Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression |...
DNA viruses, such as herpes simplex virus type 1 (HSV-1), Simian virus 40 (SV40), and Cytomegaloviruses (CMV), start their replicative processes and transcription at specific nuclear domains known as ND10 (nuclear domain 10, also called PML bodies). It has been previously determined that for HSV-1 and SV40, a short DNA sequence and its binding protein are required and sufficient for cell localization of viral DNA replication and gene transcription. Our recent observations provide evidence that a foreign (not endogenous) DNA/protein complex in the nucleus recruits ND10 proteins. First, the complexes formed from the bacterial lac operator DNA and its binding protein (lac repressor), or from HPV11 (human papillomavirus 11) origin DNA and its binding protein (E2), co-localized with different ND10 proteins. Second, the HSV-1 amplicon without inserted lac operator DNA repeats distributed in the nucleus randomly, whereas the amplicon with lac operator DNA repeats associated with ND10, suggesting that DNA
User:Dstoebel - OpenWetWare
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution In Press. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. 22:683-690. Full ...
User:Dstoebel - OpenWetWare
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution, 27:2105-2112. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics, 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. ...
Lac Operon
Get all questions and answers of Molecular Basis Of Inheritance lac-operon of neet1 Genetics And Evolution on TopperLearning. TopperLearnings Experts and Students has answered all of Molecular Basis Of Inheritance Lac Operon of Neet1 Genetics And Evolution questions in detail.
Other Cloning Protocols
The first gene in the E. coli lac operon is lacZ, which encodes ß-galactosidase (ß-gal). The active form of ß-gal is a tetramer and hydrolyses lactose into glucose and galactose. Deleting amino acids 11-41 of ß-gal (called the lacZ?M15 mutation) means the enzyme is unable to form a tetramer and is non-functional (Langley et al. 1975). It was discovered that supplying amino acids 1-59 (the a-peptide) of ß-gal in trans (separately) allowed the truncated ß-gal to form tetramers and function again (Ullmann et al. 1967; Langley et al. 1975). Rescuing ß-gal by supplying the a-peptide in this way was termed a-complementation. Later, Vieira and colleagues (Vieira & Messing 1982) realised that a-complementation could be used to screen E. coli colonies for the presence of inserts. They cloned the a-peptide coding region into a pUC plasmid and then introduced a multiple cloning site (MCS) into the middle of that region. When a piece of DNA is ligated into the MCS, it disrupts the a-peptide, ...
Other Cloning Protocols
The first gene in the E. coli lac operon is lacZ, which encodes ß-galactosidase (ß-gal). The active form of ß-gal is a tetramer and hydrolyses lactose into glucose and galactose. Deleting amino acids 11-41 of ß-gal (called the lacZ?M15 mutation) means the enzyme is unable to form a tetramer and is non-functional (Langley et al. 1975). It was discovered that supplying amino acids 1-59 (the a-peptide) of ß-gal in trans (separately) allowed the truncated ß-gal to form tetramers and function again (Ullmann et al. 1967; Langley et al. 1975). Rescuing ß-gal by supplying the a-peptide in this way was termed a-complementation. Later, Vieira and colleagues (Vieira & Messing 1982) realised that a-complementation could be used to screen E. coli colonies for the presence of inserts. They cloned the a-peptide coding region into a pUC plasmid and then introduced a multiple cloning site (MCS) into the middle of that region. When a piece of DNA is ligated into the MCS, it disrupts the a-peptide, ...
What is Positive Regulation of Lac Operon?
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Lac operon
Sorry again for the millions of qns but Im doing quite badly in this subject so I need loads of help! Could you just check my answer for this qn? A lacI mutatio
Lac Operon | Profiles RNS
Friedli M, Barde I, Arcangeli M, Verp S, Quazzola A, Zakany J, Lin-Marq N, Robyr D, Attanasio C, Spitz F, Duboule D, Trono D, Antonarakis SE. A systematic enhancer screen using lentivector transgenesis identifies conserved and non-conserved functional elements at the Olig1 and Olig2 locus. PLoS One. 2010 Dec 29; 5(12):e15741 ...
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AID 672169 - Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing...
BioAssay record AID 672169 submitted by ChEMBL: Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing luciferase, lac Z gene incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by reporter gene assay.
