We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing |TEX|$\beta$|/TEX|-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 |TEX|$\mu$|/TEX|M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment

In ,i,Vanda,/i, orchids, it is important to produce cultivars with economically important traits such as disease and pest resistances and novel flower colors, which are difficult to achieve by conventional cross breeding methods. To realize these breeding objectives, it is now expected to apply genetic transformation technology to introduce useful foreign genes into ,i,Vanda,/i, orchids. However, there has been almost no information on the genetic transformation of ,i,Vanda,/i,. Transgenic plants were successfully regenerated after co-cultivating protocorm-like bodies (PLBs) with ,i,Agrobacterium tumefaciens,/i, strain EHA101 (pIG121Hm) that harbored genes for β-glucuronidase (,i,gus,/i,), hygromycin phosphotransferase (,i,hpt,/i,) and neomycin phosphotransferase II (,i,nptII,/i,). PLBs of Tokyo Blue maintained in liquid New Dogashima medium (NDM) under dark condition, were subjected to transformation experiments. The PLBs inoculated with ,i,Agrobacterium,/i, produced secondary PLBs 4 weeks ...

The rose OS10033701 flowers have been modified for mauve colouration through the biosynthesis and accumulation of the anthocyanin pigment delphinidin, which is not produced in non-transgenic roses. To achieve the unique coloration, viola flavonoid 3, 5-hydroxylase (F35H) and iris dihydroflavonol reductase (DFR) were inserted to produce blue-coloured delphinidin anthocyanin pigments. F35H directs the production of an intermediate dihydromyricetin, which is then utilized as a substrate by iris DFR to produce delphinidin. A third gene gene cassette silences the expression of the endogenous rose DRF, which is responsible for the production of cyanidin, through an RNA interference response to prevent cyanidin production. Thus, the intermediate metabolites are used for delphinidin biosynthesis instead. The rose additionally contains a selectable marker (kanamycin resistance), E. coli neomycin phosphotransferase II, for the selection of transformants during cloning ...

Mutant strains containing APH(3)-II were constructed via site-directed mutagenesis of the cloned gene and by random mutagenesis of a strain containing the APH(3)-II gene on a conjugative plasmid. Substitutions at highly conserved amino acid residues produced APH(3) enzymes which in general showed reduced activity and conferred reduced levels of resistance to their substrates. Substitutions at Tyr 218 altered substrate specificity for the enzymes. Random mutagenesis produced plasmid-borne mutations conferring amikacin resistance. Two of these mutations appeared to be localized to the APH(3)-II structural gene ...

The aminoglycoside phosphotransferases (APHs) are responsible for the bacterial inactivation of many clinically useful aminoglycoside antibiotics. We report the characterization of an enterococcal enzyme, APH(3)-IIIa, which inactivates a broad spectrum of aminoglycosides by ATP-dependent O-phosphorylation. Overproduction of APH(3)-IIIa has permitted the isolation of 30-40 mg of pure protein/(L of cell culture). Purified APH(3)-IIIa is a mixture of monomer and dimer which is slowly converted to dimer only over time. Dimer could be dissociated into monomer by incubation with 2-mercaptoethanol, suggesting that dimerization is mediated by formation of disulfide bond(s). Both monomer and dimer show Km values in the low micromolar range for good substrates such as kanamycin and neomycin, and kcat values of 1-4 s-1. All aminoglycosides show substrate inhibition except amikacin and kanamycin B. Determination of minimum inhibitory concentrations indicates a positive correlation between antibiotic activity and

The Sp2/mIL-6 line was established by R. Hawley and T. Hawley of the Sunnybrook Health Centre, Toronto, Canada. It is a mouse fusion cell line derived from Sp2/0 cells that had been transfected with high-titer L6PNL recombinant retrovirus carrying a mouse interleukin-6 (IL-6) cDNA under the transcriptional control of the viral LTR and the neo gene encoding bacterial neomycin phosphotransferase (G418 resistance).

The Sp2/mIL-6 line was established by R. Hawley and T. Hawley of the Sunnybrook Health Centre, Toronto, Canada. It is a mouse fusion cell line derived from Sp2/0 cells that had been transfected with high-titer L6PNL recombinant retrovirus carrying a mouse interleukin-6 (IL-6) cDNA under the transcriptional control of the viral LTR and the neo gene encoding bacterial neomycin phosphotransferase (G418 resistance). 

