Animal models for carcinogenesis of the oral cavity are limited, although this disease is often fatal or disfiguring and its incidence in the USA is ~30 000 cases/year. Short-term whole-animal models for this disease should prove valuable in the investigation of factors affecting oral carcinogenesis. In this study we observed that a group of oral carcinogens are clearly mutagenic in the lacZ transgenic mouse oral cavity. The carcinogens 4-nitroquinoline-N-oxide (4-NQO), benzo[a]pyrene (B[a]P), N-nitroso-N-methylurea (NMU), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), nitrosonornicotine (NNN) and 7,12-dimethylbenzanthracene (DMBA) were all mutagenic in a mixture of pooled oral tissues (gingival, buccal, pharyngeal and sublingual) and in the tongue. All agents except DMBA (which was swabbed in the oral cavity) and B[a]P (by gavage) were given in drinking water for 2-4 weeks followed by a 2 week expression period before killing. With one exception, groups of 4-5 female mice were treated. ...
lac repressor binds simultaneously to two sites near the promoter of the lac operon. Repressor-binding sites are called operators. One operator (O1) is adjacent to the promoter, and the other (O2) is within the coding region of lacZ. When bound to both operators, the repressor causes the DNA to form a stable loop that can be seen in electron micrographs of the complex formed between lac repressor and DNA (bottom figure). The interaction of lac repressor with the operator sequences may block transcription by preventing the binding of RNA polymerase to the lac promoter. However, it is now known that, in some cases, both lac repressor and RNA polymerase can bind to the promoter at the same time. Thus, the repressor may also block transcription initiation by preventing formation of the open complex and promoter clearance. A schematic diagram of lac repressor bound to DNA in the presence of RNA polymerase is shown in the figure on the right. [See Mondays Molecule #61 for another view.] The diagram ...
Section L:Regulation of transcription in prokaryotes. Molecular Biology. L1 The lac operon L2 The trp operon L3 Transcriptional regulation by alternative σ factors. Molecular Biology. L1:The LAC Operon. Operon - what is it? Slideshow 6150074 by otto-barker
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TY - JOUR. T1 - Mutation frequency and type during ageing in mouse seminiferous tubules. AU - Martin, Sandra L.. AU - Hopkins, Craig L.. AU - Naumer, Anne. AU - Dollé, Martijn E T. AU - Vijg, Jan. PY - 2001. Y1 - 2001. N2 - Mutations arise in the germline by errors of replication, recombination and repair, and the movement of transposable elements. Transgenic mice bearing reporter genes such as lacZ have proven useful for measurements of spontaneous and induced mutation frequencies, as well as studies of the effects of ageing. In this study, testicular DNA from lacZ transgenic mice was examined for age-related effects on mutation frequency and type. The recovered transgene was tested for simple substitutions and rearrangements including transposition of endogenous mobile elements. There was no evidence for either an age-related accumulation of mutations, or for the insertion of retrotransposons into the lacZ reporter gene in the testis. We conclude that the frequency of retrotransposition of ...
Advances in understanding the role of dendritic cells (DCs) as the major antigen (Ag)-presenting cell type of the immune system combined with the recent development of methods for the ex vivo expansion of human DCs have opened the possibility for the transfer of tumor Ags to DCs with a view toward tumor immunotherapy. In this study, we examined the feasibility of Ag transfer to cultured human DCs using the host range-restricted avipoxvirus, fowlpoxvirus (FWPV). FWPV was found to infect and express a lacZ marker gene in a number of mammalian cell lines of fibroblastic, epithelial, and hemopoietic lineage origins. LacZ recombinant FWPV (rFWPV) was found subsequently to infect human DCs that had been cultured ex vivo from peripheral blood monocytes. Using rFWPV containing lacZ under the control of a vaccinia virus (VV) early/late promoter (p7.5K) and a 10 plaque-forming units per cell multiplicity of infection, ,80% of cells expressed the lacZ marker gene. Quantitative analysis showed that the ...
