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Other antibiotics used to treat susceptible isolates include ampicillin/sulbactam, piperacillin/tazobactam, ticarcillin/clavulanate, ceftazidime, cefepime, levofloxacin, norfloxacin, moxifloxacin, mer... more
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The rise of superbugs. Earlier this year, the World Health Organization (WHO) published a report on anti-microbial resistance, with a special emphasis on antibiotic resistance of so-called "superbugs". Such bacteria pose the greatest threat to human health due to their resistance to several different antibiotics. Among these superbugs is Klebsiella, which can cause severe and often fatal infections of the bloodstream and lungs. Klebsiella has been reported to be resistant to common classes of antibiotics and to a great extent also to carbapenems, the last resort to treat severe nosocomial infections.. Treatment options beyond common antibiotics. The researchers around Pavel Kovarik at MFPL and Jose Bengoechea at Queens University Belfast now discovered how immune cells arriving at the site of infection communicate and join forces to eradicate Klebsiella during lung infections. Their study suggests that future therapies of severe Klebsiella infections could target the immune system, rather than ...
A team of US researchers has discovered that three different species of Klebsiella bacteria can cause life-threatening infections in hospital patients and that all three share genes that confer resistance to the most commonly used antibiotics. The study, published this week in mSphere®, an open-access journal of the American Society for Microbiology, improves physicians understanding of Klebsiella infections and could point toward better ways to fight multi-drug resistant strains of these bacteria.
Bacteria of the genus Klebsiella are widely distributed in nature, in the soil and in water. They are also part of the normal flora of the intestinal tract, but usually in low numbers compared with E. coli. Klebsiella, especially strains of the species K. pneumonia, are opportunistic pathogens that can cause pneumonia, urinary tract infections, and bacteremia. In recent years there has been an increase in Klebsiella infections, especially in hospitals and due to multiple-antibiotic resistant strains. The most striking difference between most strains of Klebsiella and its close relatives E. coli and Salmonella is that Klebsiella cells have a thick coat of slime or extracellular polysaccharide which is called a "capsule". The capsule protects the cells from dessication, and may also protect them from phagocytosis when they are in an animal host. Surprisingly, many strains of Klebsiella can fix nitrogen, i.e., they can reduce atmospheric nitrogen to ammonia and amino acids.Strain 342 was isolated ...
Question - Skin infection on face, klebsiella, bactrim . Ask a Doctor about diagnosis, treatment and medication for Klebsiella infection, Ask an Internal Medicine Specialist
Hi I hope you folks dont mind, I am posting this for my mama. She has fibro and last year contracted a life threatening Klebsiella infection which...
The pipeline guide provides a snapshot of the global therapeutic landscape of Klebsiella Pneumoniae Infections. The pipeline guide reviews pipeline therapeutics for Klebsiella Pneumoniae Infections by companies and universities/research institutes based on information derived from company and industry-specific sources.
TY - JOUR. T1 - Prevention of colonization and infection by klebsiella pneumoniae carbapenemase-producing enterobacteriaceae in long-term acute-care hospitals. AU - Hayden, Mary K.. AU - Lin, Michael Y.. AU - Lolans, Karen. AU - Weiner, Shayna. AU - Blom, Donald. AU - Moore, Nicholas M.. AU - Fogg, Louis. AU - Henry, David. AU - Lyles, Rosie. AU - Thurlow, Caroline. AU - Sikka, Monica. AU - Hines, David. AU - Weinstein, Robert A.. PY - 2015/4/15. Y1 - 2015/4/15. N2 - Background. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (hereafter "KPC") are an increasing threat to healthcare institutions. Long-term acute-care hospitals (LTACHs) have especially high prevalence of KPC. Methods. Using a stepped-wedge design, we tested whether a bundled intervention (screening patients for KPC rectal colonization upon admission and every other week; contact isolation and geographic separation of KPC-positive patients in ward cohorts or single rooms; bathing all patients daily with ...
