TY - JOUR. T1 - Cellular mechanisms of growth inhibition of human endometrial cancer cell line by an antagonist of growth hormone-releasing hormone. AU - Zhao, Lin. AU - Yano, Tetsu. AU - Osuga, Yutaka. AU - Nakagawa, Shunsuke. AU - Oishi, Hajime. AU - Wada-Hiraike, Osamu. AU - Tang, Xiaohui. AU - Yano, Naomi. AU - Kugu, Koji. AU - Schally, Andrew V.. AU - Taketani, Yuji. PY - 2008/3. Y1 - 2008/3. N2 - The expression of growth hormone-releasing hormone (GHRH) and its receptors has been demonstrated in peripheral tissues as well as CNS. Recently, the functional splice variant SV1 of GHRH receptor was identified in various human cancers and cancer cell lines. Although antineoplastic activity of GHRH antagonists has been clearly demonstrated, the mechanism of action is incompletely understood. The objective of this study was the investigation of direct anti-proliferative effect of GHRH antagonist MZ-5-156 on HEC-1A human endometrial cancer cell line and the elucidation of underlying mechanisms. ...

TY - JOUR. T1 - Differential mRNA expression of prostaglandin receptor subtypes in macrophage activation. AU - Hubbard, Neil. AU - Lee, S. H.. AU - Lim, D.. AU - Erickson, Kent L. PY - 2001. Y1 - 2001. N2 - Assessing the regulation of macrophage receptors for prostaglandin (PGE2) is essential to understanding the control which that potent lipid mediator has in modulating macrophage activities. The purpose of this study was to assess the differential mRNA expression of PGE2 receptor subtypes (EP) during macrophage exposure to activating and transducing agents. RAW 264.7 macrophages constitutively expressed mRNA for EP2, EP3 and EP4 receptor subtypes. Messenger RNA for EP4 was expressed at a much higher level when compared to EP2 in unstimulated macrophages as assessed by kinetic quantitative RT-PCR. When macrophages were stimulated with LPS, EP2 mRNA levels were 12-fold higher when compared to unstimulated macrophages, while EP4 mRNA remained unchanged. Conversely, mRNA levels of both EP2 and EP4 ...

Pathology Department, Harvard Medical School, Boston, Massachusetts 02115-5701, USA. We have investigated the functional integrity of the retinoblastoma tumor suppressor pathway in five human squamous cell carcinoma lines. Elevated activity of cyclin-dependent kinase 6 (cdk6), a pRB kinase, was detected in all five squamous cell carcinoma lines. Overexpression of the cdk6 protein was detected in one of the five cell lines. The cdk6-specific inhibitor p18ink4C is expressed and associated with cdk6 in all five squamous cell carcinoma lines. In contrast, only very low levels of p16ink4A were detected in these cell lines. This may contribute to the elevated activity of cdk6 in these lines. Elevated activity of cdk6 may result in hyperphosphorylation of the retinoblastoma protein and, therefore, compromise its negative growth-regulatory activity ...

TY - JOUR. T1 - Alpha particle mutagenesis of human lymphoblastoid cell lines. AU - Amundson, S. A.. AU - Chen, D. J.. AU - Okinaka, R. T.. PY - 1996. Y1 - 1996. N2 - Despite being derived from the same donor, the human lymphoblastoid cell lines WTK1 and TK6 have markedly different responses to low LET radiation. We originally observed that WTK1 was more resistant to the cytotoxic effects of X-irradiation, but significantly more sensitive to mutation induction at both the TK and HPRT loci. In an effort to better understand these properties, we have examined the effects of α-particles on these cells. Relative to TK6, WTK1 has enhanced survival and mutation after both X-ray and α-particle exposure. While the HPRT locus was significantly more mutable in WTK1 as a function of α-particle versus X-ray dose, the TK locus was only slightly more sensitive to a-particle mutagenesis. In addition, the slowly growing TK mutants that constitute the majority of X-ray-induced TK mutants of TK6 were recovered ...

TY - JOUR. T1 - Arsenite exposure in human lymphoblastoid cell lines induces autophagy and coordinated induction of lysosomal genes. AU - Bolt, Alicia M.. AU - Douglas, Randi M.. AU - Klimecki, Walter T.. PY - 2010/11/30. Y1 - 2010/11/30. N2 - Chronic exposure to inorganic arsenic is associated with diverse, complex diseases, making the identification of the mechanism underlying arsenic-induced toxicity a challenge. An increasing body of literature from epidemiological and in vitro studies has demonstrated that arsenic is an immunotoxicant, but the mechanism driving arsenic-induced immunotoxicity is not well established. We have previously demonstrated that in human lymphoblastoid cell lines (LCLs), arsenic-induced cell death is strongly associated with the induction of autophagy. In this study we utilized genome-wide gene expression analysis and functional assays to characterize arsenic-induced effects in seven LCLs that were exposed to an environmentally relevant, minimally cytotoxic, ...