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Summary The polymerase chain reaction (PCR) was used to identify the aacA-aphD, aphA3 and aadC genes, encoding the aminoglycoside-modifying enzymes AAC(6′)-APH(2′), APH(3′)III and ANT(4′ 4

Halls PhD was on the enzymology of B-lactamase, which led to his first paper being published in Nature in 1976. He used strains of E. Coli with mutated B-lactamase, an antibiotic resistance enzyme, and assayed their activity in the presence of Benzylpenicillin and Cephalosporin C. Direct selection on these mutants allowed catalytic properties of B-lactamase to be identified and allowed structure-function relationships of the enzyme to be further researched.[4] In 1981 he went to work at Institute for Cancer Research in London, where he stayed for 12 years. His work, in collaboration with his colleague and close friend Christopher Marshall, made seminal contributions to our understanding of cell signalling in animal cells, in particular the role of Rho and Ras small GTPases in regulating a variety of cellular functions such as proliferation, morphology and migration. In 1982, Hall helped identify transforming sequences in human sarcoma cells lines at the Institute for Cancer Research in London. ...

ID PTACNEO12 preliminary; circular DNA; SYN; 5440 BP. XX AC ATCC37687; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Vertebrate/E.coli plasmid vector pTacNeo1.2 - incomplete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pTacNeo1.2 from pBR322 & tac promoter & Tn601/Tn5 APH(3)-I gene RA Hunter-Cevera J.C.; RT ; RL Unpublished (1993). XX CC The upstream translation of LacZ is out of frame with APH(3)-I. CC (personal communication) CC The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 CC amino-terminal codons of beta-galactosidase, & a gene encoding a CC modified aminoglycoside phosphotransferase (3)-I (APH(3)-I). CC (personal communication) CC The start of the modified APH(3)-I is followed by the eleventh codon CC (serine) of APH(3)-I. Codons 2-10 have been deleted. (personal CC communication) CC Sequences from the TAA stop codon of APH(3)-I to the immediate CC downstream ...

Catalytic domain of the dual-specificity Protein Kinases, MAP kinase kinases 3 and 6. Protein kinases (PKs), MAP kinase kinase 3 (MKK3) and MKK6 subfamily, catalytic (c) domain. PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. The MKK3 and MKK6 subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. The mitogen-activated protein (MAP) kinase signaling pathways are important mediators of cellular responses to extracellular signals. The pathways involve a triple kinase core cascade comprising the MAP kinase (MAPK), which is phosphorylated and activated by a MAPK kinase (MAPKK or MKK), which itself is phosphorylated and activated by a MAPK kinase kinase (MAPKKK or MKKK). MKK3 and MKK6 are dual-specificity PKs that phosphorylate and ...

Catalytic domain of the dual-specificity Protein Kinase, MAP kinase kinase 7. Protein kinases (PKs), MAP kinase kinase 7 (MKK7) subfamily, catalytic (c) domain. PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. The MKK7 subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. The mitogen-activated protein (MAP) kinase signaling pathways are important mediators of cellular responses to extracellular signals. The pathways involve a triple kinase core cascade comprising the MAP kinase (MAPK), which is phosphorylated and activated by a MAPK kinase (MAPKK or MKK), which itself is phosphorylated and activated by a MAPK kinase kinase (MAPKKK or MKKK). MKK7 is a dual-specificity PK that phosphorylates and activates its downstream target, c-Jun ...

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.. About ASM , Contact Us , Press Room. ASM is a member of. ...

tRNA 2-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1-2cyclic phosphate and ...

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Neo is a medicine available in a number of countries worldwide. A list of US medications equivalent to Neo is available on the Drugs.com website.

ACC Neo is a medicine available in a number of countries worldwide. A list of US medications equivalent to ACC Neo is available on the Drugs.com website.

Hi, I have a Thinkware U1000, and a Cellink Neo. My problem is that I have only the following cable for the Thinkware dashcam, what connects to fusebox...

This research describes the optimization of parameters (including pH, temperature, period of co-cultivation and age of callus) for Agrobacterium tumefaciens-mediated genetic transformation of Theobroma cacao L. using staminodes from cocoa buds as explants. The A. tumefaciens strain used was the super avirulent AGLl with the binary vector pGPTV-Kan/Gus. The strain confers aminoglycoside resistance to transformed cells through the neomycin phosphotransferase II (nptII) gene. Callus induction medium contained DKW minerals, glucose, vitamins, 2 mg/L 2,4D and 0.005 mg/L TDZ (0.5nM) pH 5.3. Co-cultivation medium was identical to callus induction medium but contained 0.02mg/L acetosyringone. Experiments were conducted using two clones of cocoa: KKM19 and P22. Staminodes were cultured on callus induction medium in the dark before the transformation process. After 14 days and 21 days on callus induction medium, callus-derived staminodes were co-cultivated with A. tumefaciens on semi-solid co-cultivation ...

