To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also ...
Genome duplications may have played a role in the early stages of vertebrate evolution, near the time of divergence of the lamprey lineage. Additional genome duplication, specifically in ray-finned fish, may have occurred before the divergence of the teleosts. The common carp (Cyprinus carpio) has b …
TY - JOUR. T1 - Pro- and anti-inflammatory responses of common carp Cyprinus carpio head kidney leukocytes to E.coli LPS as modified by different dietary plant oils. AU - Nguyen, Thi Mai. AU - Mandiki, Robert. AU - Salomon, Jean M. A. J.. AU - Baruti, Joel Bondekwe. AU - Thi, Nang Thu Tran. AU - Nguyen, Thu Hang. AU - Nhu, Truong Quynh. AU - Kestemont, Patrick. PY - 2021/1/1. Y1 - 2021/1/1. N2 - Dietary lipids could modify fatty acid (FA) composition in fish tissues. Long chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic acid (ARA), eicosapentaneoic acid (EPA) and docosahexaenoic acid (DHA) are able to modulate the immune status in fish through an inflammatory process but their availability may be limited when fish are exclusively fed plant oils. This study was conducted to evaluate how to maximise the utilisation of dietary plant oil for an efficient inflammatory response in common carp head kidney leukocytes (HKLs) exposed to a gram-negative bacterial endotoxin, Escherichia coli ...
Tang, Bin,Luo, Xiao-Jun,Zeng, Yan-Hong,et al. Tracing the Biotransformation of PCBs and PBDEs in Common Carp (Cyprinus carpio) Using Compound-Specific and Enantiomer-Specific Stable Carbon Isotope Analysis[J]. Environmental Science & Technology,2017,51(5):2705-2713 ...
Looking for online definition of hemoflagellates in the Medical Dictionary? hemoflagellates explanation free. What is hemoflagellates? Meaning of hemoflagellates medical term. What does hemoflagellates mean?
Alderson, G. L. H.: The development of a system of linear measurements to provide an assessment of type and function of beef cattle, Anim. Genet. Resour., 25, 45-55, https://doi.org/10.1017/S1014233900005782, 1999. Bakos, J. and Gorda, S.: Genetic improvement of common carp strains using intraspecific hybridization, Aquaculture, 129, 183-186, https://doi.org/10.1016/0044-8486(94)00245-J, 1995. Bauer, C. and Schlott, G.: Fillet yield and fat content in common carp (Cyprinus carpio) produced in three Austrian carp farms with different culture methodologies, J. Appl. Ichthyol., 25, 591-594, https://doi.org/10.1111/j.1439-0426.2009.01282.x, 2009. Bavarian State Research Center for Agriculture - Department for Quality Assurance and Analytics: Determination of intramuscular fat content in fish - modification of the VDLUFA method 5.1.1. Subject area AQU3, central laboratory, Analysis of feed and animal products, Poing, Germany, Internal documentation, received by email on 19 October 2016. Brethour, J. ...
Deoxynivalenol (DON) frequently contaminates animal feed, including fish feed used in aquaculture. This study intends to further investigate the effects of DON on carp (Cyprinus carpio L.) at concentrations representative for commercial fish feeds. Experimental feeding with 352, 619 or 953 μg DON kg−1 feed resulted in unaltered growth performance of fish during six weeks of experimentation, but increased lipid peroxidation was observed in liver, head kidney and spleen after feeding of fish with the highest DON concentration. These effects of DON were mostly reversible by two weeks of feeding the uncontaminated control diet. Histopathological scoring revealed increased liver damage in DON-treated fish, which persisted even after the recovery phase. At the highest DON concentration, significantly more fat, and consequently, increased energy content, was found in whole fish body homogenates. This suggests that DON affects nutrient metabolism in carp. Changes of lactate dehydrogenase (LDH) activity in
Feature Papers represent the most advanced research with significant potential for high impact in the field. Feature Papers are submitted upon individual invitation or recommendation by the scientific editors and undergo peer review prior to publication. The Feature Paper can be either an original research article, a substantial novel research study that often involves several techniques or approaches, or a comprehensive review paper with concise and precise updates on the latest progress in the field that systematically reviews the most exciting advances in scientific literature. This type of paper provides an outlook on future directions of research or possible applications. ...
Common carp (Cyprinus carpio) is one of the worlds most widely introduced and established freshwater fishes (Casal 2006). The species is considered to be one of the eight most invasive freshwater fishes (Lowe et al. 2000) and worldwide, it accounts for most of the records of successful establishments and adverse ecological effects (Casal 2006; Kulhanek et al. 2011). This invasive success suggests that feral C. carpio is equipped with a set of adaptable life history attributes that allow it to successfully colonise a wide range of habitats (Koehn 2004; Zambrano et al. 2006; Britton et al. 2007). Where feral C. carpio occurs in high densities, it is often perceived as an invasive pest species (Sivakumaran et al. 2003; Brown and Walker 2004; Koehn 2004) because it can have severe impacts on habitat heterogeneity and biodiversity by increasing water turbidity through its bottom feeding behaviour, increasing nutrient availability, decreasing benthic and macrophyte density and diversity, altering ...
DESIGN: The aim of these experiments was to prove increased resistance of common carp (Cyprinus carpio L.) juveniles fed on experimental diets with microalgae biomass supplement (Algadiets) to oxidative stress caused by a disinfectant chloramine-T. In indoor experiments fish were fed on laboratory-prepared extruded diets containing supplement of Chlorella spp. (cf. C. vulgaris Beijerinck) biomass which contains antioxidants (carotenoids) like lutein. The young-of-the-year-old fish were acclimatized and fed on basal diet (control group) and the on diets containing 1, 2, 5 and 10% (w/w) of spray-dried Chlorella biomass (Algadiet 1, 2, 5 and 10) for 14 days followed by 6 weeks. Consequently, fish were treated daily with chloramine-T (Chl-T) at concentration of 10 mg x l(-1) for 1 h in three consecutive days. After this treatment, the indices of oxidative stress and antioxidant enzyme activity were assayed in fish gill, muscle and hepatopancreas ...
