TY - JOUR. T1 - Galectin-3, a laminin binding protein, fails to modulate adhesion of human melanoma cells to laminin. AU - Van Den Brule, F. A.. AU - Buicu, C.. AU - Sobel, M. E.. AU - Liu, Fu-Tong. AU - Castronovo, V.. PY - 1995. Y1 - 1995. N2 - Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and ...

TY - JOUR. T1 - Laminin α subunits and their role in C. elegans development. AU - Huang, Cheng Chen. AU - Hall, David H.. AU - Hedgecock, Edward M.. AU - Kao, Gautam. AU - Karantza, Vassiliki. AU - Vogel, Bruce E.. AU - Hutter, Harald. AU - Chisholm, Andrew D.. AU - Yurchenco, Peter D.. AU - Wadsworth, William G.. PY - 2003/7/1. Y1 - 2003/7/1. N2 - Laminins are heterotrimeric (α/β/γ) glycoproteins that form a major polymer within basement membranes. Different α, β and γ subunits can assemble into various laminin isoforms that have different, but often overlapping, distributions and functions. In this study, we examine the contributions of the laminin α subunits to the development of C. elegans. There are two α, one β and one γ laminin subunit, suggesting two laminin isoforms that differ by their α subunit assemble in C. elegans. We find that near the end of gastrulation and before other basement membrane components are detected, the α subunits are secreted between primary tissue ...

|strong|Mouse anti Human laminin alpha 2 antibody, clone 5H2|/strong| recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, lami…

The basal lamina that ensheaths skeletal muscle fibers traverses the synaptic cleft at the neuromuscular junction. Synaptic and extrasynaptic portions of the basal lamina contain different laminin beta chains: beta 2 (or s) at synapses and beta 1 (or B1) extrasynaptically. Laminin beta 2 is also confined to synapselike patches on myotube surfaces in vitro, whereas beta 1 is present throughout the extracellular matrix. This differential localization of laminin beta chains was analyzed by expression of chimeric beta 1-beta 2 molecules in cultured mouse myotubes. A 16-amino acid carboxyl-terminal sequence in beta 2 was necessary for synaptic localization, and an amino-terminal domain in beta 1 promoted association with extracellular fibrils. The synaptic targeting sequence of beta 2 contains a site previously shown to be adhesive for motor neurons. ...

Laminin alpha2 deficiency presents at birth with muscle weakness, hypotonia, and usually asymptomatic white matter signal on MRI. Few patients with laminin alpha2 deficiency have been described with seizures and structural brain abnormalities. The re

Transfection of a wild-type laminin γ2 cDNA in LSV5 keratinocytes rescues expression of functional laminin 5 molecules (Gagnoux-Palacios et al. 1996). Similar to laminin 5 produced by wild-type keratinocytes, the recombinant laminin 5 secreted by the reverted LSV5 cells undergoes extracellular maturation, including proteolytic excision of the NH2-terminal domains of the γ2 short arm. In this study, we examined the functional role of the laminin γ2 short arm by complementing the genetic defect of LSV5 cells by transfer of mutant γ2 cDNAs. Expression of the mutant cDNAs demonstrated that all the different recombinant γ2 chains associate with the endogenous laminin α3 and β3 polypeptides to assemble into α3β3γ2 heterotrimers, and that the NH2-terminal domains of the γ2 chain are required for deposition and incorporation of laminin 5 in the ECM.. Our results, for the first time, define a physiologic function for the short arm of the γ2 chain. We demonstrate that mutant laminin 5 ...

To study genes that may be crucial for the male germ cell development of Drosophila we screened a cDNA expression library with a polyclonal antiserum against testis proteins of Drosophila hydei. We identified a cDNA fragment that exhibited a complete sequence similarity with the cDNA of the laminin B2 chain, an important component of the extracellular matrix. Transcripts of laminin B2 were detected in the RNA of male germ cells with the polymerase chain reaction and by in situ hybridization. We studied the reaction of different polyclonal antibodies including those against a Drosophila laminin B2-lac fusion protein, the entire Drosophila laminin complex, or against the mouse laminin complex and against laminin A and B1 chains with specific structures in developing male germ cells of Drosophila. Antigenic sites against laminin B2 were found in the lampbrush loops in primary spermatocyte nuclei, in nuclei of spermatids, and in heads of spermatozoa. The axonemes of elongating spermatids react with ...

