antibiotic resistance markers and future transformation selection technologies. Organisms resistant to ampicillin by virtue of. Klebsiella pneumoniae.. nitrofurantoin (66%) but less susceptible to ampicillin (4%) and ticarcillin (7%). but very resistant to. Klebsiella pneumoniae ATCC 700603 and Escherichia.Klebsiella oxytoca; Klebsiella pneumoniae; Legionella; Morganella morganii; Proteus rettgeri; Proteus vulgaris; Providencia; Pseudomonas; Serratia; Yersinia.Why is klebsiella resistant to ampicillin ampicillin hcpcs code ampicillin plus tobramycin order ampicillin bertibarots ampicillin droge.réservez vos places Lanfains 22 - BZH - France (près de St-Brieuc) Accueil Infos pratiques éditions passées partenaires Lorganisation Presse Contact.Ampicilline + gentamicine. les germes plus inhabituels tels que la Klebsiella pneumoniae,. Carr TL, Beazley DD et al. Antibiotic use in pregnancy and drug.Ampicilline G (1962) Ent robact ries (1964) C phalosporines 3G (1980) Ent robact. 2/ Klebsiella ...
TY - JOUR. T1 - Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. AU - Kim, Mi Na. AU - Yong, Dongeun. AU - An, Dongheui. AU - Chung, Hae Sun. AU - Woo, Jun Hee. AU - Lee, Kyungwon. AU - Chong, Yunsop. PY - 2012/4/1. Y1 - 2012/4/1. N2 - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four patients with no history of traveling abroad who stayed for 21 to 205 days in a tertiary care hospital. All were sequence type (ST) 340 and had nearly identical XbaI pulsed-field gel electrophoresis (PFGE) patterns. The bla NDM-1-carrying plasmids were in the IncN group, with sizes ranging from 50 to 200 kb. These findings suggest that NDMKP had already been introduced into South Korea before this clustering was found.. AB - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four ...
The pipeline guide provides a snapshot of the global therapeutic landscape of Klebsiella Pneumoniae Infections. The pipeline guide reviews pipeline therapeutics for Klebsiella Pneumoniae Infections by companies and universities/research institutes based on information derived from company and industry-specific sources.
It appears as a mucoid lactose fermenter on MacConkey agar. Klebsiella pneumonia ( KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. B) A total of 5, 475 samples collected from children resulted in laboratory- positive cultures; the 5 most frequently occurring bacterial species accounted for [ approximately equal to] 70% of total bacterial infections, and Klebsiella pneumoniae ( white bar) was the third most dominant ( 710 isolates). Klebsiella pneumoniae is a bacterium that causes UTIs. Pneumoniae is the most prevalent and clinically important. For example, Klebsiella must enter the respiratory ( breathing) tract to cause pneumoniae, or the blood to cause a bloodstream infection. Klebsiella pneumoniae și articulaţii. To get a Klebsiella infection, a person must be exposed to the bacteria. The organism resides in the upper respiratory tract and gastrointestinal tract of healthy individuals. Klebsiella pneumoniae is a bacterial organism that is responsible for causing ...
klebsiella. FAQ. Medical Information. Klebsiella pneumoniae; Klebsiella oxytoca; Enterobacteriaceae; Enterobacter;. Ciprofloxacin; HLA-B27 Antigen; Aminoglycosides.. Characterization of an IncN2-type bla NDM-1-carrying plasmid in Escherichia coli ST131 and Klebsiella pneumoniae ST11 and ST15 isolates in Thailand.ColonizaciÓn fecal por cepas de Klebsiella pneumoniae productoras de betalactamasas de espectro. and more than 90% were also susceptible to ciprofloxacin and.Community-acquired Klebsiella pneumoniae meningitis in an alcoholic patient with an infected pancreatic pseudocyst; a case report and review of literature.%T Urinary pharmacodynamics of low-dose ciprofloxacin and ofloxacin %A STEIN G. E. %A SCHOOLEY S. MIC = 4; Klebsiella pneumoniae, MIC = 4; Staphylococcus.Titre du document / Document title Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type β-lactamase-producing.Titre du document / Document title Extended-spectrum beta-lactamases ...
TY - JOUR. T1 - Prevention of colonization and infection by klebsiella pneumoniae carbapenemase-producing enterobacteriaceae in long-term acute-care hospitals. AU - Hayden, Mary K.. AU - Lin, Michael Y.. AU - Lolans, Karen. AU - Weiner, Shayna. AU - Blom, Donald. AU - Moore, Nicholas M.. AU - Fogg, Louis. AU - Henry, David. AU - Lyles, Rosie. AU - Thurlow, Caroline. AU - Sikka, Monica. AU - Hines, David. AU - Weinstein, Robert A.. PY - 2015/4/15. Y1 - 2015/4/15. N2 - Background. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (hereafter "KPC") are an increasing threat to healthcare institutions. Long-term acute-care hospitals (LTACHs) have especially high prevalence of KPC. Methods. Using a stepped-wedge design, we tested whether a bundled intervention (screening patients for KPC rectal colonization upon admission and every other week; contact isolation and geographic separation of KPC-positive patients in ward cohorts or single rooms; bathing all patients daily with ...
Abstract. We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14.. Keywords: Klebsiella pneumoniae Carbapenemase (KPC), multidrug resistance, whole genome sequencing ...
The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and the human host. Sequence analysis revealed novel plasmid-located virulence factors, including a cluster for glycogen synthesis. Glycogen production is considered to be one of the possible adaptive responses to long-term survival and growth in environments outside the host. Chromosomally-encoded virulence traits in the clone
Klebsiella pneumonia (KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. It is typically due to aspiration and alcoholism may be a risk factor, though it is also commonly implicated in hospital-acquired urinary tract infections, and COPD(chronic obstructive pulmonary disease) individuals Individuals with Klebsiella pneumonia tend to cough up a characteristic sputum, as well as having fever, nausea, tachycardia and vomiting. Klebsiella pneumonia tends to affect people with underlying conditions, such as alcoholism. The cause of the condition Klebsiella pneumonia is Klebsiella pneumoniae which is gram-negative, as well as rod-shaped, glucose-fermenting, facultative anaerobic bacterium. In terms of the pathophysiology of Klebsiella pneumonia we see neutrophil myeloperoxidase defense against K P.Oxidative inactivation of elastase is involved, while LBP helps transfer bacteria cell wall elements to the cells. Klebsiella resistant strains have been recorded in USA with a ...