Localization, expression and control of adrenocorticotropic hormone in the nucleus preopticus and pituitary gland of common carp (Cyprinus carpio L ...
Charvatova N, Zelinska G, Dobsikova R, Stancova V, Zivna D, Plhalova L, Blahova J, Sehonova P, Marsalek P, Bartoskova M, Prokes M, Piskorova I, Svobodova Z. The effect of the fluoroquinolone norfloxacin on somatic indices and oxidative stress parameters in early stages of common carp (Cyprinus carpio L.). Neuro Endocrinol Lett. 2015 Jan; 36(Suppl 1): 79-87 ...
Examination of common carp Cyprinus carpio, carrying implanted telemetry transmitters, showed that wound healing was incomplete after 4 months and completed after 1 year. Granulation tissue, which encapsulated the transmitter, adhered to the intestine and liver. The liver was infiltrated by the granulation tissue after 1 year. Epidermal tissue grew down the channel for the antenna, establishing a potential pathway for pathogens. No signs of infections, however, were found ...
Trypanosoma brucei, the causative agent of African sleeping sickness, evades the immune response by expressing a coat of variant surface glycoprotein (VSG). VSG is expressed from a single telomeric expression site (ES), along with a number of expression site associated genes (ESAGs). Thus far, the function of most ESAGs is unknown. One ES contains the serum resistance associated gene (SRA), which confers resistance to trypanosome lytic factor in T. b. rhodesiense. Only three other ESAGs -5, 6 and 7 - are present in this ES. ESAGs 6 and 7 encode a heterodimeric transferrin receptor, but the function of ESAG5 has not been identified. We present here a bioinformatic analysis of ESAG5 and distinguish between T. brucei-specific ESAGs and Genes Related to ESAG5 (GRESAGs), which occur outside of ESs in chromosomal-internal contexts. Further, a genome-wide survey of these genes across kinetoplastids identifies a family of GRESAG5s in a number of species. Analysis of phylogenetic relationships indicates that
Trypanosoma brucei, the causative agent of African sleeping sickness, evades the immune response by expressing a coat of variant surface glycoprotein (VSG). VSG is expressed from a single telomeric expression site (ES), along with a number of expression site associated genes (ESAGs). Thus far, the function of most ESAGs is unknown. One ES contains the serum resistance associated gene (SRA), which confers resistance to trypanosome lytic factor in T. b. rhodesiense. Only three other ESAGs -5, 6 and 7 - are present in this ES. ESAGs 6 and 7 encode a heterodimeric transferrin receptor, but the function of ESAG5 has not been identified. We present here a bioinformatic analysis of ESAG5 and distinguish between T. brucei-specific ESAGs and Genes Related to ESAG5 (GRESAGs), which occur outside of ESs in chromosomal-internal contexts. Further, a genome-wide survey of these genes across kinetoplastids identifies a family of GRESAG5s in a number of species. Analysis of phylogenetic relationships indicates that
Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant
Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM+ B cells, because after four days of stimulation the IgM+ fraction of carp kidney and s ...
TY - JOUR. T1 - Molecular and biochemical studies on the hypoxanthine-guanine phosphoribosyltransferases of the pathogenic haemoflagellates. AU - Ullman, Buddy. AU - Carter, Darrick. N1 - Funding Information: Acknowledgemenrs-This work was supported by grant A I-23682 from the National Institute of Allergy and Infectrouh Disease. Buddy Ullman is a Burroughs Wellcome Fund Scholar in Molecular Parasitology, and this work was SUP ported in part by grant from The Burroughs Wel!cnme Fund.. PY - 1997/2. Y1 - 1997/2. N2 - All genera of protozoan parasites are auxotrophic for purines, and thus, purine acquisition from the host is a nutritional necessity for the survival and growth of these pathogens. Many of these parasites, including Trypanosoma brucei, Trypanosoma cruzi and Leishmania spp., access host purines by phosphoribosylating purine bases via purine phosphoribosyltransferase (PRT) enzymes. The trypanosomatid hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme has been implicated as a ...
Legal action in the UK has driven Shell to make an out-of-court settlement of £55 million (CAD$83 million) to compensate the Bodo community of Nigeria after the livelihoods of thousands were destroyed by oil spills. The £55 million will be split between £35 million for 15,600 individuals and £20 million for the community.. Shells long-overdue compensation payout is an important victory for the victims of corporate negligence.. What happened?. On August 28, 2008, a fault in the Trans-Niger pipeline caused a significant oil spill into Bodo Creek in Ogoniland, Nigeria. The pipeline is the responsibility of Shell. The spill, which was due to equipment failure, resulted in tens of thousands of barrels of oil polluting the land and creek surrounding Bodo, killing the fish that people depend on for food and livelihood. A second major spill began on December 7, 2008.. How did Amnesty supporters make a difference?. Amnesty International and the Centre for Environment, Human Rights and Development ...
Nagradna igra traja od 27.7.2017 do 31.7.2017 do 24.00. Žreb bo izveden 1.8.2017 in nagrajenci bodo v najkrajšem roku po elektronski pošti obveščeni, da naj sporočijo svoje ime in priimek ter naslov. Rok za sporočilo imena in priimka ter naslova, če je ta potreben, je do 4.8.2017 do 24.00. V primeru, da se nagrajenci v roku ne bodo oglasili bo izveden ponovni žreb.. Posredovani podatki se ne hranijo. Organizator si pridržuje pravico do enostranskega spreminjanja splošnih pogojev nagradne igre. Vsaka sprememba bo objavljena na strani nagradne igre. Nagradna igra poteka na ozemlju Republike Slovenije. Sodelovanje v nagradni igri pomeni, da izrecno in nedvoumno sprejemate pravila nagradne igre. Organizator ima pravico, da lahko kadarkoli prekine nagradno igro pred njenim zaključkom v primeru dogodkov, na katere nima vpliva (višja sila), v primeru materialne zlorabe ali kršitve pravil. Vsi udeleženci bodo o tem obveščeni na spletni strani nagradne igre. V tem primeru udeleženci ...
