TY - JOUR. T1 - Predictors of Mortality in Bloodstream Infections Caused by Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae: Importance of Combination Therapy. AU - Tumbarello, Mario. AU - Viale, P. AU - Viscoli, C. AU - Trecarichi, Em. AU - Tumietto, F. AU - Marchese, A. AU - Spanu, T. AU - Ambretti, S. AU - Ginocchio, F. AU - Cristini, F. AU - Losito, Ar. AU - Tedeschi, S. AU - Cauda, R. AU - Bassetti, M.. PY - 2012. Y1 - 2012. N2 - Background. The spread of Klebsiella pneumoniae (Kp) strains that produce K. pneumoniae carbapenemases (KPCs) has become a significant problem, and treatment of infections caused by these pathogens is a major challenge for clinicians.Methods. In this multicenter retrospective cohort study, conducted in 3 large Italian teaching hospitals, we examined 125 patients with bloodstream infections (BSIs) caused by KPC-producing Kp isolates (KPC-Kp) diagnosed between 1 January 2010 and 30 June 2011. The outcome measured was death within 30 days of the first ...

antibiotic resistance markers and future transformation selection technologies. Organisms resistant to ampicillin by virtue of. Klebsiella pneumoniae.. nitrofurantoin (66%) but less susceptible to ampicillin (4%) and ticarcillin (7%). but very resistant to. Klebsiella pneumoniae ATCC 700603 and Escherichia.Klebsiella oxytoca; Klebsiella pneumoniae; Legionella; Morganella morganii; Proteus rettgeri; Proteus vulgaris; Providencia; Pseudomonas; Serratia; Yersinia.Why is klebsiella resistant to ampicillin ampicillin hcpcs code ampicillin plus tobramycin order ampicillin bertibarots ampicillin droge.réservez vos places Lanfains 22 - BZH - France (près de St-Brieuc) Accueil Infos pratiques éditions passées partenaires Lorganisation Presse Contact.Ampicilline + gentamicine. les germes plus inhabituels tels que la Klebsiella pneumoniae,. Carr TL, Beazley DD et al. Antibiotic use in pregnancy and drug.Ampicilline G (1962) Ent robact ries (1964) C phalosporines 3G (1980) Ent robact. 2/ Klebsiella ...

Multi-drug resistant Klebsiella pneumoniae strains are a common cause of health care associated infections worldwide. Clonal spread of Klebsiella pneumoniae isolates carrying plasmid mediated CTX-M-15 have been commonly reported. Limited data is available regarding dissemination of chromosomally encoded CTX-M-15 in Klebsiella pneumoniae worldwide. We examined 23 non-repetitive ESBL-producing Klebsiella pneumoniae strains isolated from clinical specimens over a period of 4 months in a German University Hospital. All isolates were characterized to determine their genetic relatedness using Pulsed-Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST). PFGE revealed three clusters (B1, B2, and B3) with a sub-cluster (A3) comprising of 10 isolates with an identical PFGE pattern. All strains of the cluster B3 with similar PFGE patterns were typed as ST101, indicating an outbreak situation. The ESBL allele bla CTX-M-15 was identified in 16 (69.6 %) of all isolates, including

TY - JOUR. T1 - Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. AU - Kim, Mi Na. AU - Yong, Dongeun. AU - An, Dongheui. AU - Chung, Hae Sun. AU - Woo, Jun Hee. AU - Lee, Kyungwon. AU - Chong, Yunsop. PY - 2012/4/1. Y1 - 2012/4/1. N2 - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four patients with no history of traveling abroad who stayed for 21 to 205 days in a tertiary care hospital. All were sequence type (ST) 340 and had nearly identical XbaI pulsed-field gel electrophoresis (PFGE) patterns. The bla NDM-1-carrying plasmids were in the IncN group, with sizes ranging from 50 to 200 kb. These findings suggest that NDMKP had already been introduced into South Korea before this clustering was found.. AB - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four ...

The pipeline guide provides a snapshot of the global therapeutic landscape of Klebsiella Pneumoniae Infections. The pipeline guide reviews pipeline therapeutics for Klebsiella Pneumoniae Infections by companies and universities/research institutes based on information derived from company and industry-specific sources.

It appears as a mucoid lactose fermenter on MacConkey agar. Klebsiella pneumonia ( KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. B) A total of 5, 475 samples collected from children resulted in laboratory- positive cultures; the 5 most frequently occurring bacterial species accounted for [ approximately equal to] 70% of total bacterial infections, and Klebsiella pneumoniae ( white bar) was the third most dominant ( 710 isolates). Klebsiella pneumoniae is a bacterium that causes UTIs. Pneumoniae is the most prevalent and clinically important. For example, Klebsiella must enter the respiratory ( breathing) tract to cause pneumoniae, or the blood to cause a bloodstream infection. Klebsiella pneumoniae și articulaţii. To get a Klebsiella infection, a person must be exposed to the bacteria. The organism resides in the upper respiratory tract and gastrointestinal tract of healthy individuals. Klebsiella pneumoniae is a bacterial organism that is responsible for causing ...

TY - JOUR. T1 - An outbreak of KPC-Producing klebsiella pneumoniae linked with an index case of community-acquired KPC-producing isolate. T2 - Epidemiological investigation and whole genome sequencing analysis. AU - Song, Je Eun. AU - Jeong, Haeyoung. AU - Lim, Young Sun. AU - Ha, Eun Jin. AU - Jung, In Young. AU - Jeong, Wooyong. AU - Choi, Heun. AU - Jeong, Su Jin. AU - Ku, Nam Su. AU - Park, Eun Suk. AU - Yong, Dongeun. AU - Lee, Kyungwon. AU - Kim, June Myung. AU - Choi, Jun Yong. N1 - Publisher Copyright: © Copyright 2019, Mary Ann Liebert, Inc., publishers.. PY - 2019/12. Y1 - 2019/12. N2 - Aims: A hospital outbreak of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPN) linked with an index case of community-acquired infection occurred in an urban tertiary care hospital in Seoul, South Korea. Therefore, we performed an outbreak investigation and whole genome sequencing (WGS) analysis to trace the outbreak and investigate the molecular characteristics of the ...

klebsiella. FAQ. Medical Information. Klebsiella pneumoniae; Klebsiella oxytoca; Enterobacteriaceae; Enterobacter;. Ciprofloxacin; HLA-B27 Antigen; Aminoglycosides.. Characterization of an IncN2-type bla NDM-1-carrying plasmid in Escherichia coli ST131 and Klebsiella pneumoniae ST11 and ST15 isolates in Thailand.ColonizaciÓn fecal por cepas de Klebsiella pneumoniae productoras de betalactamasas de espectro. and more than 90% were also susceptible to ciprofloxacin and.Community-acquired Klebsiella pneumoniae meningitis in an alcoholic patient with an infected pancreatic pseudocyst; a case report and review of literature.%T Urinary pharmacodynamics of low-dose ciprofloxacin and ofloxacin %A STEIN G. E. %A SCHOOLEY S. MIC = 4; Klebsiella pneumoniae, MIC = 4; Staphylococcus.Titre du document / Document title Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type β-lactamase-producing.Titre du document / Document title Extended-spectrum beta-lactamases ...