TY - CONF. T1 - Successful treatment of KPC-3 Klebsiella Pneumoniae ST258 clone with a combination of high-dose tigecycline and colistin in ICU: a case series report.. AU - Gulotta, Gaspare. AU - Raineri, Santi Maurizio. AU - Bonura, Celestino. AU - Pantuso, Gianni. AU - Cortegiani, Andrea. AU - Giarratano, Antonino. AU - Agrusa, Antonino. AU - Di Carlo, Paola. AU - Cocorullo, Gianfranco. AU - Giammanco, Anna. AU - Mammina, Caterina. PY - 2011. Y1 - 2011. N2 - INFECTIONS CAUSED BY KLEBSIELLA PNEUMONIAE SEQUENCE TYPE 258 PRODUCING K. PNEUMONIAE CARBAPENEMASE 3 (KPC-Kp)HAVE WIDELY EMERGED AND BOTH INDIVIDUAL CASES AND OUTBREAKS OF COLONIZATION OR INFECTION HAVE BEEN REPORTED IN PALERMO, ITALY.OBIETTIVO: THIS IS A RETROSPECTIVE CASE SERIES THAT DESCRIBES THE CLINICAL AND MICROBIOLOGIC OUTCOMES OF 16 PATIENTS WHO RECEIVED A COMBINATION OF HIGH-DOSE TIGECYCLINE AND COLISTIN FOR TREATMENT OF VAP (4 CASES) AND SEVERE BACTERAEMIA (12 CASES) DURING THE YEARS 2009-2011. 11 OUT OF THE 16 CASES WERE POST ...
Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies. Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages. Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against 254 clinical Enterobacteriaceae strains including multidrug resistant Klebsiella isolates producing extended-spectrum beta-lactamases (ESBLs). Based on their lytic potential, six of the phages were further characterised for burst size, physicochemical properties and sensitivity to
Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies. Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages. Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against 254 clinical Enterobacteriaceae strains including multidrug resistant Klebsiella isolates producing extended-spectrum beta-lactamases (ESBLs). Based on their lytic potential, six of the phages were further characterised for burst size, physicochemical properties and sensitivity to
Klebsiella pneumoniae ATCC ® BAA-1705D-5™ Designation: Genomic DNA from Klebsiella pneumoniae strain ART 2008133 TypeStrain=False Application: Source culture is Modified Hodge Test (MHT) positive control designation Source culture produces Klebsiella pneumoniae carbapenemase (KPC)
Klebsiella Pneumoniae Infections - Market Insights, Epidemiology and Market Forecast - 2025 is a market research report available at US $5750 for a Single User PDF License from RnR Market Research Reports Library.
Background and Objective: The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae. The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic methods. Method: Out of 800 strains, 270 K. pneumoniae strains (33.7%), were obtained. Antibiotic susceptibility test was performed by disk diffusion method in accordance with CLSI guidelines. Carbapenem resistant strains were identified by the Modified Hodge Test based on CLSI instruction and PCR for surveying the presence of bla-KPC gene. Results: A total 270 K. pneumoniae strains were collected. Antibiotic susceptibility test results showed the highest and lowest resistance was related to piperacillin (60.6%) and carbapenems (14.6%) respectively. 80.5% (33 of 41) isolates were positive by MHT, but all of them (100%) were negative for amplification of the bla-KPC
To the Editor: Until a few years ago, the most frequent microbiologically documented cause of severe bloodstream infections among patients with hematologic malignancies was gram-positive bacteria (1). However, over the years, gram-negative bacteria have become the main infectious cause of death among patients with hematologic malignancies, and rates of different phenotypes associated with antimicrobial drug resistance are increasing (2). This trend could be the result of increasing empirical use of antimicrobial drug therapy and prophylaxis and use of new, more effective antimicrobial drugs. In particular, over the past few years at our hospital (Agostino Gemelli Teaching Hospital, Rome, Italy), we have observed a progressive increase in bloodstream infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp), which are responsible for a dramatic new scenario.. We reviewed records of all patients affected by hematologic malignancies who were admitted to the hospital ...
Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence ...
Carbapenemase-producing Enterobacteriaceae (CPE) have emerged in recent years as being among of the most important threats to public health. CPE have been detected in almost all European countries, although with a highly variable geographical distribution (1). Some high-risk clones of Klebsiella pneumoniae, mainly KPC-producing sequence type 258 (ST258) but also OXA-48-producing ST395 or ST15, have been implicated in the worldwide spread of carbapenemases (2-4).. Previous studies have shown that the population of Escherichia coli strains resistant to amoxicillin-clavulanic acid is less diverse than the susceptible population (5). Little is known about the population structure of carbapenemase-producing K. pneumoniae in comparison with the carbapenem-susceptible population. The aim of this study was to test the hypothesis that the carbapenemase-producing K. pneumoniae strains isolated in Spanish hospitals constitute a distinct and more homogeneous population than the carbapenemase-susceptible ...
Researchers at Queens University Belfast together with the University of Vienna have discovered that treatment for the antibiotic resistant bacteria Klebsiella pneumoniae could lie within our bodies natural defences. Multidrug resistance of microbes poses a serious global threat to human health. Globally, 700,000 people die every year due to antimicrobial resistance. The bacteria Klebsiella pneumoniae causes a number of infections including sepsis, urinary tract infections and pneumonia. As Klebsiella becomes more resistant to antibiotics, these common infections are becoming increasingly difficult to treat, which has led to the World Health Organisation recently declaring an urgent need for new therapeutics to be discovered for Klebsiella.. Professor Jose Bengoechea from the Wellcome-Wolfson Institute for Experimental Medicine at Queens University Belfast and one of the lead researchers explains: "Klebsiella pneumoniae is of particular concern as it can cause infections such as bladder ...
BioAssay record AID 1085598 submitted by ChEMBL: Antibacterial activity against Klebsiella pneumoniae at 1 ug/ml after 24 hr by well diffusion method.
Background. During 2006, Israeli hospitals faced a clonal outbreak of carbapenem-resistant Klebsiella pneumoniae that was not controlled by local measures. A nationwide intervention was launched to contain the outbreak and to introduce a strategy to control future dissemination of antibiotic-resistant bacteria in hospitals.. Methods. In March 2007, the Ministry of Health issued guidelines mandating physical separation of hospitalized carriers of carbapenem-resistant Enterobacteriaceae (CRE) and dedicated staffing and appointed a professional task force charged with containment. The task force paid site visits at acute-care hospitals, evaluated infection-control policies and laboratory methods, supervised adherence to the guidelines via daily census reports on carriers and their conditions of isolation, provided daily feedback on performance to hospital directors, and intervened additionally when necessary. The initial intervention period was 1 April 2007-31 May 2008. The primary outcome measure ...
Results: Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum β-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates ...
Klebsiella pneumoniae is a frequent cause of infectious outbreaks in Neonatal Intensive Care Units (NICUs). The aim of this paper is to describe an outbreak occurred in a 13-bed NICU and the control measures adopted in order to interrupt the chain of transmission. We described the microbiological investigations, the NICU staff compliance to the infection control measures by means of a specifically designed check-list and the control measures adopted. Six cases of primary bloodstream infections sustained by ampicillin/piperacillin-resistant Klebsiella pneumoniae were observed over a two-month period. One culture obtained from a 12% saccarose multiple-dose solution allowed the growth of Klebsiella pneumoniae. During the inspections performed by the Hospital Infection Control Team, using the check-list for the evaluation of the NICU staff compliance to the infection control measures, several breaches in the infection control policy were identified and control measures were adopted. In our case the
Klebsiella pneumoniae is gram negative rod which present with large mucoid capsulre. The common virulence factors of klebsiella pneumonia may include protease, cell wall endotoxin and mucoid capsule.
