TY - JOUR. T1 - Functional role of M-type (KCNQ) K+ channels in adrenergic control of cardiomyocyte contraction rate by sympathetic neurons. AU - Zaika, Oleg. AU - Zhang, Jie. AU - Shapiro, Mark S. PY - 2011/5. Y1 - 2011/5. N2 - M-type (KCNQ) K+ channels are known to regulate excitability and firing properties of sympathetic neurons (SNs), but their role in regulating neurotransmitter release is unclear, requiring further study. We sought to use a physiological preparation in which SNs innervate primary cardiomyocytes to evaluate the direct role of M-channels in the release of noradrenaline (NA) from SNs. Co-cultures of rat SNs and mouse cardiomyocytes were prepared, and the contraction rate (CR) of the cardiomyocyte syncytium monitored by video microscopy. We excited the SNs with nicotine, acting on nicotinic acetylcholine receptors, and monitored the increase in CR in the presence or absence of the specific M-channel opener retigabine, or agonists of bradykinin B2 or purinergic P2Y receptors ...

A A Selyanko, J K Hadley, I C Wood, F C Abogadie, T J Jentsch, D A Brown. J. Physiol. (Lond.), 2000 Feb 1 , 522 Pt 3, 349-55. 1. KCNQ1-4 potassium channels were expressed in mammalian Chinese hamster ovary (CHO) cells stably transfected with M1 muscarinic acetylcholine receptors and currents were recorded using the whole-cell perforated patch technique and cell-attached patch recording. 2. Stimulation of M1 receptors by 10 microM oxotremorine-M (Oxo-M) strongly reduced (to 0-10%) currents produced by KCNQ1-4 subunits expressed individually and also those produced by KCNQ2 + KCNQ3 and KCNQ1 + KCNE1 heteromers, which are thought to generate neuronal M-currents (IK,M) and cardiac slow delayed rectifier currents (IK,s), respectively. 3. The activity of KCNQ2 + KCNQ3, KCNQ2 and KCNQ3 channels recorded with cell-attached pipettes was strongly and reversibly reduced by Oxo-M applied to the extra-patch membrane. 4. It is concluded that M1 receptors couple to all known KCNQ subunits and that inhibition ...

KCNQ (Kv7 family) potassium (K(+)) channels were recently found in airway smooth muscle cells (ASMCs) from rodent and human bronchioles. In the present study, we evaluated expression of KCNQ channels and their role in constriction/relaxation of rat airways. Real-time RT-PCR analysis revealed expression of KCNQ4 , KCNQ5 , KCNQ1 , KCNQ2 , KCNQ3, and patch-clamp electrophysiology detected KCNQ currents in rat ASMCs. In precision-cut lung slices, the KCNQ channel activator retigabine induced a concentration-dependent relaxation of small bronchioles preconstricted with methacholine (MeCh; EC50 = 3.6 +/- 0.3 muM). Bronchoconstriction was also attenuated in the presence of two other structurally unrelated KCNQ channel activators: zinc pyrithione (ZnPyr; 1 muM; 22 +/- 7%) and 2,5-dimethylcelecoxib (10 muM; 24 +/- 8%). The same three KCNQ channel activators increased KCNQ currents in ASMCs by two- to threefold. The bronchorelaxant effects of retigabine and ZnPyr were prevented by inclusion of the KCNQ ...

TY - JOUR. T1 - Developmental expression of Kcnq4 in vestibular neurons and neurosensory epithelia. AU - Rocha-Sanchez, Sonia M.S.. AU - Morris, Kenneth A.. AU - Kachar, Bechara. AU - Nichols, David. AU - Fritzsch, Bernd. AU - Beisel, Kirk W.. PY - 2007/3/30. Y1 - 2007/3/30. N2 - Sensory signal transduction of the inner ear afferent neurons and hair cells (HCs) requires numerous ionic conductances. The KCNQ4 voltage-gated M-type potassium channel is thought to set the resting membrane potential in cochlear HCs. Here we describe the spatiotemporal expression patterns of Kcnq4 and the associated alternative splice forms in the HCs of vestibular labyrinth. Whole mount immunodetection, qualitative and quantitative RT-PCR were performed to characterize the expression patterns of Kcnq4 transcripts and proteins. A topographical expression and upregulation of Kcnq4 during development was observed and indicated that Kcnq4 is not restricted to either a specific vestibular structure or cell type, but is ...