Having demonstrated that PEMF has a potent effect on endothelial cells in vitro, we examined whether PEMF was able to stimulate angiogenesis in vivo. Matrigel is a soluble basement membrane preparation, and when implanted s.c. supports vascular ingrowth. Matrigel was injected s.c. into tie2/lacZ transgenic mice that were housed in cages emitting PEMF for 8 h a day or control cages. After 3, 10, and 14 days, there was significantly greater vascular ingrowth into the matrix in PEMF-treated animals, confirmed by staining specific for endothelial markers CD31 and Tie-2. PEMF increased the vascular ingrowth more than twofold by day 3 (13.3±0.41 vs. 5.8±0.28 cells/hpf; P,0.01). This increase in vascular ingrowth persisted through days 10 and 14 (16.6±0.49 vs 12.6±0.43 cells/hpf; P,0.01, and 19.4±0.55 vs. 14.8±0.40 cells/hpf; P,0.01, respectivelLISA confirmed a twofold increase in FGF-2 in PEMF-treated matrigel, but demfactors TPO, Ang-2, and EGF (data not shown). In this study, we demonstrate ...
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT The nucleotide sequence of the lac operator; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT The nucleotide sequence of the lac operator; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
We describe several new vectors for the construction of operon and protein fusions to the Escherichia coli lacZ gene. In vitro constructions utilize multicopy plasmids containing suitable cloning sites located between upstream transcription terminators and downstream lac operon segments whose lacZ g …
BACKGROUND AND OBJECTIVE: Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS: To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ, a marker gene used here as a model, in the presence of IL-3, IL-6, GM-CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM-CSF and TNF-a. RESULTS: Addition of IL-4 was found to induce dendritic differentiation preferentially by inhibiting proliferation and differentiation of the macrophage lineage. As assessed by X-Gal staining, LacZ gene expression was observed in cells from both the dendritic lineage (CD1a+/CD14-) which still exhibits the highest immunostimulatory activity in mixed ...
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Not sure why I cant edit the above part, but I stumbled across something that seemed to be of use; that secondary sugars might be sugars the require cAMP and CRP to be the effector of the operon(along with the operon inducer of course). Since gallactose is what the cell wants the most then the other metabolic operons(like lactose) should be off so any sugar that isnt gallactose is secondary ...
Group of adjacent and coordinately controlled genes concerned with the metabolism of lactose in E. coli. The lac operon was the first example of a group of genes under the control of an operator region to which a lactose repressor binds. When the
The natural Lac-operon has 2 controls that tightly regulate the production of the proteins necessary for the breakdown of lactose. In the presence of glucose, the Lac Operon inhibits the production of those proteins. When glucose is present, the lac repressor is bound to the operator, prohibiting the transcription of the proteins. In the presence of lactose, lactose is able to bind to the lac repressor initiating a conformation change in the repressor protein, causing it to release and allow for transcription. This, however, is not the only control in place. It is incredibly energy intensive to produce the proteins necessary for the breakdown of lactose, so when there is glucose present, the glucose will be metabolized first, even with some lactose present. This is accomplished by the presence of a second regulatory device, cyclic AMP (cAMP). cAMP is present only when there are very low levels of glucose found in the environment. cAMP serves as an activator and binds to RNA polymerase allowing ...
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Copyright Get Revising 2018 all rights reserved. Get Revising is one of the trading names of The Student Room Group Ltd. Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered office: International House, Queens Road, Brighton, BN1 3XE ...
The geologic characteristics for the Lac Cinquante, J4 and Ray deposits are very similar. Separated by only 1,800 metres and exhibiting the same general trends, it is likely that these three deposits represent the same, or related mineralized structures. Deposits in the Lac 50 Trend are basement hosted, vein-hydrothermal type. The emplacement of uranium is structurally controlled, often associated with a graphite-chlorite tuffaceous metasediment interlayered in Archean metavolcanics. Mineralization consists of disseminated and patchy pitchblende with sulphides within fracture controlled, brecciated, hematite-quartz-carbonate veins that can occur in either the host shear or tuff unit. Potential also exists for unconformity-style uranium deposits to occur on the Angilak Property. The Lac Cinquante deposit is comprised of three distinct mineralized sections occurring over 3.8 kilometres of strike length and interpreted to be off-set by faults. The central Main Lac Cinquante zone is the largest, ...