Several bacterial populations areknownto be containing some fraction of cells which survive exposure to antibiotics and harsh environment, are called as persister cells. This fraction of cells is very small generally ranging from 10-7 to 10-5. The mechanism of persister formation is not yet clearly understood although expression of toxin-antitoxin (TA) pairs of proteins has been found to be associated with persister formation. Klebsiella pneumoniae is also shown to produce persister cells by prolonged exposure to ampicillin.In this study, we have identified a pair of proteins, hipA and hipB, of TA system in Klebsiella pneumoniae. The proteins have 70% and 60% sequence similarity respectively with their homologous proteins from E. coli. hipA and hipB associate together to regulate survival of persister cells by binding to DNA in unfavourable conditions. Both hipA and hipB proteins from Klebsiella pneumoniae were cloned, expressed and purified. The clones were over expressed in fusion with His-tag ...
Schwaber et al identified risk factors for acquisition of carbapenem-resistant Klebsiella pneumoniae in a hospitalized patient. These can help to identify a patient who should be screened for carriage. The authors are from Tel Aviv Sourasky Medical Center.
Introduction: The aim of this study was to investigate the presence of carbapenemase production and carbapenem resistance mechanisms in 47 carbapenem resistant Klebsiella pneumoniae isolates by phenotypic confirmatory tests and molecular assay.. Methodology: Carbapenem resistance genes KPC, OXA-48 and NDM were investigated with the BD MAX CRE assay kit in the BD MAX real time PCR instrument. Modified Hodge test, MBL gradient strip test, D70C Carbapenemase Detection Set, Temocillin gradient strip test methods were used as phenotypic confirmatory tests. Clonal relationship between study isolates was investigated with pulsed-field gel electrophoresis.. Results: Analysis with BD MAX CRE assay revealed OXA-48 positivity in 17 (36%) strains, NDM positivity in 6 (13%) strains and coexistence of OXA-48 + NDM positivity in 8 (17%) strains. In 16 (34%) strains, none of the KPC, OXA-48 and NDM genes were detected. While MHT was the most sensitive phenotypic confirmatory test, D70C disc set had not been ...
Sequences of 11 amino acids belonging to the KPN_00363 protein and KPN_00459 protein from Klebsiella pneumoniae MGH 78578 which was previously identified as potentially immunogenic was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Overall design: 26 peptides, two representing the original sequences, 22 peptides with each residue replaced by alanine (or glycin, if the original sequence contained alanine) as well as two related peptides were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was seperated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interaction rabbit polyclonal IgG to K. pneumoniae (Abcam ab20947) was used, while non-specific binding to the epitope sequences was checked using rabbit polyclonal IgG to E. coli (Abcam ab137967) and S. enterica
Objective(s): Infections due to carbapenemase-producing Klebsiella pneumoniae are associated with high morbidity and mortality. In this study, we report a hospital outbreak due to co-producing OXA-48 and NDM-1 K. pneumoniae clone. The aim of the study is to investigate the clonal relationship of strains, risk factors of outbreak and infection control measures.Materials and Methods: Once an outbreak was suspected at the end of December 2017 in our intensive care unit (ICU), carbapenem resistance K. pneumoniae identified in patients specimens. An outbreak analysis was begun to determine the risk factors and dissemination of the cases. A case-control study was conducted to determine the risk factors. To control the outbreak; tight contact prevention, good clean-up the medical devices and hospital environment, were done. Staff training programs such as hand hygiene, disinfection, wearing aprons, good cleaning were created. Carbapenem resistance genes determined by PCR. Clonal relationships of strains
We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the …
The emergence of multidrug-resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of K. pneumoniae isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 K. pneumoniae strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98 %) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the K. pneumoniae strains harboured bla SHV, 19 harboured bla CTX-M, 5 harboured bla OXA-1 and 4 harboured bla TEM-1. Class 1 integron-encoded intI1 integrase was detected in 21 of the 51 K. pneumoniae strains and amplification of the integron 5′CS region showed the presence of several known antibiotic resistance gene
TY - JOUR. T1 - Genomic epidemiology of Klebsiella pneumoniae in Italy and novel insights into the origin and global evolution of its resistance to carbapenem antibiotics. AU - Gaiarsa, Stefano. AU - Comandatore, Francesco. AU - Gaibani, Paolo. AU - Corbella, Marta. AU - Valle, Claudia Dalla. AU - Epis, Sara. AU - Scaltriti, Erika. AU - Carretto, Edoardo. AU - Farina, Claudio. AU - Labonia, Maria. AU - Landini, Maria Paola. AU - Pongolini, Stefano. AU - Sambri, Vittorio. AU - Bandi, Claudio. AU - Marone, Piero. AU - Sasserac, Davide. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Klebsiella pneumoniae is at the forefront of antimicrobial resistance for Gram-negative pathogenic bacteria, as strains resistant to third-generation cephalosporins and carbapenems are widely reported. The worldwide diffusion of these strains is of great concern due to the high morbidity and mortality often associated with K. pneumoniae infections in nosocomial environments. We sequenced the genomes of 89 K. pneumoniae strains ...
A new series of extended-spectrum β-lactamase (ESBL) enzymes, cefotaximases (CTX-M), resulting in higher MICs of cefotaxime and ceftriaxone than of ceftazidime, has been discovered in several members of the family Enterobacteriaceae and in various countries (10). A previous study in southern Taiwan (11) showed that CTX-M-3 was the predominant ESBL among clinical isolates of Escherichia coli. We have also discovered that 87 of 211 (41%) ESBL-producing Klebsiella pneumoniae isolates from Taiwan expressed a CTX-M phenotype (ceftriaxone MIC, ≥16 μg/ml; ceftazidime MIC, ≤8 μg/ml [unpublished data]). CTX-M enzymes have not been previously identified in klebsiellae from Taiwan. In the present study, we determined the CTX-M identification by nucleotide sequencing, the distribution of MICs of various broad-spectrum β-lactams, and the molecular epidemiology of these CTX-M-producing strains.. A total of 211 clinical isolates of K. pneumoniae producing probable ESBLs (6) were recovered from 24 ...