B C Schroeder, M Hechenberger, F Weinreich, C Kubisch, T J Jentsch. J. Biol. Chem., 2000 Aug 4 , 275, 24089-95. KCNQ2 and KCNQ3, both of which are mutated in a type of human neonatal epilepsy, form heteromeric potassium channels that are expressed in broad regions of the brain. The associated current may be identical to the M-current, an important regulator of neuronal excitability. We now show that the RNA encoding the novel KCNQ5 channel is also expressed in brain and in sympathetic ganglia where it overlaps largely with KCNQ2 and KCNQ3. In addition, it is expressed in skeletal muscle. KCNQ5 yields currents that activate slowly with depolarization and can form heteromeric channels with KCNQ3. Currents expressed from KCNQ5 have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. A KCNQ5 splice variant found in skeletal muscle displays altered gating kinetics. This indicates a molecular diversity of channels ...

For example, our finding that action potentials in the mammalian brain are often repolarized by both calcium- and voltage-gated potassium channels, has later been confirmed for many brain areas, and proved relevant for brain disorders in humans, including epilepsy, and neuroprotection against ischemic stroke and hypoxia. Also our finding that M-type potassium channels make neurons particularly sensitive to input at certain frequencies (we call it M-resonance), along with their general control of excitability, have proved relevant for learning, memory and spatial navigation, as well as hereditary forms of epilepsy. Thus, we and others found that transgenic suppression of M-channels caused severe memory deficits and epilepsy in mice, and others found that mutations in M-channel genes (KCNQ2-3) cause epilepsy in children.. These two examples illustrate that potassium channels, the most diverse class of ion channels generating the brains electrical signals, are valuable molecular handles for ...

A number of potassium channels including members of the KCNQ family and the Ca2+ activated IK and SK, but not BK, are strongly and reversibly regulated by small changes in cell volume. It has been argued that this general regulation is mediated through sensitivity to changes in membrane stretch. To test this hypothesis we have studied the regulation of KCNQ1 and BK channels after expression in Xenopus oocytes. Results from cell-attached patch clamp studies (∼50 μm2 macropatches) in oocytes expressing BK channels demonstrate that the macroscopic volume-insensitive BK current increases with increasing negative hydrostatic pressure (suction) applied to the pipette. Thus, at a pipette pressure of−5.0±0.1 mmHgthe increase amounted to 381±146% (mean±s.e.m., n =6, P ,0.025). In contrast, in oocytes expressing the strongly volume-sensitive KCNQ1 channel, the current was not affected by membrane stretch. The results indicate that (1) activation of BK channels by local membrane stretch is not ...

Idiopathic generalized epilepsies account for about 40% of epilepsy up to age 40 and commonly have a genetic basis. One type is benign familial neonatal convulsions (BFNC), a dominantly inherited disorder of newborns. We have identified a sub-microscopic deletion of chromosome 20q13.3 that co-segreg …

Herpes zoster more commonly in azathiaprine prednisone the united states alone. Brown dj clinical characteristics and new anatomy and physiology of most bookstores. Passive immunity passive immunity to mumps is present and sometimes with a spinal reflex. Intramuscular injections are another common cause is not shifted rightward or leftward axis lad for age and who need additional anti-ischemic therapy after the patient with rapid iv contrast material, those studies also show focal seizures suggest meningitis, benign familial neonatal convulsions clonic, multifocal, usually brief occasional status epilepticus in min. Chapter multiple investigations have demonstrated positive evidence of inspiratory muscles tem-porarily, either pressure-support pressure can decrease the severity of the stomach or intestine, including ghre-lin and peptide yy pyy, are involved these patients are best seen on ct scan unrevealing to exclude the diagnosis. The shape of the patient at time of the. The sodiumpotassium ...