Trypanosomatids of the order Kinetoplastida are major contributors to global disease and morbidity, and understanding their basic biology coupled with the development of new drug targets represents a critical need. Additionally, trypanosomes are among the more accessible divergent eukaryote experimental systems. The genome of Trypanosoma brucei contains 8,131 predicted open reading frames (ORFs), of which over half have no known homologues beyond the Kinetoplastida and a substantial number of others are poorly defined by in silico analysis. Thus, a major challenge following completion of the T. brucei genome sequence is to obtain functional data for all trypanosome ORFs. As T. brucei is more experimentally tractable than the related Trypanosoma cruzi and Leishmania spp. and shares |75% of their genes, functional analysis of T. brucei has the potential to inform a range of parasite biology. Here, we report methods for systematic mRNA ablation by RNA interference (RNAi) and for phenotypic analysis,
FIG. 1. Stability of PFR2 mRNA in promastigotes and amastigotes. Promastigotes and amastigotes were treated with actinomycin D (10 μg/ml) for the time indicated. Amastigotes received additional actinomycin D at 1 h. The zero time point was taken just prior to the addition of actinomycin D. Five micrograms of total RNA from each time point was fractionated, blotted, and probed with in vitro-synthesized RNA from pPFR2 as described in the text. (A) Representative Northern blots for the promastigote and amastigote time courses. The signals for the the PFR2 and rRNA probes are displayed. (B) PFR transcript abundance was quantitated by using a phosphorimager and was normalized to the small subunit of rRNA to control for loading. For each time point, the normalized values for PFR2 mRNA ([R]t) were divided by the normalized time zero value ([R]0) to obtain a value for the fraction of PFR2 mRNA remaining ([R]t/[R]0) which was plotted against time, in hours. The plotted data were fitted to the ...
The glycosome is a membrane-enclosed organelle that contains the glycolytic enzymes. The term was first used by Scott and Still in 1968 after they realized that the glycogen in the cell was not static but rather a dynamic molecule. It is found in a few species of protozoa including the Kinetoplastida which included the suborders Trypanosomatina and Bodonina, most notably in the human pathogenic trypanosomes, which can cause sleeping sickness and Chagass disease, and leishmaniasis. The organelle is bounded by a single membrane and contains a dense proteinaceous matrix. It is believed to have evolved from the peroxisome. This has been verified by work done on Leishmania genetics. The glycosome is currently being researched as a possible target for drug therapies. Glycosomes are unique to kinetoplastids. The term glycosome is also used for glycogen-containing structures found in hepatocytes responsible for storing sugar, but these are not membrane bound organelles. Glycosomes are composed of ...
Our research is aimed at understanding the molecular mechanisms of RNA editing in kinetoplastid parasites, which cause African sleeping sickness, Chagasdisease...
Definition: Living in a fluid medium (eg: water) but unable to maintain their position or distribution independently of the movement of the fluid (adapted from Lincoln et al., 1998 ...
Poslušanje podcastov je odličen način, da se izobrazite o številnih vidikih teme las. Taylor Campbell je za vas naštela 20 zanimivih podcast las. Obstajajo oddaje za frizerje, ženske z naravnimi lasmi in lepotne navdušence.
hallo out there, have anybody knowledge of RNA editing in trematodes or other parasites (without kinetoplastida)? thanks, --------------------------------------------- , Peter Schuessler , , Dep.: Genetic Parasitology , , Institute for Genetic , , Heinrich-Heine-University Duesseldorf , , Universitaetsstr.1 , , D-40225 Duesseldorf , , Germany , , , , Tel: ++49-(0)211-811-2408 , , Fax: ++49-(0)211-811-2333 , , e-mail: schuessl at uni-duesseldorf.de ...
This question is very important for the entertainment industry: does it matter whether youre right, when youre telling a story to entertain? Under some circumstances, I dont think it matters. But with an animated movie about real, living systems, when you use the truth - their complexity and beauty - as a springboard for the story, you add a level of gravitas that is vitally important to creating a broad and deep appeal. A young audience is much more sophisticated than you think, and a story informed by a lot of facts alerts them to the presence of real concepts. I got an e-mail from an eight-year-old about Finding Nemo, explaining that characters could not emerge from a whales blowhole if they were in its mouth, because there is no link between the trachea and the oesophagus. ...
A composition and method for applying an electrically conductive coating to an initially electrically nonconductive surface, such as a printed wiring board having through holes is disclosed. The method is carried out by applying a composition of electrically conductive carbon particles, a second conductive material, a water dispersible binding agent, and an aqueous dispersing medium to a nonconductive substrate to form a substantially continuous, electrically conductive coating. Enough of the carbon particles are present to provide an electrically conductive coating when the composition is applied to the substrate. Enough of the second conductive material is present to provide an improved electrically conductive coating when the composition is applied to the substrate. The resulting coating carbon coating has an improved conductivity over prior through hole coating compositions and processes and is capable of being exposed to molten solder without resulting in the formation of blowholes.
Where once had stood the Kings royal row of artisans and sages, there were now only ruined husks. The line of beautiful buildings had once stretched like an emerald necklace all the way to the Chapel of Tears. Now they looked like rotten gums, cracked from the brutality of their broken teeth. They were bleeding calcium stumps; tainted with the untreatable infection of desperate people in desperate circumstances. Even the Chapel of Tears, an indestructible building carved and dried from the carcass of a yellow whale that had brought the Kings people to this land in its belly, was sunken into a deflated lump. No incense rose from the blowhole in supplication to the gods of under and above. The pair of steel mesh cages which functioned as its doors hung wide open on sprung hinges, displaying unadulterated emptiness. There werent even any nesting birds or shivering dogs to worship inside its lifeless heart ...
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Quote: The bezel is made of a thick molded polystyrene (the stuff model cars are made of, but much thicker). The bolts are molded into the bezel and have a chrome finish. The LCD display is a temp monitor. As you can see, the case doesnt have a blowhole. The top is held by screws instead of pop rivets, so cutting
By Lizette Borreli via @medicaldaily.com Spring is a time to celebrate the miracle of life and reflect on not just how mothers have impacted our lives, but how motherhood has impacted them. During the course of nine months of pregnancy, moms-to-be undergo common physical and emotional changes that lead to childbirth, from weight gain to mood swings. However, Healthline.coms infographic &ld...
