes are revealed. Using nidogen-1 knockout mice, with or without additional heterozygous nidogen-2 knockout (NID1(-/-)/NID2(+/+) or NID1(-/-)/NID2(+/-)), we demonstrate that nidogen is essential for normal neuronal network excitability and plasticity. In nidogen-1 knockouts, seizurelike behavior occurs, and epileptiform spiking was seen in hippocampal in vivo EEG recordings. In vitro, hippocampal field potential recordings revealed that ...

The capability of the integrin VLA-3 to function as a receptor for collagen (Coll), laminin (Lm), and fibronectin (Fn) was addressed using both whole cell adhesion assays and ligand affinity columns. Analysis of VLA-3-mediated cell adhesion was facilitated by the use of a small cell lung carcinoma line (NCI-H69), which expresses VLA-3 but few other integrins. While VLA-3 interaction with Fn was often low or undetectable in cells having both VLA-3 and VLA-5, NCI-H69 cells readily attached to Fn in a VLA-3-dependent manner. Both Arg-Gly-Asp (RGD) peptide inhibition studies, and Fn fragment affinity columns suggested that VLA-3, like VLA-5, may bind to the RGD site in human Fn. However, unlike Fn, both Coll and Lm supported VLA-3-mediated adhesion that was not inhibited by RGD peptide, and was totally unaffected by the presence of VLA-5. In addition, VLA-3-mediated binding to Fn was low in the presence of Ca++, but was increased 6.6-fold with Mg++, and 30-fold in the presence of Mn++. In contrast, ...

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Stem cell culture is accompanied by extra challenges, and it is critical to maintain genetic stability, ensure stemness, and prevent spontaneous differentiation. Cellartis media and supplements are optimized for derivation, maintenance, and expansion of pluripotent cells.. The Cellartis DEF-CS system is a complete culture system for robust expansion of human iPS cells in a feeder-free and defined environment. A xeno-free version is also available.. iMatrix-511 is a xeno-free recombinant laminin fragment for ES or iPS cell culture substrate; iMatrix-511 provides greater adhesive properties than full-length laminin, vitronectin, or reagents containing an undefined mixture of basement membrane.. ...

Integrin alpha6beta1: A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.

Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…

Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…

A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...

A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...

Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Additional information: Clone REA276 displays negligible binding to Fc receptors.

Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease.Additional information: Clone REA276 displays negligible binding to Fc receptors.

Background and Objective: It is obviously approved that Fibronectin and Laminin α-5 (LAMA5) expression will change, in some organs of fetuses that their mothers were smoking during pregnancy period. For example, the expression of mentioned proteins in newborns decreases in parenchyma of their lungs. In present study, we evaluated the effects ...

Brain-specific homing and direct interactions with the neural substance are prominent hypotheses for brain metastasis formation and a modern manifestation of Pagets seed and soil concept. However, there is little direct evidence for this neurotropic growth in vivo. In contrast, many experimental studies have anecdotally noted the propensity of metastatic cells to grow along the exterior of pre-existing vessels of the CNS, a process termed vascular cooption. These observations suggest the soil for malignant cells in the CNS may well be vascular, rather than neuronal. We used in vivo experimental models of brain metastasis and analysis of human clinical specimens to test this hypothesis. Indeed, over 95% of early micrometastases examined demonstrated vascular cooption with little evidence for isolated neurotropic growth. This vessel interaction was adhesive in nature implicating the vascular basement membrane (VBM) as the active substrate for tumor cell growth in the brain. Accordingly, VBM

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Science Meets God Many of you are aware of my background in Exercise and Nutritional Sciences, here is where Science meets God. Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. The laminins are an important and biologically active…

Business Owner Linda Morris published a press release titled: The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..

Business Owner Linda Morris published a press release titled: Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?

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We have isolated and characterized the expression of a cDNA clone from the sea urchin Strongylocentrotus purpuratus that encodes a protein very similar to LBP/p40, originally identified as a nonintegrin, 67-kDa laminin binding protein. The deduced amino acid sequence of the protein, which we call spLBP/p40, shows significant similarity with the LBP/p40 from other sources, although significant divergence does occur at the carboxyl end. The S. purpuratus mRNA is present as a maternal transcript and its level remains constant until activation of zygotic transcription at the hatching blastula stage, whereupon the total spLBP/p40 increases through the pluteus larval stage. Adult tissues also contain the spLBP/p40 mRNA. Both maternal and zygotic transcripts are translated as determined by their presence in polysomes. Immunoblot analysis using an antibody raised against a recombinant fusion protein indicates that the concentration of the spLBP/p40 protein remains constant during development despite the