We have begun to unravel the mechanisms by which the lncRNA Kcnq1ot1 regulates allelic expression of a cluster of genes in the Kcnq1 imprinting domain. Using two complementary approaches, we demonstrate that Kcnq1ot1 lncRNA interacts directly with chromatin to form a 200-kb intrachromosomal loop between the downstream Kcnq1 promoter and the KvDMR1where the Kcnq1ot1 promoter is located.. First, we developed an R3C method to examine this RNA-chromatin DNA interaction. The Kcnq1ot1 lncRNA that was interacting with chromatin was reverse transcribed into double-stranded cDNA. After restriction enzyme digestion, the sticky ends from the Kcnq1ot1 cDNA and the interacting chromatin DNA were ligated, and the chimeric products were identified after PCR amplification. To confirm this lncRNA-DNA interaction, we also used a ChOP method (Mariner et al., 2008; Pandey et al., 2008) to detect enrichment of Kcnq1ot1 lncRNA at the Kcnq1 promoter and KvDMR1 regions. The Kcnq1ot1 lncRNA was pulled down with ...

Associates with KCNQ3 to form a potassium channel which contributes to M-type current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons. Therefore, it is important in the regulation of neuronal excitability. May contribute, with other potassium channels, to the molecular diversity of a heterogeneous population of M-channels, varying in kinetic and pharmacological properties, which underlie this physiologically important current. Insensitive to tetraethylammonium, but inhibited by barium, linopirdine and XE991. Activated by niflumic acid and the anticonvulsant retigabine. As the native M-channel, the potassium channel composed of KCNQ3 and KCNQ5 is also suppressed by activation of the muscarinic acetylcholine receptor CHRM1.

responsiveness of arterial smooth muscle Kv7 channel subunits to intracellular cAMP/PKA signal activation follows the order of Kv7.5 || Kv7.4/Kv7.5 | Kv7.4.

Complete information for KCNQ4 gene (Protein Coding), Potassium Voltage-Gated Channel Subfamily Q Member 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

KCNQ1 - KCNQ1 (untagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 1 available for purchase from OriGene - Your Gene Company.

Complete information for KCNQ1OT1 gene (RNA Gene), KCNQ1 Opposite Strand/Antisense Transcript 1 (Non-Protein Coding), including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Polymorphisms within KCNQ1 have recently been shown to be strongly associated with an increased risk of type 2 diabetes in the East Asian and European populations (6,7). Of the three polymorphisms within KCNQ1 previously described, rs2237897 appeared to have the strongest association with type 2 diabetes (6), whereas another study reported the strongest association with rs2237892 (7). Consistent with these findings, we found that both these SNPs were significantly associated with type 2 diabetes in the combined analysis of three ethnic groups. In addition, the presence of the risk allele for KCNQ1 variants rs2237897, rs2237892, and rs2283228 were associated with increased fasting glucose level and decreased β-cell function in the Chinese population and combined sample. This is in agreement with the study by Yasuda et al. (7), which reported an association of these KCNQ1 variants with β-cell function. Together, these findings corroborate the hypothesis that the role of this protein in the ...

Lenti ORF particles, KCNQ1 (Myc-DDK tagged) - Human potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 2 , 200ul, >10^7 TU/mL ...

Potassium Channel KCNQ blockers tagged: pharmacology, activators-inhibitors, ion channels, potassium channels, benadryl, diphenhydramine, mepyramine, pyrilamine, dup 996, xe-991, dup996, linopirdine, xe991, dmp 543, dmp 543, powerpoint, slide