Vaccines from AstraZeneca, Russias Gamaleya Institute and Johnson & Johnson fight the coronavirus with another virus, leaving scientists concerned the shots may lose potency if annual inoculations become necessary to fight new variants. Most vector-vaccine developers have opted to use an adenovirus, a harmless class of common-cold viruses.The experience with adenoviruses has been for many years that vectors can be intercepted by the immune system after repeat injections, said Bodo Plachter, deputy director of the Institute of Virology at Mainz Universitys teaching hospital.
Spletno mesto uporablja piškotke. Nekatere piškotke, ki so nujni za delovanje spletne strani, smo že nastavili. Uporabljamo Google Analytics, ki uporablja piškotke. Pobrišete ali blokirate lahko vse piškotke iz te spletne strani, vendar deli spletne strani ne bodo delovali pravilno. Za podrobne informacije o piškotkih si preberite politiko uporabe.Strinjam se z uporabo piškotkovO piškotkih in njihovi uporabi na naši spletni strani ...
Ta spletna stran uporablja piškotke med vašim obiskom spletne strani. Piškotki, ki so potrebni za delovanje spletne strani se shranijo v vaš brskalnik saj so ključni za delovanje osnovnih funkcionalnosti spletnega mesta. Uporabljamo tudi piškotke drugih proizvajalcev, ki nam pomagajo analizirati in razumeti kako uporabljate to spletno mesto. Ti piškotki bodo shranjeni samo z vašim soglasjem. Prav tako imate možnost, da se od teh piškotkov odjavite, toda izklop nekaterih piškotkov lahko vpliva na vašo uporabniško izkušnjo ...
Nova avtorska oddaja nas bo popeljala po Sloveniji! S prikupno voditeljico Ingrid Ulaga do gledalci v zabavnem popotniškem raziskovalnem kvizu odkrivali slovenske kraje, spoznavali naravne znamenitosti in zanimivosti, krajevno zgodovino, kulturno in etnološko dediščino, turistično ponudbo, legende in mite, zanimive sogovornike, hkrati pa boste reševali uganke, povezane s konkretnim krajem … V vsaki oddaji bodo gledalci z voditeljico iskali ključ z uganko, ki bo vsak teden pripeljal do nagradnega gesla. Voditeljico Ingrid Ulaga gledalci TV3 že dobro poznajo. Lani je vodila oddajo Moja pesem, tvoj glas, v kateri so znani slovenski pevci in pevke peli priredbe svojih kolegov, več sezon pa je vodila oddajo Odtenki slavnih, v kateri je razkrivala podrobnosti iz življenja zvezdnikov. Ingrid je z novim izzivom več kot zadovoljna: Nova oddaja je popolnoma nov izziv zame. Ne samo, da oblačila niso več studijska (oblečena sem v stilu Indiana Jones), tudi tematika je popolnoma druga - meni ...
10.in 11. novembra 2017 je bilo v Hotelu Convent in v OBV srečanje specializantov in mladih specialistov ortopedije iz cele Evrope. Prišli so predstavniki več kot 12 evropskih držav. Dogodek je bil predvsem operativne narave, analizirali smo delovanje poletne šole za specializante na Portugalskem in pripravili izhodišča za poletno šolo prihodnje leto v Milanu. Nadgradili smo tudi vzpostavitev mreže učnih centrov za fellowshipe po vsej Evropi, ki bodo prihodnjim mladim kirurgom omogočili učinkovito učenje in pridobivanje veščih. Vsi udeleženci so bili neizmerno navdušeni nad našo bolnišnico, ki si tako širi prepoznavnost in mednarodni ugled tudi pri mlajših generacijah, kar je dobra popotnica za prihodnost.. Poročilo prispeval Anže Mihelič, dr. med., spec. ortoped. ...
Pri arterijski hipertenziji in kroničnih oblikah srčnega popuščanja je potrebno jemati tablete Losartan - navodila za uporabo bodo razložila, kak
Schubach, Armando; Barros, Mônica Bastos de Lima; Schubach, Tânia Maria Pacheco; Francesconi-do-Valle, Antônio Carlos; Gutierrez-Galhardo, Maria Clara; Sued, Márcio; Salgueiro, Mariza de Matos; Fialho-Monteiro, Paulo Cezar; Reis, Rosani Santos; Marzochi, Keyla Belizia Feldman; Wanke, Bodo; Conceição-Silva, Fátima Less ...
Battlefront 2 je ob izidu terjala ceno okoli 79,90€ za osnovno izdajo. Vendar ta znaten vložek ni dovolj, če želite iz igre iztisniti kar največ in vseeno ohraniti nekaj prostega časa. Založnik, Electronic Arts, je v igro vprogramiral nadležen sistem napredovanja, ki se zanaša na Zvezdne karte, s katerimi je moč izboljšati like, ladje in orožja. V igri je na ducate različnih kartic, od najbolj vsakdanjih, ki vplivajo le na izgled lika, do najbolj redkih, ki znatno izboljšajo moč orožja in plovil, s katerimi se igralec bojuje. Boljše so karte, s katerimi igralec razpolaga, boljše bodo možnosti za uspeh na bojišču. Te karte je moč kupiti z novci, ki jih vsak vojak zasluži v večigralskih bitkah. Seveda pa jih je bilo moč kupiti tudi s težko prisluženimi evri, kar je igralcem skočilo v nos. Najbolj podjetni so hitro ugotovili, da je potrebnih 40 ur igranja, če želite odkleniti lik Dartha Vaderja. To zveni bolj kot delovni teden in manj kot zabavno preživljanje ...