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Cardiomyocytes fused with adult immature somatic cells | Open-i
Cardiomyocytes fused with adult immature somatic cells in vitro. (A) LacZ-expressing cardiomyocytes of neonatal rats were cocultured with GFP+ bone marrow mesen
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Positive and negative regulation, Biology
Biology Assignment Help, Positive and negative regulation, Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 The lac operon is an brilliant example of negative control or negative regulation of gene
13BIS101S2013GeneRegulLect13-1 - The modulation of transcription from the lac lac
View Notes - 13BIS101S2013GeneRegulLect13-1 from BIS 101 at UC Davis. r producing the cyclic bond in ATP). The
reverse happens when glucose is high.
reverse
BIS101-001, Spring 2013-Genes and Gene
2015 Estrategia Regional para la Red de Políticas Públicas de Latinoamérica para la Producción Limpia de Ladrillos (PAN LAC)...
2015_Estrategia Regional para la Red de Políticas Públicas de Latinoamérica para la Producción Limpia de Ladrillos (PAN LAC)_CCAC_CHRE.pdf ...
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GeneCorner plasmid details pRS425
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide ...
GeneCorner plasmid details pRS414
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide ...
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Product Showcase
Arinna has a significant presence in the CNS specialty therapeutic segment. Arinna has launched over 42 brands with more than 140 SKUs providing ray of hope to more than 3Lacs patients ...
Mutagenesis induced by oral carcinogens in lacZ mouse (Muta™Mouse) tongue and other oral tissues : Carcinogenesis - oi
Animal models for carcinogenesis of the oral cavity are limited, although this disease is often fatal or disfiguring and its incidence in the USA is ~30 000 cases/year. Short-term whole-animal models for this disease should prove valuable in the investigation of factors affecting oral carcinogenesis. In this study we observed that a group of oral carcinogens are clearly mutagenic in the lacZ transgenic mouse oral cavity. The carcinogens 4-nitroquinoline-N-oxide (4-NQO), benzo[a]pyrene (B[a]P), N-nitroso-N-methylurea (NMU), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), nitrosonornicotine (NNN) and 7,12-dimethylbenzanthracene (DMBA) were all mutagenic in a mixture of pooled oral tissues (gingival, buccal, pharyngeal and sublingual) and in the tongue. All agents except DMBA (which was swabbed in the oral cavity) and B[a]P (by gavage) were given in drinking water for 2-4 weeks followed by a 2 week expression period before killing. With one exception, groups of 4-5 female mice were treated. ...
Sandwalk: Repression of the lac Operon
lac repressor binds simultaneously to two sites near the promoter of the lac operon. Repressor-binding sites are called operators. One operator (O1) is adjacent to the promoter, and the other (O2) is within the coding region of lacZ. When bound to both operators, the repressor causes the DNA to form a stable loop that can be seen in electron micrographs of the complex formed between lac repressor and DNA (bottom figure). The interaction of lac repressor with the operator sequences may block transcription by preventing the binding of RNA polymerase to the lac promoter. However, it is now known that, in some cases, both lac repressor and RNA polymerase can bind to the promoter at the same time. Thus, the repressor may also block transcription initiation by preventing formation of the open complex and promoter clearance. A schematic diagram of lac repressor bound to DNA in the presence of RNA polymerase is shown in the figure on the right. [See Mondays Molecule #61 for another view.] The diagram ...
PPT - L1 The lac operon L2 The trp operon L3 Transcriptional regulation by PowerPoint Presentation - ID:6150074
Section L:Regulation of transcription in prokaryotes. Molecular Biology. L1 The lac operon L2 The trp operon L3 Transcriptional regulation by alternative σ factors. Molecular Biology. L1:The LAC Operon. Operon - what is it? Slideshow 6150074 by otto-barker
Click to see a list of this datasets variables and the complete list of metadata attributes.