Klebsiella Pneumoniae Infections - Market Insights, Epidemiology and Market Forecast - 2025 is a market research report available at US $5750 for a Single User PDF License from RnR Market Research Reports Library.
TY - JOUR. T1 - Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. AU - Kim, Mi Na. AU - Yong, Dongeun. AU - An, Dongheui. AU - Chung, Hae Sun. AU - Woo, Jun Hee. AU - Lee, Kyungwon. AU - Chong, Yunsop. PY - 2012/4/1. Y1 - 2012/4/1. N2 - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four patients with no history of traveling abroad who stayed for 21 to 205 days in a tertiary care hospital. All were sequence type (ST) 340 and had nearly identical XbaI pulsed-field gel electrophoresis (PFGE) patterns. The bla NDM-1-carrying plasmids were in the IncN group, with sizes ranging from 50 to 200 kb. These findings suggest that NDMKP had already been introduced into South Korea before this clustering was found.. AB - In November 2010, NDM-1-producing Klebsiella pneumoniae (NDMKP) was identified for the first time in South Korea from four ...
Klebsiella pneumonia (KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. It is typically due to aspiration and alcoholism may be a risk factor, though it is also commonly implicated in hospital-acquired urinary tract infections, and COPD(chronic obstructive pulmonary disease) individuals Individuals with Klebsiella pneumonia tend to cough up a characteristic sputum, as well as having fever, nausea, tachycardia and vomiting. Klebsiella pneumonia tends to affect people with underlying conditions, such as alcoholism. The cause of the condition Klebsiella pneumonia is Klebsiella pneumoniae which is gram-negative, as well as rod-shaped, glucose-fermenting, facultative anaerobic bacterium. In terms of the pathophysiology of Klebsiella pneumonia we see neutrophil myeloperoxidase defense against K P.Oxidative inactivation of elastase is involved, while LBP helps transfer bacteria cell wall elements to the cells. Klebsiella resistant strains have been recorded in USA with a ...
It appears as a mucoid lactose fermenter on MacConkey agar. Klebsiella pneumonia ( KP) is a form of bacterial pneumonia associated with Klebsiella pneumoniae. B) A total of 5, 475 samples collected from children resulted in laboratory- positive cultures; the 5 most frequently occurring bacterial species accounted for [ approximately equal to] 70% of total bacterial infections, and Klebsiella pneumoniae ( white bar) was the third most dominant ( 710 isolates). Klebsiella pneumoniae is a bacterium that causes UTIs. Pneumoniae is the most prevalent and clinically important. For example, Klebsiella must enter the respiratory ( breathing) tract to cause pneumoniae, or the blood to cause a bloodstream infection. Klebsiella pneumoniae și articulaţii. To get a Klebsiella infection, a person must be exposed to the bacteria. The organism resides in the upper respiratory tract and gastrointestinal tract of healthy individuals. Klebsiella pneumoniae is a bacterial organism that is responsible for causing ...
The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and the human host. Sequence analysis revealed novel plasmid-located virulence factors, including a cluster for glycogen synthesis. Glycogen production is considered to be one of the possible adaptive responses to long-term survival and growth in environments outside the host. Chromosomally-encoded virulence traits in the clone
Abstract. We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14.. Keywords: Klebsiella pneumoniae Carbapenemase (KPC), multidrug resistance, whole genome sequencing ...
To the Editor: Until a few years ago, the most frequent microbiologically documented cause of severe bloodstream infections among patients with hematologic malignancies was gram-positive bacteria (1). However, over the years, gram-negative bacteria have become the main infectious cause of death among patients with hematologic malignancies, and rates of different phenotypes associated with antimicrobial drug resistance are increasing (2). This trend could be the result of increasing empirical use of antimicrobial drug therapy and prophylaxis and use of new, more effective antimicrobial drugs. In particular, over the past few years at our hospital (Agostino Gemelli Teaching Hospital, Rome, Italy), we have observed a progressive increase in bloodstream infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp), which are responsible for a dramatic new scenario.. We reviewed records of all patients affected by hematologic malignancies who were admitted to the hospital ...