Precise contact between epithelial cells and their underlying basement membrane is crucial to the maintenance of tissue architecture and function. To understand the role that the laminin receptor dystroglycan (DG) plays in these processes, we assayed cell responses to laminin-111 following conditional ablation of DG gene (Dag1) expression in cultured mammary epithelial cells. Strikingly, DG loss disrupted laminin-111-induced polarity and beta-casein production, and abolished laminin assembly at the step of laminin binding to the cell surface. Dystroglycan re-expression restored these deficiencies. Investigations of the mechanism revealed that DG cytoplasmic sequences were not necessary for laminin assembly and signaling, and only when the entire mucin domain of extracellular DG was deleted did laminin assembly not occur. These results demonstrate that DG is essential as a laminin-111 co-receptor in mammary epithelial cells that functions by mediating laminin anchoring to the cell surface, a process that

TY - JOUR. T1 - Mild clinical phenotype in a 12-year-old boy with partial merosin deficiency and central and peripheral nervous system abnormalities. AU - Mora, Marina. AU - Moroni, Isabella. AU - Uziel, Graziella. AU - Di Blasi, Claudia. AU - Barresi, Rita. AU - Farina, Laura. AU - Morandi, Lucia. PY - 1996/10. Y1 - 1996/10. N2 - We found partial merosin deficiency in a boy presenting at 12 yr with marked limb weakness and a waddling gait. Magnetic resonance imaging (MRI) showed the characteristic white matter abnormalities of merosin-negative congenital muscular dystrophy. There were also peripheral demyelinating polyneuropathy and evoked potential abnormalities. Unlike classic merosin-negative congenital muscular dystrophy, however, our patient was less hypotonic and weak and was able to achieve independent walking. Both by immunohistochemistry and Western blot merosin was shown to be moderately reduced. By immunostaining the α1 laminin chain was overexpressed and the β1 laminin chain was ...

The ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...

TY - JOUR. T1 - Structural analysis of a human glial variant laminin. AU - LeMosy, Ellen. AU - Lightner, Virginia A.. AU - Erickson, Harold P.. PY - 1996/8/25. Y1 - 1996/8/25. N2 - Astrocytes secrete laminin-like molecules in culture and may represent a major source of laminin in the developing central nervous system, yet these laminins have not been extensively characterized. We previously reported the presence of an astrocyte-derived variant laminin in media conditioned by human U251 MG astrocytoma cells. This laminin was partially purified in a highly anionic Mono Q fraction with strong adhesion activity for fibroblasts and glial cells (Aukhil et al. (1990) Matrix 10: 98-111). We now show that glial laminin could be dissociated from an anionic species, perhaps an ~400- kDa keratan sulfate proteoglycan present in the preparation, by a second round of Mono Q anion exchange chromatography in the presence of 6 M urea. Cell adhesion activity remained tightly associated with laminin-containing ...

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013 ...

Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.

Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.

BACKGROUND: LDL oxidation in the vascular wall is associated with aldehyde modification of surrounding extracellular matrix proteins that may target autoimmune responses against vascular tissues. Here we investigated the possible influence of immunity against a malondialdehyde (MDA)-modified form of the basement membrane protein laminin on atherosclerosis. METHODS AND RESULTS: IgM and IgG autoantibodies were present in human plasma and a prospective clinical study demonstrated that individuals who later suffered from acute cardiovascular events had lower levels of MDA-laminin antibodies compared to those in the control group. Immunohistochemical analysis of atherosclerotic plaques from Apoe(-/-) mice demonstrated co-localization between laminin and MDA epitopes, however MDA-laminin IgG was absent in mouse plasma. To determine the effect of MDA-laminin immunity, Apoe(-/-) mice were immunized with MDA-laminin. Analysis of circulating leukocytes at 12 weeks demonstrated increased T-cell activation, ...

Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,

Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,

Laminins are continuously expressed during all developing stages of cartilage and cartilage-like tissues and show a region-specific expression pattern. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone around the chondrocytes (Kvist, 2008). Of the laminin subunits surveyed, α1, α5, β1, and γ1 have shown the highest staining intensity (Kvist, 2008). Lee and colleagues found that laminin chains (α1, α2, β1, β2, and γ1), produced by chicken embryo sternal chondrocytes, exhibited an increased expression in aggregated cells during the maturation stage (Lee, 1997). In newborn mouse knee cartilage, laminin 111 showed staining in the extracellular matrix, while laminin α5 (511 and 521) stained weakly at this point (Kvist, 2008). The laminin β1 chain is expressed from gestational weeks (gw) 10 ...