A2018016122500.L2_LAC_OC.nc, A2018016123000.L2_LAC_OC.nc, A2018016134000.L2_LAC_OC.nc, A2018016134500.L2_LAC_OC.nc, A2018016135000.L2_LAC_OC.nc, A2018016135500.L2_LAC_OC.nc, A2018016140000.L2_LAC_OC.nc, A2018016152000.L2_LAC_OC.nc, A2018016153000.L2_LAC_OC.nc, A2018016153500.L2_LAC_OC.nc, A2018016154000.L2_LAC_OC.nc, A2018016154500.L2_LAC_OC.nc, A2018016170000.L2_LAC_OC.nc, A2018016170500.L2_LAC_OC.nc, A2018016171000.L2_LAC_OC.nc, A2018016171500.L2_LAC_OC.nc, A2018016172000.L2_LAC_OC.nc, A2018016172500.L2_LAC_OC.nc, A2018016183500.L2_LAC_OC.nc, A2018016184000.L2_LAC_OC.nc, A2018016184500.L2_LAC_OC.nc, A2018016185000.L2_LAC_OC.nc, A2018016185500.L2_LAC_OC.nc, A2018016190000.L2_LAC_OC.nc, A2018016190500.L2_LAC_OC.nc, A2018016201500.L2_LAC_OC.nc, A2018016202000.L2_LAC_OC.nc, A2018016202500.L2_LAC_OC.nc, A2018016203000.L2_LAC_OC.nc, A2018016204000.L2_LAC_OC.nc, A2018016215500.L2_LAC_OC.nc, A2018016220000.L2_LAC_OC.nc, A2018016220500.L2_LAC_OC.nc, A2018016221000.L2_LAC_OC.nc, ...
Mutation frequency and type during ageing in mouse seminiferous tubules<...
TY - JOUR. T1 - Mutation frequency and type during ageing in mouse seminiferous tubules. AU - Martin, Sandra L.. AU - Hopkins, Craig L.. AU - Naumer, Anne. AU - Dollé, Martijn E T. AU - Vijg, Jan. PY - 2001. Y1 - 2001. N2 - Mutations arise in the germline by errors of replication, recombination and repair, and the movement of transposable elements. Transgenic mice bearing reporter genes such as lacZ have proven useful for measurements of spontaneous and induced mutation frequencies, as well as studies of the effects of ageing. In this study, testicular DNA from lacZ transgenic mice was examined for age-related effects on mutation frequency and type. The recovered transgene was tested for simple substitutions and rearrangements including transposition of endogenous mobile elements. There was no evidence for either an age-related accumulation of mutations, or for the insertion of retrotransposons into the lacZ reporter gene in the testis. We conclude that the frequency of retrotransposition of ...
Antigen gene transfer to cultured human dendritic cells using recombinant avipoxvirus vectors. - The Christie Research...
Advances in understanding the role of dendritic cells (DCs) as the major antigen (Ag)-presenting cell type of the immune system combined with the recent development of methods for the ex vivo expansion of human DCs have opened the possibility for the transfer of tumor Ags to DCs with a view toward tumor immunotherapy. In this study, we examined the feasibility of Ag transfer to cultured human DCs using the host range-restricted avipoxvirus, fowlpoxvirus (FWPV). FWPV was found to infect and express a lacZ marker gene in a number of mammalian cell lines of fibroblastic, epithelial, and hemopoietic lineage origins. LacZ recombinant FWPV (rFWPV) was found subsequently to infect human DCs that had been cultured ex vivo from peripheral blood monocytes. Using rFWPV containing lacZ under the control of a vaccinia virus (VV) early/late promoter (p7.5K) and a 10 plaque-forming units per cell multiplicity of infection, ,80% of cells expressed the lacZ marker gene. Quantitative analysis showed that the ...
Microsoft word - tepper.doc
Having demonstrated that PEMF has a potent effect on endothelial cells in vitro, we examined whether PEMF was able to stimulate angiogenesis in vivo. Matrigel is a soluble basement membrane preparation, and when implanted s.c. supports vascular ingrowth. Matrigel was injected s.c. into tie2/lacZ transgenic mice that were housed in cages emitting PEMF for 8 h a day or control cages. After 3, 10, and 14 days, there was significantly greater vascular ingrowth into the matrix in PEMF-treated animals, confirmed by staining specific for endothelial markers CD31 and Tie-2. PEMF increased the vascular ingrowth more than twofold by day 3 (13.3±0.41 vs. 5.8±0.28 cells/hpf; P,0.01). This increase in vascular ingrowth persisted through days 10 and 14 (16.6±0.49 vs 12.6±0.43 cells/hpf; P,0.01, and 19.4±0.55 vs. 14.8±0.40 cells/hpf; P,0.01, respectivelLISA confirmed a twofold increase in FGF-2 in PEMF-treated matrigel, but demfactors TPO, Ang-2, and EGF (data not shown). In this study, we demonstrate ...
