0043]In one exemplary embodiment, a transformed bacterial host cell with a mutant ferulic acid esterase gene is constructed. A mutant ferulic acid esterase gene is inserted into a pG+host5 vector. The mutant ferulic acid esterase gene is then transferred into bacterial host cells using electroporation. The mutant ferulic acid esterase then becomes part of the bacterial host cells genome by recombination. The bacterial host cells can be Lactobacillus buchneri cells. In some embodiments, the Lactobacillus buchneri cells will be Lactobacillus buchneri strain PTA-6138 cells. The mutant ferulic acid esterase gene can be a knock out. New strains of Lactobacillus buchneri with mutant, including knocked-out ferulic acid esterase, can be used as negative controls. For example, a transformed Lactobacillus buchneri strain can be used in an assay for studying the impact of ferulic acid esterase activity. One example of such an assay would be inoculating whole plant material, such as whole plant silage, with ...
A feeding trial was carried out effect of supplemental Lactobacillus on productivity, egg quality and intestinal microflora in 320 21 weeks - old laying hens for 12 week. Supplemented Lactobacillus strains were Lactobacillus amylovorus LLA7(LA), Lactobacillus crispatus LLA9(LC) and Lactobacillus vaginalis LLA11(LV). Three strains mixed to basal diet which containing 2,800㎉/kg ME, 16% CP with none, LA, LC, LV, LA+LC, LA+LV, LC+LV and LC+LC+LV. Supplemental level was 10(sup)7 cfu/g diet. Egg production was tended to increase with adding Lactobacilus, but not difference significantly. Average egg weight was heavier in adding Lactobacillrs compared to the none, and heaviest in LA+LV, LC+LV(P〈0.05). In periodic observation, the gap of egg weight with adding Lactobacillus or not was severe persisting laying periods. The diet containg MC or LV was better than LA, which means the difference by Lactobacillus strains for egg weight. Daily egg mass also increased in adding Lactobacillus about 1.1 to 2.3 g
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Cervical cancer is a human papilloma virus (HPV)-related cancer, but most HPV infections are transient or intermittent and resolve spontaneously. Thus, other factors, such as cervical microflora, which are dominated by lactobacilli, must be involved in invasive cervical carcinoma development after HPV infection. Previous studies have demonstrated that lactobacilli have antitumour effects, and it is possible that vaginal lactobacilli prevent cervical cancer. Here we examined the proliferative and apoptotic responses of normal and tumour cervical cells to common vaginal lactobacilli components by investigating human normal fibroblast-like cervical (normal cervical) and HeLa (cervical tumour) cell responses to Lactobacillus gasseri and Lactobacillus crispatus. The effects of different lactobacilli components, such as culture supernatants, cytoplasmic extracts, cell-wall extracts and live cells, were determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, trypan blue staining
A Gram-stain-positive bacterial strain, 395-6.2T, was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, in silico DNA-DNA hybridisation (isDDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2T was phylogenetically related to Lactobacillus farciminis , Lactobacillus formosensis , Lactobacillus futsaii , Lactobacillus crustorum , Lactobacillus nuruki , Lactobacillus heilongjiangensis , Lactobacillus musae , Lactobacillus nantensis , Lactobacillus mindensis , Lactobacillus kimchiensis , Lactobacillus zhachilii, Lactobacillus alimentarius , Lactobacillus bobalius , Lactobacillus kimchii and Lactobacillus paralimentarius . Strain 395-6.2T
Twenty morphologically different strains were chosen from French wheat sourdough isolates. Cells were Gram-positive, non-spore-forming, non-motile rods. The isolates were identified using amplified-fragment length polymorphism, randomly amplified polymorphic DNA and 16S rRNA gene sequence analysis. All isolates were members of the genus Lactobacillus. They were identified as representing Lactobacillus plantarum, Lactobacillus paralimentarius, Lactobacillus sanfranciscensis, Lactobacillus spicheri and Lactobacillus sakei. However, two isolates (LP38T and LP39) could be clearly discriminated from recognized Lactobacillus species on the basis of genotyping methods. 16S rRNA gene sequence similarity and DNA-DNA relatedness data indicate that the two strains belong to a novel Lactobacillus species, for which the name Lactobacillus hammesii is proposed. The type strain is LP38T (=DSM 16381T=CIP 108387T=TMW 1.1236T).
Lactobacillus jensenii ATCC ® 25258D™ Designation: Genomic DNA from Lactobacillus jensenii strain 62G TypeStrain=True Application:
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TY - JOUR. T1 - The effects of Lactobacillus gasseri OLL2809 intake on oral immune function in college rugby athletes. AU - Hanaoka, Yukichi. AU - Shimizu, Kazuhiro. AU - Washiya, Kosuke. AU - Tamura, Akira. AU - Takemura, Masahiro. AU - Furukawa, Takuo. AU - Miyamoto, Toshikazu. AU - Watanabe, Koichi. AU - Akama, Takao. N1 - Publisher Copyright: © 2015, Japanese Society of Physical Fitness and Sports Medicine. All rights reserved.. PY - 2015. Y1 - 2015. N2 - The purpose of this study was to investigate the effects of Lactobacillus gasseri OLL2809 (MG2809) intake on salivary secretory immunoglobulin A (SIgA) secretion and incidence of upper-respiratory tract infection (URTI) symptoms among college rugby athletes. Sixty-seven subjects were assigned to a MG2809 group (n = 33) or a placebo group (n = 34) using a double-blind procedure. Each subject took MG2809 or placebo tablets for 9 weeks. Resting saliva samples were collected before (0 week) and after 2, 4, 6, 8, and 9 weeks of the MG2809 ...
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The mammalian gut microbiota is composed of autochthonous species that permanently colonize the host intestine, and of allochthonous species that are only transiently able to occupy the intestinal environment. In this thesis research, Lactobacillus ruminis and Lactobacillus rhamnosus GG were investigated as paradigms for each type of microbe-host interaction, with special emphasis on the in vitro characterization of their adaptation factors in the host GIT. L. rhamnosus GG has two pilus operons: spaCBA encoding the well-studied SpaCBA pili and spaFED putatively encoding SpaFED pili. The expression of SpaFED pili in L. rhamnosus GG under laboratory conditions has not thus far been reported. In this study, a nisin-induced expression system was used for the generation of SpaFED or SpaF-deleted pili in Lactococcus lactis NZ9000. The results revealed that SpaFED pili were essential in mediating lactococcal adhesion to intestinal cell lines, to certain extracellular matrix proteins, and to porcine ...
The mammalian gut microbiota is composed of autochthonous species that permanently colonize the host intestine, and of allochthonous species that are only transiently able to occupy the intestinal environment. In this thesis research, Lactobacillus ruminis and Lactobacillus rhamnosus GG were investigated as paradigms for each type of microbe-host interaction, with special emphasis on the in vitro characterization of their adaptation factors in the host GIT. L. rhamnosus GG has two pilus operons: spaCBA encoding the well-studied SpaCBA pili and spaFED putatively encoding SpaFED pili. The expression of SpaFED pili in L. rhamnosus GG under laboratory conditions has not thus far been reported. In this study, a nisin-induced expression system was used for the generation of SpaFED or SpaF-deleted pili in Lactococcus lactis NZ9000. The results revealed that SpaFED pili were essential in mediating lactococcal adhesion to intestinal cell lines, to certain extracellular matrix proteins, and to porcine ...