The treatment outcome of 35 cases of bacteremia due to Klebsiella pneumoniae isolates producing TEM-52 extended-spectrum β-lactamase was studied. Twenty-eight cases, classified as "nonfatal disease" using the McCabe and Jackson classification, were investigated with regard to ciprofloxacin and imipenem response. Because ciprofloxacin was active in vitro against 21 of 28 isolates, only the treatment outcome of the ciprofloxacin-susceptible subgroup was evaluated. Eight of 10 cases occurred in patients who experienced a complete response to imipenem; 2 of 10 failed to respond. In contrast, only 2 of 7 cases had a partial response to ciprofloxacin, and, in 5 of 7 cases, the treatment failed. Statistical analysis revealed a significant difference in the treatment outcome of the 2 groups (P = .03). Because the isolates had minimum inhibitory concentrations of ciprofloxacin close to the susceptibility breakpoint, treatment failure could be ascribed to the inability of the drug to reach therapeutic ...
Other names: ATCC 13883, Bacillus pneumoniae, Bacterium pneumoniae crouposae, CCUG 225, CIP 82.91, DSM 30104, HAMBI 450, Hyalococcus pneumoniae, IFO 14940, K. pneumoniae, Klebsiella pneumoniae, Klebsiella sp. M-AI-2, Klebsiella sp. PB12, Klebsiella sp. RCE-7, LMG 2095, NBRC 14940, NCTC 9633 ...
Other names: ATCC 13883, Bacillus pneumoniae, Bacterium pneumoniae crouposae, CCUG 225, CIP 82.91, DSM 30104, HAMBI 450, Hyalococcus pneumoniae, IFO 14940, K. pneumoniae, Klebsiella pneumoniae, Klebsiella sp. M-AI-2, Klebsiella sp. PB12, Klebsiella sp. RCE-7, LMG 2095, NBRC 14940, NCTC 9633 ...
Klebsiella pneumoniae bacterium. Coloured transmission electron micrograph (TEM) of a section through a Klebsiella pneumoniae bacterium. K. pneumoniae is one of several bacteria that can cause pneumonia (lung inflammation). It causes a severe form that can be a complication of chronic lung diseases. K. pneumoniae is also a multidrug-resistant bacterium that can cause severe hospital-acquired infections. - Stock Image C026/3555
Klebsiella pneumoniae is a leading cause of severe infections in humans and dairy cows, and these infections are rapidly becoming untreatable due to the emergence of multidrug-resistant (MDR) strains. However, little is known about the relationship between bovine and human K. pneumoniae isolates at the genome population level. Here, we investigated the genomic structures, pangenomic profiles, virulence determinants, and resistomes of 308 K. pneumoniae isolates from humans and dairy cows, including 96 newly sequenced cow isolates. We identified 177 functional protein families that were significantly different across human and bovine isolates; genes expressing proteins related to metal ion (iron, zinc, and calcium) metabolism were significantly more prevalent among the bovine isolates. Siderophore systems were found to be prevalent in both the bovine and the human isolates. In addition, we found that the Klebsiella ferric uptake operon kfuABC was significantly more prevalent in clinical mastitis ...
CTX-M extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates are infrequently reported in the United States. In this study, we analyzed nonduplicate ESBL-producing K. pneumoniae and Escherichia coli clinical isolates collected during 2005-2012 at a tertiary care medical center in suburban New York City, USA, for the presence of blaCTX-M, blaSHV, blaTEM, and blaKPC genes. Despite a high prevalence of blaCTX-M genes in ESBL-producing E. coli since 2005, blaCTX-M genes were not detected in K. pneumoniae until 2009. The prevalence of CTX-M-producing K. pneumoniae increased significantly over time from 1.7% during 2005-2009 to 26.4% during 2010-2012 ( ...
Discussion. Incidence and trends of K. pneumoniae HAI. Yogaraj et al.[5] found that in their experience, in Washington DC, bloodstream infections accounted for 28% of HAI in the PICU, followed by ventilator-associated pneumonias, which accounted for a further 21%. Lakshmi et al.[6] reported on 116 episodes of bloodstream infection in 86 patients in India in 2006, with an incidence of 31.2 episodes/1 000 patient days. Marra et al.[7] found that K. pneumoniae ranked among the top ten pathogens causing bloodstream infection in the USA and Canada, and that it was the third most prevalent pathogen isolated in Latin America.. K. pneumoniae HAI is a significant problem in the delivery of intensive care services, and prolongs hospital stay. In this study, there was an incidence for K. pneumoniae HAI of 10/1 000 admissions, with a range of 0 - 28.8/1 000 patients per annum once the PICU was fully commissioned. Unfortunately, as this was a retrospective study, we were unable to access data on patient ...
This SuperSeries is composed of the following subset Series: GSE35746: Comparative analysis of regulatory elements between Escherichia coli and Klebsiella pneumoniae by genome-wide transcription start site profiling [tiling arrays] GSE35821: Comparative analysis of regulatory elements between Escherichia coli and Klebsiella pneumoniae by genome-wide transcription start site profiling [TSS-Seq] Refer to individual Series
Klebsiella pneumoniae is one of the normal bacterial flora of the intestinal tract. It is the second most commonly found bacteria in a persons gut after Escherichia coli. If this bacteria manages to get out of the gut, it can lead to some serious medical issues. This kind of opportunistic pathogen is known to cause urinary tract infections as well as the severe respiratory illness, such as pneumonia. When it increases entry into the lungs, it rapidly divides as well as leads to numerous harmful Klebsiella pneumoniae symptoms. In the following paragraphs, we shall have a look at this particular bacterial agent and find out about its pathophysiology related to pneumonia ...
TY - JOUR. T1 - FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. AU - Hsueh, Kuang Lung. AU - Yu, Liang Kun. AU - Chen, Yung Han. AU - Cheng, Ya Hsin. AU - Hsieh, Yin Cheng. AU - Ke, Shyue chu. AU - Hung, Kuo Wei. AU - Chen, Chun Jung. AU - Huang, Tai Huang. PY - 2013/10/18. Y1 - 2013/10/18. N2 - Iron is essential for pathogen survival, virulence, and colonization. Feo is suggested to function as the ferrous iron (Fe2+) transporter. The enterobacterial Feo system is composed of 3 proteins: FeoB is the indispensable component and is a large membrane protein likely to function as a permease; FeoA is a small Src homology 3 (SH3) domain protein that interacts with FeoB; FeoC is a winged-helix protein containing 4 conserved Cys residues in a sequence suitable for harboring a putative iron-sulfur (Fe-S) cluster. The presence of an iron-sulfur cluster on FeoC has never been shown experimentally. We report that under anaerobic conditions, the recombinant Klebsiella pneumoniae FeoC (KpFeoC) ...
Klebsiella pneumoniae glycoprotein: extract from Klebsiella pneumoniae; composed of 2 glycoproteins MW 350,000 & 95,000; restores & enhances delayed cutaneous hypersensitivity in cancer patients & reduces number & length of infectious episodes in chronic bronchitis patients
The articles reports on the discovery of a carbapenem-resistant Klebsiella pneumoniae strain producing a Verona integron-encoded metallo-beta-lactamase (VIM) carbapenemase. The strain, not common among Enterobacteriaceae in the U.S., was detected from an American tourist who was vacationing in Greece. Initial diagnosis was Clostridium difficile infection but was proven otherwise when the patient was transferred to a U.S. hospital. No other patients in the hospital was found to have the strain ...