Extracellular Matrix Protein Laminin Enhances Mesenchymal Stem Cell (MSC) Paracrine Function through αvβ3/CD61 Integrin to Reduce Cardiomyocyte Apoptosis Researchers found that placenta-derived multipotent cells (PDMCs) significantly reduced cardiomyocyte apoptosis and reactive oxygen species production through the paracrine factors GRO-α, HGF and IL-8. The enhancement of PDMC paracrine function by laminin was mediated through αvβ3 integrin, with involvement of the signaling pathways of JNK, for GRO-α and IL-8 secretion, and PI3K/AKT, for HGF secretion. [J Cell Mol Med] Full Article Transplantation of Human Bone Marrow Mesenchymal Stromal Cells Reduces Liver Fibrosis More Effectively than Whartons Jelly Mesenchymal Stromal Cells The authors compared the relative therapeutic potential of pooled, adult human bone marrow (BM)- and neonatal Whartons jelly-derived mesenchymal stromal cells (MSCs) to treat CCl4-induced liver fibrosis in rats. BM-MSCs treatment showed a marked reduction in ...

TY - JOUR. T1 - Silencing GFAP isoforms in astrocytoma cells disturbs laminin-dependent motility and cell adhesion. AU - Moeton, M. AU - Kanski, R. AU - Stassen, Oscar. AU - Sluijs, JA. AU - Geerts, D. AU - Tijn P, van. AU - Wiche, G. AU - Strien ME, van. AU - Hol, EM. PY - 2014. Y1 - 2014. N2 - Glial fibrillary acidic protein (GFAP) is an intermediate filament protein expressed in astrocytes and neural stem cells. The GFAP gene is alternatively spliced, and expression of GFAP is highly regulated during development, on brain damage, and in neurodegenerative diseases. GFAP alpha is the canonical splice variant and is expressed in all GFAP-positive cells. In the human brain, the alternatively spliced transcript GFAP alpha marks specialized astrocyte populations, such as subpial astrocytes and the neurogenic astrocytes in the human subventricular zone. We here show that shifting the GFAP isoform ratio in favor of GFAP alpha in astrocytoma cells, by selectively silencing the canonical isoform GFAP ...

TY - JOUR. T1 - Immunohistochemical Localization of Laminin and Cytokeratin in Embryonic Alligator Gonads. AU - Smith, Craig A.. AU - Joss, Jean M.P.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - Gonadal sex differentiation is temperature‐dependent in Alligator mississippiensis; testis differentiation occurs in embryos incubated at 33°C and ovary differentiation occurs in embryos incubated at 30°C. Laminin and cytokeratin were examined immunohistochemically in the gonads of alligator embryos incubated at these temperatures. The aim of this study was to determine whether these structural proteins show the same sex‐specific expression patterns reported for mammalian embryos, and to assess their usefulness as early markers of gonadal differentiation in species with temperature‐dependent sex determination. Laminin delineated enlarged seminiferous cords in differentiating testes from developmental stage 23 to hatching. Laminin distribution was more diffuse and revealed smaller cords of cells in ...

Here we describe extracellular matrix alterations in footpad lesions and draining lymph nodes caused by Leishmania (L.) amazonensis in mouse strains with distinct susceptibilities to this parasite: BALB/c (susceptible), C57BL/6 (intermediate), and DBA/2 (resistant). Changes in ECM were observed mainly in BALB/c mice that, in general, presented tissue damage associated with high parasite burden. Under polarized light, Sirius Red revealed type I collagen that was predominant in the primary lesion in all strains studied at the early phase of infection, but gradually decreased and was replaced by abundant type III collagen fibre in chronic phase lesions. the presence of type III collagen seemed to provide support to inflammatory cells, mainly vacuolated and parasitized macrophages. Laminin expression was not altered during infection by L. (L.) amazonensis in any of the mouse strains studied. Furthermore, the decreased fibronectin expression, in all strains, in areas where amastigotes have been ...