EMBOSS: chaos
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT The nucleotide sequence of the lac operator; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
EMBOSS: supermatcher
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT The nucleotide sequence of the lac operator; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
Improved single and multicopy lac-based cloning vectors for protein and operon fusions
We describe several new vectors for the construction of operon and protein fusions to the Escherichia coli lacZ gene. In vitro constructions utilize multicopy plasmids containing suitable cloning sites located between upstream transcription terminators and downstream lac operon segments whose lacZ g …
Expression of the nlsLacz gene in dendritic cells derived from retrovirally transduced peripheral blood CD34+ cells |...
BACKGROUND AND OBJECTIVE: Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS: To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ, a marker gene used here as a model, in the presence of IL-3, IL-6, GM-CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM-CSF and TNF-a. RESULTS: Addition of IL-4 was found to induce dendritic differentiation preferentially by inhibiting proliferation and differentiation of the macrophage lineage. As assessed by X-Gal staining, LacZ gene expression was observed in cells from both the dendritic lineage (CD1a+/CD14-) which still exhibits the highest immunostimulatory activity in mixed ...
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Secondary Sugars(metabolism/sugar/bacteria/Lac Operon) - Biology-Online
Not sure why I cant edit the above part, but I stumbled across something that seemed to be of use; that secondary sugars might be sugars the require cAMP and CRP to be the effector of the operon(along with the operon inducer of course). Since gallactose is what the cell wants the most then the other metabolic operons(like lactose) should be off so any sugar that isnt gallactose is secondary ...
lactose operon
Lac Operon - Revision Cards in A Level and IB Biology
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Kivalliq Announces 60% Increase in Uranium Resource; Lac 50 Trend Deposits Expand to 43.3 Million lbs U3O8 | SYS-CON CANADA
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translation
Learning Intention: Students will understand the genetic code as a degenerate triplet code and the steps in gene expression including transcription, RNA processing in eukaryotic cells and translation. Students will also understand gene structure and regulation, especially start/stop, promoter regions, exons/introns and the lac operon.. Transcription - the process by which the DNA code is transcribed into messenger RNA in the nucleus of a cell. (DNA to mRNA ...
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COVID-19 impact: Content creation has been tricky but influencers are thinking out of the box
For a million views, a YouTuber can charge Rs 5-6 lakh while another may charge Rs 15-20 lakh. Thats because of the kind of depth they have with the audience
The Gorge of Orbe - Yverdon-les-Bains Region Jura Lac (Switzerland)
Between Vallorbe and Orbe, this route of 17 km invites the hiker to the heart of a rich and diversified natural environment for a 4h and 20mn walk.
Billava B.com. Grooms - Find lakhs of Billava B.com. Grooms - Bharatmatrimony.com
Looking for Billava B.com. Grooms? Find your perfect Billava B.com. Grooms, Boys for Matrimony on BharatMatrimony - the Most Trusted Brand. Register free to find serious like-minded Billava B.com. Grooms looking for Matrimony.
Dirty dozen and clean 15 | Kira Baum ND LAC
The Environmental Working Group has released an updated list of pesticides in produce. Check here for the clean 15 and dirty dozen for a good guide on which foods to buy organic http://www.ewg.org/foodnews/summary/
India Reports 1 Lakh New COVID-19 Cases, Lowest In Two Months - Breaking News Today
India has been reporting positivity rate below 10 per cent for 14 consecutive days now.New Delhi: Indias Covid chart continued to improve today with the
PM-KMY Provides Rs. 36000 per Year; Over 21.40 Lakh Farmers Enrolled
As on 23rd July 2021, a total of 21, 40, 262 farmers have joined Pradhan Mantri Kisan Maan-Dhan Yojana. The scheme provides Rs. 36000 per year.
Maruti Ertiga Limited Edition launched in India
Maruti Suzuki has launched the Maruti Ertiga Limited Edition, with prices starting at INR 7.85 lakh. It features unique styling and additional equipment.