Objective: Multidrug-resistant Enterobacteriaceae pose a serious infection control challenge and have emerged as a public health threat. We examined national trends in the proportion of Klebsiella pneumoniae isolates resistant to carbapenems (CRKP) and third-generation cephalosporins (G3CRKP).. Design and Setting: Retrospective analysis of approximately 500,000 K. pneumoniae isolates cultured between January 1999 and July 2010 at 287 clinical laboratories throughout the United States.. Methods: Isolates were defined as CRKP if they were nonsusceptible to 1 or more carbapenems and were defined as G3CRKP if they were nonsusceptible to ceftazidime, ceftriaxone, or related antibiotics. A multivariable analysis examined trends in the proportion of resistant isolates, adjusting for age, sex, isolate source, patient location, and geographic region.. Results: The crude proportion of CRKP increased from less than 0.1% to 4.5% between 2002 and 2010; the frequency of G3CRKP increased from 5.3% to 11.5% ...
Klebsiella pneumoniae is now recognized as an urgent threat to human health because of the emergence of multidrug-resistant strains associated with hospital outbreaks and hypervirulent strains associated with severe community-acquired infections. K. pneumoniae is ubiquitous in the environment and can colonize and infect both plants and animals. However, little is known about the population structure of K. pneumoniae, so it is difficult to recognize or understand the emergence of clinically important clones within this highly genetically diverse species. Here we present a detailed genomic framework for K. pneumoniae based on whole-genome sequencing of more than 300 human and animal isolates spanning four continents. Our data provide genome-wide support for the splitting of K. pneumoniae into three distinct species, KpI (K. pneumoniae), KpII (K. quasipneumoniae), and KpIII (K. variicola). Further, for K. pneumoniae (KpI), the entity most frequently associated with human infection, we show the ...
Table 4: Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing|i| Klebsiella pneumoniae|/i| Compared to Nonproducing Isolates
Eighteen K. pneumoniae strains were identified from patients: seven and nine strains from clinical and screening specimens respectively; and two strains from ventilator touchscreen and suction device manometer. M13 fingerprint analysis revealed closely related strains confirmed by MLST (ST15) performed in selected strains. All of the K. pneumoniae isolates had the same pattern of multiresistance.Molecular experiments revealed that CTX-M-15and SHV-28 were the prevalentESBLs. The outbreak was controlled and eliminated by a combination of intensive infection control measures and rigorous local surveillance. These safeguards remain in place and no outbreaks were detected since January 2010. ...
7.24.14. Selective Micro Technologies demonstrated pure chlorine dioxide successfully kills Carbapenem Resistant Klebsiella pneumoniae at greater than 99.9999%. The test was also conducted according to EPA registration standards using active ingredient at the lowest certified limit (LCL). Test demonstrated successful efficacy at 10 minute contact time. According to CDC, "CRE, which stands for carbapenem-resistant Enterobacteriaceae, are a family of germs that are difficult to treat because they have high levels of resistance to antibiotics. Klebsiella species and Escherichia coli (E. coli) are examples of Enterobacteriaceae, a normal part of the human gut bacteria, that can become carbapenem-resistant." CRE bacteria are highly contagious and marked by severe illness or even death. For more information click here.. ...
Abstract : Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedis biofield treatment on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into two groups i.e. control and biofield treated. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of MDR K. pneumonia isolates. MIC results showed an alteration in 30% of ...
Infection by Klebsiella pneumonia producing class A carbapenemases is a major clinical and public health problem in Israel and worldwide. The aim of this study is to determine the safety and efficacy of alteration of the gut microflora by probiotics, alone or in combination with mechanical bowel cleansing, as a strategy to eradicate colonization of the gastrointestinal tract by CRKP. We hypothesize that administration of probiotics will decrease the rate of GI colonization by CRKP ...
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Multiple drug resistance (MDR), multidrug resistance or multiresistance is antimicrobial resistance shown by a species of microorganism to multiple antimicrobial drugs. The types most threatening to public health are MDR bacteria that resist multiple antibiotics; other types include MDR viruses, fungi, and parasites (resistant to multiple antifungal, antiviral, and antiparasitic drugs of a wide chemical variety). Recognizing different degrees of MDR, the terms extensively drug resistant (XDR) and pandrug-resistant (PDR) have been introduced. The definitions were published in 2011 in the journal Clinical Microbiology and Infection and are openly accessible. Common multidrug-resistant organisms are usually bacteria: Vancomycin-Resistant Enterococci (VRE) Methicillin-Resistant Staphylococcus aureus (MRSA) Extended-spectrum β-lactamase (ESBLs) producing Gram-negative bacteria Klebsiella pneumoniae carbapenemase (KPC) producing Gram-negatives Multidrug-Resistant gram negative rods (MDR GNR) MDRGN ...
1. Center for Disease control and prevention. National Nosocomial Infections Surveillance 9NNIS0 System report, data summary from January 1992 through June 2003. Am J Infect Control 2003:31:481-98 2. Woodford N, Tierno PM Jr, Young K, Tysall L, Palepou MF, Ward E, Painter RE, Suber DF, Shungu D, Silver LL, Inglima K, Kornblum J, Livermore DM. Antimicrobial Agents Chemother. Outbreak of Klebsiella pneumonia producing a new carbapenem-hydrolyzing class a beta-lactamase, KPC-3, in a New York Medical Center.2004;48(12):4793-9. 3. Bradford PA, Bratu S, Urban C, Visalli M, Mariano N, Landman D, Rahal JJ, Brooks S, Cebular S, Quale J Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 beta-lactamases in New York City. Clin Infect Dis. 2004 1;39(1):55-60 ...
Background:. Emerging carbapenem-resistant enterobacteriacae makes it difficult to treat many gram-negative bacterial infection. We recently experienced a hospital outbreak of Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella pneumoniae (KPN) linked with an index case of community-acquired KPC-producing KPN infection. An outbreak investigation and whole genome sequencing analysis were performed to trace the outbreak and investigate the molecular characteristics of the isolates.. Methods:. Six cases of KPC-producing KPN were identified within the period October 2014 to February 2015. An epidemiological investigation and Pulsed field gel electrophoresis (PFGE) analysis showed 3 linked cases of which index case was community acquired. Active surveillance culture for exposed patients identified one more case with KPC-producing KPN colonization. Whole genome sequencing analysis for 4 linked cases were performed using a combination of Illumina Genome Analyzer (Illumina, San Diego, CA, ...