Cell Line,cell type,cell line specific transfection reagents,EZ Biosystems is a worldwide provider of transfection and gene expression product and services. Simpler, Faster, and Easier are EZ Biosystems' promises to scientists in the life science research community. One of the major focuses of EZ Biosystems has been the research, development, and manufacture of next generation transfection reagents ---- Avalanche Transfection Reagent series by applying combinatorial chemistry, molecular biology, and cell biology expertise. This series includes: Avalanche-Cell Type/Cell Line-specific Transfection Reagent series: The pre-optimized transfection Reagent series that ensures the highest transfection efficiency and viability for over 100 cell types/cell lines. Avalanche-Omni Transfection Reagent: Broadest spectrum in vitro gene and siRNA transfection with exceptional transfection efficiency and viability. Avalanche-in vivo Transfection Reagent: Extremely powerful for gene functional studies and RNA

Human muscle cell line - human body diagram at muscles. Human Muscle Cell Line encouraged for you to our website, on this period I am going to teach you with regards to Human muscle cell line.. Now, this can be a very first image, human muscle cell line, human muscle cell line atcc, human smooth muscle cell line, human skeletal muscle cell line atcc, human cardiac muscle cell line, human heart muscle cell line, human vascular smooth muscle cell line, human airway smooth muscle cell line, human skeletal muscle myoblast cell line, immortalized human skeletal muscle cell line :. ...

TY - JOUR. T1 - Monoclonal antibody to a nucleolar antigen of human B-lymphoblastoid cells. AU - Todorov, I. T.. AU - Philipova, R. N.. AU - Zhelev, N. Z.. AU - Hadjiolov, A. A.. PY - 1987/3. Y1 - 1987/3. N2 - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of rRNA transcription with Actinomycin D caused redistribution of the 41 5.6 antigen, but even at long term drug action it remains associated with the nucleolar remnants.. AB - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of ...

Zhao, Q., Huo, X., Sun, F., Dong, R.'[Retracted] Polyphenol‑rich extract of ,em,Saliva chinensis,/em, exhibits anticancer activity in different cancer cell lines, and induces cell cycle arrest at the G0/G1‑phase, apoptosis and loss of mitochondrial membrane potential in pancreatic cancer cells'. Molecular Medicine Reports 23, no. 6 (2021): 462. https://doi.org/10.3892/mmr. ...

0361] Cell Line Specific Transfection Reagents/Kits include, e.g., GenJet® (Ver. II) DNA In Vitro Transfection Reagent for 3LL Cell, TransIT®-3T3 Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for NIH3T3 Cell, Human B Cell Nucleofector® Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for B16-F10 Cells, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for BHK-21 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Ca Ski Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Caco-2 Cell, DG44 Transfection Kit, TransIT®-CHO Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for CHO Cell, TransIT®-COS Transfection Kit, TransPass® COS Transfection Reagent, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for COS Cell, Targefect-COS, GenJet® (Ver. II) DNA In Vitro Transfection Reagent ...

Cisplatin is widely used for chemotherapy of head and neck squamous cell carcinoma. However, details of the molecular mechanism responsible for cisplatin resistance are still unclear. The aim of this study was to identify the expression of genes related to cisplatin resistance in oral squamous cell carcinoma cells. A cisplatin-resistant cell line, Tca/cisplatin, was established from a cisplatin-sensitive cell line, Tca8113, which was derived from moderately-differentiated tongue squamous cell carcinoma. Global gene expression in this resistant cell line and its sensitive parent cell line was analyzed using Affymetrix HG-U95Av2 microarrays. Candidate genes involved in DNA repair, the MAP pathway and cell cycle regulation were chosen to validate the microarray analysis results. Cell cycle distribution and apoptosis following cisplatin exposure were also investigated. Cisplatin resistance in Tca/cisplatin cells was stable for two years in cisplatin-free culture medium. The IC50 for cisplatin in Tca

TY - JOUR. T1 - Unusual roles of caspase-8 in triple-negative breast cancer cell line MDA-MB-231. AU - De Blasio, Anna. AU - Di Fiore, Riccardo. AU - Morreale, Marco. AU - Carlisi, Daniela. AU - Drago-Ferrante, Rosa. AU - Montalbano, Mauro. AU - Scerri, Christian. AU - Tesoriere, Giovanni. AU - Vento, Renza. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Triple-negative breast cancer (TNBC) is a clinically aggressive form of breast cancer that is unresponsive to endocrine agents or trastuzumab. TNBC accounts for ∼10-20% of all breast cancer cases and represents the form with the poorest prognosis. Patients with TNBC are at higher risk of early recurrence, mainly in the lungs, brain and soft tissue, therefore, there is an urgent need for new therapies. The present study was carried out in MDA-MB-231 cells, where we assessed the role of caspase-8 (casp-8), a critical effector of death receptors, also involved in non-apoptotic functions. Analysis of casp-8 mRNA and protein levels indicated that they were ...