Autoantibodies to diverse antigens escape regulation in systemic lupus erythematosus under the influence of a multitude of predisposing genes. NZB mice based on Docosanol absence of serum transgenic anti-laminin autoantibodies and failure to Docosanol recover spontaneous anti-laminin monoclonal antibodies. Four- to six-fold depletion of splenic B cells in transgenic mice of these strains as well as in MRL/lpr transgenic mice and reduced frequency of HBEGF IgM+ bone marrow B cells suggest that central deletion is usually grossly intact. Nonetheless the four strains demonstrate unique transgenic B cell phenotypes including endotoxin-stimulated production of anti-laminin antibodies by B cells from transgenic NZB mice and in vitro hyperproliferation of both endotoxin- and BCR-stimulated B cells from transgenic BXSB mice which are shown to have an enrichment of CD21-high marginal zone cells. Rare anti-laminin transgenic B cells spontaneously escape tolerance in MRL/lpr mice. Further study of the ...

It has been well documented that the extracellular matrix components fibronectin and laminin promote or regulate morphogenesis of the myocardial cells in mammalian heart. However, their chronological change of expression (or localization) in the human heart remains elusive. In this study, fibronectin and laminin in the left ventricle of forty-two human fetuses aged from 8 to 26 weeks gestation and left ventricular tissues obtained from a 2-week old infant and two adults were investigated by Western blot analyses and indirect immunofluorescence technique with monoclonal antibodies. In the fetal heart, fibronectins were present along the endocardium, epicardium, and linings of larger blood vessels. In 14-16 weeks gestation, fibronectin immunofluorescence became stronger but not evenly dispersed in the interstitium. After 24 weeks gestation, they were strongly positive only in the relatively larger blood vessels, as well as those in the infant and adult cardiac tissues. Laminins were strongly positive

Laminins form a large family of extracellular matrix (ECM) proteins, and their expression is a prerequisite for normal embryonic development. Herein we investigated the role of the laminin gamma1 chain for cardiac muscle differentiation and function using cardiomyocytes derived from embryonic stem c …

TY - JOUR. T1 - A comparison of fibronectin, laminin, and galactosyltransferase adhesion mechanisms during embryonic cardiac mesenchymal cell migration in vitro. AU - Loeber, Cleo P.. AU - Runyan, Raymond B.. PY - 1990/8. Y1 - 1990/8. N2 - Embryonic hearts contain a homogeneous population of mesenchymal cells which migrate through an extensive extracellular matrix (ECM) to become the earliest progenitors of the cardiac valves. Since these cells normally migrate through an ECM containing several adhesion substrates, this study was undertaken to examine and compare three ECM binding mechanisms for mesenchymal cell migration in an in vitro model. Receptor mechanisms for the ECM glycoproteins fibronectin (FN) and laminin (LM) and the cell surface receptor galactosyltransferase (GalTase), which binds an uncharacterized ECM substrate, were compared. Primary cardiac explants from stage 17 chick embryos were cultured on three-dimensional collagen gels. Mesenchymal cell outgrowth was recorded every 24 hr ...

Previous work has demonstrated that loss of crucial components of the zebrafish MTJ, either intracellularly (dystrophin, integrin-linked kinase) or extracellularly (Lama2), results in an embryonic myofibre detachment phenotype (Bassett et al., 2003; Hall et al., 2007; Postel et al., 2008). In this study we extend our knowledge of the role of another extracellular component, Lamb2, by showing that a disorganised MTJ, detachment of muscle fibres and sarcolemmal damage all result from a missense mutation in the zebrafish lamb2 gene in the softm272a mutant. Whereas secondary changes in levels of LAMB2 immunoreactivity have been seen in congenital muscular dystrophy, this laminin chain has not been implicated as a direct cause of inherited muscle disease (Cohn et al., 1997). Rather, mutations in LAMB2 cause Pierson syndrome, an autosomal recessive, oculorenal disease with neurological involvement (Zenker et al., 2004). No dystrophy has been reported in Pierson syndrome; however, deletion of Lamb2 in ...

TY - JOUR. T1 - Temporal and spatial appearance of α-dystroglycan in differentiated mouse myoblasts in culture. AU - Kostrominova, Tatiana. AU - Tanzer, M. L.. PY - 1995. Y1 - 1995. N2 - The dystrophin-glycoprotein complex plays an important role in muscle function. One of the components of the complex, a 156-kDa cell surface glycoprotein (α-dystroglycan) binds to laminin, thereby connecting the basal lamina and muscle cells. We have examined the progressive appearance of α- dystroglycan and laminin in muscle cells that differentiate in culture. We find that nondifferentiated cultures of C2C12 myoblasts express low amounts of dystroglycan mRNA and, in contrast, this gene is prominently expressed in differentiated myotubes. Immunofluorescence analysis with a monoclonal antibody against α-dystroglycan shows its progressive appearance during myoblast differentiation into myotubes. Immunostaining with a monoclonal antibody against laminin shows that it is not present on the surface of ...