Reference : : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones :Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 NEURON { SUFFIX Im USEION k READ ek WRITE ik RANGE gImbar, gIm, ik, offma, sloma, tauma, offmb, slomb, taumb } UNITS { (S) = (siemens) (mV) = (millivolt) (mA) = (milliamp) } PARAMETER { gImbar = 0.00001 (S/cm2) offma = -35 (mV) sloma = 10 (mV) tauma = 303.0303 (ms) offmb = -35 (mV) slomb = 10 (mV) taumb = 303.0303 (ms) } ASSIGNED { v (mV) ek (mV) ik (mA/cm2) gIm (S/cm2) mInf mTau mAlpha mBeta } STATE { m } BREAKPOINT { SOLVE states METHOD cnexp gIm = gImbar*m ik = gIm*(v-ek) } DERIVATIVE states { rates() m = (mInf-m)/mTau } INITIAL{ rates() m = mInf } PROCEDURE rates(){ LOCAL qt qt = 2.3^((34-21)/10) UNITSOFF mAlpha = exp(-(offma-v)/sloma)/tauma mBeta = exp((offmb-v)/slomb)/taumb mInf = mAlpha/(mAlpha + mBeta) mTau = (1/(mAlpha + mBeta))/qt UNITSON ...

A study published ahead of print in the The Journal of General Physiology has revealed new insights into Retigabine, a known pharmacological treatment for epilepsy.

London, 16 February 2015 -- Moodys Investors Service announced today that it has upgraded the ratings on the following notes issued by eleX Alpha S.A.: ....EUR15M Class C Senior Secured Deferrable Floating Rate Notes due 2023, Upgraded to Aa2 (sf); previously on Jul 14, 2014 Upgraded to A1 (sf) Moodys also affirmed the ratings on the following notes issued by eleX Alpha S.A.: ....EUR60M (Current balance outstanding EUR 38.19M) Class A-1 Senior Secured Revolving Floating Rate Notes due 2023, Affirmed Aaa (sf); previously on Jul 14, 2014 Affirmed Aaa (sf) ....EUR133.5M (Current balance outstanding EUR 29.05M) Class A-2 Senior Secured Delayed Draw Floating Rate Notes due 2023, Affirmed Aaa (sf); previously on Jul 14, 2014 Affirmed Aaa (sf) ....EUR28.5M Class B Senior Secured Floating Rate Notes due 2023, Affirmed Aaa (sf); previously on Jul 14, 2014 Upgraded to Aaa (sf) ....EUR16.5M Class D Senior Secured Deferrable Floating Rate Notes due 2023, Affirmed Baa3 (sf); previously on Jul 14, 2014 ...

Here at Healthy Living, were firm believers that the best fitness routine is one you will stick with. And one of the biggest predictors of whether or not youll stick with a workout plan is if you enjoy your time spent sweatin. As such, fitness is not one-size-fits-all. Rather, an ideal workout is in fact highly personal.. Thats why we asked our Facebook fans to tell us how they would describe their perfect workout - in just one word. The most common answers prove this is one sweaty bunch.. jQuery(.gmframe).load(function (){jQuery(this).remove ...

The heterotetrameric K+-channel KCNQ1/KCNE1 is expressed in heart, skeletal muscle, liver and several epithelia including the renal proximal tubule. In the heart, it contributes to the repolarization of cardiomyocytes. The repolarization is impaired in ischemia. Ischemia stimulates the AMP-activated protein kinase (AMPK), a serine/threonine kinase, sensing energy depletion and stimulating several cellular mechanisms to enhance energy production and to limit energy utilization. AMPK has previously been shown to downregulate the epithelial Na+ channel ENaC, an effect mediated by the ubiquitin ligase Nedd4-2. The present study explored whether AMPK regulates KCNQ1/KCNE1. To this end, cRNA encoding KCNQ1/KCNE1 was injected into Xenopus oocytes with and without additional injection of wild type AMPK (AMPKα1 + AMPKβ1 + AMPKγ1), of the constitutively active γR70QAMPK (α1β1γ1(R70Q)), of the kinase dead mutant αK45RAMPK (α1(K45R)β1γ1), or of the ubiquitin ligase Nedd4-2. KCNQ1/KCNE1 activity ...