Background: Medicinal plants, especially examples rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Punica granatum (PG) (pomegranate) is a well known fruit in this context, but its cytotoxicity in cancer cells has not been extensively studied. Here, we investigated the antiproliferative properties of a peel extract of PG from Iran in different human cancer cells. Materials and Methods: A methanolic extract of pomegranate peel (PPE) was prepared. Total phenolic content(TPC) and total flavonoid conetnt (TFC) were determined by colorimetric assays. Antioxidant activity was determined by DPPH radical scavenging activity. The cytotoxicity of different doses of PPE (0, 5, 20, 100, 250, 500, |TEX|$1000{\mu}g/ml$|/TEX|) was evaluated by MTT assays with A549 (lung non small cell cancer), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer), and PC-3 (prostate adenocarcinoma) cells. Results: Significant (P|0.01) or very significant (P|0.0001)

TY - JOUR. T1 - Characterization of a newly established human pancreatic carcinoma cell line, UK Pan-1. AU - Fralix, Kimberly D.. AU - Ahmed, Mansoor M.. AU - Mattingly, Cynthia. AU - Swiderski, Carol. AU - McGrath, Patrick C.. AU - Venkatasubbarao, Kolaparthi. AU - Kamada, Nanao. AU - Mohiuddin, Mohammed. AU - Strodel, William E.. AU - Freeman, James W.. PY - 2000/5/1. Y1 - 2000/5/1. N2 - BACKGROUND. A highly tumorigenic cell line designated as UK Pan-1 was established in a surgically removed human pancreatic adenocarcinoma and characterized as having many of the genotypic and phenotypic alterations commonly found in pancreatic tumors. METHODS. The cell line was characterized by its morphology, growth rate in monolayer culture and soft agar, tumorigenicity in nude mice, and chromosomal analysis. Furthermore, the status of p53, Ki-ras mutation and transforming growth factor (TGF)-β receptor expression were determined. The characteristics of UK Pan-1 were compared with those of other commonly ...

TY - JOUR. T1 - Amurubicinol-induced eotaxin-3 expression in human NCI-H69 small cell lung carcinoma cells. AU - Niiya, Masami. AU - Niiya, Kenji. AU - Shibakura, Misako. AU - Asaumi, Noboru. AU - Yoshida, Chikamasa. AU - Tanimoto, Mitsune. PY - 2006/3. Y1 - 2006/3. N2 - We previously demonstrated the doxorubicin-induced expression of urokinase-type plasminogen activator (uPA), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α in human RCK8 lymphoma cells and NCI-H69 small cell lung carcinoma cells in which reactive oxygen species might be involved. Amurubicin hydrochloride (AMR), a novel derivative drug of doxorubicin, was recently introduced to clinical practice for treatment of lung cancer in Japan. Therefore, we investigated the effects of AMR on the expression of uPA and chemokines in NCI-H69 cells. AMR and its active form, amurubicinol hydrochloride (AMROH), both induced the expression of uPA, IL-8 and MCP-1 in H69 cells in a dose-dependent ...

TY - JOUR. T1 - Low oxygen tension increases mRNA levels of alpha 1 (I) procollagen in human dermal fibroblasts. AU - Falanga, Vincent. AU - Martin, Theresa A.. AU - Takagi, Hajime. AU - Kirsner, Robert S.. AU - Helfman, Todd. AU - Pardes, Jeffrey. AU - Ochoa, M. Sofia. PY - 1993/11. Y1 - 1993/11. N2 - Dermal fibroblasts exposed to low oxygen tension show upregulated synthesis of transforming growth factor-beta 1 (TCP-beta 1), an established stimulatory peptide in the formation of extracellular matrix proteins. In this report, procollagen synthesis was measured in cultures of confluent adult human dermal fibroblasts exposed to either standard (20%) or low (2%) oxygen tension. By Northern blot analysis the steady state levels of alpha 1 (I) procollagen mRNA were increased by 75 to 150% of control (standard oxygen) as early as 12 hours and more than 200% 96 hours after exposure of cells to low oxygen. Similar increases in procollagen mRNA levels were obtained in hypoxic fibroblast cultures in a ...