TY - JOUR. T1 - Prevention of colonization and infection by klebsiella pneumoniae carbapenemase-producing enterobacteriaceae in long-term acute-care hospitals. AU - Hayden, Mary K.. AU - Lin, Michael Y.. AU - Lolans, Karen. AU - Weiner, Shayna. AU - Blom, Donald. AU - Moore, Nicholas M.. AU - Fogg, Louis. AU - Henry, David. AU - Lyles, Rosie. AU - Thurlow, Caroline. AU - Sikka, Monica. AU - Hines, David. AU - Weinstein, Robert A.. PY - 2015/4/15. Y1 - 2015/4/15. N2 - Background. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (hereafter KPC) are an increasing threat to healthcare institutions. Long-term acute-care hospitals (LTACHs) have especially high prevalence of KPC. Methods. Using a stepped-wedge design, we tested whether a bundled intervention (screening patients for KPC rectal colonization upon admission and every other week; contact isolation and geographic separation of KPC-positive patients in ward cohorts or single rooms; bathing all patients daily with ...

Bacteriophages are a promising alternative for curtailing infections caused by multi drug resistant (MDR) bacteria. The objective of the present study is to evaluate phage populations from water bodies to inhibit planktonic and biofilm mode of growth of drug resistant Klebsiella pneumoniae...

Abstract. Klebsiella pneumoniae is a gram-negative rod enterobacteria that is notorious for its role as carrier of extended spectrum beta-lactamase (ESBL) and its carbapenem resistant Enterobacteriaceae (CRE) species. However, hypermucoviscous Klebsiella pneumoniae is distinctly different from ESBL and CRE. We report a case of neck necrotizing fasciitis caused by hypermucoviscous Klebsiella pneumoniae in a 56-year-old male who presented to our emergency department (ED) with a swollen neck. His condition deteriorated rapidly requiring emergency intubation to secure his airway. Despite aggressive resuscitation and treatment with broad spectrum antibiotics and cytokine adsorption therapy, the patient succumbed to his disease. This report describes the clinical characteristics of hypermucoviscous Klebsiella pneumoniae and emphasizes the importance of early detection and subsequent aggressive source control interventions in necrotizing fascitiis caused by this particular bacteria.. ...

TY - JOUR. T1 - Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice. AU - Ali, Mehboob. AU - Umstead, Todd M.. AU - Haque, Rizwanul. AU - Mikerov, Anatoly N.. AU - Freeman, Willard M.. AU - Floros, Joanna. AU - Phelps, David. PY - 2010/6/17. Y1 - 2010/6/17. N2 - Background: Surfactant protein-A (SP-A) has been shown to play a variety of roles related to lung host defense function. Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with Klebsiella pneumoniae by 2D-DIGE.Methods: Mice were infected intratracheally with K. pneumoniae and after 4 and 24 hours they were subject to BAL. Cell-free BAL was analyzed by 2D-DIGE on two-dimensional gels with pH ranges of 4-7 and 7-11. Under baseline conditions and at 4 and 24 hr post-infection BAL was compared between untreated and infected wild type and SP-A-/- mice. Sixty proteins ...

Abstract. We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14.. Keywords: Klebsiella pneumoniae Carbapenemase (KPC), multidrug resistance, whole genome sequencing ...

The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and the human host. Sequence analysis revealed novel plasmid-located virulence factors, including a cluster for glycogen synthesis. Glycogen production is considered to be one of the possible adaptive responses to long-term survival and growth in environments outside the host. Chromosomally-encoded virulence traits in the clone

Klebsiella pneumonia (KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. It is typically due to aspiration and alcoholism may be a risk factor, though it is also commonly implicated in hospital-acquired urinary tract infections, and COPD(chronic obstructive pulmonary disease) individuals Individuals with Klebsiella pneumonia tend to cough up a characteristic sputum, as well as having fever, nausea, tachycardia and vomiting. Klebsiella pneumonia tends to affect people with underlying conditions, such as alcoholism. The cause of the condition Klebsiella pneumonia is Klebsiella pneumoniae which is gram-negative, as well as rod-shaped, glucose-fermenting, facultative anaerobic bacterium. In terms of the pathophysiology of Klebsiella pneumonia we see neutrophil myeloperoxidase defense against K P.Oxidative inactivation of elastase is involved, while LBP helps transfer bacteria cell wall elements to the cells. Klebsiella resistant strains have been recorded in USA with a ...

TY - CONF. T1 - Successful treatment of KPC-3 Klebsiella Pneumoniae ST258 clone with a combination of high-dose tigecycline and colistin in ICU: a case series report.. AU - Gulotta, Gaspare. AU - Raineri, Santi Maurizio. AU - Bonura, Celestino. AU - Pantuso, Gianni. AU - Cortegiani, Andrea. AU - Giarratano, Antonino. AU - Agrusa, Antonino. AU - Di Carlo, Paola. AU - Cocorullo, Gianfranco. AU - Giammanco, Anna. AU - Mammina, Caterina. PY - 2011. Y1 - 2011. N2 - INFECTIONS CAUSED BY KLEBSIELLA PNEUMONIAE SEQUENCE TYPE 258 PRODUCING K. PNEUMONIAE CARBAPENEMASE 3 (KPC-Kp)HAVE WIDELY EMERGED AND BOTH INDIVIDUAL CASES AND OUTBREAKS OF COLONIZATION OR INFECTION HAVE BEEN REPORTED IN PALERMO, ITALY.OBIETTIVO: THIS IS A RETROSPECTIVE CASE SERIES THAT DESCRIBES THE CLINICAL AND MICROBIOLOGIC OUTCOMES OF 16 PATIENTS WHO RECEIVED A COMBINATION OF HIGH-DOSE TIGECYCLINE AND COLISTIN FOR TREATMENT OF VAP (4 CASES) AND SEVERE BACTERAEMIA (12 CASES) DURING THE YEARS 2009-2011. 11 OUT OF THE 16 CASES WERE POST ...

Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies. Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages. Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against 254 clinical Enterobacteriaceae strains including multidrug resistant Klebsiella isolates producing extended-spectrum beta-lactamases (ESBLs). Based on their lytic potential, six of the phages were further characterised for burst size, physicochemical properties and sensitivity to

Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies. Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages. Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against 254 clinical Enterobacteriaceae strains including multidrug resistant Klebsiella isolates producing extended-spectrum beta-lactamases (ESBLs). Based on their lytic potential, six of the phages were further characterised for burst size, physicochemical properties and sensitivity to

The global emergence of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) multilocus sequence type ST258 is widely recognized. Less is known about the molecular and epidemiological details of non-ST258 K. pneumoniae in the setting of an outbreak mediated by an endemic plasmid. We describe the interplay of blaKPC plasmids and K. pneumoniae strains and their relationship to the location of acquisition in a U.S. health care institution. Whole-genome sequencing (WGS) analysis was applied to KPC-Kp clinical isolates collected from a single institution over 5 years following the introduction of blaKPC in August 2007, as well as two plasmid transformants. KPC-Kp from 37 patients yielded 16 distinct sequence types (STs). Two novel conjugative blaKPC plasmids (pKPC_UVA01 and pKPC_UVA02), carried by the hospital index case, accounted for the presence of blaKPC in 21/37 (57%) subsequent cases. Thirteen (35%) isolates represented an emergent lineage, ST941, which contained pKPC_UVA01 in 5/13 (38%

Klebsiella pneumoniae ATCC ® BAA-1705D-5™ Designation: Genomic DNA from Klebsiella pneumoniae strain ART 2008133 TypeStrain=False Application: Source culture is Modified Hodge Test (MHT) positive control designation Source culture produces Klebsiella pneumoniae carbapenemase (KPC)

Klebsiella Pneumoniae Infections - Market Insights, Epidemiology and Market Forecast - 2025 is a market research report available at US $5750 for a Single User PDF License from RnR Market Research Reports Library.

Background and Objective: The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae. The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic methods. Method: Out of 800 strains, 270 K. pneumoniae strains (33.7%), were obtained. Antibiotic susceptibility test was performed by disk diffusion method in accordance with CLSI guidelines. Carbapenem resistant strains were identified by the Modified Hodge Test based on CLSI instruction and PCR for surveying the presence of bla-KPC gene. Results: A total 270 K. pneumoniae strains were collected. Antibiotic susceptibility test results showed the highest and lowest resistance was related to piperacillin (60.6%) and carbapenems (14.6%) respectively. 80.5% (33 of 41) isolates were positive by MHT, but all of them (100%) were negative for amplification of the bla-KPC

To the Editor: Until a few years ago, the most frequent microbiologically documented cause of severe bloodstream infections among patients with hematologic malignancies was gram-positive bacteria (1). However, over the years, gram-negative bacteria have become the main infectious cause of death among patients with hematologic malignancies, and rates of different phenotypes associated with antimicrobial drug resistance are increasing (2). This trend could be the result of increasing empirical use of antimicrobial drug therapy and prophylaxis and use of new, more effective antimicrobial drugs. In particular, over the past few years at our hospital (Agostino Gemelli Teaching Hospital, Rome, Italy), we have observed a progressive increase in bloodstream infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp), which are responsible for a dramatic new scenario.. We reviewed records of all patients affected by hematologic malignancies who were admitted to the hospital ...

TY - JOUR. T1 - Phenotypical and molecular assessment of the virulence potential of KPC-3-producing Klebsiella pneumoniae ST392 clinical isolates. AU - DApolito, Danilo. AU - Arena, Fabio. AU - Conte, Viola. AU - De Angelis, Lucia Henrici. AU - Di Mento, Giuseppina. AU - Carreca, Anna Paola. AU - Cuscino, Nicola. AU - Russelli, Giovanna. AU - Iannolo, Gioacchin. AU - Barbera, Floriana. AU - Pasqua, Salvatore. AU - Monaco, Francesco. AU - Cardinale, Francesca. AU - Rossolini, Gian Maria. AU - Conaldi, Pier Giulio. AU - Douradinha, Bruno. N1 - Copyright © 2020 Elsevier GmbH. All rights reserved.. PY - 2020/11. Y1 - 2020/11. N2 - Klebsiella pneumoniae is a Gram-negative bacterium of clinical importance, due to its resistance to several antibiotic classes. We have identified 4 clinical isolates of K. pneumoniae sequence type (ST) 392 KPC-3-producing strains from patients at the Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione (IRCCS-ISMETT), a Southern Italian ...

Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence ...

Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Citrobacter freundii at a Tertiary Acute Care Facility in Miami, Florida - Volume 38 Issue 3

Carbapenemase-producing Enterobacteriaceae (CPE) have emerged in recent years as being among of the most important threats to public health. CPE have been detected in almost all European countries, although with a highly variable geographical distribution (1). Some high-risk clones of Klebsiella pneumoniae, mainly KPC-producing sequence type 258 (ST258) but also OXA-48-producing ST395 or ST15, have been implicated in the worldwide spread of carbapenemases (2-4).. Previous studies have shown that the population of Escherichia coli strains resistant to amoxicillin-clavulanic acid is less diverse than the susceptible population (5). Little is known about the population structure of carbapenemase-producing K. pneumoniae in comparison with the carbapenem-susceptible population. The aim of this study was to test the hypothesis that the carbapenemase-producing K. pneumoniae strains isolated in Spanish hospitals constitute a distinct and more homogeneous population than the carbapenemase-susceptible ...

Researchers at Queens University Belfast together with the University of Vienna have discovered that treatment for the antibiotic resistant bacteria Klebsiella pneumoniae could lie within our bodies natural defences. Multidrug resistance of microbes poses a serious global threat to human health. Globally, 700,000 people die every year due to antimicrobial resistance. The bacteria Klebsiella pneumoniae causes a number of infections including sepsis, urinary tract infections and pneumonia. As Klebsiella becomes more resistant to antibiotics, these common infections are becoming increasingly difficult to treat, which has led to the World Health Organisation recently declaring an urgent need for new therapeutics to be discovered for Klebsiella.. Professor Jose Bengoechea from the Wellcome-Wolfson Institute for Experimental Medicine at Queens University Belfast and one of the lead researchers explains: Klebsiella pneumoniae is of particular concern as it can cause infections such as bladder ...

Serious infections in intensive care unit patients caused by multidrug-resistant (MDR) Klebsiella pneumoniae represent a major threat worldwide owing to increased mortality and limited treatment options. With the application of tigecycline for MDR pathogens, tigecycline-non-susceptible K. pneumoniae isolates have recently emerged in China. To identify the susceptibility profile of MDR K. pneumoniae to tigecycline and evaluate the molecular characterization of tigecycline resistance, 214 MDR K. pneumoniae isolates were collected from blood samples of patients in intensive care units. MICs and clonal relatedness were determined by standard broth microdilution and multilocus sequence typing, respectively. Expression levels of efflux pumps and their global regulators were examined using real-time PCR. Mutations of local repressor were identified by PCR and sequencing. Our results show that the tigecycline resistance rate of 214 MDR K. pneumoniae isolates was 6.07 %. ST11 was the predominant clone type of

Background: Carbapenemase-producing Enterobacteriaceae (CPE), including KPC-producing Klebsiella pneumoniae (KPC-Kpn), are an increasing threat to patient safety. Objectives: To use WGS to investigate the extent and complexity of carbapenemase gene dissemination in a controlled KPC outbreak. Materials and methods: Enterobacteriaceae with reduced ertapenem susceptibility recovered from rectal screening swabs/clinical samples, during a 3 month KPC outbreak (2013-14), were investigated for carbapenemase production, antimicrobial susceptibility, variable-number-tandem-repeat profile and WGS [short-read (Illumina), long-read (MinION)]. Short-read sequences were used for MLST and plasmid/Tn4401 fingerprinting, and long-read sequence assemblies for plasmid identification. Phylogenetic analysis used IQTree followed by ClonalFrameML, and outbreak transmission dynamics were inferred using SCOTTI. Results: Twenty patients harboured KPC-positive isolates (6 infected, 14 colonized), and 23 distinct KPC-producing