Klebsiella pneumoniae is an opportunistic, environmental organism that can cause intramammary infections in dairy cows (15, 32). The environment can contain a large variety of K. pneumoniae strains with pathogenic potential (26, 30), and mastitis cases within a dairy herd are usually caused by many different strains (3, 23-25). Predominance of a single K. pneumoniae strain, such as RAPD type A in the first outbreak of CM described here, is unusual. Predominance of a single strain can result from several mechanisms: (i) contagious transmission of the strain between cows, (ii) predominance of the strain in the environment, or (iii) an increased ability of an environmental strain to cause udder infections. Mechanisms 1 and 2 are often thought to be mutually exclusive but could occur together if infected cows contaminate the environment with milk with high pathogen loads, resulting in the subsequent exposure of other cows to the same bacterial strain (35). Thus, the relative abundance of RAPD type A ...
Klebsiella pneumoniae ATCC ® BAA-1705™ Designation: ART 2008133 TypeStrain=False Application: Modified Hodge Test (MHT) positive control designation Respiratory research Multi-drug testing
We studied antimicrobial-resistant Klebsiella pneumoniae for 1998-2010 by using data from The Surveillance Network. Susceptibility results (n = 3,132,354) demonstrated significant increases in resistance to all antimicrobial drugs studied, except tetracycline. Cross-resistance among carbapenem-resistant K. pneumoniae was lower for tetracycline and amikacin ...
Concluding remarks.The rising incidence of ESBL- and KPC-producing pathogens presents challenges in the empirical therapy for Gram-negative bacterial infections. Acknowledging the increased mortality rates with ineffective initial therapy (13, 16), the timely recognition of ESBL- and KPC-producing organisms may have a positive impact on clinical outcomes. Nucleic acid microarrays can be important in achieving this end.. In this study, the nucleic acid microarray technology (Check-KPC/ESBL kits) demonstrated accurate and reproducible results with regard to the presence of ESBL and KPC genes within the Enterobacteriaceae (2, 3, 8, 9). Current practice requires 24 h of adequate growth prior to testing on the microarray system. Our study shows that the Check-KPC/ESBL kit could be employed with DNA extracted directly from blood cultures before species identification. This could reduce the notification time by as much as 18 to 20 h. Depending on the method used, the time savings, costs of materials, ...
Efficient identification methods for laboratory testing, active surveillance and screening of patients are therefore in high need to avoid spreading of CRKP. The MBT Subtyping Module looks automatically for a blaKPC related peak in the sample spectrum after successful identification of the Enterobacteriaceae listed below. If present, the MALDI Biotyper will report this sample as a presumptive blaKPC positive. ...
INTRODUCTION: Respiratory tract infections are severe and most common types of infection treated by medical practitioners all over the world. Nosocomial pneumonia is the second most common infection, causing high morbidity and mortality and about 80% of nosocomial infections caused by Klebsiella pneumoniae are due to multidrug-resistant strains.. The emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial therapies 1. Important causes of Gram-negative resistance includes extended-spectrum β-lactamases (ESBLs) in Klebsiella pneumoniae, high level third-generation cephalosporin (Amp C) β-lactamase resistance among Enterobacter species observed in pneumonia.. Recent data suggest that because of ESBLs and high-level amp C β-lactamase resistances, use of third-generation cephalosporins may be ineffective in many patients with nosocomial infections 2. EDTA is a polyamino carboxylic acid, a colorless water-soluble metallo-chelator and is known to ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Les bêta -lactamines. ampicilline (po), amoxicilline. important de connaître les antécédents dallergie à une bêta lactamine et de tenir compte des.La première étape de la réaction enzymatique variera en fonction du substrat (affinité) tels pénicilline G, oxacilline, ampicilline. beta-lactamase TLA-2). 6.beta-lactamase-producing Klebsiella pneumoniae infection in. generation cephalosporins, ceftazidime, ampicillin, aminoglycosides and vancomycin/teicoplanin were.Original Article Antimicrobial. examined for production of beta-lactamase using a. Ampicillin (E) 26.7 1.3 72 0.5 16 ≤1 ≥4, in ...
Klebsiella pneumoniae is spread through exposure to the Klebsiella bacteria from an infected person, according to the U.S. Centers for Disease Control and Prevention. Exposure typically occurs by way...
The increasing speed and decreasing cost of high-throughput DNA sequencing technologies are enabling its application to the practice of medicine (35). Here, we tested whether genome sequencing could help to unravel a nosocomial outbreak and affect hospital infection control decisions. We sequenced patient and environmental isolates within a clinically relevant turnaround time during a hospital KPC-K. pneumoniae outbreak. Among the key insights provided by sequencing were that (i) the outbreak was monoclonal, despite a 3-week interval between the index case and the identification of subsequent cases, (ii) transmission likely occurred from at least two different sites of the index patient, (iii) at least three independent transmission events from the index patient led to two major clusters of colonized patients, (iv) one patient could be linked to a contaminated ventilator, and (v) a small number of putative resistance mutations could be identified in newly colistin-resistant isolates. Moreover, ...
In the last decade, a new hypervirulent (hypermucoviscous) variant of Klebsiella pneumoniae has been described (1). Most isolates of hypervirulent K. pneumoniae are very susceptible to antimicrobials (except ampicillin). However, a multidrug-resistant and hypervirulent variant of K. pneumoniae has also been described as the next "superbug" (2). On the other hand, Klebsiella variicola is a Gram-negative rod of the Enterobacteriaceae family; it was described as a new bacterial species in 2004 (3). Currently, K. variicola is known to be an endophyte of plants (3, 4), a symbiont in insects (5), and a pathogen in humans (3). A susceptible and multiresistant phenotype of K. variicola has been identified, corresponding to an extended spectrum β-lactamase (ESBL)-producing K. variicola, encoding the SHV-type and CTX-M-15 genes (6, 7).. It is difficult to distinguish K. variicola from K. pneumoniae biochemically as bacterial species. Therefore, it is necessary to use molecular tools such as the rpoB ...
Phenotypic and Genotypic Characterization of Extended Spectrum β-Lactamase Klebsiella pneumoniae and Fluorescent Pseudomonas spp. Strains from Market Garden Products and Their Watering Water in Benin West Africa. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Seventeen genes specifically required for nitrogen fixation are clustered on the chromosome of Klebsiella pneumoniae and form a complex regulon that is organized into eight transcriptional units. The nif promoters are representative of a new class of promoter, the members of which lack the consensus sequences normally found in prokaryotic promoters, nif gene transcription is positively controlled and requires: (1) the ntrA gene product, which replaces the rpoD-encoded sigma subunit of RNA polymerase to allow recognition of nif promoter sequences; and (2) the product of either the nitrogen regulation gene ntrC or the specific nif regulatory gene, nifA, which are both transcriptional activators. Most nif promoters require an upstream activator sequence (UAS) for nifA-mediated activation. The UAS acts independently of orientation and can function when placed 2 kilobases upstream from the transcription start site. Current evidence suggests that activation requires an interaction between proteins ...