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rat calsequestrin protein: amino acid sequence given in first source; MW 56-66 kDa; a major skeletal muscle laminin binding protein

The 96 Well 3D Spheroid BME Cell Invasion Assay offers a standardized, three dimensional, high content format for quantitating the degree to which invasive cells penetrate a barrier, consisting of basement membrane components, in vitro in response to chemoattractants and/or inhibiting compounds, which is fundamental for angiogenesis1, embryonic development2, immune response3, and tumor cell metastasis4. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. They not only support cells and cell layers, but they also play an essential role in tissue organization that influences cell adhesion, migration, proliferation, and differentiation. Basement membranes are degraded and regenerated during development and wound repair, and they are major barriers to invasion by metastatic tumor cells. Current methods for assessing cell invasion through basement membrane barrier most ...

Elias, M. C. Q. B., Chammas, R., Giorgi, R. R., Frochtengarten, F., Santos, C. L. S., & Brentani, R. R. (1994). Adhesion to laminin is down-regulated upon retinoic acid-induced f9 cell differentiation: a role for alpha 6 / beta 1 integrin. Brazilian Journal of Medical and Biological Research. Ribeirao Preto ...

The precise relationship of embryonic stem cells (ESCs) to cells in the mouse embryo remains controversial. We present transcriptional and functional data to identify the embryonic counterpart of ESCs. Marker profiling shows that ESCs are distinct from early inner cell mass (ICM) and closely resemble pre-implantation epiblast. A characteristic feature of mouse ESCs is propagation without ERK signalling. Single-cell culture reveals that cell-autonomous capacity to thrive when the ERK pathway is inhibited arises late during blastocyst development and is lost after implantation. The frequency of deriving clonal ESC lines suggests that all E4.5 epiblast cells can become ESCs. We further show that ICM cells from early blastocysts can progress to ERK independence if provided with a specific laminin substrate. These findings suggest that formation of the epiblast coincides with competence for ERK-independent self-renewal in vitro and consequent propagation as ESC lines. It has been unclear at which stage of

Previous studies demonstrated the cross antigenicity between endothelial and epithelial basement membranes. In the present study we have isolated and characterized chemically and immunologically the antigenic components of basement membranes from the glomerulus, alveolus, and lens capsule. The basement membranes were solubilized by reduction and alkylation and antisera prepared in rabbits. On immunodiffusion two precipitin lines of identity form when antiserum to either reduced and alkylated glomerulus or lens-capsule basement membranes is reacted with both antigens. Reduced and alkylated alveolus basement membrane reacts similarly with both antisera. Upon fractionation of the basement membranes two antigenic components are obtained. One is ...

Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. ...

http://www.youtube.com/watch?v=iYFHQzLp4fM The story about the Laminin protein being a sign from God to humanity has been circulating the internet, circa 2000. The above attached is a popular video, currently enjoying 3.7 million hits on YouTube. The presentation initially starts out containing a powerful scripture message read by Louie. If he had stopped there, the edifying significance would have been exceptional… but… As you proceed to view the video, Louies transitions to an

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Nidogen-2, also known as osteonidogen, is a basal lamina protein of the nidogen family. It was the second nidogen to be described after nidogen-1 (entactin). Both play key roles during late embryonic development. In humans it is encoded by the NID2 gene. GRCh38: Ensembl release 89: ENSG00000087303 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000021806 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Miosge N, Holzhausen S, Zelent C, Sprysch P, Herken R (2002). Nidogen-1 and nidogen-2 are found in basement membranes during human embryonic development. The Histochemical Journal. 33 (9-10): 523-30. doi:10.1023/A:1014995523521. PMID 12005023. Kohfeldt E, Sasaki T, Göhring W, Timpl R (Sep 1998). Nidogen-2: a new basement membrane protein with diverse binding properties. Journal of Molecular Biology. 282 (1): 99-109. doi:10.1006/jmbi.1998.2004. PMID 9733643. Entrez Gene: NID2 nidogen 2 (osteonidogen). Ulazzi L, Sabbioni S, Miotto E, Veronese A, Angusti A, ...