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Learning Intention: Students will understand the genetic code as a degenerate triplet code and the steps in gene expression including transcription, RNA processing in eukaryotic cells and translation. Students will also understand gene structure and regulation, especially start/stop, promoter regions, exons/introns and the lac operon.. Transcription - the process by which the DNA code is transcribed into messenger RNA in the nucleus of a cell. (DNA to mRNA ...
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Description de lauteur : Carl et les hommes-boîtes - Autour du Lac Réalisé par Noémie Marsily et Carl Roosens Extrait de lalbum La Paroi de ton Ventre
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Purpose: : To develop a mouse line in which a gene of interest can be removed in keratocytes-specific manner upon induction by doxycycline. Methods: : Two transgenes including Kera3.2-int-rtTA and tet-O-Cre were co-microinjected into the fertilized mouse eggs to create transgenic mice. The Kera3.2-int-rtTA /tet-O-Cre transgenic mice were crossed with reporter Rosa26-LacZ mice to obtain Kera3.2-int-rtTA /tet-O-Cre/Rosa26-LacZ triple transgenic mice. The Cre activity was assessed by the detection of whole mount X-gal staining in corneal stroma of triple transgenic mice after administration of doxycycline via drinking water and chow. Results: : We obtained two independent transgenic founder lines in which the Kera3.2-int-rtTA and tet-O-Cre transgenes were co-segregated at the chromosome level. Administration of doxycycline (Dox) to transgenic Kera3.2-int-rtTA /tet-O-Cre mice did not induce expression of LacZ. The Kera3.2-int-rtTA /tet-O-Cre/Rosa26-LacZ triple transgenic mice showed a little bit ...
We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+ in each strain can occur only by a specific frameshift at these sequences. Monotonous runs of As (or of Ts on the opposite strand) and Gs (or Cs) have been constructed, as has an alternating -C-G- sequence. These specific frameshift indicator strains complement a set of six previously described strains which detect each of the base substitutions. We have examined a variety of mutagens and mutators for their ability to cause reversion to Lac+. Surprisingly, frameshifts are well stimulated at many of these runs by ethyl methanesulfonate, N-methyl-N-nitro-N-nitrosoguanidine and 2-amino-purine, mutagens not widely known to induce frameshifts. A comparison of ethyl methanesulfonate, ...
Background Vaccination against tumour-associated antigens is one approach to elicit anti-tumour responses. We investigated the effect of polynucleotide (DNA) vaccination using a model antigen (E. coli lacZ) in a syngeneic gliosarcoma model (9L). Methods Fisher 344 rats were vaccinated thrice by intramuscular injection of a lacZ-encoding or a control plasmid in weekly intervals. One week after the last vaccination, lacZ-expressing 9L cells were implanted into the striatum. Results After 3 weeks, in lacZ-vaccinated animals the tumours were significantly smaller than in control-vaccinated animals. In cytotoxic T cell assays lysis rates of ,50 % could only be observed in a few of the lacZ-vaccinated animals. This response was directed against lacZ-expressing and parental 9L cells but not against syngeneic MADB 106 adenocarcinoma cells. In Elispot assays interferon-γ production was observed upon stimulation with 9LlacZ and 9L wild-type but not MADB 106 cells. This response was higher for ...
When creating a LACP Link Aggregation, all Force10 switches (with the exception of the I/O Aggregator) have two transmit timer values. This value determines how often the ports send LACPDUs. The default is one LACPDU sent every 1 second. You can also configure it to send one every 30 seconds with the lacp long-timeout command. Long-timeout is best practice and recommended for the Force10 switches.. When the LACPDU transmit timer values mismatch, this can cause intermittent ungrouping of ports, thus causing connection issues. The following command and output helps determine how many LACPDUs being sent and received. This example uses LACP 10.. FTOS#clear lacp 10 counters. FTOS#show lacp 10 counters. ----------------------------------------------------------------------. LACP PDU Marker PDU Unknown Illegal. Port Xmit Recv Xmit Recv Pkts Rx Pkts Rx. ----------------------------------------------------------------------. Gi 0/1 4560 152 0 0 0 0. Gi 0/3 4560 152 0 0 0 0. As you can see, Xmit ...