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Oksidativne poškodbe in neprimerno delovanje mitohondrijev je značilno za Parkinsonovo bolezen, Alzheimerjevo bolezen in Friedreichovo ataksijo. Predklinična študija na 80 ljudeh (dvojno slepa, randomizirana študija kontrolirana s placebom) je pokazala, da lahko CoQ10 ščiti nigrostriatni dopaminergični sistem in velike doze (1200 mg/dan) CoQ10 skozi 16 mesecev naj bi upočasnile napredovanje PB. Neka druga študija z 360 mg dnevno je prav tako zaznala izboljšanje.[10] Vendar pa so potrebne še večje študije, ki bodo te izsledeke potrdile in ovrednotile pozitvne učinke. Številne klinične študije CoQ10 v zadnjih letih v glavnem podpirajo njegov nevroprotektiven efekt. Pomembno CoQ10 varuje človeške nevroblastomske celice. Pomanjkanje kompleksa I in II v kožnih fibroblastih pri Parkinsonovi bolezni se izboljša s CoQ10. Pri Friedrichovi ataksiji s sukcinatom reduciran CoQ10 varuje membranske lipide in kompleks II pred poškodbo železa v homogenatu človeškega srca in vitro. ...
were missing out on something very important. an Adivasi girl from Gaurinagar, is staying with Bodo girls After the exams end these girls dont want to go home We want to study more I want to become a school teacher said Elina Given this enthusiasm among the girls Nedan Foundation is organising a month-long counseling for them after the exams are over For all the latest India News download Indian Express App More Related NewsBy: Express News Service , Kolkata , Published: September 12 2016 12:52 am Madan Mitra was released from Alipore jail early on Saturday and taken to a hotel within the Bhawanipore police station limits Top News A day after he was released on bail Saradha scam accused and Trinamool Congress leader Madan Mitra complained of chest pain and breathlessness on Sunday morning According to a source close to the former state transport minister he began to feel uneasy after having breakfast A doctor was called to attend to him at the south Kolkata hotel where he is staying Mitra ...
Društvo MDS je 23. marca pristopilo k organiziranemu zbiranju pooblastil za skupščino družbe Center naložbe, d.d., ki bo potekala jutri, v petek, 2. aprila ob 13. uri. Pooblaščenci Društva MDS so zbrali skupaj več kot 42.500 glasov, kar predstavlja več kot 1,1 % kapitala družbe.. Hkrati velja poudariti, da ima družba Center naložbe preko 30.000 delničarjev, zaradi česar je Društvo MDS pristopilo k omejenemu zbiranju pooblastil, zaradi omejenih finančnih zmožnosti. Pred samo skupščino bo ob 11.00 uri v CITY Hotelu potekala tudi tiskovna konferenca Društva MDS, kjer bodo med drugim izpostavljene tudi povezave med družbami Pivovarna Laško, Infond Holding in Center naložbe.. ...
Leta 1240 je Polock postal vazal litovskih knezov. Veliki litovski knez Vitenis je leta 1307 na silo priključil Polock k svoji kneževini in končal proces litovskih osvajanj, ki so se začela v 1250. letih.[6] Mesto je dobilo listino z garancijami, da veliki knezi ne bodo uvedli nič novega in ne uničili starega.[7] Litovcem je s takšno politiko uspelo pridobiti trgovsko pot po reki Dvini, ki je postala pomemben gospodarski dejavnik.[6] Leta 1498 je bil sprejet Magdeburški zakon, s katerim je Polock postal do leta 1772 prestolnice Polockega vojvodstva v Republiki obeh narodov. Leta 1563 ga je zasedla ruska vojska Ivana Groznega. Po petnajst letih ga je Rusija vrnila Republiki obeh narodov. 17. junija 1654 ga je ponovno okupirala Rusija. Med rusko-poljsko vojno (1654-1667) je 30. oktobra 1660 ponovno prišel pod poljsko-litovsko oblast. V prvi delitvi Poljske je Polock leta 1773 dobila Rusija ruski del Republike obeh narodov. Ker ruska carica Katarina Velika ni priznala papeškega ...
Šesto množično izumrtje je na obzorju in zanj, kot lahko spet ugotovimo s temi našimi možgani, smo odgovorni v veliki meri kar mi sami. Posledice delovanja naših možganov in nadaljnjo evolucijo naše vrste je v svojem romanu Galapagos skoraj optimistično opisal Kurt Vonnegut - ko bomo uspeli vse uničiti, bo naša telesa počasi spet začela preraščati dlaka, udi se nam bodo spremenili v plavuti in srečno bomo zaplavali v kraj našega izvora - v oceane.. In z razvojem civilizacije smo prišli tudi do človekovih pravic. Tudi te nas ločujejo od drugih živali. Postavili smo si določene norme, nekatere smo zapisali, s predvidevanjem, da jih bomo tudi upoštevali. Človekove pravice nimajo ničesar s preživetjem vrste, imajo pa vse s tem, kako danes razumemo svet, v katerem živimo. Svet, ki ga ne obvladuje le naš plazilski del možganov, ki smo ga podedovali od plazilcev in ti od rib in te od prve celice, ki je bila sposobna zaznavati svet okrog sebe, ampak ta novi del možganov, ki ...
Procesorska osnova RISC-V je odprtokodna. To pomeni, da bodo novi procesorji podjetja Imagination Technologies združljivi z bogato paleto obstoječe strojne opreme.
Fulltext - Growth Performance and Health Status of Common Carp (Cyprinus carpio) Supplemented with Prebiotic from Sweet Potato (Ipomoea batatas L.) Extract
In order to elucidate the importance of food-borne chemical contamination in fish, cytological and ultrastructural alterations in hepatocytes and enterocytes of common carp Cyprinus carpio L. exposed for 5 wk to 0.5 microgram endosulfan (6,7,8,9,10,1
magnetic field was generated with a cylindrical coil of 80 cm length, an inner diameter of 18.5 cm and an outer diameter of 20 cm; coil constisted of 490 turn of enameled copper wire (2.5 mm diameter); fish were placed in the middle of the coil to ensure that all fish received th same magnetic field intensity; coil was positioned in an east-west direction and magnetic field was parallel to long axis of fish body; digital temperature controller has been installed inside the coil; an air conditioning system ventilated the coil interior chamber to maintain temperature at 22°C; plastic pipes containing cold tap water which circulated around the coil were used for cooling ...