Twelve strains isolated from the posterior fornix fluid of the human vagina were identified as Lactobacillus johnsonii, Lactobacillus acidophilus, Lactobacillus gallinarum and Lactobacillus crispatus based on numerical analyses of total soluble cell protein profiles and randomly amplified polymorphic DNA (RAPD)-PCR banding patterns. Five strains grouped with the type strains of Lactobacillus gasseri (DSM 20077T) and Lactobacillus jensenii (DSM 20557T) at r | or = 0.83 in one protein profile cluster, well separated from the other species included in this study. However, numerical analysis of the RAPD-PCR banding patterns of representative strains selected from the L. gasseri-L. jensenii protein cluster clearly indicated that they belong to two different species. Four strains (TV 1010, TG 1013, TV 1018T and TV 1045) grouped into another well separated protein profile cluster at r | or = 0.87. Strains selected from this cluster displayed very similar RAPD-PCR banding patterns and clustered at R2 | or = 0
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Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus. Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in α-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known glucosyltransferase (gtf) genes of lactic acid bacteria strains allowed cloning of fragments of 10 putative gtf genes from eight different glucan producing Lactobacillus strains (five Lactobacillus reuteri strains, one Lactobacillus fermentum strain, one Lactobacillus sake strain and one Lactobacillus parabuchneri strain). Sequence analysis revealed that these lactobacilli possess a large variation of (putative) gtf genes, similar to what has been observed for Leuconostoc and Streptococcus strains. Homologs of GTFA of Lb. reuteri 121 (synthesizing reuteran, a unique glucan with α(1 → 4) and α-(1 → 6) glycosidic bonds) (Kralj et al., 2002) were found in ...
Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efficient in resolving VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activity against Candida spp., employing in vitro and in vivo assays. BS isolated from vaginal L.
The role of Lactobacillus buchneri in. homo- and heterofermentative lactic acid bacteria after 74. differences between homofermentative and.Lactic acid bacteria can be classified either homo- or heterofermentative according.Used for nearly 5000 years to culture and ferment food, lactic acid.Lactic Acid Bacteria, or Lactobacillus, is the most common type of bacteria found in probiotics.Cultivation of a facultative heterofermentative lactic acid bacteria.Lactic acid bacteria. between homofermentative and heterofermentative.Unformatted text preview: 2 types of lactate producing bacteria, homofermentative and heterofermentative.. If you have an individual access to this publication, or if you have purchased this contribution within the past 24 hours, you can gain access by logging in with your username (your email address) and password ...
Background: Lactobacillus ruminis is a poorly characterized member of the Lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. Its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of complex carbohydrates. Results: In this study, we investigated the ability of nine L. ruminis strains of human and bovine origin to utilize fifty carbohydrates including simple sugars, oligosaccharides, and prebiotic polysaccharides. The growth patterns were compared with metabolic pathways predicted by annotation of a high quality draft genome sequence of ATCC 25644 (human isolate) and the complete genome of ATCC 27782 (bovine isolate). All of the strains tested utilized prebiotics including fructooligosaccharides (FOS), soybean-oligosaccharides (SOS) and 1,3:1,4-b-D-glucooligosaccharides to varying degrees. Six strains isolated from humans utilized FOS-enriched inulin, as well ...
The health benefits attributed to probiotics generate interest in the search of competent strains adapted to several ecological niches, especially those related to traditional beverages and foods of each country. Pineapple tepache, a traditional Mexican fermented beverage, was used for the isolation of lactic acid bacteria with probiotic potential, one of which withstood the in vitro tests. The isolated strain AB-05, which exhibited the tested probiotic functional properties, was designated as Lactobacillus pentosus ABHEAU-05. The sequence was registered in GenBank under access code MK587617. This study is the first report of a lactic acid bacterium with in vitro digestion resistance isolated from pineapple tepache. The survival of L. pentosus ABHEAU-05 in a symbiotic medium was proven using fermented milk enriched with inulin. The in vitro digestion-resistant probiotic activity of lactobacilli was measured through analysis of pH and proteolysis. Results showed that L. pentosus grew properly in ...
A recent study suggests that taking a supplement of the probiotic Lactobacillus paracasei subsp. paracasei LP-33 may significantly improve the quality of life for people suffering from hay fever who are also taking an antihistamine. Participants in the study included 425 people with hay fever who were being treated with the antihistamine loratadine. Over the course of five weeks they were assigned either a placebo or the LP-33 supplement. Severity of hay fever was measured using the Rhinitis Quality of Life (RQLQ) global score.. At the conclusion of the study, the supplement group had significantly lower RQLQ scores when compared with the placebo. While no difference was seen in nasal symptoms, symptoms related to the eyes were significantly improved. Researchers from the French institutions Montpellier I University, University Paris Diderot, Biofortis, and Merck Consumer Health as well as Ruhr-University Bochum in Germany and the Danish institutions Copenhagen University Hospital at Gentofte ...
TY - JOUR. T1 - Differential suppression of heat-killed lactobacilli isolated from kimchi, a Korean traditional food, on airway hyper-responsiveness in mice. AU - Hong, Hye Jin. AU - Kim, Eugene. AU - Cho, Daeho. AU - Kim, Tae Sung. PY - 2010/5. Y1 - 2010/5. N2 - Rationale: Probiotics have been shown to be effective in reducing allergic symptoms. However, there are few studies to evaluate the therapeutic effects of lactobacilli on allergen-induced airway inflammation. Objective: We investigated whether three heat-killed lactobacilli, Lactobacillus plantarum, Lactobacillus curvatus and Lactobacillus sakei subsp. sakei, isolated from kimchi, exerted inhibitory effects on airway hyper-responsiveness in a murine asthma model. Methods: Heat-killed lactic acid bacteria were orally administered into BALB/c mice, followed by challenge with aerosolized ovalbumin, after which allergic symptoms were evaluated. Results: Airway inflammation was suppressed in the L. plantarum- and L. curvatus-treated mice. ...
This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMCs) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMCs from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control. The cytotoxicity was determined by trypan blue exclusion method and IL-1β secretion by ELISA. TNF-α, IL-6, and IL-8 secretions were measured using Bioplex Multiplex Immunoassay. Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMCs than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains
Lactobacillus bulgaricus Lactobacillus bulgaricus is a helpful natural bacterium, and a member of the family microflora, that can fight harmful bacteria that may invade your digestive syst
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Definition of Lactobacillus bulgaricus with photos and pictures, translations, sample usage, and additional links for more information.