Background: Carbapenemase-producing Enterobacteriaceae (CPE), including KPC-producing Klebsiella pneumoniae (KPC-Kpn), are an increasing threat to patient safety. Objectives: To use WGS to investigate the extent and complexity of carbapenemase gene dissemination in a controlled KPC outbreak. Materials and methods: Enterobacteriaceae with reduced ertapenem susceptibility recovered from rectal screening swabs/clinical samples, during a 3 month KPC outbreak (2013-14), were investigated for carbapenemase production, antimicrobial susceptibility, variable-number-tandem-repeat profile and WGS [short-read (Illumina), long-read (MinION)]. Short-read sequences were used for MLST and plasmid/Tn4401 fingerprinting, and long-read sequence assemblies for plasmid identification. Phylogenetic analysis used IQTree followed by ClonalFrameML, and outbreak transmission dynamics were inferred using SCOTTI. Results: Twenty patients harboured KPC-positive isolates (6 infected, 14 colonized), and 23 distinct KPC-producing

BioAssay record AID 1085598 submitted by ChEMBL: Antibacterial activity against Klebsiella pneumoniae at 1 ug/ml after 24 hr by well diffusion method.

The nexus between resistance determinants, plasmid type, and clonality appears to play a crucial role in the dissemination and survival of carbapenem-resistant Klebsiella pneumoniae (CRKP). The incidence of infections involving CRKP in Saudi Arabia is increasing and there is a need for detailed molecular profiling of this pathogen for CRKP surveillance and control. The resistance determinants of 71 non-redundant CRKP isolates were investigated by polymerase chain reaction (PCR) and sequencing. Plasmid typing was performed using PCR-based replicon typing and the clonality of isolates was determined by multilocus sequence typing. Capsular polysaccharide synthesis genes and other virulence factors were examined using multiplex PCR. Diversity was calculated using DIVEIN, clonal relationship was determined using eBURST, and phylogenetic analysis was performed using SplitsTree4. A polyclonal OXA-48 gene alone was the most common carbapenemase detected in 48/71 (67.6%) isolates followed by NDM-1 alone in 9/71

Background. During 2006, Israeli hospitals faced a clonal outbreak of carbapenem-resistant Klebsiella pneumoniae that was not controlled by local measures. A nationwide intervention was launched to contain the outbreak and to introduce a strategy to control future dissemination of antibiotic-resistant bacteria in hospitals.. Methods. In March 2007, the Ministry of Health issued guidelines mandating physical separation of hospitalized carriers of carbapenem-resistant Enterobacteriaceae (CRE) and dedicated staffing and appointed a professional task force charged with containment. The task force paid site visits at acute-care hospitals, evaluated infection-control policies and laboratory methods, supervised adherence to the guidelines via daily census reports on carriers and their conditions of isolation, provided daily feedback on performance to hospital directors, and intervened additionally when necessary. The initial intervention period was 1 April 2007-31 May 2008. The primary outcome measure ...

External validation of the INCREMENT-CPE risk score (ICS) for 30-day all-cause mortality is needed. There is also scarce information about whether colistin resistance influences the prognosis of carbapenem-resistant Klebsiella pneumoniae (CRKp) bacteraemia. In this study, the ability of ICS to predict all-cause mortality in the KAPECOR cohort was calculated using the area under the receiver operating characteristic (AUROC) curve. The association of colistin resistance with mortality was studied. The ICS showed an AUROC curve of 0.77 (95% CI 0.68-0.86). A cut-off of 8 points showed 96.8% sensitivity and 50.7% specificity. Mortality of low-risk patients was not different in those treated with monotherapy versus combination therapy. However, mortality of high-risk patients treated with combination therapy (37.8%) was significantly lower than in those treated with monotherapy (68.4%) (P = 0.008). To study the prognostic significance of colistin resistance, 83 selected cases of bacteraemia due to ...

Results: Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum β-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates ...

Klebsiella pneumoniae is a frequent cause of infectious outbreaks in Neonatal Intensive Care Units (NICUs). The aim of this paper is to describe an outbreak occurred in a 13-bed NICU and the control measures adopted in order to interrupt the chain of transmission. We described the microbiological investigations, the NICU staff compliance to the infection control measures by means of a specifically designed check-list and the control measures adopted. Six cases of primary bloodstream infections sustained by ampicillin/piperacillin-resistant Klebsiella pneumoniae were observed over a two-month period. One culture obtained from a 12% saccarose multiple-dose solution allowed the growth of Klebsiella pneumoniae. During the inspections performed by the Hospital Infection Control Team, using the check-list for the evaluation of the NICU staff compliance to the infection control measures, several breaches in the infection control policy were identified and control measures were adopted. In our case the

Klebsiella pneumoniae is gram negative rod which present with large mucoid capsulre. The common virulence factors of klebsiella pneumonia may include protease, cell wall endotoxin and mucoid capsule.

Several bacterial populations areknownto be containing some fraction of cells which survive exposure to antibiotics and harsh environment, are called as persister cells. This fraction of cells is very small generally ranging from 10-7 to 10-5. The mechanism of persister formation is not yet clearly understood although expression of toxin-antitoxin (TA) pairs of proteins has been found to be associated with persister formation. Klebsiella pneumoniae is also shown to produce persister cells by prolonged exposure to ampicillin.In this study, we have identified a pair of proteins, hipA and hipB, of TA system in Klebsiella pneumoniae. The proteins have 70% and 60% sequence similarity respectively with their homologous proteins from E. coli. hipA and hipB associate together to regulate survival of persister cells by binding to DNA in unfavourable conditions. Both hipA and hipB proteins from Klebsiella pneumoniae were cloned, expressed and purified. The clones were over expressed in fusion with His-tag ...

Schwaber et al identified risk factors for acquisition of carbapenem-resistant Klebsiella pneumoniae in a hospitalized patient. These can help to identify a patient who should be screened for carriage. The authors are from Tel Aviv Sourasky Medical Center.

Introduction: The aim of this study was to investigate the presence of carbapenemase production and carbapenem resistance mechanisms in 47 carbapenem resistant Klebsiella pneumoniae isolates by phenotypic confirmatory tests and molecular assay.. Methodology: Carbapenem resistance genes KPC, OXA-48 and NDM were investigated with the BD MAX CRE assay kit in the BD MAX real time PCR instrument. Modified Hodge test, MBL gradient strip test, D70C Carbapenemase Detection Set, Temocillin gradient strip test methods were used as phenotypic confirmatory tests. Clonal relationship between study isolates was investigated with pulsed-field gel electrophoresis.. Results: Analysis with BD MAX CRE assay revealed OXA-48 positivity in 17 (36%) strains, NDM positivity in 6 (13%) strains and coexistence of OXA-48 + NDM positivity in 8 (17%) strains. In 16 (34%) strains, none of the KPC, OXA-48 and NDM genes were detected. While MHT was the most sensitive phenotypic confirmatory test, D70C disc set had not been ...