The global emergence of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) multilocus sequence type ST258 is widely recognized. Less is known about the molecular and epidemiological details of non-ST258 K. pneumoniae in the setting of an outbreak mediated by an endemic plasmid. We describe the interplay of blaKPC plasmids and K. pneumoniae strains and their relationship to the location of acquisition in a U.S. health care institution. Whole-genome sequencing (WGS) analysis was applied to KPC-Kp clinical isolates collected from a single institution over 5 years following the introduction of blaKPC in August 2007, as well as two plasmid transformants. KPC-Kp from 37 patients yielded 16 distinct sequence types (STs). Two novel conjugative blaKPC plasmids (pKPC_UVA01 and pKPC_UVA02), carried by the hospital index case, accounted for the presence of blaKPC in 21/37 (57%) subsequent cases. Thirteen (35%) isolates represented an emergent lineage, ST941, which contained pKPC_UVA01 in 5/13 (38%
TY - CONF. T1 - Successful treatment of KPC-3 Klebsiella Pneumoniae ST258 clone with a combination of high-dose tigecycline and colistin in ICU: a case series report.. AU - Gulotta, Gaspare. AU - Raineri, Santi Maurizio. AU - Bonura, Celestino. AU - Pantuso, Gianni. AU - Cortegiani, Andrea. AU - Giarratano, Antonino. AU - Agrusa, Antonino. AU - Di Carlo, Paola. AU - Cocorullo, Gianfranco. AU - Giammanco, Anna. AU - Mammina, Caterina. PY - 2011. Y1 - 2011. N2 - INFECTIONS CAUSED BY KLEBSIELLA PNEUMONIAE SEQUENCE TYPE 258 PRODUCING K. PNEUMONIAE CARBAPENEMASE 3 (KPC-Kp)HAVE WIDELY EMERGED AND BOTH INDIVIDUAL CASES AND OUTBREAKS OF COLONIZATION OR INFECTION HAVE BEEN REPORTED IN PALERMO, ITALY.OBIETTIVO: THIS IS A RETROSPECTIVE CASE SERIES THAT DESCRIBES THE CLINICAL AND MICROBIOLOGIC OUTCOMES OF 16 PATIENTS WHO RECEIVED A COMBINATION OF HIGH-DOSE TIGECYCLINE AND COLISTIN FOR TREATMENT OF VAP (4 CASES) AND SEVERE BACTERAEMIA (12 CASES) DURING THE YEARS 2009-2011. 11 OUT OF THE 16 CASES WERE POST ...
Carbapenemase-producing Enterobacteriaceae (CPE) have emerged in recent years as being among of the most important threats to public health. CPE have been detected in almost all European countries, although with a highly variable geographical distribution (1). Some high-risk clones of Klebsiella pneumoniae, mainly KPC-producing sequence type 258 (ST258) but also OXA-48-producing ST395 or ST15, have been implicated in the worldwide spread of carbapenemases (2-4).. Previous studies have shown that the population of Escherichia coli strains resistant to amoxicillin-clavulanic acid is less diverse than the susceptible population (5). Little is known about the population structure of carbapenemase-producing K. pneumoniae in comparison with the carbapenem-susceptible population. The aim of this study was to test the hypothesis that the carbapenemase-producing K. pneumoniae strains isolated in Spanish hospitals constitute a distinct and more homogeneous population than the carbapenemase-susceptible ...