A vaginal microbiota dominated by lactobacilli (particularly Lactobacillus crispatus) is associated with vaginal health, whereas a vaginal microbiota not dominated by lactobacilli is considered dysbiotic. Here we investigated whether L. crispatus strains isolated from the vaginal tract of women with Lactobacillus-dominated vaginal microbiota (LVM) are pheno- or genotypically distinct from L. crispatus strains isolated from vaginal samples with dysbiotic vaginal microbiota (DVM). We studied 33 L. crispatus strains (n = 16 from LVM; n = 17 from DVM). Comparison of these two groups of strains showed that, although strain differences existed, both groups degraded various carbohydrates, produced similar amounts of organic acids, inhibited Neisseria gonorrhoeae growth, and did not produce biofilms. Comparative genomics analyses of 28 strains (n = 12 LVM; n = 16 DVM) revealed a novel, 3-fragmented glycosyltransferase gene that was more prevalent among strains isolated from DVM. Most L. crispatus strains showed
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Vaginal probiotics or live biotherapeutic products as defined by the FDA [39] may reduce the risk of HIV transmission by: expressing antiviral factors, restoring the normal microbiota, inhibiting bacterial pathogens and modulating immuno-inflammatory responses without compromising the homeostatic environment of the host. Lactobacilli are commensal Gram-positive bacteria that widely populate the healthy female vaginal mucosa [21, 22, 40, 41]. Several Lactobacillus strains have been implicated by epidemiologic and/or experimental evidence in the maintenance of a homeostatic infection-free microenvironment most notably due to the impact of the bacterias lactic acid and H2O2 production in generating an adverse environment for HIV and other STDs. [21, 40, 42-44]. These properties may contribute to the reduction of viral particles at the site of infection [13, 45]. In contrast, a reduction in the number of Lactobacillus in the vaginal microbiota has been associated with the acquisition of bacterial ...
The research was aimed to determine the present status of probiotics (Lactobacillus spp.) and pathogenic bacteria (Vibrio spp.) of culture pond as well as to determine whether the isolated probiotic Lactobacillus spp. can act as a controlling agent on experimentally pathogenic Vibrio spp. infected Penaeus monodon. In In-vitro test of gills and intestinal tracts, the potential antagonistic activity of Lactobacillus spp. toward Vibrio spp. was gradually observed at 4th hour of probiotic treatment. At 12th hour the viable count of Vibrio spp. was drastically reduced in gill from 2.33×105 to 1.03×103 (CFUg-1) and in intestinal tract 2.35×105 to 6.43×102 (CFUg-1). While, in In-vivo test, in case of muscle, gills and intestinal tract antagonistic activity of Lactobacillus spp. toward Vibrio spp. was noticed after 9 hours, 21 hours and 27 hours respectably of probiotic injected shrimps. However, only the result of in-vitro challenge test revealed that, Lactobacillus spp. significantly reduced the ...
Fura is a millet-based spontaneously fermented dumpling produced and consumed in parts of West Africa, particularly Nigeria, Burkina Faso and Ghana. From eight traditional fura production sites in northern Ghana, 862 lactic acid bacteria were isolated and identified to species level using a combination of genotypic and phenotypic methods including (GTG)5-based PCR fingerprinting and 16S rRNA gene sequencing, multiplex PCR by means of recA gene sequence comparison, conventional morphological characteristics and carbohydrate fermentation profiling. During millet dough fermentation, pH decreased from 5.6-6.4 to 4.1-3.7 and total lactic acid bacteria (LAB) counts increased from 4.4-5.3 to 7.9-9.2 log10 (cfu/g). The initial stages of the fermentation were characterized by co-dominance of homo- and heterofermentative species of Pediococcus acidilactici, Weisella confusa, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus salivarius, and Lactobacillus paraplantarum whereas L. fermentum was ...
The xylose cluster of Lactobacillus pentosus consists of five genes, two of which, xylAB, form an operon and code for the enzymes involved in the catabolism of xylose, while a third encodes a regulatory protein, XylR. By introduction of a multicopy plasmid carrying the xyl operator and by disruption of the chromosomal xylR gene, it was shown that L. pentosus xylR encodes a repressor. Constitutive expression of xylAB in the xylR mutant is repressed by glucose, indicating that glucose repression does not require XylR. The xylR mutant displayed a prolonged lag phase compared to wild-type bacteria when bacteria were shifted from glucose to xylose medium. Differences in the growth rate in xylose medium at different stages of growth are not correlated with differences in levels of xylAB transcription in L. pentosus wild-type or xylR mutant bacteria but are positively correlated in Lactobacillus casei with a plasmid containing xylAB. Glucose repression was further investigated with a ccpA mutant. An ...
This study aimed at evaluating non-starter lactobacilli (NSLAB) isolated from cheeses for their proteolytic activity and capability to produce fermented milk enriched in angiotensin-converting enzyme (ACE)-inhibitory and antioxidant peptides. Preliminarily, 34 NSLAB from Parmigiano Reggiano (PR) and 5 from Pecorino Siciliano cheeses were screened based on their capacity to hydrolyze milk proteins. Two NSLAB strains from PR, Lactobacillus casei PRA205 and Lactobacillus rhamnosus PRA331, showed the most proteolytic phenotype and were positively selected to inoculate sterile cow milk. The fermentation process was monitored by measuring viable cell population, kinetic of acidification, consumption of lactose, and synthesis of lactic acid. Milk fermented with Lb. casei PRA205 exhibited higher radical scavenging (1184.83 ± 40.28 mmol/L trolox equivalents) and stronger ACE-inhibitory (IC50 = 54.57 μg/mL) activities than milk fermented with Lb. rhamnosus PRA331 (939.22 ± 82.68 mmol/L trolox equivalents; IC50
Methods Clinical strains of lactobacilli were freshly isolated from urogenital tract samples of young and adolescent patients using growth on Lactobacillus MRS Agar (HiMedia) and variant of Shaedler medium. Additional differential indication media were used. Bacterial growth was studied in the presence of disc- or drop- applied of LL on solid media or LL within bacterial suspensions (0.5-5 McFarland units). Samples studied were characterized with lactobacilli at the level of 106-108 Cfu/ml, and did not contain a panel of standard bacterial and viral pathogens. Decreased contents of Staphylococcus ssp., Enterococcus ssp., E. coli, and Candida ssp. were registered.. ...
The aim of this study is to establish the microbiological stability of traditionally fermented permanent sausages of domestic pig and wild boar meat mixtures. A sausage production mixture was inoculated with the strain Lactobacillus curvatus MRS_532 which has shown a good technological potential in the previous studies and is selected for use as a starter culture. During fermentation and ripening the sausage was followed by survival of the inoculated strain, as well as its influence on the microbiological composition of sausages, compared to the uninoculated control. The pH value was determined by a significant difference in the pH value of the inoculated sausage after 7. (p ,0,001) and 40 days (p ,0,05) compared to uninoculated sausage. The number of lactobacilli in inoculated sausage was significantly higher than the number of lactobacilli in the control sausage during all phases (p ,0,05), while the number of unwanted microbial generally fell towards the end of ripening. Based on the analysis ...