TY - JOUR. T1 - Effect of piperacillin-tazobactam vs meropenem on 30-day mortality for patients with e coli or Klebsiella pneumoniae bloodstream infection and ceftriaxone resistance. AU - Harris, Patrick N.A.. AU - Tambyah, Paul A.. AU - Lye, David C.. AU - Mo, Yin. AU - Lee, Tau H.. AU - Yilmaz, Mesut. AU - Alenazi, Thamer H.. AU - Arabi, Yaseen. AU - Falcone, Marco. AU - Bassetti, Matteo. AU - Righi, Elda. AU - Rogers, Benjamin A.. AU - Kanj, Souha. AU - Bhally, Hasan. AU - Iredell, Jon. AU - Mendelson, Marc. AU - Boyles, Tom H.. AU - Looke, David. AU - Miyakis, Spiros. AU - Walls, Genevieve. AU - Al Khamis, Mohammed. AU - Zikri, Ahmed. AU - Crowe, Amy. AU - Ingram, Paul. AU - Daneman, Nick. AU - Griffin, Paul. AU - Athan, Eugene. AU - Lorenc, Penelope. AU - Baker, Peter. AU - Roberts, Leah. AU - Beatson, Scott A.. AU - Peleg, Anton Y.. AU - Harris-Brown, Tiffany. AU - Paterson, David L.. AU - for the MERINO Trial Investigators and the Australasian Society for Infectious Disease Clinical ...
Gene target information for blaNDM-1 - NDM_carbapenemase (Klebsiella pneumoniae). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Recent trials demonstrate increased pneumonia risk in chronic obstructive pulmonary disease patients treated with the inhaled corticosteroid (ICS) fluticasone propionate (FP). There is limited work describing FP effects on host defenses against bacterial pneumonia. C57BL/6 mice received daily, nose-only exposure to nebulized FP or vehicle for 8 days, followed by pulmonary challenge with Klebsiella pneumoniae. Bacterial burden, phagocytosis, leukocyte recruitment, cytokine expression, nitric oxide release, and survival were measured. Inhaled FP increased bacterial burden in lungs and blood 48 h after infection but affected neither in vivo phagocytosis of bacteria by alveolar macrophages (AM) nor alveolar neutrophil recruitment. AM from FP-treated mice showed impaired expression of infection induced TNF-alpha, IP-10 (CXCL-10), and interleukin 6 (IL-6), and AM also showed a trend towards impaired intracellular pathogen control following in vivo infection. In vitro FP treatment resulted in a dose-dependent
The objective of this study was to correlate resistance mutations of extended spectrum beta-lactamases (ESBL) and AmpC beta-lactamases and virulence factors (VF) with 30-day mortality in patients treated with either piperacillin-tazobactam or carbapenems. A post-hoc analysis on 123 patients with ceftriaxone-resistant Escherichia coli and Klebsiella pneumoniae bacteremia treated empirically with piperacillin-tazobactam and carbapenems was performed. Beta-lactamase resistance mutations and VF were identified by whole genome sequencing (WGS). The primary endpoint was 30-day mortality. Multivariate analyses were performed using logistic regression. WGS showed diverse multilocus sequence types (MLST) in 43 K. pneumoniae strains, while ST131 predominated in E. coli strains (57/80). CTX-M was most commonly detected (76/80 [95%] of E. coli; 39/43 [91%] of K pneumoniae.), followed by OXA (53/80 [66%] of E. coli; 34/43 [79%] of K. pneumoniae). A significant correlation was found between the number of ...
Just, H.M., Carneiro Leao, M.T.R., Daschner, F.D. und Andreesen, Reinhard (1987) Antibacterial activity of monocytes and macrophages against Staphylococcus aureus and Klebsiella pneumoniae in combination with Latamoxef. In: Szentiavanyi, A. und Friedman, H. und Gillissen, G., (eds.) Antibiosis and Host Immunity. Plenum Press, New York, S. 203-210. ISBN 0306426005; 978-0306426001. Im Publikationsserver gibt es leider keinen Volltext zu diesem Eintrag. ...
PubMed journal article: A coup détat by NDM-producing Klebsiella pneumoniae overthrows the major bacterial population during KPC-directed therapy. Download Prime PubMed App to iPhone, iPad, or Android
ABSTRACT The present study was investigated the immunological effects of the lipopolysaccharide extracted from Klebsiella pneumoniae against experimental infection with toxoplasmosis in mice. Immunological changes in treated mice were compared with the +ve (mice infected with T.gondii but not treated with LPS) and -ve (mice not infected with T.gondii and not treated with LPS) group at 3,14 and 30 days post infection with tissue cysts of T.gondii in the peritoneal cavity. Total and differential WBC count of peripheral blood and innate immune response represented by the phagocytic index were the criteria taken into consideration. Results showed a significant increase in the total WBC count in mice treated with LPS after infection with T.gondii, compared with -ve and +ve control groups. For differential WBC count, an increase in lymphocytes and decrease in monocytes, neutrophils and eosinophils was noticed. Basophils were not considered because of their low number.For phagocytic index, an increase was
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Identifying transmission route of antimicrobial-resistant pathogen is essential for appropriate infection control strategy in healthcare facilities. We report the utility of single-nucleotide...
This study shows the effect of exposure of mice to an altitude of 35,000 feet and to an atmosphere consisting of approximately 85% oxygen, 10% carbon dioxide, and 5% nitrogen for 3, 7, 14, and 30 days on susceptibility to respiratory infection caused by aerosols of Klebsiella pneumoniae. Mice exposed to 35,000 feet for 14 days prior to challenge and then returned to 35,000 feet exhibited an increase in mortality from 37% to 76%. Mice exposed to 35,000 feet for 14 days prior to challenge and then kept at ambient altitude exhibited an increase in mortality from 37% to 53%.There was an increased neutrophilic percentage accompanied with a lower white cell count in the blood of mice exposed to 35,000 feet for 14 days. Mice lost weight initially when placed at 35,000 feet. They started gaining weight after about 2 weeks, and the rate of weight gain was approximately that of mice kept at ambient altitude. Mice kept at 35,000 feet consumed more food than mice kept at ambient altitude. (Author)(*SPACE
Audebert, A.; Duflo, B.; Schaeffer, A.; Pequignot, H., 1973: A series of 14 cases of Klebsiella pneumoniae septicemia observed in a general medical unit
The Klebsiella pneumoniae database has moved to the Pasteur MLST site (http://www.pasteur.fr/mlst/). Please update your bookmarks.. ...
Characterization of NLRP12 during the In Vivo Host Immune Response to Klebsiella pneumoniae and Mycobacterium tuberculosis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Kısa Bildiri/Short Communication Mikrobiyol Bul 2012; 46(1): Yenidoğan Yoğun Bakım Ünitesinde Ortaya Çıkan GSBL Pozitif Klebsiella pneumoniae Nedenli…
Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The cotton is also resistant to lepidopteran insects through the introduction of cry1Ac from Bacillus thuringiensis subsp. Kurstaki ...
Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The cotton is also resistant to lepidopteran insects through the introduction of cry1Ac from Bacillus thuringiensis subsp. Kurstaki ...