Klebsiella pneumoniae is gram negative rod which present with large mucoid capsulre. The common virulence factors of klebsiella pneumonia may include protease, cell wall endotoxin and mucoid capsule.
klebsiella. FAQ. Medical Information. Klebsiella pneumoniae; Klebsiella oxytoca; Enterobacteriaceae; Enterobacter;. Ciprofloxacin; HLA-B27 Antigen; Aminoglycosides.. Characterization of an IncN2-type bla NDM-1-carrying plasmid in Escherichia coli ST131 and Klebsiella pneumoniae ST11 and ST15 isolates in Thailand.ColonizaciÓn fecal por cepas de Klebsiella pneumoniae productoras de betalactamasas de espectro. and more than 90% were also susceptible to ciprofloxacin and.Community-acquired Klebsiella pneumoniae meningitis in an alcoholic patient with an infected pancreatic pseudocyst; a case report and review of literature.%T Urinary pharmacodynamics of low-dose ciprofloxacin and ofloxacin %A STEIN G. E. %A SCHOOLEY S. MIC = 4; Klebsiella pneumoniae, MIC = 4; Staphylococcus.Titre du document / Document title Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type β-lactamase-producing.Titre du document / Document title Extended-spectrum beta-lactamases ...
Background and Objective: The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae. The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic methods. Method: Out of 800 strains, 270 K. pneumoniae strains (33.7%), were obtained. Antibiotic susceptibility test was performed by disk diffusion method in accordance with CLSI guidelines. Carbapenem resistant strains were identified by the Modified Hodge Test based on CLSI instruction and PCR for surveying the presence of bla-KPC gene. Results: A total 270 K. pneumoniae strains were collected. Antibiotic susceptibility test results showed the highest and lowest resistance was related to piperacillin (60.6%) and carbapenems (14.6%) respectively. 80.5% (33 of 41) isolates were positive by MHT, but all of them (100%) were negative for amplification of the bla-KPC
Discussion. Incidence and trends of K. pneumoniae HAI. Yogaraj et al.[5] found that in their experience, in Washington DC, bloodstream infections accounted for 28% of HAI in the PICU, followed by ventilator-associated pneumonias, which accounted for a further 21%. Lakshmi et al.[6] reported on 116 episodes of bloodstream infection in 86 patients in India in 2006, with an incidence of 31.2 episodes/1 000 patient days. Marra et al.[7] found that K. pneumoniae ranked among the top ten pathogens causing bloodstream infection in the USA and Canada, and that it was the third most prevalent pathogen isolated in Latin America.. K. pneumoniae HAI is a significant problem in the delivery of intensive care services, and prolongs hospital stay. In this study, there was an incidence for K. pneumoniae HAI of 10/1 000 admissions, with a range of 0 - 28.8/1 000 patients per annum once the PICU was fully commissioned. Unfortunately, as this was a retrospective study, we were unable to access data on patient ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Les bêta -lactamines. ampicilline (po), amoxicilline. important de connaître les antécédents dallergie à une bêta lactamine et de tenir compte des.La première étape de la réaction enzymatique variera en fonction du substrat (affinité) tels pénicilline G, oxacilline, ampicilline. beta-lactamase TLA-2). 6.beta-lactamase-producing Klebsiella pneumoniae infection in. generation cephalosporins, ceftazidime, ampicillin, aminoglycosides and vancomycin/teicoplanin were.Original Article Antimicrobial. examined for production of beta-lactamase using a. Ampicillin (E) 26.7 1.3 72 0.5 16 ≤1 ≥4, in ...