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Widespread use of antibiotics in the intensive care unit is a potential cause of the emergence of hospital-acquired pneumonia. This study determined whether Lactobacillus salivarius feeding could reverse antibiotic-induced lung defense impairment in a ventilator model. C57BL/6 wild-type (WT) mice received mechanical ventilation for 3 h after intramuscular antibiotic treatment for 6 days. Treatment with dead Lactobacillus salivarius and fructo-oligosaccharides (FOS) feeding were used to stimulate antibacterial protein expression in the intestine. Reactive oxygen species (ROS) in the intestinal mucosa was detected using 2ʹ7ʹ-dichlorofluorescein diacetate. The peroxynitrite production of alveolar macrophages (AMs) was measured using dihydrorhodamine 123 oxidation assay. N-acetylcysteine (NAC), an ROS scavenger, was orally administered to mice receiving antibiotics with FOS feeding. Antibiotic treatment decreased Pseudomonas aeruginosa (PA) phagocytic activity and activity of AMs and protein expression of
Cereal grains are often included in equine diets. When starch intake exceeds foregut digestion starch will reach the hindgut, impacting microbial ecology. Probiotics (e.g., lactobacilli) are reported to mitigate GI dysbioses in other species. This study was conducted to determine the effect of exogenous lactobacilli on pH and the growth of amylolytic and lactate-utilizing bacteria. Feces were collected from 3 mature geldings fed grass hay with access to pasture. Fecal microbes were harvested by differential centrifugation, washed, and re-suspended in anaerobic media containing ground corn, wheat, or oats at 1.6% (w/v) starch and one of five treatments: Control (substrate only), L. acidophilus, L. buchneri, L. reuteri, or an equal mixture of all three (107 cells/mL, final concentration). After 24 h of incubation (37°C, 160 rpm), samples were collected for pH and enumerations of total amylolytics, Group D Gram-positive cocci (GPC; Enterococci, Streptococci), lactobacilli, and lactate-utilizing bacteria.
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Scanning electron micrograph (SEM) of Lactobacillus buchneri, Gram-positive, non-spore forming, anaerobic or microaerophilic, rod prokaryote. This bacterium is a heterofermentative bacterium that produces lactic acid and acetic acid during fermentation. When silages are exposed to air, yeasts can degrade lactic acid, which increases the pH and leads to spoiling. L. buchneri is used as a bacterial inoculant to improve the aerobic stability of silage (grass silage, legume silage and grain silage, high moisture grains). L. buchneri is a heterolactic microbe that produces high concentrations of acetic acid, and sometimes lesser amounts of 1, 2 propanediol, propanol, and propionic acid. Both acetic acid and propionic acid are more effective at reducing the growth of yeasts and molds than is lactic acid during silage storage. Magnification: x1,400 when shortest axis printed at 25 millimetres. - Stock Image C037/0129
Vaginal lactic acid bacteria defend the host against pathogens through a combination of competitive exclusion, competition for nutrients, production of antimicrobial substances and through the activation of the immune system. A new human isolate named Lactobacillus crispatus L1 was characterized in this work, and a preliminary evaluation of its probiotic potential is described together with a process to obtain a high productivity of viable biomass. In a simulated digestion process 1.8⋅1010 cells∙ml−1 survived the gastric environment with 80% viability, without being affected by small intestine juices. Experiments on six different C sources were performed to analyze growth and organic acids production and, glucose, provided the best performances. A microfiltration strategy was exploited to improve the cellular yield in 2 L-fermentation processes, reaching 27 g · l−1 of dry biomass. Moreover, L. crispatus L1 demonstrated a greater stability to high concentrations of lactic acid, compared to other
This study aimed to assess the antibiotic susceptibility of Lactobacillus strains of goat milk origin. Lactobacilli are generally recognized as safe, but can act as reservoir for genes carrying antibiotic resistance traits; which can further spread to commensal, food spoilage or pathogenic organisms. Lactic acid bacteria (LAB) strains coming from animal sources had significant interaction with antibiotics. Goat milk, being second to cow milk in production and due to its close resemblance to human milk composition, is among favorable source for isolating potential probiotic LAB strains. Herein, this study assessed the antibiotic susceptibility pattern of fourteen Lactobacillus strains isolated from goat milk. The antibiotic susceptibility was recorded against thirty antibiotics following standard disk-diffusion assay. Imipenem, meropenem and nitrofurantoin turned out to be the most effective antibiotics, displaying high zone of inhibition (ZOI) against all the isolates. In contrast, all
A prospective, placebo-controlled, triple blind clinical trial was carried out in Pakistan to determine the effect of Lactobacillus GG on the course of acute diarrhea in hospitalized children. Forty children (mean age, 13 months) were enrolled and after rehydration received either oral Lactobacillus GG (n = 21) or placebo (n = 19) twice daily for 2 days, in addition to the usual diet. The clinical course of diarrhea was followed during the treatment period. Features on admission into the study groups were similar and were characterized by severe diarrhea, malnutrition and inappropriate management before presentation. Response was evident on Day 2 when the frequency of both vomiting and diarrhea was less in the Lactobacillus group. In those who had presented with acute nonbloody diarrhea (n = 32), the percentage of children with persistent watery diarrhea at 48 hours was significantly less in the Lactobacillus group: 31% vs. 75% (P | 0.01). No significant difference was observed by 48 hours in those
Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmun …
En el presente estudio se aisló, caracterizó y evaluó cepas de Lactobacillus sp con potencial probiótico a partir de pan de abejas de Apis mellifera; las cepas fueron incorporadas como bacterias probioticas mediante fermentación in vitro en polen apícola para así lograr un potencial producto funcional. Se procesaron 38 muestras de pan de abejas en el medio de cultivo, Man Rogosa Sharpe (MRS), se realizó pruebas bioquímicas para su identificación. Entre las pruebas para evaluar la capacidad probiótica se determinó: tolerancia a pH ácido (3, 4 y 5), crecimiento en bilis (0.3, 0.5 y 1.0%), actividad hemolítica, actividad antibacteriana frente a cepas de referencia y sensibilidad a antibióticos; e identificación molecular. Se obtuvieron 11 aislamientos de Lactobacillus sp con una o más características probióticas, 4 de ellas resistente a pH bajos y a bilis, productoras de sustancias antimicrobianas, no productoras de hemolisinas y sensibles a antibióticos comunes como amoxicilina ...
Adherence is a critical first step in the infection of a new host for many pathogens, including N. gonorrhoeae. As incoming pathogens, gonococci have to overcome the hosts defenses and compete with the indigenous microbiota to effectively colonize the endocervical epithelia. L. jensenii, one of the bacterial species most commonly isolated from the healthy human vaginal tract, has been shown to inhibit gonococcal adherence to and invasion of epithelial cells using a coculture model of infection (38). The inhibition of gonococcal adherence was not caused by any secreted factor, such as hydrogen peroxide, by coaggregation of the gonococci and the lactobacilli, or by competition for receptors. The ability to inhibit gonococcal adherence without direct killing of the pathogen might be specific to Lactobacillus species, as we found no inhibition of adherence when cells were pretreated with another Gram-positive bacterium, B. subtilis. This finding correlates with research which showed that vaginal ...
Ingredients: (INCI): Sorbitol, Propylene Glycol, Aqua, Sodium Stearate, Sodium Laureth, Sulphate, Sodium Laurate, Sodium Laureth 13 Carboxylate, Sodium Chloride, Glycerine, Caprilic/ Capric Triglyceride, Tetrasodium EDTA, Probiotic, Lactobacillus bulgaricus, 21 amino acids, Panax Ginseng Extract ...