The three classes of enzymes which inactivate aminoglycosides and lead to bacterial resistance are reviewed. DNA hybridization studies have shown that different genes can encode aminoglycoside-modifying enzymes with identical resistance profiles. Comparisons of the amino acid sequences of 49 aminoglycoside-modifying enzymes have revealed new insights into the evolution and relatedness of these proteins. A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from these data and from the results of mutational analysis of several of the genes encoding aminoglycoside-modifying enzymes. Recent studies have demonstrated that aminoglycoside resistance can emerge as a result of alterations in the regulation of normally quiescent cellular genes or as a result of acquiring genes which may have originated from aminoglycoside-producing organisms or from other resistant organisms. Dissemination of these genes is aided by a variety of genetic elements ...

|p||strong|Technical Advantage:|/strong|  Effective against both Gram-positive and -negative bacteria|br /| Kanamycin is a member of the aminoglycosides a group of molecules and interacts with the 30S subunit of prokaryotic ribosomes.  It induces errors in translation and thus hinders protein synthesis.  The KanR-Tn5 gene product (aminoglycoside phosphotransferase) confers resistance to kanamycin.  Kanamycin Sulfate is effective against Gram-negative and -positive bacteria.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| -  Produced under the highest industry standards to ensure superior results|/p|

APH1A - APH1A (untagged)-Human anterior pharynx defective 1 homolog A (C. elegans) (APH1A), transcript variant 2 available for purchase from OriGene - Your Gene Company.

Eered a targeting construct that firstly, included the introduction of a floxed 2.1 kb, Neomycin resistance (Neor) cassette under the control of the

Study Flashcards On aph final at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!

Plasmid pLenti CMVTRE3G Neo DEST (w813-1) from Dr. Eric Campeaus lab contains the insert Empty. This plasmid is available through Addgene.

In article ,Pine.SOL.3.96.970711115134.16570A-100000 at ascc.artsci.wustl.edu,, Alex Brands ,abbrands at artsci.wustl.edu, wrote: , I was planning to use kanamycin resistance as a selectable marker in , yeast, and I aquired a construct from another lab that has a kanamycin , resistance cassette. However, my negative control plates revealed that , the parent yeast (w303) was not sensitive to the kanamycin. After talking , to the other lab, I found out that they use something called Geneticin, , (that name is a registered trademark of GIBCO) which is about 20 times , more expensive than kanamycin. , , So, is my kanamycin simply expired, or are yeast not sensitive to , kanamycin? Am I stuck with Geneticin? , , , All help is appreciated very much! , , Alex Brands , Washington University Sorry, youre stuck with the Geneticin (also known as G418). Although the kanamycin resistance factor inactivates both kanamycin and G418, only the latter antibiotic is effective against eukaryotic cells. Steve ...

lactamases are the most widespread resistance mechanism to -lactams and are categorized into four classes, each exhibiting a unique mechanism for destruction of -lactam substrates. Of particular concern are the class D -lactamases, which hydrolyze several of the most potent -lactams in clinical use. In part, resistance derives from the structural similarity of the inhibitors to the -lactams themselves. Therefore an urgent need exists for novel inhibitors that do not resemble -lactams. Research in the Powers lab employs a structure-based approach to identify and characterize binding sites on the class D -lactamases OXA-1 and OXA-24 and will be used to discover novel, non- -lactam inhibitors for these key resistance enzymes using molecular docking.. ...

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Hygromycin B solution is a selection antibiotic for mammalian and bacterial cells. It is sterile, cell culture tested, and available as a solution. CAS 31282-04-9

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Background & objective: Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to aminoglycosides. The aim of this study was to determine the prevalence of 16S rRNA methylase (armA, rmtA, rmtB, rmtC, and rmtD), and the AME genes [aac(6′)-Ib, aac(3)-I, ant(3′′)-I, aph(3′)-I and aac(6)-Id], among clinical isolates of A. baumannii in Tehran, Iran. Methods: Between November 2015 to July 2016, a total of 110 clinical strains of A. baumannii were isolated from patients in two teaching hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute guidelines. The presence of genes encoding the AMEs and16S rRNA methylases responsible for resis-tance was investigated by

Shop a large selection of Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL) products and learn more about Gibco™ Geneticin™

Aph1a - Lenti ORF clone of Aph1a (Myc-DDK-tagged ORF) - Rat anterior pharynx defective 1 homolog A (C. elegans) (Aph1a), (10 ug) available for purchase from OriGene - Your Gene Company.

Learn more about Kanamycin Sulfate, OmniPur*.. We enable science by offering product choice, services, process excellence and our people make it happen.

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Most of the combined products containing more than one antibiotic, contain neomycin. Neomycin is given classification priority, thus all combined products containing neomycin and other antibiotics should be classified in A07AA51 - neomycin, combinations ...