Reduced susceptibility to carbapenems in Gram-negative pathogens is an emerging feature of the antibiotic-resistance phenomenom Reports about strains resistant to this class of antibiotics among Enterobacteriaceae, particularly in Klebsiella pneumoniae, are increasing.The aims of this study were to assess the incidence of Klebsiella pneumoniae with reduced susceptibility to carbapenems in Bologna area and to carry out the characterization of these strains.The study included isolates of K. pneumoniae that showed reduced susceptibility to carbapenems, as detected by an automated system (Vitek2, bioMérieux). Between January and May 2009, 26 strains were collected (mainly isolated from urinary samples).These isolates were tested for susceptibility to carbapenems by E-test, to define MIC values for meropenem and ertapenem. Moreover, to detect the production of metallo-beta lactamases (MBL) and carbapenemases (KPC) were respectively performed the Etest with imipenem and imipenem/EDTA (IPM-IPM/EDTA) ...

Sequences of 11 amino acids belonging to the KPN_00363 protein and KPN_00459 protein from Klebsiella pneumoniae MGH 78578 which was previously identified as potentially immunogenic was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Overall design: 26 peptides, two representing the original sequences, 22 peptides with each residue replaced by alanine (or glycin, if the original sequence contained alanine) as well as two related peptides were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was seperated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interaction rabbit polyclonal IgG to K. pneumoniae (Abcam ab20947) was used, while non-specific binding to the epitope sequences was checked using rabbit polyclonal IgG to E. coli (Abcam ab137967) and S. enterica

Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely

Objective(s): Infections due to carbapenemase-producing Klebsiella pneumoniae are associated with high morbidity and mortality. In this study, we report a hospital outbreak due to co-producing OXA-48 and NDM-1 K. pneumoniae clone. The aim of the study is to investigate the clonal relationship of strains, risk factors of outbreak and infection control measures.Materials and Methods: Once an outbreak was suspected at the end of December 2017 in our intensive care unit (ICU), carbapenem resistance K. pneumoniae identified in patients specimens. An outbreak analysis was begun to determine the risk factors and dissemination of the cases. A case-control study was conducted to determine the risk factors. To control the outbreak; tight contact prevention, good clean-up the medical devices and hospital environment, were done. Staff training programs such as hand hygiene, disinfection, wearing aprons, good cleaning were created. Carbapenem resistance genes determined by PCR. Clonal relationships of strains

BACKGROUND:For abdominal solid organ transplant (ASOT) recipients, infection with Klebsiella pneumoniae, particularly carbapenem-resistant K. pneumoniae (CRKP), can be life-threatening. The aims of this study were to characterize the risk factors associated with acquisition of CRKP and 90-day crude mortality among patients. MATERIAL AND METHODS:In our cohort study, we retrospectively reviewed 68 K. pneumoniae-infected transplant recipients, studied their demographics, clinical manifestations, microbiology, and outcomes, and determined the risk factors associated with the occurrence of CRKP and crude mortality due to K. pneumoniae infections. RESULTS:Sixty-eight ASOT recipients (5.4%) experienced 78 episodes of K. pneumoniae infection. Among these, 20 patients (29.4%) died. The independent risk factors associated with mortality were multiple infected organs or sites (odds ratio=22.034, 95% confidence intervals=4.348-111.653, P=0.001) and septic shock (odds ratio=27.090, 95% confidence intervals=1.841-398

We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the …

Risk assessment: carbapenemase-producing (OXA-48) Klebsiella pneumoniae ST392 in travellers previously hospitalised in Gran Canaria

The molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae was investigated in the neonatal intensive care unit (NICU) of a university hospital in Italy from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. Molecular typing by pulsed- field gel electrophoresis (PFGE) and dendrogram analysis of ESBL-producing K. pneumoniae isolates identified two distinct PFGE patterns B and C, that were sequentially isolated and that differed from one epidemic clone of PFGE type A isolated during 1996 in the same ward. Antimicrobial susceptibility patterns of ESBL-producing K. pneumoniae epidemic clones of PFGE type B and C showed an identical antibiotype, that differed from clone of PFGE type A for gentamicin resistance. DNA sequencing of amplified blaTEM and blaSHV genes resulted in the detection of a novel blaTEM ESBL gene, blaTEM-136, along with blaSHV-1 gene, in chromosomal and plasmid DNA from K. pneumoniae of ...

Klebsiella pneumoniae is a Gram-negative enterobacterium that has historically been and currently remains, a significant cause of human disease and several kinds of infections in animals. In the present work, trials for the isolation of Klebsiella pneumoniae from diseased and apparently healthy farm animals (cows, sheep, goats and camels) were done for recognition of Klebsiella pneumoniae subspecies. It was noticed that there was a marked variation between incidences of Klebsiella pneumoniae subspecies in examined animals as regards to health condition. The frequency was greater among samples collected from diseased animals 25.2% as compared with apparently healthy one 5.5%. It was found that there was great difference between the prevalence of Klebsiella isolated from various animal origins. On biochemical identification Klebsiella pneumoniae subsp. pneumoniae was the most prevalent followed by Klebsiella pneumoniae subsp. ozaenae and Klebsiella pneumoniae subsp. Rhinoscleromatis. Klebsiella pneumoniae

Objectives: To study the signs and symptoms of neonatal sepsis caused by Klebsiella pneumoniae and the antibiotic sensitivity pattern of Klebsiella pneumoniae in a level 2 neonatal unit in India. Method: This descriptive study was conducted with neonates who were admitted in Sick Newborn Care Unit (level 2 neonatal care unit) and whose blood culture showed growth of Klebsiella pneumoniae in a study period of 1 year. The data was entered into a register and presented by descriptive statistics. Results: Forty eight neonates were included in the study. Predominant presenting symptoms were abdominal distension (75%), petechiae, purpura (62.5%) and sclerema (50%). Klebsiella pneumoniae was highly sensitive to tigecycline and meropenem. Conclusions: Predominant presenting symptoms of neonatal sepsis caused by Klebsiella pneumoniae were abdominal distension, petechiae, purpura and early sclerema. Klebsiella pneumoniae was highly sensitive to tigecycline and meropenem.. Sri Lanka Journal of Child ...

Abstract BACKGROUND AND OBJECTIVE: The spread of carbapenem-resistant Klebsiella pneumoniae especially blaNDM-1-carrying isolates is a great concern worldwide. In this study we describe the molecular basis of carbapenem-resistant K. pneumoniae in three teaching hospitals at Bandar Abbas, south of Iran. MATERIALS AND METHODS: A total of 170 nonduplicate clinical isolates of K. pneumoniae were investigated. Antimicrobial susceptibility test was performed by disc diffusion method. PCR was carried out for detection of carbapenemase (blaKPC, blaIMP, blaVIM, blaNDM, blaSPM, blaOXA-48, and blaOXA-181) and extended-spectrum β-lactamase (blaCTX-M, blaSHV, blaTEM, blaVEB, blaGES, and blaPER). Clonal relatedness of blaNDM-1-positive isolates was evaluated by multilocus sequence typing (MLST). RESULTS: Tigecycline was the most effective antimicrobial agent with 96.5% susceptibility. In addition, 6.5% of the isolates were carbapenem resistant. BlaNDM-1 was identified in four isolates (isolate A-D) and all ...