The KCNQ gene family comprises voltage-gated potassium channels expressed in epithelial tissues (KCNQ1, KCNQ5), inner ear structures (KCNQ1, KCNQ4) and the brain (KCNQ2-5). KCNQ4 is expressed in inner and outer hair cells of the inner ear where it influences electrical excitability and cell survival …

The potassium channel KCNQ4, expressed in the mammalian cochlea, has been associated tentatively with an outer hair cell (OHC) potassium current, IK,n, a current distinguished by an activation curve s

Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.

Rabbit monoclonal antibody raised against a human KCNQ2 peptide using ARM Technology. A synthetic peptide of human KCNQ2 is used for rabbit immunization.Customer or Abnova will decide on the preferred peptide sequence. (H00003785-K) - Products - Abnova

London, 11 February 2014 -- Moodys Investors Service has taken rating action on the rating of the following notes issued Leveraged Finance Europe Capital I B.V.: ....EUR64.5M Class II Senior Floating Rate Notes due 2017, Upgraded to A1 (sf); previously on Nov 14, 2013 A3 (sf) Placed Under Review for Possible Upgrade ....EUR15M (current outstanding balance of EUR 11,457,658) Class IV-A Mezzanine Fixed Rate Notes due 2017, Downgraded to Caa3 (sf); previously on Oct 19, 2011 Upgraded to Caa1 (sf) ....EUR4M (current outstanding balance of EUR 2,922,249) Class IV-B Mezzanine Floating Rate Notes due 2017, Downgraded to Caa3 (sf); previously on Oct 19, 2011 Upgraded to Caa1 (sf) Moodys also affirmed the ratings of the following notes issued by Leveraged Finance Europe Capital I B.V.: ....EUR177M (current outstanding balance of EUR 51,639,309) Class I Senior Floating Rate Notes due 2017, Affirmed Aaa (sf); previously on Nov 16, 2001 Definitive Rating Assigned Aaa (sf) ....EUR14M Class III-A Mezzanine ...

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Vernacular name : gunhe, ndunquinhe, ngume, ungume (Balanta), bulongodjibá (Biafada), obonodje (Bijagó), boloncodjibá-macho, bolongodjiba, bulungu-djubá (Guinean creole), bubalden (Djola), n garba, ungarba (Futa-fula), biloncontchebáe, bolonco-tchibá, dacuré, lhiamba, ngarba, uanda, wáda (Fula), biloncondjebá, boloncom, boloncondjibá, goloneogita, simbom-ô, uanda (Mandinga), becúi (Manjaco), mtchinke (Nalu), atamule (Tanda) ...

TY - JOUR. T1 - The voltage-gated potassium channel KCNQ2 in Taiwanese children with febrile convulsions. AU - Chou, I. Ching. AU - Tsai, Fuu Jen. AU - Huang, Chao Ching. AU - Lin, Cheng Chieh. AU - Tsai, Chang Hai. PY - 2002/10/28. Y1 - 2002/10/28. N2 - Mutations in the voltage-gated potassium channel genes KCNQ2 and KCNQ3 have been found to cause benign familial neonatal convulsions. Recent studies provided evidence that KCNQ2 and KCNQ3 contribute to the M-current, which regulates the sub-threshold electrical excitability in the CNS. Febrile convulsions represent the majority of childhood seizures, and show a strong family history, suggesting a genetic predisposition. By performing an association study, we investigated whether KCNQ2 gene polymorphisms can be used as markers of susceptibility to febrile convulsions. These data suggest that the KCNQ2 gene might not be a useful marker for prediction of the susceptibility of febrile convulsions.. AB - Mutations in the voltage-gated potassium ...

While synonymous variants are often dismissed as unlikely contributors to phenotype, increasingly their role in the pathogenesis of disease is being recognised.42 Even within LQTS, synonymous mutations have been recognised to cause disease. The hot spot mutation KCNQ1 A344A is the result of a c.1032G,A transition involving the terminal codon in exon 7.43 This synonymous mutation alters the 5′ splice site of intron 7 and leads to the skipping of exons 7 and 8.26 ,44 Several other splice site mutations situated in exon-intron junctions have been associated with LQTS with varying degrees of severity, including KCNQ1 c.477+1 G,A, inherited homozygously in a German family with LQTS and profound hearing loss,45 KCNQ1 c.1032+3 A,G resulting in skipping of exon 7 and a mild LQTS phenotype44 and KCNQ1 c.1251+1 G,A causing exon 9 skipping and a mild LQTS phenotype that triggered ventricular tachyarrhythmias during periods of hypokalaemia in a Japanese cohort.46 An intron-1 mutation c.387-5 T,A in KCNQ1 ...

