DNA viruses, such as herpes simplex virus type 1 (HSV-1), Simian virus 40 (SV40), and Cytomegaloviruses (CMV), start their replicative processes and transcription at specific nuclear domains known as ND10 (nuclear domain 10, also called PML bodies). It has been previously determined that for HSV-1 and SV40, a short DNA sequence and its binding protein are required and sufficient for cell localization of viral DNA replication and gene transcription. Our recent observations provide evidence that a foreign (not endogenous) DNA/protein complex in the nucleus recruits ND10 proteins. First, the complexes formed from the bacterial lac operator DNA and its binding protein (lac repressor), or from HPV11 (human papillomavirus 11) origin DNA and its binding protein (E2), co-localized with different ND10 proteins. Second, the HSV-1 amplicon without inserted lac operator DNA repeats distributed in the nucleus randomly, whereas the amplicon with lac operator DNA repeats associated with ND10, suggesting that DNA
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution In Press. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. 22:683-690. Full ...
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution, 27:2105-2112. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics, 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. ...
Get all questions and answers of Molecular Basis Of Inheritance lac-operon of neet1 Genetics And Evolution on TopperLearning. TopperLearnings Experts and Students has answered all of Molecular Basis Of Inheritance Lac Operon of Neet1 Genetics And Evolution questions in detail.
The first gene in the E. coli lac operon is lacZ, which encodes ß-galactosidase (ß-gal). The active form of ß-gal is a tetramer and hydrolyses lactose into glucose and galactose. Deleting amino acids 11-41 of ß-gal (called the lacZ?M15 mutation) means the enzyme is unable to form a tetramer and is non-functional (Langley et al. 1975). It was discovered that supplying amino acids 1-59 (the a-peptide) of ß-gal in trans (separately) allowed the truncated ß-gal to form tetramers and function again (Ullmann et al. 1967; Langley et al. 1975). Rescuing ß-gal by supplying the a-peptide in this way was termed a-complementation. Later, Vieira and colleagues (Vieira & Messing 1982) realised that a-complementation could be used to screen E. coli colonies for the presence of inserts. They cloned the a-peptide coding region into a pUC plasmid and then introduced a multiple cloning site (MCS) into the middle of that region. When a piece of DNA is ligated into the MCS, it disrupts the a-peptide, ...
The first gene in the E. coli lac operon is lacZ, which encodes ß-galactosidase (ß-gal). The active form of ß-gal is a tetramer and hydrolyses lactose into glucose and galactose. Deleting amino acids 11-41 of ß-gal (called the lacZ?M15 mutation) means the enzyme is unable to form a tetramer and is non-functional (Langley et al. 1975). It was discovered that supplying amino acids 1-59 (the a-peptide) of ß-gal in trans (separately) allowed the truncated ß-gal to form tetramers and function again (Ullmann et al. 1967; Langley et al. 1975). Rescuing ß-gal by supplying the a-peptide in this way was termed a-complementation. Later, Vieira and colleagues (Vieira & Messing 1982) realised that a-complementation could be used to screen E. coli colonies for the presence of inserts. They cloned the a-peptide coding region into a pUC plasmid and then introduced a multiple cloning site (MCS) into the middle of that region. When a piece of DNA is ligated into the MCS, it disrupts the a-peptide, ...
When both glucose and lactose are present in the medium, the transcription of the genes z, y and a are inhibited. This phenomenon is called catabolite repression or glucose effect. The effect of
Sorry again for the millions of qns but Im doing quite badly in this subject so I need loads of help! Could you just check my answer for this qn? A lacI mutatio
BioAssay record AID 672169 submitted by ChEMBL: Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing luciferase, lac Z gene incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by reporter gene assay.