Top Stories by EmailSUBSCRIBE Noted consumer activist and columnist Jehangir Gai fought this case and helped Deepak Rajmal Kothari to get entire maturity value as mentioned on the policy, loyalty bonus and 9% interest. Mumbai-based Deepak Rajmal Kothari had taken LICs Jeevan Saral Policy (with profits). The policy mentioned the maturity value as Rs25 lakh, death benefit as Rs3,94,900, and accident benefit as Rs15 lakh. For the 11 year term, Mr Kothari paid total premium of Rs13.65 lakh to LIC. However, after maturity LIC offered to pay just Rs3.94 lakh plus Rs1.68 lakh as profit or loyalty addition. When Mr Kothari contested, LIC told him that due to typographical error, the maturity and death benefit values had got interchanged in his Jeevan Saral policy. Thousands of policy holders feel badly cheated by the Jeevan Saral policy and it is an issue that Moneylife Foundation had taken to the Supreme Court. However, consumer courts have proved the better forum because LIC has not succeeded at the ...
Increased mutation rates under stress allow bacterial populations to adapt rapidly to stressors, including antibiotics. Here we evaluate existing models for the evolution of stress-induced mutagenesis and present a new model arguing that it evolves as a result of a complex interplay between direct selection for increased stress tolerance, second-order selection for increased evolvability and genetic drift. Further progress in our understanding of the evolutionary biology of stress and mutagenesis will require a more detailed understanding both of the patterns of stress encountered by bacteria in nature and of the mutations that are produced under stress. © 2013 Macmillan Publishers Limited. All rights reserved.
Spontaneous mutants arise among nondividing populations of Escherichia coli in apparent response to selective conditions. In this report we investigate several hypotheses to account for the role of selection in the production of these directed or adaptive mutations. We found that the Lac+ phenotypes of some mutants that arise late after lactose selection are due to suppressor mutations that are unlinked to the mutant lacZ allele; thus the production of these Lac+ mutants does not require an information flow from successful proteins back to the DNA that encodes them. Transcriptional induction of the lac operon, even in the presence of another, utilizable carbon source, did not stimulate the occurrence of Lac+ mutants in the absence of lactose, indicating that the role of the selective agent is not merely to induce transcription. The absence of two DNA repair pathways-methyl-directed mismatch repair and alkylation repair-also did not result in an accumulation of Lac+ mutants in the absence of ...
University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. ...
Explanation of A & R The function of (3-galactosidase enzyme in lactose metabolism is to form glucose by cleaving lactose. Thus if both glucose and lactose are present in the growth medium, activity of lac operon is not needed, and indeed, no p-galactosidase is formed until virtually all of the glucose in the culture medium is consumed. The lack of synthesis of p-galactosidase is a result of lack of synthesis of lac mRNA. No lac mRNA is made in the presence of glucose, because in addition to an inducer for inactivating the lac i repressor, another element (cAMP-CAP) is needed for initiating lac mRNA synthesis. The activity of this element is regulated by the concentration of glucose ...
After a short introduction to the who/what/why for this class, well start handling the programming material thats at our fingertips...in our fingertips, even! As you work through this DNA isolation protocol below, be mindful of your questions and your own reactions to this activity. Is this the first time youve ever handled a lump of DNA? How hard is it to isolate DNA, both technically speaking and in terms of the needed equipment? What surprised you? What did you learn and what questions do you still have? What could you do next with this material youve isolated? ...
We offer a one-stop solution for all your transgenic mouse embryos needs. From strategy design to TG mouse embryos screening and LacZ staining.
Bourgeois studied the regulation of gene expression using the bacterial lactose (lac) operon as a model system. In the 1960s, when the nature of the regulatory molecule was still unknown, she demonstrated that the lac repressor was a protein. She used that system to carry out the first characterization of the interaction of a regulatory protein with DNA. She later studied the regulation of genes in animal cells and eventually identified compounds that could be useful to reverse multidrug resistance in cancer. More recently, Bourgeois has turned her attention to the history of science-specifically, the early history of the Salk Institute. She is the author of the book, Genesis of the Salk Institute: The Epic of its Founders ...