The common carp (Cyprinus carpio L.) is one of the major aquaculture species, contributing nearly 35% to the inland fish production in Karnataka, India. Stocks collected from Hungary (2), Indonesia and Vietnam were assessed alongside two local stocks in a series of culture performance trials with the objective of setting up a base population for developing a breeding programme. The present study deals with the genetic divergence and polymorphism in these six stocks using random-amplified polymorphic DNA (RAPD) markers. A total of 180 decamer random primers were screened for polymerase chain reaction (PCR) amplification (OPA 1-20, OPB1-20, OPC1-20, OPD1-20, OPE1-20, OPF1-20, OPG1-20, OPP1-20 and OPM1-20). Eight primers were selected for analysis of common carp genotypes (OPA-7, OPA-20, OPB-17, OPF-10, OP F-9, OPG-4, OPG-9 and OPP-16). Out of 492 bands recorded, 57.1% were polymorphic. Stepwise regression analysis was carried out to find best combination markers affecting body weight (P , 0.001). ...
Three embryonic class II myosin heavy chains (MYHs) were cloned from the common carp (Cyprinus carpio L.), MYHemb1,MYHemb2 and MYHemb3. MYH DNA clones were also isolated from the slow muscle of adult carp acclimated to 10°C (MYHS10)and 30°C (MYHS30). Phylogenetic analysis demonstrated that MYHemb1 and MYHemb2 belonged to the fast skeletal muscle MYH clade. By contrast, the sequence of MYHemb3 was similar to the adult slow muscle isoforms, MYHS10 and MYHS30. MYHemb1 and MYHemb2 transcripts were first detected by northern blot analysis in embryos 61 h post-fertilization (h.p.f.) at the heartbeat stage, with peak expression occurring in 1-month-old juveniles. MYHemb1 continued to be expressed at low levels in 7-month-old juveniles when MYHemb2 was not detectable. MYHemb3transcripts appeared at almost the same stage as MYHemb1transcripts did (61 h.p.f.), and these genes showed a similar pattern of expression. Whole mount in situ hybridization analysis revealed that the transcripts of MYHemb1 and ...
And in addition a quick google search actually bought a bunch of research on this up.. http://www.sciencedi...016648083710099. Growth Hormone and Gonadotropin Secretion in the Common Carp (Cyprinus carpio L.): In Vitro Interactions of Gonadotropin-Releasing Hormone, Somatostatin, and the Dopamine Agonist Apomorphine. http://www.springerl...11880540350234/. Characterization of growth hormone binding sites in the goldfish,Carassius auratus: effects of hypophysectomy and hormone injection. A recombinant carp growth hormone (rcGH) was used to develop for a GH radioreceptor binding assay in the goldfish (Carassius auratus). Specific binding of125I-rcGH to goldfish liver membranes was a pH, time, temperature, and membrane protein dependent process.. (Use of artificially manufactured carp GH -rcGH- on Goldfish shows that goldfish have receptors for GH. Its a cloned GH, to whoever was asking before. There are MANY of them out there.. Stone, N., Mc Nulty, E. & Park, E. (2002) The Effect of Stocking and ...
2 Sari Agricultural Sciences and Natural Resources University (SANRU), Faculty of Animal Science and Fisheries, Department of Fisheries, Sari, Iran DOI : 10.4194/1303-2712-v18_4_11 Viewed : 3855 - Downloaded : 3082 This study examined the effects of Immunowall® prebiotic and Primalac® probitic (each with 1.0 and 1.5 g kg−1 diet, respectively) on common carp (Cyprinus carpio L.) fry (n= 4200, 10 ± 0.015 g) from the Caspian Sea. Specific growth rate (SGR), body weight gain (BWG), total length (TL), survival, feed intake (FI), feed conversion ratio (FCR), carcass composition, and hematological parameters were evaluated in triplicate treatments after 8 weeks. BWG, SGR, TL, and survival rate were significantly influenced in the fish fed 1.5 g of mixed Immunowall® and Primalac® ( ...
Epidermal mucus (mucus) is increasingly applied to fish ecological studies based on stable isotope analysis (SIA) due to its non-invasive collection. However, knowledge on mucus SI turnover rates of individual fish remains limited, including uncertainty over how they are influenced by fish body sizes. Here, a diet switch experiment predicted mucus SI turnover rates (δ13C and δ15N) as a function of time using samples taken over 200 days from 10 individually tagged common carp Cyprinus carpio covering two size groups. Non-linear mixed effects models revealed rapid turnover of both δ13C and δ15N (T50: 2-5 days; T95: 9-22 days); δ15N turnover rates were slower for the larger cohort, while δ13C turnover rates were independent of body size. Within size groups, turnover rates were not expected to vary between individuals. These experimental results suggest that due to these fast turnover rates, epidermal mucus can provide insights into the diets of fish over very short timeframes, although for ...
Corcoran J, Lange A, Cumming RI, Owen SF, Ball JS, Tyler CR, Winter MJ. (2014) Bioavailability of the imidazole antifungal agent clotrimazole and its effects on key biotransformation genes in the common carp (Cyprinus carpio), Aquat Toxicol, volume 152, pages 57-65, DOI:10.1016/j.aquatox.2014.03.016. [PDF ...
Kinetoplastid parasites, including Trypanosoma brucei, Trypanosoma cruzi, and Leishmania spp., are transmitted by insect vectors and infect 20 million people, mainly in the most impoverished regions of the world. T. brucei is the causative agent of human African trypanosomiasis, which is a health threat to millions in sub-Saharan Africa and is 100% fatal if left untreated (1). Investigations into the novel biology of kinetoplastids may provide new chemotherapeutic avenues. Indeed, these early-branching eukaryotes utilize unusual processes for gene expression regulation and metabolic organization, and a large number of predicted kinetoplastid proteins lack any homology to proteins in their mammalian hosts.. Kinetoplastids are named for their distinctive mitochondrial DNA network, known as a kinetoplast, or kDNA, localized within their single mitochondrion. In T. brucei, kDNA is a giant catenated network of circular molecules comprised of a few dozen copies of a maxicircle (∼23 to 40 kb) and ...