Lactobacillus Paracasei Market, By Type (Pharmaceutical Grade, Food Grade, Other), By End-Users (Dairy Products, Healthy Food, Drink, Pharmaceutical, Dietary Supplements, Other), By Region (North America, Europe, Asia Pacific, Middle East & Africa, and So
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
TY - JOUR. T1 - Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis. AU - Garde, Arvid. AU - Jonsson, Gunnar Eigil. AU - Schmidt, A. S.. AU - Ahring, B. K.. PY - 2002. Y1 - 2002. N2 - Lactic acid production by Lactobacillus brevis and Lactobacillus pentosus on a hemicellulose hydrolysate (HH) of wet-oxidized wheat straw was evaluated. The potential of 11-12 g/l fermentable sugars was released from the HH through either enzymatic or acidic pretreatment. Fermentation of added xylose in untreated HH after wet-oxidation, showed no inhibition on the lactic acid production by either Lb. pentosus or Lb. brevis. Lb. pentosus produced lactate corresponding to 88% of the theoretical maximum yield regardless of the hydrolysis method, whereas Lb. brevis produced 51% and 61% of the theoretical maximum yield after enzymatic, or acid treatment of HH, respectively. Individually, neither of the two strains were able to fully utilize the relatively ...
Lactobacilli are normal inhabitants of the gastrointestinal tract of man and animals where they are widely considered to exert a number of beneficial roles including immunomodulation, interference with enteric pathogens, and maintenance of a healthy intestinal microflora. Historically, probiotic roles have been ascribed primarily to Lactobacillus acidophilus. The genus Lactobacillus presently comprises more than 50 recognized species of non-pathogenic bacteria which in addition to their probiotic effects are useful to human as indispensable agents for the fermentation of foods and feed. Lactobacillus amylovorus (strain GRL 1118) is an anaerobic, nonmotile, non-spore-forming, and rod-shaped Gram-positive bacterium isolated from porcine ileum and which shows strong adherence to pig intestinal epithelial cells. L. amylovorus is an abundant Lactobacillus species found in the intestines of piglets that has exhibited several potential probiotic properties, such as antimicrobial activity against ...
Lactobacillus delbrueckii subsp. bulgaricus is one of the economically most important representatives of the heterogeneous group of lactic acid bacteria, with a worldwide application in yogurt production. Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) was originally isolated from bulgarian yogurt in 1919. L.bulgaricus belongs to the acidophilus complex and is considered unique within this group because of its atypical GC content. Its overall GC content differs significantly from related species such as L.acidophilus and L.johnsonii. This is mainly due to important differences at codon position 3. L.bulgaricus contains a relatively high number of pseudogenes, 270, suggesting that the genome is undergoing size reduction and gene elimination. A noncoding region of 2.5 kbp has been found that has all the features of a CRISPR region. The genome contains a high number of rRNA and tRNA genes, which seems to indicate that it has recently undergone a phase of size reduction. ...
0056] It is preferred that the probiotic mixture could contain any number of microorganisms and or microbial components, spores, and/or metabolites. Examples of bacterial species that could be used for the probiotic mixture include but are not limited to the group consisting of: Enterococcus faecium, Bacillus licheniformis, Lactococcus lactis, Bacillus subtilis, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermophilum, Lactobacillus acidophilus, Lactobacillus agilis, Lactobacillus alactosus, Lactobacillus alimentarius, Lactobacillus amylophilus, Lactobacillus amylovorans, Lactobacillus amylovorus, Lactobacillus animalis, Lactobacillus batatas, Lactobacillus bavaricus, Lactobacillus bifermentans, Lactobacillus bifidus, Lactobacillus brevis, Lactobacillus buchnerii, Lactobacillus bulgaricus, Lactobacillus catenaforme, Lactobacillus casei, Lactobacillus cellobiosus, Lactobacillus collinoides, ...
Lactobacilli are important for the maintenance of a healthy ecosystem in the human vagina. Various mechanisms are postulated but so far are poorly substantiated by molecular studies, such as mutant analysis. Bacterial autoaggregation is an interesting phenomenon that can promote adhesion to host cells and displacement of pathogens. In this study, we report on the identification of a human vaginal isolate, Lactobacillus plantarum strain CMPG5300, which shows high autoaggregative and adhesive capacity. To investigate the importance of sortase-dependent proteins (SDPs) in these phenotypes, a gene deletion mutant was constructed for srtA, the gene encoding the housekeeping sortase that covalently anchors these SDPs to the cell surface. This mutant lost the capacity to autoaggregate, showed a decrease in adhesion to vaginal epithelial cells, and lost biofilm-forming capacity under the conditions tested. These results indicate that the housekeeping sortase SrtA of CMPG5300 is a key determinant of the ...
TY - JOUR. T1 - Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats. AU - da Costa, Whyara Karoline Almeida. AU - Brandão, Larissa Ramalho. AU - Martino, Maria Elena. AU - Garcia, Estefânia Fernandes. AU - Alves, Adriano Francisco. AU - de Souza, Evandro Leite. AU - de Souza Aquino, Jailane. AU - Saarela, Maria. AU - Leulier, François. AU - Vidal, Hubert. AU - Magnani, Marciane. PY - 2019/10/1. Y1 - 2019/10/1. N2 - Tropical fruit and their industrial processing byproducts have been considered sources of probiotic Lactobacillus. Sixteen tropical fruit-derived Lactobacillus strains were assessed for growth-promoting effects using a host-commensal nutrient scarcity model with Drosophila melanogaster (Dm). Two Lactobacillus strains (L. plantarum 49 and L. plantarum 201) presenting the most significant effects (p ≤ .005) on Dm growth were selected and evaluated for their safety and ...
This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this ...
Lactobacillus delbrueckii subsp. bulgaricus ATCC ® 11842D-5™ Designation: Genomic DNA from Lactobacillus delbrueckii subsp. bulgaricus strain Lb14 TypeStrain=True Application:
The strain Lactobacillus fermentum ME-3 has recently been discovered and identified as an antimicrobial and antioxidative probiotic. This strain of Lactobacillus fermentum was discovered from the analysis of human fecal samples in 1994. One of the important characteristics of a probiotic microbe is the tolerance to conditions in the digestive tract. Tests conducted on the ME-3 strain in different bile concentrations found that it was able to survive without large loss in numbers. It has also been found that Lactobacillus fermentum ME-3 has a tolerance to survive drops of pH levels. It can withstand a drop in values from 4.0 to 2.5 without decreasing in numbers. These characteristics of tolerance to bile concentrations and pH levels serve to classify ME-3 as a probiotic.[10] Lactobacillus fermentum ME-3 has also been found to have the capability to suppress mainly gram-negative bacteria. To a lesser extent, ME-3 has also been observed to be able to suppress Enterococci and Staphylococcus aureus. ...
TY - JOUR. T1 - Probiotic Potential of Lactobacillus paracasei CT12 Isolated from Water Kefir Grains (Tibicos). AU - Romero-Luna, Haydee Eliza. AU - Peredo-Lovillo, Audry. AU - Hernández-Mendoza, Adrián. AU - Hernández-Sánchez, Humberto. AU - Cauich-Sánchez, Patricia Isidra. AU - Ribas-Aparicio, Rosa María. AU - Dávila-Ortiz, Gloria. PY - 2020/1/1. Y1 - 2020/1/1. N2 - © 2020, Springer Science+Business Media, LLC, part of Springer Nature. The water kefir grains are a multi-species starter culture used to produce fermented beverages of sucrose solution with or without fruit extracts. The water kefir grains are known in Mexico as Tibicos, which are mainly used to produce Tepache, a traditional Mexican drink made by fermenting pineapple peel. The microbiota of Tibicos mainly include lactic acid bacteria (LAB) and since most probiotics belong to this group, Tibicos may represent a potential source of probiotic bacteria. Moreover, several bacteria isolated from kefir samples have been ...