After the game, we had FCA, its the bomb, and Mr. Sheek talked about Laminin. Laminin by definition is the basement membrane, which is a protein network foundation for most cells and organs. Now youre wondering why in the world you should keep reading this, and why i am telling you this. Well Im here to tell you that Laminin is what our bodies is made up of. Scripture says that God is in us. And people wonder how in the world can God be In us. Well Laminin is proof. Laminin is, well let me show you what Laminin is. ...

Corning™ rLaminin-521 (Human) is an animal-component-free recombinant heterotrimer composed of a5, B2, and y1 chains expressed in a mammalian cell culture system. The Corning rLaminin-521 (Human) surface supports such key benefits as ROCK inhibitor independent, single-cell expansion of PSCs and inhibition of spontaneous differentiation, improving hPSC culture ease and efficiency.. A collaboration focused on bringing together better solutions for stem cell research. Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell ...

Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell processing market, enabling our customers to scale-up effectively and mitigate risk.. Now backed by Cornings large network, worldwide distribution channels, and cell culture portfolio, researchers globally have better access and availability to rLaminin-521 (Human) than ever before. rLaminin-521 (Human) joins the Corning ECM mimetic and advanced surfaces family: animal component-free surfaces suited for stem and progenitor cell expansion under cGMP processing environments. ...

Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...

Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...

Genetic variation in a member of the laminin gene family affects variation in body composition in Drosophila and humans. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The ...

Monoclonal clone# G2 antibody for LAMA1; LAMA2; LAMB1; LAMB2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Feline. LAMA1; LAMA2; LAMB1; LAMB2 information: Molecular Weight: 196474 MW; Subcellular Localization: Sec

The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.

In this work we analyzed data obtained from case-history of 36 patients operated in Hospital for tumors, Zagreb in period 2006 till 2007 year. Ductal breast cancer in situ (DCIS) were diagnosed in 24 patients while in 12 patients diagnosis was ductal micro invasive breast cancer. Aim of thesis was to define expression of polyclonal and monoclonal anti-laminin antibodies on basement membrane of ducts in breast cancer using immunohistochemical method. We investigated whether loss of laminin in basement membrane can be a predictive marker for micro invasiveness. We also investigated association between prognostic factors as age of patients, tumor differentiation stage, steroid hormone receptors and HER2 status with anti-laminin expression and micro invasion appearance. Average age of patients was 56.5 years. The results showed that polyclonal anti-laminin is an important predictive factor of micro invasion appearance in DCIS (p=0,00026) comparing to monoclonal anti-laminin (p=0,23765) (p,0,05 ). ...

Schuger L, Yurchenco P, Relan NK, Yang Y. Laminin fragment E4 inhibition studies: basement membrane assembly and embryonic lung epithelial cell polarization requires laminin polymerization. Int J Dev Biol. 1998 Mar; 42(2):217-20 ...

Laminins are glycoproteins that are major constituents of the basement membrane of cells. Laminins are trimeric molecules; laminin-1 is an alpha1 beta1 gamma1 trimer. It has been suggested that the domains I and II from laminin A, B1 and B2 may come together to form a triple helical coiled-coil structure [(PUBMED:3182802)]. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components.. ...

Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from α, β and γ chain subunits, secreted and incorporated into cell-associated extracellular matrices. The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. Through these interactions, laminins critically contribute to cell differentiation, shape and movement, maintenance of tissue phenotypes, and promotion of tissue survival [1,2]. The different laminin chains share a 600-residue domain I/II which oligomerizes into a rod-like coiled-coil structure forming the long arm of laminins. The N-terminal short arms consist of rod-like elements (domain III and V) formed by tandem arrays of laminin-type EGF modules (see ,PDOC00961,) and several globular domains: domains IV and domain VI (laminin N-terminal) (see ,PDOC51117,). All α ...

Laminin, an extracellular matrix protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.[3] ...

We use the same antibody at 1:20 with proteinase k from DAKO for 10min at RT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Coolen, Neeltje Sent: Thursday, April 07, 2005 3:37 AM To: [email protected] Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje ...

Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. The mitogenic activity of laminin is restricted to a fragment which consists of about 25 repeating domains with partial homology to EGF and comprises the rod-like inner regions of the three short arms of the four armed molecule. The mitogenic activity does not correlate with promotion of cell attachment and neurite outgrowth for which major functional sites have been found in other regions of the laminin molecule. It is suggested that EGF-like domains in laminin, in other ECM proteins and in the extracellular portions of some membrane proteins are signals for cellular growth and differentiation. Because they are integral parts of large molecules and often of supramolecular ...

Laminin-5 is a trimer of laminin α3, β3 and γ2 chains that is found in the intestinal basement membrane. Deposition of the laminin γ2 chain at the basement membrane is of great interest because it undergoes a developmental shift in its cellular expression. Here we study the regulatory elements that control basal and cytokine-activated transcriptional expression of the LAMC2 gene, which encodes the laminin γ2 chain. By using transient transfection experiments we demonstrated the presence of constitutive and cytokine-responsive cis-elements. Comparison of the transcriptional activity of the LAMC2 promoter in the epithelial HT29mtx cells with that in small-intestinal fibroblastic cells (C20 cells) led us to conclude that two regions with constitutive epithelium-specific activity are present between positions -1.2 and -0.12 kb. This was further validated by transfections of primary foetal intestinal endoderm and mesenchyme. A 2.5 kb portion of the LAMC2 5ʹ flanking region was equally ...

Description: A polyclonal antibody for detection of Laminin Alpha-3 from Human, Mouse, Rat. This Laminin Alpha-3 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Laminin ?-3 at AA rangle: 2540- ...

Laminin beta 1兔多克隆抗体(ab69633)可与人样本反应并经WB, ELISA, IHC, ICC/IF实验严格验证。所有产品均提供质保服务，中国75%以上现货。

Laminin is a major component of the extracellular matrix (ECM) and arose early in animal evolution. Due to its role in cell adhesion and migration, laminin is central to development and disease. Laminin is a heterotrimer with a total mass of up to 800 kD. Its three subunits (α, β, and γ) assemble into a distinctive cross-shaped structure, copies of which further self-assemble to form a network. Higher organisms contain a number of isotypes of each subunit, which produce, combinatorially, a rich family of related proteins with certain structural and functional distinctions. Many fundamental aspects of laminin structure are understood from rotary shadowing electron microscopy, recognizable amino acid sequence motifs (i.e., coiled-coil heptad repeats), and X-ray crystallography of laminin fragments. However, other conserved portions of laminin, namely the L4, LF, and Lβ knob domains, are still poorly understood, both structurally and functionally. The L4 and LF domains are ...

Use Corning Laminin surfaces as a thin coating on tissue culture surfaces or as a soluble additive to culture medium to promote cell adhesion, migration, chemotaxis, growth, and differentiation. Laminin/Entactin Complex High Concentration (HC) is a special formulation that Corning has developed to enhance three-dimensional (3D) cell culture applications. Culturing cells in or on gels such as Laminin/Entactin Complex enable the study of specific mechanisms that dictate cell differentiation and functionality.. Applications include:. ...

Laminin大鼠单克隆抗体[MEC5](ab2466)可与小鼠, 大鼠, 人样本反应并经IHC实验严格验证，Abcam Laminin抗体被2篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务，中国75%以上现货。

Am. J. Respir. Cell Mol. BioI. 1996. Here, the authors studied the expression of different laminin chains and their integrin receptors in fetal and adult lung by immunohistochemistry. Special attention was focused on the changes in the expression of these proteins during the development from the pseudoglandular (PG) and canalicular stages to adult lung and on the possible implications of the changes for the normal lung development. The most significant changes in the expression pattern were found during the development from the PO stage to the canalicular stage. Basement membranes (BM) of both the epithelial buds and the becoming bronchi showed reactivity for laminin α1, a3, and B3 chains at all stages. The a2 chain was expressed only in the epithelial buds at the PO stage, and could not be found in any epithelial structures at the canalicular stage. Similarly, at the PG stage the B2 chain was expressed in BMs of both epithelial buds and bronchi but disappeared from the bronchial BM before the ...

Recent studies, on cells cultured in 3D collagen gels, have shown that, beside from their well known biochemical role, fibronectin (FN) and laminin (LM) affect cell functions via a modification of mec

Laminin gamma 1 antibody, Internal (laminin subunit gamma 1) for WB. Anti-Laminin gamma 1 pAb (GTX44865) is tested in Human samples. 100% Ab-Assurance.