TY - JOUR. T1 - Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines. AU - Sekiya, S.. AU - Nunoyama, T.. AU - Shirasawa, H.. AU - Kimura, H.. AU - Kawata, M.. AU - Iijima, N.. AU - Sugimoto, Y.. AU - Tsuruo, T.. AU - Takamizawa, H.. PY - 1992/6. Y1 - 1992/6. N2 - To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ...

BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.

TY - JOUR. T1 - Comprehensive genetic characterization of human thyroid cancer cell lines. T2 - A validated panel for preclinical studies. AU - Landa, Iñigo. AU - Pozdeyev, Nikita. AU - Korch, Christopher. AU - Marlow, Laura A.. AU - Smallridge, Robert Christian. AU - Copland, John A III. AU - Henderson, Ying C.. AU - Lai, Stephen Y.. AU - Clayman, Gary L.. AU - Onoda, Naoyoshi. AU - Tan, Aik Choon. AU - Garcia-Rendueles, Maria E.R.. AU - Knauf, Jeffrey A.. AU - Haugen, Bryan R.. AU - Fagin, James A.. AU - Schweppe, Rebecca E.. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Purpose: Thyroid cancer cell lines are valuable models but have been neglected in pancancer genomic studies. Moreover, their misidentification has been a significant problem. We aim to provide a validated dataset for thyroid cancer researchers. Experimental Design: We performed next-generation sequencing (NGS) and analyzed the transcriptome of 60 authenticated thyroid cell lines and compared our findings with the known genomic defects ...

Full Text - Gomisin M2 isolated from Schisandra viridis A. C. Smith has potential anti-tumor effects on certain cancers, including breast cancer. However, only a few investigations have been conducted on the effects of Gomisin M2 on breast cancer stem cells (CSCs), which have the ability to self-renew and differentiate, as a possible strategy to resolve cancer cell resistance to apoptosis and to improve treatments. It is essential to investigate the effects of Gomisin M2 on breast cancer stem cells (BCSCs). In this study, we enriched breast cancer stem cells with CD44+/CD24- from MDA-MB-231 and HCC1806 cells through magnetic-activated cell sorting and cultured these in serum-free medium. The ability of Gomisin M2 to kill breast cancer stem cells was evaluated in vitro and in vivo. Gomisin M2 significantly inhibited the proliferation of the triple-negative breast cancer cell lines and mammosphere formation in breast CSCs and downregulated the Wnt/β-catenin self-renewal pathway.

TY - JOUR. T1 - Action mechanism and signal pathways of psidium guajava L. aqueous extract in killing prostate cancer LNCaP cells. AU - Chen, Kuan Chou. AU - Peng, Chiung Chi. AU - Chiu, Wen Ta. AU - Cheng, Yu Ting. AU - Huang, Guan Ta. AU - Hsieh, Chiu Lan. AU - Peng, Robert Y.. PY - 2010/2. Y1 - 2010/2. N2 - Aqueous extract of Psidium guajava L. budding leaves (PE) has been shown to possess anti-prostate cancer activity in a cell line model. We examined whether its bioactivity could be conserved either in the presence or the absence of synthetic androgen R1881. In both cases, PE was shown to inhibit LNCaP cell proliferation and down-regulate expressions of androgen receptor (AR) and prostate specific antigen (PSA). The cytotoxicity of PE was shown by enhanced LDH release in LNCaP cells. The flow cytometry analysis revealed cell cycle arrests at G0/G1 phase with huge amount of apoptotic LNCaP cells after treatment with PE for 48 h in a dose-responsive manner, which was also confirmed by TUNEL ...

BioAssay record AID 86084 submitted by ChEMBL: In vitro cytotoxicity against human colon adenocarcinoma cell line HT-29 at 5.9*10e-5M.

Oncolytic virotherapy for cancer treatment utilizes viruses for selective infection and death of cancer cells without any adverse effect on normal cells. We previously reported that the human respiratory syncytial virus (RSV) is a novel oncolytic virus against androgen-independent PC-3 human prostate cancer cells. The present study extends the result to androgen-dependent prostate cancer, and explores the underlying mechanism that triggers RSV-induced oncolysis of prostate cancer cells. The oncolytic effect of RSV on androgen-sensitive LNCaP human prostate cancer cells and on androgen-independent RM1 murine prostate cancer cells was studied in vitro in culture and in vivo in a xenograft or allograft tumor model. In vitro, cell viability, infectivity and apoptosis were monitored by MTT assay, viral plaque assay and annexin V staining, respectively. In vivo studies involved virus administration to prostate tumors grown in immune compromised nude mice and in syngeneic immune competent C57BL/6J mice. Anti