Figure 1S. Bove et al. Score plot of the first and second principal components (PC1 and PC2) after PCA of the profiles of fermentation of Lactobacillus rhamnosus strains as determined using the Biolog system. Before analysis, L. rhamnosus strains were cultivated on MRS (PR825M, PR826M, PR830M, PR1019M, PR1215M, PR1224M, PR1473M, PR1479M, PR1484M and PR1489M) and Cheese agar (PR825C, PR826C, PR830C, PR1019C, PR1215C, PR1224C, PR1473C, PR1479C, PR1484C and PR1489C) media at 30°C for 48 h.. Figure 2S. Bove et al. 2-DE analysis of intracellular and cell-associated proteins synthesized by Lactobacillus rhamnosus PR830 (A, C) and PR1215 (B, D) grown on MRS (A, B) and CB (C, D) until the stationary phase of growth (30°C for 24 and 48 h, respectively) was reached. Compared to cultivation on MRS, numbered triangles and circles refer to proteins that decreased or increased the amount during growth on CB. Numbered rectangles refer to proteins whose amount decreased or increased during growth on CB for at ...
Certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (BV), with Gardnerella vaginalis being the most common. However, the exact role of G. vaginalis in BV has not yet been determined. The main goal of this study was to test the hypothesis that G. vaginalis is an early colonizer, paving the way for intermediate (e.g., Fusobacterium nucleatum) and late colonizers (e.g., Prevotella bivia). Theoretically, in order to function as an early colonizer, species would need to be able to adhere to vaginal epithelium, even in the presence of vaginal lactobacilli. Therefore, we quantified adherence of G. vaginalis and other BV-associated bacteria to an inert surface pre-coated with Lactobacillus crispatus using a new Peptide Nucleic Acid (PNA) Fluorescence In Situ Hybridization (FISH) methodology. We found that G. vaginalis had the greatest capacity to adhere in the presence of L. crispatus. Theoretically, an early colonizer would contribute to the adherence and/or
Lactobacillus delbrueckii subsp. bulgaricus was first identified in 1905 by Stamen Grigorov, who named it Bacillus bulgaricus.[6] Ilya Metchnikoff, a professor at the Pasteur Institute in Paris, researched the relationship between the longevity of Bulgarians and their consumption of yogurt. He had the idea that aging is caused by putrefactive activity, or proteolysis, by microbes that produce toxic substances in the intestine. Proteolytic bacteria such as clostridia, which are part of the normal intestinal flora, produce toxic substances including phenols, ammonia and indols by digestion of proteins. These compounds are responsible for what Metchnikoff called intestinal auto-intoxication, which, according to him, was the cause of the physical changes associated with old age, a concept that has no scientific basis. It was already known at that time that fermentation with lactic acid bacteria inhibits the deterioration of milk because of its low pH. Metchnikoffs research also noted that rural ...
363879028 - EP 2457992 A1 20120530 - NOVEL LACTOBACILLUS PLANTARUM AND COMPOSITION COMPRISING SAME - The present invention provides Lactobacillus plantarum CJLP133 KCTC 11403BP, a composition for treating intestinal diseases comprising the lactic acid bacteria, and a composition for enhancing immunity comprising the lactic acid bacteria.[origin: WO2011010770A1] The present invention provides Lactobacillus plantarum CJLP133 KCTC 11403BP, a composition for treating intestinal diseases comprising the lactic acid bacteria, and a composition for enhancing immunity comprising the lactic acid bacteria.
Objective. To evaluate the safety of the antimicrobial peptide, lactocin 160. Methods. Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI) model was used for an in vivo safety evaluation. Results. The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9). Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis). Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vaginal irritation. An average irritation index grade was evaluated as none. Conclusions. Lactocin 160 showed minimal ...
Today the lactic acid bacteria (LAB) are routinely used as starter cultures in fermented food production; species Lactobacillus plantarum is used mostly for vegetables and olives conservation, ensiling and malolaclic fermentation of wines. In this work the influence of Lb. plantarum L4 biomass preparation for longterm preservation by freezing at different temperatures on physiological activity (i.e., viability and metabolic activity) was investigated. After growth in optimal conditions the bacterial biomass was prepared for freezing in the following ways: a) biomass sediment obtained after centrifugation was resus-pended in the whole volume of neutralized spent growth medium (BNNM); b) biomass sediment was resuspended in 1/5 th of spent growth medium volume (CB), and c) after centrifugation the bacterial biomass was rinsed and then resuspended in the physiological saline until reaching cell concentration as in a) MB). During 100 days of storage at -20, -30 and -70 °C the viability of cells by ...
Read Physical mapping and partial genetic characterization of the Lactobacillus delbrueckii subsp. bulgaricus bacteriophage lb539, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Vedecké štúdie o účinkoch kmeňa baktérie Lactobacillus reuteri DSM17648. Väčšina vedeckých skúmaní baktérie Lactobacillus reuteri bola venovaná jej účinkom na posilnenie, či ozdravenie tráviaceho traktu. Vedecké štúdie boli tiež venované možnému potenciálnemu účinku Lactobacillus reuteri na podporu liečby baktérie helikobakter pylori. Zoznámte sa s výsledkami niektorých z uskutočnených vedeckých štúdií:. Štúdia The effect of Lactobacillus reuteri supplementation in Helicobacter pylori infection: a placebo-controlled, single-blind study (Martin Buckley, Sean Lacey, Andrea Doolan, 2018). Štúdia bola vykonaná na 24 dospelých s diagnostikovaným h.pylori. Po 28-mich dňoch užívania baktérie Lactobacillus reuteri kmeň DSM17648 v produkte Pyllopas boli pacientom pomocou dychového testu merané zmeny na koncentrácii baktérie helikobakter pylori. Trend znižovania prítomnosť baktérie sa preukázal u 62,5% pacientov. Počas štúdie neboli ...
TY - JOUR. T1 - Characterization of Gene Encoding Amylopullulanase from Plant-Originated Lactic Acid Bacterium, Lactobacillus plantarum L137. AU - Kim, Jong Hyun. AU - Sunako, Michihiro. AU - Ono, Hisayo. AU - Murooka, Yoshikatsu. AU - Fukusaki, Eiichiro. AU - Yamashita, Mitsuo. PY - 2008/11. Y1 - 2008/11. N2 - A starch-hydrolyzing lactic acid bacterium, Lactobacillus plantarum L137, was isolated from traditional fermented food made from fish and rice in the Philippines. A gene (apuA) encoding an amylolytic enzyme from Lactobacillus plantarum L137 was cloned, and its nucleotide sequence was determined. The apuA gene consisted of an open reading frame of 6171 bp encoding a protein of 2056 amino acids, the molecular mass of which was calculated to be 215,625 Da. The catalytic domains of amylase and pullulanase were located in the same region within the middle of the N-terminal region. The deduced amino acid sequence revealed four highly conserved regions that are common among amylolytic enzymes. ...