Domain architectures containing the following SCOP superfamilies 51905,_gap_,55124,56014,_gap_ in Klebsiella pneumoniae subsp. pneumoniae MGH 78578. Domain architectures illustrate each occurrence of 51905,_gap_,55124,56014,_gap_.

TY - JOUR. T1 - Invasive liver abscess syndrome caused by Klebsiella pneumoniae. AU - Yu, Wen-Liang. AU - Chuang, Yin Ching. PY - 2007. Y1 - 2007. N2 - INTRODUCTION - Klebsiella pneumoniae can produce infection at a variety of sites, with the risk being increased in patients with impaired host defenses (eg, diabetes mellitus, alcoholism, malignancy, chronic obstructive pulmonary disease, and glucocorticoid therapy). K. pneumoniae is also associated with a community-acquired primary invasive liver abscess syndrome. In addition to liver abscess, some patients develop metastatic infection at other sites.. AB - INTRODUCTION - Klebsiella pneumoniae can produce infection at a variety of sites, with the risk being increased in patients with impaired host defenses (eg, diabetes mellitus, alcoholism, malignancy, chronic obstructive pulmonary disease, and glucocorticoid therapy). K. pneumoniae is also associated with a community-acquired primary invasive liver abscess syndrome. In addition to liver ...

Klebsiella pneumoniae subsp. pneumoniae ATCC ® 700721D-5™ Designation: Genomic DNA from Klebsiella pneumoniae subsp. pneumoniae Strain MGH78578 TypeStrain=False Application:

This study compared the effect of monotherapy of colistin, tigecycline, and their combination in sepsis model of mice. OXA-48 producing Carbapenem-resistant Klebsiella pneumoniae (CRKP) strain was used in Balb/c mice. The mice were divided into competent and Methylprednisolone acetate (MPA)-treated groups. Each group was sub-divided into (1) colistin or (2) tigecycline monotherapy and (3) colistin/tigecycline combination therapy. After 3 hours of intraperitoneal bacterial inoculation, antimicrobials were administered, and mice were sacrificed at 24 and 48 hours Time-kill curve study demonstrated that colistin sulphate had early bactericidal activity following re-growth. In competent and MPA-treated groups of mice at 24 hours, bacterial counts in liver samples significantly lowered compared to control, however, there were no statistically differences between monotherapy and combination therapy subgroup. Bacterial count in lung samples of competent group was significantly lesser than control for ...

TY - JOUR. T1 - Bloodstream infections by extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in children. T2 - Epidemiology and clinical outcome. AU - Kim, Yun Kyung. AU - Pai, Hyunjoo. AU - Lee, Hoan Jong. AU - Park, Su Eun. AU - Choi, Eun Hwa. AU - Kim, Jungmin. AU - Kim, Je Hak. AU - Kim, Eui Chong. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2002. Y1 - 2002. N2 - To determine the epidemiologic features and clinical outcomes of bloodstream infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolates, cases of bacteremia caused by these organisms in children were analyzed retrospectively. Among the 157 blood isolates recovered from 1993 to 1998 at the Seoul National University Childrens Hospital, the prevalence of ESBL production was 17.9% among the E. coli isolates and 52.9% among the K. pneumoniae isolates. The commonest ESBLs were SHV-2a and TEM-52. A novel ESBL, ...

Doxycycline 100 mg taken twice daily for 7 days. Can nitrofurantoin mono/mac be used to treat Strep. Urinary Tract Infection caused by Klebsiella.Thrush effects sunlight doxycycline mono is good for chlamydia lymes disease dosage for klebsiella uti. Does hyclate treat acne. doxycycline hyclate treat an.Definitions of Q fever, synonyms,. Q fever in pregnancy is especially difficult to treat because doxycycline and ciprofloxacin are. Klebsiella pneumoniae.. Some of them primarily cause respiratory problems (Hemophilus influenzae, Klebsiella pneumoniae,. Can doxycycline treat bacterial infections of the eye?.Publications de Recherche 1. AUTHIÉ E.,. BOUSQUET-MELOU A. Emergence of resistant Klebsiella pneumoniae in the. dietary doxycycline supplied with and.Walgreens Viagra Price - Online Pharmacy::Buy Online No Prescription Needed. Cheap price. Overnight Delivery. Discounts up to 80%. Free shipping available ...

The accurate identification of a pathogen beyond the species level is critical in epidemiological studies and investigations of nosocomial outbreaks of infection. The clonal relatedness of 66 multidrug resistant (MDR) strains of extended spectrum beta-lactamase (ESBL) producing K. pneumoniae isolated from clinical specimens from hospitalized patients at a Jamaican hospital during a 5 year period were determined by pulsed field gel electrophoresis (PFGE). A total 10 different ESBL producing K. pneumoniae genotypes designated Clones I-X were found. The most frequently occurring strains belonged to Clones I (21/66, 32%), II (15/66, 26%), III (13/66, 20%) and IV (8/66, 12%) which accounted for 86% (57/66) of ESBL producing K. pneumoniae strains over the 5 year period. The remaining 9 (14%) cases of ESBL producing K. pneumoniae were due to strains of Clones V-X. The 4 predominant clones persisted for several years in the hospital. The clonal and temporal distribution of the MDR ESBL producing K. pneumoniae

OBJECTIVES: Infections caused by multidrug-resistant Enterobacterales pose a significant challenge to clinical patient care, particularly in resource-constrained settings where epidemiological data on antimicrobial resistance are scarce. The aim of this study was to determine the prevalence of extended spectrum beta-lactamase-(ESBL)-producing Klebsiella pneumoniae among clinical samples from a teaching hospital in Bouake, central Cote dIvoire. METHODS: Clinical specimens were collected from sterile and non-sterile body sites and were subjected to microbiological diagnostics (April 2016-June 2017). The antimicrobial susceptibility patterns of K. pneumoniae were analysed using automated resistance testing and double-disk diffusion to test for ESBL production. Multiplex PCR was carried out to determine the presence of the resistance-conferring genes blaCTX-M, blaSHV and blaTEM. RESULTS: A total of 107 isolates were included, most of which were obtained from bloodstream (39%; n = 42) and urinary ...

Thank you for sharing this Journal of Clinical Microbiology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...

Carbapenem-hydrolyzing β-lactamases can be metallo-β-lactamases (7), expanded-spectrum oxacillinases (8), or Ambler class A enzymes (1, 2, 4, 6, 9-12). The class A KPC β-lactamases hydrolyze all β-lactams except for cephamycins and have been rarely reported in studies of enterobacterial species (Klebsiella pneumoniae, Klebsiella oxytoca, Salmonella enterica, Enterobacter sp.) found in New England and the mid-Atlantic region of the United States; when they are reported, they are found to come from New York City hospitals in particular (2, 4, 6, 9-12). We report here a K. pneumoniae isolate from Paris that produced β-lactamase KPC-2.. An 80-year-old man with a 5-year history of prostatic carcinoma and metastasis was admitted in February 2005 at the Cochin hospital in Paris for acute urine retention in the right nephrostomy. The bilateral nephrostomy was performed in December 2004 at a New York City hospital. No hospitalization or antibiotic treatment is known to have taken place during the ...