Arifun Nahar gave birth to her 3rd child on December 16 at a private hospital in Dhaka but her baby boy fell sick soon after and eventually died 28 days after birth. The cause of his sudden death was an otherwise harmless bacterium -- Klebsiella pneumoniae -- that turned deadly, possibly in hospital, by being resistant to 15 types of antibiotics. ...
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The pilot grants, which award researchers up to $50,000, support up to one year of research.. The awards are provided through GEM, an interdisciplinary community of UB faculty and staff dedicated to advancing research on the genome and microbiome. GEM is one of UBs three Communities of Excellence, a $9 million initiative to harness the strengths of faculty and staff from fields across the university to confront the challenges facing humankind through research, education and engagement.. Along with Surtees and Nowak, GEM is led by executive director Timothy F. Murphy, MD, SUNY Distinguished Professor of medicine and senior associate dean for clinical and translational research.. In the second round of research pilots, funding was given to projects that are studying a lethal form of the bacterium Klebsiella pneumoniae, investigating the role that skin plays in our vulnerability to autoimmune disorders and examining pathogenic fungi that cause serious illness among those with weakened immune ...
Carbapenems are antibiotics of last-resort. These agents are crucial for preventing and treating life-threatening bacterial infections. Carbapenemase enzymes, which degrade carbapenems thereby conferring carbapenem resistance, are harbored on transmissible mobile genetic elements called plasmids that are easily spread from species to species and even among different genera of Gram-negative bacteria. Gram-negative bacteria harboring carbapenemase enzymes, in particular Klebsiella pneumoniae carbapenemases (KPC), have been identified in nearly all States in the U.S. Even more concerning is the increasing reports of the appearance of non-endemic carbapenemase variants in the U.S. such as New Delhi metallo-β-lactamase (NDM)-producing Gram-negative bacilli. Early detection of CPOs in the health care-setting is required as patients with unrecognized colonization with a CPO serve as a reservoir for transmission during health-care associated outbreaks. Therapeutic options for infections caused by a CPO ...
The emergence and global spread of carbapenemase-producing Enterobacteriaceae is of great concern to health services worldwide. These bacteria are often resistant to all beta-lactam antibiotics and frequently co-resistant to most other antibiotics, leaving very few treatment options. The epidemiology is compounded by the diversity of carbapenem-hydrolysing enzymes and the ability of their genes to spread between different bacterial species. Difficulties are also encountered by laboratories when trying to detect carbapenemase production during routine diagnostic procedures due to an often heterogeneous expression of resistance. Some of the resistance genes are associated with successful clonal lineages which have a selective advantage in those hospitals where antimicrobial use is high and opportunities for transmission exist; others are more often associated with transmissible plasmids. A genetically distinct strain of Klebsiella pneumoniae sequence type (ST) 258 harbouring the K. pneumoniae
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The Klebsiella pneumoniae carbapenemase (KPC) was detected in a carbapenem-resistant respiratory isolate of Klebsiella pneumoniae in an Irish hospital. This is the first report of a KPC-producing isolate in the Republic of Ireland. The isolate was resistant to all β-lactams. Furthermore, it had reduced susceptibility to three other classes of non-β-lactam antibiotics. The isolate was not associated with travel abroad. Detection of KPC-producing bacteria has important infection control and public health implications.
Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are a group of emerging highly drug-resistant Gram-negative bacilli causing infections associated with significant morbidity and mortality. Once confined to outbreaks in the northeastern United States (US), they have spread throughout the US and most of the world. KPCs are an important mechanism of resistance for an increasingly wide range of Gram-negative bacteria and are no longer limited to K pneumoniae. KPC-producing bacteria are often misidentified by routine microbiological susceptibility testing and incorrectly reported as sensitive to carbapenems; however, resistance to the carbapenem antibiotic ertapenem is common and a better indicator of the presence of KPCs. Carbapenem antibiotics are generally not effective against KPC-producing organisms. The best therapeutic approach to KPC-producing organisms has yet to be defined; however, common treatments based on in vitro susceptibility testing are the polymyxins, tigecycline, and ...
Download Free Full-Text of an article RAPID DETECTION OF EXTENDED SPECTRUM ?-LACTAMASES (ESBLS) PRODUCING ISOLATES OF KLEBSIELLA PNEUMONIA BY A NEW COLORIMETRIC MEDIUM
Germany has reported an outbreak of carbapenemase-producing (NDM-1 and OXA-48) and colistin-resistant Klebsiella pneumoniae sequence type (ST) 307. As of 21 October 2019, 17 patients in three hospitals and one rehabilitation clinic in Mecklenburg-West Pomerania in north-east Germany have been affected. Six of the 17 cases presented with clinical symptoms of infection, while 11 were identified as be carriers. ...
Germany has reported an outbreak of carbapenemase-producing (NDM-1 and OXA-48) and colistin-resistant Klebsiella pneumoniae sequence type (ST) 307. As of 21 October 2019, 17 patients in three hospitals and one rehabilitation clinic in Mecklenburg-West Pomerania in north-east Germany have been affected. Six of the 17 cases presented with clinical symptoms of infection, while 11 were identified as be carriers. ...
TY - JOUR. T1 - 1-(5-acetamido-2-hydroxyphenyl)-3-propan-1-ones. T2 - Synthesis, antioxidant and antimicrobial activities. AU - Shobha, K. L.. AU - Simon, Lalitha. AU - Rao, Amita S.. AU - Ganesh Maiya, B. C.. AU - Srinivasan, K. K.. PY - 2016. Y1 - 2016. N2 - A series of 1-(5-acetamido-2-hydroxyphenyl)-3-propan-1-one derivatives were synthesized and evaluated for their antioxidant activity by ABTS radical scavenging assay. Compounds with -OH attached to C-2 of ringB (D6 ) and - OH and -OCH3 attached to C-4 and C-3 respectively of the ringB(D4 ) were found to be more potent than the standard used. The antibacterial activity of synthesized compounds was investigated against the bacterial strains Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae and antifungal activity against Candida albicans using agar well diffusion method. All the compounds showed good inhibition against Klebsiella pneumoniae at 12.5μg /mL concentration.CompoundsD1 and D10displayed ...
In neonates with sepsis testing of K. pneumoniae isolates for ESBL production was positive in 60 percent of cases, in neonates with UTI -- in 40 percent of cases. The authors commented that one of key virulence factors -- the rmpA gene -- was found in both groups of infants. This means the prevalence of virulent K. pneumoniae strains is higher than was previously thought, and heavier clinical forms of diseases were found in patients with those virulent strains.
Intervention and Testing. Conjunctival swabs and corneal scrapings from the right eye were inoculated for culture. The isolate was analyzed for the presence of the mucoid phenotype and the ability to form biofilm. We also investigated whether the formation of biofilm by the corneal Klebsiella isolate is affected by N-acetylcysteine ...
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