TY - JOUR. T1 - Altered gene expression profiles by sodium/iodide symporter gene transfection in a human anaplastic thyroid carcinoma cell line using a radioactive complementary dna microarray. AU - Gol Choe, Jae. AU - Kim, Yu Ri. AU - Kim, Ki Nam. AU - Choo, Hyo Jung. AU - Shin, Jae Hoon. AU - Lee, Yong Jin. AU - Chung, June Key. AU - Kim, Meyoung Kon. PY - 2005/12. Y1 - 2005/12. N2 - The sodium/iodide symporter (NIS) is a membrane glycoprotein that mediates active I uptake during the treatment of cancer of the thyroid gland and extrathyroidal tissues. NIS gene transfection, a gene-therapy modality, has been introduced in many types of cancer, such as prostate cancer and breast cancer, and has demonstrated a high potential for the treatment of non-thyroidal cancers. To investigate the pattern of NIS gene expression and provide evidence of its beneficial effects in human anaplastic cancer ARO cells by using a radioactive complementary DNA (cDNA) microarray.. AB - The sodium/iodide symporter ...

article{1fdd16d8-cade-46c6-b3bf-0719173f035c, abstract = {Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT2A) have been related to depression pathology. Specific 5-HT2A receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT2A receptor level. Here we show a direct effect of BDNF on 5-HT2A receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT2A receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50 ng/mL BDNF resulted in downregulation of 5-HT2A, but not of 5-HT1A, receptor protein levels. The BDNF-associated downregulation of 5-HT2A receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice ...

TY - JOUR. T1 - Metabolomic profiling of sodium fluoride-induced cytotoxicity in an oral squamous cell carcinoma cell line. AU - Sakagami, Hiroshi. AU - Sugimoto, Masahiro. AU - Tanaka, Shoji. AU - Onuma, Hiromi. AU - Ota, Sana. AU - Kaneko, Miku. AU - Soga, Tomoyoshi. AU - Tomita, Masaru. PY - 2014/4/1. Y1 - 2014/4/1. N2 - Sodium fluoride (NaF) is used in dentistry as a preventive agent for dental caries because of its ability to remineralize the tooth surface and its antibacterial effect. Although one of its target molecules in bacteria is enolase, its site of action in human cells has not been identified. The aim of this study was to identify target metabolites that are coupled to NaF-induced cytotoxicity in the HSC-2 human oral squamous cell carcinoma cell line. Cell viability, membrane integrity and apoptosis induction were analyzed by MTT assay, trypan blue exclusion and caspase-3 activation, respectively. Cells were treated with a minimal cytotoxic concentration of NaF for various times ...

Background aims. Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disorders (PTLD) belong to the most dreaded complications of immunosuppression. The efficacy of EBV-specific T-cell transfer for PTLD has been previously shown, yet the optimal choice of EBV-derived antigens inducing polyclonal CD4þ and CD8þ T cells that cover a wide range of human leukocyte antigen types and efficiently control PTLD remains unclear. Methods. A pool of 125 T-cell epitopes from seven latent and nine lytic EBV-derived proteins (EBVmix) and peptide pools of EBNA1, EBNA3c, LMP2a and BZLF1 were used to determine T-cell frequencies and to isolate T cells through the use of the interferon (IFN)-g cytokine capture system. We further evaluated the phenotype and functionality of the generated T-cell lines in vitro. Results. EBVmix induced significantly higher T-cell frequencies and allowed selecting more CD4þIFN-gþ and CD8þIFN-gþ cells than single peptide pools. T cells of all specificities ...

TY - JOUR. T1 - Gene expression profile of Li23, a new human hepatoma cell line that enables robust hepatitis C virus replication. T2 - Comparison with HuH-7 and other hepatic cell lines. AU - Mori, Kyoko. AU - Ikeda, Masanori. AU - Ariumi, Yasuo. AU - Kato, Nobuyuki. PY - 2010/12. Y1 - 2010/12. N2 - Human hepatoma cell line HuH-7-derived cells are currently the only cell culture system used for robust hepatitis C virus (HCV) replication. We recently found a new human hepatoma cell line, Li23, that enables robust HCV replication. Although both cell lines had similar liver-specific expression profiles, the overall profile of Li23 seemed to differ considerably from that of HuH-7. To understand this difference, the expression profile of Li23 cells was further characterized by a comparison with that of HuH-7 cells.Methods: cDNA microarray analysis using Li23 and HuH-7 cells was performed. Li23-derived ORL8c cells and HuH-7-derived RSc cells, in which HCV could infect and efficiently replicate, were ...