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These mice have the Ple53-lacZ transgene targeted as a single copy knockin into the upstream region of the |i|Hprt|/i| locus on the X chromosome. The promoter/regulatory regions of the human |i|DCX|/i| gene direct expression of β-galactosidase.
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: Fond du Lac Outlook 2012, Author: Gannett Wisconsin Media, Name: Fond du Lac Outlook 2012, Length: 52 pages, Page: 1, Published: 2012-05-20
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
lac insect İngilizce - Türkçe Sözlük Çevirisini öğrenin. Ek olarak lac insect lak böcek çevirisinin detaylarını görün
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The LAC should be completed by the LHD (not the site) in regards to the CFHL work that has been done this fiscal year. During this time of Shelter-in-Place, you should be able to complete the LAC with information you have and do not need to consult the site. You only need to mark the sections or items on the LAC relevant to your work this fiscal year. (This is different from the SLAQs where all questions should be completed). The LAC is due by end of fiscal year; however if your CFHL activities have been completed for the year (for example, no additional school activities will take place this year), please go ahead and complete the LAC now ...
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Jan 16, 2018 - Entire home/flat for £80. The Tour de Lac apartment block is in the heart of Le Lavachet. It benefits from a great location at the foot of the piste, allowing easy access to...
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Financial creditors, including banks, realised Rs2.45 lakh crore from approved resolution plans for 394 corporate insolvency resolution cases under the Insolvency and Bankruptcy Code (IBC) as on 30th June,
Agnesian HealthCare offers classes and activities in the Fond du Lac area ranging from Walk with a Doc, childbirth, support groups, CPR, yoga and more.
Read more about Rs 18 lakh for 15 days: 7-yr-olds death in Gurgaon hospital sparks debate on Business Standard. Union health ministry to take action against hospital
According to government data, over 28 lakh people were vaccinated against Covid-19 in India on Thursday, of which Uttar Pradesh alone administered more than 3,82,259 doses.
Si vous r vez d un s jour de p che, de chasse, de vacances en famille au c ur de la magnifique for t bor ale, vous serez conquis par le Domaine de la Pourvoirie du lac Dionne.
Ten lakh forty-five thousand beneficiaries are getting maternal and lactating mother allowances from the Women and Child Affairs Ministry. The government
A 14-year-old boy from Vadodara fled to Goa with Rs 1.5 lakh and spent a lot in clubs after his parents scolded him for not studying. He was reunited with his family by cops on December 26.
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Homeopathic Lac Felinum indications, uses & symptoms from 12 cross linked materia medicas. Available 4C-30C, 200C, 8X-30X, 1M-10M
Cooking gas consumers with taxable income of more than Rs.10 lakh per annum will no longer get benefit of the subsidy on gas cylinders, the government announced on Monday
Nearly 2 months after losing jobs due to Covid lockdown reopening of Surat is new hope for lakhs of Odia migrants only if they return
4 people were arrested with heroin worth Rs 3.5 lakh during an operation in south Mizorams Siaha town on Tuesday, an official said
As India tested more than 11.7 lakh samples to detect Covid-19 on Thursday, high testing levels helped keep.. • India • One News Page: Friday, 4 September 2020
retro virus titer - posted in Molecular Biology: can someone tell me what is the best way to find aretrovirus titer when i dont have a lac z plasmid ?
As towards the revised goal of Rs 4.31 lakh crore, thus far this fiscal CGST assortment stands at Rs 1.71 lakh crore. The COVID-19 pandemic has hit Items and Companies Tax (GST) collections, with India thus far this monetary 12 months reaching solely lower than half the revised full-year goal. The Central authorities has collected solely 47 per cent of the revised full-year goal of Rs 6.90 lakh crore GST collections. It could be famous that the annual GST figures within the Price range doc embrace solely the Centres share and the Built-in GST element. It was estimated that out ...
Rs 8.35 lakh per annumThe candidates will get a remuneration of Rs 8.35 lakh per annum.. Graduate Engineers: The candidates will get a pay scale of Rs 8.35 lakh per annum with a grade payment of Rs 40,000 to 1,40,000 per year.. The candidates can apply online through the official website, celindia.co.in on or before April 16, 2019... ...