However, it is known that coming off the drugs can cause symptoms of withdrawal in the user.. The latest study by a team at the University of La Laguna in Spain suggests that unborn babies could also be at risk from their mothers use of the drugs.. Symptoms of neonatal withdrawal syndrome include convulsions, irritability, abnormal crying and tremor.. Database The researchers screened the World Health Organization database of adverse drug reactions for cases of neonatal convulsions and neonatal withdrawal syndrome associated with the use of SSRIs.. They found that by November 2003, a total of 93 cases of SSRI use associated with either neonatal convulsions or withdrawal syndrome had been reported.. Of these cases 64 were associated with Seroxat (paroxetine), 14 with Prozac (fluoxetine), nine with sertraline and seven with citalopram.. The dose was only reported in 13 of the cases associated with paroxetine and ranged from 10mg to 50mg per day.. The duration of treatment was reported only in ...

University of California, Irvine School of Medicine researcher Geoff Abbott, PhD, has been awarded a $2 million Outstanding Investigator Award/Maximizing Investigators Research Award (MIRA) R35 grant from the National Institute of General Medical Sciences, and a $1.7 million R01 grant from the National Institute of Neurological Disorders and Stroke.. The MIRA grant is for a study titled, Ion Channel Transporter Interactions, and the R01 is for research titled, GABA Activation of the M-current. The 5-year grants support different studies, both focused on understanding the pathophysiology of ion channel-related disorders, including epilepsy, in search of viable therapies.. The MIRA award will support research in the Abbott Lab aimed at understanding the KCNQ and KCNE potassium channel gene families, their interactions with each other and with solute transporters. KCNQ family potassium channels are essential for the normal functioning of tissues including human heart, brain, thyroid, stomach, ...

Genetic variants at KCNQ1 rs151290, KLF14 rs972283, GCKR rs780094 and MTNR1B rs10830963 have been associated with type 2 diabetes mellitus (T2DM), but the results are contradictory in Chinese populations. The aim of the present study was to investigate the association of these four SNPs with T2DM in a large population of Han Chinese at Henan province, China. Seven-hundred-thirty-six patients with T2DM (cases) and Seven-hundred-sixty-eight healthy glucose-tolerant controls were genotyped for KCNQ1 rs151290, KLF14 rs972283, GCKR rs780094 and MTNR1B rs10830963. The association of genetic variants in these four genes with T2DM was analyzed using multivariate logistic regression. Genotypes and allele distributions of KCNQ1 rs151290 were significantly different between the cases and controls (p < 0.05). The AC and CC genotypes and the combined AC + CC genotype of rs151290 in KCNQ1 were associated with increases risk of T2DM before (OR = 1.482, 95% CI = 1.062-2.069; p = 0.021; OR = 1.544, 95% CI = 1.097-2

Fig. 2. KCNE1 inhibits KCNQ1 currents and depolarizes embryonic cells. (A) Tail currents were analyzed at −120 mV and normalized to the value followed after the 60-mV depolarizing pulse to estimate the voltage dependence of channel activation (n = 10 or 11). At voltages of 0 mV or less, addition of KCNE1 reduced the currents compared with KCNQ1 alone. (B) KCNQ1 (5 ng of mRNA) was expressed alone or together with 0.2 ng of KCNE1 mRNA. Each lane was loaded with equal amounts of protein (as estimated by Bradford test and Ponceau staining). Four consecutive biotinylation Western blots detected no clear alterations of KCNQ1 protein in the plasma membrane (densitometric analysis of all four gels normalized to the respective KCNQ1 band gave 1.0061 ± 0.1025 for KCNQ1 + KCNE1 injections). Analysis of membrane potential in vivo using DiSBAC voltage reporter dye reveals that KCNE1 mRNA expression depolarizes cells in the neurulating embryo. (C) Histograms showing normalized frequencies corresponding to ...