Klebsiella pneumoniae ATCC ® BAA-1705D-5™ Designation: Genomic DNA from Klebsiella pneumoniae strain ART 2008133 TypeStrain=False Application: Source culture is Modified Hodge Test (MHT) positive control designation Source culture produces Klebsiella pneumoniae carbapenemase (KPC)
TY - JOUR. T1 - Effect of piperacillin-tazobactam vs meropenem on 30-day mortality for patients with e coli or Klebsiella pneumoniae bloodstream infection and ceftriaxone resistance. AU - Harris, Patrick N.A.. AU - Tambyah, Paul A.. AU - Lye, David C.. AU - Mo, Yin. AU - Lee, Tau H.. AU - Yilmaz, Mesut. AU - Alenazi, Thamer H.. AU - Arabi, Yaseen. AU - Falcone, Marco. AU - Bassetti, Matteo. AU - Righi, Elda. AU - Rogers, Benjamin A.. AU - Kanj, Souha. AU - Bhally, Hasan. AU - Iredell, Jon. AU - Mendelson, Marc. AU - Boyles, Tom H.. AU - Looke, David. AU - Miyakis, Spiros. AU - Walls, Genevieve. AU - Al Khamis, Mohammed. AU - Zikri, Ahmed. AU - Crowe, Amy. AU - Ingram, Paul. AU - Daneman, Nick. AU - Griffin, Paul. AU - Athan, Eugene. AU - Lorenc, Penelope. AU - Baker, Peter. AU - Roberts, Leah. AU - Beatson, Scott A.. AU - Peleg, Anton Y.. AU - Harris-Brown, Tiffany. AU - Paterson, David L.. AU - for the MERINO Trial Investigators and the Australasian Society for Infectious Disease Clinical ...
Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence ...
The treatment outcome of 35 cases of bacteremia due to Klebsiella pneumoniae isolates producing TEM-52 extended-spectrum β-lactamase was studied. Twenty-eight cases, classified as "nonfatal disease" using the McCabe and Jackson classification, were investigated with regard to ciprofloxacin and imipenem response. Because ciprofloxacin was active in vitro against 21 of 28 isolates, only the treatment outcome of the ciprofloxacin-susceptible subgroup was evaluated. Eight of 10 cases occurred in patients who experienced a complete response to imipenem; 2 of 10 failed to respond. In contrast, only 2 of 7 cases had a partial response to ciprofloxacin, and, in 5 of 7 cases, the treatment failed. Statistical analysis revealed a significant difference in the treatment outcome of the 2 groups (P = .03). Because the isolates had minimum inhibitory concentrations of ciprofloxacin close to the susceptibility breakpoint, treatment failure could be ascribed to the inability of the drug to reach therapeutic ...
Objective(s): Infections due to carbapenemase-producing Klebsiella pneumoniae are associated with high morbidity and mortality. In this study, we report a hospital outbreak due to co-producing OXA-48 and NDM-1 K. pneumoniae clone. The aim of the study is to investigate the clonal relationship of strains, risk factors of outbreak and infection control measures.Materials and Methods: Once an outbreak was suspected at the end of December 2017 in our intensive care unit (ICU), carbapenem resistance K. pneumoniae identified in patients specimens. An outbreak analysis was begun to determine the risk factors and dissemination of the cases. A case-control study was conducted to determine the risk factors. To control the outbreak; tight contact prevention, good clean-up the medical devices and hospital environment, were done. Staff training programs such as hand hygiene, disinfection, wearing aprons, good cleaning were created. Carbapenem resistance genes determined by PCR. Clonal relationships of strains
Nosocomial infection with Klebsiella pneumoniae has a worldwide distribution. The presence of invasive devices, contamination of respiratory support equipment, immunocompromised status, treatment in an intensive care unit or nursing home, and use of antibiotics are factors that increase the likelihood of nosocomial infection. Acquisition of K. pneumoniae has become a major problem in many hospitals because of resistance to multiple antibiotics, the scarcity of therapeutic options left to treat these patients, and the association of invasive infections with disturbingly high mortality rates (6).. Carbapenem resistance in K. pneumoniae strains can arise by the loss or modification of porins (OmpK35 and OmpK36) associated with the production of expanded-spectrum or AmpC β-lactamases or by the acquisition of resistance genes encoding metallo-β-lactamases (MBLs) and nonmetallo-carbapenemases (KPC, GES, or OXA types) (9, 16, 17, 21).. In the last several years, an extremely drug-resistant K. ...