By Adam Bonislawski A team led by Swiss Federal Institute of Technology Zurich researcher Ruedi Aebersold has published a quantitative characterization of the proteome of the human tissue culture cell line U2OS.

TY - JOUR. T1 - Growth of an androgen‐sensitive human prostate cancer cell line, LNCaP, in nude mice. AU - Lim, David J.. AU - Liu, Xiang‐Lin ‐L. AU - Sutkowski, Debra M.. AU - Braun, Eva J.. AU - Lee, Chung. AU - Kozlowski, James M.. PY - 1993. Y1 - 1993. N2 - This study was undertaken to establish an androgen‐sensitive model of human prostatic carcinoma in nude mice. The androgen‐sensitive prostatic carcinoma cell line, LNCaP, was suspended in reconstituted basement membrane (Matrigel) and injected subcutaneously into nude mice. The LNCaP cell line was chosen for the study, because the cell line is androgen‐sensitive and secretes prostate specific antigen (PSA) into culture media. Following injection of 1 × 106 LNCaP cells with 0.25 ml of Matrigel, 88. of mice exhibited palpable tumor burdens after 12 weeks of observation. In addition, significant levels of PSA were observed in the serum of LNCaP‐bearing mice. Bilateral orchiectomy of mice resulted in tumor regression and ...

TY - JOUR. T1 - Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation. AU - Yang, K.. AU - Xue, J. J.. AU - Qiu, Y. H.. AU - Perez-Polo, J. R.. AU - Whitson, J.. AU - Faustinella, F.. AU - Kampfl, A.. AU - Zhao, X.. AU - Iwamoto, Y.. AU - Mu, X. S.. AU - Clifton, G.. AU - Hayes, R. L.. PY - 1996/12/31. Y1 - 1996/12/31. N2 - We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament ...

BioAssay record AID 80927 submitted by ChEMBL: Compound was tested for differentiation-inducing activity against human promyelocytic leukemia cell line HL-60.

TY - JOUR. T1 - Separation and analysis of subcellular organelles in a human promyelocytic leukemia cell line, HL-60. T2 - Application to the study of myeloid lysosomal enzyme synthesis and processing. AU - Nauseef, W. M.. AU - Clark, Robert A. PY - 1986. Y1 - 1986. N2 - We describe a system for analysis of the intracellular pathways in the biosynthesis and packaging of functionally important proteins in human myeloid cells. The human promyelocytic cell line HL-60 was used since peripheral blood neutrophils are terminally differentiated and do not actively synthesize protein. Cells were disrupted by nitrogen cavitation and subcellular organelles in postnuclear supernatant separated on a discontinuous gradient of Percoll modified to resolve organelles important in protein synthesis. This Percoll gradient separated azurophilic granules from less dense organelles and partially separated the less dense organelles from one another. Approximate densities of organelles identified by electron microscopy ...

TY - JOUR. T1 - Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with PYM to enhance translation of viral intronless mRNAs. AU - Boyne, James R.. AU - Jackson, Brian R.. AU - Taylor, Adam. AU - MacNab, Stuart A.. AU - Whitehouse, Adrian. PY - 2010/6/2. Y1 - 2010/6/2. N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) expresses numerous intronless mRNAs that are unable to access splicing-dependent cellular mRNA nuclear export pathways. To circumvent this problem, KSHV encodes the open reading frame 57 (ORF57) protein, which orchestrates the formation of an export-competent virus ribonucleoprotein particle comprising the nuclear export complex hTREX, but not the exon-junction complex (EJC). Interestingly, EJCs stimulate mRNA translation, which raises the intriguing question of how intronless KSHV transcripts are efficiently translated. Herein, we show that ORF57 associates with components of the 48S pre-initiation complex and co-sediments with the 40S ribosomal subunits. ...

Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services

TY - JOUR. T1 - Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines. AU - Jawhari, A. U.. AU - Noda, M.. AU - Farthing, M. J.G.. AU - Pignatelli, M.. PY - 1999/1/1. Y1 - 1999/1/1. N2 - Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, α, β and γ-catenin and p120(ctn), and of the adenomatous polyposis coil protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, α, β and γ-catenin, p120(ctn) and APC. Abnormalities of E-cadherin, α- and ...

Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of serum and used as a non-viral gene delivery system. To determine whether DOTAP and DC lipid DNA complexes could be obtained with increased transfection effciency in the presence of high serum concentrations, the composition of the complexes was varied systematically and a total of 162 different complexes were analyzed for transfection efficiency in the presence and absence of high serum concentrations. Increasing the ratio of DOTAP or DC to DNA led to a dose dependent enhancement of transfection efficiency in the presence of high serum concentrations up to a ratio of approximately 128 nmol lipid/μg DNA. Transfection efficiency could be further increased for all ratios of DOTAP and DC to DNA by addition of the DNA condensing agent protamine sulfate (PS). For DOTAP DNA complexes with ratios of ≤ 32

TY - JOUR. T1 - Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. AU - Reiter, Jill. AU - Drendel, Holli M.. AU - Chakraborty, Sujata. AU - Schellinger, Megan M.. AU - Lee, Men Jean. AU - Mor, Gil. PY - 2017/4/1. Y1 - 2017/4/1. N2 - Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need ...