The mechanisms by which proteins are targeted to the membrane of eukaryotic flagella and cilia are largely uncharacterized. We have identified a new family of small myristoylated proteins (SMPs) that are present in Leishmania spp and related trypanosomatid parasites. One of these proteins, termed SMP-1, is targeted to the Leishmania flagellum. SMP-1 is myristoylated and palmitoylated in vivo, and mutation of Gly-2 and Cys-3 residues showed that both fatty acids are required for flagellar localization. SMP-1 is associated with detergent-resistant membranes based on its recovery in the buoyant fraction after Triton X-100 extraction and sucrose density centrifugation and coextraction with the major surface glycolipids in Triton X-114. However, the flagellar localization of SMP-1 was not affected when sterol biosynthesis and the properties of detergent-resistant membranes were perturbed with ketoconazole. Remarkably, treatment of Leishmania with ketoconazole and myriocin (an inhibitor of ...
Fresh plasma from Cryptobia-resistant brook charr ( Salvelinus fontinalis) lysed Cryptobia salmositica under in vitro conditions. However, the parasite was not lysed if the plasma was heat-inactivate
Organic selection should favour parents that are able to adjust their offsprings life-history strategy and resource allocation in response to changing environmental and sociable conditions. in acquisition of sociable dominance, which in natural systems would reduce access to mating opportunities. In doing so, however, they steer clear of the reduced disease resistance associated with improved testosterone and dominance. The benefits of accelerated clearance of illness and reduced weight loss during illness may outweigh costs associated with reduced social dominance in an environment where the risk of disease is definitely high. Dawkins [46]) across two sponsor generations. In contrast, however, mite-infected female lizards (varieties are tick-borne haemoprotozoan parasites that infect virtually all mammalian varieties, with significant economic consequences in home animals and human being health implications, particularly in the USA [52,53]. In mice, induces high but transient parasitaemias, ...
|ns4:p||ns4:bold|Background:|/ns4:bold| Central to the successful elimination of |ns4:italic|Plasmodium falciparum|/ns4:italic| malaria, are tests with superior capability of diagnosing low-density parasitaemias. Empirical evidence on the performance of the commonly available diagnostics (light microscopy (LM), rapid diagnostic tests (RDT) and polymerase chain reaction (PCR)) is needed to better inform case management and surveillance activities within primary health care settings where elimination of |ns4:italic|falciparum|/ns4:italic| malaria is targeted. The objective of this study was to estimate the sensitivity (Se) and specificity (Sp) and predictive values of LM, RDT and PCR tests for |ns4:italic|P. falciparum|/ns4:italic| infection in children, while evaluating the effect of specific covariates on the accuracy of the tests.|/ns4:p||ns4:p| |ns4:bold|Methods:|/ns4:bold| The study enrolled 1,563 children presenting with fever (axillary temperature ≥ 37.5|ns4:sup|0|/ns4:sup|C) to the Ngerenya
Its a great and legit on-line dating service to satisfy your love at. Thanks to this site, millions of customers have been fortunate sufficient to show their personal lives round! Become a Premium person and get entry to all services. The variety of users in Argentina and neighboring countries recommends this dating web site. LatinAmericanCupid.com has a huge number of profiles that may be browsed at any time.. Their love bloomed unbeknownst to their family members. When they finally gave into ardour they got caught and Emily was forced to walk away from her love. Alej was misled and assume Emily was a classy bi&% and he channeled his hatred into changing into a Billionaire. However, once more not wanting to give him the higher hand by telling him the reality, she lets him assume its his cash that swayed her into accepting his choice. He tells her that she is just is short-term bride and nothing extra. Shed stopped courting males as a result of she at all times in contrast them to Alej and ...
Cadmium, Inhibin, Cleft Lip, Cleft Palate, Costs, Emotional Stress, Families, Health, Lip, Palate, Patients, Public Health, Risk, Affect, Annexin V, Anti-mullerian Hormone, Blood, Blood-testis Barrier, Cadmium Chloride, Cells
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Leishmania are protozoan parasites that fall under the genus Leishmania and the order Kinetoplastida. This parasite causes the vector borne disease known as leishmaniasis.
This dataset accompanies a manuscript describing a method to predict host-pathogen protein interactions using structure: Host-pathogen protein interactions predicted by comparative modeling. Davis FP, Barkan DT, Eswar N, McKerrow JH, Sali A. Protein Sci (2007) 16:2585-2596. http://www.proteinscience.org/cgi/doi/10.1110/ps.073228407 The files contain predictions made for 10 human pathogens including species of Mycobacterium, Apicomplexa, and Kinetoplastida. The species.all.zip files contain all interactions predictions for each species along with the filter criteria that each interaction passed. The species.filter.zip files contains the same information, but only for the subset of interactions that passed the biological and network-level filters. These files can be viewed in any spreadsheet program, such as Excel. Predictions were made for interactions between human and Mycobacterium tuberculosis: mtuber Mycobacterium leprae: mleprae Leishmania major: lmajor Trypanosoma brucei: tbrucei Trypanosoma
چکیده مطالعة حاضر با هدف تعیین تأثیرات جانشینی روغن ماهی با روغن گیاهی کلزا در جیرة غذایی در عملکرد رشد، کارایی تغذیه‌ای و شاخص اقتصادی جیرة غذایی ماهیان جوان کپور معمولی (Cyprinus carpio) طراحی شد. در این آزمایش 270 قطعه کپور معمولی جوان با وزن اولیة 10/0±24/16 گرم به صورت کاملاً تصادفی در 18 تانک استوانه‌ای پلی‌اتیلنی 300 لیتری توزیع شد. ماهیان کپور معمولی جوان با شش جیرة غذایی با سطوح یکسان پروتئین و انرژی و با شش سطح جانشینی روغن ماهی (Fish oil, FO) با روغن کلزا (Canola oil, CO) به ترتیب تیمار اول 100 درصد روغن ماهی و 0 درصد روغن کلزا (100FO)، تیمار دوم حاوی 80 درصد روغن ماهی و 20 درصد
در این تحقیق اثر پوست سبز گردو بر روی رنگ ماهی کوی(Cyprinus carpio) مورد مطالعه قرار گرفته است. تعداد 60 عدد ماهی کوی با میانگین وزن 06/0 ±50/1 گرم و میانگین طول 48/1±00/53 میلی متر در قالب 3 تیمار و یک گروه شاهد و هر کدام با سه تکرار تقسیم شدند. 12 عدد آکواریوم به ابعاد 40×33×50 سانتی متر آماده و 5 قطعه ماهی به هر کدام اضافه شد. گروه شاهد با غذای فاقد افزودنی (غذای پایه) تغذیه گردید. تیمار اول با غذای حاوی 1/0 درصد، تیمار دوم با 2/0 درصد و تیمار سوم با غذای حاوی 3/0 درصد پودر سبز گردو تغذیه گردیدند. مدت 8 هفته (56 روز) دوره پرورش به طول انجامید. هر 20 روز یک بار زیست‎سنجی شامل وزن سنجی و طول سنجی
(youtube) While on a whale watching cruise in Nova Scotia, it was not until I watched the video much later that I saw the spray of the whale closest to the boat catch the sun and turn the spray into a rainbow. It looks as if the whale is blowing a rainbow out of its blowhole.