The increasing speed and decreasing cost of high-throughput DNA sequencing technologies are enabling its application to the practice of medicine (35). Here, we tested whether genome sequencing could help to unravel a nosocomial outbreak and affect hospital infection control decisions. We sequenced patient and environmental isolates within a clinically relevant turnaround time during a hospital KPC-K. pneumoniae outbreak. Among the key insights provided by sequencing were that (i) the outbreak was monoclonal, despite a 3-week interval between the index case and the identification of subsequent cases, (ii) transmission likely occurred from at least two different sites of the index patient, (iii) at least three independent transmission events from the index patient led to two major clusters of colonized patients, (iv) one patient could be linked to a contaminated ventilator, and (v) a small number of putative resistance mutations could be identified in newly colistin-resistant isolates. Moreover, ...
Eighteen K. pneumoniae strains were identified from patients: seven and nine strains from clinical and screening specimens respectively; and two strains from ventilator touchscreen and suction device manometer. M13 fingerprint analysis revealed closely related strains confirmed by MLST (ST15) performed in selected strains. All of the K. pneumoniae isolates had the same pattern of multiresistance.Molecular experiments revealed that CTX-M-15and SHV-28 were the prevalentESBLs. The outbreak was controlled and eliminated by a combination of intensive infection control measures and rigorous local surveillance. These safeguards remain in place and no outbreaks were detected since January 2010. ...
The articles reports on the discovery of a carbapenem-resistant Klebsiella pneumoniae strain producing a Verona integron-encoded metallo-beta-lactamase (VIM) carbapenemase. The strain, not common among Enterobacteriaceae in the U.S., was detected from an American tourist who was vacationing in Greece. Initial diagnosis was Clostridium difficile infection but was proven otherwise when the patient was transferred to a U.S. hospital. No other patients in the hospital was found to have the strain ...
In the last decade, a new hypervirulent (hypermucoviscous) variant of Klebsiella pneumoniae has been described (1). Most isolates of hypervirulent K. pneumoniae are very susceptible to antimicrobials (except ampicillin). However, a multidrug-resistant and hypervirulent variant of K. pneumoniae has also been described as the next "superbug" (2). On the other hand, Klebsiella variicola is a Gram-negative rod of the Enterobacteriaceae family; it was described as a new bacterial species in 2004 (3). Currently, K. variicola is known to be an endophyte of plants (3, 4), a symbiont in insects (5), and a pathogen in humans (3). A susceptible and multiresistant phenotype of K. variicola has been identified, corresponding to an extended spectrum β-lactamase (ESBL)-producing K. variicola, encoding the SHV-type and CTX-M-15 genes (6, 7).. It is difficult to distinguish K. variicola from K. pneumoniae biochemically as bacterial species. Therefore, it is necessary to use molecular tools such as the rpoB ...
Background:. Emerging carbapenem-resistant enterobacteriacae makes it difficult to treat many gram-negative bacterial infection. We recently experienced a hospital outbreak of Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella pneumoniae (KPN) linked with an index case of community-acquired KPC-producing KPN infection. An outbreak investigation and whole genome sequencing analysis were performed to trace the outbreak and investigate the molecular characteristics of the isolates.. Methods:. Six cases of KPC-producing KPN were identified within the period October 2014 to February 2015. An epidemiological investigation and Pulsed field gel electrophoresis (PFGE) analysis showed 3 linked cases of which index case was community acquired. Active surveillance culture for exposed patients identified one more case with KPC-producing KPN colonization. Whole genome sequencing analysis for 4 linked cases were performed using a combination of Illumina Genome Analyzer (Illumina, San Diego, CA, ...