Fingerprint Dive into the research topics of 'Retinoic acid inhibits IL-6-dependent but not constitutive STAT3 activation in Epstein-Barr virus-immortalized B lymphocytes.'. Together they form a unique fingerprint. ...

Adipose tissue-derived stem cells are a powerful tool for in vitro study of adult stem cell biology. So far, they have not been extensively used for gain or loss of function studies since they are resistant to most common transfection methods. Herein, we tested several classic transfection methods on human multipotent adipose tissue-derived stem (hMADS) cells. Our results showed that lipofectants and calcium phosphate were poorly efficient for transgene delivery in hMADS cells. In contrast, nucleofection, an electroporation-based method that is assumed to target plasmid DNA directly to the cell nucleus, led to a significant transient transgene expression in hMADS cells (up to 76% enhanced green fluorescent protein [EGFP]-positive cells were detected). Furthermore, after selection of hMADS cells that were nucleofected with a selectable plasmid coding for EGFP, stable EGFP expressing clones could be propagated in culture and efficiently induced to differentiate into EGFP-positive adipocytes and ...

TY - JOUR. T1 - Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines. AU - Bolt, Alicia M.. AU - Byrd, Randi M.. AU - Klimecki, Walter T.. PY - 2010/5/1. Y1 - 2010/5/1. N2 - Arsenic is a widespread environmental toxicant with a diverse array of molecular targets and associated diseases, making the identification of the critical mechanisms and pathways of arsenic-induced cytotoxicity a challenge. In a variety of experimental models, over a range of arsenic exposure levels, apoptosis is a commonly identified arsenic-induced cytotoxic pathway. Human lymphoblastoid cell lines (LCL) have been used as a model system in arsenic toxicology for many years, but the exact mechanism of arsenic-induced cytotoxicity in LCL is still unknown. We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to ...

The role of hepatitis B virus (HBV) X protein (HBx) in the regulation of HBV replication remains controversial. In the present study, the role of HBx in regulating HBV replication was initially investigated in both HepG2 and Huh7 in vitro cell lines with a transient transfection system. Next, the regions of HBx responsible for transcriptional transactivation and promotion of HBV replication were mapped in an HBV replication mouse model by in vivo transfection of a series of HBx expression plasmids. In an in vitro setting, HBx deficiency had little effect on HBV replication in Huh7 cells, but impaired HBV replication in HepG2 cells. In an in vivo setting, HBx had a strong enhancing effect on HBV transcription and replication. For the C-terminal two-thirds of the protein (amino acids [aa] 51 to 154) was required for this function of HBx, and the regions spanning aa 52 to 72 and 88 to 154 were found to be important for the stimulatory function of HBx on HBV replication. In conclusion, the role of HBx in

Cells, transfections, virus propagation, and antiviral agent. The cell lines used in this work were the class I MHC-negative Epstein-Barr virus-transformed B cell line 721.221 (.221), .221 cells transfected with the CEACAM1 cDNA (15), and the murine thymoma BW cell line, which lacks expression of α and β chains of the TCR. Stable transfection of .221 cells expressing CEACAM6 and CEACAM5 was performed by electroporation (0.23 kV, Cap [μF] 250 μF). The cDNA for CEACAM6 was amplified by RT-PCR and cloned into pcDNA3 expression vector, and the CEACAM5 cDNA was a kind gift from W. Zimmermann, Ludwig-Maximilians-University, Muenchen, Germany) Human foreskin fibroblasts (HFFs) were used for propagation and infection of human CMV strain AD169 (American Type Culture Collection, Manassas, Virginia, USA), as previously described (16). After a 1-hour period of virus adsorption to cells, 300 μg/ml of the CMV DNA polymerase inhibitor phosphonoformate (PFA; Sigma-Aldrich, St. Louis, Missouri, USA) was ...