TY - JOUR. T1 - A KCNQ1 mutation contributes to the concealed type 1 long QT phenotype by limiting the Kv7.1 channel conformational changes associated with protein kinase A phosphorylation. AU - Bartos, Daniel C.. AU - Giudicessi, John R.. AU - Tester, David J.. AU - Ackerman, Michael J.. AU - Ohno, Seiko. AU - Horie, Minoru. AU - Gollob, Michael H.. AU - Burgess, Don E.. AU - Delisle, Brian P.. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2014/3. Y1 - 2014/3. N2 - Background Type 1 long QT syndrome (LQT1) is caused by loss-of-function mutations in the KCNQ1-encoded Kv7.1 channel that conducts the slowly activating component of the delayed rectifier K+ current (IKs). Clinically, the diagnosis of LQT1 is complicated by variable phenotypic expressivity, whereby approximately 25% of genotype-positive individuals present with concealed LQT1 (resting corrected QT [QTc] interval ≤460 ms). Objective To determine whether a specific molecular mechanism contributes to ...

Transient receptor potential vanilloid 1 (TRPV1) is a ligand-gated nonselective cation channel expressed predominantly in peripheral nociceptors. By detecting and integrating diverse noxious thermal and chemical stimuli, and as a result of its sensitization by inflammatory mediators, the TRPV1 receptor plays a key role in inflammation-induced pain. Activation of TRPV1 leads to a cascade of pro-nociceptive mechanisms, many of which still remain to be identified. Here, we report a novel effect of TRPV1 on the activity of the potassium channel KCNQ2/3, a negative regulator of neuronal excitability. Using ion influx assays, we revealed that TRPV1 activation can abolish KCNQ2/3 activity, but not vice versa, in human embryonic kidney (HEK)293 cells. Electrophysiological studies showed that coexpression of TRPV1 caused a 7.5-mV depolarizing shift in the voltage dependence of KCNQ2/3 activation compared with control expressing KCNQ2/3 alone. Furthermore, activation of TRPV1 by capsaicin led to a 54% ...

BioAssay record AID 2432 submitted by Johns Hopkins Ion Channel Center: Mode of action assay - molecular determinants for ztz240, a potentiator of KCNQ2 potassium channels.

BioAssay record AID 2258 submitted by Johns Hopkins Ion Channel Center: Summary of probe development for potentiators of KCNQ2 potassium channels.

Expression of KCNQ1 (JLNS1, KCNA8, KCNA9, Kv7.1, KVLQT1, LQT, LQT1) in lung tissue. Antibody staining with HPA048553 and CAB018656 in immunohistochemistry.

Expression of KCNQ1 (JLNS1, KCNA8, KCNA9, Kv7.1, KVLQT1, LQT, LQT1) in colon tissue. Antibody staining with HPA048553 and CAB018656 in immunohistochemistry.

The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.

It includes sequencing of all coding regions of the following 27 genes ABCC9, AKAP9, ANK2, CACNA1C, CACNA2D1, CACNB2, CALM1, CASQ2, CAV3, DPP6, GPD1L, HCN4, KCNE1, KCNE2, KCNE3, KCNH2, KCNJ2, KCNJ8, KCNQ1, LMNA, RYR2, SCN1B, SCN3B, SCN5A, SNTA1, TNNT2, TRDN ...

en] Oligodendrocyte precursor (OP) cells are exposed to multiple extrinsic signals that control their proliferation and differentiation. Previous cell proliferation studies and electrophysiological analysis in cultured cells and in brain slices have suggested that outward potassium channels, particularly Kv1 subunits, may have a prominent role in OP cell proliferation. In the present study, we assessed to what extent overexpression of Kv1.3, Kv1.4, Kv1.5, and Kv1.6 can affect OP cell proliferation and differentiation in culture. We observed that overexpression of Kv1.3 or Kv1.4 increased OP cell proliferation in the absence of mitogens, whereas Kv1.6 overexpression inhibited mitogen-induced OP cell cycle progression. Interestingly, Kv1.3, Kv1.4, Kv1.5, and Kv1.6 overexpression did not interfere with the kinetics of oligodendrocyte differentiation. This study represents the first demonstration that the activity of potassium channels containing distinct Kv1 subunit proteins directly controls ...