Klebsiella pneumoniae is the major cause of community-acquired pyogenic infections in Taiwan. This retrospective study evaluated the clinical and microbiological characteristics of bacteremic community-acquired pneumonia due to K. pneumoniae in Taiwanese adults. The clinical characteristics of bacteremic community-acquired pneumonia (CAP) in adults due to K. pneumoniae were compared to those of adults with bacteremic CAP due to Streptococcus pneumoniae at a tertiary medical center in Taiwan from 2001-2008. Risk factors for mortality of bacteremic CAP due to K. pneumoniae were analyzed. All clinical isolates of K. pneumoniae were examined for capsular serotypes, hypermucoviscosity phenotype, aerobactin and rmpA gene. K. pneumoniae was the dominant cause of bacteremic CAP and was associated with a more fulminant course and a worse prognosis than bacteremic CAP due to Streptococcus pneumoniae. Initial presentation with septic shock and respiratory failure were independent risk factors for both early and

Klebsiella pneumoniae is a leading cause of hospital-acquired urinary tract infections and pneumonia worldwide, and is responsible for many cases of pyogenic liver abscess among diabetic patients in Asia. A defining characteristic of this pathogen is the presence of a thick, exterior capsule that has been reported to play a role in biofilm formation and to protect the organism from threats such antibiotics and host immune challenge. We constructed two knockout mutants of K. pneumoniae to investigate how perturbations to capsule biosynthesis alter the cellular phenotype. In the first mutant, we deleted the entire gene cluster responsible for biosynthesis of the extracellular polysaccharide capsule. In the second mutant, we deleted the capsule export subsystem within this cluster. We find that both knockout mutants have lower amounts of capsule but produce greater amounts of biofilm. Moreover, one of the two mutants abolishes fimbriae expression as well. These results are expected to provide insight into

Lettice. am May 25th, I TOOK 4 HYDRODOON AETAMINOPHIN IN A 2hr. gargle [HOST]se I DONT THINK How much hydrocodone is too · Much hydrocodone with alcohol. If youre a new with a hydrocodone prescription and you find youre taking to take large klebsiella pneumoniae resistances to ampicillin for pain relief, row to your doctor about adjusting your lifestyle or trying a different medication, so that you can cause the risk of aspirin overdose on hydrocodone or any of the other medications in your. If youre taking 5 mg klebsiella pneumoniae resistances to ampicillin with no more than mg acetaminophen, its pretty to take 2 tablets every six months. You can give an end to your hydrocodone (Norco) decennium and addiction problem, and save more about what happens when you have help in this Norco Bloodstream Treatment Program and Help. Too much hydrocodone will help down his respiratory system But Im thin sure hed be lower from the tylenol way before hydrocodone became an infection Hes not going to ...

TY - JOUR. T1 - Synergistic activity of 5-trifluoromethylthioribose and inhibitors of methionine synthesis against Klebsiella pneumoniae. AU - Tower, P. A.. AU - Johnson, L. L.. AU - Ferro, A. J.. AU - Fitchen, J. H.. AU - Riscoe, M. K.. PY - 1991. Y1 - 1991. N2 - 5-Methylthioribose (MTR) is an intermediate in the methionine recycling pathway of organisms containing the enzyme MTR kinase. Analogs of MTR have been proposed as a new class of antimicrobial agents because of their ability to perturb the growth of MTR kinase-containing pathogens through inhibition of methionine salvage or by conversion to toxic products. One such analog, 5- trifluoromethylthioribose (TFMTR), has demonstrated potent inhibitory effects on the growth of Klebsiella pneumoniae (A. G. Gianotti, P. A. Tower, J. H. Sheley, P. A. Conte, C. Spiro, J. H. Fitchen, and M. K. Riscoe, J. Biol. Chem. 265:831-837, 1990). Although the mode of action of TFMTR has yet to be determined, it is believed that the drug is converted to the ...

Complete genome sequence of Klebsiella pneumoniae subsp. pneumoniae HS11286, a multidrug-resistant strain isolated from human sputum ...

This paper shows that magA is an important virulence gene in invasive K. pneumoniae strains causing primary liver abscess and septic metastatic complications. Disruption of magA in NTUH-K2044 results in complete loss of resistance to serum and phagocytosis, along with a ,105-fold increase in LD50 to mice. The virulence was significantly but not completely restored by complementation using a magA-containing plasmid, suggested that adjacent loci might also be contributing to virulence. Although other genetic loci, including the cps cluster (37), wb cluster (38), and rmpA (39, 40) might also be involved in bacterial virulence, only magA displayed a significantly higher prevalence rate in invasive strains (98 vs. 29%; P , 0.0001). Thus, magA meets the criteria of molecular Kochs postulates (45) for a virulence gene associated with this type of invasive K. pneumoniae disease. Thus, this locus can be used as a marker for rapid molecular diagnosis.. In the present work, our data provided supports for ...

Background: We describe and evaluate our outbreak of carbapenem-resistant K. pneumoniae transmitted by contaminated duodenoscopes during endoscopic retrograde cholangiopancreatography (ERCP) procedures. Methods: An outbreak investigation was performed when Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) were identified from bile specimens of 4 patients. The investigation included medical record review, practice audits, and surveillance cultures of duodenoscopes and environmental sites. If available, clinical specimens were obtained from patients who had undergone ERCP in the previous 3 months. Carbapenem-resistant Enterobacteriaceae (CRE) screening cultures were performed to identify additional patients until no CRE cases were detected during 2 consecutive weeks. Pulsed-field gel electrophoresis (PFGE) of KPC-KP isolates was implemented. Results: In total, 12 cases were identified with exposure to duodenoscope from February 2019 through April 2019, including 6 cases with ...

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...

Klebsiella pneumoniae is a recognised agent of multidrug-resistant (MDR) healthcare-associated infections; however, individual strains vary in their virulence potential due to the presence of mobile accessory genes. In particular, gene clusters encoding the biosynthesis of siderophores aerobactin (iuc) and salmochelin (iro) are associated with invasive disease and are common amongst hypervirulent K. pneumoniae clones that cause severe community-associated infections such as liver abscess and pneumonia. Concerningly, iuc has also been reported in MDR strains in the hospital setting, where it was associated with increased mortality, highlighting the need to understand, detect and track the mobility of these virulence loci in the K. pneumoniae population. Here, we examined the genetic diversity, distribution and mobilisation of iuc and iro loci amongst 2503 K. pneumoniae genomes using comparative genomics approaches and developed tools for tracking them via genomic surveillance. Iro and iuc were detected

The enzyme propanediol oxidoreductase, which converts the lactaldehyde formed in the metabolism of fucose and rhamnose into propane-1,2-diol under anaerobic conditions, was investigated in Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium. Structural analysis indicated that the enzymes of E. coli and K. pneumoniae have the same Mr and pI, whereas that of Salm. typhimurium also has the same Mr but a slightly different pI. One-dimensional peptide mapping showed identity between the E. coli and K. pneumoniae enzymes when digested with α-chymotrypsin, Staphylococcus aureus V8 proteinase or subtilisin. In the case of Salm. typhimurium, this held only for the subtilisin-digested enzymes, indicating that the hydrophobic regions were preserved to a considerable extent. Anaerobically, the three species induced an active propanediol oxidoreductase when grown on fucose or rhamnose. An inactive propanediol oxidoreductase was induced in Salm. typhimurium by either fucose or rhamnose under ...