Cardiomyocytes fused with adult immature somatic cells in vitro. (A) LacZ-expressing cardiomyocytes of neonatal rats were cocultured with GFP+ bone marrow mesen
Biology Assignment Help, Positive and negative regulation, Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 The lac operon is an brilliant example of negative control or negative regulation of gene
View Notes - 13BIS101S2013GeneRegulLect13-1 from BIS 101 at UC Davis. r producing the cyclic bond in ATP). The reverse happens when glucose is high. reverse BIS101-001, Spring 2013-Genes and Gene
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Animal models for carcinogenesis of the oral cavity are limited, although this disease is often fatal or disfiguring and its incidence in the USA is ~30 000 cases/year. Short-term whole-animal models for this disease should prove valuable in the investigation of factors affecting oral carcinogenesis. In this study we observed that a group of oral carcinogens are clearly mutagenic in the lacZ transgenic mouse oral cavity. The carcinogens 4-nitroquinoline-N-oxide (4-NQO), benzo[a]pyrene (B[a]P), N-nitroso-N-methylurea (NMU), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), nitrosonornicotine (NNN) and 7,12-dimethylbenzanthracene (DMBA) were all mutagenic in a mixture of pooled oral tissues (gingival, buccal, pharyngeal and sublingual) and in the tongue. All agents except DMBA (which was swabbed in the oral cavity) and B[a]P (by gavage) were given in drinking water for 2-4 weeks followed by a 2 week expression period before killing. With one exception, groups of 4-5 female mice were treated. ...
lac repressor binds simultaneously to two sites near the promoter of the lac operon. Repressor-binding sites are called operators. One operator (O1) is adjacent to the promoter, and the other (O2) is within the coding region of lacZ. When bound to both operators, the repressor causes the DNA to form a stable loop that can be seen in electron micrographs of the complex formed between lac repressor and DNA (bottom figure). The interaction of lac repressor with the operator sequences may block transcription by preventing the binding of RNA polymerase to the lac promoter. However, it is now known that, in some cases, both lac repressor and RNA polymerase can bind to the promoter at the same time. Thus, the repressor may also block transcription initiation by preventing formation of the open complex and promoter clearance. A schematic diagram of lac repressor bound to DNA in the presence of RNA polymerase is shown in the figure on the right. [See Mondays Molecule #61 for another view.] The diagram ...
Section L:Regulation of transcription in prokaryotes. Molecular Biology. L1 The lac operon L2 The trp operon L3 Transcriptional regulation by alternative σ factors. Molecular Biology. L1:The LAC Operon. Operon - what is it? Slideshow 6150074 by otto-barker
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TY - JOUR. T1 - Mutation frequency and type during ageing in mouse seminiferous tubules. AU - Martin, Sandra L.. AU - Hopkins, Craig L.. AU - Naumer, Anne. AU - Dollé, Martijn E T. AU - Vijg, Jan. PY - 2001. Y1 - 2001. N2 - Mutations arise in the germline by errors of replication, recombination and repair, and the movement of transposable elements. Transgenic mice bearing reporter genes such as lacZ have proven useful for measurements of spontaneous and induced mutation frequencies, as well as studies of the effects of ageing. In this study, testicular DNA from lacZ transgenic mice was examined for age-related effects on mutation frequency and type. The recovered transgene was tested for simple substitutions and rearrangements including transposition of endogenous mobile elements. There was no evidence for either an age-related accumulation of mutations, or for the insertion of retrotransposons into the lacZ reporter gene in the testis. We conclude that the frequency of retrotransposition of ...
Advances in understanding the role of dendritic cells (DCs) as the major antigen (Ag)-presenting cell type of the immune system combined with the recent development of methods for the ex vivo expansion of human DCs have opened the possibility for the transfer of tumor Ags to DCs with a view toward tumor immunotherapy. In this study, we examined the feasibility of Ag transfer to cultured human DCs using the host range-restricted avipoxvirus, fowlpoxvirus (FWPV). FWPV was found to infect and express a lacZ marker gene in a number of mammalian cell lines of fibroblastic, epithelial, and hemopoietic lineage origins. LacZ recombinant FWPV (rFWPV) was found subsequently to infect human DCs that had been cultured ex vivo from peripheral blood monocytes. Using rFWPV containing lacZ under the control of a vaccinia virus (VV) early/late promoter (p7.5K) and a 10 plaque-forming units per cell multiplicity of infection, ,80% of cells expressed the lacZ marker gene. Quantitative analysis showed that the ...