TY - JOUR. T1 - Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. AU - Krooneman, J. AU - Faber, F. AU - Alderkamp, A C. AU - Elferink, S J H W Oude. AU - Driehuis, F. AU - Cleenwerck, I. AU - Swings, J. AU - Gottschal, J C. AU - Vancanneyt, M. PY - 2002/3. Y1 - 2002/3. N2 - Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence ...
Lactobacillus pentosus TV35b, isolated from the posterior fornix secretions of the vagina of a prenatal patient, produced a bacteriocin-like peptide (pentocin TV35b), which is inhibitory to Clostridium sporogenes, Cl. tyrobutyricum, Lact. curvatus, Lact. fermentum, Lact. sake, Listeria innocua, Propionibacterium acidipropionici, Propionibacterium sp. and Candida albicans. The mechanism of activity of pentocin TV35b is bactericidal, as shown by a decrease in the viable cell numbers of Lact. sake from approximately 4 x 108 to less than 10 cfu ml-1 over a period of 4 h. Pentocin TV35b added to the growth medium of C. albicans stimulated the formation of pseudohyphae during the first 36 h, followed by a slight repression in cell growth. Production of pentocin TV35b was at its maximum towards the end of the logarithmic growth phase of strain TV35b. The peptide was purified by ammonium sulphate precipitation, followed by SP-Sepharose cation exchange chromatography. The molecular size of pentocin TV35b ...
Natural Factors Ultimate Probiotic Improve uncomfortable, unhealthy digestion with this powerful probiotic. Ideal for anyone with a history of antibiotic treatment or a high toxic load. Contains 12 billion live micro-organisms per capsule. Supplement Facts: Amount Per Serving Serving Size 1 V capsule Servings per Container 60 A Proprietary Blend Lactobacillus casei - 3 billion Lactobacillus rhamnosus - 1.4 billion Lactobacillus acidophilus - 1.2 billion Lactococcus plantarum - 1.2 billion Lactobacillus rhamnosus(B) - 1.2 billion Lactococcus breve - 1.2 billion Bifidobacterium longum - 1.2 billion Bifidobacterium bifidum - 0.6 billion Lactobacillus lactis - 0.6 billion Lactococcus delbruecki subsp. Bulgaricus - 0.1 billion Lactobacillus helveticus - 0.1 billion Lactobacillus salivarius - 0.1 billion Other Ingredients: Vegetarian Capsule (cellulose, purified water), magnesium stearate (vegetable grade), FOS (fructooligosaccharides) inulin, ascorbic acid. Contains trace amounts of
Minuman lactobacillus yang banyak dijual di pasaran dan yoghurt ternyata punya perbedaan. Menurut dr. Carmen. M. Siagian, MS, Bagian Ilmu Gizi Fakultas Kedokteran, Universitas Kristen Indonesia, Jakarta. , dalam proses pembuatannya, minuman lactobacillus hanya menggunakan satu bakteri yaitu Lactobacillus bulgaricus. Sedangkan prinsip pembuatan yoghurt adalah fermentasi susu dengan menggunakan bakteri Lactobacillus bulgaricus dan Streptococcus thermophilus.…
Children: Lactobacillus is LIKELY SAFE when taken by mouth appropriately in children.. Pregnancy and breast-feeding: Lactobacillus is POSSIBLY SAFE when taken by mouth appropriately while pregnant and breast-feeding.. Weakened immune system: There is some concern that lactobacillus from supplements that contain live bacteria might grow too well in people whose immune systems are weakened. This includes people with HIV/AIDS or people who have taken medicines to prevent rejection of a transplanted organ. Lactobacillus has caused disease (rarely) in people with weakened immune systems. To be on the safe side, if you have a weakened immune system, talk with your healthcare provider before taking lactobacillus.. Short bowel syndrome: People with short bowel syndrome might be more likely than other people to develop lactobacillus infections. If you have this condition, talk with your healthcare provider before taking lactobacillus.. Ulcerative colitis: People with ulcerative colitis that is severe ...
Background and objectives: Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated Lactobacillus was investigated. Materials and Methods: The 16S rDNA technology was used for microbial identification. A mouse constipation model was established using activated carbon. After intragastric administration of Lactobacillus (109 CFU/mL), the mice were dissected to prepare pathological sections of the small intestine. Serum indicators were detected using kits, and the expression of small intestine-related mRNAs was detected by qPCR assay. Results: One strain of Lactobacillus was identified and named Lactobacillus fermentum CQPC03 (LF-CQPC03). Body weight and activated carbon propulsion rate were all higher in mice intragastrically administered with LF-CQPC03 compared with the control group, while the time to the first black stool in treated mice
Medicinal Ingredients: Each capsule contains:Lactobacillus rhamnosus (ATCC 7469) 800 million cfu, Bifidobacterium bifidum (ATCC 15696) 600 million cfu, Lactobacillus acidophilus (4356) 600 million cfu, Lactobacillus plantarum (ATCC 14917) 600 million cfu, Bifidobacterium infantis (ATCC 15697) 400 million cfu, Bifidobacterium longum (ATCC 15707) 400 million cfu, Lactobacillus casei (ATCC 393) 400 million cfu, Lactobacillus salivarius (ATCC 11741) 400 million cfu. Non-medicinal Ingredients: Gelatin capsule, inulin, cellulose, magnesium stearate (vegetable source), fructooligosaccharides, lactobacillus bulgaricus, streptococcus thermophiles, lactobacillus helveticus.. ...
This is equivalent to 10,000,000,000 CFU/g.. Lactobacillus Plantarum Weight Loss BiOptimizers will help you fight the free radicals that damage your body on a daily basis. If your consumption of foods rich in nutrients is low, you will need to supplement your diet with this P3-OM supplement. Since it helps in such important aspects of the body, it also aids in preventing premature aging from occurring. It will prevent the premature aging of our body and maintain an enviable health.. Lactobacillus Plantarum Weight Loss by BiOptimizers is an effective supplement that works when you consume the right amount. The probiotic properties of the Lactobacillus Plantarum Weight Loss ingredients work like every other supplement in the market, when you take the supplement the healthy bacteria enter your digestive tract and start work. These good bacteria go down to your GI tract and attach themselves to your intestines, from here they start to clean your body and fight against any illness. Lactobacillus ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
TY - JOUR. T1 - Hypolipidemic effect of lactobacillus ferment as a functional food supplement. AU - Choi, Y. M.. AU - Bae, S. H.. AU - Kang, D. H.. AU - Suh, H. J.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2006/12. Y1 - 2006/12. N2 - The Lactobacillus ferment used in this study was composed of Lactobacillus fermented wheat, barley and kefir grains. Fermentation increased the CFU of lactic acid bacteria with a reduction in pH value and in the contents of dietary fiber and glucan. Male SD rats were fed a high fat diet with or without 10% Lactobacillus ferment for 4 weeks. In the Lactic-F group (group fed high-fat diet with Lactobacillus ferment) there was a significantly reduced increase of body weight compared with the HF-control (group fed high-fat diet without Lactobacillus ferment). The food efficiency ratio (FER) tended to be decreased in the Lactic-F group, but there was no significant difference between the Lactic-F and HF-control groups. The perirenal and ...