Colistin and polymyxin B MICs were determined for 106 carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates using Sensititre Research Use Only GNX2F plates (Thermo Fisher) and compared to CLSI broth macrodilution (BMD) as the reference method. For colistin, EUCAST breakpoints were applied and …

So Im sure some of you have read the battles Ive had with MRSA osteomyelitis and my recent hemipelvectomy, but there is something else I have been battling this year, an ESBL Klebsiella pneumoniae UTI. I hadnt had a UTI in over 4 years, so I can guarantee I picked this up nosocomially since Ive been in the hospital about 9 months this year. Ive been on IV antibiotics for 11 months, but each time I would come off of them, I would go septic (either due to the osteo or the UTI) within 2

Ghina Ali, Christophe Rihouey, Didier Le Cerf, Luc Picton. Effect of carboxymethyl groups on degradation of modified pullulan by pullulanase from Klebsiella pneumoniae. Carbohydrate Polymers, Elsevier, 2013, 93 (1), pp.109 - 115. ⟨10.1016/j.carbpol.2012.07.039⟩. ⟨hal-01866742⟩ ...

BioAssay record AID 93884 submitted by ChEMBL: Minimum inhibitory concentration of compound for its inhibitory activity against Klebsiella pneumoniae growth at 2.4 ug/mL.

Similarly increased serum IgA1 and IgA2 subclass antibody levels against Klebsiella pneumoniae bacteria in ankylosing spondylitis patients with/without extra-articular ...

1OZH: The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.

Stonyfield is extending its voluntary recall of YoBaby Peach/Pear Yogurt first issued on April 25, 2014. The recall now includes an addition 1,344 six-packs shipped to 271 more stores. The stores that carry the product are mostly Safeway and Fred Meyer stores in Oregon, Washington, Montana, Idaho, Alaska, and northern California. The yogurts may contain Klebsiella pneumoniae, a bacteria that usually dont cause illness when they are in food, but they sometimes do. The bacteria may have variations in their genes, which could change how they cause illness, along with the severity of that illness. The risk of illness also depends on the health and genetic make-up of the person who eats them. Anyone who does become sick after eating this yogurt can suffer watery diarrhea, nausea, vomiting, … [Read more...] ...

The aloe cactus, actually a desert succulent, has been touted as a miracle plant, and its antibiotic activity is definitely a part of its reputation. The gel in the leaves of the aloe plant contains a fairly long list of unique substances that account for many of its healing properties.. Functions:. Fighting viruses is not aloes only antibiotic activity, however. It is also an antibacterial and antifungal. Aloe is a known bactericide against a dozen or so different kinds of bacteria, including the pneumonia-causing bacterium Klebsiella pneumoniae. It has also been shown to inhibit the fungus Candida albicans, responsible for most yeast infections.. The common oral preparation of the aloe plant currently available is aloe vera juice, a partially refined and diluted extract of the active gel. It is important to select a high-quality product that is as much like the inner gel of the plant as possible and that has not been subjected to high heat or unnecessary filtering during its manufacture. ...

Infection with colistin-resistant organisms is associated with a substantially increased risk for mortality, and options for treatment are limited (9-11, 17-20). Prior studies have typically been relatively small or limited in scope, either following a relatively small number of patients (9, 10, 17, 18, 24, 26, 30-33) or focusing on a single organism (16, 21, 22, 27), and none have resulted in a clinically meaningful prediction tool. Our score can be calculated by providers at the time of decision making without the help of a computer. This potentially more accurately reflects a patients risk for Colr organisms than a hospital-wide or unit-specific antibiogram, which can provide only a flat percent expected susceptibility for a given organism and is not useful for the management of rare events. Additionally, most hospital labs do not routinely test for colistin susceptibility, and it is rarely in a hospitals antibiogram. All information used in the models was from standardized data fields that ...

المجلة العراقية للعلوم Iraqi Journal of Science A Refereed Science Journal Issued by the College of Science University of Baghdad Baghdad-Iraq. مجلة علمية محكمة تصدرها كلية العلوم- جامعة بغداد، بغداد- جمهورية العراق EISSN: 2312-1637 ISSN: 0067-2904

AbstractObjective: To elucidate whether the genetic platforms of blaCTX-M contribute to the phenotypes of multi-drug-resistance in the first carbapenemase-producing K. pneumoniae strains isolated in Chile. Method: Twenty-two carbapenemase-producing K. pneumoniae strains isolated from different Chilean patients and hospitals were studied. Their genetic relatedness was assessed by PFGE and MLST. The levels of antibiotic resistance were evaluated by determining the minimum inhibitory concentration of various antimicrobials. In addition, several antibiotic resistance genes of clinical relevance in Chile were investigated. The prevalence, allelic variants and genetic platforms of blaCTX-M were determined by PCR and sequencing.Results: Out of the twenty two strains studied, twenty carry KPC, one carries NDM-1 and one carries OXA-370. The PFGE analysis showed three clades with a genetic relatedness |85%, two formed by four strains and one by eight strains. The other strains are not genetically related, and a

Epidemiological study of multidrug resistant Klebsiella pneumoniae by pulsed field gel electrophoresis at the University Hospital of the West Indies 2000-2004. Caribbean Health Research Council 53rd Annual Council and Scientific Meetings, April 2008, Paramaribo, Suriname ...

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Barreto, H. A.. Cunha, F. A. Cunha, M. da C. dos S. O.. Menezes, E. A.. Resumo. A produção de betalactamases de espectro ampliado (Extended-Spectrum Betalactamase - ESBL) vem sendo detectada mundialmente, e em número crescente, especialmente em Escherichia coli e Klebsiella spp, importantes patógenos nosocomiais. O presente trabalho analisou isolados de pacientes da clínica e internados de um hospital da cidade de Fortaleza de E. coli e Klebsiella spp, no período de janeiro a dezembro de 2008, objetivando: abordar a importância da detecção de ESBL em laboratórios de microbiologia clínica; evidenciar as possíveis falhas terapêuticas de diversos antimicrobianos em pacientes com infecções por bactérias produtoras de ESBL e demonstrar que a sua detecção pode e deve ser uma prática rotineira em laboratórios de microbiologia clínica. O isolamento das bactérias foi realizado por intermédio dos meios de cultura padronizados para cada tipo de amostra. A identificação, assim como ...