Laminin alpha 4 antibody [C3], C-term (laminin, alpha 4) for IHC-P. Anti-Laminin alpha 4 pAb (GTX106134) is tested in Mouse samples. 100% Ab-Assurance.

Laminin SIKVAV peptide induction of monocyte/macrophage prostaglandin E2 and matrix metalloproteinases.: The laminin-derived synthetic peptide containing the SI

Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical study, Sara Amini, Mohammad Reza Nikravesh, Mehdi Jal

Humans and mice with deficiency of the alpha2 subunit of the basement membrane protein laminin-2/merosin suffer from merosin-deficient congenital muscular dystrophy (MCMD). We have expressed a human laminin alpha2 chain transgene under the regulation of a muscle-specific creatine kinase promoter in …

in Journal of the National Cancer Institute (1992), 84(15), 1161-9. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin ... [more ▼]. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin-binding proteins, including the 67-kd laminin receptor (67 LR) and a 31-kd human laminin-binding protein (HLBP31) homologous to the 31-kd human IgE-binding protein/galactoside-binding lectin. PURPOSE: To investigate whether various laminin-binding proteins are differentially expressed in human colon carcinoma, we studied messenger RNA (mRNA) levels of the 67 LR and HLBP31 in matched tumor and adjacent normal mucosa samples from a series of 21 patients. METHODS: Total cellular RNA from tumor and normal mucosa was isolated ...

Schwann cell basement membranes do not appear to form in developing tissues until the stage of neuronal ensheathment, and it has been postulated that axonal contact triggers their assembly, in turn affecting cell polarization, ensheathment and myelination ( Clark and Bunge, 1989; Jaakkola et al., 1993). The data presented in this study suggest that Schwann cell basement membrane assembly is a process that first requires the development of cell surface competency for laminin binding, followed by the binding and assembly of laminin. We consider this ECM as a `nascent basement membrane (i.e. a laminin-based ECM with classical ultrastructural morphology of a basement membrane, but still lacking in type IV collagen). The nascent basement membrane is likely to be no more than one laminin molecular layer thick. Upon exposure of the cells to type IV collagen, this component is incorporated into the laminin matrix. In Schwann cells, the laminin binding/assembly step depends in part upon an interaction ...

Korpos et al. (1) claim that limited information exists on the nature of the ECM [extracellular matrix] of the pancreas and, in particular, on the composition of peri-islet capsule, and that they provide the the first comprehensive analysis of the [ECM] composition of peri-islet capsules.. We dispute the strength of these claims. In mice we originally reported in 2008 that a basement membrane (BM) exists around islets, and eight unique components were identified (type IV collagens α1, α2, laminin α2, β1, γ1, nidogen 1, nidogen 2, perlecan) as well as two composite components (laminin Engelbreth-Holm-Swarm, collagen IV) (2). It was also shown that type IV collagens α3, α4, α5, α6 were not present (2). Korpos et al. (1) were able to find a further three unique components (laminin α4, β2, agrin) and one composite component (laminin 322).. In 2008 in humans (3,4), at least four unique components (laminin α1, α5, β1, γ1) were identified and in their Supplementary Figure 1 staining ...

TY - JOUR. T1 - Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups. AU - Kostrominova, Tatiana. AU - Tanzer, M. L.. PY - 1995. Y1 - 1995. N2 - Laminin glycosyl groups are necessary for the spreading of murine melanoma cells which become attached to this glycoprotein. Laminin has been implicated in myogenesis but the potential role of its glycosyl groups in this process has not been examined. In this study we report the effects of the carbohydrate moieties of laminin on myoblast adhesion, spreading, and differentiation. Unglycosylated laminin from tunicamycin-treated cultures of a mouse cell line, M1536 B3, was used in the experiments. Glycosylated laminin from a murine tumor and from cultures of M1563 B3 cells served as controls. Cell binding experiments with C2C12 mouse myoblasts showed that the cells preferred a laminin-coated surface, compared to the uncoated plastic surface (nontissue culture wells). Myoblasts did not distinguish ...