Influence of soymilk and skim milk on growth and antibacterial activity of lactic acid bacteria biogenic amine;lactic acid bacteria;skim milk;soymilk; The purpose of this study was to investigate the effect of lactic acid bacteria (LAB) on the growth and biogenic amines (BA) formation of Enterobacter aerogenes CIH05 in skim milk and soymilk. Lactobacillus acidophilus GK20, Lactobacillus paracasei GK74, and Lactobacillus plantarum GK81 isolated from mustard kimchi did not produce BA in the decarboxylation broth. L. paracasei GK74 exhibited the highest cell viability and antimicrobial compounds producing ability in fermented skim milk and soymilk samples, while the lowest producer was L. plantarum GK81. The production yield of lactic acid, hydrogen peroxide, and bacteriocin was dependent on the species of Lactobacillus and the type of culture medium. As LAB the number of viable cells of E. aerogenes CIH05 were higher in skim milk than in soymilk. When mixed culture with L. acidophilus GK20 and L.
The present study aims at comparing the performances of three Lactobacillus reuteri strains (DSM 20016, DSM 17938, and ATCC 53608) in producing 3-hydroxypropionic acid (3-HP) from glycerol and at exploring inhibition phenomena during this bioconversion. Differences were highlighted between the three strains in terms of 3-HP production yield, kinetics of substrate consumption, and metabolite production. With a maximal productivity in non-optimal conditions (free pH) around 2 g.L−1.h−1 of 3-HP and 4 g.L−1.h−1 of 3-hydroxypropionaldehyde (3-HPA) depending on the strain, this study confirmed the potential of L. reuteri for the biotechnological production of 3-HP. Moreover, the molar ratios of 3-HP to 1,3-propanediol (1,3-PDO) obtained for the three strains (comprised between 1.25 and 1.65) showed systematically a higher 3-HP production. From these results, the DSM 17938 strain appeared to be the most promising strain. The impact of glycerol bioconversion on the bacterias physiological state (a
Genetic diversity and genomic rearrangements are a driving force in bacterial evolution and niche adaptation. We sequenced and annotated the genome of Lactobacillus johnsonii DPC6026, a strain isolated from the porcine intestinal tract. Although the genome of DPC6026 is similar in size (1.97mbp) and GC content (34.8%) to the sequenced human isolate L. johnsonii NCC 533, a large symmetrical inversion of approximately 750 kb differentiated the two strains. Comparative analysis among 12 other strains of L. johnsonii including 8 porcine, 3 human and 1 poultry isolate indicated that the genome architecture found in DPC6026 is more common within the species than that of NCC 533. Furthermore a number of unique features were annotated in DPC6026, some of which are likely to have been acquired by horizontal gene transfer (HGT) and contribute to protection against phage infection. A putative type III restriction-modification system was identified, as were novel Clustered Regularly Interspaced Short ...
BBXSWL - Sour Weapon L (Lactobacillus plantarum Blend) - the second member of our Sour Weapon™ family. Commercial Pitch Sizes Available: 1BBL, 2BBL, 5BBL, 10BBL, 15BBL, 30BBL. Source: Traditional Norwegian Kveik brewers.. The new De Lacto Standard! Our first official Lactobacillus culture, and its a beast. Weve tested countless LAB cultures over the years, and this blend of Lactobacillus plantarum is the most brutally effective Lactic Acid producer weve found!. If youre looking to drop the pH of wort as quickly and low as possible, Sour Weapon L is your go to Lacto blend. At 98F, weve had trial batches drop the pH of wort to 3.0 after just 24 hours. When pitched at 84F, pH should reach 3.5 in 24 hours.. This is the ideal bacteria blend to use for acidifying wort for quick/kettle sours, and is also very effective when co-pitched with a yeast strain. As with any Lactobacillus culture, we recommend only using in wort with zero IBUs as even small amounts of hops may prevent significant ...
BBXSWL - Sour Weapon L (Lactobacillus plantarum Blend) - the second member of our Sour Weapon™ family. Availability: Limited. Source: Traditional Norwegian Kveik brewers.. The new De Lacto Standard! Our first official Lactobacillus culture, and its a beast. Weve tested countless LAB cultures over the years, and this blend of Lactobacillus plantarum is the most brutally effective Lactic Acid producer weve found!. If youre looking to drop the pH of wort as quickly and low as possible, Sour Weapon L is your go to Lacto blend. At 98F, weve had trial batches drop the pH of wort to 3.0 after just 24 hours. When pitched at 84F, pH should reach 3.5 in 24 hours.. This is the ideal bacteria blend to use for acidifying wort for quick/kettle sours, and is also very effective when co-pitched with a yeast strain. As with any Lactobacillus culture, we recommend only using in wort with zero IBUs as even small amounts of hops may prevent significant souring.. Ordering Information:. Please note that ...
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Introduction: Application of lactic acid bacteria for synthesis of silver (AG) nanoparticles (NPs) could be a good ecological friendly alternative to chemical and physical methods. The objective of this study was to investigate the biosynthesis of silver NPs using Lactobacillus strains and to compare their monosaccharide composition of capsular exopolysaccharides and the antibacterial activity of synthesized nanoparticles. Methods: The washed cells of 22 Lactobacillus strains were used for in vitro silver nanoparticle biosynthesis from silver nitrate solution. The NPs formation was confirmed by UV-visible spectroscopy and transmission electron microscopy (TEM) analysis. TEM micrographs were used for the evaluation of NPs size. The monosaccharide composition of capsular exopolysaccharides was determined using GC/MS analysis. The antimicrobial activity was determined by agar well diffusion assay. Results: The capsular layers of Lactobacillus strains contained heteropolysaccharides that were composed
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PREVENTAVIR - antiviral immunostimulant is based on lactobacillus helveticus - it may help to increase the immune response to the RNA viral load (invasion). Buy online PREVENTAVIR. Worldwide shipping.
A large number of strains of bacteria is not always beneficial and justified, i.e. in the case of probiotic bacteria a large variety does not always equal a better pro-health effect. Despite the enormous natural diversity of microorganisms in the gastrointestinal tract, it is estimated that in healthy people there are about 4 species of bacteria of the genera Bifidobacterium sp. and Lactobacillus sp. and Lactobacillus sp. Of course, this does not mean that each of us has the same 4 species of bacteria from the genera Lactobacillus sp. and Bifidobacterium sp. in our intestines, because there is the so-called individual variability. In practice, this means that we isolate different species of bacteria belonging to the genera Lactobacillus sp. and Bifidobacterium sp. from individuals. It is also more important to select such representatives of these rows so that their properties are desired and not their number. We must remember that the properties of a given bacterium do not depend on their ...
The gastrointestinal apparatus hosts a bacterial population that quantifies to around 1011 microorganisms per gram of content with more than 400 different species. Its principal functions are: metabolic, trophic, and protective. The Lactobacillus reuteri (Reuterin®) is considered one of the few native species in the human intestinal tract. It is able to modulate the immune response CD4+ T-Helper to an ileum level. Lactobacillus reuteri isolated for the first time in 1980 is a heterofermantative species. Its probiotic activity is attributed to the ability to exercise an inhibitive effect on the pathogenic micro-organisms with a combination of mechanisms including the production of lactic acid, hydrogen peroxide, antimicrobic substances and bactericide. Moreover, Lactobacillus reuteri is in a position to produce various short chain-like fat acids such as the acetic acid from the fermentation of carbohydrates. At last, it is in a position to produce a powerful antimicrob substance known as ...