Klebsiella pneumoniae is a leading cause of severe infections in humans and dairy cows, and these infections are rapidly becoming untreatable due to the emergence of multidrug-resistant (MDR) strains. However, little is known about the relationship between bovine and human K. pneumoniae isolates at the genome population level. Here, we investigated the genomic structures, pangenomic profiles, virulence determinants, and resistomes of 308 K. pneumoniae isolates from humans and dairy cows, including 96 newly sequenced cow isolates. We identified 177 functional protein families that were significantly different across human and bovine isolates; genes expressing proteins related to metal ion (iron, zinc, and calcium) metabolism were significantly more prevalent among the bovine isolates. Siderophore systems were found to be prevalent in both the bovine and the human isolates. In addition, we found that the Klebsiella ferric uptake operon kfuABC was significantly more prevalent in clinical mastitis ...
Several bacterial populations areknownto be containing some fraction of cells which survive exposure to antibiotics and harsh environment, are called as persister cells. This fraction of cells is very small generally ranging from 10-7 to 10-5. The mechanism of persister formation is not yet clearly understood although expression of toxin-antitoxin (TA) pairs of proteins has been found to be associated with persister formation. Klebsiella pneumoniae is also shown to produce persister cells by prolonged exposure to ampicillin.In this study, we have identified a pair of proteins, hipA and hipB, of TA system in Klebsiella pneumoniae. The proteins have 70% and 60% sequence similarity respectively with their homologous proteins from E. coli. hipA and hipB associate together to regulate survival of persister cells by binding to DNA in unfavourable conditions. Both hipA and hipB proteins from Klebsiella pneumoniae were cloned, expressed and purified. The clones were over expressed in fusion with His-tag ...
Download Free Full-Text of an article RAPID DETECTION OF EXTENDED SPECTRUM ?-LACTAMASES (ESBLS) PRODUCING ISOLATES OF KLEBSIELLA PNEUMONIA BY A NEW COLORIMETRIC MEDIUM
TY - JOUR. T1 - Genomic epidemiology of Klebsiella pneumoniae in Italy and novel insights into the origin and global evolution of its resistance to carbapenem antibiotics. AU - Gaiarsa, Stefano. AU - Comandatore, Francesco. AU - Gaibani, Paolo. AU - Corbella, Marta. AU - Valle, Claudia Dalla. AU - Epis, Sara. AU - Scaltriti, Erika. AU - Carretto, Edoardo. AU - Farina, Claudio. AU - Labonia, Maria. AU - Landini, Maria Paola. AU - Pongolini, Stefano. AU - Sambri, Vittorio. AU - Bandi, Claudio. AU - Marone, Piero. AU - Sasserac, Davide. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Klebsiella pneumoniae is at the forefront of antimicrobial resistance for Gram-negative pathogenic bacteria, as strains resistant to third-generation cephalosporins and carbapenems are widely reported. The worldwide diffusion of these strains is of great concern due to the high morbidity and mortality often associated with K. pneumoniae infections in nosocomial environments. We sequenced the genomes of 89 K. pneumoniae strains ...
Klebsiella pneumoniae bacterium. Coloured transmission electron micrograph (TEM) of a section through a Klebsiella pneumoniae bacterium. K. pneumoniae is one of several bacteria that can cause pneumonia (lung inflammation). It causes a severe form that can be a complication of chronic lung diseases. K. pneumoniae is also a multidrug-resistant bacterium that can cause severe hospital-acquired infections. - Stock Image C026/3555
INCREASING INCIDENCE OF MULTIDRUG RESISTANCE Klebsiella pneumoniae INFECTIONS IN HOSPITAL AND COMMUNITY SETTINGS Journal Article published 31 Jul 2012 in International Journal of Microbiology Research volume 4 issue 6 on pages 253 to 257 Authors: SAVITA JADHAV, Department Of Microbiology, Pad. Dr. D. Y. Patil Medical College, Pimpri-411 018, Pune, MS, India, RABINDRANATH MISRA, NAGESHAWARI GANDHAM, MAHADEV UJAGARE, PURBASHA GHOSH, KALPANA ANGADI, CHANDA VYAWAHARE. ...
Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are a group of emerging highly drug-resistant Gram-negative bacilli causing infections associated with significant morbidity and mortality. Once confined to outbreaks in the northeastern United States (US), they have spread throughout the US and most of the world. KPCs are an important mechanism of resistance for an increasingly wide range of Gram-negative bacteria and are no longer limited to K pneumoniae. KPC-producing bacteria are often misidentified by routine microbiological susceptibility testing and incorrectly reported as sensitive to carbapenems; however, resistance to the carbapenem antibiotic ertapenem is common and a better indicator of the presence of KPCs. Carbapenem antibiotics are generally not effective against KPC-producing organisms. The best therapeutic approach to KPC-producing organisms has yet to be defined; however, common treatments based on in vitro susceptibility testing are the polymyxins, tigecycline, and ...
Garlic is a vegetable that is very nutritious, but also provides a huge number of health benefits. The list of diseases that garlic can kill is long, and includes: Vibrio infection Klebsiella infection HIV-1 Thrush Pseudomonas aeruginosa Mycobacterium tuberculosis, Clostridium Group B Strep Infection Cytomegalovirus Infections Helicobacter pylori infection Candida infection (yeast) Aflatoxicosis associated mycotoxins…
The most potent natural remedy against numerous health conditions is garlic, so the list of diseases that garlic can defeat is long. Here are some of them: Thrush (Fungal overgrowth in oral cavity) Mycotoxin-associated aflatoxicosis Methicillin- Resistant Staphylococcus Aureus (MRSA) HIV-1 infection Vibrio infection Klebsiella infection Helicobacter Pylori infection Candida (Yeast) infection Viral Infections (…
This SuperSeries is composed of the following subset Series: GSE35746: Comparative analysis of regulatory elements between Escherichia coli and Klebsiella pneumoniae by genome-wide transcription start site profiling [tiling arrays] GSE35821: Comparative analysis of regulatory elements between Escherichia coli and Klebsiella pneumoniae by genome-wide transcription start site profiling [TSS-Seq] Refer to individual Series
Germany has reported an outbreak of carbapenemase-producing (NDM-1 and OXA-48) and colistin-resistant Klebsiella pneumoniae sequence type (ST) 307. As of 21 October 2019, 17 patients in three hospitals and one rehabilitation clinic in Mecklenburg-West Pomerania in north-east Germany have been affected. Six of the 17 cases presented with clinical symptoms of infection, while 11 were identified as be carriers. ...
Germany has reported an outbreak of carbapenemase-producing (NDM-1 and OXA-48) and colistin-resistant Klebsiella pneumoniae sequence type (ST) 307. As of 21 October 2019, 17 patients in three hospitals and one rehabilitation clinic in Mecklenburg-West Pomerania in north-east Germany have been affected. Six of the 17 cases presented with clinical symptoms of infection, while 11 were identified as be carriers. ...
TY - JOUR. T1 - FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. AU - Hsueh, Kuang Lung. AU - Yu, Liang Kun. AU - Chen, Yung Han. AU - Cheng, Ya Hsin. AU - Hsieh, Yin Cheng. AU - Ke, Shyue chu. AU - Hung, Kuo Wei. AU - Chen, Chun Jung. AU - Huang, Tai Huang. PY - 2013/10/18. Y1 - 2013/10/18. N2 - Iron is essential for pathogen survival, virulence, and colonization. Feo is suggested to function as the ferrous iron (Fe2+) transporter. The enterobacterial Feo system is composed of 3 proteins: FeoB is the indispensable component and is a large membrane protein likely to function as a permease; FeoA is a small Src homology 3 (SH3) domain protein that interacts with FeoB; FeoC is a winged-helix protein containing 4 conserved Cys residues in a sequence suitable for harboring a putative iron-sulfur (Fe-S) cluster. The presence of an iron-sulfur cluster on FeoC has never been shown experimentally. We report that under anaerobic conditions, the recombinant Klebsiella pneumoniae FeoC (KpFeoC) ...