The disulfide bonding patterns in the N-terminal (LN) domains of the basement membrane protein laminin beta 1 have not been investigated so far. We report an in-depth mass spectrometric analysis using offline nano-high-performance liquid chromatography/matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (nano-HPLC/MALDI-TOF/TOF-MS) for determining the disulfide bond patterns in the LN-domain of recombinant mouse laminin beta 1 chain for the first time. Mass spectra were recorded and the putatively disulfide-linked peptides were subjected to LIFT-TOF/TOF-MS to confirm the disulfide bond. Screening the fragment ion mass spectra of disulfide-linked peptides for characteristic 66-amu patterns (34 u +32 u), arising from symmetric and asymmetric cleavage of disulfide bonds, facilitated their identification. Using various enzymes for proteolytic digestion of a recombinant laminin beta 1 chain N-terminal protein fragment, a linear bonding pattern of the eight ...

The human non-integrin laminin receptor (LamR) is a multifunctional protein which is localised to a number of sub-cellular locations. LamR is a component of the ribosome and has a number of intracellular functions; it also acts as an extracellular receptor for laminin, growth factors, pathogenic microorganisms, prion proteins, toxins and the anticarcinogen epigallocatechin gallate (ECGC). Although LamR is present in most cellular compartments, its overexpression in many types of cancer cells suggests a vital role for LamR in tumor-cell migration and invasion. There are two isoforms of laminin receptor: the monomeric 37-kDa laminin receptor precursor (37LRP) and the mature 67-kDa laminin receptor (67LR). Although the precise molecular nature of 67LR is unclear, accumulating evidence strongly suggest that 37LRP can undergo homo- and/or hetero-dimerization with Galectin-3 (Gal-3) to form mature 67LR. A recent study demonstrated that both homo- and heterodimer LamR forms are present on the cell ...

Laminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell shape change and are organized into an epithelium. Laminin is present on the basal surfaces of ectodermal cells and is absent or reduced on migrating primary mesenchyme cells. Microinjection of a monoclonal antibody directed against laminin induces a morphological change in cell shape and a deformation of the embryonic epithelium. Investigation of selected stages of live embryos suggests that the distribution of laminin may be heterogeneous within the basal lamina during early development. The results implicate laminin as a mediator of cell shape change during early morphogenesis.. ...

Institutions: 1) University of Nottingham, 2) University of Vienna. The myodystrophy mouse is a spontaneous, autosomal recessive model of muscular dystrophy. We previously identified the molecular defect underlying the myodystrophy (Largemyd) mouse as a deletion in the Large gene, encoding a glycosyltransferase. This mutant is currently the only animal model for studying the role of glycosylation in the group of human muscle disorders due to mutated glycosyltransferases.Dystroglycan, an extensively modified glycoprotein, is a central component of the dystrophin-associated glycoprotein complex; this plays an important role in maintaining muscle membrane integrity. Furthermore, glycosylation of ?lpha?dystroglycan is necessary for its interaction with the extracellular matrix protein laminin. Western analyses of Largemyd proteins reveal hypo-glycosylation of alpha-dystroglycan and loss of laminin binding. We are currently using the glycan-binding specificities of lectins to investigate the overall ...

in Invasion & Metastasis (1993), 13(1), 1-30. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several ...

HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show that competency for differentiation is due at least in part to the modulation of extracellular matrix components. In particular we have studied laminin and tenascin. EGF alters endogenous laminin assembly. In addition, promotion of competency can be partially mimicked by plating HC11 cells on the E8 laminin fragment, which is able to induce lactogenic responsiveness in cells grown in the absence of EGF or bFGF. The production and assembly of tenascin is also dependent upon the growth conditions of the HC11 cells. EGF- or bFGF-grown competent cells produce tenascin but do not assemble it at the extracellular matrix as efficiently as Ins- or PDGF-grown, non-competent cells. This ...

Macrophages require activation with either PMA (Mercurio, A. M., and L. M. Shaw. 1988. J. Cell Biol. 107:1873-1880) or interferon-gamma (Shaw, L. M., and A. M. Mercurio. 1989. J. Exp. Med. 169:303-308) to adhere to a laminin substratum. In the present study, we identified an integrin laminin receptor on macrophages and characterized cellular changes that occur in response to PMA activation that facilitate laminin adhesion. A monoclonal antibody (GoH3) that recognizes the integrin alpha 6 subunit (Sonnenberg, A., H. Janssen, F. Hogervorst, J. Calafat, and J. Hilgers. 1987. J. Biol. Chem. 262:10376-10383) specifically inhibited adhesion to laminin-coated surfaces. This antibody precipitated an alpha 6 beta 1 heterodimer (Mr 130/110 kD) from 125I surface-labeled macrophages. The amount of radiolabeled receptor on the cell surface did not increase after PMA activation. Thus, the induction of laminin adhesion cannot be attributed to de novo or increased surface expression of alpha 6 beta 1. By ...