Having demonstrated that PEMF has a potent effect on endothelial cells in vitro, we examined whether PEMF was able to stimulate angiogenesis in vivo. Matrigel is a soluble basement membrane preparation, and when implanted s.c. supports vascular ingrowth. Matrigel was injected s.c. into tie2/lacZ transgenic mice that were housed in cages emitting PEMF for 8 h a day or control cages. After 3, 10, and 14 days, there was significantly greater vascular ingrowth into the matrix in PEMF-treated animals, confirmed by staining specific for endothelial markers CD31 and Tie-2. PEMF increased the vascular ingrowth more than twofold by day 3 (13.3±0.41 vs. 5.8±0.28 cells/hpf; P,0.01). This increase in vascular ingrowth persisted through days 10 and 14 (16.6±0.49 vs 12.6±0.43 cells/hpf; P,0.01, and 19.4±0.55 vs. 14.8±0.40 cells/hpf; P,0.01, respectivelLISA confirmed a twofold increase in FGF-2 in PEMF-treated matrigel, but demfactors TPO, Ang-2, and EGF (data not shown). In this study, we demonstrate ...
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT "The nucleotide sequence of the lac operator"; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT "The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
ID J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP. XX AC J01636; J01637; K01483; K01793; XX DT 30-NOV-1990 (Rel. 26, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 8) XX DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes. XX KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene; KW lacZ gene; mutagenesis; palindrome; promoter region; KW thiogalactoside acetyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1243-1266 RX DOI; 10.1073/pnas.70.12.3581. RX PUBMED; 4587255. RA Gilbert W., Maxam A.; RT "The nucleotide sequence of the lac operator"; RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973). XX RN [2] RP 1246-1308 RX DOI; 10.1073/pnas.70.12.3585. RX PUBMED; 4587256. RA Maizels N.M.; RT "The nucleotide sequence of the lactose messenger ribonucleic acid RT transcribed ...
BACKGROUND AND OBJECTIVE: Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS: To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ, a marker gene used here as a model, in the presence of IL-3, IL-6, GM-CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM-CSF and TNF-a. RESULTS: Addition of IL-4 was found to induce dendritic differentiation preferentially by inhibiting proliferation and differentiation of the macrophage lineage. As assessed by X-Gal staining, LacZ gene expression was observed in cells from both the dendritic lineage (CD1a+/CD14-) which still exhibits the highest immunostimulatory activity in mixed ...
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Not sure why I cant edit the above part, but I stumbled across something that seemed to be of use; that secondary sugars might be sugars the require cAMP and CRP to be the effector of the operon(along with the operon inducer of course). Since gallactose is what the cell wants the most then the other metabolic operons(like lactose) should be off so any sugar that isnt gallactose is secondary ...
Group of adjacent and coordinately controlled genes concerned with the metabolism of lactose in E. coli. The lac operon was the first example of a group of genes under the control of an operator region to which a lactose repressor binds. When the
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The geologic characteristics for the Lac Cinquante, J4 and Ray deposits are very similar. Separated by only 1,800 metres and exhibiting the same general trends, it is likely that these three deposits represent the same, or related mineralized structures. Deposits in the Lac 50 Trend are basement hosted, vein-hydrothermal type. The emplacement of uranium is structurally controlled, often associated with a graphite-chlorite tuffaceous metasediment interlayered in Archean metavolcanics. Mineralization consists of disseminated and patchy pitchblende with sulphides within fracture controlled, brecciated, hematite-quartz-carbonate veins that can occur in either the host shear or tuff unit. Potential also exists for unconformity-style uranium deposits to occur on the Angilak Property. The Lac Cinquante deposit is comprised of three distinct mineralized sections occurring over 3.8 kilometres of strike length and interpreted to be off-set by faults. The central "Main" Lac Cinquante zone is the largest, ...
Learning Intention: Students will understand the genetic code as a degenerate triplet code and the steps in gene expression including transcription, RNA processing in eukaryotic cells and translation. Students will also understand gene structure and regulation, especially start/stop, promoter regions, exons/introns and the lac operon.. Transcription - the process by which the DNA code is transcribed into messenger RNA in the nucleus of a cell. (DNA to mRNA ...
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Description de lauteur : Carl et les hommes-boîtes - Autour du Lac Réalisé par Noémie Marsily et Carl Roosens Extrait de lalbum La Paroi de ton Ventre
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Purpose: : To develop a mouse line in which a gene of interest can be removed in keratocytes-specific manner upon induction by doxycycline. Methods: : Two transgenes including Kera3.2-int-rtTA and tet-O-Cre were co-microinjected into the fertilized mouse eggs to create transgenic mice. The Kera3.2-int-rtTA /tet-O-Cre transgenic mice were crossed with reporter Rosa26-LacZ mice to obtain Kera3.2-int-rtTA /tet-O-Cre/Rosa26-LacZ triple transgenic mice. The Cre activity was assessed by the detection of whole mount X-gal staining in corneal stroma of triple transgenic mice after administration of doxycycline via drinking water and chow. Results: : We obtained two independent transgenic founder lines in which the Kera3.2-int-rtTA and tet-O-Cre transgenes were co-segregated at the chromosome level. Administration of doxycycline (Dox) to transgenic Kera3.2-int-rtTA /tet-O-Cre mice did not induce expression of LacZ. The Kera3.2-int-rtTA /tet-O-Cre/Rosa26-LacZ triple transgenic mice showed a little bit ...