TY - JOUR. T1 - PTH-related protein enhances LoVo colon cancer cell proliferation, adhesion, and integrin expression. AU - Shen, Xiaoli. AU - Falzon, Miriam. PY - 2005/2/15. Y1 - 2005/2/15. N2 - Parathyroid hormone-related protein (PTHrP) has been localized in human colon cancer tissue and cell lines. Tumor cell adhesion to extracellular matrix (ECM) proteins plays a major role in the invasion and metastasis of tumor cells, and is mediated via integrin subunits. The LoVo human colon cancer cell line was used as a model system to study the effects of PTHrP on cell proliferation and adhesion to ECM proteins found in normal liver. Clones of LoVo cells engineered to overexpress PTHrP by stable transfection with a PTHrP cDNA showed enhanced cell proliferation vs. control (empty vector-transfected) cells. PTHrP-overexpressing cells also showed significantly higher adhesion to collagen type I, fibronectin, and laminin, and enhanced expression of the ∀2, ∀5, ∀6, ∃1 and ∃4 integrin subunits. ...

Recombinant human sHer2 produced by HEK 293 and sHer2 isolated from the supernatant of the primary tumor cell culture from breast cancer patients was used in the experiments. The oligomeric status was estimated using the method of gel-filtration chromatography and electrophoresis. The stability of Her2 was determined by the change in the protein secondary structure under variation of temperature and pH values by means of CD spectroscopy. The inhibitory activity of sHer2 was analyzed in tests with Her2-expressing cell cultures. The content of sHer2 in the supernatant of primary tumor cell cultures from breast cancer patients was determined using the sandwich ELISA. Amplification of the HER2 gene in the primary tumor cells was verified employing the FISH. ...

Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene ...

c h ras oncogene, b h ras oncogene, g h ras oncogene, f h ras oncogene, v g ras oncogene, v b ras oncogene, v n ras oncogene, v j ras oncogene, v u ras oncogene, v y ras oncogene, v h eas oncogene, v h das oncogene, v h fas oncogene, v h tas oncogene, v h 5as oncogene, v h 4as oncogene, v h rzs oncogene, v h rss oncogene, v h rws oncogene, v h rqs oncogene, v h raa oncogene, v h raz oncogene, v h rax oncogene, v h rad oncogene, v h rae oncogene, v h raw oncogene, v h ras incogene, v h ras kncogene, v h ras lncogene, v h ras pncogene, v h ras 0ncogene, v h ras 9ncogene, v h ras obcogene, v h ras omcogene, v h ras ojcogene, v h ras ohcogene, v h ras onxogene, v h ras onvogene, v h ras onfogene, v h ras ondogene, v h ras oncigene, v h ras onckgene, v h ras onclgene, v h ras oncpgene, v h ras onc0gene, v h ras onc9gene, v h ras oncofene, v h ras oncovene, v h ras oncobene, v h ras oncohene, v h ras oncoyene, v h ras oncotene, v h ras oncogwne, v h ras oncogsne, v h ras oncogdne, v h ras oncogrne, v ...

On Thu, 9 Nov 1995, Gregory Bloom wrote: , Recent articles here regarding the significance of the telomere , in senescence have stated that the telomere is plenty long enough , at death in most organisms, Sorry but I do not remember the source, but from the reseach report sometime in the last year immortal cell lines were given antibodies to stop telomerase production if my memory is correct. All immortal cell lines died after total number of cell divisions for that species. I have know this for some time and have waited for someone else to post it so I would know the source. Ron Blue , My question is - what makes immortalized cell lines immortal? If you remove chromosome number one from a human cell it becomes immortal. Source Science (year unknow). , Do they suffer any of the cumulative damage from oxidation? yes if extreme , If so, are their repair mechanisms simply revved up beyond , normal 'mortal' cells? of course, immortal does not mean immortal. , If they have active telomerase, does , ...

Transformation of Rat-1 fibroblasts with the v-src oncogene leads to a 6- to 8-fold enhancement of the activity of the Ins(1,4,5)P3 3-kinase in cytosolic extracts [Johnson, Wasilenko, Mattingly, Weber and Garrison (1989) Science 246, 121-124]. This study confirms these results using another v-src-transformed Rat-1 cell line (B31 cells) and investigates the molecular mechanism by which pp60v-src activates Ins(1,4,5)P3 3-kinase. The mRNA and protein levels for two rat isoforms of Ins(1,4,5)P3 3-kinase were determined in the v-src-transformed cell line. Both the mRNA and protein levels for isoform A were elevated in v-src-transformed Rat-1 cells while those for isoform B were not significantly affected. Moreover, stable expression of either form of Ins(1,4,5)P3 3-kinase in the B31 v-src-transformed Rat-1 cell line did not result in tyrosine phosphorylation of Ins(1,4,5)P3 3-kinase A or B. These results suggest that at least one mechanism by which the v-src oncogene increases the activity of the ...

When we work with cell lines in the lab, we often work with HeLa cells. They can live in a vial of nutrients, and from a small sample you can grow a large