TY - JOUR. T1 - Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle. AU - Gulati, A. K.. AU - Reddi, A Hari. AU - Zalewski, A. A.. PY - 1984. Y1 - 1984. N2 - Extracellular matrix is known to play an important role during development and maintenance of various tissues. In the present study, changes in two extracellular matrix glycoproteins, fibronec ?ptin and laminin, were investigated in skeletal muscle undergoing immune rejection. Purified antibodies against fibronectin and laminin were used to analyze the matrix by indirect immunofluorescence at various intervals after transplantation of extensor digitorum muscle in rats. Fibronectin and laminin were localized in the pericellular basement membrane zone of the normal myofibers; however, the cytoplasm was devoid of both glycoproteins. Transplanted muscle grafts underwent a process of degeneration and then an initial regeneration during the first 7 days. This regeneration effort ceased ...

Looking for online definition of laminin, alpha 4 in the Medical Dictionary? laminin, alpha 4 explanation free. What is laminin, alpha 4? Meaning of laminin, alpha 4 medical term. What does laminin, alpha 4 mean?

In humans, mutations in the lama2 gene, which encodes laminin a2, cause merosin-deficient congenital muscular dystrophy 1A MDC1A , one subset of a broad

The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basement membrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton. We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted ...

Endothelial basement membranes constitute barriers to extravasating leukocytes during inflammation, a process where laminin isoforms define sites of leukocyte exit; however, how this occurs is poorly understood. In addition to a direct effect on leukocyte transmigration, we show that laminin 511 affects endothelial barrier function by stabilizing VE-cadherin at junctions and downregulating expression of CD99L2, correlating with reduced neutrophil extravasation. Binding of endothelial cells to laminin 511, but not laminin 411 or non-endothelial laminin 111, enhanced transendothelial cell electrical resistance (TEER) and inhibited neutrophil transmigration. Data suggest that endothelial adhesion to laminin 511 via beta1 and beta3 integrins mediates RhoA-induced VE-cadherin localization to cell-cell borders, and while CD99L2 downregulation requires integrin beta1, it is RhoA-independent. Our data demonstrate that molecular information provided by basement membrane laminin 511 affects leukocyte ...

Neuromuscular junction (NMJ) assembly is characterized by the clustering and neuronal alignment of acetylcholine receptors (AChRs). In this study we have addressed post-synaptic contributions to assembly that may arise from the NMJ basement membrane with cultured myotubes. We show that the cell surface-binding LG domains of non-neural (muscle) agrin and perlecan promote AChR clustering in the presence of laminin-2. This type of AChR clustering occurs with a several hour lag, requires muscle-specific kinase (MuSK), and is accompanied by tyrosine phosphorylation of MuSK and betaAChR. It also requires conjugation of the agrin or perlecan to laminin together with laminin polymerization. Furthermore, AChR clustering can be mimicked with antibody binding to non-neural agrin, supporting a mechanism of ligand aggregation. Neural agrin, in addition to its unique ability to cluster AChRs through its B/z sequence insert, also exhibits laminin-dependent AChR clustering, the latter enhancing and stabilizing ...

Objective:To investigate the effects of puerarin on renal structure?function and expressions of MMP-3 and TIMP-1 in the kidney of diabetic rats.Methods:Uninephrectomized STZ-induced male wister rats were used.Puerarin was delivered daily by the intraperitoneal injection from the third day of induction to diabetes for 16 weeks.Using in situ hybridization and immunohistochemistry to detect TIMP-1mRNA expression and MMP-3?TIMP-1?collagen Ⅳ and Laminin expressions in diabetic kidneys with image analysis system;Flow cytometry was used to detect the expressions of MMP-3?TIMP-1.Results:BUN?Ccr and Upro in diabetic rats were significant higher than that in control group.Compared with those in kidneys of control group,diabetic kidneys showed increased expressions of both MMP-3?TIMP-1 proteins and excessive deposition of glomeruli extracellular matrix(P0.05).The blood glucose and the level of expressions of MMP-3?TIMP-1?collagen Ⅳ and Laminin were reduced by puerarin treatment,renal function was improved

Laminin Receptor antibody LS-C197601 is an unconjugated mouse monoclonal antibody to human Laminin Receptor (RPSA). Validated for ELISA and WB.