We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+ in each strain can occur only by a specific frameshift at these sequences. Monotonous runs of As (or of Ts on the opposite strand) and Gs (or Cs) have been constructed, as has an alternating -C-G- sequence. These specific frameshift indicator strains complement a set of six previously described strains which detect each of the base substitutions. We have examined a variety of mutagens and mutators for their ability to cause reversion to Lac+. Surprisingly, frameshifts are well stimulated at many of these runs by ethyl methanesulfonate, N-methyl-N-nitro-N-nitrosoguanidine and 2-amino-purine, mutagens not widely known to induce frameshifts. A comparison of ethyl methanesulfonate, ...
When creating a LACP Link Aggregation, all Force10 switches (with the exception of the I/O Aggregator) have two transmit timer values. This value determines how often the ports send LACPDUs. The default is one LACPDU sent every 1 second. You can also configure it to send one every 30 seconds with the lacp long-timeout command. Long-timeout is best practice and recommended for the Force10 switches.. When the LACPDU transmit timer values mismatch, this can cause intermittent ungrouping of ports, thus causing connection issues. The following command and output helps determine how many LACPDUs being sent and received. This example uses LACP 10.. FTOS#clear lacp 10 counters. FTOS#show lacp 10 counters. ----------------------------------------------------------------------. LACP PDU Marker PDU Unknown Illegal. Port Xmit Recv Xmit Recv Pkts Rx Pkts Rx. ----------------------------------------------------------------------. Gi 0/1 4560 152 0 0 0 0. Gi 0/3 4560 152 0 0 0 0. As you can see, Xmit ...
Spontaneous mutants arise among nondividing populations of Escherichia coli in apparent response to selective conditions. In this report we investigate several hypotheses to account for the role of selection in the production of these "directed" or "adaptive" mutations. We found that the Lac+ phenotypes of some mutants that arise late after lactose selection are due to suppressor mutations that are unlinked to the mutant lacZ allele; thus the production of these Lac+ mutants does not require an information flow from successful proteins back to the DNA that encodes them. Transcriptional induction of the lac operon, even in the presence of another, utilizable carbon source, did not stimulate the occurrence of Lac+ mutants in the absence of lactose, indicating that the role of the selective agent is not merely to induce transcription. The absence of two DNA repair pathways-methyl-directed mismatch repair and alkylation repair-also did not result in an accumulation of Lac+ mutants in the absence of ...
Explanation of A & R The function of (3-galactosidase enzyme in lactose metabolism is to form glucose by cleaving lactose. Thus if both glucose and lactose are present in the growth medium, activity of lac operon is not needed, and indeed, no p-galactosidase is formed until virtually all of the glucose in the culture medium is consumed. The lack of synthesis of p-galactosidase is a result of lack of synthesis of lac mRNA. No lac mRNA is made in the presence of glucose, because in addition to an inducer for inactivating the lac i repressor, another element (cAMP-CAP) is needed for initiating lac mRNA synthesis. The activity of this element is regulated by the concentration of glucose ...
Bourgeois studied the regulation of gene expression using the bacterial lactose (lac) operon as a model system. In the 1960s, when the nature of the regulatory molecule was still unknown, she demonstrated that the lac "repressor" was a protein. She used that system to carry out the first characterization of the interaction of a regulatory protein with DNA. She later studied the regulation of genes in animal cells and eventually identified compounds that could be useful to reverse multidrug resistance in cancer. More recently, Bourgeois has turned her attention to the history of science-specifically, the early history of the Salk Institute. She is the author of the book, Genesis of the Salk Institute: The Epic of its Founders ...
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: Fond du Lac Outlook 2012, Author: Gannett Wisconsin Media, Name: Fond du Lac Outlook 2012, Length: 52 pages, Page: 1, Published: 2012-05-20
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
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