Lymph node stromal cells (LNSC) regulate immunity through constructing lymphocyte niches. LNSC produced Laminin α5 (Lama5) regulates CD4 T cells but the underlying mechanisms of its functions are poorly understood. Here we showed depleting Lama5 in LNSC resulted in decreased Lama5 protein in the LN cortical ridge (CR) and around high endothelial venules (HEV). Lama5 depletion affected LN structure with increased HEV, upregulated chemokines and cell adhesion molecules, and led to greater numbers of Treg in T cell zone. Mouse and human T cell transendothelial migration and T cell entry to LN were suppressed by Lama5 through the receptors a6 integrin and α-dystroglycan. During immune responses and allograft transplantation, depleting Lama5 promoted antigen specific CD4 T cell entry to the CR through HEV, suppressed T cell activation and altered T cell differentiation to suppressive regulatory phenotypes. Enhanced allograft acceptance resulted from depleting Lama5 or blockade of T cell Lama5 ...

Laminin binding integrin receptors are key mediators of epithelial cell migration and tumor metastasis. Recent studies have demonstrated a role for the alpha 6 integrin (ITGA6/CD49f) in maintaining stem cell compartments within normal bone marrow and in residency of tumors metastatic to bone. In this study, we tested a function-blocking antibody specific for ITGA6, called J8H, to determine if pre-existing cancer lesions in bone could be slowed and/or animal survival improved. Human prostate tumors were established by intracardiac injection into male severe combined immunodeficient (SCID) mice and treatment with J8H antibody was initiated after one week. Tumor progression was monitored by micro computed tomography (CT) imaging of skeletal lesions. Animals that received weekly injections of the anti-ITGA6 antibody showed radiographic progression in only 40% of osseous tumors (femur or tibia), compared to control animals, where 80% of the lesions (femur or tibia) showed progression at 5 weeks. ...

DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.

Fibronectin is an extracellular matrix glycoprotein, while lamin is an intermediate filament that specifically provides support to the cell nucleus. Dont confuse lamin with laminin (science hates us clearly); laminin is like fibronectin, an ECM glycoprotein and a major component of the basal lamina of basement membranes.. ...

4 synonyms for lamina: membrane, sheath, sheathing, skin. The lamina densa was formerly known as the basal lamina. The exact composition of the basal lamina varies between different types of cell. [1], The connective tissue of the lamina propria is loose and rich in cells. the layer of connective tissue beneath the epithelial linking of internal organs. integrins. This has led to considerable terminological confusion and, if used, the term basal lamina should be confined to its meaning as lamina densa. También puede contener proteasas y factores de crecimientos como el transformante beta, de fibroplastos, entre otros. A basal layer, or stratum basale, is the deepest of the three layers. [6] It has been suggested that myofibroblasts also reside in the lamina propria of several organs. [1] [2 The term basal lamina is usually used with electron microscopy, while the term basement membrane is usually used with light microscopy. Basal lamina vs. basement membrane [edit , edit source] The term ...

The endothelial cells of blood vessels assemble basement membranes that play a role in vessel formation, maintenance and function, and in the migration of inflammatory cells. However, little is known

Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers ...

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In a recent analysis of M-Files by Nucleus Research it was found that M-Files DMS increased productivity in a business at a lower price than the competitors. M-Files is a document management solution, that does far more than reducing paper in filing cabinets. M-Files is an easy document and enterprise content management system meets unique […]. ...

Dear Readers, Here is Part 2 of the Lesson on New Year, New Blessings. I hope you all enjoy every word, please make sure your volume is turned up when you watch the video, it is simply amazing! Last week we learned about the importance of Blessings and how God our Creator wants to bless…

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

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This AbVideo shows you how to create aliquots of BD Matrigel when only small volumes are used at a time. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media. - BD Matrigel™ Aliquoting - AbVideo™ - Support - Abnova

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J:84903 Bolcato-Bellemin AL, Lefebvre O, Arnold C, Sorokin L, Miner JH, Kedinger M, Simon-Assmann P, Laminin alpha5 chain is required for intestinal smooth muscle development. Dev Biol. 2003 Aug 15;260(2):376-90 ...

PhytoRetinol™ has been confirmed to tighten collagen fibers. The expression of Fibrin4, which is the source of elastin protein is increased, and integrin α1, which binds the basement membrane, the dermis and laminin 5, which is the adhesive of the basemen...

50,000 views This week our little lab blog hit a milestone: 50,000 total views. I know this isnt a lot compared with the big science or writing blogs, but its more than we ever expected! This post is a thank you to everyone who has ever dropped by and especially to the regulars who have…

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Complete information for LAMB3 gene (Protein Coding), Laminin Subunit Beta 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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GOL continues with throwbacks. This one felt appropriate as Olivia has just returned to the lab and we met to plan her project. Missed previous GOLs? No worries, here you go

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