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0159] Resistance genes for glyphosate (resistance conferred by mutant 5-enolpyruvl-3 phosphikimate synthase (EPSP) and aroA genes, respectively), and hygromycin B phosphotransferase, and to other phosphono compounds such as glufosinate (phosphinothricin acetyl transferase (PAT) and Streptomyces hygroscopicus phosphinothricin-acetyl transferase (bar) genes) may also be used. See, for example, U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotide sequence of a form of EPSPS which can confer glyphosate resistance. A DNA molecule encoding a mutant aroA gene can be obtained under ATCC accession number 39256, and the nucleotide sequence of the mutant gene is disclosed in U.S. Pat. No. 4,769,061 to Comai. A hygromycin B phosphotransferase gene from E. coli that confers resistance to glyphosate in tobacco callus and plants is described in Penaloza-Vazquez et al. (Plant Cell Reports, 14:482-487, 1995). European patent application No. 0 333 033 to Kumada et al., and U.S. Pat. No. ...

The bacterium Legionella pneumophila is the responsible agent for Legionnaires disease and has recently been shown to harbor a gene encoding a kinase that confers resistance to the aminoglycoside antibiotic spectinomycin (Suter, T. M., Viswanathan, V. K., and Cianciotto, N. P. (1997) Antimicrob. Agents Chemother. 41, 1385-1388). We report the overproduction, purification, and characterization of this spectinomycin kinase from an expressing system in Escherichia coli. The purified protein shows stringent substrate specificity for spectinomycin with Km = 21.5 microM and kcat = 24.2 s-1 and does not bind other aminoglycosides including kanamycin, amikacin, neomycin, butirosin, streptomycin, or apramycin. Purification of spectinomycin phosphate followed by characterization by mass spectrometry and 1H, 13C, and 31P NMR established the site of phosphorylation to be at the hydroxyl group at position 9. Thus this enzyme is designated APH(9)-Ia (where APH is aminoglycoside kinase). The enzyme was inactivated by

TY - JOUR. T1 - Substrate Recognition by a Colistin Resistance Enzyme from Moraxella catarrhalis. AU - Stogios, Peter J.. AU - Cox, Georgina. AU - Zubyk, Haley L.. AU - Evdokimova, Elena. AU - Wawrzak, Zdzislaw. AU - Wright, Gerard D.. AU - Savchenko, Alexei. N1 - Funding Information: We thank R. Di Leo for cloning. The structures presented were solved by the Center for Structural Genomics of Infectious Diseases (CSGID, http://csgid.org); this project has been funded in whole or in part with U.S. Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract Numbers HHSN272201200026C and HHSN272201700060C. This research was supported by the Ontario Research Fund Research Excellence (ORF-RE) Grant No. RE07-048 (to G. D. Wright and A. Savchenko). This research was also supported by a Canadian Institutes of Health Research grant (FRN-148463) and by a Canada Research Chair in Antibiotic Biochemistry ...

Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous genes polyadenylation sequence and provide transcript stability. However, the spectrum of mutations generated by these vectors appears largely restricted to the last intron of target loci due to nonsense-mediated mRNA decay (NMD) making them unsuitable for gene targeting applications. Here, we present novel poly A trap vectors that overcome the effect of NMD and also employ RNA instability sequences to improve splicing efficiency. The set of random insertions generated with these vectors show a significantly reduced insertional bias and the vectors can be targeted directly to a 5′ intron. We also show that this ...

Top 99% Antibacterial Pharmaceutical Raw Materials Neomycin sulfate CAS 1405-10-3 Quick Details for Neomycin Sulfate Neomycin sulfate Product Name: Neomycin sulfate Neomycin sulfate Synonyms: FRADIOMYCIN SULFATE;NEOMYCIN SULFATE;NEOMYCIN SULFATE,...

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The American Printing House for the Blind (APH) makes every attempt to ensure the accuracy and reliability of the data contained in the Freds Head articles; however, APH makes no warranty, guarantee, or promise, expressed or implied, concerning the content or accuracy of the information provided in Freds Head. APH does not endorse any technique, product, device, service, organization, or other information presented in Freds Head, other than products and services directly offered by APH. ...

In order to offer dental professionals high-level education during the pandwemic, CAPP will be hosting its two conferences online this year.

Kanamycin Sulfate question - posted in Microbiology: Hi, We have Kanamycin sulfate that we aliquoted into 100mg/mL in eppendorf tubes stored in the -20. When we are ready to make our LB+Kan, we thaw one. How long would you recommend leaving it out before having to discard it? Thanks, Adiel

This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found o

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Neo4j®, Neo Technology®, Cypher®, Neo4j® Bloom™ and Neo4j® Aura™ are registered trademarks of Neo4j, Inc. All other marks are owned by their respective companies ...