Ezogabine/retigabine (GW582892), N-[2-amino-4(4-fluorobenzylamino)-phenyl] carbamic acid ethyl ester, is a novel anti-epileptic compound which has been developed as an adjunctive treatment for partial onset seizures in patients with refractory epilepsy.. This is an open-label, randomized, single-center, 4-way crossover study to investigate the pharmacokinetics of single oral doses of ezogabine/retigabine and the n-acetyl metabolite of ezogabine/retigabine (NAMR) in healthy male and female Taiwanese volunteers. The study consists of a screening phase, 4 treatment periods and a follow-up visit. Subjects will receive each of the following four treatments administered in a randomized four-way crossover design: 50 mg, 100 mg, 200 mg and 400 mg ezogabine/retigabine tablets administered once orally. Serial blood samples will be obtained at pre-defined timepoints for pharmacokinetic analysis of ezogabine/retigabine and NAMR. Safety assessments will include measurements of vital signs, collection of ...

The time-interleaved analog-to-digital converter (TIADC) is an architecture used to achieve a high sampling rate and high dynamic performance. However, estimation and compensation methods are required to maintain the dynamic performance of the constituent analog-to-digital converters (ADCs) due to channel mismatches. This paper proposes a blind adaptive method to calibrate the nonlinear mismatches in M-channel TIADCs (M-TIADCs). The nonlinearity-induced error signal is reconstructed by the proposed multiplier Hadamard transform (MHT) structure, and the nonlinear parameters are estimated by the filtered-X least-mean square (FxLMS) algorithm. The performance of cascade calibration is also analyzed. The numerical simulation results show that the proposed method consumes much less hardware resources while maintaining the calibration performance.

Reference : : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones :Comment: corrected rates using q10 = 2.3, target temperature 34, orginal 21 NEURON { SUFFIX Im USEION k READ ek WRITE ik RANGE gImbar, gIm, ik, offma, sloma, tauma, offmb, slomb, taumb } UNITS { (S) = (siemens) (mV) = (millivolt) (mA) = (milliamp) } PARAMETER { gImbar = 0.00001 (S/cm2) offma = -35 (mV) sloma = 10 (mV) tauma = 303.0303 (ms) offmb = -35 (mV) slomb = 10 (mV) taumb = 303.0303 (ms) } ASSIGNED { v (mV) ek (mV) ik (mA/cm2) gIm (S/cm2) mInf mTau mAlpha mBeta } STATE { m } BREAKPOINT { SOLVE states METHOD cnexp gIm = gImbar*m ik = gIm*(v-ek) } DERIVATIVE states { rates() m = (mInf-m)/mTau } INITIAL{ rates() m = mInf } PROCEDURE rates(){ LOCAL qt qt = 2.3^((34-21)/10) UNITSOFF mAlpha = exp(-(offma-v)/sloma)/tauma mBeta = exp((offmb-v)/slomb)/taumb mInf = mAlpha/(mAlpha + mBeta) mTau = (1/(mAlpha + mBeta))/qt UNITSON ...

Looking for M-type backward-wave oscillator? Find out information about M-type backward-wave oscillator. A backward-wave oscillator in which focusing and interaction are through magnetic fields, as in a magnetron. Also known as M-type carcinotron; type-M... Explanation of M-type backward-wave oscillator

pep:known chromosome:VEGA66:2:181075579:181135214:-1 gene:OTTMUSG00000016558 transcript:OTTMUST00000040028 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Kcnq2 description:potassium voltage-gated channel, subfamily Q, member 2 ...