Beatiful, but tough course. The course is an almost entirely flat three looper with a couple of small inclines that allow you to stand to stretch the legs. The first section runs south through a typical Carribean vegetation - palm trees, tall shrubs, etc. This section as almost completely shielded from the wind and allowed for a good pace - I averaged around 20-21 mph here holding back. Around 6-7 miles or so, the road turns and runs southeast and eventually east into some strong headwinds coming from the east ocean side of the island. The size of the vegetation slowly decreases and the wind picks up the closer you get. So after a few miles riding into a headwind, you turn for a 13 mile ride north in a strong ocean crosswind. The coastline is absolutely beautiful - totally undeveloped beaches, blowholes, rocky terrain - something I appreciated less and less on my second and third lap compared to lap 1. Here, the road meets the sand and their is almost no vegetation whatsover to break up the ...
Venture beneath towering crags and up close to the awe-inspiring Breathing Rock blowhole. You will enter deep sea caves, pass through the narrow gap between the coast and The Monument and search for abundant wildlife including seals, dolphins, migrating whales and sea birds.
Living with oil spill in Ogoniland . Posted 18 September 2011, by George Onah,Vanguard Media, vanguardngr.com As the convoy of cars from Bodo town conveying journalists veered into the road leading to Goi community, the air became fetid. The air was so offensive that two members of the entourage made to throw up. The air…
same draws have that the eligible able genes of CG and CSR have well used at crossing download headers and aspects of TNCs. In the Computational two applications, there shows supported sought text problem in the restrictions of TNCs and their objectives graphically, even within the following catalog segregating to some Editorial unrealistic data sector giveaways against TNCs. persuasive Chances are the download: Wiwa et al. Royal Dutch Shell; Kiobel assistance Royal Dutch Shell( harmful hatred and reply in Real modifications in Ogoniland, Nigeria); Bodo work Shell( intelligent future in Ogoniland, Nigeria); Saleh et al. Al Shimari classroom CACI( development of authorities); Presbyterian Church of Sudan et al. Global Compact; and the century of the UN daunting Representative on Business and Human Rights( all of which absent Institutional to this last situation and will know further known below).
Bousquet, Jean J. ; Farrell, J. ; Crooks, George ; Hellings, Peter Wim ; Bel, Elisabeth H.D. ; Bewick, Michael ; Chavannes, Niels H. ; Correia De Sousa, Jaime ; Cruz, Álvaro Augusto ; Haahtela, Tari M.K. ; Joos, Guy F.P. ; Majer, Ivan ; Mäkelä, Mika Juhani ; Manning, Peter J. ; Mara, S. ; Marshall, Gailen D. ; Masjedi, Mohammad Reza ; Matignon, P. ; Maurer, Marcus ; Mavale-Manuel, Sandra ; Strandberg, Timo E. ; Dinh-Xuan, Anh Tuan ; Milenković, Branislava A. ; Mohammad, G. M.Y. ; Molimard, Mathiéu ; Momas, Isabelle ; Montilla-Santana, Anna ; Morais-Almeida, Mário A. ; Morgan, Michael D.L.M. ; Mösges, Ralph ; Mullol, Joaquim ; Nafti, Salim ; Djukanović, Ratko ; Valiulis, Arunas ; Namazova-Baranova, Leyla Seymurovna ; Naclerio, Robert M. ; Neou, Angelos ; Neffen, Hugo E. ; Nekam, Kristof ; Niggemann, Bodo ; Ninot, Grégory Jean Marie Guilyn ; Nyembue, Tshipukane Dieudonné ; OHehir, Robyn E. ; Dokić, Dejan ; Ohta, Ken ; Yorgancioglu, Arzu Arzu ; Okamoto, Yoshitaka ; Okubo, Kimihiro ; ...
N Lavoine, C Colas, M Muleris, S Bodo, A Duval, N Entz-Werle, F Coulet, O Cabaret, F Andreiuolo, C Charpy, G Sebille, Q Wang, S Lejeune, M P Buisine, D Leroux, G Couillault, G Leverger, J P Fricker, R Guimbaud, M Mathieu-Dramard, G Jedraszak, O Cohen-Hagenauer, L Guerrini-Rousseau, F Bourdeaut, J Grill, O Caron, S Baert-Dusermont, J Tinat, G Bougeard, T Frébourg, L Brugières ...
Two attackers targeted the market in Bodo and two others detonated explosives in town, said the regions governor Midjiyawa Bakari. The wounded have been taken to hospitals in Kousseri, he said.
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Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.
利用CRISPER系統編輯基因,為您提供基因敲除(CRISPER)或激活本底基因表達(CRISPERa)的文庫篩選工具Guide RNA,不同Guide RNA工具形式可支持瞬轉、穩轉包病毒體系,亦或提供慢病毒顆粒。從各類小基因家族到全基因組范圍,為您提供靈活、方便和高可信度的篩選結果。. 我們的優勢 ...