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In previous studies, researchers at the Cells-in-Motion Cluster of Excellence had discovered that specific proteins in the basement membrane - so-called laminins - influence the migration of the immune cells. For example, they determine the place at which immune cells cross the basement membrane and enter into the tissue. Researchers have now discovered that these components of the basement membrane also affect the inner endothelial cell layer.. A certain laminin - laminin 511 - influences how tightly endothelial cells are connected to each other, explains biochemist Prof. Lydia Sorokin. This is because laminin affects specific molecules that interconnect endothelial cells. For one thing, laminin stabilizes the VE-cadherin molecule at contact sites between adjacent endothelial cells, forming a sort of Velcro fastener between them. In addition, laminin 511 reduces the quantity of a molecule known as CD99L2.. This is found on both the endothelial cells and on the immune cells and permits the ...

Mokkapati, Sharada (2007) Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin. [Thesis Abstract]. ...

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture pla

The basement membrane (BM) is a specialized form of extracellular matrix (ECM) underlying epithelia and endothelia and surrounding many types of mesenchymal cells. Nidogen, along with collagen IV and laminin, is a major component of BMs. Although certain ECM proteins such as laminin or reelin influence neuronal function via interactions with cell-surface receptors such as integrins, behavioral neurological impairments due to deficits of BM components have been recognized only recently. Here, alterations in neuronal network function underlying these behavioral changes are revealed. Using nidogen-1 knockout mice, with or without additional heterozygous nidogen-2 knockout (NID1(-/-)/NID2(+/+) or NID1(-/-)/NID2(+/-)), we demonstrate that nidogen is essential for normal neuronal network excitability and plasticity. In nidogen-1 knockouts, seizurelike behavior occurs, and epileptiform spiking was seen in hippocampal in vivo EEG recordings. In vitro, hippocampal field potential recordings revealed that ...

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Stem cell culture is accompanied by extra challenges, and it is critical to maintain genetic stability, ensure stemness, and prevent spontaneous differentiation. Cellartis media and supplements are optimized for derivation, maintenance, and expansion of pluripotent cells.. The Cellartis DEF-CS system is a complete culture system for robust expansion of human iPS cells in a feeder-free and defined environment. A xeno-free version is also available.. iMatrix-511 is a xeno-free recombinant laminin fragment for ES or iPS cell culture substrate; iMatrix-511 provides greater adhesive properties than full-length laminin, vitronectin, or reagents containing an undefined mixture of basement membrane.. ...

Integrin alpha6beta1: A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.

Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…

Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…

A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...

A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...

Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Additional information: Clone REA276 displays negligible binding to Fc receptors.

Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease.Additional information: Clone REA276 displays negligible binding to Fc receptors.

Background and Objective: It is obviously approved that Fibronectin and Laminin α-5 (LAMA5) expression will change, in some organs of fetuses that their mothers were smoking during pregnancy period. For example, the expression of mentioned proteins in newborns decreases in parenchyma of their lungs. In present study, we evaluated the effects ...

Brain-specific homing and direct interactions with the neural substance are prominent hypotheses for brain metastasis formation and a modern manifestation of Pagets seed and soil concept. However, there is little direct evidence for this neurotropic growth in vivo. In contrast, many experimental studies have anecdotally noted the propensity of metastatic cells to grow along the exterior of pre-existing vessels of the CNS, a process termed vascular cooption. These observations suggest the soil for malignant cells in the CNS may well be vascular, rather than neuronal. We used in vivo experimental models of brain metastasis and analysis of human clinical specimens to test this hypothesis. Indeed, over 95% of early micrometastases examined demonstrated vascular cooption with little evidence for isolated neurotropic growth. This vessel interaction was adhesive in nature implicating the vascular basement membrane (VBM) as the active substrate for tumor cell growth in the brain. Accordingly, VBM

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Science Meets God Many of you are aware of my background in Exercise and Nutritional Sciences, here is where Science meets God. Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. The laminins are an important and biologically active…

Business Owner Linda Morris published a press release titled: The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..

Business Owner Linda Morris published a press release titled: Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?

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